Parasitology Research最新文献

{"title":"Microtetrameres cloacitectus in Eurasian buzzard (Buteo buteo): pathology, phylogenetics, and seasonality.","authors":"Katarzyna Anna Hołówka, Andrada Negoescu, Marian Taulescu, Angela Monica Ionică, Georgiana Deak, Andrei Daniel Mihalca, Călin Mircea Gherman","doi":"10.1007/s00436-025-08517-w","DOIUrl":"10.1007/s00436-025-08517-w","url":null,"abstract":"<p><p>Microtetrameres cloacitectus is a nematode of the family Tetrameridae Travassos, 1914, parasitising the proventricular glands of diurnal raptors. First described by Oschmarin (1956) from the Eurasian buzzard (Buteo buteo) in Primorsky Krai, Russia, it has since been reported in several raptor species. Between 2017 and 2024, 88 deceased Eurasian buzzards were collected from various regions in Romania. For each specimen, GPS coordinates, sex, and age were recorded, and full parasitological necropsies were performed. One individual (over three-year-old male found as roadkill in Tureni, Cluj County, Northwestern Romania) had Microtetrameres-like nematodes; a section of its proventriculus was preserved in 10% formalin for histopathological examination. In the remaining positive cases, nematodes were preserved in ethanol for molecular analyses and in 4% formalin for morphological identification. Overall, 25 buzzards were infected with M. cloacitectus, resulting in a prevalence of 28.4% (95% CI 20.0-38.6%). A total of 779 specimens (464♀, 315♂) were recovered, with an intensity range of 1-316 parasites (mean = 31.2). Infections showed a seasonal pattern, peaking in summer, and were more frequent in birds over 2 years old. Molecular analysis yielded a novel cox1 sequence and revealed new phylogenetic relationships. Histopathology revealed multifocal glandular distention and inflammatory nodules centred on parasites. This study provides the first comprehensive parasitological, molecular, and histopathological characterisation of M. cloacitectus in Romania.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"74"},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated diagnosis and successful treatment of imported old world cutaneous leishmaniasis with intralesional sodium stibogluconate in china: a case report.","authors":"Junzhu Xu, Xiujiao Xia, Zehu Liu","doi":"10.1007/s00436-025-08524-x","DOIUrl":"10.1007/s00436-025-08524-x","url":null,"abstract":"<p><p>This study presents the case of a 54-year-old woman, otherwise healthy, who presented with a 4-month history of red plaques with central ulceration, which had initially developed from painless papules. The lesions appeared on her right arm and trunk, two months after her travel to Morocco. Physical examination revealed a 7 cm × 5 cm red plaque with central ulceration and crusting on her right arm, along with papules and plaques on her trunk. Based on microbiological, histopathological, and PCR results, the patient was diagnosed with Old World cutaneous leishmaniasis. After receiving two sessions of intralesional pentavalent antimonial treatment, the patient was left with only a minor scar and experienced negligible side effects, except for moderate aching during therapy.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"75"},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of parasitic fauna of three species of deer occurring in southeastern Mexico.","authors":"Brenda Solórzano García, Gerardo Pérez Ponce de León","doi":"10.1007/s00436-025-08518-9","DOIUrl":"10.1007/s00436-025-08518-9","url":null,"abstract":"<p><p>Three species of deer can be found in southeastern Mexico, the white-tailed deer (Odocoileus virginanus), the red brocket deer (Mazama temama), and the Yucatan brocket deer (O. pandora). Here, we applied a combination of non-invasive sampling, necropsy examinations, and molecular diagnostics to assess the endoparasite infections fauna of in wild and captive populations of these three deer species. We analyzed a total of 140 samples and found eleven taxa parasitizing these hosts, including two trematodes (Paramphistomum sp., an unidentified trematode), one anoplocephalid cestode, six nematodes (Strongyloides sp., two morphotypes of strongylid eggs, Mammomonogamus sp., Setaria sp. and an unidentified ascarid), and two protozoans (Giardia intestinalis and unidentified ciliates). The Yucatan brocket deer showed the highest percentage of infected samples (39.2%), followed by the white-tailed deer (38.7%). For the red brocket deer, only two samples were positive to two parasite taxa (20%). Captive populations showed the lowest parasite richness and percentage of infection. Some of the parasites reported here can pose potential risk for the health of deer populations, and along with habitat loss and poaching, could jeopardize the persistence of deer in the area. This study adds to the knowledge gap on the parasitic fauna in wild populations of these culturally and ecologically important species, particularly for the Yucatan brocket deer for which parasitological information is very scarce, and highlights the necessity to keep monitoring the health status of deer in this area.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"72"},"PeriodicalIF":2.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12209024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanopore sequencing reveals a diversity of microorganisms in ticks from Ethiopia.","authors":"Electra F Chadd, Koray Ergunay, Bersissa Kumsa, Brian P Bourke, Ben S Broomfield, Lewis S Long, Yvonne-Marie Linton","doi":"10.1007/s00436-025-08520-1","DOIUrl":"10.1007/s00436-025-08520-1","url":null,"abstract":"<p><p>Ethiopia is home to a diverse group of ixodid ticks that are known to transmit disease to both animals and humans. Recent advances in metagenome sequencing show there is more microorganism diversity found in ticks than previously known. Compared to amplicon-based gene identification methods, agnostic metagenomic sequencing provides broader insights into the diversity of microorganisms in ticks, providing knowledge that will better enable public health measures in preventing the spread of zoonotic disease. In the present study, metagenomic sequencing was used to look at the microbial diversity of ticks collected from livestock in Ethiopia. This study identified six bacterial genera (Coxiella, Francisella, spotted-fever group (SFG) Rickettsia, Spiroplasma, Ehrlichia, and Borrelia), one genus of eukaryotic parasite (Babesia sp.), and one virus species (Parapoxvirus bovinestomatitis) from 154 tick pools representing 22 species of ticks among four genera (Amblyomma, Haemaphysalis, Hyalomma, and Rhipicephalus). We were able to differentiate between pathogenic and nonpathogenic microorganisms, highlighting concerns among traditional gene-targeted screening methods. Among all pooled samples, the predominant microorganisms included Coxiella-like endosymbionts (55.2%), SFG Rickettsia (38.3%), and nonpathogenic Francisella spp. (26.0%). Rickettsia africae was the predominant pathogenic agent detected, and phylogenetic analysis of two samples from A. gemma and A. variegatum confirmed the presence of R. africae. This study highlights the power of metagenomics applied to potential vectors of zoonotic disease, and it expands the knowledge on tick-pathogen associations in Ethiopia.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"73"},"PeriodicalIF":2.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12209015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of two commonly and limited used chemosterilants on Lucilia sericata egg surface sterilization.","authors":"Nevra Polat, Salih Mollahaliloglu, Murat Koc","doi":"10.1007/s00436-025-08515-y","DOIUrl":"10.1007/s00436-025-08515-y","url":null,"abstract":"<p><p>Maggot therapy (MT) is the most common medical use of sterile fly larvae of Lucilia sericata and other species of the family Calliphoridae as an alternative to surgery and long-term antiseptic therapy in the treatment of deep and infected non-healing wounds. Effective and reliable MT requires an aseptic technique to prevent bacterial infection of the wound. However, due to the habitat of Calliphoridae flies, the outer surface of their eggs is often contaminated with bacteria. In this study, Lucilia sericata eggs were sterilized using two techniques. NaOCI (sodium hypochlorite), which is widely used as a sterilant (Sterilant 1-S1), and Lysol surface disinfectant, which is rarely used (Sterilant 2-S2), and a control (unsterilized) were chosen. Sterilization efficacy, egg survival, and mortality were evaluated comparatively at 0.05, 1, 2, and 3 (%) concentrations and 1-, 2-, 3-, and 4-min application times at each concentration, and bacterial growth in the post-sterilization environment was controlled. In the in vitro sterility efficiency and reliability test, no bacterial colonies were found in sterilized eggs transferred to tryptic soy agar (TSA) culture. In contrast, bacterial and fungal growth was detected in unsterilized eggs. Protocol S1 provided effective egg surface sterilization at a concentration of 0.05% with a 1-min exposure time. This treatment resulted in 85.12% survival rate and 14.88% egg mortality. Based on its optimal balance of low mortality, high viability, and effective sterilization, we recommend a 1-min immersion in 0.05% NaOCl solution for sterilization of Lucilia sericata eggs.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"70"},"PeriodicalIF":1.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Sarcocystis spp. infection in goats: a combined morphological, serological, and molecular approach.","authors":"K D Steffen, M L Gos, E Helman, J M Unzaga, R O Arias, G Moré","doi":"10.1007/s00436-025-08519-8","DOIUrl":"10.1007/s00436-025-08519-8","url":null,"abstract":"<p><p>Domestic goats (Capra aegagrus hircus) are intermediate hosts for three species of Sarcocystis: S. capracanis, S. hircicanis, and S. moulei. The present study aimed to describe and identify Sarcocystis spp. in goat/kid muscles utilizing optical and transmission electron microscopy, a serological method, and PCR-sequencing of individual cysts. Both S. capracanis and S. hircicanis were identified by light and transmission electron microscopy and molecular methods. No macroscopic cysts were detected in any of the examined animals (n = 90, from two Argentinean provinces); only microscopic sarcocysts of Sarcocystis spp. were detected in 17 of 19 adult goats (90%), but in no kids (n = 71) aged 37 to 290 days. Fourteen 18S rRNA and the six coxI sequences from individual cysts highlighted high similarity (99.06-100% and 98.89-99.81%, respectively) with S. capracanis sequences from other regions. Two distinct groups of S. capracanis 18S rRNA sequences with high intra-group identities (99.42-100%) were identified, as well as another sequence that did not group. Each group was integrated from samples of both sampled provinces. Despite relatively extensive differences between these groups of sequences (up to 3.8%), the phylogenetic analyses positioned the three representative sequences all together in a branch with S. capracanis sequences but also containing S. tenella. In addition, one 18S rRNA sequence showed a 100% identity with those of S. hircicanis. Two animals harbor S. capracanis and S. hircicanis cysts. Twelve out of 18 adult goats were seropositive by Sarcocystis spp. IFAT with titers ≤ 100. This is the first report that confirmed the infection and co-infection of S. capracanis and S. hircicanis in domestic goats from Argentina and evidenced a high variability in the 18S rRNA fragment sequences of S. capracanis.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"71"},"PeriodicalIF":1.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First complete mitochondrial genome of Armillifer moniliformis (Pentastomida: Porocephalida) isolated from a human case in Northern Thailand: comparative and phylogenetic analyses.","authors":"Thanapat Pataradool, Padet Siriyasatien, Woraporn Sukhumavasi, Saruda Tiwananthagorn, Chusana Suankratay, Kanok Preativatanyou","doi":"10.1007/s00436-025-08516-x","DOIUrl":"10.1007/s00436-025-08516-x","url":null,"abstract":"<p><p>Pentastomiasis, a zoonotic parasitic disease caused by pentastomids, has been increasingly documented in Thailand. Historically, morphological identification has been the only diagnostic method, but it is inadequate due to morphological similarities between larval stages and limited taxonomic keys. To overcome this, molecular diagnosis is required. However, the DNA sequence information of these neglected parasites remains largely unknown. In this study, pentastomid larvae were collected from the greater omentum of a patient in Northern Thailand. The species of pentastomid larvae was molecularly identified as Armillifer moniliformis, using PCRs targeting nuclear and mitochondrial regions, followed by Sanger sequencing. Nanopore sequencing was employed to characterize the complete mitochondrial genome of A. moniliformis, revealing a total length of 16,367 bp with 37 mitochondrial genes, including 13 protein-coding genes, 22 tRNA genes, and two rRNA genes, as well as one non-coding region. The entire mitogenome is highly AT-rich (62.1%), with nucleotide skews indicating compositional bias between strands. The non-coding region (2616 bp) lies between the tRNA-Leu^CUN and tRNA-Ser^UCN genes and comprises three repetitive segments. The third repetitive segment is the longest (1659 bp) and has the highest A + T content of 72.2%, likely serving as a regulatory element related to DNA replication. Comparative mitogenome analysis revealed cox1 as the most conserved gene, while atp6, atp8, and nad6 exhibit high variability among species. Codon usage analysis indicated that two codon families (Leu1 and Ile) are used more frequently than others, suggesting that these branched-chain hydrophobic amino acids are predominant in transmembrane proteins and underscoring the critical role of the mitochondrion in energy production. Based on single gene (cox1) and concatenated gene-based phylogenetic approaches, A. moniliformis clustered closely with other Armillifer species, with all pentastomids placed taxonomically in the subphylum Crustacea. Mitogenomic phylogenetics in this study demonstrates greater topological precision than cox1 phylogenetics. In conclusion, this study revealed the first complete mitochondrial genome of A. moniliformis, providing us with novel molecular markers that could effectively facilitate species diagnosis, treatment monitoring, and epidemiological surveillance of pentastomiasis.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"69"},"PeriodicalIF":1.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of cytochrome P450 and esterase genes in pyrethroid-resistant wild Culex quinquefasciatus populations from sub-Himalayan West Bengal, India.","authors":"Abhirup Saha, Prapti Das, Subhajit Das, Dhiraj Saha","doi":"10.1007/s00436-025-08487-z","DOIUrl":"10.1007/s00436-025-08487-z","url":null,"abstract":"<p><p>Culex quinquefasciatus Say (Diptera: Culicidae) is an important vector that spreads a number of diseases like lymphatic filariasis, Rift Valley fever, West Nile fever, Zika virus, and Saint Louis encephalitis. Due to long-term exposure to insecticides applied during vector control programs, these mosquito populations exhibit varying degrees of resistance in response to distinct selection pressures. Therefore, it is imperative to assess their resistance status before deploying any control programs. The present study examined the resistance status of wild Cx. quinquefasciatus mosquito larvae against two commonly used insecticides (cyphenothrin and temephos) from sub-Himalayan West Bengal. This study also examined the activity of carboxylesterases and the levels of cytochrome P450 enzymes in wild larvae of Cx. quinquefasciatus mosquitoes from three districts in the northern part of West Bengal. The expression pattern of selected P450 and esterase genes (Est A, Est B, CYP6AA7, CYP9J40, CYP9J45) has been assessed to investigate their involvement in metabolic resistance in this mosquito species. Most of the populations were fully resistant to cyphenothrin, whereas few were resistant to temephos. Significantly high activities and levels of targeted detoxifying enzymes were observed among all the wild populations. Overexpression of the Est B, CYP6AA7, and CYP9J40 genes was observed in most wild populations compared to susceptible ones. From this study, it can be suggested that metabolic resistance has an important role in developing resistance among Cx. quinquefasciatus populations of this region. Proper mapping of insecticide resistance should be done before using any insecticides.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"68"},"PeriodicalIF":1.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China.","authors":"Wei-Gang Chen, Li Li, Guo-Dong Dai, Yao-Dong Wu, Nigus Abebe Shumuye, Tharheer Oluwashola Amuda, Xue-Qi Tian, Sheng-Zhi Gao, Xue-Yi Hou, Nian-Zhang Zhang, Wen-Jun Tian, Lin-Sheng Zhang, Le Li, Fu-Heng Zhang, Min-Kuan Guo, Wen-Dong Li, Zhonggecao, Zhaxilamao, Gazangzhuoma, Doukao, Tao Yang, Bao-Quan Fu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia","doi":"10.1007/s00436-025-08494-0","DOIUrl":"10.1007/s00436-025-08494-0","url":null,"abstract":"<p><p>Echinococcosis is a severe parasitic zoonosis worldwide distribution, caused by the genus Echinococcus. One of its recently identified species, Echinococcus shiquicus, is believed to be endemic to the Qinghai-Tibet plateau in China. Research has confirmed that Tibetan foxes are the definitive hosts for E. shiquicus, while plateau pikas (Ochotona curzoniae) serve as intermediate hosts. In this study, cyst containing protoscolices was first observed in the lung of plateau zokor (Eospalax baileyi) in the Tibetan Autonomous Prefecture of Gannan, Gansu Province, China. This observation was confirmed using complete sequences of the cox1 and nad1 genes. We also characterized the cysts and examined the genetic diversity of Echinococcus shiquicus collected from the Qinghai-Tibet Plateau region, which includes Gansu Province, Qinghai Province, and the Tibet Autonomous Region. Phylogenetic trees and haplotype analyses for the isolates were constructed using the maximum likelihood method in MEGA 7.0.26 and DnaSP v6 software, based on the complete cox1 (1608 bp) and nad1 (897 bp) gene sequences, as well as a concatenated dataset of cox1-nad1 (2505 bp). The cyst containing protoscolices in the lung of plateau zokor was identified as Echinococcus shiquicus. The prevalence of E. shiquicus infection in plateau pikas in Luqu and Maqu counties in Gansu province was found to be 2.38% (3/126) and 3.36% (10/298), respectively, while in plateau zokors, it was 1.61% (1/62). Haplotype analysis, incorporating sequences from previous studies, revealed the presence of 17 haplotypes, indicating significant haplotype diversity in the study areas. The neutrality indices were Tajima's D =  - 1.438 (P > 0.05) and Fu's Fs =  - 3.169 (P > 0.05). This study provides important insights into Echinococcus shiquicus and its associations with plateau zokors and plateau pikas in the Qinghai-Tibet Plateau. We identified plateau zokors as a new natural intermediate host for E. shiquicus, emphasizing the complexity of its transmission dynamics and host interactions within this unique ecosystem. The observed morphological and developmental state differences in cysts between the liver and lung suggest that presence of tissue specificity in different organ. Additionally, the analysis of genetic diversity revealed high intraspecific variability, indicating a complex population structure influenced by various ecological factors.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"66"},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complete mitogenome of Hyalomma anatolicum: novel insights into the phylogenetics of Hyalomma.","authors":"Jin Che, Yijun Chai, Wei Li, Shuaiyang Zhao, Jin Luo, Qiaoyun Ren, Qingli Niu, Guiquan Guan, Jinming Wang, Hong Yin","doi":"10.1007/s00436-025-08497-x","DOIUrl":"10.1007/s00436-025-08497-x","url":null,"abstract":"<p><p>Ticks are obligate blood-sucking ectoparasite and common vector of a wide variety of zoonotic pathogens. In this research, we sequenced and assembled the circular complete mitogenome information of Chinese isolates of Hyalomma anatolicum maintained at the Laboratory. The 14,714 bp mitogenome consists of 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes and 2 ribosomal RNA (rRNA). The nucleotide sequence of Hy. anatolicum mitogenome includes approximately A (35.05%), G (8.70%), T (41.20%) and C (11.26%), the proportion of A + T (80.04%) is substantially higher than G + C (19.96%). We compared mitogenome selection pressure of the Hy. anatolicum and found that positive selection signatures were detected in atp8, contrasting sharply with the strong purifying selection observed in cox1. Phylogenomic reconstruction using concatenated PCGs robustly placed Hy. anatolicum within a clade sister to Hy. excavatum. We further validated cox3, cytb, nad1, nad2, nad3, nad4, nad4L, nad5 and nad6 as lineage-specific markers mirroring full mitogenome topology, proposing their cost-effective use in Hyalomma systematics. The divergence time analysis suggests that Hy. anatolicum was the most recent species in Hyalomma, 1.95 Mya. As far as we know, this is the first study exploring the complete mitogenome for Hy. anatolicum. Here, we obtained the whole mitogenome sequence for the species Hy. anatolicum, which enriched the mitogenome data of ticks, provided new idea for future research on the molecular evolution, population heredity and systematics of ticks.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 6","pages":"67"},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}