Parasitology Research最新文献

{"title":"Tick-borne pathogens in meat sheep from Brazil: first report of Theileria ovis in sheep in latin America.","authors":"Mário Felipe Alvarez Balaro, Matheus Dias Cordeiro, Isadora de Fátima Braga Magalhães, Júlia Cardoso Pereira, Bruna de Azevedo Baêta, Alexandro Guterres, Cláudia Bezerra da Silva, Nathalie Costa da Cunha","doi":"10.1007/s00436-025-08587-w","DOIUrl":"10.1007/s00436-025-08587-w","url":null,"abstract":"<p><p>Tick-borne hemoparasitic diseases have a worldwide presence, impacting public health and livestock economies. In this study, 16 ewes from the same farm were evaluated, 56% (9/16) exhibited various clinical symptoms, such as conjunctivitis, nasal discharge, cough, swollen lymph nodes, fever, and tick infestation. After physical examination, ultrasound and complete blood count analysis were performed, along with molecular testing using Polymerase Chain Reaction (PCR) and Sanger sequencing. Neutrophilic leukocytosis, mild normocytic normochromic anemia, thrombocytosis, reactive lymphocytes, and intra-cytoplasmic lymphocytic inclusion bodies were all identified. Spleen and liver abnormalities were confirmed by ultrasound. In total, 44% (7/16) of ewes were infested with ticks, which were identified as Dermacentor nitens, Rhipicephalus microplus, and Amblyomma sculptum. Of the 16 sheep screened for tick-borne agents using PCR, 37% (6/16) tested positive for the Piroplasmida order. Among these, 33% (2/6) showed co-infection with Anaplasma sp. None of the samples tested positive for Ehrlichia spp. or Borrelia spp. Molecular analyses, followed by sequence comparison, revealed the pathogens to be Theileria ovis and Anaplasma sp. closely related to Candidatus Anaplasma boleense. Ewes were treated with three doses of oxytetracycline (20 mg/kg) 48 h apart. After treatment, new blood smears and whole blood samples were negative for cell inclusions, and all PCR results were negative. The study identified a tick-borne disease caused by piroplasms and Anaplasma spp. in a Brazilian sheep flock with clinical remission following treatment. Furthermore, this research marks the first documentation of T. ovis and Anaplasma sp. closely related to Candidatus A. boleense infecting sheep in Latin America, emphasizing the need for further studies and surveillance of tick-borne pathogens in Brazil.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12953417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report of primary amebic meningoencephalitis in a Chinese adult male.","authors":"Ye Kuang, Qingyun Li, Hongmei Li, Yangfan Guo, Jia Wang, Chuanmei Peng, Pei He, Sulian Chen, Shiyan Nian, Lei Feng","doi":"10.1007/s00436-026-08647-9","DOIUrl":"10.1007/s00436-026-08647-9","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12945971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-level variation and molecular identification of dual Microphallus infections in shore crabs (Carcinus maenas).","authors":"Alexander T Bedford, Grace N Crocker, Andrew F Rowley, Charlotte E Davies","doi":"10.1007/s00436-025-08614-w","DOIUrl":"10.1007/s00436-025-08614-w","url":null,"abstract":"<p><p>Digenean trematodes commonly use marine crustaceans as intermediate hosts, yet their distribution and host-specific dynamics remain underexplored in temperate European systems. In this study, metacercarial stages of Microphallus similis and M. primas were identified in the hepatopancreas of Carcinus maenas collected from two distinct locations in South Wales, UK across a 4-month period. M. similis exhibited consistently high prevalence (~ 76%) at both sites, while M. primas occurred only as a co-infection, with lower prevalence (2-9%) and infection intensity. At Oxwich Bay, crab size and coloration were significantly associated with trematode infection, whereas no such associations were observed at Mumbles Head. Phylogenetic analysis confirmed species-level divergence between M. similis and M. primas. Overall parasite burden was significantly higher at Mumbles Head, with larger crabs harbouring more metacercariae. Additionally, metacercariae from Mumbles Head were significantly larger than those from Oxwich Bay, suggesting site-specific variation in parasite development. The higher prevalence, intensity, and cyst size at Mumbles likely reflect habitat and host-community differences between sites, including greater availability of first-intermediate hosts and differences in crab size cohorts and microhabitats that may influence transmission.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12923448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced YOLO-based framework and benchmarking for automated Plasmodium vivax detection.","authors":"Vivek Morris Prathap, Sonam Yadav","doi":"10.1007/s00436-026-08642-0","DOIUrl":"10.1007/s00436-026-08642-0","url":null,"abstract":"<p><p>Despite remarkable progress in global health and increased life expectancy in the 21st century, malaria remains a major parasitic disease-causing significant morbidity and mortality, particularly in sub-Saharan Africa and Southeast Asia. Traditional diagnostic methods such as rapid diagnostic tests (RDTs) and microscopy, though widely used, suffer from limitations including biomarker mutations, reduced sensitivity at low parasite counts, and operator dependency. These challenges underscore the need for intelligent, automated diagnostic solutions. Artificial intelligence (AI)-driven computer vision methods, especially deep learning (DL)-enabled object detection architectures, offer promising alternatives for automated parasite identification. This study aims to perform a comparative evaluation of YOLO variants (v3, cascade v3, scaled v4, v5, and v8) to determine the most suitable architecture for precise and efficient detection of malaria. The models were assessed with following parameters such as, precision, accuracy, F1-score, recall and mean Average Precision (mAP). This study proposes a novel DL framework that combines YOLOv3 with a modified MobileNetV2 backbone, augmented by a Transformed Convolutional Layer (TCL)exhibits multi-scale texture sensitivity and efficient feature extraction, making it particularly effective for analyzing dense thick smear images of Plasmodium vivax (P. vivax).These findings provide guidance for deploying AI-based malaria diagnostic tools across varied clinical and field settings.This approach aims to contribute toward developing a robust, scalable, and interpretable diagnostic framework for early malaria detection and improved disease management.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":"29"},"PeriodicalIF":2.0,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Toxoplasma gondii prevalence and its impact on tick-borne encephalitis virus infection among humans in Heilongjiang province of China.","authors":"Wanting Li, Lu Zhang, Wei Wang, Weiyan Yin, Feiluran Maimaiti, Mengru Zhao, Xiaohong Peng, Wenzhong Wu, Shouxu Lv, Zhijun Hou","doi":"10.1007/s00436-026-08651-z","DOIUrl":"10.1007/s00436-026-08651-z","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":"30"},"PeriodicalIF":2.0,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146228089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential threat of Strongyloides spp. to mountain gorillas and public health in Bwindi Impenetrable National Park, Uganda.","authors":"Nelson Bukamba, Kelly Marie Sambucci, Eva Nosková, Vojtech Baláž, Ricky Okwir Okello, Dilly Dan Muhumuza, Fred Nizeyimana, Neil Donald Sargison, Rob Francis Kelly, Benard Ssebide, Tierra Smiley Evans, Kirsten Gilardi, Klára Judita Petrželková, Barbora Pafčo, Barbora Červená","doi":"10.1007/s00436-026-08643-z","DOIUrl":"10.1007/s00436-026-08643-z","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13046668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of environmental risk factors, prevalence, and associated symptoms of Giardia intestinalis infection across age groups in Poland: a survey-based study.","authors":"Sylwia Klimczak, Kacper Packi, Alicja Rudek, Marcin Kurowski, Agnieszka Śliwińska","doi":"10.1007/s00436-026-08631-3","DOIUrl":"10.1007/s00436-026-08631-3","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":"28"},"PeriodicalIF":2.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variation and evolutionary relationships of dominant ticks in Northwestern Iran utilizing COI and ITS2.","authors":"Meysam Motevasseli, Eslam Moradi-Asl, Parisa Soltan-Alinejad","doi":"10.1007/s00436-026-08650-0","DOIUrl":"10.1007/s00436-026-08650-0","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":"27"},"PeriodicalIF":2.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12913328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of cestodes from rodents in the Mazury Lake District region of Poland.","authors":"Wiktoria Romanek, Mohammed Alsarraf, Mustafa Alsarraf, Dagmara Wężyk, Dorota Dwużnik-Szarek, Aleksander Goll, Joanna Górska, Martyna Krupińska, Maciej Grzybek, Katarzyna Tołkacz, Jolanta Behnke-Borowczyk, Jerzy M Behnke, Anna Bajer","doi":"10.1007/s00436-026-08629-x","DOIUrl":"10.1007/s00436-026-08629-x","url":null,"abstract":"<p><p>Rodents serve as hosts for many species of parasites, including cestodes, but the taxonomy of rodent-infecting tapeworms, especially in Central Europe, is still understudied. Therefore, the current study was conducted to identify larval and adult cestodes in free-living rodents from Northeastern Poland. The fieldwork was conducted between 2014 and 2023 in three forest sites. Cestodes were collected from five rodent species (Clethrionomys glareolus, Alexandromys oeconomus, Microtus agrestis, Apodemus agrarius and A. flavicollis, and were identified through morphology and by several genetic markers (12S rDNA, cox1, nad 1, 28S rDNA, 12S-16S rDNA) using phylogenetic analyses. A total of 58 cestode specimens were analyzed using molecular and phylogenetic analyses. Species identified included Catenotaenia henttoneni, Spasskijela kratochvili, Mesocestoides litteratus, Mesocestoides melesi, and others. Three Paranoplocephala specimens were identified as P. kalelai based on nad1 sequences. Morphological and genetic analyses indicated that the dominant adult cestode in bank vole was C. henttoneni and Mesocestoides spp. were the dominant larval cestodes. This study combined genetic and morphological methods to identify the cestodes infecting rodents in Poland. It confirmed the existence of P. kalelai in Poland, suggesting that previously reported Paranoplocephala spp., identified by morphological characters, may have corresponded to this molecularly identified species. Our study also highlighted gaps in genetic databases that currently still do not allow precise species identification.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":"26"},"PeriodicalIF":2.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Toxoplasma activity of NBMPR is mediated through the inhibition of nucleoside and nucleobase transporters.","authors":"Ming Pan, Shu-Qin Tang, Ceng-Ceng Ge, Shi-Jie Fan, He-Xin He, Man-Man Bian, Bang Shen, Si-Yang Huang","doi":"10.1007/s00436-026-08646-w","DOIUrl":"10.1007/s00436-026-08646-w","url":null,"abstract":"","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"125 1","pages":"25"},"PeriodicalIF":2.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}