Peptide research最新文献_第7页

High yield, directed immobilization of a peptide-ligand onto a beaded cellulose support. 高产、定向固定化肽配体到珠状纤维素载体上。

Peptide research Pub Date : 1994-11-01

D R Englebretsen, D R Harding

引用次数: 0

A general approach for identifying and cloning peptide synthetase genes. 鉴定和克隆肽合成酶基因的一般方法。

Peptide research Pub Date : 1994-09-01

K Turgay, M A Marahiel

引用次数: 0

Sequential order of T and B cell epitopes affects immunogenicity but not antibody recognition of the B cell epitope. T和B细胞表位的顺序影响免疫原性，但不影响B细胞表位的抗体识别。

Peptide research Pub Date : 1994-09-01

G Denton, F Hudecz, J Kajtár, A Murray, S J Tendler, M R Price

{"title":"Sequential order of T and B cell epitopes affects immunogenicity but not antibody recognition of the B cell epitope.","authors":"G Denton, F Hudecz, J Kajtár, A Murray, S J Tendler, M R Price","doi":"","DOIUrl":"","url":null,"abstract":"Synthetic peptide constructs, co-linearly linking a MUC1 mucin B cell epitope peptide to a known murine T cell epitope, both in T-B and B-T orientations, show that induction of high murine anti-MUC1 antibody titers is dependent on the presence and orientation of the T cell determinant. However, the sequential order of the epitopes does not affect binding of anti-B cell epitope antibodies to the constructs. Haplotype mismatching leads to a significant lowering of the anti-MUC1 antibody responses, implicating a central role for the T cell epitope in eliciting anti-B cell epitope responses. Secondary structure analysis by circular dichroism spectroscopy reveals the T-B construct to be partially ordered, while the B-T peptide adopts a highly ordered conformation in trifluoroethanol. These studies suggest that the sequential order of epitopes may significantly alter the immunogenicity of the peptide but may not necessarily affect its antigenicity. Immunogenicity of the peptide constructs may be governed by subtle differences in secondary structure, leading to variation in the way peptides are presented or processed within cells governing immune responses. These findings have relevance for the construction of peptides to be utilized as potential synthetic vaccines and for the design of peptide immunogens.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 5","pages":"258-64"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18540742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antisense-designed peptides: a comparative study focusing on possible complements to angiotensin II. 反义设计肽:一项聚焦于血管紧张素II可能补体的比较研究。

Peptide research Pub Date : 1994-07-01

D D Holsworth, J S Kiely, R S Root-Bernstein, R W Overhiser

引用次数: 0

Molecular dynamics conformations of deltorphin analogues advocate delta opioid binding site models. 三角洲海豚类似物的分子动力学构象提倡三角洲阿片结合位点模型。

Peptide research Pub Date : 1994-07-01

S D Bryant, M Attila, S Salvadori, R Guerrini, L H Lazarus

{"title":"Molecular dynamics conformations of deltorphin analogues advocate delta opioid binding site models.","authors":"S D Bryant, M Attila, S Salvadori, R Guerrini, L H Lazarus","doi":"","DOIUrl":"","url":null,"abstract":"Multi-site binding models for the delta opioid receptor were studied in vitro with [3H]DPDPE as the labeled ligand using analogues of deltorphin C (H-Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH2) altered at position 4. Modifications included a change in chirality (L- to D-Asp4), increased length of the anionic side-chain (Glu4), elimination of the charged group (Abu4), addition of an anionic group (Gla4), and change in backbone conformation (Pro4). All of the peptides had relatively high delta affinities (0.09 to 1.15 nM); the major variability in delta selectivity resided in changes in mu affinities (1.6 to 530 nM). Three analogues (Glu4, D-Asp4 and Pro4) revealed better fits to two-site binding models (Hill coefficients < 0.850 with narrow 95% confidence intervals and P < 0.0001). Deltorphin C and analogues containing Gla4 and Abu4 (which were weakly delta selective), as well as deltorphin A (H-Tyr-D-Met-Phe-His-Leu-Met-Asp-NH2), fitted one-site binding models. Molecular dynamics simulations performed on deltorphin C and Abu4 exhibited similarities in the tertiary structure of their low energy conformers, while differing from the three-dimensional structures of the analogues containing Glu4, D-Asp4 and Pro4 substitutions. The data provide support that the three-dimensional architecture of an opioid peptide is an important factor in the designation of delta opioid receptor subtypes.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 4","pages":"175-84"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18701217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of human osteocalcins: gamma-carboxyglutamic acid at position 17 is essential for a calcium-dependent conformational transition. 人骨钙素的合成:位置17的γ -羧谷氨酸是钙依赖性构象转变所必需的。

Peptide research Pub Date : 1994-07-01

M Nakao, Y Nishiuchi, M Nakata, T Kimura, S Sakakibara

引用次数: 0

Improvement of the applicability of carboxypeptidase Y in peptide synthesis by protein engineering. 用蛋白质工程技术提高羧基肽酶Y在多肽合成中的适用性。

Peptide research Pub Date : 1994-05-01

M Raaschou-Nielsen, U H Mortensen, K Olesen, K Breddam

{"title":"Improvement of the applicability of carboxypeptidase Y in peptide synthesis by protein engineering.","authors":"M Raaschou-Nielsen, U H Mortensen, K Olesen, K Breddam","doi":"","DOIUrl":"","url":null,"abstract":"Asn51 and Glu145 of (serine) carboxypeptidase Y function as binding sites for the C-terminal carboxylate group of peptide substrates, and Glu65 is involved in orienting these two amino acid residues. A series of mutants of carboxypeptidase Y where these three amino acid residues have been replaced were investigated for their applicability in transacylation reactions with amino acid esters as acceptors. With H-Val-OMethyl as the nucleophile, the fraction of aminolysis is significantly higher than with the corresponding amino acid, suggesting a beneficial effect of blocking the alpha-carboxylate group. Increasing the size of the alcohol moiety, i.e., -OEthyl, -OPropyl or OButyl, has an adverse effect on the binding of the nucleophile and on the maximum yield of aminolysis. Replacement of Asn51 and Glu145 with Ala or Gly has a pronounced beneficial effect both on binding and the maximum fraction of aminolysis. However, the results do not establish a specific type of interaction between the enzyme and these valine esters. It is probable that the rotational freedom around the ester bond allows multiple binding modes, depending on both the leaving group and type of structural change within the binding site. From a synthetic point of view, some of the mutant enzymes are much better than the wildtype enzyme when amino acid esters are used as nucleophiles.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 3","pages":"132-5"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19074588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of electrospray mass spectrometry, counterion distribution monitoring and N-(2-hydroxy-4-methoxybenzyl) backbone protection in peptide synthesis. 电喷雾质谱、反离子分布监测和N-(2-羟基-4-甲氧基苄基)主链保护在多肽合成中的作用。

Peptide research Pub Date : 1994-05-01

L C Packman, M Quibell, T Johnson

引用次数: 0

Covalent immunochemical membrane labeling of viable cells with K698-T708, a simian virus 40 tumor antigen-derived peptide. 猴病毒40肿瘤抗原衍生肽K698-T708对活细胞的共价免疫化学膜标记。

Peptide research Pub Date : 1994-05-01

R Hess, P Rau, M Schwab, S Paetzold, M Kuther, M Obert, H Agostini, C Haessler, D G Braun, G Brandner

{"title":"Covalent immunochemical membrane labeling of viable cells with K698-T708, a simian virus 40 tumor antigen-derived peptide.","authors":"R Hess, P Rau, M Schwab, S Paetzold, M Kuther, M Obert, H Agostini, C Haessler, D G Braun, G Brandner","doi":"","DOIUrl":"","url":null,"abstract":"The process of covalent immunochemical linking of viable cell membranes with a Simian Virus 40 (SV40) tumor antigen-derived undecapeptide, K(698)PPTPPPEPET(708) (KT), is described. The principle applied was the reaction of the lysine residue, K 698, of the undecapeptide with the succinimidyl moiety of a heterobifunctional linker molecule, N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) or sulfosuccinimidyl(4-iodo-acetyl)aminobenzoate (sulfo-SIAB). Thereby, upon release of N-hydroxy-succinimide, the rest of the linker molecule reacts covalently with the epsilon-NH2 group of lysine. Upon release of pyridyl-2-thion or hydrogen iodide, respectively, the second reactive moiety of the linker is then ready to form a covalent bond with SH-groups of cell membrane compounds. As a result, KT is covalently linked onto the cell membrane by an -SS- or an -S-bond, respectively. Binding is prevented by treatment of the candidate cells with iodoacetamide, an SH-reactive compound. This artificial cell membrane epitope can be demonstrated by surface immunofluorescence and by binding to immunomagnetic beads loaded with PAb1605, a KT-specific monoclonal antibody. Quantitation by cytofluorimetry shows some 10(4) KT molecules bound per cell, a number that is in the range of the number of SV40 tumor antigen molecules of genuine SV40-transformed mammalian cells.","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"7 3","pages":"146-52"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18530400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Side-chain deprotection of peptides synthesized onto a beaded cellulose support. 在纤维素珠状载体上合成的肽的侧链脱保护。

Peptide research Pub Date : 1994-05-01

D R Englebretsen, D R Harding

引用次数: 0