Physiological Reports最新文献_第5页

Induction of cardiac alternans in human iPS-derived cardiomyocytes through β-adrenergic receptor stimulation. 通过β-肾上腺素能受体刺激诱导人ips源性心肌细胞的心脏交替。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70152

Yuto Hinata, Daisuke Sasaki, Katsuhisa Matsuura, Tatsuya Shimizu

{"title":"Induction of cardiac alternans in human iPS-derived cardiomyocytes through β-adrenergic receptor stimulation.","authors":"Yuto Hinata, Daisuke Sasaki, Katsuhisa Matsuura, Tatsuya Shimizu","doi":"10.14814/phy2.70152","DOIUrl":"10.14814/phy2.70152","url":null,"abstract":"Cardiac alternans (C-ALT) is a phenomenon of alternating strong and weak contractions in the heart and is considered a risk factor for the development of heart failure and arrhythmias. However, no model has been reported that can induce C-ALT in vitro using human cells, and the developmental mechanism of C-ALT has not been studied using human cells. In this study, we successfully induced C-ALT in vitro using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). By stimulating β-adrenergic receptor with isoproterenol on hiPSC-CMs cultured in atmospheric condition (with ~0.04% CO2), contractility and calcium transient were observed to alternately increase and decrease with each beat. In contrast, C-ALT was not induced in hiPSC-CMs cultured at 5% CO2 concentration. Since previous studies have linked C-ALT to problems with calcium regulation in the sarcoplasmic reticulum (SR), we exposed hiPSC-CMs to compounds that alter SR Ca2+ loading and analyzed their contractile responses. The results showed that exposure to verapamil, thapsigargin, and ryanodine either suppressed or eliminated C-ALT. In contrast, omecamtiv mecarbil and blebbistatin, which alter contractility without SR Ca2+ loading, did not induce or suppress C-ALT. These results suggest that C-ALT in hiPSC-CMs induced by isoproterenol may be due to abnormal regulation of the ryanodine receptor's opening and closing caused by excessive Ca2+ load in the SR from β-adrenergic receptor stimulation.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 24","pages":"e70152"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal changes in thiol-oxidized plasma albumin are associated with recovery from exercise-induced muscle damage after a marathon. 巯基氧化血浆白蛋白的时间变化与马拉松后运动引起的肌肉损伤的恢复有关。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70155

Christopher James, Erin M Lloyd, Peter G Arthur

{"title":"Temporal changes in thiol-oxidized plasma albumin are associated with recovery from exercise-induced muscle damage after a marathon.","authors":"Christopher James, Erin M Lloyd, Peter G Arthur","doi":"10.14814/phy2.70155","DOIUrl":"10.14814/phy2.70155","url":null,"abstract":"Exercise-induced muscle damage (EIMD) can affect athlete performance and is a risk factor for major muscle injury. The temporal profile of thiol-oxidized albumin, a marker of oxidative stress, has shown potential in assessing recovery from EIMD in non-athletically trained participants but not yet in trained participants. Our primary aim was to assess whether there are changes in the level of thiol-oxidized albumin after a marathon in athletically trained participants. Twenty participants completed a marathon and collected daily dried blood spots from 3 days prior to and 7 days after the marathon to measure thiol-oxidized albumin using a novel methodology (OxiDx). Participants were also assessed for indirect markers of EIMD prior to and on days 2 and 5 post-marathon. The level of thiol-oxidized albumin peaked at 2 days and remained elevated until 5 days after the marathon and correlated with indirect measures of EIMD. Furthermore, time of recovery for thiol-oxidized albumin varied between participants, some recovered at 3 days post-marathon whereas others extended beyond 7 days post marathon. Tracking temporal changes in the level of thiol-oxidized albumin has potential to be useful in managing recovery from EIMD in athletes, particularly considering the ease of the OxiDx methodology.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 24","pages":"e70155"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11679699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The epigenetic mechanisms of adaption to the hot and humid climate in Hu sheep (Ovis aries). 湖羊适应湿热气候的表观遗传机制。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.16164

Hangxing Ren, Jing Jiang, Jie Li, Xiaoyan Sun, Cancan Chen, Liangjia Liu, Shipeng Lv, Minghao Qu, Yuxue Fan, Peng Zhou, Gaofu Wang

{"title":"The epigenetic mechanisms of adaption to the hot and humid climate in Hu sheep (Ovis aries).","authors":"Hangxing Ren, Jing Jiang, Jie Li, Xiaoyan Sun, Cancan Chen, Liangjia Liu, Shipeng Lv, Minghao Qu, Yuxue Fan, Peng Zhou, Gaofu Wang","doi":"10.14814/phy2.16164","DOIUrl":"10.14814/phy2.16164","url":null,"abstract":"Hu sheep is characterized by its excellent fecundity and high adaptability to various ecological environments. To reveal the molecular basis involved in Hu sheep, we first examined the 10 index of neuroendocrine and metabolism in blood in Hu sheep during non-stress period (April-May) and stress period (July-August) using ELISA, including CRH, adrenocorticotropic hormone (ACTH), cortisol, aldosterone, adrenaline, T3,T4, SOD, GSH-PX, and T-AOC. Then we conducted the Whole genome DNA methylation sequencing in blood and performed the comparative analysis of global DNA methylation between the non-stress period and the stress period. Our results demonstrated that among the 10 index tested in blood, only ACTH, T3, and T-AOC were significantly changed (p < 0.01) in Hu sheep between two periods. This indicates Hu sheep's special adaptability to the high hygrothermal environment takes the decrease of metabolic level and total antioxidant capacity as compensation, which differ obviously from other intolerant hygrothermal animals. At the epigenetic level, differential methylation of TPO, ADCY9, PRKACB, and CREB5 play important roles in excellent resistance to hygrothermel environment in Hu sheep by modulation of the secretion of in neuroendocrine hormones (T3, ACTH) and thermogenesis. These findings are valuable for breeding the novel animal breeds resistant to climate stress.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 24","pages":"e16164"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of physical exercise intensity in motor-cognitive training of adults using the SKILLCOURT technology. 使用SKILLCOURT技术调节成人运动认知训练中的体育锻炼强度。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70136

Gülsa Erdogan, Bettina Karsten, Lutz Vogt, Andreas Mierau, Thorben Hülsdünker

{"title":"Modulation of physical exercise intensity in motor-cognitive training of adults using the SKILLCOURT technology.","authors":"Gülsa Erdogan, Bettina Karsten, Lutz Vogt, Andreas Mierau, Thorben Hülsdünker","doi":"10.14814/phy2.70136","DOIUrl":"10.14814/phy2.70136","url":null,"abstract":"Motor-cognitive training and exergaming often only reach low-to-medium intensities that limits their training efficiency. This study evaluated the physiological profile of different exercises on a novel motor-cognitive training technology designed to cover a broad range of exercise intensities. Twenty-six healthy trained adults (17 males, 23.7 ± 3.8 years) performed five motor-cognitive training tasks on the SKILLCOURT technology. Oxygen consumption (VO2), heart rate (HR), blood [lactate], perceived physical exertion (RPE) responses, and metabolic equivalent (MET) were assessed and compared to an incremental treadmill ramp test determining the maximal oxygen consumption (VO2max) and maximal heart rate (HRmax). Computer-based cognitive training served as control condition. Motor-cognitive exercises reached a higher %VO2max and %HRmax levels when compared to computer-based training (p < 0.001). Average intensity varied significantly between motor-cognitive tasks, with %VO2max ranging from 22% to 81% (p < 0.001), %HRmax from 49% to 89% (p < 0.001), METs from 3.57 to 13.37 (p < 0.001), blood [lactate] from 0.93 to 7.81 mmol·L-1 (p < 0.001), and RPE from 8.5 to 16.4 (p < 0.001). Motor-cognitive training covers a wide range of exercise intensities. This supports individual training subscription and allows high-intensity training to facilitate cardio-vascular adaptations and neural plasticity.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70136"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11620832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum proteome profiling of plateau acclimatization in men using Olink proteomics approach. 使用Olink蛋白质组学方法分析男性高原适应的血清蛋白质组学。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70091

Jingyu Pan, Yue Dong, Zhihao Zou, Tianyan Gu, Ling Chen, Kai Li, Li Wang, Qinghai Shi

{"title":"Serum proteome profiling of plateau acclimatization in men using Olink proteomics approach.","authors":"Jingyu Pan, Yue Dong, Zhihao Zou, Tianyan Gu, Ling Chen, Kai Li, Li Wang, Qinghai Shi","doi":"10.14814/phy2.70091","DOIUrl":"10.14814/phy2.70091","url":null,"abstract":"Plateau acclimatization involves adaptive changes in the body's neurohumoral regulation and metabolic processes due to hypoxic conditions at high altitudes. This study utilizes Olink targeted proteomics to analyze serum protein expression differences in Han Chinese individuals acclimatized for 6 months-1 year at 4500 and 5300 m altitudes, compared to those residing at sea level. The objective is to elucidate the proteins' roles in tissue and cellular adaptation to hypoxia. We identified 54 metabolism-related differentially expressed proteins (DEPs) in the serum of the high-altitude group versus the sea-level group, comprising 20 significantly upregulated and 34 downregulated proteins. Notably, 2 proteins were upregulated and 11 downregulated at both 4500 and 5300 m altitudes. The top three protein correlations among DEPs included CRKL with CA13, RNASE3 with NADK, and NADK with APEX1, alongside APLP1 with CTSH, CTSH with SOST, and CTSH with NT-proBNP in inverse correlations. KEGG enrichment analysis indicated significant DEP involvement in various metabolic pathways, particularly those associated with hypoxic cellular metabolism like glycolysis/gluconeogenesis and the HIF-1 signaling pathway. Correlation with clinical phenotypes showed positive associations of SOST, RNASE3, CA13, NADK, and CRKL with SaO2 and negative correlations with Hemoglobin and Hematocrit; ALDH1A1 positively correlated with Triglyceride; and SDC4 inversely correlated with Uric acid levels. This study provides insights into specific DEPs linked to metabolic adaptations in high-altitude acclimatized individuals, offering a foundation for understanding acclimatization mechanisms and potential therapeutic targets.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 24","pages":"e70091"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between muscle sympathetic nerve activity and serum fatty acid binding protein 4 at rest and during isometric handgrip exercise. 静息和等长握力运动时肌肉交感神经活动与血清脂肪酸结合蛋白4的关系。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70122

Tadayuki Hirai, Takuto Hamaoka, Hisayoshi Murai, Hiroyuki Sugimoto, Yusuke Mukai, Ayano Nomura, Takashi Kusayama, Tatsunori Ikeda, Shinichiro Takashima, Takeshi Kato, Kenji Sakata, Soichiro Usui, Shigeo Takata, Masayuki Takamura

{"title":"The relationship between muscle sympathetic nerve activity and serum fatty acid binding protein 4 at rest and during isometric handgrip exercise.","authors":"Tadayuki Hirai, Takuto Hamaoka, Hisayoshi Murai, Hiroyuki Sugimoto, Yusuke Mukai, Ayano Nomura, Takashi Kusayama, Tatsunori Ikeda, Shinichiro Takashima, Takeshi Kato, Kenji Sakata, Soichiro Usui, Shigeo Takata, Masayuki Takamura","doi":"10.14814/phy2.70122","DOIUrl":"10.14814/phy2.70122","url":null,"abstract":"Fatty acid binding protein 4 (FABP4) is highly expressed in adipocytes. Lipolysis, caused by an elevated adrenergic input, has been suggested to contribute to elevated serum FABP4 levels in patients with cardiovascular diseases. However, the relationship between the serum FABP4 and efferent sympathetic nerve activity remains poorly understood. Twenty-one healthy subjects (average age, 29.1 years; 15 men) performed an isometric handgrip (HG) exercise at 30% of the maximal voluntary contraction until they were fatigued. The beat-by-beat heart rate (HR), blood pressure (BP), and muscle sympathetic nerve activity (MSNA) were recorded. Blood samples were collected at rest and at the time of peak fatigue. The MSNA, HR, and systolic BP were significantly increased by the HG exercise (all, p < 0.05). MSNA was obtained from 14 patients. The change in the FABP4 on HG exercise was significantly correlated with the change in MSNA (bursts/100 heartbeats) (R = 0.808, p < 0.001) but not with changes in other parameters, which might, in part, reflect an association of efferent sympathetic drive with FABP4. Meanwhile, resting FABP4 levels were not associated with any parameters including MSNA, in healthy individuals. Future studies on patients with elevated sympathetic activity are warranted to examine the relationship further.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 24","pages":"e70122"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diastolic dysfunction evaluation by cardiovascular magnetic resonance derived E, a, e': Comparison to echocardiography. 通过心血管磁共振得出的 E、a、e'评估舒张功能障碍：与超声心动图的比较。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70078

Jérôme Lamy, Jie Xiang, Nimish Shah, Jennifer M Kwan, Yekaterina Kim, Krishna Upadhyaya, Samuel W Reinhardt, Judith Meadows, Robert L McNamara, Lauren A Baldassarre, Dana C Peters

{"title":"Diastolic dysfunction evaluation by cardiovascular magnetic resonance derived E, a, e': Comparison to echocardiography.","authors":"Jérôme Lamy, Jie Xiang, Nimish Shah, Jennifer M Kwan, Yekaterina Kim, Krishna Upadhyaya, Samuel W Reinhardt, Judith Meadows, Robert L McNamara, Lauren A Baldassarre, Dana C Peters","doi":"10.14814/phy2.70078","DOIUrl":"10.14814/phy2.70078","url":null,"abstract":"Transthoracic echocardiography (TTE) is the first-line and most useful imaging modality for evaluating diastolic dysfunction (DD). Cardiovascular magnetic resonance (CMR) has not been fully evaluated for this task. We investigated the utility of CMR for DD evaluation.Thirty-one patients with a recent TTE (within 4 months) were prospectively enrolled, along with 12 healthy age-matched subjects. CMR imaging was performed at 1.5 T to assess diastolic function by quantifying mitral inflow velocities (E and A), mitral annular velocities (e'), and left atrial volume (LAVi). Measurements by TTE and CMR were compared using regression. The diagnostic accuracy of CMR for DD was determined.CMR derived E, A, E/A, e' and E/e' all correlated moderately to strongly with TTE, and more strongly when comparing studies performed closer in time (E: r = 0.68, E deceleration time: r = 0.82, A: r = 0.78, e' r = 0.75, E/e': r = 0.80, p = 0.001; LAVi: r = 0.79, p < 0.001; E/A: r = 0.82, p < 0.001, n = 14 within 45 days). Using CMR criteria analogous to TTE, there was 82% (23/28) agreement regarding the presence of DD (95% CI [63 to 93%]), with 100% sensitivity and 75% specificity, and 71% (20/28) agreement in the absolute DD grade.CMR can evaluate diastolic function, with overall strong agreement to TTE.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70078"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise stress echocardiography shows impaired left ventricular function after hospitalization with COVID-19 without overt myocarditis: A pilot study. 运动负荷超声心动图显示 COVID-19 住院后左心室功能受损，但无明显心肌炎：一项试验性研究。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70138

Robert E Goldstein, Edward A Hulten, Thomas B Arnold, Victoria M Thomas, Andrew Heroy, Erika N Walker, Keiko Fox, Hyun Lee, Joya Libbus, Bethelhem Markos, Maureen N Hood, Travis E Harrell, Mark C Haigney

{"title":"Exercise stress echocardiography shows impaired left ventricular function after hospitalization with COVID-19 without overt myocarditis: A pilot study.","authors":"Robert E Goldstein, Edward A Hulten, Thomas B Arnold, Victoria M Thomas, Andrew Heroy, Erika N Walker, Keiko Fox, Hyun Lee, Joya Libbus, Bethelhem Markos, Maureen N Hood, Travis E Harrell, Mark C Haigney","doi":"10.14814/phy2.70138","DOIUrl":"10.14814/phy2.70138","url":null,"abstract":"Usual clinical testing rarely reveals cardiac abnormalities persisting after hospitalization for COVID. Such testing may overlook residual changes causing increased adverse cardiac events post-discharge. To clarify status post-hospitalization, we related exercise stress echocardiography (ESE) in 15 recovering patients (RP) age 30-63 without myocarditis to matching published data from healthy subjects (HS). RP exercise, average duration 8.2 ± 2.2 SD, was halted by dyspnea or fatigue. RP baselines matched HS except for higher heart rate. At peak stress, RP had significantly lower mean left ventricular (LV) ejection fraction (67% ± 7 vs. 73% ± 5, p < 0.0017) and higher peak early mitral inflow velocity/early mitral annular velocity (E/e', 9.1 ± 2.5 vs. 6.6 ± 2.5, p < 0.006) compared with HS performing equal exercise (8.5 ± 2.6 min). Thus, when stressed, patients without known cardiac impairment showed diminished systolic contractile function and diastolic LV compliance vs. HS. RP peak heart rate was significantly higher (172 ± 18 vs. 153 ± 20); peak systolic blood pressure trended higher (192 ± 31 vs. 178 ± 19). Pulmonary artery systolic pressures among RP remained normal. ESE uniquely identified residual abnormality in cardiac contractile function not evident unstressed, exposing previously unrecognized residual influence of COVID-19. This may reflect autonomic dysfunction, microvascular disease, or diffuse interstitial changes; these results may have implications for clinical management and later prognosis.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70138"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal pressure-flow relationship and renin activation in a porcine model comparing unilateral and bilateral renal artery stenosis. 比较单侧和双侧肾动脉狭窄的猪肾压力-血流关系和肾素激活。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70082

Benny Drieghe, Gunther van Loon, Sabrina Stuyvaert, Marc L De Buyzere, Thierry Bové, Tine De Backer

{"title":"Renal pressure-flow relationship and renin activation in a porcine model comparing unilateral and bilateral renal artery stenosis.","authors":"Benny Drieghe, Gunther van Loon, Sabrina Stuyvaert, Marc L De Buyzere, Thierry Bové, Tine De Backer","doi":"10.14814/phy2.70082","DOIUrl":"10.14814/phy2.70082","url":null,"abstract":"Because renal artery stenosis (RAS) often presents bilateral, we sought to investigate the renal pressure-flow relationship and its relation to renin release, in the presence of a contralateral significant stenosis. A porcine model of graded unilateral RAS in the presence of a significant contralateral stenosis was created. The severity of the stenosis was expressed as the ratio between distal renal pressure (Pd) and aortic pressure (Pa). Pd and renal flow velocity were continuously measured using a combined pressure-flow wire (Combowire®). Hemodynamic measurements and blood sampling for renin, angiotensin, and aldosterone were performed in baseline conditions and during progressive balloon inflation in the renal artery leading to stepwise 5% Pd decrements. Resistive index (RI) was computed as (1-(End Diastolic V/Maximum Peak Systolic V))*100. A decrease of average peak flow velocity (APV) was observed when distal renal perfusion pressure decreased by 25% and was associated with activation of renin secretion. The RI decreased already for minimal changes in Pd/Pa ratio. In an animal model of unilateral graded RAS in the presence of a significant contralateral stenosis, a 25% decrease in perfusion pressure results in a significant decrease in distal renal flow, causing a more pronounced upregulation of renin secretion when compared to a model of graded unilateral RAS without contralateral significant RAS.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70082"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Health implications of racial differences in serum growth differentiation factor levels among men with obesity. 肥胖男性血清生长分化因子水平的种族差异对健康的影响。

IF 2.2

Physiological Reports Pub Date : 2024-12-01 DOI: 10.14814/phy2.70124

Siresha Bathina, Virginia Fuenmayor Lopez, Mia Prado, Elliot Ballato, Georgia Colleluori, Maryam Tetlay, Dennis Tan Villareal, Sanjay Mediwala, Rui Chen, Clifford Qualls, Reina Armamento-Villareal

{"title":"Health implications of racial differences in serum growth differentiation factor levels among men with obesity.","authors":"Siresha Bathina, Virginia Fuenmayor Lopez, Mia Prado, Elliot Ballato, Georgia Colleluori, Maryam Tetlay, Dennis Tan Villareal, Sanjay Mediwala, Rui Chen, Clifford Qualls, Reina Armamento-Villareal","doi":"10.14814/phy2.70124","DOIUrl":"10.14814/phy2.70124","url":null,"abstract":"Growth differentiation factor (GDF15) has been considered a biomarker and recently a hormonal driver for diseases in different populations. However, the role of GDF15 as a biomarker of health outcomes in obese men from different racial/ethnic background has not been evaluated. The objective of this study was to investigate the racial/ethnic differences on the relationship between GDF15 and markers of glucometabolic status, hormonal profile, body composition and bone mineral density (BMD) in obese men. One hundred ninety-three obese men from diverse racial/ethnic backgrounds were enrolled. BMD and body composition were measured by dual energy X-ray absorptiometry. Serum GDF15, osteocalcin, C-terminal telopeptide, sclerostin, adiponectin, leptin, estradiol, testosterone, follicle-stimulating hormone, luteinizing hormone, 25-hydroxyvitamin D, lipid profile, and hemoglobin A1C (A1C) were measured. Non-African Americans (NAA) had significantly higher GDF15 level than African Americans (AA). Level was also higher in patients with type 2 diabetes (T2DM). In both the groups GDF15 correlated with A1C and lean mass. However. GDF15 correlated with body fat, LDL total cholesterol and femoral neck BMD only in NAA and with appendicular lean mass only in AA. Ethnicity, total cholesterol and T2DM were found to be independent predictors of GDF15. We conclude that GDF15 may influence glucometabolic status, body composition and bone parameters which may affect cardiovascular risk and osteoporosis between races.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70124"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0