PLoS ONE最新文献

{"title":"A novel framework for increasing research transparency: Exploring the connection between diversity and innovation.","authors":"Timothy R Wojan, Dayton M Lambert","doi":"10.1371/journal.pone.0313826","DOIUrl":"10.1371/journal.pone.0313826","url":null,"abstract":"<p><p>A split sample/dual method research protocol is demonstrated to increase transparency while reducing the probability of false discovery. We apply the protocol to examine whether diversity in ownership teams increases or decreases the likelihood of a firm reporting a novel innovation using data from the 2018 United States Census Bureau's Annual Business Survey. Transparency is increased in three ways: 1) all specification testing and identifying potentially productive models is done in an exploratory subsample that 2) preserves the validity of hypothesis test statistics from de novo estimation in the holdout confirmatory sample with 3) all findings publicly documented in an earlier registered report and in this journal publication. Bayesian estimation procedures that leverage information from the exploratory stage included in the confirmatory stage estimation replace traditional frequentist null hypothesis significance testing. In addition to increasing statistical power by using information from the full sample, Bayesian methods directly estimate a probability distribution for the magnitude of an effect, allowing much richer inference. Estimated magnitudes of diversity along academic discipline, race, ethnicity, and foreign-born status dimensions are positively associated with innovation. A maximally diverse ownership team on these dimensions would be roughly six times more likely to report new-to-market innovation than a homophilic team.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0313826"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do you have enough space? Habitat selection of insectivorous cave-dwelling bats in fragmented landscapes of Eastern Amazon.","authors":"Valéria da C Tavares, Mariane S Ribeiro, Xavier Prous, Alice A Notini, Nathalia Y Kaku-Oliveira, Leandro M D Maciel, Sérgio Sales, Juliana M Longo, Flávia M Evangelista, Lucas Rabelo, Iuri V Brandi, Santelmo S Vasconcelos, Sonia S Talamoni, Guilherme Oliveira, Leonardo C Trevelin","doi":"10.1371/journal.pone.0296137","DOIUrl":"10.1371/journal.pone.0296137","url":null,"abstract":"<p><p>Individual movements of bats are triggered by their life requirements, limited by their recognition of the environment and risks of moving, and mediated by habitat selection. Mining adds fragmentation and heterogeneity to landscapes, with poorly understood consequences to the life activities of the bats. Cave dwelling bats spend most of their life cycles within caves, and as they constantly forage in external landscapes, their contribution in the input of organic matter to the caves is of paramount importance to the subterranean biodiversity. We investigated habitat selection by cave bats in a mosaic of Amazonian forests, iron-rich savannas and iron-caves, studying the movements of the aerial insect-catcher Furipterus horrens (Furipteridae) and of the foliage gleaning bat Lonchorhina aurita (Phyllostomidae), both cave-dependent in the area. We used radio telemetry to assess their use of habitats, under the prediction that these species prefer preserved habitats for their foraging bouts, avoiding human-modified habitats. We also anticipated larger areas and commuting distances for both species when foraging in disturbed landscapes. Thirty-one bats were monitored in conserved habitats, mining sites and pastures resulting in an average range of 415.1 ± 837.4 ha and average commuting distances of 2163 ± 2453 m (F. horrens) and 681.1 ± 1101 ha and 2781.5 ± 2333 m (L. aurita). Our results suggest that F. horrens are open space foragers, frequently recorded in cangas, and L. aurita are forest foragers that use all habitats proportionally to their availability in the landscape. We detected landscape-related effects mediated by seasonal variation in the maximum commuting distance performed by both species. These are the first radiotelemetry data available for F. horrens and L. aurita, delivering original baselines to understand their movement behaviors. This information sheds light into constraints related to the optimal and adjusted biological cycles of these two species and their range shifts under scenarios of disturbance and may subsidize programs for their conservation.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0296137"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adoption of AI writing tools among academic researchers: A Theory of Reasoned Action approach.","authors":"Mohammed A Al-Bukhrani, Yasser Mohammed Hamid Alrefaee, Mohammed Tawfik","doi":"10.1371/journal.pone.0313837","DOIUrl":"10.1371/journal.pone.0313837","url":null,"abstract":"<p><p>This research explores the determinants affecting academic researchers' acceptance of AI writing tools using the Theory of Reasoned Action (TRA). The impact of attitudes, subjective norms, and perceived barriers on researchers' intentions to adopt these technologies is examined through a cross-sectional survey of 150 researchers. Structural Equation Modeling (SEM) is employed to evaluate the measurement and structural models. Findings confirm the positive influence of favorable attitudes and subjective norms on intentions to use AI writing tools. Interestingly, perceived barriers did not significantly impact attitudes or intentions, suggesting that in the academic context, potential benefits may outweigh perceived obstacles to AI writing tool adoption. Contrarily, perceived barriers do not significantly affect attitudes and intentions directly. The TRA model demonstrates considerable explanatory and predictive capabilities, indicating its effectiveness in understanding AI writing tool adoption among researchers. The study's diverse sample across various disciplines and career stages provides insights that may be generalizable to similar academic contexts, though further research with larger samples is needed to confirm broader applicability. Results offer practical guidance for tool developers, academic institutions, and publishers aiming to foster responsible and efficient AI writing tool use in academia. Findings suggest strategies such as demonstrating clear productivity gains, establishing AI Writing Tool programs, and developing comprehensive training initiatives could promote responsible adoption. Strategies focusing on cultivating positive attitudes, leveraging social influence, and addressing perceived barriers could be particularly effective in promoting adoption. This pioneering study investigates researchers' acceptance of AI writing tools using a technology acceptance model, contributing to the understanding of technology adoption in professional contexts and highlighting the importance of field-specific factors in examining adoption intentions and behaviors.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0313837"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the structure and robustness of the global semiconductor trade network.","authors":"Long Li, Hua Wang, Zhiyi Li, Shaodong Hu","doi":"10.1371/journal.pone.0313162","DOIUrl":"10.1371/journal.pone.0313162","url":null,"abstract":"<p><p>Amidst the global restructuring of the semiconductor supply chain, this paper constructs a global semiconductor trade network (2007, 2012, 2017, 2021) encompassing three segments (raw materials, equipment, and finished components), based on the CEPII database. After initially exploring trade flows among different regions, the paper conducts an in-depth analysis of the network's overall structure and the significance of its nodes. Furthermore, the evolution of the trade network's community structure is discussed and its robustness and dynamics over recent years are assessed through computer program simulation. The findings are as follows: First, semiconductor trade flows are concentrated primarily among a few regions in Asia, US, and EU. Second, the network has grown in size and exhibits significant \"small-world\" characteristics in all segments, deviating from the typical \"sparsity\" seen in large-scale networks. Third, Japan, the US, and a few European regions wield significant influence in semiconductor materials and equipment trade, while Asian economies such as Chinese mainland, Chinese Taiwan, and Korea dominate semiconductor components trade. Fourth, the raw materials trade network has diversified in recent years, while the trade networks for equipment and finished components remain in a state of continuous \"polarization.\" Fifth, the semiconductor trade network demonstrates robustness against random attacks but collapses quickly under targeted attacks. Among the three segments, the trade network of finished components, being larger in scale, exhibits greater resilience against both random and targeted attacks. This paper not only enhances the construction of the global semiconductor trade network but also introduces a dynamic perspective, offering deeper insights into its structure and robustness. The insights gained from this analysis provide valuable guidance for policymakers and companies, especially amidst rapid technological change and geopolitical tensions.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0313162"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic insights into the risk of hip osteoarthritis on stroke: A single-variable and multivariable Mendelian randomization.","authors":"Zhengze Zhang, Yanan Lian, Yuewen He, Hao Liu, Kai Meng, Yong Wang, Wuhua Ma","doi":"10.1371/journal.pone.0313032","DOIUrl":"10.1371/journal.pone.0313032","url":null,"abstract":"<p><strong>Background: </strong>Hip osteoarthritis has been identified as a potential risk factor for stroke, with previous studies have demonstrated an association between hip osteoarthritis and stroke. This study aims to further elucidate the causal relationship between the two, employing Two-Sample and Multivariable Mendelian randomization methods.</p><p><strong>Methods: </strong>SNPs, derived from two extensive GWAS, served as instruments in exploring the association between genetically predicted hip osteoarthritis and stroke risk, utilizing two-sample Mendelian randomization. In Multivariable Mendelian randomization, factors such as cigarettes per day, alcoholic drinks per week, hypertension, body mass index, type 2 diabetes, C-reactive protein, rheumatoid arthritis were incorporated to further account for the independent causal effects of multiple correlated exposures.</p><p><strong>Results: </strong>Two-sample Mendelian randomization analysis revealed that hip osteoarthritis exerts a potential causal effect on any stroke, any ischemic stroke, and cardioembolic stroke, while it did not influence large artery stroke and small vessel stroke. Multivariable MR analysis indicated that the causal effect of hip osteoarthritis on any ischemic stroke and cardioembolic stroke was no longer evident after adjusting for C-reactive protein, and similarly, the effect on any ischemic stroke was not observed after adjusting for type 2 diabetes. However, the effects on any stroke, any ischemic stroke, and cardioembolic stroke remained significant after adjustments for hypertension, alcoholic drinks per week, cigarettes per day, body mass index, and rheumatoid arthritis.</p><p><strong>Conclusion: </strong>The study demonstrated that elevated hip osteoarthritis, as predicted by genetic factors, was potential associated with an increased risk of any stroke, any ischemic stroke, and cardioembolic stroke, but showed no correlation with hypertension, alcoholic drinks per week, cigarettes per day, type 2 diabetes, C-reactive protein, body mass index levels, and rheumatoid arthritis.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0313032"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of pulmonary surfactant with budesonide in premature infants: A systematic review and meta-analysis.","authors":"Nanthida Phattraprayoon, Bing Tan, Mingkwan Na Takuathung","doi":"10.1371/journal.pone.0312561","DOIUrl":"10.1371/journal.pone.0312561","url":null,"abstract":"<p><p>Pulmonary surfactant (PS) is one of the main treatment for neonates with respiratory distress syndrome (RDS). Budesonide has recently been studied as an additional treatment in such cases, but there is limited evidence supporting this. This study was implemented to determine the efficacy of PS combined with budesonide in premature infants. To achieve this, we conducted a systematic review and meta-analysis of randomized controlled trials by searching PubMed, Scopus, Embase, and the Cochrane Library from inception until July 12, 2024. We utilized a random-effects model to calculate the risk ratio and mean differences (MDs) with 95% confidence intervals (CIs) for the clinical outcomes of PS with budesonide versus PS alone. We used the GRADE approach to assess the quality of the evidence. We included 26 randomized controlled trials with a total of 2701 patients in the analysis. Treatments of PS with budesonide and PS alone were compared in all trials. PS with budesonide reduced bronchopulmonary dysplasia (BPD) incidence (risk ratio, 0.61; 95% CI, 0.51, 0.73), duration of mechanical or invasive mechanical ventilation (MD, -2.21 days; 95% CI, -2.72, -1.71), duration requiring oxygen (MD, -5.86 days; 95% CI, -8.44, -3.29), and hospitalization time (MD, -5.61 days; 95% CI, -8.65, -2.56). These results were based on low to very low evidence certainty. Only moderate-to-severe BPD or severe BPD showed a significant reduction when PS was used in conjunction with budesonide, a finding supported by moderate evidence certainty. Our study showed that the administration of PS with budesonide significantly improved respiratory outcomes, including the incidence of BPD, duration of mechanical or invasive mechanical ventilation, duration requiring oxygen, and hospitalization time in preterm infants, without short-term adverse drug events. However, the evidence certainty was mostly low to very low.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0312561"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing stroke disease classification through machine learning models via a novel voting system by feature selection techniques.","authors":"Mahade Hasan, Farhana Yasmin, Md Mehedi Hassan, Xue Yu, Soniya Yeasmin, Herat Joshi, Sheikh Mohammed Shariful Islam","doi":"10.1371/journal.pone.0312914","DOIUrl":"10.1371/journal.pone.0312914","url":null,"abstract":"<p><p>Heart disease remains a leading cause of mortality and morbidity worldwide, necessitating the development of accurate and reliable predictive models to facilitate early detection and intervention. While state of the art work has focused on various machine learning approaches for predicting heart disease, but they could not able to achieve remarkable accuracy. In response to this need, we applied nine machine learning algorithms XGBoost, logistic regression, decision tree, random forest, k-nearest neighbors (KNN), support vector machine (SVM), gaussian naïve bayes (NB gaussian), adaptive boosting, and linear regression to predict heart disease based on a range of physiological indicators. Our approach involved feature selection techniques to identify the most relevant predictors, aimed at refining the models to enhance both performance and interpretability. The models were trained, incorporating processes such as grid search hyperparameter tuning, and cross-validation to minimize overfitting. Additionally, we have developed a novel voting system with feature selection techniques to advance heart disease classification. Furthermore, we have evaluated the models using key performance metrics including accuracy, precision, recall, F1-score, and the area under the receiver operating characteristic curve (ROC AUC). Among the models, XGBoost demonstrated exceptional performance, achieving 99% accuracy, precision, F1-Score, 98% recall, and 100% ROC AUC. This study offers a promising approach to early heart disease diagnosis and preventive healthcare.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0312914"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of agricultural employment during the COVID-19 pandemic: A spatial analysis of Brazilian municipalities.","authors":"Patrícia Batistella, Luan Marca, Fernanda Castilhos França de Vasconcellos, Eduardo Rodrigues Sanguinet, Augusto Mussi Alvim, Adelar Fochezatto","doi":"10.1371/journal.pone.0316489","DOIUrl":"10.1371/journal.pone.0316489","url":null,"abstract":"<p><p>The impact of COVID-19 has extended beyond the health toll it has taken on populations. The global economy has experienced significant downturns, with unemployment rates reaching unprecedented highs for this century. Nonetheless, the agricultural sector has been uniquely affected by the pandemic, particularly given its crucial role in food supply. This study sought to examine the effects of the COVID-19 pandemic on agricultural employment in Brazilian municipalities. To achieve this, we employed a dataset spanning the period before and during the pandemic (2018-2021). Spatial econometrics was utilized to identify potential spatial factors linked to this occurrence. Notably, the findings reveal that the transfer policies implemented by the Brazilian Federal Government have had a positive impact on agricultural employment in Brazilian municipalities, challenging the common assumption that such measures might discourage job-seeking efforts. Moreover, the results suggest that the agricultural sector has managed to absorb individuals displaced from other sectors of the economy, demonstrating remarkable resilience in the face of economic challenges.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0316489"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azithromycin removal from water via adsorption on drinking water sludge-derived materials: Kinetics and isotherms studies.","authors":"Camilo C Castro-Jiménez, Julio C Saldarriaga-Molina, Edwin F García, Ricardo A Torres-Palma, Nancy Acelas","doi":"10.1371/journal.pone.0316487","DOIUrl":"10.1371/journal.pone.0316487","url":null,"abstract":"<p><p>In this study, we utilized drinking water treatment sludge (WTS) to produce adsorbents through the drying and calcination process. These adsorbents were then evaluated for their ability to remove azithromycin (AZT) from aqueous solutions. The L-500 adsorbent, derived from the calcination (at 500°C) of WTS generated under conditions of low turbidity in the drinking water treatment plant, presented an increase in the specific surface area from 70.745 to 95.471 m2 g-1 and in the total pore volume from 0.154 to 0.211 cm3 g-1, which resulted in a significant AZT removal efficiency of 65% in distilled water after 60 min of treatment. In synthetic wastewater, the rate of AZT removal increased to 80%, in comparison, in a real effluent of a municipal wastewater treatment plant, an AZT removal of 56% was obtained. Kinetic studies revealed that the experimental data followed the pseudo-second-order model (R2: 0.993-0.999, APE: 0.07-1.30%, and Δq: 0.10-2.14%) suggesting that chemisorption is the limiting step in the adsorption using L-500. This finding aligns with FTIR analysis, which indicates that adsorption mechanisms involve π-π stacking, hydrogen bonding, and electrostatic interactions. The equilibrium data were analyzed using the nonlinear Langmuir, Freundlich, and Langmuir-Freundlich isotherms. The Langmuir-Freundlich model presented the best fitting (R2: 0.93, APE: 2.22%, and Δq: 0.06%) revealing numerous interactions and adsorption energies between AZT and L-500. This adsorbent showed a reduction of 19% in its AZT removal after four consecutive reuse cycles. In line with the circular economy principles, our study presents an interesting prospect for the reuse and valorization of WTS. This approach not only offers an effective adsorbent for AZT removal from water but also represents a significant step forward in advancing sustainable water treatment solutions within the framework of the circular economy.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0316487"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical and biochemical evidence for the role of acetate kinases (AckAs) in an acetogenic pathway in pathogenic spirochetes.","authors":"Ranjit K Deka, Shih-Chia Tso, Wei Z Liu, Chad A Brautigam","doi":"10.1371/journal.pone.0312642","DOIUrl":"10.1371/journal.pone.0312642","url":null,"abstract":"<p><p>Unraveling the metabolism of Treponema pallidum is a key component to understanding the pathogenesis of the human disease that it causes, syphilis. For decades, it was assumed that glucose was the sole carbon/energy source for this parasitic spirochete. But the lack of citric-acid-cycle enzymes suggested that alternative sources could be utilized, especially in microaerophilic host environments where glycolysis should not be robust. Recent bioinformatic, biophysical, and biochemical evidence supports the existence of an acetogenic energy-conservation pathway in T. pallidum and related treponemal species. In this hypothetical pathway, exogenous D-lactate can be utilized by the bacterium as an alternative energy source. Herein, we examined the final enzyme in this pathway, acetate kinase (named TP0476), which ostensibly catalyzes the generation of ATP from ADP and acetyl-phosphate. We found that TP0476 was able to carry out this reaction, but the protein was not suitable for biophysical and structural characterization. We thus performed additional studies on the homologous enzyme (75% amino-acid sequence identity) from the oral pathogen Treponema vincentii, TV0924. This protein also exhibited acetate kinase activity, and it was amenable to structural and biophysical studies. We established that the enzyme exists as a dimer in solution, and then determined its crystal structure at a resolution of 1.36 Å, showing that the protein has a similar fold to other known acetate kinases. Mutation of residues in the putative active site drastically altered its enzymatic activity. A second crystal structure of TV0924 in the presence of AMP (at 1.3 Å resolution) provided insight into the binding of one of the enzyme's substrates. On balance, this evidence strongly supported the roles of TP0476 and TV0924 as acetate kinases, reinforcing the hypothesis of an acetogenic pathway in pathogenic treponemes.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 1","pages":"e0312642"},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}