Pharmaceutical Biology最新文献

{"title":"Anti-fibrosis effect of astragaloside IV in animal models of cardiovascular diseases and its mechanisms: a systematic review.","authors":"Shiyu Zhang, Shijie Li, Xue Li, Chen Wan, Lin Cui, Youping Wang","doi":"10.1080/13880209.2025.2488994","DOIUrl":"https://doi.org/10.1080/13880209.2025.2488994","url":null,"abstract":"<p><strong>Context: </strong>Myocardial fibrosis is a common manifestation of end-stage cardiovascular disease, but there is a lack of means to reverse fibrosis. Astragaloside IV (AS-IV), the major active component of <i>Astragalus membranaceus</i> Fisch. ex Bunge Fabaceae, possesses diverse biological activities that have beneficial effects against cardiovascular disease.</p><p><strong>Objective: </strong>This systematic review aims to summarize the anti-fibrosis effect of AS-IV in animal models (rats or mice only) and its underlying mechanisms, and provide potential directions for the clinical use of AS-IV.</p><p><strong>Methods: </strong>PubMed, EMBASE, Web of Science, CNKI, Wanfang database, and SinoMed were searched from inception to 31 December 2024. The following characteristics of the included studies were extracted and summarized: animal model, route of administration, dose/concentration, measurement indicators, and potential mechanisms. The quality of the included studies was assessed used a 10-item scale from SYRCLE.</p><p><strong>Results and conclusion: </strong>AS-IV represents a promising multi-target candidate for myocardial fibrosis treatment in the 24 eligible studies included in the analysis. This systematic review is the first to comprehensively evaluate the anti-fibrosis mechanisms of AS-IV across heterogeneous cardiovascular disease animal models, including myocardial infarction, hypertension, ischemia-reperfusion injury, and myocarditis. The underlying mechanisms of the anti-fibrosis effects of AS-IV may include collagen metabolism, anti-apoptosis, anti-inflammation and, pyroptosis, antioxidants, improving mitochondrial function, regulating senescence, etc. Current evidence remains preclinical, with critical gaps in toxicological profiles, human safety thresholds, and clinical adverse reaction data. Future research must integrate robust toxicological evaluations, optimized combination therapies, and adaptive clinical trials to validate translational potential.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"250-263"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yiqi Wenyang decoction protects against the development of atherosclerosis by inhibiting vascular inflammation.","authors":"Shuang Hua, Lingling Sun, Han Zhang, Chiwen Shiu, Shujie Zhang, Yao Zhu, Xingqun Yan, Ping Gu, Zhe Huang, Weimin Jiang","doi":"10.1080/13880209.2025.2492650","DOIUrl":"https://doi.org/10.1080/13880209.2025.2492650","url":null,"abstract":"<p><strong>Context: </strong>Vascular inflammation is a key process in the pathogenesis of atherosclerosis, which is regulated by NF-κB pathway. Yiqi Wenyang decoction (YQWY), a Traditional Chinese medicine (TCM) formula, has anti-inflammatory properties and may inhibit this pathway, potentially offering anti-atherosclerotic effects.</p><p><strong>Objective: </strong>The purpose of this study is to investigate the effects of YQWY on atherosclerosis and the underlying mechanism. Materials and methods: ApoE^-/- mice were fed a Western diet and administered with YQWY (low or high dose), atorvastatin, or vehicle for 13 weeks. The size of atherosclerotic plaques was assessed using ORO staining. Vascular inflammation was evaluated with IF or IHC staining. The mechanisms and signaling pathways underlying the effect of YQWY on vasculature were studied using transcriptomic analysis and were validated <i>in vitro</i> in endothelial cells and macrophages.</p><p><strong>Results: </strong>YQWY attenuated atherosclerotic plaque development which was associated with reduced vascular inflammation as demonstrated by transcriptomic analysis of aorta. This was verified by reduced expression of proinflammatory chemokines, adhesion molecules, and inflammatory cytokines in aortas from YQWY-treated mice at both mRNA and protein levels. Mechanistically, YQWY suppressed NF-κB activation in endothelial cells and, to a lesser extent, macrophages possibly.</p><p><strong>Discussion and conclusions: </strong>YQWY protects against vascular inflammation and atherosclerosis by suppressing NF-κB pathway, suggesting the potential of YQWY and its active ingredients as novel anti-atherosclerotic therapeutics.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"264-274"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated pharmacoanalysis, bioinformatics analysis, and experimental validation to identify the ingredients and mechanisms of Xiao-Luo-Wan in uterine fibroids treatment.","authors":"Yonghui Yu, Haojun Zhang, Fang Yang, Hong Liu","doi":"10.1080/13880209.2025.2485905","DOIUrl":"https://doi.org/10.1080/13880209.2025.2485905","url":null,"abstract":"<p><strong>Context: </strong>Xiao-Luo-Wan (XLW), a classical prescription in traditional Chinese medicine, has therapeutic effects on uterine fibroids (UFs). Herein, its anti-UF effects were examined using a systematic pharmacological method.</p><p><strong>Objective: </strong>To explore the active ingredients of XLW <i>via</i> mass spectrometry and its potential effects on UFs by network pharmacology, molecular docking, and experimental validation.</p><p><strong>Materials and methods: </strong>A mass spectrometer was used to scrutinize the composition of the XLW drug-containing serum. The critical targets and potential mechanisms of XLW against UFs were predicted by network pharmacology and molecular docking. Next, human uterine leiomyoma cells (UMCs) were treated with 20%, 30%, or 40% XLW serum for 24 h, 48 h or 72 h. Cell viability was analyzed <i>via</i> a CCK-8 assay, and cell apoptosis and the cell cycle were examined <i>via</i> flow cytometry. The predicted targets were further identified by RT-PCR and western blotting.</p><p><strong>Results: </strong>There were 16 chemical components identified in XLW drug-containing serum, with 53 target genes predicated in the treatment of UFs. The molecular binding of core targets, including TRIM9, NF-κB and p38MAPK, was relatively stable to components, especially buergerinin B, cedrol and ent-15B-16-epoxy- kauan-17-ol. The <i>in vitro</i> experiments revealed that the IC₅₀ of XLW in UMCs was 63.21%, and the anti-UF effects of XLW may be closely associated with targets that inhibit cell proliferation and promote cell apoptosis by regulating TRIM9, NF-κB and p38MAPK expression.</p><p><strong>Discussion and conclusions: </strong>The integration of mass spectrometry, network pharmacology, molecular docking and biological experiments revealed the key constituents of XLW and its pharmacological mechanism in UFs, which may help in the discovery of therapeutic agents for treating UFs.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"201-217"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant activity of flavones from traditional Chinese medicine: a meta-analysis.","authors":"Qing Wang, Youyuan Lu, Xue Mi, Caiyan Yang, Wei Ma, Changbo Xia, Hanqing Wang","doi":"10.1080/13880209.2025.2467374","DOIUrl":"10.1080/13880209.2025.2467374","url":null,"abstract":"<p><strong>Context: </strong>Flavones, the key active components in Traditional Chinese Medicine (TCM), have demonstrated antidepressant activity. Given the numerous animal studies conducted, a systematic analysis is essential to provide a valuable reference for future research.</p><p><strong>Object: </strong>This study investigated the antidepressant activity of flavones based on animal models and summarized the underlying mechanisms.</p><p><strong>Methods: </strong>We systematically searched 7 bibliographic Databases as of August 12, 2023, such as Web of Science, PubMed, China National Knowledge Infrastructure, etc. The meta-analysis was performed using either the random or fixed-effect model, supplemented by trial sequential analysis (TSA). The Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach was used to assess the quality of evidence.</p><p><strong>Results: </strong>A total of 25 studies involving 458 mice were included, identifying five flavones (baicalin, baicalein, apigenin, luteolin, vitexin) with antidepressant activity. Compared to the control group, flavones significantly reduced immobility time in forced swimming and tail suspension tests. Flavones also decreased serum and hippocampal levels of interleukin (IL)-1β and tumor necrosis factor-alpha (TNF-α), reduced nuclear factor kappa B (NF-κB) levels, and increased brain-derived neurotrophic factor (BDNF) levels. Relative to the positive group, flavones raised IL-6, sucrose preference rate, and corticosterone (CORT) levels, with no significant differences in other factors. The TSA showed the efficacy of flavones for treating depression with adequate 'information size' for the primary outcome.</p><p><strong>Conclusions: </strong>The results demonstrate that flavones exert protective effects against depression in mice, primarily by stimulating neurotrophic factors and modulating inflammatory pathways. These findings emphasize their potential as promising candidates for the development of novel antidepressant therapies.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"156-169"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotoxic assessment of a <i>Cannabis sativa</i> L. extract.","authors":"Mark J Tallon, Robert B Child, Jason L Blum","doi":"10.1080/13880209.2025.2499075","DOIUrl":"https://doi.org/10.1080/13880209.2025.2499075","url":null,"abstract":"<p><strong>Context: </strong>As a naturally occurring terpenoid found in <i>Cannabis sativa</i> L. (Cannabaceae), cannabidiol (CBD) has gained public and industry interest for the purposes of personal well-being as a foodstuff and pharmaceutical. Despite a number of publications on CBD toxicology, many have significant limitations, especially those relating to genotoxicity. These include poor characterization of the CBD extract and/or lack rigor in conforming to accepted regulatory guidelines and best practice. A number of regulatory agencies have highlighted these issues and requested additional genotoxicity data to help ensure the safe use of CBD.</p><p><strong>Objective: </strong>To provide insights into the genotoxicity of a CBD isolate and its lipid carrier.</p><p><strong>Materials and methods: </strong>We have conducted an <i>in vitro</i> mammalian cell micronucleus (OECD 487) and a bacterial reverse mutagenicity assay (Ames test) (OECD 471) in a CBD isolate (97% > CBD) with its carrier.</p><p><strong>Results: </strong>The samples tested were non-mutagenic, as determined in the Ames test. The <i>in vitro</i> micronucleus assay conducted was negative for genotoxicity, with no statistically significant increases in the incidences of micronucleated cells observed at any dose compared to negative controls.</p><p><strong>Conclusions: </strong>These studies confirm that this CBD rich isolate in combination with its carrier, are unlikely to post any genotoxic hazard at exposure levels expected in foods.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"357-363"},"PeriodicalIF":3.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of the potential mechanism of gansui in blocking non-small cell lung cancer progression.","authors":"Xiaoxu Yang, Wenlan Li","doi":"10.1080/13880209.2025.2471844","DOIUrl":"10.1080/13880209.2025.2471844","url":null,"abstract":"<p><strong>Context: </strong>Gansui [<i>Euphorbia kansui</i> T. N. Liou ex S.B.Ho (Euphorbiaceae)] has been reported to inhibit the proliferation of non-small cell lung cancer (NSCLC) cells; however, its underlying pharmacological mechanism remains unclear.</p><p><strong>Objective: </strong>To investigate the potential effects and mechanisms of Gansui in blocking NSCLC progression.</p><p><strong>Materials and methods: </strong>The targets of Gansui's components and NSCLC-related targets were obtained through public database and published studies. Functional enrichment analysis was performed using the clusterProfiler R package. STRING database was used for protein-protein interaction analysis. CytoHubba plugin was applied to get the hub genes. Molecular docking was applied to assess the binding affinities between the hub targets and the crucial components. Kidjolanin was used to treat A549 and NCI-H1385, and its effects on cell viability, sensitivity of paclitaxel and expression levels of hub genes were investigated by cell counting kit-8 assay, flow cytometry and qPCR.</p><p><strong>Results: </strong>A total of 16 Gansui active ingredients and 337 targets were collected, of which 298 targets overlapped with NSCLC-related genes. <i>STAT3</i>, <i>EGFR</i>, <i>GRB2</i>, <i>AKT2, AKT3</i> and <i>PIK3CA</i> were identified as hub genes. The components in Gansui, including kidjoranin 3-O-β-digitoxopyranoside, cynotophylloside B, 13-Oxyingenol-dodecanoate, and kidjolanin had good binding affinity with the hub targets. Kidjolanin inhibited the viability of NSCLC cells, promoted apoptosis and inhibited the expression of hub genes. Kidjolanin also enhanced the proliferation inhibition and apoptosis of NSCLC cells induced by paclitaxel.</p><p><strong>Discussion and conclusion: </strong>Gansui exerts anti-NSCLC effects <i>via</i> multiple downstream targets, implying its potential in NSCLC treatment.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"170-187"},"PeriodicalIF":3.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11878171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable cultivation of phytopharmaceuticals in Baden-Wuerttemberg, Germany: a SWOT analysis and future directions.","authors":"Peter W Heger, Ilka Meinert, Peter Nick, Peter Riedl, Michael Heinrich, Michael Straub","doi":"10.1080/13880209.2025.2457328","DOIUrl":"10.1080/13880209.2025.2457328","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this systematic work is a Strengths, Weaknesses, Opportunities, Threats (SWOT) analysis to select suitable medicinal plants for cultivation in the region of Baden-Wuerttemberg, Germany.</p><p><strong>Methods: </strong>A systematic SWOT analysis, based on expert assesments and literature research, was performed considering factors like market demand, cultivation conditions, and potential economic benefits.</p><p><strong>Results: </strong>Medicinal plants have been essential for producing compounds with significant health benefits. However, unsuitable harvesting practices threaten plant species and traditional communities due to loss of knowledge and culture. In response, sustainable cultivation is gaining attention as alternative to wild collection, ensuring both biodiversity conversation and integrity of medicinal products. Three plants - <i>Arnica montana L.</i>, <i>Hydrastis canadensis L.,</i> and <i>Rheum rhaponticum L.</i> - were identified as particularly suitable due to their high demand and feasibility of their cultivation under local conditions. Conversely, six other plants were deemed less viable due to various challenges, including market competition and harvesting difficulties.</p><p><strong>Conclusions: </strong>This publication emphasizes the importance of comprehensive planning and analysis in transitioning from wild collection to sustainable cultivation of medicinal plants, highlighting the potential benefits for regional agriculture, conservation efforts, and the pharmaceutical industry. BIOPRO Baden-Württemberg GmbH promotes this approach by fostering a bioeconomy centred on cultivating high-value medicinal plants in the state of Baden-Wuerttemberg, Germany.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"82-88"},"PeriodicalIF":3.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential mechanism of Qinggong Shoutao pill alleviating age-associated memory decline based on integration strategy.","authors":"Guiyun Pan, Lijuan Chai, Rui Chen, Qing Yuan, Zhihui Song, Wanying Feng, Jinna Wei, Zhihua Yang, Yuhang Zhang, Guinan Xie, An Yan, Qingbo Lv, Caijun Wang, Yingqiang Zhao, Yi Wang","doi":"10.1080/13880209.2023.2291689","DOIUrl":"10.1080/13880209.2023.2291689","url":null,"abstract":"<p><strong>Context: </strong>Qinggong Shoutao Wan (QGSTW) is a pill used as a traditional medicine to treat age-associated memory decline (AAMI). However, its potential mechanisms are unclear.</p><p><strong>Objective: </strong>This study elucidates the possible mechanisms of QGSTW in treating AAMI.</p><p><strong>Materials and methods: </strong>Network pharmacology and molecular docking approaches were utilized to identify the potential pathway by which QGSTW alleviates AAMI. C57BL/6J mice were divided randomly into control, model, and QGSTW groups. A mouse model of AAMI was established by d-galactose, and the pathways that QGSTW acts on to ameliorate AAMI were determined by ELISA, immunofluorescence staining and Western blotting after treatment with d-gal (100 mg/kg) and QGSTW (20 mL/kg) for 12 weeks.</p><p><strong>Results: </strong>Network pharmacology demonstrated that the targets of the active components were significantly enriched in the cAMP signaling pathway. AKT1, FOS, GRIN2B, and GRIN1 were the core target proteins. QGSTW treatment increased the discrimination index from -16.92 ± 7.06 to 23.88 ± 15.94% in the novel location test and from -19.54 ± 5.71 to 17.55 ± 6.73% in the novel object recognition test. ELISA showed that QGSTW could increase the levels of cAMP. Western blot analysis revealed that QGSTW could upregulate the expression of PKA, CREB, c-Fos, GluN1, GluA1, CaMKII-α, and SYN. Immunostaining revealed that the expression of SYN was decreased in the CA1 and DG.</p><p><strong>Discussion and conclusions: </strong>This study not only provides new insights into the mechanism of QGSTW in the treatment of AAMI but also provides important information and new research ideas for the discovery of traditional Chinese medicine compounds that can treat AAMI.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"62 1","pages":"105-119"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10763866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cephaeline promotes ferroptosis by targeting NRF2 to exert anti-lung cancer efficacy.","authors":"Peng Chen, Qingxuan Ye, Shang Liang, Linghui Zeng","doi":"10.1080/13880209.2024.2309891","DOIUrl":"10.1080/13880209.2024.2309891","url":null,"abstract":"<p><strong>Context: </strong>Cephaeline is a natural product isolated from ipecac (<i>Cephaelis ipecacuanha</i> [Brot.] A. Rich. [Rubiaceae]). It exhibits promising anti-lung cancer activity and ferroptosis induction may be a key mechanism for its anti-lung cancer effect.</p><p><strong>Objectives: </strong>This study investigates the anti-lung cancer activity and mechanisms of cephaeline both <i>in vitro</i> and <i>in vivo</i>.</p><p><strong>Materials and methods: </strong>H460 and A549 lung cancer cells were used. The cephaeline inhibition rate on lung cancer cells was detected <i>via</i> a Cell Counting Kit-8 assay after treatment with cephaeline for 24 h. Subsequently, the concentrations of 25, 50 and 100 nM were used for <i>in vitro</i> experiments. In addition, the antitumour effects of cephaeline (5, 10 mg/kg) <i>in vivo</i> were evaluated after 12 d of cephaeline treatment.</p><p><strong>Results: </strong>Cephaeline showed significant inhibitory effects on lung cancer cells, and the IC₅₀ of cephaeline on H460 and A549 at 24, 48 and 72 h were 88, 58 and 35 nM, respectively, for H460 cells and 89, 65 and 43 nM, respectively, for A549 cells. Meanwhile, we demonstrated that ferroptosis is the key mechanism of cephaeline against lung cancer. Finally, we found that cephaeline induced ferroptosis in lung cancer cells by targeting NRF2.</p><p><strong>Discussion and conclusion: </strong>We demonstrated for the first time that cephaeline inhibits NRF2, leading to ferroptosis in lung cancer cells. These findings may contribute to the development of innovative therapeutics for lung cancer.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"62 1","pages":"195-206"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10860416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism.","authors":"Kun Li, Xue Zheng, Jian Zhang, Zhanpeng Yan, Yu Ji, Fei Ge, Fangshi Zhu","doi":"10.1080/13880209.2024.2309864","DOIUrl":"10.1080/13880209.2024.2309864","url":null,"abstract":"<p><strong>Context: </strong>Jian Gan powder (JGP) is a Chinese medicine compound comprised ginseng, Radix Paeoniae Alba, Radix Astragali, Salvia miltiorrhiza, Yujin, Rhizoma Cyperi, Fructus aurantii, Sophora flavescens, Yinchen, Bupleurum and licorice.</p><p><strong>Objective: </strong>This study explored the inhibitory effects, polarization and potential mechanisms associated with JGP in macrophages.</p><p><strong>Materials and methods: </strong>RAW264.7 cells were randomly divided into six groups for 24 h: control, lipopolysaccharide (LPS), overexpression, 1% JGP, 2% JGP, 4% JGP, 8% JGP and 16% JGP. The effects of JGP on RAW264.7 cell proliferation were assessed using colony formation assays and cell counting kit-8 (CCK-8) assays. The Transwell assay was used to evaluate its impact on RAW264.7 cell migration. Moreover, we analysed the interleukin-6 (IL-6)/signal transducer and activator of the transcription 3 (IL-6/STAT3) signaling pathway using quantitative real-time PCR and Western blotting. Furthermore, we examined the M1/M2 polarization levels.</p><p><strong>Results: </strong>Unlike LPS stimulation, JGP serum treatment markedly suppressed macrophage proliferation and migration capacity, while STAT3 overexpression enhanced RAW264.7 cell proliferation and migration. JGP inhibited the proliferation and migration of RAW264.7 cells by attenuating the IL-6/STAT3 signaling pathway. Furthermore, it inhibited macrophage M1 polarization, promoting M2 polarization.</p><p><strong>Discussion and conclusions: </strong>JGP effectively suppressed the cellular function of RAW264.7 cells by down-regulating the IL-6/STAT3 signaling pathway and modulating macrophage M1/M2 polarization. These findings provide valuable theoretical and experimental basis for considering the potential clinical application of JGP in the treatment of immune-mediated liver injury in clinical practice.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"62 1","pages":"162-169"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}