PLoS ONE最新文献

{"title":"Curcumin induces mitochondrial dysfunction-associated oxidative DNA damage in ovarian cancer cells.","authors":"Qi Bao, Zihan Wang, Tingting Yang, Xiao Su, Ying Chen, Lifen Liu, Qicheng Deng, Qingyang Liu, Changshun Shao, Weipei Zhu","doi":"10.1371/journal.pone.0319846","DOIUrl":"https://doi.org/10.1371/journal.pone.0319846","url":null,"abstract":"<p><p>Resistance to chemotherapeutic agents is a critical challenge for the clinical management of ovarian cancer. While curcumin has been reported to possess anti-cancer properties, how it exerts its anti-neoplastic effect on ovarian cancer cells remains to be explored. We here characterized the fate of human ovarian cancer cell lines HO8910 and OVCAR3 treated with curcumin. Cell proliferation, cell death, mitochondrial function, oxidative damage and tumor formation in nude mice were examined. Significant inhibition of proliferation and induction of apoptosis were observed in ovarian cells treated with curcumin. The cancer cells exhibit cell cycle arrest at G2/M phase, mitochondrial accumulation, mitochondrial oxidative stress and high level of DNA damage after curcumin treatment. This effect of curcumin is independent of the BRCA mutation status. Curcumin-induced proliferation inhibition and apoptosis were effectively attenuated by the application of antioxidant N-acetylcysteine (NAC), suggesting that curcumin exerts its anti-cancer effect by inflicting oxidative stress. Curcumin applied at 200 mg/kg intraperitoneal infusion daily also inhibited the growth, oxidative damage, and mitochondrial accumulation of tumor xenografts in vivo. Together, the results indicate that curcumin can exert its anti-tumor effect via inducing mitochondrial dysfunction-associated oxidative DNA damage and can be potentially used in combination with other DNA repair-interfering therapeutics, such as PARP inhibitor, in the treatment of ovarian cancer.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0319846"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weather extremes and their impact on crop transportation networks: Evidence from U.S. Midwestern elevators.","authors":"Theodoros Skevas, Wyatt Thompson, Benjamin Brown, Delmy Salin, Jesse Gastelle, Edgar Marcillo-Yepez","doi":"10.1371/journal.pone.0319815","DOIUrl":"https://doi.org/10.1371/journal.pone.0319815","url":null,"abstract":"<p><p>The grain price margins between buyers and sellers (i.e., basis spread) is influenced by the infrastructure used to transport crops from collection points to ports, which can be disrupted by weather extremes like floods and severe storms. Such disruptions are expected to become more frequent, potentially increasing food insecurity and impacting farm incomes. On average, the U.S. accounts for one-third of global corn and soybean production from 2012/13 to 2020/21, so the infrastructure to move crops from the main growing region to the nation's ports is critical to global crop and food markets. Despite the critical nature of these issues, there is limited research specifically examining the effects of weather extremes on the U.S. crop transportation network. This study investigates how weather extremes disrupt crop transportation networks, and, in turn, how those disruptions affect the basis spread of corn and soybeans. It uses basis spread data from nearly 5,000 U.S. midwestern corn and soybean elevators spanning from 2012 to 2020, along with natural disaster declarations to represent weather extremes affecting crop transportation. Using a three-step process, it calculates least cost transportation routes to a port, adjusts for weather disruptions, and integrates disaster, transportation cost, and control variables into a fixed effects, panel data model that explains variation in basis spread. Results show natural disasters, particularly flash floods and winter storms, negatively affect basis spread. The cost effects of natural disasters disrupting crop transportation routes further decrease basis spread. Strengthening crop transportation infrastructure to withstand flooding and winter storms could reduce disruptions in this network. These findings underscore the value of Federal and State policies that prioritize investments in resilient transportation infrastructure, particularly in regions prone to flash floods and winter storms. Strengthening this infrastructure could not only reduce the economic costs of weather disruptions but also affect farm income and food security.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0319815"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: An initiative to develop capability-adjusted life years in Sweden (CALY-SWE): Selecting capabilities with a Delphi panel and developing the questionnaire.","authors":"","doi":"10.1371/journal.pone.0321980","DOIUrl":"https://doi.org/10.1371/journal.pone.0321980","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0263231.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0321980"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a health index for stranded marine tetrapods.","authors":"André S Barreto, Karla R K Andrioli, Ana Paula F R L Bracarense, Marta J Cremer, Daphne W Goldberg, Guilherme Guerra-Neto","doi":"10.1371/journal.pone.0319250","DOIUrl":"https://doi.org/10.1371/journal.pone.0319250","url":null,"abstract":"<p><p>This study presents the development and validation of a Health Index (HI) to assess the overall health of stranded marine tetrapods (seabirds, sea turtles, and marine mammals). The HI is based on parameters collected during necropsies and histopathological analyses, including body condition, systemic conditions (macroscopic and by histopathology), cutaneous injuries, organized lesions, parasitosis, lymphoid depletion, and thyroid alterations. The HI was applied to data from 6,332 marine tetrapods stranded along the Brazilian coast. Results showed that the HI effectively differentiated between animals with good, fair, and poor health, as evaluated by independent veterinary assessments. Sea turtles exhibited the lowest average HI, suggesting poorer health conditions compared to seabirds and marine mammals. The HI has proven to be a valuable tool for monitoring the health of marine tetrapod populations. Using a quantitative measure of an animal's health enables the evaluation of spatial and temporal variations in health status and the identification of more subtle impacts on species or populations. Thus, the HI allows for an objective assessment and comparison of wildlife health, supporting conservation efforts and helping to identify potential threats.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0319250"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-induced cytotoxicity prediction in muscle cells, an application of the Cell Painting assay.","authors":"Roman Lambert, Eva Serrano Candelas, Pablo Aparicio, Aisling Murphy, Rafael Gozalbes, Howard Oliver Fearnhead","doi":"10.1371/journal.pone.0320040","DOIUrl":"https://doi.org/10.1371/journal.pone.0320040","url":null,"abstract":"<p><p>In silico toxicity prediction offers the chance of reducing or replacing most animal testing through the integration of large experimental assay datasets with the appropriate computational approaches. The use of Cell Painting to detect various phenotypic changes induced by chemicals is emerging as a powerful technique in toxicity prediction. However, most Cell Painting approaches use cancer cells that are less relevant for many toxicological endpoints, which may limit the usefulness of this data. In this study, a myoblast cell line is used to characterize cellular responses to a panel of 30 known myotoxicants. In place of traditional structural descriptors, here each perturbation is described by a fingerprint of calculated properties, deducted from the intensity, shape, or texture of individual cells. We show that these kinds of descriptors convey information to allow the prediction of the cellular viability and fate of cells in myoblasts and differentiated myotubes of the C2C12 cell line, and the clustering of drugs by their cytotoxicity responses.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320040"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Device-aided therapies (DATs) in Parkinson's disease (PD). The DATs-PD GETM Spanish Registry Protocol Study.","authors":"Diego Santos-García, Guillermo González-Ortega, Pilar Sánchez-Alonso, Anna Planas-Ballvé, Rocío García-Ramos, Iria Cabo, Marta Blázquez-Estrada, Álvaro Sánchez-Ferro","doi":"10.1371/journal.pone.0316052","DOIUrl":"https://doi.org/10.1371/journal.pone.0316052","url":null,"abstract":"<p><strong>Background and objective: </strong>Device-aided therapies (DATs) are treatments indicated for people with Parkinson´s disease (PwP) experiencing clinical fluctuations that remain suboptimal despite conventional medication. New DATs have recently emerged such as levodopa-entacapone-carbidopa intestinal gel infusion (LECIG) and subcutaneous infusion of foslevodopa/foscarbidopa (fLD/fCD). Understanding the differences between various DATs is essential.</p><p><strong>Patients and methods: </strong>We present here the protocol study of the DATs-PD GETM Spanish Registry. This is a descriptive, observational, prospective, multicenter, open study that is proposed as a clinical registry with progressive inclusion of PwP treated with a DAT in daily clinical practice conditions in more 40 centers from Spain for 10 years. The principal aim is to know the type of DAT that PwP in our country (Spain) receive. Specific objectives are to compare the clinical characteristics of the patients, the effectiveness, safety and tolerability, to identify predictors of a good response and to analyze the response by groups (gender, disease duration, phenotype, etc.). There is a baseline visit (V1; indication of the therapy), start visit (V2; initiation of the therapy) and follow-up visits at 6 months ±  3 months (V3_6M) and after this annually ±  3 months for 10 years (V3_12M, V3_24M, etc.).</p><p><strong>Results: </strong>The registry is on going. The first patient was included on April 10, 2024. Patient recruitment and follow-up will be conducted until 31/DEC/2033. It is estimated that the registry will include a minimum of 3,000 patients.</p><p><strong>Conclusion: </strong>The present study will help improve the care of PD patients treated with a DAT.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316052"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic factors for long-term mortality after surgery of left-sided infective endocarditis.","authors":"Se Ju Lee, Jung Ho Kim, Yongseop Lee, Sangmin Ahn, Jung Ah Lee, Jinnam Kim, Hyung Jung Oh, Jin Young Ahn, Su Jin Jeong, Jun Yong Choi, Joon-Sup Yeom, Nam Su Ku, Seung Hyun Lee","doi":"10.1371/journal.pone.0321068","DOIUrl":"https://doi.org/10.1371/journal.pone.0321068","url":null,"abstract":"<p><strong>Background: </strong>Infective endocarditis has low prevalence but a high mortality rate. Left-sided infective endocarditis (LSIE) has a higher mortality rate than right-sided infective endocarditis. Surgical treatment is occasionally considered for LSIE; however, few data are available on the long-term prognostic factors for LSIE after surgical treatment. This study investigated the risk factors for long-term mortality in LSIE patients who underwent surgical treatment.</p><p><strong>Methods: </strong>This retrospective study enrolled adult patients with LSIE who were admitted to Severance Hospital in South Korea and underwent surgical treatment from November 2005 to August 2017. The primary outcome was risk factors for overall all-cause mortality. Multivariable Cox regression analysis was performed to identify risk factors for long-term mortality of patients with LSIE who received surgical treatment.</p><p><strong>Results: </strong>This study enrolled 239 with LSIE who underwent surgery. The median follow-up period was 75.9 months, and there were 34 deaths (14.2%) during the study period. Multivariable Cox analysis showed that central nervous system complications (hazard ratio [HR]: 3.55, 95% confidence interval [CI]: 1.76-7.17, P <  0.001), chronic liver disease (CLD) (HR: 4.33, 95% CI: 1.57-11.91, P =  0.005), and age ≥  65 years (HR: 2.65, 95% CI: 1.28-5.51, P =  0.009) were risk factors for overall mortality. Kaplan-Meier analysis indicated a significant difference in survival between patients with and without CNS complications (P <  0.001, log-rank).</p><p><strong>Conclusion: </strong>Central nervous system complications, CLD, and older age were associated with long-term mortality in surgically treated patients with LSIE. Preventive strategies for CNS complications would improve the treatment of LSIE.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0321068"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis B vaccine coverage in health care students: a cross-sectional study in Vietnam.","authors":"Ly Trieu Vo, Dung Quoc Phan, Huy Gia Tran, Lam Thi Phuong Nguyen, Araba Gyan, Han Thi Ngoc Nguyen, Giao Huynh","doi":"10.1371/journal.pone.0320860","DOIUrl":"https://doi.org/10.1371/journal.pone.0320860","url":null,"abstract":"<p><p>Hepatitis B virus (HBV) remains a significant global health concern, with healthcare students being at elevated risk of infection during their internships in healthcare settings. This study aimed to determine the percentage of healthcare students vaccinated against hepatitis B and the factors associated with vaccination status. A cross-sectional study was conducted among healthcare students at the University of Medicine and Pharmacy at Ho Chi Minh City in Vietnam between February and May 2023 using a self-reported questionnaire. Participants were selected through stratified and random sampling. A multivariable analysis logistic regression was performed to determine the association between several factors with vaccination status. A total of 225 participants took part in the study. We found that 89.8% of the participants had received at least one dose of the HBV vaccine, while 63.3% had completed the entire vaccine schedule. Students with sufficient knowledge of HBV were 2.68 times more likely to be vaccinated (p <  0.05), while those practicing effective HBV prevention had 8.79 times higher vaccination rates (p <  0.001) compared to others. The rate of HBV vaccination among healthcare students remains suboptimal. Targeted health education that addresses knowledge gaps, enhances motivation, tackles vaccination barriers, and emphasizes preventive measures before internships could substantially improve vaccination coverage in this group.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320860"},"PeriodicalIF":2.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TsRNA-49-73-Glu-CTC: A promising serum biomarker in non-small cell lung cancer.","authors":"Chenyu Li, Shenjie Zhong, Juan Chen, Xiaofeng Mu","doi":"10.1371/journal.pone.0320187","DOIUrl":"10.1371/journal.pone.0320187","url":null,"abstract":"<p><strong>Objective: </strong>Lung cancer has the highest incidence and mortality rates globally, with the majority of cases classified as non-small cell lung cancer (NSCLC). Due to the absence of specific tumor biomarkers, most lung cancer cases are diagnosed at an advanced stage. Therefore, the identification of novel molecular biomarkers with high sensitivity and specificity for early diagnosis is deemed crucial for enhancing the treatment of NSCLC. Transfer RNA-derived small RNA (tsRNA) is closely associated with malignant tumors and holds promise as a potential biomarker for tumor diagnosis. This study aimed to investigate whether serum tsRNA could serve as a biomarker for NSCLC.</p><p><strong>Methods: </strong>Differentially expressed tsRNAs were identified through high-throughput sequencing of serum samples obtained from patients with NSCLC and healthy individuals. Additional serum samples were collected for validation using Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR). The diagnostic performance of these tsRNAs was assessed through Receiver Operating Characteristic (ROC) Curve Analysis. Furthermore, preliminary functional exploration was undertaken through cell experiments.</p><p><strong>Results: </strong>tsRNA-49-73-Glu-CTC is highly expressed in the serum of patients with NSCLC and demonstrates superior diagnostic value compared to commonly used tumor markers in clinical practice, such as Carcinoembryonic Antigen (CEA), Neuron-Specific Enolase (NSE), and Cytokeratin 19 Fragment (CYFRA). A combined diagnostic approach enhances the accuracy of NSCLC detection. Additionally, tsRNA-49-73-Glu-CTC is highly expressed in A549 cells, and transfection with a tsRNA-49-73-Glu-CTC inhibitor significantly reduces both proliferation and migration capabilities.</p><p><strong>Conclusions: </strong>tsRNA-49-73-Glu-CTC has the potential to serve as a novel molecular diagnostic biomarker for NSCLC and plays a significant role in the biological processes associated with NSCLC proliferation and migration.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320187"},"PeriodicalIF":2.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow-core PCF for terahertz sensing: A new approach for ethanol and benzene detection.","authors":"Md Golam Sadeque, Suchana Aktar Tithi, Md Safiul Islam, A H M Iftekharul Ferdous, Diponkar Kundu, Md Galib Hasan, Md Zakirul Islam Sarker","doi":"10.1371/journal.pone.0320805","DOIUrl":"10.1371/journal.pone.0320805","url":null,"abstract":"<p><p>Terahertz (THz) spectroscopy is becoming a powerful technique for non-destructive, label-free chemical sensing with applications ranging from medicinal research to security screening. Enhancing THz spectroscopy's sensitivity and selectivity is crucial to maximizing its potential. In this work, we offer a novel optical fiber design, square shape core PCF that is tailored to exploit improved optical features at exterior in the THz region. This analysis suggests that a square shape and three layers with square air apertures for the cladding and core would be ideal. The mathematical analysis is carried out at THz wave dissemination utilizing FEM and boundaries circumstance of the Perfectly Matched Layer. Using the simulation method, the constructed square PCF sensor achieves very high relative sensitivity (94.45%, 94.80%) at 2 THz for two compounds: ethanol (n = 1.354), and benzene (n = 1.36). On the other hand, the low confinement loss (CL) values for the same two compounds at 2 THz are 1.17 ×  10-05 dB/m, and 1.32 ×  1 0-05 dB/m, in that order. We also looked at the potential applications of this special fiber in a variety of fields, including environmental monitoring, chemical sensing, and biomedical diagnostics. The square PCF with square core has hitherto unexplored opportunities for the development of extremely selective and sensitive THz spectroscopic devices with important social consequences in domain of THz perception of chemicals.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320805"},"PeriodicalIF":2.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}