Peptide research最新文献_第9页

Development of GM-CSF antagonist peptides. GM-CSF拮抗剂肽的开发。

Peptide research Pub Date : 1995-01-01

J M VonFeldt, C Monfardini, S Fish, H Rosenbaum, T Kieber-Emmons, R M Williams, S A Khan, D B Weiner, W V Williams

{"title":"Development of GM-CSF antagonist peptides.","authors":"J M VonFeldt, C Monfardini, S Fish, H Rosenbaum, T Kieber-Emmons, R M Williams, S A Khan, D B Weiner, W V Williams","doi":"","DOIUrl":"","url":null,"abstract":"Granulocyte/macrophage colony stimulating factor (GM-CSF) is both a hematopoietic growth factor and a cytokine implicated in inflammatory disease. The development of GM-CSF antagonist peptides corresponding to the GM-CSF native sequence should allow their modification into higher affinity analogs, but this is hampered by the low affinity of linear peptides. To adequately evaluate such low affinity peptides, the use of several independent assays should allow specific versus nonspecific inhibitors to be distinguished. In this study, inhibition of GM-CSF-dependent cell growth, inhibition of GM-CSF binding and immunologic cross-reactivity between GM-CSF-derived peptides and native protein by neutralizing antibodies have been used to evaluate peptide analogs with potential bioactivity. The GM-CSF sequence was divided into 6 peptides ranging in size from 15-24 amino acids. Antisera were raised to these peptides in mice and assayed for immunologic cross-reactivity. 4/6 anti-peptide antisera bound GM-CSF on ELISA and 3/6 on immunoprecipitation. Antisera to two of the peptides (corresponding to residues 17-31 and 96-112) inhibited GM-CSF-dependent cellular proliferation in two cell lines, with one peptide derived from residues 17-31 demonstrating inhibition of GM-CSF binding and direct biological inhibitory activity. A peptide that did not elicit native GM-CSF reactive antibodies, corresponding to residues 54-78, was recognized by two neutralizing monoclonal antibodies. It exhibited inhibition of GM-CSF binding and direct biological antagonist activity. These studies implicate two sites in mediating GM-CSF biological activity, and indicate that biological antagonists can be developed based on these sites.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 1","pages":"20-7, 30-2"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18757576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of TiCl4-mediated reduction of methionine sulfoxide in peptides with oxidizable or reducible residues. 含氧化残基或可还原残基肽中ticl4介导的蛋氨酸亚砜还原的评价。

Peptide research Pub Date : 1995-01-01

M W Pennington, M E Byrnes

引用次数: 0

Use of synthetic peptide libraries for the H-2Kd binding motif identification. 利用合成肽库鉴定H-2Kd结合基序。

Peptide research Pub Date : 1995-01-01

A Quesnel, A Casrouge, P Kourilsky, J P Abastado, Y Trudelle

{"title":"Use of synthetic peptide libraries for the H-2Kd binding motif identification.","authors":"A Quesnel, A Casrouge, P Kourilsky, J P Abastado, Y Trudelle","doi":"","DOIUrl":"","url":null,"abstract":"To identify Kd-binding peptides, an approach based on small peptide libraries has been developed. These peptide libraries correspond to all possible single-amino acid variants of a particular Kd-binding peptide, SYIPSAEYI, an analog of the Plasmodium berghei 252-260 antigenic peptide SYIPSAEKI. In the parent sequence, each position is replaced by all the genetically encoded amino acids (except cysteine). The multiple analog syntheses are performed either by the Divide Couple and Recombine method or by the Single Resin method and generate mixtures containing 19 peptides. The present report deals with the synthesis, the purification, the chemical characterization by amino acid analysis and electrospray mass spectrometry (ES-MS), and the application of such mixtures in binding tests with a soluble, functionally empty, single-chain H-2Kd molecule denoted SC-Kd. For each mixture, bound peptides were eluted and analyzed by sequencing. Since the binding tests were realized in noncompetitive conditions, our results show that a much broader set of peptides bind to Kd than expected from previous studies. This may be of practical importance when looking for low affinity peptides such as tumor peptides capable of eliciting protective immune response.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 1","pages":"44-51"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18758791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

(R)-isovaline homo-peptides adopt the left-handed 3(10)-helical structure. (R)-异缬氨酸同型肽采用左旋3(10)-螺旋结构。

Peptide research Pub Date : 1995-01-01

F Formaggio, M Crisma, G M Bonora, M Pantano, G Valle, C Toniolo, A Aubry, D Bayeul, J Kamphuis

{"title":"(R)-isovaline homo-peptides adopt the left-handed 3(10)-helical structure.","authors":"F Formaggio, M Crisma, G M Bonora, M Pantano, G Valle, C Toniolo, A Aubry, D Bayeul, J Kamphuis","doi":"","DOIUrl":"","url":null,"abstract":"A complete series of N- and C-blocked, monodispersed homo-oligopeptides from the sterically hindered (R)-isovaline residue to the hexamer level was synthesized step by step by solution methods and fully characterized. The preferred conformation of all the oligopeptides was determined in deuteriochloroform solution by Fourier transform infrared absorption and 1H nuclear magnetic resonance. In addition, the molecular structures of tripeptide, tetrapeptide and pentapeptide were assessed in the crystal state by x-ray diffraction. The results obtained confirm the conclusions from previous studies, namely that beta-bends and 3(10)-helices are preferentially adopted by isovaline-rich peptides, a clear indication that this C alpha, alpha-disubstituted glycine tends to induce folded structures much more extensively than its unmethylated parent compound alpha-aminobutyric acid. Furthermore, in this work we were able to demonstrate unambiguously for the first time that the relationship between isovaline chirality and helix screw sense is the same as that promoted by C alpha-monosubstituted glycines [(R)-amino acids give left-handed helical structures]. A comparison is also made with the conclusions extracted from published work on homo-oligopeptides from other C alpha-methylated amino acids.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"8 1","pages":"6-15"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18758790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of an N-3-guanidinopropylglycine (Narg) derivative as a versatile building block for solid-phase peptide and peptoid synthesis. n -3-胍基丙基甘氨酸(Narg)衍生物的合成，作为固相肽和类肽合成的通用构建块。

Peptide research Pub Date : 1994-11-01

G Heizmann, E R Felder

引用次数: 0

Synthesis of combinatorial libraries with only one representation of each structure. 每个结构只有一个表示的组合库的综合。

Peptide research Pub Date : 1994-11-01

M Stanková, S Wade, K S Lam, M Lebl

引用次数: 0

Cyclic peptide template combinatorial libraries: synthesis and identification of chymotrypsin inhibitors. 环状肽模板组合文库:胰凝乳蛋白酶抑制剂的合成与鉴定。

Peptide research Pub Date : 1994-11-01

J Eichler, A W Lucka, R A Houghten