Parasitology International最新文献

{"title":"Microscopy and molecular survey of Hepatozoon spp. in domestic cats and their ticks: First report of H. silvestris from Türkiye","authors":"Zuhal Önder ,&nbsp;Didem Pekmezci ,&nbsp;Alparslan Yıldırım ,&nbsp;Gökmen Zafer Pekmezci ,&nbsp;Önder Düzlü ,&nbsp;Zeynep Nurselin Kot ,&nbsp;Gamze Nur Konacoğlu ,&nbsp;Gamze Yetişmiş ,&nbsp;Abdullah İnci","doi":"10.1016/j.parint.2024.102979","DOIUrl":"10.1016/j.parint.2024.102979","url":null,"abstract":"<div><div>Hepatozoonosis is a tick-borne infection caused by protozoan species of the <em>Hepatozoon</em> genus in the family Hepatozoidae. This study aimed to determine the presence and prevalence of <em>Hepatozoon</em> spp. in domestic cats and their ticks, to characterize the genotypes, to reveal phylogenetic relationships, and to assess the risk factors associated with infection. Blood samples were collected in EDTA tubes from 311 cats that were brought to the Veterinary Faculty, Animal Hospital for treatment or routine control purposes. Smears were prepared from the buffy coat and <em>examined</em> microscopically for the presence of <em>Hepatozoon</em> spp. <em>gamonts.</em> Ticks were collected during the clinical examination of the cats and the species were identified. Genomic DNA obtained from buffy coats and ticks were analyzed using PCR analyses targeting the 18S rRNA gene region of <em>Hepatozoon</em> spp. The results showed that <em>Hepatozoon</em> spp. gamonts in neutrophils were determined in five samples as 1.6 % (5/311). A total of 58 (18.64 %, 58/311) were positive for the PCR analyses. Twelve ticks were collected from the cats, and all were identified as <em>Rhipicephalus turanicus. Hepatozoon</em> spp. were found in seven (58.3 %) ticks by molecular analyses. Sequence analysis of the 18S rRNA gene region of 58 positive isolates revealed the presence of <em>H. felis</em> (<em>n</em> = 25, 43.1 %) and <em>H. silvestris</em> (<em>n</em> = 33, 56.9 %) species in cats and <em>H. felis</em> in <em>Rh. turanicus</em> (<em>n</em> = 7). This study also presents the first report of <em>H. silvestris</em> in domestic cats from Türkiye. Phylogenetic analyses showed that our <em>H. felis</em> isolates clustered in <em>H. felis</em> genotype I and our <em>H. silvestris</em> were stated in the same cluster with Italian isolates.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102979"},"PeriodicalIF":1.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal detection of pathogens in honeybees kept in natural and laboratory conditions","authors":"Magdalena Kunat-Budzyńska ,&nbsp;Emilia Łabuć ,&nbsp;Aneta A. Ptaszyńska","doi":"10.1016/j.parint.2024.102978","DOIUrl":"10.1016/j.parint.2024.102978","url":null,"abstract":"<div><div>The honeybee is one of the most important pollinators in the world. The frequently observed poor health of honeybee colonies can be caused by various factors, e.g. environmental pollution, nutritional stress, and climate changes. Moreover, honeybees are constantly exposed to a wide spectrum of pathogens, such as parasites, bacteria, and viruses. We examined the occurrence of various diseases in different-aged worker honeybees from two colonies kept in natural and laboratory conditions during spring, summer, and autumn in Poland. The honeybees were examined by PCR to detect infection with selected pathogens: <em>Nosema ceranae, N. apis, N. bombi, Acarapis woodi,</em> trypanosomatids, and neogregarines (<em>Mattesia</em> or <em>Apicystis</em> species) and by RT-PCR to identify deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV). DWV and <em>N. ceranae</em> turned out to be the dominant pathogens. Trypanosomatids and BQCV were also found in several samples. We did not detect the presence of the other pathogens: <em>N. apis, N. bombi, A. woodi,</em> neogregarines, or ABPV. As shown in the present study, the dynamics and occurrence of pathogens are influenced by keeping conditions, honeybee age, and seasonality.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102978"},"PeriodicalIF":1.5,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Miniature pigs as the intermediate host for Taenia asiatica","authors":"Hiroshi Yamasaki ,&nbsp;Hiromu Sugiyama ,&nbsp;Yasuyuki Morishima ,&nbsp;Munehiro Okamoto","doi":"10.1016/j.parint.2024.102977","DOIUrl":"10.1016/j.parint.2024.102977","url":null,"abstract":"<div><div><em>Taenia asiatica</em>, utilizing pigs as an intermediate host, degenerates and/or calcifies within a few months after infection in pigs, whereas <em>Taenia solium</em>, also using pigs as an intermediate host, can develop into a mature metacestode within a couple of months and can survive for prolong periods in pigs. This raises the question of whether pigs are suitable intermediate hosts for <em>T. asiatica.</em> The host-parasite relationships between <em>T. asiatica</em> and pig strains, such as infection rates and development of metacestodes, have been reported in previous studies: however, little is known about the pathological changes that occur in <em>T. asiatica</em> metacestodes in pigs. Therefore, in the present study, the pathological changes in <em>T. asiatica</em> within 30 days of infection were observed using CLAWN miniature pigs as model animals. Metacestodes were observed on the diaphragmatic surface and throughout the parenchyma of the pig liver 9 days after infection; however, these metacestodes were surrounded by eosinophilic abscesses, and some had already begun to degenerate. By day 20 and 30 post-infection, metacestodes were surrounded by eosinophilic abscesses and had completely degenerated without forming a scolex. These results indicate that although <em>T. asiatica</em> infected miniature pigs, the metacestodes degenerated owing to strong immune responses from the pigs. Therefore, the CLAWN miniature pig are not a suitable intermediate host for <em>T. asiatica.</em> The possible reasons why <em>T. asiatica</em> metacestodes were degenerated and the potential roles of pigs in transmitting the parasite to humans in <em>T. asiatica</em>-endemic regions are discussed in this study. Additionally, data debating whether pigs are suitable intermediate hosts for <em>T. asiatica</em> are provided.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102977"},"PeriodicalIF":1.5,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elimination of schistosomiasis mekongi in reach for Lao PDR: The last patient with severe disease?","authors":"Phonesavanh Southisavath ,&nbsp;Kerstin Kling ,&nbsp;Anousin Homsana ,&nbsp;Nicole Probst-Hensch ,&nbsp;Daniel Henry Paris ,&nbsp;Somphou Sayasone ,&nbsp;Peter Odermatt","doi":"10.1016/j.parint.2024.102976","DOIUrl":"10.1016/j.parint.2024.102976","url":null,"abstract":"<div><div>Schistosomiasis mekongi is endemic in a restricted area in Northern Cambodia and the Southern Lao People's Democratic Republic. Severe hepatobiliary morbidity is associated with chronic untreated <em>S. mekongi</em> infection. Since the 1980s extensive control efforts have been employed in endemic areas, resulting in substantial reduction of infection rates and disease burden. We report on a patient with a fatal course of clinically-assessed chronic schistosomiasis. This report underscores that patients with severe chronic Mekong schistosomiasis may still exist and may need treatment support.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102976"},"PeriodicalIF":1.5,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filling the void: Morphological and molecular phylogenetic analyses of helminths assemblage from the Egyptian egret Bubulcus ibis","authors":"Nada A. Ibrahim ,&nbsp;Ismail M. Shalaby ,&nbsp;Mahi A. Ghobashy ,&nbsp;Azza A. Taeleb ,&nbsp;Elham A. Elkhawass","doi":"10.1016/j.parint.2024.102972","DOIUrl":"10.1016/j.parint.2024.102972","url":null,"abstract":"<div><div>The cattle egret (<em>B. ibis</em>) as a common wader birds in Egypt, they act a sole reservoir for many parasites and play a vital role in their life cycle and their distribution in their environment. The study was conducted from September 2020 to August 2021. A total of 180 <em>B. ibis</em> were collected from Al Qantara Gharb, Ismailia province, Egypt. Parasite species identification infecting cattle egret included morphological and morphometric characteristics based on light and scanning microscopy. Additionally, utilizing the partial small subunit ribosomal RNA (18S rRNA, ITS2 and ITS1) gene sequence, maximum parsimony was used to infer the phylogeny of the recovered species. The morphological and molecular studies revealed three helminths. Only one nematode (<em>Desportesius invaginatus</em>, linstow,1901) and two trematodes (<em>Patagifer bilobus</em>, Dietz,1909 and <em>Apharyngostrigea cornu</em>, Zeder,1800) have been identified. The cattle egret (<em>B. ibis</em>) are protagonists in the life cycle of many parasites. The study is considered the first in Egypt to fill the gap of phylogenetic analysis of three helminths; two of them (<em>A. cornu</em> and <em>P. bilobus</em>) were the first to be molecular phylogenetically analyzed in Egypt. The molecular data provided set the conspecific relation of the three identified helminths species with other related helminths. The new identified sequences will help in founding the basis for forthcoming identifications of other helminths species from cattle egret in Egypt and prospective view to possible parasitic assemblage affecting egret population and other animal populations in their environment.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102972"},"PeriodicalIF":1.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metazoan parasite fauna of grey mullet (Mugilidae) from the coastal waters of Vietnam","authors":"Evgenija Dmitrieva ,&nbsp;Violetta Yurakhno ,&nbsp;Vo Thi Ha ,&nbsp;Elina Chelebieva ,&nbsp;Ekaterina Vodiasova","doi":"10.1016/j.parint.2024.102975","DOIUrl":"10.1016/j.parint.2024.102975","url":null,"abstract":"<div><div>A total of 201 fishes of the family Mugilidae caught off central Vietnam (Nha Trang Bay) were examined for parasites. Eight species of Mugilidae were identified based on cytb and 16S rRNA sequences. Protozoa were not revealed. The metazoan parasites belong to six higher taxa. Myxozoa Grassé, 1970 is represented by six genera, three of which were first found in grey mullets off Vietnam. The specimens of Monogenea Van Beneden, 1858 belong to two genera: <em>Ligophorus</em> Euzet et Suriano, 1977 and <em>Metamicrocotyla</em> Yamaguti, 1953. Twelve species of the former were recorded for the first time both in the coastal waters of Vietnam and in the East Sea. Trematoda Rudolphi, 1808 is represented by the members of the four families, of which only three species, <em>Haplosplanchnus pachysoma</em> (Eysenhardt, 1892), <em>Pseudohaploporus planilizum</em> Atopkin, Besprozvannykh, Ha, Nguyen, Nguyen &amp; Chalenko, 2019 and <em>Spiritestis arabii</em> Nagaty, 1948, were identified. The latter was first recorded in the East Sea. Acanthocephala Rudolphi, 1808 specimens belong to the species <em>Neoechinorhynchus dimorphospinus</em> Amin &amp; Sey, 1996. Crustacea Brünnich, 1772 are represented by five families, of which only the copepod <em>Lernanthropsis mugilii</em> (Shishido, 1898) and the isopod <em>Nerocila orbignyi</em> (Guérin, 1832) were identified. Hirudinea Savigny, 1822 were not determined to genus and species level. A total of 25 species of parasites were found in the Mugilidae fishes examined in Nha Trang Bay. In general, 96 species of parasites are currently documented in these fishes in the East Sea, 55 of which occur off Vietnam. As a result of this study, carried out off central Vietnam, 16 parasite species were recorded in grey mullet for the first time in Vietnamese coastal waters and 15 for the first time in the East Sea. Thus, extending the geographical range of the studies may result in new records of parasites in these fishes.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102975"},"PeriodicalIF":1.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new species of Bivesiculoides (Digenea: Bivesiculidae) infecting atherinid fishes of the Great Barrier Reef, Queensland, Australia","authors":"Clarisse Louvard ,&nbsp;Scott C. Cutmore ,&nbsp;Thomas H. Cribb","doi":"10.1016/j.parint.2024.102974","DOIUrl":"10.1016/j.parint.2024.102974","url":null,"abstract":"<div><div>We describe a new species of Bivesiculidae, <em>Bivesiculoides maiae</em> n. sp., from <em>Hypoatherina tropicalis</em> (Whitley) (Atherinidae) collected from off Heron Island (southern Great Barrier Reef, Queensland, Australia). <em>Bivesiculoides maiae</em> n. sp. is morphologically consistent with <em>Bivesiculoides</em> Yamaguti, 1938 in the entirely pre-testicular position of its uterus, and the possession of caeca and vitelline fields that extend posteriorly to level with the anterior extremity of the testis. The new species is morphologically distinct from the six known <em>Bivesiculoides</em> species in body size and shape, and shape of the pharynx and testis. <em>Bivesiculoides maiae</em> n. sp. is genetically distinct from the only other sequenced <em>Bivesiculoides</em> species, <em>Bivesiculoides fusiformis</em> Cribb, Bray &amp; Barker, 1994, with which it occurs sympatrically at Heron Island. A review of related species allows two systematic recombinations. In view of the pre-testicular position of its uterus, we recombine <em>Bivesicula hepsetiae</em> Manter, 1947 as <em>Bivesiculoides hepsetiae</em> (Manter, 1947) n. comb. In view of its obtriangular body shape, round pharynx, strongly elongated testis, and the position of its ovary opposite the testis, we recombine <em>Bivesiculoides triangularis</em> Machida &amp; Kuramochi, 2000 as <em>Treptodemoides triangularis</em> (Machida &amp; Kuramochi, 2000) n. comb. Host-specificity of species of <em>Bivesiculoides</em> and their geographic distributions are discussed.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102974"},"PeriodicalIF":1.5,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trematode metacercariae parasitic in the estuarine crustacean Cyathura muromiensis Nunomura, 1974 (Peracarida: Isopoda: Anthuroidea)","authors":"Shoki Shiraki ,&nbsp;Keiichi Kakui","doi":"10.1016/j.parint.2024.102973","DOIUrl":"10.1016/j.parint.2024.102973","url":null,"abstract":"<div><div>This is the first report of trematodes parasitic in the estuarine isopod crustacean <em>Cyathura muromiensis</em>, and the fourth report from anthuroid isopods worldwide. From 52 of 54 host individuals collected qualitatively on the tidal flat of the Muromi River estuary, Fukuoka, Japan, 389 cysts of metacercariae were extracted (sample prevalence = 96.3 %). Host individuals contained from one to 71 metacercarial cysts. The range in cyst diameter was 172.3–252.1 μm, and the distribution of cyst sizes contained only one component. Cysts occurred in pereonites 2–7 and the pleon of the host, but not in the head, pereonite 1, or telson. There was no correlation between the number of cysts and host sex or size. Sequences of the nuclear “ITS1 region,” from the 3´ region of 18S rRNA to the 5´ region of internal transcribed spacer I gene (ITS1), from five cysts ranging in size from nearly the lowest diameter to the greatest diameter in our sample showed p-distances of 0.0–0.2 %, suggesting that all cysts obtained were conspecific. A phylogenetic reconstruction based on nuclear 28S rRNA gene sequences showed that the trematode belongs in the genus <em>Microphallus</em>. The definitive host of our trematode species is likely a bird, since the definitive hosts of microphallids are chiefly birds, and birds are known to prey on <em>Cyathura</em> isopods.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102973"},"PeriodicalIF":1.5,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of trypanosomes in tsetse fly caught in two “silent” sleeping sickness foci of Bafia and the Manoka Island in Cameroon","authors":"Sartrien Kanté Tagueu PhD ,&nbsp;Jean Arthur Mbida Mbida assistant professor ,&nbsp;Florentine Mamia Grace ,&nbsp;Rolin Mitterran Kamga Ndéfo PhD ,&nbsp;Gael Atangana Bita ,&nbsp;Rostand Joël Atiokeng Tatang PhD ,&nbsp;Alphonse Acho ,&nbsp;Flobert Njiokou Pr ,&nbsp;Gustave Simo Pr","doi":"10.1016/j.parint.2024.102970","DOIUrl":"10.1016/j.parint.2024.102970","url":null,"abstract":"<div><h3>Background</h3><p>Surveillance of “silent” human African Trypanosomiasis (HAT) foci is important for the achievement of the World Health Organization (WHO) goal of interrupting the transmission of this disease by 2030. It is in this context that this study was carried out to determine the trypanosome species circulating in the “silent” HAT foci of Bafia and the Manoka island in Cameroon.</p></div><div><h3>Methods</h3><p>In the Bafia and Manoka HAT foci, georeferenced pyramidal traps were used to trap tsetse flies. After DNA extraction from each whole fly, molecular tools were used to detect different trypanosome species as well as the origin of tsetse fly blood meals. Geographical information system was used to map the trypanosome infections and entomological data and to localize areas at high risk for trypanosome transmission.</p></div><div><h3>Results</h3><p>For this study, 1683 tsetse flies were caught and the relative apparent densities was 2.96: 0.03 in the Bafia HAT focus and 5.23 in the Manoka island. For the molecular identification of trypanosomes, 708 non-teneral tsetse flies (8 from Bafia and 700 from Manoka) were randomly selected. The overall trypanosome infection rate was 7.34 % with no infection in the Bafia HAT focus. Among the analysed flies, 4.57 % had trypanosomes of the subgenus <em>Trypanozoon</em> while 4.1 % and 1.13 % had respectively <em>T. congolense</em> and <em>T. vivax</em>. The most common mixed infections were the combination of trypanosomes of the subgenus <em>Trypanozoon</em> and <em>T. congolense</em>. Of the 708 tsetse flies analysed, 134 (18.93 %) tsetse flies were found with residual blood meals, 94 % and 6 % were respectively from humans and dogs. The trapping sites of Plateau, Sandje and Hospital appeared as the areas where contact with tsetse flies is most common.</p></div><div><h3>Conclusion</h3><p>This study revealed a discrepancy in the abundance tsetse flies as well as the trypanosome infection rates in tsetse of the two “silent” HAT foci of Cameroon. The detection of different trypanosome species in tsetse from the Manoka Island highlights their transmission. The high percentage of human blood meals in tsetse flies indicates an important contact between tsetse flies and human; emphasizing the risk of trypanosome transmission to human in this island.</p></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102970"},"PeriodicalIF":1.5,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caligus minimus Otto, 1821 (Copepoda: Caligidae): A commercially important but poorly described parasite of cultured European Sea Bass, Dicentrarchus labrax (Linnaeus, 1758)","authors":"Alper Yanar ,&nbsp;Seyit Ali Kamanli ,&nbsp;Serdar Sönmez ,&nbsp;İlhem Hamdi ,&nbsp;Argun Akif Özak ,&nbsp;Geoffrey Allan Boxshall","doi":"10.1016/j.parint.2024.102964","DOIUrl":"10.1016/j.parint.2024.102964","url":null,"abstract":"<div><div><em>Caligus minimus</em> Otto, 1821 has been known for over two centuries and it is the second oldest of the approximately 275 species of <em>Caligus</em> O. F. Müller, 1985. Despite the numerous records of this species from European waters, it has never been fully described to modern standards. The lack of a comprehensive modern description has resulted in numerous misidentifications, even in recently published reports, and this is especially problematic for a species that is known to have a significant economic impact in aquaculture. This study presents a detailed description of both sexes and documents newly observed features of <em>C. minimus</em> collected from the buccal cavity of farmed European Sea Bass (ESB), <em>Dicentrarchus labrax</em> (Linnaeus, 1758). The morphology of <em>C. minimus</em> was examined using light microscope (LM), scanning electron microscope (SEM), and confocal laser scanning microscope (CLSM), and new details are revealed regarding the structure and ornamentation of the marginal membrane of the cephalothorax, maxilliped, antenna, sternal furca, abdomen, and legs 1, 3, 4, and 6. The ornamentation of the marginal membrane of the cephalothorax is unique and its impact on the functioning of the cephalothoracic sucker requires further investigation. Additionally, partial <em>COI</em> gene region sequences were obtained from four individuals of <em>C. minimus</em> and provided for future references. A phylogenetic analysis was conducted in conjunction with <em>Caligus</em> sequences available in the NCBI GenBank database.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"104 ","pages":"Article 102964"},"PeriodicalIF":1.5,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}