Peptide research最新文献

Modifications of the amide bond at position 3 in fMLP analogs select neutrophil functions. fMLP类似物中酰胺键3位的修饰选择中性粒细胞功能。

Peptide research Pub Date : 1996-11-01

S Spisani, A Breveglieri, E Fabbri, G Vertuani, O Rizzuti, G Cavicchioni

引用次数: 0

Adhesive peptides selected by phage display: characterization, applications and similarities with fibrinogen. 噬菌体展示选择的粘附肽:特性、应用及其与纤维蛋白原的相似性。

Peptide research Pub Date : 1996-11-01

K Gebhardt, V Lauvrak, E Babaie, V Eijsink, B H Lindqvist

{"title":"Adhesive peptides selected by phage display: characterization, applications and similarities with fibrinogen.","authors":"K Gebhardt, V Lauvrak, E Babaie, V Eijsink, B H Lindqvist","doi":"","DOIUrl":"","url":null,"abstract":"Phase clones with affinity for polystyrene/polyurethane magnetic particles were isolated from a 10-men peptide display library. Sequence analysis revealed that 40 out of 80 clones contained the consensus WXXWXXXW. Some of the selected phages showed high surface activity and adsorbed to plastic surfaces even in the presence of blocking agents or surfactants. Covalent attachment of a synthetic peptide (KG), carrying one of the selected sequences to alkaline phosphatase (AP) or bovine serum albumin (BSA) enhanced binding of AP to a wide range of materials and improved the ability of BSA to prevent binding of antibodies and phages to polystyrene. Interestingly, the WXXW/XXXW motif occurs in the beta- and gamma-chains of the natural \"adhesive\" protein fibrinogen, and a synthetic peptide carrying the gamma-chain 369-376 sequence turned out to have essentially the same binding properties as the KG peptide. Furthermore, adsorption in different types of polystyrene was similar for AP carrying either the KG or gamma-chain peptide intact fibrinogen and plasmin-generated fragment D1. The latter fragment contains two copies of the WXXWXXXW motif but lacks the alpha-chain: protuberances previously implicated in fibrinogen adsorption. Thus, our study may have revealed a hitherto unknown structural determinant for fibrinogen's adsorptivity, located in the 13-kDa C terminal region of the gamma-chain.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"269-78"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20006486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-assembly of cyclic peptides on a dendrimer: multiple cyclic antigen peptides. 树突上环状肽的自组装:多个环状抗原肽。

Peptide research Pub Date : 1996-11-01

J C Spetzler, J P Tam

{"title":"Self-assembly of cyclic peptides on a dendrimer: multiple cyclic antigen peptides.","authors":"J C Spetzler, J P Tam","doi":"","DOIUrl":"","url":null,"abstract":"Multiple cyclic antigen peptides (McAPs) are dendrimers that have branched, multiple closed-chain architectures. We describe an approach for their stepwise, solid-phase synthesis that permits a self-assembly of cyclization reactions of a McAP with four copies of cyclic peptides in solution after their cleavage from the resin with all protecting groups removed. The conceptual framework of our approach is the development of a method favoring intrachain cyclization based on ring-chain tautomerism between an N-terminal Cys and an aldehyde attached to the side chain of Lys to form a loop linked by a thiazolidine ring. The McAP precursor contains an amino Cys(St-Bu) and an internal Lys(Ser). A trialklyphosphine is used to deblock Cys(St-Bu) on the amino terminus and to effect the concomitant thiazolidine formation with the glyoxyl moiety obtained from an oxidative conversion of the Ser on the Lys side chain. Two McAPs, each containing cyclic peptides of 17 and 24 amino acid residues, have been prepared. To evaluate intrachain cyclization yields, a cleavage site as Asp-Pro incorporated at the COOH terminus of each monomeric loop and subsequently released after completion of the cyclization by treatment with formic acid at an elevated temperature. Reversed-phase high-performance liquid chromatography analyses of the liberated cyclic peptide monomer with synthetic standards support the theory that intrachain cyclization is the predominant cyclization pathway and validate the usefulness of this ring-chain tautomerization concept in the self-assembly of cyclic peptides on a branched peptide dendrimer.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"290-6"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20004045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-pore polydimethylacrylamide resin for solid-phase peptide synthesis: applications in Fmoc chemistry. 固相肽合成用大孔聚二甲基丙烯酰胺树脂:在Fmoc化学中的应用

Peptide research Pub Date : 1996-11-01

J T Sparrow, N G Knieb-Cordonier, N U Obeyseskere, J S McMurray

引用次数: 0

Effective use of free thiols as scavengers for HF cocktails to deprotect bromo- and chloroacetylated synthetic peptides. 有效地利用游离硫醇作为HF鸡尾酒清除剂去保护溴化和氯乙酰化合成肽。

Peptide research Pub Date : 1996-11-01

B B Ivanov, F A Robey

引用次数: 0

Solid-phase synthesis and characterization of human salivary statherin: a tyrosine-rich phosphoprotein inhibitor of calcium phosphate precipitation. 固相合成和表征人唾液施他汀蛋白:一种富含酪氨酸的磷酸钙沉淀磷蛋白抑制剂。

Peptide research Pub Date : 1996-11-01

T L Gururaja, M J Levine

{"title":"Solid-phase synthesis and characterization of human salivary statherin: a tyrosine-rich phosphoprotein inhibitor of calcium phosphate precipitation.","authors":"T L Gururaja, M J Levine","doi":"","DOIUrl":"","url":null,"abstract":"Human statherin, at low molecular weight (M 5380 Da. 43 amino acid residues) acidic tyrosine-rich phosphoprotein secreted mainly by salivary glands, has been synthesized successfully for the first time following standard solid-phase Fmoc chemistry. Synthesis of this phosphoprotein was accomplished using preformed phosphoserin building blocks. The phosphorylated protein thus synthesized was analyzed and compared with the native molecule and was found to have identical characteristics in its entirety, is evidenced by various analytical methods including mass spectral analysis. Analysis of both the synthetic and native statherin by circular dichroism spectroscopy showed an increase in helicity upon the addition of an organic cosolvent, trifluoroethanol (50%, vol/vol), indicating the presence of potentially amphipathic helical regions. Circular dichroism studies and hydrophobic moment calculations on this synthetic phosphoprotein revealed that the molecule adopts an amphipathic helical conformation at the N-terminus connected to a long poly-L-proline type II segment, which, in turn, is linked to an extended beta-strand. In correlation with previous studies. It appears that the strong binding affinity of statherin for hydroxyapatite can be attributed primarily to the N-terminal sequence, which prefers to adopted helical conformation and provides both electrostatic and hydrogen bonding interactions, thereby inhibiting its mineralization. Production of this highly homogenous synthetic statherin by chemical means may circumvent the prevailing obstacles encountered in conducting its tertiary structural investigations under various physiological conditions.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"283-9"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20006490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NMR study of five N-terminal peptide fragments of the vasoactive intestinal peptide: crucial role of aromatic residues. 血管活性肠肽的五个n端肽片段的核磁共振研究:芳香残基的关键作用。

Peptide research Pub Date : 1996-11-01

J F Goossens, P Cotelle, P Chavatte, J P Hénichart

引用次数: 0

Antifungal activity of synthetic 15-mer peptides based on the Rs-AFP2 (Raphanus sativus antifungal protein 2) sequence. 基于Raphanus sativus抗真菌蛋白2 (Rs-AFP2)序列合成的15聚肽的抗真菌活性

Peptide research Pub Date : 1996-11-01

G W De Samblanx, A Fernandez del Carmen, L Sijtsma, H H Plasman, W M Schaaper, G A Posthuma, F Fant, R H Meloen, W F Broekaert, A van Amerongen

{"title":"Antifungal activity of synthetic 15-mer peptides based on the Rs-AFP2 (Raphanus sativus antifungal protein 2) sequence.","authors":"G W De Samblanx, A Fernandez del Carmen, L Sijtsma, H H Plasman, W M Schaaper, G A Posthuma, F Fant, R H Meloen, W F Broekaert, A van Amerongen","doi":"","DOIUrl":"","url":null,"abstract":"Plant defensins are a class of cysteine-rich peptides of which several members have been shown to be potent inhibitors of fungal growth. A series of overlapping 15-mer peptides based on the amino acid sequence of the radish antifungal protein Rs-AFP2 have been synthesized. Peptides 6, 7, 8 and 9, comprising the region from cysteine 27 to cysteine 47 of Rs-AFP2 showed substantial antifungal activity against several fungal species (minimal inhibitory concentrations of 30-60 micrograms/mL), but no activity towards bacteria (except peptide 6 at 100 micrograms/mL). The active peptides were shown to be sensitive to the presence of cations in the medium and to the composition and pH of the medium. When present at a subinhibitory concentration (20 micrograms/mL), peptides 1, 7, 8 and 10 potentiated the activity of Rs-AFP2 from 2.3-fold to 2.8-fold. By mapping the characteristics of the active peptide on the structure of Rs-AFP2 as determined by nuclear magnetic resonance, the active region of the antifungal protein appears to involve beta-strands 2 and 3 in combination with the loop connecting those strands. A cyclized synthetic mimic of the loop, cysteine 36 to cysteine 45, was shown to have antifungal activity. Substitution of tyrosine 38 by alanine in the cyclic peptide substantially reduced the antifungal activity, indicating the importance of this residue for the activity of Rs-AFP2 as demonstrated carrier by mutational analysis.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"262-8"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20004044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystallographic characterization of tryptophan-containing peptide 3(10)-helices. 含色氨酸肽3(10)-螺旋的晶体学表征。

Peptide research Pub Date : 1996-11-01

C George, J L Flippen-Anderson, A Bianco, M Crisma, F Formaggio, C Toniolo

引用次数: 0

Assignment of the disulfide bonds in napin, a seed storage protein from Brassica napus, using matrix-assisted laser desorption ionization mass spectrometry. 利用基质辅助激光解吸电离质谱法鉴定甘蓝型油菜种子贮藏蛋白napin的二硫键。

Peptide research Pub Date : 1996-11-01

P M Gehrig, K Biemann

{"title":"Assignment of the disulfide bonds in napin, a seed storage protein from Brassica napus, using matrix-assisted laser desorption ionization mass spectrometry.","authors":"P M Gehrig, K Biemann","doi":"","DOIUrl":"","url":null,"abstract":"The sites of the disulfide bonds in a napin protein isolated from Brassica napus have been identified by proteolytic cleavage and subsequent peptide mapping by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Napins consist of two polypeptide chains containing two and six cysteine residues, respectively, that are held together by disulfide bonds. Upon initial cleavage of native napin by Endo-Lys-C, a disulfide-linked core complex of four peptides was obtained. This core peptide was isolated by reversed-phase HPLC and further digested by thermolysin, and the resulting fragments were identified by MALDI-MS. In a separate set of experiments, intact napin was subjected to proteolysis by thermolysin, and an isolated disulfide-linked peptide of interest was subdigested again using thermolysin. The combined data resulting from these experiments allowed the assignment of the disulfide linkages in a relatively abundant napin isoform, BngNAP1, apart from an ambiguity concerning the adjacent cysteines at positions 14' and 15' of the long chain. Two intermolecular disulfide bonds link Cys10 (short chain) with Cys25' (long chain) and Cys23 with Cys14' (or Cys15'), respectively. The long chain of napin contains two intramolecular disulfide bonds connecting Cys27' with Cys80' and Cys14' (or Cys15') with Cys72'.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"308-14"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20004498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0