Simple preservation of schistosome eggs with high infectivity up to 12 weeks

IF 1.5 4区 医学 Q3 PARASITOLOGY
Yukiteru Ouji , Megumi Hamasaki , Masayasu Misu , Masahide Yoshikawa , Shinjiro Hamano
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引用次数: 0

Abstract

The lifecycle of schistosomes must be continuously maintained to clarify and understand this parasite in various aspects in laboratory settings. In the previous studies by other researchers, preservation of schistosome larvae or eggs was attempted by freezing with liquid nitrogen or organic chemicals, but frozen schistosomes were substantially impaired. The present study was conducted to determine whether schistosome eggs can be preserved under a non-frozen condition. The results showed that Schistosoma mansoni eggs could be maintained in phosphate-buffered saline at 4 °C, with a high level of infectivity of miracidia to freshwater snails thereafter. Furthermore, the egg hatchability was maintained for up to 12 weeks with weekly exchanges of the medium. The cercariae derived from snails infected with miracidia from preserved eggs were highly infective to mice. This simple schistosome egg preservation method allow researchers to maintain the schistosome lifecycle without freezing or other special procedures.

Abstract Image

具有高传染性的血吸虫卵可简单保存12 周。
必须持续维持血吸虫的生命周期,以便在实验室环境中从各个方面阐明和了解这种寄生虫。在其他研究人员之前的研究中,曾尝试用液氮或有机化学品冷冻保存血吸虫幼虫或卵,但冷冻后的血吸虫受到严重损害。本研究旨在确定血吸虫卵能否在非冷冻条件下保存。结果表明,在4 °C的磷酸盐缓冲盐水中,曼氏血吸虫卵可以维持,此后miracidia对淡水蜗牛具有较高的传染性。此外,在每周更换培养基的情况下,鸡蛋的孵化率可维持12 周。钉螺尾蚴感染了皮蛋中的miracidia,对小鼠具有高度传染性。这种简单的血吸虫卵保存方法使研究人员无需冷冻或其他特殊程序即可维持血吸虫的生命周期。
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来源期刊
Parasitology International
Parasitology International 医学-寄生虫学
CiteScore
4.00
自引率
10.50%
发文量
140
审稿时长
61 days
期刊介绍: Parasitology International provides a medium for rapid, carefully reviewed publications in the field of human and animal parasitology. Original papers, rapid communications, and original case reports from all geographical areas and covering all parasitological disciplines, including structure, immunology, cell biology, biochemistry, molecular biology, and systematics, may be submitted. Reviews on recent developments are invited regularly, but suggestions in this respect are welcome. Letters to the Editor commenting on any aspect of the Journal are also welcome.
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