Peptide research最新文献_第10页

High yield, directed immobilization of a peptide-ligand onto a beaded cellulose support. 高产、定向固定化肽配体到珠状纤维素载体上。

Peptide research Pub Date : 1994-11-01

D R Englebretsen, D R Harding

引用次数: 0

A general approach for identifying and cloning peptide synthetase genes. 鉴定和克隆肽合成酶基因的一般方法。

Peptide research Pub Date : 1994-09-01

K Turgay, M A Marahiel

引用次数: 0

Preliminary experimental anticancer activity of cecropins. 天蚕素抗癌活性的初步实验研究。

Peptide research Pub Date : 1994-09-01

A J Moore, D A Devine, M C Bibby

引用次数: 0

Sequential order of T and B cell epitopes affects immunogenicity but not antibody recognition of the B cell epitope. T和B细胞表位的顺序影响免疫原性，但不影响B细胞表位的抗体识别。

Peptide research Pub Date : 1994-09-01

G Denton, F Hudecz, J Kajtár, A Murray, S J Tendler, M R Price

{"title":"Sequential order of T and B cell epitopes affects immunogenicity but not antibody recognition of the B cell epitope.","authors":"G Denton, F Hudecz, J Kajtár, A Murray, S J Tendler, M R Price","doi":"","DOIUrl":"","url":null,"abstract":"Synthetic peptide constructs, co-linearly linking a MUC1 mucin B cell epitope peptide to a known murine T cell epitope, both in T-B and B-T orientations, show that induction of high murine anti-MUC1 antibody titers is dependent on the presence and orientation of the T cell determinant. However, the sequential order of the epitopes does not affect binding of anti-B cell epitope antibodies to the constructs. Haplotype mismatching leads to a significant lowering of the anti-MUC1 antibody responses, implicating a central role for the T cell epitope in eliciting anti-B cell epitope responses. Secondary structure analysis by circular dichroism spectroscopy reveals the T-B construct to be partially ordered, while the B-T peptide adopts a highly ordered conformation in trifluoroethanol. These studies suggest that the sequential order of epitopes may significantly alter the immunogenicity of the peptide but may not necessarily affect its antigenicity. Immunogenicity of the peptide constructs may be governed by subtle differences in secondary structure, leading to variation in the way peptides are presented or processed within cells governing immune responses. These findings have relevance for the construction of peptides to be utilized as potential synthetic vaccines and for the design of peptide immunogens.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 5","pages":"258-64"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18540742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a linear pentapeptide containing two consecutive beta-turns. 一个线性五肽的特性，包含两个连续的-转。

Peptide research Pub Date : 1994-09-01

K Ramnarayan, V N Balaji, K I Varughese

{"title":"Characterization of a linear pentapeptide containing two consecutive beta-turns.","authors":"K Ramnarayan, V N Balaji, K I Varughese","doi":"","DOIUrl":"","url":null,"abstract":"The contiguous occurrence of two beta-turns is examined using molecular mechanics calculations. A tripeptide can take up special conformations known as beta-turns resulting in the reversal of the chain. There are two major classes of beta-turns, called type I beta-turn and type II beta-turn. In the specific case described here, the third peptide unit forms a part of a second turn resulting in the formation of a two-turn motif. In the case of dihydropteridine reductase, this motif is involved in cofactor binding. This study examines the energetic and conformational preferences for chain-reversed motifs. Energy minimizations were carried out on models of pentapeptides with four different sequences for residues 2 through 5: (i) GGGG, (ii) AGGA, (iii) AGAG and (iv) AAAA. For each of the above sequences, all four possible combinations of type I and type II beta-turns were considered. Out of the four possible combinations, the (II, II) combination is the most planar one. The (I, I) combination is the least planar. For the all-Gly model and the all-Ala model, the most favored conformation energetically is a type I-type I combination. On the other hand, the sequence AGGA favors a type II-type I combination, and the sequence AGAG prefers a type II-type II combination. A computer search for double-turn motifs at the Brook-haven Protein Data Bank revealed that the (I, I) combination occurs with the highest frequency, and the (I, II) combination has the next highest frequency.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 5","pages":"270-8"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A practical synthesis of optically pure and fully protected L-gamma-carboxyglutamic acid derivatives and its application in peptide synthesis. 光纯和全保护的l - γ -羧基谷氨酸衍生物的实际合成及其在肽合成中的应用。

Peptide research Pub Date : 1994-09-01

P T Ho, K Ngu

引用次数: 0

Primary structure of a novel human salivary acidic proline-rich protein. 一种新型人唾液酸性富含脯氨酸蛋白的初级结构。

Peptide research Pub Date : 1994-09-01

D H Schlesinger, D I Hay, S K Schluckebier, J M Ahern

{"title":"Primary structure of a novel human salivary acidic proline-rich protein.","authors":"D H Schlesinger, D I Hay, S K Schluckebier, J M Ahern","doi":"","DOIUrl":"","url":null,"abstract":"Human salivary acidic proline-rich proteins (PRPs) form a significant fraction of the total salivary protein and fulfill several biologically important roles in the oral cavity. Five commonly occurring PRP polymorphisms, Db, Pa, PIF, Pr2 and Pr1, have been identified, their structures determined, and several uncommon polymorphisms (frequencies < 1:100) have been reported. Most PRPs occur as protein pairs, because of an unusual, limited but well-controlled post-translational cleavage. We now describe an additional uncommon polymorphism, found in the saliva of one of 127 individuals examined in a recent study, identified by high performance anion-exchange liquid chromatography. By analogy with previous terminology, we designate this protein pair as PRP-5, for the primary 150-residue polypeptide gene product, and PRP-6, for the secondary 106-residue cleavage product. Amino acid analysis of intact PRP-6 and sequence determination of PRP-6 chymotryptic peptides, residues 15-24 and 26-35, show a single difference in PRP-6, compared to the most similar, characterized PRP, PRP-4, in that residue 30 is histidine in PRP-6, rather than arginine as in PRP-4 and in all the other sequenced PRPs. This substitution may have implications for the resistance of this polymorphic variant to degradation by trypsin-like enzymes originating from the oral microflora.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 5","pages":"242-7"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18848907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting antigenic determinants in proteins: looking for unidimensional solutions to a three-dimensional problem? 预测蛋白质中的抗原决定因素:寻找三维问题的一维解决方案?

Peptide research Pub Date : 1994-07-01

M H Van Regenmortel, J L Pellequer

{"title":"Predicting antigenic determinants in proteins: looking for unidimensional solutions to a three-dimensional problem?","authors":"M H Van Regenmortel, J L Pellequer","doi":"","DOIUrl":"","url":null,"abstract":"In a recent review, Hopp (Peptide Research 6:183-190, 1993) claimed that the Hopp and Woods hydrophilicity method for locating antigenic determinants is superior to all other existing methods for predicting the B cell epitopes of proteins but that it is not useful to aid the investigator in producing peptide-protein cross-reactive antisera. In this article, we challenge both these assertions. Most investigators utilize antigenicity prediction algorithms because they wish to produce anti-peptide antibodies capable of cross-reacting with the intact protein. All prediction methods are based on propensity scales for the 20 amino acids, which describe the tendency of each residue to be associated with properties such as hydrophilicity, surface accessibility or segmental mobility. When we compared the prediction efficacy of 22 different scales, taking into account both correct and incorrect predictions, we found that none of the scales gave a level of correct prediction higher than about 50%-60%. If no antigenicity was found in a particular region of the protein, we took the view that hydrophilicity peaks located in that region amounted to wrong predictions. The much higher success rate reported by Hopp for this method stems from the way he assesses prediction efficacy, i.e., by counting the number of known epitopes located inside and outside hydrophilicity peaks. Reasons for the low success rate of antigenicity prediction are discussed. In most cases, it is unrealistic to try to reduce the complexity of discontinuous, conformational epitopes to simple, linear peptide models.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 4","pages":"224-8"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18542541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different views of protein antigenicity. 对蛋白质抗原性的不同看法。

Peptide research Pub Date : 1994-07-01

T P Hopp

引用次数: 0

Influence of solid support, solvent and coupling reagent on the head-to-tail cyclization of resin-bound peptides. 固体载体、溶剂和偶联剂对树脂结合肽首尾环化的影响。

Peptide research Pub Date : 1994-07-01

J S McMurray, C A Lewis, N U Obeyesekere

引用次数: 0