Parasitology International最新文献

{"title":"Calicotyle confusa n. sp. (Monogenea: Monocotylidae) from Okamejei boesemani (Elasmobranchii) off Vietnam","authors":"Alika Kanaeva ,&nbsp;Ekaterina Vodiasova ,&nbsp;Vo Thi Ha ,&nbsp;Viktoria Uppe ,&nbsp;Evgenija Dmitrieva","doi":"10.1016/j.parint.2025.103100","DOIUrl":"10.1016/j.parint.2025.103100","url":null,"abstract":"<div><div>Monogeneans of the genus <em>Calicotyle</em> Diesing, 1850 were found in the cloaca of the Arafura skate <em>Okamejei boesemani</em> (Ishihara, 1987) from the coastal waters of Vietnam. A partial 28S rDNA sequence (822 bp) was obtained from the collected specimen and deposited in GenBank (accession number PP868284). Comparative analysis with the available 28S rDNA sequences of <em>Calicotyle</em> spp. in the NCBI database revealed the smallest p-distances with the <em>C. kroyeri</em> Diesing, 1850 species complex. However, the genetic distance between the obtained sequence and <em>C. kroyeri</em> (AF279746) from its type host, <em>Amblyraja radiata</em> (Donovan, 1808), and type locality (the North Sea), was 4.2 %, while distances from all <em>C. kroyeri</em> isolates from the Atlantic Ocean (AF279744 – AF279747) ranged from 4.2 to 5.9 %. Based on these results, a new species, <em>C. confusa</em> n. sp., was proposed. Morphologically, <em>C. confusa</em> n. sp. exhibits smaller, albeit overlapping, dimensions compared to <em>C. kroyeri</em> specimens from the North Atlantic. However, no significant differences were observed between the two species in taxonomically important characters. Further morphological studies of <em>C. kroyeri</em> from its type host, as well as from other hosts and regions, are required to identify potential diagnostic characters distinguishing it from <em>C. confusa</em> n. sp.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103100"},"PeriodicalIF":1.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biliary ascariasis in a non-endemic region: A case report","authors":"Raniah Alsubhi ,&nbsp;Noha Bakhsh","doi":"10.1016/j.parint.2025.103096","DOIUrl":"10.1016/j.parint.2025.103096","url":null,"abstract":"<div><div>Over one billion people worldwide are affected by <em>Ascaris lumbricoides</em>. While ascariasis is rare in Saudi Arabia, it is occasionally encountered among expatriates from endemic regions. We report a case of biliary ascariasis in a 29-year-old female expatriate, highlighting the clinical, laboratory, radiological, and endoscopic retrograde cholangiopancreatography (ERCP) findings, with particular emphasis on the diagnostic role of imaging. The patient presented to the emergency department with right upper quadrant abdominal pain, although physical examination was unremarkable. Abdominal ultrasound revealed diffuse dilatation of the intra- and extrahepatic biliary tree, with a long, mobile, echogenic structure within the common bile duct, lacking acoustic shadowing. Magnetic resonance cholangiopancreatography (MRCP) confirmed the presence of intraductal <em>Ascaris</em> extending into the intrahepatic ducts. The patient was started on antiparasitic therapy and had an uneventful recovery. This case underscores the essential role of imaging in diagnosing biliary ascariasis, particularly when clinical and laboratory findings are nonspecific.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103096"},"PeriodicalIF":1.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and morphological characterization, phylogenetic affinities and new records of Trypanorhyncha (Cestoda) from the Mexican Atlantic","authors":"Berenice Adán-Torres ,&nbsp;Luis García-Prieto ,&nbsp;Virginia León-Règagnon","doi":"10.1016/j.parint.2025.103095","DOIUrl":"10.1016/j.parint.2025.103095","url":null,"abstract":"<div><div>During a parasitological survey of elasmobranchs of the Atlantic Ocean on the Mexican coast, the digestive tract of some specimens of the southern stingray, <em>Hypanus americanus</em> (Hildebrand &amp; Schroeder, 1928), and the Atlantic sharpnose shark, <em>Rhizoprionodon terraenovae</em> (Richardson, 1836), were analyzed for tapeworms. These elasmobranchs were obtained from commercial fishing in four localities. Five taxa of Trypanorhyncha were recovered: <em>Kotorella pronosoma</em> (Stossich, 1901) Euzet &amp; Radujkovic, 1989, <em>Oncomegas wageneri</em> (Linton, 1890) Dollfus, 1929, <em>Pterobothrium kingstoni</em> Campbell &amp; Beveridge, 1996, and <em>Pterobothrium</em> sp., from <em>H. americanus</em>, and <em>Callitetrarhynchus gracilis</em> (Rudolphi, 1819) Pintner, 1931, from <em>R. terraenovae.</em> The morphology of collected parasite specimens was characterized based on light and scanning electron microscopy observations; sequences of nuclear large subunit (28S ribosomal DNA gene), nuclear small subunit (18S ribosomal DNA gene) and mitochondrial cytochrome <em>c</em> oxidase subunit I (COI) were obtained and used to infer their phylogenetic affinities. In the phylogenetic hypothesis presented herein (based on 18S rDNA and 28SrDNA), species of <em>Pterobothrium</em> do not form a monophyletic group, <em>K. pronosoma</em> appears to be a complex of cryptic species and their phylogenetic position remains controversial. Concerning <em>C. gracilis</em>, sequence divergence and phylogenetic position of the specimens collected in this study suggest the existence of more than one species. Finally, <em>O. wageneri</em> nested with all previous sequences generated for this species obtained from the Mexican Atlantic coast.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103095"},"PeriodicalIF":1.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new gallbladder parasite Ceratomyxa costaecola n. sp. (Cnidaria: Myxosporea) infecting the Goldblotch grouper Epinephelus costae (Serranidae) from Tunisian waters","authors":"Khouloud Bouderbala ,&nbsp;Luis F. Rangel ,&nbsp;Maria J. Santos ,&nbsp;Sihem Bahri","doi":"10.1016/j.parint.2025.103094","DOIUrl":"10.1016/j.parint.2025.103094","url":null,"abstract":"<div><div>A new species of <em>Ceratomyxa</em> Thélohan, 1892 named <em>Ceratomyxa costaecola</em> n. sp. was recorded in the gallbladder of the <em>Epinephelus costae</em> (Steindachner) fish found in the Gulf of Tunis, Tunisia. Plasmodia of this species were disporic, ellipsoidal in shape and measured between 13 and 16 μm in length and 20–24 μm in width. The mature myxospores were elongated, crescent-shaped with convex anterior and concave posterior angle, measuring 6.3 ± 0.5 (5.8–7.0) μm in length and 24.7 ± 2.3 (22.0–27.0) μm in thickness. Myxospores with two unequal shell valves, the longer one with a rounded end and the shorter one with a pointed end. Polar capsules were pyriform, equal in size, measuring 2.5 ± 0.1 (2.4–2.8) μm in length and 2.2 ± 0.1 (2.1–2.4) μm in width, with 4–5 coils of polar filament measuring 14.7–26.0 μm long after extrusion. The binucleated sporoplasm filled the entire cavity of the myxospore. The phylogenetic analysis showed that <em>C. costaecola</em> n. sp. was closely related to <em>Ceratomyxa</em> species infecting Epinephelidae fishes and also other fishes families from different localities.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103094"},"PeriodicalIF":1.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effect of new antimalarial N-89 for gametocytes in P. berghei-infected mice","authors":"Thi Quyen Dinh ,&nbsp;Hiroaki Matsumori ,&nbsp;Mamoru Niikura ,&nbsp;Shin-ichi Miyoshi ,&nbsp;Hye-Sook Kim","doi":"10.1016/j.parint.2025.103093","DOIUrl":"10.1016/j.parint.2025.103093","url":null,"abstract":"<div><div>We have developed a transdermal formulation of 1,2,6,7-tetraoxaspiro [7.11] nonadecane (td N-89) for children under the age of five, who account for the majority of malaria deaths, as multiple oral doses are difficult to administer to pediatric. Td N-89 shows promise as a new antimalarial candidate for targeting trophozoites using <em>Plasmodium berghei in vivo.</em> Primaquine (PQ) is currently the only drug that targets the liver stage parasites against <em>P. vivax</em> and <em>P. ovale.</em> When PQ is used in combination to target gametocytes, caution is required to prevent PQ resistance. To investigate the effect of td N-89 on gametocytes, we assess the gametocytocidal activity by measuring the expression levels of gametocyte-related genes following td N-89 treatment. The <em>P. berghei</em> ookinete surface protein (Pbs21) and generative cell-specific 1(GCS) were used as markers for female and male gametocytes. Expression levels of <span>Pbs21</span> and GCS increased during td N-89 at ED₉₀ dose. In mice with td artemisinin as a reference, <span>Pbs21</span> and GCS expression levels were higher than those in the td N-89 group, suggesting that td artemisinin exhibited higher anti-asexual parasite activity compared to td N-89. However, all mice treated with td artemisinin showed recrudescence and died. In contrast, 33.3 % of mice treated with td N-89 achieved a cure despite 1.7 % parasitemia. We report for the first time that gametocyte-related genes expression progressively changes in the td N-89 group and can be detected for a long period. Taken together, our results suggest that target of td N-89 is mainly trophozoites and not gametocytes.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103093"},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of Brachylaima asakawai (Trematoda: Brachylaimidae) in a carnivore host, the red fox (Vulpes vulpes schrencki)","authors":"Masahito Hidaka ,&nbsp;Kohji Uraguchi ,&nbsp;Hiroyuki Matsuyama ,&nbsp;Hirokazu Kouguchi","doi":"10.1016/j.parint.2025.103085","DOIUrl":"10.1016/j.parint.2025.103085","url":null,"abstract":"<div><div>Accumulating information on the relationship between hosts and parasites is important to understand their life cycle. Nine <em>Brachylaima</em> spp. species exist in Japan, but their life cycles are not fully understood, partly because of their low prevalence in their definitive hosts. In this study, 23 trematodes, thought to belong to the genus <em>Brachylyma</em> were found in the small intestine of the red fox (<em>Vulpes vulpes schrencki</em>) in Hokkaido, Japan. We analyzed these trematodes using morphological and molecular methods. Morphological analysis showed that the trematodes had the same features as <em>Brachylaima asakawai</em>. We extracted DNA from the trematodes and amplified the nuclear 28S ribosomal RNA (28S rDNA) and mitochondrial cytochrome <em>c</em> oxidase subunit 1 (<em>cox</em>1) genes. The 28S rDNA and <em>cox</em>1 fragments were 100 % and 99.7 % identical to the <em>B. asakawai</em>, respectively. Previously, <em>B. asakawai</em> was detected only in rodents in Hokkaido; however, this study demonstrated that it is also a fox parasite. This is the second report of a definitive natural host for <em>B. asakawai</em> and the first record of the red fox being found as a new definitive host for <em>B. asakawai</em>. To fully understand the host preference and range of <em>B. asakawai</em>, it is essential to continuously survey various animals to understand the life cycle of <em>B. asakawai</em> completely.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103085"},"PeriodicalIF":1.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimalarial drug efficacy and resistance: Insights from northern Uganda","authors":"Toshihiro Mita","doi":"10.1016/j.parint.2025.103083","DOIUrl":"10.1016/j.parint.2025.103083","url":null,"abstract":"<div><div>Artemisinin in combination is the first-line treatment for <em>Plasmodium falciparum</em> malaria in almost all regions. However, by the late 2000s, partial resistance, characterized by delayed parasite clearance after treatment, emerged in the Greater Mekong Subregion and rapidly expanded its range. Since 2013, we have conducted comprehensive malaria drug resistance surveillance in northern Uganda. In 2014, we identified <em>in vitro</em> artemisinin resistance, and by 2017, clinical partial resistance had also been observed. Additionally, we discovered the re-emergence of chloroquine sensitivity in this region as early as 2013, earlier than in the other parts of Uganda. This review aims to summarize our findings from northern Uganda, contextualizing them within similar studies conducted in other regions.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103083"},"PeriodicalIF":1.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and forecasting of cysticercosis burden in South Asia: Insights from global burden of disease data (1990–2021)","authors":"Ayush Sharma ,&nbsp;Diptismita Jena ,&nbsp;Muhammed Shabil ,&nbsp;Mahalaqua Nazli Khatib ,&nbsp;Mandeep Kaur ,&nbsp;Shriya Bhatnagar ,&nbsp;Manish Srivastava ,&nbsp;Pranchal Rajput ,&nbsp;Abhay M. Gaidhane ,&nbsp;Amit Verma ,&nbsp;Chanchal Goyal ,&nbsp;Abhishek Mewara","doi":"10.1016/j.parint.2025.103092","DOIUrl":"10.1016/j.parint.2025.103092","url":null,"abstract":"<div><div>Cysticercosis, caused by <em>Taenia solium</em> larvae, is a neglected tropical disease endemic in more than 75 countries of the world. It significantly contributes to epilepsy in regions with poor sanitation and inadequate food safety practices. This study aims to examine the trends in cysticercosis burden in South Asia (SA) from 1990 to 2021 using prevalence and disability-adjusted life years (DALYs) rates, and to forecast the disease burden up to 2031. Data from Global Burden of Disease (GBD) (1990–2021) was utilized to estimate DALYs and prevalence rates. Trend analysis was done by Joinpoint regression to detect temporal changes in cysticercosis. Future burden projections used the Autoregressive Integrated Moving Average (ARIMA) model, forecasting through 2031. The highest prevalence rates of cysticercosis per 100,000 population in the SEA region were recorded in Nepal, followed by Bhutan, and India, however, the trends differed from country to country. There was marked reduction in the disease burden from 1990 to 2021 across SA region, with India and Nepal achieving the highest numerical reductions in prevalence (47.38 and 39.91 respectively). Forecasting suggests a consistent overall reduction in both prevalence (50.55 to 42.14), and DALYs (14.35 to 10.58) from 2022 to 2031 respectively in the SA region. Despite an overall reduction in prevalence and DALYs, disparities in the cysticercosis burden in the different SA countries were observed. The forecasting predicted that though cysticercosis burden is expected to reduce, but it is likely to remain a major public health problem. Thus, the control of the disease will need continued efforts to enhance sanitation, health education, and pig husbandry management, integrated into the national health systems of the countries.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103092"},"PeriodicalIF":1.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine intestinal pseudomyiasis by blow flies and first documented case report caused by Lucilia cuprina in Central America","authors":"Sabrina Lazo-Láscarez ,&nbsp;Irene Alvarado-Hidalgo ,&nbsp;Diana Rojas-Araya ,&nbsp;Adriana Troyo ,&nbsp;Olger Calderón-Arguedas","doi":"10.1016/j.parint.2025.103084","DOIUrl":"10.1016/j.parint.2025.103084","url":null,"abstract":"<div><div>Pseudomyiases occur when vertebrates ingest eggs or larvae of flies that survive gastrointestinal passage and may result in pathological reactions, but they are usually unable to continue their life cycle in the host. Many blow fly species (Diptera: Calliphoridae) are associated with facultative cutaneous myiasis in humans and animals; however, very few cases of blow fly pseudomyiasis have been published in animals. In this report, two cases of canine pseudomyiasis caused by blow flies are documented, and <em>Lucilia cuprina</em> Wiedemann was confirmed as the species responsible in one of them. It is likely that cases of pseudomyiasis in dogs are being underestimated, so it is important to consider and identify these events as a differential diagnosis of gastrointestinal disease to prevent recurrences. Recognizing the particularities of blow fly behavior should be a priority for sanitary authorities to establish control strategies and prevent their impact on public health.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103084"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemotaxis of Miamiensis avidus to hyaluronic acid, a component of fish surface mucus","authors":"Sang-Hee Kim ,&nbsp;Tadashi Isshiki ,&nbsp;Shin-Ichi Kitamura","doi":"10.1016/j.parint.2025.103082","DOIUrl":"10.1016/j.parint.2025.103082","url":null,"abstract":"<div><div><em>Miamiensis avidus</em> is a marine parasitic ciliate belonging to the order Scuticociliatida, the members of which are the causal agents of scuticociliatosis. This ciliate has resulted in considerable economic losses to the aquaculture industry in the Republic of Korea and Japan. Nevertheless, the development of vaccines and therapeutics has proven to be challenging, and there are currently no reports of such products commercially available in Korea or Japan. Furthermore, the aetiology and pathogenesis of <em>M. avidus</em> infection in fish remain unknown, as do the underlying reasons for the initial infection. Our goal was to investigate the mechanism of infection by examining the chemotactic response of <em>M. avidus</em> to fish tissues. We found that <em>M. avidus</em> exhibited a high degree of chemotaxis towards the tissues, blood, and surface mucus of the olive flounder (<em>Paralichthys olivaceus</em>) specimens used in the experiment. We analyzed the components of olive flounder surface mucus to determine the degree of chemotaxis induced by each component. <em>Miamiensis avidus</em> exhibited pronounced chemosensitivity to hyaluronic acid, one of the constituents. Given the nature of the surface mucus, it is plausible to suggest that it acts as a potent attractant for initial parasitism by scuticociliates.</div></div>","PeriodicalId":19983,"journal":{"name":"Parasitology International","volume":"109 ","pages":"Article 103082"},"PeriodicalIF":1.5,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}