Parasitology International最新文献

{"title":"A novel chemokine binding protein 1-like gene is vital for the blood pool development and engorgement of the hard tick Haemaphysalis longicornis","authors":"Sana Sasaki ,&nbsp;Yuki Koike ,&nbsp;Kei Jimbo ,&nbsp;Takahiro Inoue ,&nbsp;Keiko Mizutani ,&nbsp;Kofi Dadzie Kwofie ,&nbsp;Hayato Kawada ,&nbsp;Fusako Mikami ,&nbsp;Hirotomo Kato ,&nbsp;Makoto Matsubayashi ,&nbsp;Md. Abdul Alim ,&nbsp;Anisuzzaman ,&nbsp;Naotoshi Tsuji ,&nbsp;Takeshi Hatta","doi":"10.1016/j.parint.2024.102990","DOIUrl":"10.1016/j.parint.2024.102990","url":null,"abstract":"<div><div>Tick saliva modulates host responses during a blood feeding process. We identified a novel chemokine binding protein 1-like (<em>HLCBP1-like</em>) gene from the salivary glands of the Asian longhorned tick, <em>Haemaphysalis longicornis</em>. The HLCBP1-like protein, lacking a well-defined conserved domain, showed structural similarity to evasin, a chemokine binding protein from the brown dog tick, <em>Rhipicephalus sanguineus</em>. A preliminary knockdown study of HLCBP1-like revealed that ticks with reduced expression of this gene, halted feeding in the early feeding phase, and did not fully-engorge, unlike the control dsRNA (malE) injected ticks. Also, knockdown ticks induced cellular immune responses in the host skin, similar to control dsmalE-injected ticks, but did not show hemorrhage. These findings suggest that HLCBP1-like may play a modulatory role in the slow feeding phase.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102990"},"PeriodicalIF":1.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invisible burden: Prevalence of Chagas disease in Latin American migrants in Turin, North-Western Italy – A STROBE compliant-cross-sectional study","authors":"Elisa Burdino ,&nbsp;Andrea Bernardi ,&nbsp;Francesco Cerutti ,&nbsp;Alessia Di Vincenzo ,&nbsp;Silvia Faraoni ,&nbsp;Gabriella Gregori ,&nbsp;Tiziano Allice ,&nbsp;Maria Grazia Milia ,&nbsp;Cristina Bobba ,&nbsp;Margherita Busso ,&nbsp;Guido Calleri ,&nbsp;Valeria Ghisetti","doi":"10.1016/j.parint.2024.102988","DOIUrl":"10.1016/j.parint.2024.102988","url":null,"abstract":"<div><h3>Objectives</h3><div>Chagas disease (CD), or American trypanosomiasis, is a parasitic disease caused by <em>Trypanosoma cruzi</em>, primarily transmitted by triatomine bugs. Increased travels and migrations introduced CD to non-endemic regions, including Europe. In Italy, the disease has raised public attention mainly in northern regions, where Latin American migrant population is larger.</div><div>This study aims to describe a CD screening initiative in Turin, Piedmont, during the World Chagas disease Day. We focused on migrants from CD endemic countries of Latin America.</div></div><div><h3>Methods</h3><div>A total of 63 serum samples from Latin American migrants were collected and tested for CD with a chemiluminescence immunoassay (CLIA) for initial screening, followed by Western Blot (WB) for confirmation, analyzing demographic data.</div></div><div><h3>Results</h3><div>Out of the 63 individuals screened, two tested positive for CD. Both positive cases were from Bolivia. Seropositive individuals were 54 and 72 years-old, and lived in Bolivia for 31 and 69 years.</div></div><div><h3>Conclusions</h3><div>The screening initiative suggests an underestimated prevalence of CD among Latin American migrants in Turin, underscoring the need of targeted public health interventions and emphasizing the importance of CD screening and awareness programs.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102988"},"PeriodicalIF":1.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and associated risk factors for Fasciola hepatica in sheep in Nile Delta of Egypt","authors":"Abdelfattah Selim ,&nbsp;Mohamed Marzok ,&nbsp;Hattan S. Gattan ,&nbsp;Mohammed H. Alruhaili ,&nbsp;Omar A. AlJabr ,&nbsp;Mohamed Salem","doi":"10.1016/j.parint.2024.102989","DOIUrl":"10.1016/j.parint.2024.102989","url":null,"abstract":"<div><div>Fascioliasis is a globally distributed zoonotic parasitic disease that affects ruminants, including sheep. This study conducted from January to December 2023, aimed to determine the prevalence of <em>Fasciola hepatica</em> in sheep across three governorates in Egypt's Nile Delta and to assess associated risk factors. A total of 455 serum samples were analyzed using a commercial ELISA kit, revealing antibodies against <em>F. hepatica</em> in 22.2 % of the tested sheep. There was no significant association between locality or sex and the seroprevalence of <em>F. hepatica</em> in sheep; however, the highest prevalence was observed in Kafr ElSheikh and in female sheep.</div><div>Concerning risk factors, poor conditioned sheep aged between 1 and 2 years showed 2.1 and 3.8 times higher of infection probability than others. In addition, the risk of <em>F. hepatica</em> infection in sheep increased significantly in winter season (OR = 6.6, 95 %CI: 2.6–16.8), in absence of prophylactic treatment (OR = 2.2, 95 %CI: 1.3–3.3) and in presence of snail (OR = 3, 95 %CI: 3–5.4). The existence of antibodies against <em>F. hepatica</em> in examined sheep raising in Nile Delta indicating that the disease is reported in the studied areas and needs to be managed on farms through control and preventative measures.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102989"},"PeriodicalIF":1.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shape variability and morphological discrimination of haptoral attachment structures in Urocleidoides spp. Mizelle et Price, 1964 (Monogenea: Dactylogyridae) parasitizing fish from neotropical rivers in southern Mexico","authors":"Abril Rodríguez-González ,&nbsp;Edgar F. Mendoza-Franco ,&nbsp;Juan Manuel Caspeta-Mandujano ,&nbsp;Guillermo Salgado-Maldonado","doi":"10.1016/j.parint.2024.102984","DOIUrl":"10.1016/j.parint.2024.102984","url":null,"abstract":"<div><div>The morphology of attachment organs in Monogenea is an essential source of evidence for taxonomic assessments and understanding of ecology and evolutionary history. It has been demonstrated that geometric morphometry analysis of the haptoral attachment organs provides rich and precise information about monogeneans morphology. In this study, we consider three monogenean species of <em>Urocleidoides</em>, <em>U. simonae</em>, <em>U. vaginoclaustrum</em>, <em>U</em>. <em>vaginoclaustroides</em>, and the <em>incertae sedis</em> species <em>U. strombicirrus</em> that parasitize fish of the Poeciliidae, Profundulidae and Characidae in neotropical rivers in southern Mexico. We aimed to identify new morphological characteristics that can be used to describe these populations with greater certainty and to test whether these characteristics present geographic variation. Anchor shape data (<em>n</em> = 69) were generated using geometric morphometric methods. We discriminated species using principal components and canonical variate analysis of shape data. Our results showed that there was discernible interspecific variation. The four species contributed four new morphological characteristics: anchor point length, short versus long anchor roots, elongated versus wide anchor shape, and the relative length of inner versus outer roots. Intraspecific variation was relatively low compared to interspecific anchor shape variation in <em>Urocleidoides</em> spp. These new morphological characteristics may also be useful for delimiting other similar species in Monogenea.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102984"},"PeriodicalIF":1.5,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiome–helminth–immune axis in autoimmune diseases","authors":"Chikako Shimokawa","doi":"10.1016/j.parint.2024.102985","DOIUrl":"10.1016/j.parint.2024.102985","url":null,"abstract":"<div><div>The global prevalence of autoimmune diseases has surged in recent decades. Consequently, environmental triggers have emerged as crucial contributors to autoimmune diseases, equally relevant to classical risk factors, such as genetic polymorphisms, infections, and smoking. Sequencing-based approaches have demonstrated distinct gut microbiota compositions in individuals with autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, type 1 diabetes mellitus (T1D), and systemic lupus erythematosus, compared to healthy controls. Furthermore, fecal microbiota transplantation and microbial inoculation experiments have supported the hypothesis that alterations in the gut microbiota can influence autoimmune responses and disease outcomes. Herein, we propose that intestinal helminths may serve as a critical factor in inducing alterations in the gut microbiota. The concept of helminth-mediated suppression of autoimmune diseases in humans is supported by substantial evidence, aligning with the long-standing “hygiene hypothesis.” This review focused on T1D to explore the interactions between parasites, gut microbiota, and the immune system—a topic that remains a black box within this intricate triangular relationship.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102985"},"PeriodicalIF":1.5,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplemental description of Benedenia seriolae (Yamaguti, 1934) Meserve, 1938 (Monogenoidea: Capsalidae) and first record of the parasite infecting a wild greater amberjack, Seriola dumerili (Risso, 1810) Hureau and Tortonese, 1973 (Carangiformes: Carangidae) from the northern Gulf of Mexico","authors":"Micah B. Warren ,&nbsp;Stephen A. Bullard","doi":"10.1016/j.parint.2024.102986","DOIUrl":"10.1016/j.parint.2024.102986","url":null,"abstract":"<div><div>We herein provide a supplemental description of <em>Benedenia seriolae</em> (Yamaguti, 1934) Meserve, 1938 (Monogenoidea: Capsalidae Baird, 1853) based on specimens we collected from the skin of wild-caught greater amberjack (<em>Seriola dumerili</em>) in the northern Gulf of Mexico (GOM). Our specimens of <em>B</em>. <em>seriolae</em> from the GOM resemble those of <em>Benedenia humboldti</em> Baeza, Sepúlveda, and González, 2019 (from yellowtail amberjack, <em>Seriola lalandi</em> Valenciennes, 1833 [Carangidae] off Chile) by having a large body (&gt;7000 μm in total length), non-overlapping anterior and posterior hamuli, an entirely sinistral and convoluted vas deferens, and a common genital pore as well as by lacking glands of Goto. It differs from <em>B</em>. <em>humboldti</em> by the shape of the penis tip. The <em>28S</em> rRNA gene sequence differed from that of <em>B. seriolae</em> (from Japan) by 8 bp (1.1 %) and <em>B. humboldti</em> by 13 bp (2.8 %–1.7 %). The phylogenetic analysis recovered our sequences of <em>B</em>. <em>seriolae</em> sister to conspecific sequences from Japan, with that clade sister to <em>B</em>. <em>humboldti</em> from Chile. <em>Benedenia</em> was paraphyletic, suggesting some systematic revision to the genus could be warranted. Of 25 <em>Benedenia</em> spp., only <em>Benedenia hendorffii</em> (von Linstow, 1889) Odhner, 1905 had been reported previously from the GOM. <em>Benedenia seriolae</em> had previously not been reported from wild greater amberjack (previous records from greater amberjack were from sea cages only). This is also the first record of <em>B</em>. <em>seriolae</em> from the Northwestern Atlantic Ocean (GOM).</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102986"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case of pulmonary cryptosporidiosis associated with pulmonary tuberculosis in a patient with advanced HIV infection","authors":"Adriana Topan ,&nbsp;Angela Monica Ionică ,&nbsp;Astrid Binder ,&nbsp;Violeta Briciu ,&nbsp;Mihaela Lupşe","doi":"10.1016/j.parint.2024.102983","DOIUrl":"10.1016/j.parint.2024.102983","url":null,"abstract":"<div><div><em>Cryptosporidium</em> spp., an important food and waterborne protozoan pathogen, can cause severe diarrhea and extraintestinal manifestations in immunocompromised individuals. The case of a 75 -years old patient diagnosed as a late presenter with advanced HIV infection and multiple opportunistic illnesses associated to HIV: cryptosporidiosis, intestinal and pulmonary, Cytomegalovirus colitis and pulmonary tuberculosis is presented. <em>Cryptosporidium parvum</em> was identified in fecal sample and bronchoalveolar lavage using rapid tests, PCR diagnosis, and sequencing, the two sequences being identical. In spite of adequate treatment for all associated infections, including Nitazoxanide for <em>Cryptosporidium</em> spp. infection, antiretroviral therapy, and management in the intensive care unit, the patient evolution was unfavorable, without immune reconstitution, leading to death on the 30th day of hospitalization. This case describes a rare localization of pulmonary cryptosporidiosis in association with pulmonary tuberculosis in a patient with advanced HIV infection. Clinicians should be aware of the possible pulmonary localization of this opportunistic parasite in immunodeficient patients.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102983"},"PeriodicalIF":1.5,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of immune-related genes in Pacific white snook (Centropomus viridis) gills infected with the monogenean parasite Rhabdosynochus viridisi","authors":"Marian Mirabent-Casals ,&nbsp;Víctor Hugo Caña-Bozada ,&nbsp;Francisco Neptalí Morales-Serna ,&nbsp;Juan Manuel Martínez-Brown ,&nbsp;Rosa María Medina-Guerrero ,&nbsp;Rubí Hernández-Cornejo ,&nbsp;Alejandra García-Gasca","doi":"10.1016/j.parint.2024.102981","DOIUrl":"10.1016/j.parint.2024.102981","url":null,"abstract":"<div><div>The parasite <em>Rhabdosynochus viridisi</em> (Platyhelminthes: Monogenea) infects the Pacific white snook <em>Centropomus viridis</em> gills and can cause adverse effects in the aquaculture industry. The immune responses of Pacific white snook to monogenean infections are poorly understood. Thus, this study aimed to identify differentially expressed genes (DEGs) in the gills of Pacific white snook juveniles experimentally infected with <em>R. viridisi</em>, emphasizing immune-related genes and pathways activated or suppressed during the infection. RNA sequencing was performed on the gills of uninfected (control) and infected fish. The algorithm Seq2Fun was selected without a reference transcriptome to map the reads to transcripts of fishes available from a database for gene orthologs (EcoOmics) and obtain the counting table. The ExpressAnalyst software was used for differential expression and functional analyses. A total of 20,106 transcripts were found, and 1430 (7 %) were differentially expressed genes (DEGs) between infected and control groups. We identified 860 (60 %) downregulated and 570 (40 %) upregulated genes. Thirteen canonical pathways after the Kyoto Encyclopedia of Genes and Genomes (KEGG) database were overrepresented, and most of the DEGs were downregulated, suggesting the inactivation of these pathways. The functions of most of the DEGs with higher fold change found in this study are poorly understood in fish. Even though the well-known pro-inflammatory cytokines remained unchanged in infected gills of <em>C. viridis</em>, and transforming growth factor β (<em>tgfβ</em>) was downregulated, interleukin-17 ligands <em>il17d</em> and <em>il17a/f1</em>, as well as C-X-C motif chemokine receptor 2 (<em>cxcr2</em>) genes were upregulated, indicating that the infection with <em>R. viridisi</em> promotes Th17-like immunity<em>.</em> Overexpression of plasma B cell activity markers such as immunoglobulin light chain-like genes and the v-set pre-B cell surrogate light chain 3 (<em>vpreb3</em>) was also detected in this study. The possible implications of DEGs related to calcium imbalance, hypoxia adaptation, hemostasis, and immunity are discussed. These results will support future studies to improve the prevention and treatment of monogenean infections in finfish aquaculture.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102981"},"PeriodicalIF":1.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and abundance of fleas and rodents of plague importance in the erstwhile endemic states and international seaports of India","authors":"Appadurai Daniel Reegan ,&nbsp;Anok Melvin Marshall ,&nbsp;K.P. Srilatha ,&nbsp;M. Malini ,&nbsp;Shivakumar S. Titti ,&nbsp;Rajesh L. Kademani ,&nbsp;Shubhangi Kulsange ,&nbsp;Monil Singhai","doi":"10.1016/j.parint.2024.102982","DOIUrl":"10.1016/j.parint.2024.102982","url":null,"abstract":"<div><div>Rodents are the reservoirs of the plague bacterium <em>Yersinia pestis</em>, which is transmitted through the bites of infected fleas. In this paper, we studied the distribution and abundance of fleas and rodents in the erstwhile endemic states and seaports of India from 2009 to 2019. The analysis showed that the <em>Rattus rattus</em> (<em>Rr</em>) was the major rodent species found in both Western (66.52 %) and Eastern (48.95 %) seaports of India and the other rodent species recorded were <em>Bandicota indica</em> (<em>Bi</em>) (16.68 % &amp; 34.24 %), <em>Bandicota bengalensis</em> (<em>Bb</em>) (8.88 % &amp; 16.02 %) <em>Rattus norvegicus</em> (<em>Rn</em>) (6.83 % &amp; 0.70 %), <em>Murines hurriane</em> (<em>Mh</em>) (1.08 % &amp; 0 %), <em>Mus musculus</em> (<em>Mm</em>) (0 % &amp; 0.09 %), respectively. Only two flea species namely, <em>Xenopsylla cheopis</em> (<em>X.c</em>) and <em>Xenopsylla astia</em> (<em>X.a</em>) were recorded during the study period in Eastern and Western seaports. Among them, <em>X. astia</em> was found to be the dominant species (55.56 %) and followed by <em>X. cheopis</em> (44.44 %). Further, the female population of <em>X. cheopis</em> and <em>X. astia</em> was higher in both Western and Eastern seaports than the male population. Similarly, <em>Rattus rattus</em> (<em>Rr</em>) (82.43 %,) was the major rodent species found in erstwhile endemic states of India, followed by <em>Tetera indica</em> (<em>Ti</em>) (11.99 %,). Only northern endemic states (HP and UK) reported <em>Rattus rattus terkiminasis</em> (<em>R.rt</em>) (0.01 %). Four flea species namely, <em>Xenopsylla cheopis</em> (64.89 %)<em>, Xenopsylla astia</em> (34.89 %)<em>, Stivalius ahalae</em> (0.18 %), and <em>Nosopsyllus fasciatus</em> (0.02 %) were recorded from endemic states. The presence of rodents and vector fleas warrants sustained plague surveillance in and around seaports and endemic states.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102982"},"PeriodicalIF":1.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of three important tick-borne pathogens in ticks and humans in Shaanxi Province, Northwest China","authors":"Rui Guan ,&nbsp;Na Wei ,&nbsp;Meng Cao ,&nbsp;Jian-Gong Yang ,&nbsp;Ru Wu ,&nbsp;Hui Li","doi":"10.1016/j.parint.2024.102980","DOIUrl":"10.1016/j.parint.2024.102980","url":null,"abstract":"<div><div>The investigation of important prevalent tick-borne pathogens is central to the control of tick-borne diseases. To understand the prevalence of <em>Borrelia burgdorferi</em> s.l., <em>Borrelia miyamotoi</em>, and the Alongshan virus in ticks and patients in Shaanxi Province, China, a total of 670 adult ticks and 524 patient blood samples were collected in the region between 2022 and 2023. In this study, PCR was used to estimate the prevalence of the three pathogens. The results revealed that the overall positive rates of <em>B. burgdorferi</em> s.l, <em>B. miyamotoi</em>, and ALSV infection in the collected ticks were 13.58 %, 2.46 %, and 4.17 %, respectively. Three genospecies of <em>B. burgdorferi</em> s.l. were detected in this study, namely, <em>B. garinii</em>, <em>B. afzelii</em>, and <em>B. bavariensis</em>, with positive rates of 4.63 %, 3.58 %, and 5.37 %, respectively. The prevalence rates of <em>B. miyamotoi</em>, ALSV  and <em>B. burgdorferi</em> s.l. in the patients were 0.38 %, 0.95 %, and 15 %, respectively. Moreover, <em>B. burgdorferi</em> s.l. and ALSV coinfection was also detected in three patients. Evolutionary analysis revealed that the isolate of <em>B. miyamotoi</em> belongs to the Asian type (Siberian type) and that of ALSV is closely related to that of Finland. To our knowledge, this is the first report on <em>B. miyamotoi</em> and ALSV detection in both ticks and humans in Shaanxi Province; the prevalence indicates that the regions in this province might be endemic areas. The results also suggest that humans in these regions are at risk of infection with these three tick-borne diseases.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102980"},"PeriodicalIF":1.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}