Parasitology International最新文献

Molecular survey of zoonotic protozoa in free-ranging marmosets (Callithrix penicillata) from Central Brazil: implications for One Health surveillance. 巴西中部自由放养狨猴（青毛猴）人畜共患原虫的分子调查：对一个卫生监测的意义。

IF 2.1 4区医学

Parasitology International Pub Date : 2026-10-21 DOI: 10.1016/j.parint.2026.103411

Ludmilla Melanie, Vitória França Dos Santos Pessoa, Davi E R Sousa, Isabel L Macêdo, Liz A Cerqueira, Marcio B Castro, José Fabrício de Carvalho Leal, Nadjar Nitz, Mariana Hecht, Luciana Hagström

{"title":"Molecular survey of zoonotic protozoa in free-ranging marmosets (Callithrix penicillata) from Central Brazil: implications for One Health surveillance.","authors":"Ludmilla Melanie, Vitória França Dos Santos Pessoa, Davi E R Sousa, Isabel L Macêdo, Liz A Cerqueira, Marcio B Castro, José Fabrício de Carvalho Leal, Nadjar Nitz, Mariana Hecht, Luciana Hagström","doi":"10.1016/j.parint.2026.103411","DOIUrl":"https://doi.org/10.1016/j.parint.2026.103411","url":null,"abstract":"<p><p>Urban expansion and environmental fragmentation have intensified contact among wildlife, domestic animals, and humans, reshaping infectious disease dynamics at the human-wildlife interface. Non-human primates (NHP), particularly the black-tufted marmoset (Callithrix penicillata), readily adapt to anthropogenic landscapes and may serve as sentinel hosts for zoonotic pathogens. This study investigated the occurrence of Leishmania spp., Plasmodium spp., Toxoplasma gondii, and Trypanosoma cruzi. in 232 free-ranging C. penicillata found dead in urban and peri-urban areas of the Federal District, central Brazil, between 2018 and 2023. Liver samples were analyzed by quantitative PCR. Plasmodium spp. was not detected (0/232). In contrast, 39.7% (92/232) of marmosets were positive for T. gondii, 32.4% (69/213) for Leishmania spp., and 21.1% (47/223) for T. cruzi. Overall, 71.6% of the animals tested positive for at least one protozoan agent, and 21.2% had coinfections, most commonly Leishmania spp. plus T. gondii. Positive cases were widely distributed across administrative regions, with evidence of spatiotemporal clustering in some urbanized areas. Parasitic loads varied substantially, and most infected animals showed no lesions attributable to these infections. These findings demonstrate the widespread circulation of zoonotic protozoa in free-ranging marmosets inhabiting anthropized environments and reinforce their epidemiological relevance within a One Health framework. Surveillance of urban NHP populations can contribute to understanding transmission networks and environmental contamination, thereby supporting public health strategies for the prevention and control of zoonotic diseases in central Brazil.</p>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":" ","pages":"103411"},"PeriodicalIF":2.1,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of feline sparganosis caused by Spirometra asiana (Cestoda: Diphyllobothriidae) discovered during left forelimb amputation. 左前肢截肢时发现由亚洲螺虫引起的猫spargosis 1例。

IF 2.1 4区医学

Parasitology International Pub Date : 2026-10-19 DOI: 10.1016/j.parint.2026.103412

Mizuki Sasaki, Toko Kanetaka, Ko Hashizume, Satoshi Mitani, Hiroyuki Morioka, Moeko Sato

引用次数: 0

Effect of α-tocopheryloxyacetic acid, a mitocan, on mice infected with Trypanosoma congolense. α-生育酚氧乙酸对小鼠感染刚果锥虫的影响。

IF 2.1 4区医学

Parasitology International Pub Date : 2026-10-14 DOI: 10.1016/j.parint.2026.103409

Tatsuki Kuramoto, Kasumi Kawamura, Aiko Kume, Keisuke Suganuma, Shunji Kasai, Hiroshi Suzuki, Rika Umemiya-Shirafuji

{"title":"Effect of α-tocopheryloxyacetic acid, a mitocan, on mice infected with Trypanosoma congolense.","authors":"Tatsuki Kuramoto, Kasumi Kawamura, Aiko Kume, Keisuke Suganuma, Shunji Kasai, Hiroshi Suzuki, Rika Umemiya-Shirafuji","doi":"10.1016/j.parint.2026.103409","DOIUrl":"10.1016/j.parint.2026.103409","url":null,"abstract":"<p><p>The genus Trypanosoma is vulnerable to oxidative stress, and reactive oxygen species (ROS) suppress Trypanosoma proliferation. We investigated the effects of alpha-tocopheryloxyacetic acid (α-TEA), a stable vitamin E-derived mitocan, on the course of Trypanosoma congolense IL-3000 infection in mice and in an in vitro culture. C57BL/6 J male mice infected with 3 × 10<sup>4</sup> T. congolense IL-3000 cells were fed a 0.25% or 0.75% α-TEA diet for 7 or 28 days from day 3 of infection. The survival duration of all of the α-TEA-treated mice was significantly longer than that of the control mice fed a regular diet (p < 0.05). Similar results were obtained when α-TEA (14 mg/day) was orally administered to mice via a feeding needle 1×/day for 5 days from day 3 after infection; we then observed that α-TEA effectively inhibited the growth of T. congolense in a dose-dependent manner (p < 0.05). To assess the mechanisms of action of α-TEA, we conducted annexin V (AV)/propidium iodide (PI) labeling and a JC-1 labeling lactate assay, plus a 2',7'-dichlorofluorescein diacetate (DCFH-DA)/mtSOX Deep Red (mtSOX) labeling assay. Treatment with α-TEA induced the apoptosis of T. congolense, decreased the mitochondrial membrane potential (p < 0.05), and produced ROS in mitochondria (p < 0.05). Thus, α-TEA directly suppressed the proliferation of T. congolense in mice by stimulating a mitochondrion of T. congolense to produce ROS. These results indicate that α-TEA might be a potent candidate ingredient for an oral drug to treat the parasitic infection trypanosomiasis, with new mechanisms of action.</p>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":" ","pages":"103409"},"PeriodicalIF":2.1,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeny and systematics of South American freshwater parasitic isopods: evidence for elevating Artystoninae to family rank. 南美淡水寄生等足类动物的系统发育和系统分类学：青蒿科提升到科级的证据。

IF 2.1 4区医学

Parasitology International Pub Date : 2026-10-13 DOI: 10.1016/j.parint.2026.103407

Martin M Montes, Wilson S Serra Alanis, Jorge A Barneche, Fabrizio Scarabino, Guillermo Teran, Gaston Aguilera, Felipe Alonso, Cecilia Rodrígues-Haro, Dario Balcazar, Exequiel Oscar Furlan, German Reig Cardarella

{"title":"Phylogeny and systematics of South American freshwater parasitic isopods: evidence for elevating Artystoninae to family rank.","authors":"Martin M Montes, Wilson S Serra Alanis, Jorge A Barneche, Fabrizio Scarabino, Guillermo Teran, Gaston Aguilera, Felipe Alonso, Cecilia Rodrígues-Haro, Dario Balcazar, Exequiel Oscar Furlan, German Reig Cardarella","doi":"10.1016/j.parint.2026.103407","DOIUrl":"https://doi.org/10.1016/j.parint.2026.103407","url":null,"abstract":"<p><p>South America harbors the highest diversity of freshwater parasitic isopods within Cymothoidae; however, their phylogenetic relationships remain poorly understood due to limited genetic data. In this study, we generated mitochondrial cytochrome c oxidase subunit I (COI) sequences for the genera Artystone, Riggia, Braga, and Telotha to reconstruct the evolutionary relationships of South American freshwater cymothoids. Bayesian phylogenetic analyses recovered a distinct and well-supported freshwater clade, with Alitropus typus at its base, followed by Telotha, Asotana, Braga, Riggia, and Artystone. The molecular phylogenetic results presented here, combined with previously recognized differences in ontogenetic development and mouthpart morphology support the monophyly and distinctiveness of Artystoninae. Accordingly, we propose elevating this subfamily to family rank as Artystonidae. Specimens previously identified as Riggia puyensis exhibit pleotelson morphology and segmental articulation diagnostic of Artystone rather than Riggia, and cluster genetically with Artystone trysibia. Accordingly, Riggia puyensis is transferred to Artystone as Artystone puyensis n. comb. The recovered phylogeny suggests an evolutionary transition from external attachment to burrowing parasitism, with burrowing interpreted as a derived condition within this freshwater clade. Overall, our results support a single colonization of South American freshwater environments followed by subsequent diversification and provide an updated systematic framework for freshwater cymothoids, highlighting the value of integrative taxonomic approaches for resolving complex parasitic lineages.</p>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":" ","pages":"103407"},"PeriodicalIF":2.1,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and pathogen surveillance in chigger mites across Brazil's five biogeographic regions 巴西五个生物地理区域恙螨的多样性和病原体监测。

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-02-05 DOI: 10.1016/j.parint.2026.103248

Isabella Pereira Pesenato , Ricardo Bassini-Silva , Ana Cláudia Calchi , Sueli Akemi Taniwaki Miyagi , Ester Nascimento da Costa , Carlos Cezar Trigo Nasser-Felix , Glauber Meneses Barboza de Oliveira , Mauricio Claudio Horta , Gabriela Cousandier , Jonas Sponchiado , Ralph Maturano , Graziela Virginia Tolesano-Pascoli , Renata D. Alquezar , Camile Lugarini , Ariane Ferreira , Luciano Moreira-Lima , Erika Hingst-Zaher , Josivania Soares Pereira , Darci Moraes Barros-Battesti , Marcelo B. Labruna , Fernando de Castro Jacinavicius

{"title":"Diversity and pathogen surveillance in chigger mites across Brazil's five biogeographic regions","authors":"Isabella Pereira Pesenato , Ricardo Bassini-Silva , Ana Cláudia Calchi , Sueli Akemi Taniwaki Miyagi , Ester Nascimento da Costa , Carlos Cezar Trigo Nasser-Felix , Glauber Meneses Barboza de Oliveira , Mauricio Claudio Horta , Gabriela Cousandier , Jonas Sponchiado , Ralph Maturano , Graziela Virginia Tolesano-Pascoli , Renata D. Alquezar , Camile Lugarini , Ariane Ferreira , Luciano Moreira-Lima , Erika Hingst-Zaher , Josivania Soares Pereira , Darci Moraes Barros-Battesti , Marcelo B. Labruna , Fernando de Castro Jacinavicius","doi":"10.1016/j.parint.2026.103248","DOIUrl":"10.1016/j.parint.2026.103248","url":null,"abstract":"<div><div>The expansion of land use in Brazil has caused biodiversity loss and increased human interaction with parasites and pathogens previously restricted to natural ecosystems. Chigger mites are ectoparasites during the larval stage and can cause skin reactions or transmit pathogens to their hosts, including humans. This study aimed to evaluate the diversity of chiggers collected from all five Brazilian biogeographical regions, along with pathogen surveillance. Specimens were subjected to both morphological taxonomy and endogenous control, followed by PCR assays targeting pathogens of the genera <em>Borrelia</em>, <em>Orientia</em>, and <em>Rickettsia</em>. The analyses revealed a high species richness, including two novel records in Brazil: the genus <em>Boshkerria</em> and the species <em>Quadraseta antillarum</em>. Additionally, the genus <em>Quadraseta</em> tested positive for <em>Rickettsia</em> sp., while tests for the other agents were negative. This is the first report of a <em>Rickettsia</em> sp. agent detected in a <em>Quadraseta</em> nymph.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103248"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between serological tests and radiological imaging in the diagnosis of hydatid disease 血清学检查与影像学诊断包虫病的相关性研究

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-01-12 DOI: 10.1016/j.parint.2026.103231

Cemal Çiçek , Elif Tuğçe Güner , Rasime Pelin Kavak , Ayfer Bakır , Murat Aral

{"title":"Correlation between serological tests and radiological imaging in the diagnosis of hydatid disease","authors":"Cemal Çiçek , Elif Tuğçe Güner , Rasime Pelin Kavak , Ayfer Bakır , Murat Aral","doi":"10.1016/j.parint.2026.103231","DOIUrl":"10.1016/j.parint.2026.103231","url":null,"abstract":"<div><div>Hydatid disease (HD) is a zoonotic parasitic infection caused by the larval stage of <em>Echinococcus granulosus</em>. It remains a major public health issue in many countries, including Türkiye. Diagnosis relies on clinical findings, serological tests, and radiological methods. This study aimed to investigate the relationship between the indirect hemagglutination (IHA) test and radiological imaging in suspected HD cases. In this retrospective study, patients with suspected HD between July 2023 and June 2024 were included. Serum samples were tested using the IHA method, and ultrasonography (US), computed tomography (CT), or magnetic resonance imaging (MRI) was performed within 15 days for seropositive patients. The association between IHA titers and cyst size, localization, and the number of affected organs was analyzed. The study included 1289 cases, and seropositivity was detected in 147 (11.4%) of these cases. Seropositivity was higher in men (12.3%) than in women (10.7%). The majority of cases were in the 41–60 age group (43.5%). US was the most frequently used radiological imaging method, and the most common cyst location was the liver (91.95%). Although descriptive differences in cyst diameters were observed across different IHA titer levels, no statistically significant correlation was identified. These results indicate that IHA titers do not reliably reflect cyst size, disease extent, or radiological severity. Therefore, serological findings should not be interpreted as indicators of disease burden and must be evaluated strictly in conjunction with radiological imaging in the diagnosis, staging, and follow-up of hydatid disease.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103231"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145957774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of co-toxicity and synergistic action mechanism of essential oils derived from Citrus plants against Anopheles stephensi and Culex quinquefasciatus 柑橘精油对斯氏按蚊和致倦库蚊的共毒及协同作用机制评价。

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-01-23 DOI: 10.1016/j.parint.2026.103241

Shivani Sharma, Shweta Gupta, Mahima Chaudhary, Neha Loach, Lalit Mohan

{"title":"Assessment of co-toxicity and synergistic action mechanism of essential oils derived from Citrus plants against Anopheles stephensi and Culex quinquefasciatus","authors":"Shivani Sharma, Shweta Gupta, Mahima Chaudhary, Neha Loach, Lalit Mohan","doi":"10.1016/j.parint.2026.103241","DOIUrl":"10.1016/j.parint.2026.103241","url":null,"abstract":"<div><div>Mosquitoes have been claimed as the deadliest animals around the globe that cause severe fatal diseases and affect millions of people worldwide. Nowadays, it is well-understood that plant-based products can be used as alternatives to the synthetic insecticides to control a wide range of vectors and pests. The plant derived essential oils, the subject of considerable research and these natural bioactive compounds may replace synthetic insecticides in the consumer markets. Therefore, to enhance the larvicidal toxicity of essential oils, the combined bioassay of leaves and peels essential oils of <em>Citrus limetta</em>, <em>C. limon</em> and <em>C. reticulata</em> were evaluated by using WHO protocol. The findings of present study revealed that all the combinations demonstrated the synergistic effect with combined factor ranging from 1.02 to 1.34 and increased lethal values ranging from 2.09 to 33.90% against both the target species. The highest combined bioefficacy was achieved by CMLEO–CMPEO (1:1) with lowest LC<sub>50</sub> values, 12.18 and 21.85 ppm against <em>Anopheles stephensi</em> and <em>Culex quinquefasciatus</em>, respectively after 24 h of exposure. Hence, these different combinations of <em>Citrus</em> essential oils were proved as more toxic and have great capability for the development of a botanical insecticidal formulation to combat the mosquitoes' populations.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103241"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary regulation of peroxisome proliferator-activated receptors (α and γ) and IL-1R-associated kinase proteins in experimental Trypanosoma cruzi infection: Insights into one health 饮食对实验性克氏锥虫感染中过氧化物酶体增殖物激活受体（α和γ）和il - 1r相关激酶蛋白的调节：对一种健康的见解

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-02-01 DOI: 10.1016/j.parint.2026.103246

Débora Maria Soares de Souza , Maria Bárbara Galdino-Silva , Karine Maria Moreira Almeida , Maria Cláudia Silva , João Santana Silva , André Talvani

{"title":"Dietary regulation of peroxisome proliferator-activated receptors (α and γ) and IL-1R-associated kinase proteins in experimental Trypanosoma cruzi infection: Insights into one health","authors":"Débora Maria Soares de Souza , Maria Bárbara Galdino-Silva , Karine Maria Moreira Almeida , Maria Cláudia Silva , João Santana Silva , André Talvani","doi":"10.1016/j.parint.2026.103246","DOIUrl":"10.1016/j.parint.2026.103246","url":null,"abstract":"<div><div>Chagasic cardiomyopathy, caused by <em>Trypanosoma cruzi</em>, is influenced by host-parasite genetics and environmental factors such as diet. High-fat diets can intensify inflammation via pathways involving IRAK-1 and PPARs (α/γ). This study examined the impact of monounsaturated (olive oil) and saturated (lard) fat-rich diets on the expression of <em>IRAK-1</em>, <em>PPAR-α</em>, and <em>PPAR-γ</em> in cardiac and adipose tissues of mice infected with the Colombian strain of <em>T. cruzi</em>. Mice received control, olive oil, or lard diets during the experiment, and the infection was evaluated for 30 or 100 days. Infection increased <em>IRAK-1</em> expression in both tissues at 30 dpi and a cardiac reduction at 100 dpi. <em>PPAR-α</em> and <em>PPAR-γ</em> were suppressed at both time points, while PPARs were decreased in the presence of <em>T. cruzi</em>. In infected mice, fat-rich diets further decreased <em>PPAR-α</em> in cardiac tissue, while olive oil upregulated <em>PPAR-γ</em> in cardiac tissue (30 dpi), and lard did so with both PPARs in adipose tissues (100 dpi). These results highlight the phase-specific and fat-type-specific modulation of inflammatory pathways by lipid diets, underlining the potential role of nutrition in Chagas disease progression.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103246"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146113673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological evidence of direct hepatic invasion by Anisakis from the liver surface: A case report 异尖线虫从肝脏表面直接侵入肝脏的组织病理学证据：1例报告。

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-01-20 DOI: 10.1016/j.parint.2026.103244

Hiroki Takahashi , Reina Miyazawa , Mai Iwaya , Megumi Nobuoka , Tsuyoshi Terashima , Tsuyoshi Notake , Akira Shimizu , Yuji Soejima , Takeshi Uehara

{"title":"Histopathological evidence of direct hepatic invasion by Anisakis from the liver surface: A case report","authors":"Hiroki Takahashi , Reina Miyazawa , Mai Iwaya , Megumi Nobuoka , Tsuyoshi Terashima , Tsuyoshi Notake , Akira Shimizu , Yuji Soejima , Takeshi Uehara","doi":"10.1016/j.parint.2026.103244","DOIUrl":"10.1016/j.parint.2026.103244","url":null,"abstract":"<div><div><em>Anisakis</em> occasionally parasitizes extra-gastrointestinal organs, including the liver, pancreas, and ovary. We herein report a case of hepatic anisakiasis in which the route of hepatic invasion by <em>Anisakis</em> was histologically confirmed. A 50-year-old Japanese man with a history of left testicular seminoma underwent follow-up contrast-enhanced dynamic computed tomography, which revealed a liver mass suspicious for metastasis. The scan showed a low-attenuation area near the liver surface in segment 7. Laparoscopic partial hepatectomy was performed for diagnosis and treatment. Histologically, the mass showed necrosis and inflammatory infiltration, mainly by lymphocytes and neutrophils, with a parasite observed within the lesion. The parasite exhibited characteristic features—bifurcated Y-shaped lateral cords and a prominent renette cell—diagnostic of <em>Anisakis</em> larvae. A continuous linear band of granulation tissue connecting the liver surface to the lesion suggested the route of invasion. Retrospective imaging review confirmed that the hypodense linear band extending from the liver surface to the mass represented the direct invasion pathway. This case provides clear evidence of the hepatic invasion route in anisakiasis. Although rarely encountered, recognition of this feature may help distinguish hepatic anisakiasis from malignant liver tumors.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103244"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water buffaloes (Bubalus bubalis): potential reservoirs of trypanosomatids in endemic areas 水牛（Bubalus bubalis）：流行地区潜在的锥虫病宿主。

IF 1.9 4区医学

Parasitology International Pub Date : 2026-08-01 Epub Date: 2026-01-14 DOI: 10.1016/j.parint.2026.103242

Ana Paula Peres Lopes Romariz , Diogo Tiago da Silva , Julia Cristina Benassi , João Augusto Franco Leonel , Julio Cesar Pereira Spada , Carla Monadeli Filgueira Rodrigues , Herakles Antonio Garcia Pérez , Nathalia Frigo de Almeida Paula , Marta Maria Geraldes Teixeira , Trícia Maria Ferreira de Sousa Oliveira , Wilma Aparecida Starcke Buzetti

{"title":"Water buffaloes (Bubalus bubalis): potential reservoirs of trypanosomatids in endemic areas","authors":"Ana Paula Peres Lopes Romariz , Diogo Tiago da Silva , Julia Cristina Benassi , João Augusto Franco Leonel , Julio Cesar Pereira Spada , Carla Monadeli Filgueira Rodrigues , Herakles Antonio Garcia Pérez , Nathalia Frigo de Almeida Paula , Marta Maria Geraldes Teixeira , Trícia Maria Ferreira de Sousa Oliveira , Wilma Aparecida Starcke Buzetti","doi":"10.1016/j.parint.2026.103242","DOIUrl":"10.1016/j.parint.2026.103242","url":null,"abstract":"<div><div>Trypanosomatids are protozoan parasites that include the genera <em>Trypanosoma</em> and <em>Leishmania,</em> which infect a wide range of mammalian species, including humans and ruminants. This study aimed to assess the presence of Trypanosomatid parasites in water buffaloes (<em>Bubalus bubalis</em>) using molecular techniques. Blood samples and conjunctival swabs from the right and left eyes were collected from 100 buffaloes (44 females and 56 males). PCR analysis detected Trypanosomatids in 32% (32/100) of the buffaloes: 29% (29/100) tested positive for DNA extracted from blood, and 4% (4/100) tested positive from conjunctival swab samples. Using the Fluorescent Fragment Length Barcoding (FFLB) technique, 38% (38/100) of blood samples were positive for Trypanosomatids, with 35% (35/100) identified as <em>Trypanosoma theileri</em> and 3% (3/100) as <em>Trypanosoma vivax</em>. Direct sequencing and analysis of PCR amplicons from four buffaloes revealed that three samples matched 100% with <em>Trypanosoma theileri</em>, while one matched 100% with <em>Leishmania infantum</em>. Our findings confirm that buffaloes can serve as hosts for Trypanosomatids and support previous observations that these parasites are often underdiagnosed. This is the first report of <em>Leishmania infantum</em> DNA in buffalo conjunctival swabs in Brazil and the first detection of <em>Trypanosoma vivax</em> DNA in buffaloes in the city of Andradina and in São Paulo state. These findings underscore the need for further studies to clarify the role of buffaloes in the epidemiology and dissemination of trypanosomatids in livestock populations.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"113 ","pages":"Article 103242"},"PeriodicalIF":1.9,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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