Katarzyna Anna Hołówka, Andrada Negoescu, Marian Taulescu, Angela Monica Ionică, Georgiana Deak, Andrei Daniel Mihalca, Călin Mircea Gherman
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One individual (over three-year-old male found as roadkill in Tureni, Cluj County, Northwestern Romania) had Microtetrameres-like nematodes; a section of its proventriculus was preserved in 10% formalin for histopathological examination. In the remaining positive cases, nematodes were preserved in ethanol for molecular analyses and in 4% formalin for morphological identification. Overall, 25 buzzards were infected with M. cloacitectus, resulting in a prevalence of 28.4% (95% CI 20.0-38.6%). A total of 779 specimens (464♀, 315♂) were recovered, with an intensity range of 1-316 parasites (mean = 31.2). Infections showed a seasonal pattern, peaking in summer, and were more frequent in birds over 2 years old. Molecular analysis yielded a novel cox1 sequence and revealed new phylogenetic relationships. Histopathology revealed multifocal glandular distention and inflammatory nodules centred on parasites. 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引用次数: 0
摘要
cloacitectus是四虫科的一种线虫,寄生于昼行性猛禽的前脑室腺。Oschmarin(1956)首先在俄罗斯滨海边疆区的欧亚秃鹰(Buteo Buteo)中描述了它,此后在几种猛禽物种中也有报道。2017年至2024年间,在罗马尼亚的不同地区收集了88只死去的欧亚秃鹫。记录每个标本的GPS坐标、性别和年龄,并进行完整的寄生虫学尸检。其中一人(在罗马尼亚西北部克卢日县的图雷尼被发现的三岁以上的男性被路杀)有类似小四虫的线虫;其前脑室切片保存在10%福尔马林中进行组织病理学检查。在其余阳性病例中,将线虫保存在乙醇中进行分子分析,并在4%福尔马林中进行形态学鉴定。总共有25只秃鹰感染了阴囊支原体,患病率为28.4% (95% CI 20.0-38.6%)。共检获寄生虫779只(♀464只,♂315只),强度范围1 ~ 316只(平均31.2只)。感染呈季节性,在夏季达到高峰,在2岁以上的禽类中更为常见。分子分析得到了一个新的cox1序列,并揭示了新的系统发育关系。组织病理学显示多灶性腺体扩张和以寄生虫为中心的炎性结节。这项研究提供了罗马尼亚第一个全面的寄生虫学,分子和组织病理学特征。
Microtetrameres cloacitectus in Eurasian buzzard (Buteo buteo): pathology, phylogenetics, and seasonality.
Microtetrameres cloacitectus is a nematode of the family Tetrameridae Travassos, 1914, parasitising the proventricular glands of diurnal raptors. First described by Oschmarin (1956) from the Eurasian buzzard (Buteo buteo) in Primorsky Krai, Russia, it has since been reported in several raptor species. Between 2017 and 2024, 88 deceased Eurasian buzzards were collected from various regions in Romania. For each specimen, GPS coordinates, sex, and age were recorded, and full parasitological necropsies were performed. One individual (over three-year-old male found as roadkill in Tureni, Cluj County, Northwestern Romania) had Microtetrameres-like nematodes; a section of its proventriculus was preserved in 10% formalin for histopathological examination. In the remaining positive cases, nematodes were preserved in ethanol for molecular analyses and in 4% formalin for morphological identification. Overall, 25 buzzards were infected with M. cloacitectus, resulting in a prevalence of 28.4% (95% CI 20.0-38.6%). A total of 779 specimens (464♀, 315♂) were recovered, with an intensity range of 1-316 parasites (mean = 31.2). Infections showed a seasonal pattern, peaking in summer, and were more frequent in birds over 2 years old. Molecular analysis yielded a novel cox1 sequence and revealed new phylogenetic relationships. Histopathology revealed multifocal glandular distention and inflammatory nodules centred on parasites. This study provides the first comprehensive parasitological, molecular, and histopathological characterisation of M. cloacitectus in Romania.
期刊介绍:
The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite.
Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology;
Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.