Peptide research最新文献_第8页

Hydrophobic interactions and the design of receptor mimetic peptides. 疏水相互作用和受体模拟肽的设计。

Peptide research Pub Date : 1995-03-01

S A Martin-Moe, R Lehr, M D Cauley, G R Moe

{"title":"Hydrophobic interactions and the design of receptor mimetic peptides.","authors":"S A Martin-Moe, R Lehr, M D Cauley, G R Moe","doi":"","DOIUrl":"","url":null,"abstract":"The possibility that hydrophobic interactions may be used as a basis for the design of receptor mimetic peptides for small peptide hormones that lack the potential to adopt amphiphilic secondary structures was tested by designing and characterizing receptor mimetic peptides for gamma-endorphin. The receptor mimetic peptides were designed to exhibit a pattern of hydrophobic surfaces in an antiparallel orientation matching that of the peptide hormone in an extended conformation. An ELISA-based assay was used to determine the relative binding affinities of receptor mimetic peptides, control peptides and antisense peptides to gamma-endorphin immobilized on a surface. The inhibition constant for the best gamma-endorphin receptor mimetic peptide was 1.6 microM. No binding was detected for scrambled control peptides or the antisense-derived peptide mimetic to the limit of their respective solubilities. Sera from rabbits immunized with a gamma-endorphin receptor mimetic peptide were used to immunopurify the ligand-binding domain of the human opiate receptor and were cross-reactive with purified bovine opiate receptor. These results suggest that patterns of hydrophobicity can provide a rational basis for designing receptor mimetic peptides and may provide an explanation for the ability of some antisense peptides to bind to their cognate hormones and to elicit antibodies cross-reactive with hormone receptors.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 2","pages":"70-6"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18552770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient eukaryotic gene expression vectors for peptide secretion. 高效的肽分泌真核基因表达载体。

Peptide research Pub Date : 1995-03-01

T H Chu, I Martinez, P Olson, R Dornburg

{"title":"Highly efficient eukaryotic gene expression vectors for peptide secretion.","authors":"T H Chu, I Martinez, P Olson, R Dornburg","doi":"","DOIUrl":"","url":null,"abstract":"Recently, we constructed a series of highly efficient universal eukaryotic gene expression vectors (Sheay et al., BioTechniques 15:856-862, 1993). Such vectors contain a viral promoter and enhancer followed by the adenovirus tripartite leader sequence, a multiple cloning site for the insertion of the gene of interest and a polyadenylation sequence. To enable expression of peptides to be secreted into the tissue culture medium or to be incorporated into the cell membrane, several modifications have been introduced into such vectors: stop codons in all three reading frames were inserted at the end of the multiple cloning site and a DNA sequence coding for a signal peptide for transport through the endoplasmatic reticulum (ER) was introduced downstream of the adenovirus tripartite leader sequence followed by two unique restriction enzyme recognition sites. A protocol is described that allows fast and easy cloning of peptide-coding regions, i.e., PCR products, for expression and secretion. The transport of a genetically engineered chimeric transmembrane protein connected to this ER leader sequence was as efficient as that of the original protein from which the ER sequence has been derived. These universal vectors can also be used for the easy construction of any chimeric transmembrane or secretion proteins.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 2","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18658026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuropeptide Y receptor distribution and regulation in the suprachiasmatic nucleus of the Syrian hamster (Mesocricetus auratus). 神经肽Y受体在叙利亚仓鼠视交叉上核的分布和调控。

Peptide research Pub Date : 1995-03-01

E G Stopa, J K Johnson, D I Friedman, H I Ryer, J Reidy, V Kuo-LeBlanc, H E Albers

{"title":"Neuropeptide Y receptor distribution and regulation in the suprachiasmatic nucleus of the Syrian hamster (Mesocricetus auratus).","authors":"E G Stopa, J K Johnson, D I Friedman, H I Ryer, J Reidy, V Kuo-LeBlanc, H E Albers","doi":"","DOIUrl":"","url":null,"abstract":"The suprachiasmatic nucleus (SCN) receives a direct photic projection from the retina, the retinohypothalamic tract (RHT), and an indirect photic projection from the intergeniculate leaflet of the thalamus, the geniculohypothalamic tract (GHT). The primary neurochemical signal in the GHT appears to be neuropeptide Y (NPY), and several lines of evidence indicate that NPY may be involved in determining the response of the SCN to light. The purpose of the present study was (i) to localize NPY binding sites in the hamster SCN and to compare the distribution of these binding sites with the terminal field of the RHT and (ii) to determine if SCN levels of NPY binding change during the day. RHT fibers, defined using the carbocyanine dye DiI, were localized primarily within the ventrolateral region of the SCN. The distribution of NPY receptors, as visualized by 125I-peptide YY (PYY) binding, overlapped the projection field of the RHT. Specific binding of 125I-PYY was significantly greater in the ventrolateral SCN than in the medial SCN. To determine whether NPY binding changes during the day, the levels of 125I-PYY in the SCN were determined 4 h before the onset of darkness, 1 h before the onset of darkness and 2 h after the onset of darkness in hamsters housed in a 14:10 light-dark cycle. The levels of binding at 4 and 1 h before dark onset were significantly lower than 2 h after the onset of darkness. In contrast, no significant differences were observed in 125I-PYY binding over these same sampling intervals in either the medial preoptic area or the lateral hypothalamus.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 2","pages":"95-100"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18659039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence-specific 1H NMR assignments and secondary structure of a lipid-associating peptide from human ApoC-I: an NMR study of an amphipathic helix motif. 人类apoc - 1脂质相关肽的序列特异性1H NMR分配和二级结构:两亲螺旋基序的NMR研究。

Peptide research Pub Date : 1995-03-01

G W Buchko, A Rozek, Q Zhong, R J Cushley

引用次数: 0

Determination of enantiomers of histidine, arginine and other amino acids by HPLC of their diastereomeric N-ethoxycarbonyldipeptides. 用高效液相色谱法测定组氨酸、精氨酸和其他氨基酸的非对映体n -乙氧羰基二肽。

Peptide research Pub Date : 1995-03-01

N L Benoiton, Y C Lee, R Steinauer

引用次数: 0

Peptide ionophores: synthesis and cation-binding properties of a bicyclic peptide containing glycine and lysine residues. 肽离子载体:含有甘氨酸和赖氨酸残基的双环肽的合成和阳离子结合特性。

Peptide research Pub Date : 1995-03-01

E Crusi, E Giralt, D Andreu

引用次数: 0

Preparation and evaluation of PEG-bound thrombin inhibitors based on 4-amidinophenylalanine. 基于4-氨基苯丙氨酸的聚乙二醇结合凝血酶抑制剂的制备与评价。

Peptide research Pub Date : 1995-03-01

W Stüber, R Koschinsky, M Reers, D Hoffmann, J Czech, G Dickneite

引用次数: 0

Quantification of cysteinyl sulfhydryl residues in peptides and proteins by ESI-MS or MALDI-MS. ESI-MS或MALDI-MS定量测定多肽和蛋白质中半胱氨酸巯基残基。

Peptide research Pub Date : 1995-03-01

D Ming, J L Markley, G Hellekant

引用次数: 0

Larger scale multipin peptide synthesis. 大规模多针肽合成。

Peptide research Pub Date : 1995-01-01

N J Maeji, A M Bray, R M Valerio, W Wang

引用次数: 0

Novel pseudopeptides with high affinities for the human bradykinin B2 receptor. 与人缓激素B2受体具有高亲和力的新型假多肽。

Peptide research Pub Date : 1995-01-01

S Chakravarty, M Connolly, D J Kyle

{"title":"Novel pseudopeptides with high affinities for the human bradykinin B2 receptor.","authors":"S Chakravarty, M Connolly, D J Kyle","doi":"","DOIUrl":"","url":null,"abstract":"We recently proposed a model of bradykinin bound to the rat bradykinin B2 receptor that is constructed on the basis of structural homology modeling to the criomicroscopic structure of the seven transmembrane domains of bacteriorhodopsin, extensive conformational searches and experimental mutagenesis results. On the basis of that model, a novel third-generation pseudopeptide antagonist, NPC 18325 (D-Arg1-Arg2-[aminotridecanoyl]3-Ser4-D-Tic5-Oic6++ +-Arg7) (Ki = 440 nM, guinea pig ileum), was designed and also reported. NPC 18325 has been proposed to adopt a C-terminal beta turn separated from N-terminal positive charges by a linear 12 carbon chain spacer. Experimentally, the four amino acids making up the C-terminus have been shown by NMR to preferentially adopt a beta turn at neutral pH in aqueous solution. We now present a series of peptides, related to and including NPC 18325, that explore the relationship between the length of the carbon chain and the affinity to the human bradykinin B2 receptor. The results show that there is a structure-activity relationship (SAR) associated with the chain length and that these pseudopeptides have better affinity to the human bradykinin receptor than they have to the guinea pig ileal-derived B2 receptor. Specifically, peptide I (a 12-methylene linker) had a measured Ki of 31 nM and peptide V (a 4-methylene linker) had a Ki of 471 nM. Implications regarding conformation and hydrophobicity are also described.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 1","pages":"16-9"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18757573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0