Physiological Reports最新文献_第5页

Trans-right ventricle metabolite gradients in obesity highlight multiple metabolic pathways. 肥胖的右心室代谢物梯度突出了多种代谢途径。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70323

Hanna M Knauss, Mythri Ambatipudi, Leah B Kosyakovsky, Matthew S Herzig, Jessica K Wang, Elizabeth E Liu, Emily S Lau, Jenna N McNeill, Xu Shi, Robert E Gerszten, Naomi M Hamburg, Gregory D Lewis, Jeremy M Robbins, Jennifer E Ho

{"title":"Trans-right ventricle metabolite gradients in obesity highlight multiple metabolic pathways.","authors":"Hanna M Knauss, Mythri Ambatipudi, Leah B Kosyakovsky, Matthew S Herzig, Jessica K Wang, Elizabeth E Liu, Emily S Lau, Jenna N McNeill, Xu Shi, Robert E Gerszten, Naomi M Hamburg, Gregory D Lewis, Jeremy M Robbins, Jennifer E Ho","doi":"10.14814/phy2.70323","DOIUrl":"https://doi.org/10.14814/phy2.70323","url":null,"abstract":"Obesity and metabolic dysfunction are associated with pulmonary vascular remodeling, yet molecular mechanisms remain poorly understood. We sought to study trans-right ventricular (RV) metabolite gradients to elucidate potential molecular pathways operant among individuals with obesity and pulmonary hypertension. In this study, 38 individuals with obesity (mean age 58 years, 68% women, average BMI 36.6 kg/m2) underwent invasive right heart catheterization. Multi-site blood sampling from the superior vena cava and pulmonary artery was performed to assess trans-RV gradients, with targeted metabolite profiling using liquid chromatography-mass spectrometry. We found 56 metabolites with significant trans-RV gradients (FDR q < 0.05), including intermediates of fatty acid oxidation, the tricarboxylic acid cycle, and nucleotide metabolism. Further, trans-RV gradients in lipid and purine metabolism were associated with BMI and related cardiometabolic traits, such as waist circumference, insulin resistance, and serum lipids. Finally, differential levels of bile acids, intermediates of lipid peroxidation, and nucleotide metabolism across the RV were associated with pulmonary hypertension. In conclusion, trans-RV metabolite gradients among individuals with obesity reveal alterations in metabolites representative of molecular pathways such as fatty acid oxidation, and others correlated with cardiometabolic traits and/or pulmonary hypertension, including orotic acid, bile acids, and acylcarnitines.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70323"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association of mitochondrial morphology and supercomplex redistribution with skeletal muscle oxidative capacity in older adults. 老年人线粒体形态和超复合体再分布与骨骼肌氧化能力的关系。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70359

Mauricio Castro Sepulveda, Sylviane Lagarrigue, Francesca Amati

{"title":"The association of mitochondrial morphology and supercomplex redistribution with skeletal muscle oxidative capacity in older adults.","authors":"Mauricio Castro Sepulveda, Sylviane Lagarrigue, Francesca Amati","doi":"10.14814/phy2.70359","DOIUrl":"https://doi.org/10.14814/phy2.70359","url":null,"abstract":"Skeletal muscle maximal oxidative capacity (ATPmax) is a key component of age-related sarcopenia and muscle health. The contribution of mitochondrial morphology and electron transport chain supercomplex (SC) assemblies to ATPmax has yet to be determined in human muscle. ATPmax measured in vivo by 31phosphorus magnetic resonance spectroscopy in the quadriceps femoris of nine volunteers (65.5 ± 3.3 years old) was correlated with muscle biopsy outcomes before and after 4 months of supervised exercise. Mitochondrial morphology was assessed in electron micrographs, and SCs were measured by blue native gel electrophoresis. In the sedentary conditions, ATPmax was positively associated with complex (C) I and CIII in SC I+III2+IVn and negatively associated with CI and CIII in SC I+III2. Regarding mitochondrial morphology, ATPmax was positively associated with markers of mitochondrial elongation. Exercise training-induced increases in ATPmax were accompanied by mitochondrial elongation and by the redistribution of free complex III. Indicators of mitochondrial elongation were associated with the redistribution of specific complexes to SC I+III2+IVn. Higher skeletal muscle oxidative capacity in older adults is associated with mitochondrial elongation and the redistribution of electron transport chain complexes into higher rank SCs in the same muscle. Further, we provide evidence that mitochondrial elongation favors mitochondrial SC assembly.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70359"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ventilatory efficiency in cardiac amyloidosis-A systematic review and meta-analysis. 心脏淀粉样变性患者的通气效率：系统评价和荟萃分析。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70308

Robin Willixhofer, Elisabetta Salvioni, Nicolò Capra, Mauro Contini, Jeness Campodonico, Piergiuseppe Agostoni

{"title":"Ventilatory efficiency in cardiac amyloidosis-A systematic review and meta-analysis.","authors":"Robin Willixhofer, Elisabetta Salvioni, Nicolò Capra, Mauro Contini, Jeness Campodonico, Piergiuseppe Agostoni","doi":"10.14814/phy2.70308","DOIUrl":"https://doi.org/10.14814/phy2.70308","url":null,"abstract":"In cardiac amyloidosis (CA) cardiopulmonary exercise testing (CPET) is underexplored. This study evaluated exercise limitations in CA using CPET, focusing on the ventilation-to-carbon dioxide production (VE/VCO2) slope and peak oxygen uptake (VO2). Seventeen studies involving 1505 patients were analyzed and systematically reviewed according to PRISMA reporting guidelines. Subgroup analyses assessed differences by diagnosis (ATTR vs. AL), CPET modality, and age. The cohort included 12% with AL, 80% with ATTR (23% hereditary [ATTRv], 70% wild-type [ATTRwt], 7% unspecified), and 8% unidentified subtypes. VE/VCO2 slope was elevated across ATTR subgroups: 38.4 (95% CI: 36.9-40.0, I2 = 57%) in ATTRwt and 37.9 (95% CI: 35.1-40.7, I2 = 70%) in ATTRv. ATTR patients were older than AL patients by 9.0 years (95% CI: 0.4-17.6, I2 = 88%) and had a higher VE/VCO2 slope: 2.5 (95% CI: 0.2-4.8, I2 = 0%). CPET modality influenced peak VO2, which was lowest for treadmill exercise (13.7, 95% CI: 12.7-14.8, I2 = 0%, mL/min/kg) compared to upright cycle ergometry (14.7, 95% CI: 14.3-15.1, I2 = 33%) and semi-recumbent cycle ergometry (14.5, 95% CI: 14.1-14.9, I2 = 28%). A high VE/VCO2 slope characterizes both ATTRwt and ATTRv, while AL patients are younger with lower VE/VCO2 slope levels. Peak VO2 in ATTR patients may depend on exercise modality.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70308"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a competitive season on the plasma lipid profile of soccer players. 竞技赛季对足球运动员血脂的影响。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70382

Santo Marsigliante, Giulia My, Okba Selmi, Enrico Paolo Villani, Aymen Hawani, Antonella Muscella

{"title":"Effects of a competitive season on the plasma lipid profile of soccer players.","authors":"Santo Marsigliante, Giulia My, Okba Selmi, Enrico Paolo Villani, Aymen Hawani, Antonella Muscella","doi":"10.14814/phy2.70382","DOIUrl":"10.14814/phy2.70382","url":null,"abstract":"This study aimed to evaluate the impact of a soccer season on the lipid profile of professional soccer players. Forty male players participated in the study. Blood samples for lipid profile analysis were collected at four time points: before the start of the training period (T0), immediately after the 4-week training phase (T1), at mid-season (T2, 23 weeks after T0), and the end of the season (T3, 43 weeks after T0). Throughout the competitive season, there was a significant decrease in body fat percentage (BFP, p < 0.001, RM-ANOVA) and an increase in fat-free mass (FFM, p < 0.001, RM-ANOVA). Plasma levels of total cholesterol (TC) and LDL cholesterol (LDL) significantly decreased (TC: p = 0.003, LDL: p = 0.033, RM-ANOVA), whereas HDL cholesterol (HDL) levels increased significantly in response to training (p = 0.013, RM-ANOVA). Triglyceride (TG) concentrations were significantly higher before the season than after (p < 0.001, RM-ANOVA). Additionally, the LDL/HDL and TC/HDL ratios showed significant reductions over the season (LDL/HDL: p = 0.004, TC/HDL: p < 0.001, RM-ANOVA). The observed decrease in LDL, TC, and TG levels, along with the increase in HDL levels at the end of the season. These results suggest that regular soccer training induces favorable changes in the lipid profile of professional players. Further studies are needed to determine whether such changes are associated with long-term health outcomes in elite athletes, particularly considering their typically low baseline risk for cardiovascular conditions.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 10","pages":"e70382"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of TFEB in human placental Cytotrophoblasts and Syncytiotrophoblasts. TFEB在人胎盘细胞滋养细胞和合胞滋养细胞中的调控作用。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70383

A Mathew, J S Trausch-Azar, C Azar, M Schuetz, M R Mahjoub, A L Schwartz

{"title":"Regulation of TFEB in human placental Cytotrophoblasts and Syncytiotrophoblasts.","authors":"A Mathew, J S Trausch-Azar, C Azar, M Schuetz, M R Mahjoub, A L Schwartz","doi":"10.14814/phy2.70383","DOIUrl":"10.14814/phy2.70383","url":null,"abstract":"While cellular proteins exist in a dynamic state maintained by the balance of synthesis and degradation, there is a paucity of information on these processes in placental trophoblasts, including within cytotrophoblasts which differentiate into multi-nucleate syncytiotrophoblasts. TFEB, a transcription factor with a myriad of cellular activities, is one of the most abundant genes expressed in syncytiotrophoblasts compared to cytotrophoblasts. TFEB is localized to the nucleus of human BeWo differentiated syncytiotrophoblasts and to the cytoplasm of the undifferentiated cytotrophoblasts. Within both the cytotrophoblasts and syncytiotrophoblasts, TFEB exists in subcellular compartments as both phosphorylated and unphosphorylated forms and translocates between cytoplasm and nucleus upon amino acid starvation/refeeding. Endogenous TFEB and endogenous phospho-TFEB are both rapidly (t1/2 ~ 2-3 h) degraded via the ubiquitin proteasome system in cytotrophoblasts and in syncytiotrophoblasts. These results suggest dynamic regulatory processes during trophoblast development/differentiation.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 10","pages":"e70383"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Threshold estimation in running using dynamical correlations of RR intervals. 基于RR区间动态相关性的运行阈值估计。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70241

Matias Kanniainen, Vesa Laatikainen-Raussi, Teemu Pukkila, Krista Vohlakari, Esa Hynynen, Johanna K Ihalainen, Esa Räsänen

{"title":"Threshold estimation in running using dynamical correlations of RR intervals.","authors":"Matias Kanniainen, Vesa Laatikainen-Raussi, Teemu Pukkila, Krista Vohlakari, Esa Hynynen, Johanna K Ihalainen, Esa Räsänen","doi":"10.14814/phy2.70241","DOIUrl":"https://doi.org/10.14814/phy2.70241","url":null,"abstract":"We study the estimation of aerobic threshold (AeT) and anaerobic threshold (AnT) using dynamical detrended fluctuation analysis (DDFA). Conventionally, the thresholds are estimated in laboratory settings, where the subject performs an incremental exercise test on a cycloergometer or treadmill. We compared DDFA-based thresholds (DDFAT1 and DDFAT2) with lactate thresholds (LT1 and LT2) and examined thresholds derived from theoretical and measured maximal heart rates (HR). The analysis was conducted on 58 subjects undergoing an incremental treadmill running test. Our findings indicate significant discrepancies between thresholds derived from theoretical and measured maximal HRs compared to lactate thresholds. Specifically, theoretical maximal HR thresholds consistently underestimated lactate thresholds, exhibiting systematic bias. Measured maximal HR thresholds also showed a consistent underestimation, though with improved alignment to lactate thresholds. In contrast, the DDFA-based method demonstrated reasonable agreement with lactate thresholds and lacked systematic bias. The DDFA-based approach offers a simple and accurate alternative for estimating AeT and AnT. Its potential for continuous monitoring makes it suitable for integration into wearable devices such as smartwatches and heart rate monitors.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70241"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic stress elicits sex-specific mitochondrial respiratory functional changes in the rat heart. 慢性应激引起大鼠心脏性别特异性线粒体呼吸功能改变。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70371

Caitlin P Odendaal-Gambrell, Cassidy O'Brien, Megan Cairns, Gerald J Maarman, Danzil E Joseph, Carine Smith, Fanie Rautenbach, Jeanine L Marnewick, M Faadiel Essop

{"title":"Chronic stress elicits sex-specific mitochondrial respiratory functional changes in the rat heart.","authors":"Caitlin P Odendaal-Gambrell, Cassidy O'Brien, Megan Cairns, Gerald J Maarman, Danzil E Joseph, Carine Smith, Fanie Rautenbach, Jeanine L Marnewick, M Faadiel Essop","doi":"10.14814/phy2.70371","DOIUrl":"10.14814/phy2.70371","url":null,"abstract":"Although chronic psychosocial stress is linked to cardiovascular diseases, the underlying mechanisms remain elusive. For this study, we focused on the mitochondrion as a putative mediator of stress-related cardiac pathologies in a sex-dependent manner. Male and female Wistar rats were subjected to chronic stress for 4 weeks (mimicking an anxious phenotype) versus matched controls. Cardiac redox status, mitochondrial respiration parameters, and expression levels of proteins involved in mitochondrial oxidative phosphorylation, dynamics, and biogenesis were evaluated. Despite limited changes in behavior and circulating stress hormones (both sexes), stressed males exhibited altered cardiac oxidative phosphorylation via β-oxidation- and glucose oxidation-linked respiratory pathways together with increased myocardial antioxidant capacity and decreased lipid peroxidation. Conversely, stressed females exhibited a protective and resilient phenotype by displaying augmented levels of major mitochondrial respiratory complexes (complex I, III, and ATP synthase) and a fusion marker (mitofusin-2 [Mfn2]), together with attenuated expression of a fission marker (dynamin-related protein-1 [Drp1]) despite decreased estradiol levels. In contrast, stressed males displayed increased cardiac ATP synthase levels together with diminished peroxisome proliferator-activated receptor-gamma coactivator-1-alpha (PGC-1α) expression versus controls. These findings indicate that male mitochondria are more prone to stress-related functional changes, while females exhibited a more protective and resilient phenotype.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70371"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers. 在机器灌注过程中，治疗性热疗促进肝脏脂质输出和HSP70/90。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70348

Adam M Thorne, Yana Geng, Veerle A Lantinga, Marieke Smit, Jan Albert Kuivenhoven, Robert J Porte, Folkert Kuipers, Peter Olinga, Justina C Wolters, Vincent E de Meijer

{"title":"Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers.","authors":"Adam M Thorne, Yana Geng, Veerle A Lantinga, Marieke Smit, Jan Albert Kuivenhoven, Robert J Porte, Folkert Kuipers, Peter Olinga, Justina C Wolters, Vincent E de Meijer","doi":"10.14814/phy2.70348","DOIUrl":"https://doi.org/10.14814/phy2.70348","url":null,"abstract":"Liver transplantation is the only curative option for end-stage liver disease. Donor shortages necessitate the use of higher risk donor livers, including fatty livers, which are more susceptible to ischemia-reperfusion injury. Machine perfusion has improved graft utilization and is typically performed at hypothermic (8-12°C) or normothermic (35-37°C) temperatures. Here we studied the impact of mild hyperthermia (40°C) as a therapeutic intervention for fatty livers using in-depth proteomic and lipoprotein profiling of whole organ perfusion and precision-cut liver slices. We observed proteomic changes with metabolic alterations over time, evidenced by a significant increase in lipid export in whole organ perfusions. Furthermore, PCLS showed significant upregulation of metabolic processes and heat shock protein response after 24 h of hyperthermia. Machine perfusion under hyperthermic conditions may be a potential strategy to improve the utilization of fatty liver grafts, ultimately expanding the donor pool and improving transplant outcomes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70348"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood buffers: The viewpoint of a biochemist. 血液缓冲液：生物化学家的观点。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70333

Andrea Bellelli

{"title":"Blood buffers: The viewpoint of a biochemist.","authors":"Andrea Bellelli","doi":"10.14814/phy2.70333","DOIUrl":"https://doi.org/10.14814/phy2.70333","url":null,"abstract":"Mammalian blood is a very complex system whose multiple physiological roles require that its pH is maintained constant, in spite of the necessity of carrying over 15 moles of CO2 a day from the tissues to the lungs. The blood pH is maintained constant by several buffers, whose interplay is complex. The study of blood buffers is over a century old and has crossed major reinterpretations of the nature of acids and bases, from Arrhenius to Bronsted and Lowry, as well as an enormous evolution of our knowledge of protein structure, proteins being the most relevant among blood buffers. As a consequence, several theories have been developed to explain the physiological and pathological fluctuations of blood pH. This review compares the three main theories currently used: that based on the Strong Ions Difference (SID), due to Stewart and his followers; that based on the Base Excess, due to the Copenhagen school of respiration physiology; and the physiological one, due to the Boston school. These theories are not alternative but complementary and can be reconciled with each other, provided that some erroneous assumptions are corrected, and all three are expressed using the definitions proposed by Bronsted and Lowry.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70333"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Void spot assay visualization optimization for use of Void Whizzard in rats (Rattus norvegicus). Void wizard在褐家鼠（Rattus norvegicus）中使用的空洞斑点实验可视化优化。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70358

Hannah Ruetten, Jadyn Bothe, Shannon Lankford, Gopal Badlani, James Koudy Williams

{"title":"Void spot assay visualization optimization for use of Void Whizzard in rats (Rattus norvegicus).","authors":"Hannah Ruetten, Jadyn Bothe, Shannon Lankford, Gopal Badlani, James Koudy Williams","doi":"10.14814/phy2.70358","DOIUrl":"https://doi.org/10.14814/phy2.70358","url":null,"abstract":"Void spot assay (VSA) noninvasively evaluates urination. This study optimizes VSA by comparing post-assay paper visualization techniques: bright field light (BF), ultraviolet light (UV), and ninhydrin spray (N). Male rats were placed in filter paper lined cages for 4 h. After the assay, all papers were dried. BF images were photographed (digital camera). UV images were captured using a Darkroom ultraviolet imaging cabinet. Papers were sprayed with ninhydrin and photographed (digital camera). All images were converted to binary for analysis with Void Whizzard. UV versus BF significantly differed in area. All three groups significantly differed in overall spot count and spots 0-0.1 cm2. UV versus N and UV versus BF significantly differed in 0.1-0.25 cm2 spots, UV versus N in 0.25-0.5 cm2, and N versus BF in spots 0.5-1 cm2. Overall BF visualization proved difficult. N provided an ideal way to highlight urine and image with a digital camera. Human fingerprints from pre-assay handling of paper interfered with the analysis of the smallest sized spots; however, there were no differences in the detection of larger spots, spot distribution, or overall spot area. This study contributes to the development of a standardized VSA protocol for assessing bladder function in rodent models.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70358"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0