Pharmaceutical Biology最新文献

{"title":"Direct and indirect targets of carboxyatractyloside, including overlooked toxicity toward nucleoside diphosphate kinase (NDPK) and mitochondrial H⁺ leak.","authors":"Andrzej M Woyda-Ploszczyca","doi":"10.1080/13880209.2023.2168704","DOIUrl":"10.1080/13880209.2023.2168704","url":null,"abstract":"<p><strong>Context: </strong>The toxicity of atractyloside/carboxyatractyloside is generally well recognized and commonly ascribed to the inhibition of mitochondrial ADP/ATP carriers, which are pivotal for oxidative phosphorylation. However, these glycosides may 'paralyze' additional target proteins.</p><p><strong>Objective: </strong>This review presents many facts about atractyloside/carboxyatractyloside and their plant producers, such as <i>Xanthium</i> spp. (Asteraceae), named cockleburs.</p><p><strong>Methods: </strong>Published studies and other information were obtained from databases, such as 'CABI - Invasive Species Compendium', 'PubMed', and 'The World Checklist of Vascular Plants', from 1957 to December 2022. The following major keywords were used: 'carboxyatractyloside', 'cockleburs', 'hepatotoxicity', 'mitochondria', 'nephrotoxicity', and '<i>Xanthium</i>'.</p><p><strong>Results: </strong>In the third decade of the twenty first century, public awareness of the severe toxicity of cockleburs is still limited. Such toxicity is often only perceived by specialists in Europe and other continents. Interestingly, cocklebur is among the most widely distributed invasive plants worldwide, and the recognition of new European stands of <i>Xanthium</i> spp. is provided here. The findings arising from field and laboratory research conducted by the author revealed that (i) some livestock populations may instinctively avoid eating cocklebur while grazing, (ii) carboxyatractyloside inhibits ADP/GDP metabolism, and (iii) the direct/indirect target proteins of carboxyatractyloside are ambiguous.</p><p><strong>Conclusions: </strong>Many aspects of the <i>Xanthium</i> genus still require substantial investigation/revision in the future, such as the unification of the Latin nomenclature of currently distinguished species, bur morphology status, true fruit (achene) description and biogeography of cockleburs, and a detailed description of the physiological roles of atractyloside/carboxyatractyloside and the toxicity of these glycosides, mainly toward mammals. Therefore, a more careful interpretation of atractyloside/carboxyatractyloside data, including laboratory tests using <i>Xanthium</i>-derived extracts and purified toxins, is needed.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"61 1","pages":"372-390"},"PeriodicalIF":3.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10764336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics reveals the effects of hydroxysafflor yellow A on neurogenesis and axon regeneration after experimental traumatic brain injury.","authors":"En Hu, Teng Li, Zhilin Li, Hong Su, Qiuju Yan, Lei Wang, Haigang Li, Wei Zhang, Tao Tang, Yang Wang","doi":"10.1080/13880209.2023.2229379","DOIUrl":"10.1080/13880209.2023.2229379","url":null,"abstract":"<p><strong>Context: </strong>Hydroxysafflor yellow A (HSYA) is the main bioactive ingredient of safflower (<i>Carthamus tinctorius</i> L., [Asteraceae]) for traumatic brain injury (TBI) treatment.</p><p><strong>Objective: </strong>To explore the therapeutic effects and underlying mechanisms of HSYA on post-TBI neurogenesis and axon regeneration.</p><p><strong>Materials and methods: </strong>Male Sprague-Dawley rats were randomly assigned into Sham, controlled cortex impact (CCI), and HSYA groups. Firstly, the modified Neurologic Severity Score (mNSS), foot fault test, hematoxylin-eosin staining, Nissl's staining, and immunofluorescence of Tau1 and doublecortin (DCX) were used to evaluate the effects of HSYA on TBI at the 14th day. Next, the effectors of HSYA on post-TBI neurogenesis and axon regeneration were screened out by pathology-specialized network pharmacology and untargeted metabolomics. Then, the core effectors were validated by immunofluorescence.</p><p><strong>Results: </strong>HSYA alleviated mNSS, foot fault rate, inflammatory cell infiltration, and Nissl's body loss. Moreover, HSYA increased not only hippocampal DCX but also cortical Tau1 and DCX following TBI. Metabolomics demonstrated that HSYA significantly regulated hippocampal and cortical metabolites enriched in 'arginine metabolism' and 'phenylalanine, tyrosine and tryptophan metabolism' including l-phenylalanine, ornithine, l-(+)-citrulline and argininosuccinic acid. Network pharmacology suggested that neurotrophic factor (BDNF) and signal transducer and activator of transcription 3 (STAT3) were the core nodes in the HSYA-TBI-neurogenesis and axon regeneration network. In addition, BDNF and growth-associated protein 43 (GAP43) were significantly elevated following HSYA treatment in the cortex and hippocampus.</p><p><strong>Discussion and conclusions: </strong>HSYA may promote TBI recovery by facilitating neurogenesis and axon regeneration through regulating cortical and hippocampal metabolism, BDNF and STAT3/GAP43 axis.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"61 1","pages":"1054-1064"},"PeriodicalIF":3.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10332220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of berberine for premature ventricular contractions: a meta-analysis and systematic review of randomized controlled trials.","authors":"Meng Qiao,&nbsp;Chao Lei,&nbsp;Chaoren Tan,&nbsp;Cuncun Lu,&nbsp;Zijia Chen,&nbsp;Qiang Zhang,&nbsp;Zhifei Wang","doi":"10.1080/13880209.2023.2248167","DOIUrl":"10.1080/13880209.2023.2248167","url":null,"abstract":"<p><strong>Context: </strong>Berberine is a potential drug that can effectively treat cardiovascular diseases, including premature ventricular contractions (PVCs).</p><p><strong>Objective: </strong>This study was conducted to assess the efficacy and safety of berberine for PVCs.</p><p><strong>Methods: </strong>The literature was searched using PubMed, Cochrane Library, Embase, China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP), Wanfang, and Chinese Biomedical Literature Database (CBM) for randomized controlled trials (RCTs) from inception to October 1, 2022. The risk of bias was assessed using the Revised Cochrane risk-of-bias tool for randomized trials, and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system was adopted to assess the quality of evidence.</p><p><strong>Results: </strong>Ten RCTs with 896 participants were included in the meta-analysis. The results showed that compared to antiarrhythmic drugs (AD), berberine (BE) combined with AD had a higher effective rate (RR = 1.26; 95% CI:1.12, 1.42; <i>p</i> = 0.0001) with no significant incidence of adverse reactions (RR = 0.93; 95% CI:0.33, 2.57; <i>p</i> = 0.88), and BE alone had no significant difference in effective rate (RR = 0.91; 95% CI:0.77, 1.07; <i>p</i> = 0.23), and a lower incidence of adverse reactions (RR = 0.38; 95% CI:0.15, 0.97; <i>p</i> = 0.04) and recurrence rate (RR = 0.40; 95% CI:0.18, 0.88; <i>p</i> = 0.02).</p><p><strong>Conclusions: </strong>The results suggest that BE is an effective and safe adjunctive method for PVCs. In addition, BE is recommended for patients with PVCs who had severe adverse reactions after administrating AD as an alternative therapy.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"61 1","pages":"1474-1483"},"PeriodicalIF":3.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49680938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profile of the Anatolian <i>Sideritis</i> species with bioactivity studies.","authors":"Sema Çarıkçı, Turgut Kılıç, Ahmet C Gören, Tuncay Dirmenci, Gülbahar Özge Alim Toraman, Gülaçtı Topçu","doi":"10.1080/13880209.2023.2280253","DOIUrl":"10.1080/13880209.2023.2280253","url":null,"abstract":"<p><strong>Context: </strong>The genus <i>Sideritis</i> L. (Lamiaceae) is represented by 46 species in Turkey with an 79% endemism ratio, 42 of 46 belonging to the section Empodoclia.</p><p><strong>Objective: </strong>In this review article, <i>Sideritis</i> species growing in Turkey have been evaluated for phytochemical constituents and biological activities.</p><p><strong>Methods: </strong>The data for the isolates, components and extracts of the Anatolian <i>Sideritis</i> species and their bioactivity studies were retrieved from the main databases WoS, Scopus and PubMed from 1975 until 31 December 2022.</p><p><strong>Results: </strong>In this review article, terpenoids, flavonoids, phenolics and other secondary metabolites isolated from Turkish <i>Sideritis</i> species were reported. Anatolian <i>Sideritis</i> species, which primarily consist of monoterpenes and sesquiterpenes, were studied in detail. <i>Sideritis</i> plants are represented by 46 species in Turkey, and 25 of them were investigated for their diterpenoids through isolation or LC-MS studies. Most of the diterpenoids of Turkish <i>Sideritis</i> species have <i>ent</i>-kaurene skeleton, among them linearol, siderol, 7-<i>epi</i>candicandiol and sideridiol were found to be the main compounds. Exceptionally, labdane, pimarane and beyerene diterpenoids were only found in a few species. For phenolics and flavonoids, only 12 species were investigated until now, and they were found to be rich in phenylethanoid glycosides and flavonoid glycosides. In terms of activity, most of the species were tested for antioxidant activity, followed by antimicrobial and anti-ulcer/anti-inflammatory activities. Their cytotoxic, enzyme inhibitory, antinociceptive and antistress activities were less frequently studied.</p><p><strong>Conclusions: </strong><i>Sideritis</i> species should be considered promising therapeutic agents in the treatment of upper respiratory tract and ulcer/inflammatory diseases.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"61 1","pages":"1484-1511"},"PeriodicalIF":3.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11001281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracts of <i>Thesium chinense</i> inhibit SARS-CoV-2 and inflammation <i>in vitro</i>.","authors":"Juncheng Ma,&nbsp;Juanru Wei,&nbsp;Gang Chen,&nbsp;Xiaowei Yan,&nbsp;Hechun Sun,&nbsp;Ning Li","doi":"10.1080/13880209.2023.2253841","DOIUrl":"https://doi.org/10.1080/13880209.2023.2253841","url":null,"abstract":"<p><strong>Context: </strong>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still spreading rapidly. Relevant research based on the antiviral effects of <i>Thesium chinense</i> Turcz (Santalaceae) was not found.</p><p><strong>Objective: </strong>To investigate the antiviral and anti-inflammatory effects of extracts of <i>T. chinense</i>.</p><p><strong>Materials and methods: </strong>To investigate the anti-entry and replication effect of the ethanol extract of <i>T. chinense</i> (drug concentration 80, 160, 320, 640, 960 μg/mL) against the SARS-CoV-2. Remdesivir (20.74 μM) was used as positive control, and Vero cells were used as host cells to detect the expression level of nucleocapsid protein (NP) in the virus by real-time quantitative polymerase chain reaction (RT-PCR) and Western blotting. RAW264.7 cells were used as an anti-inflammatory experimental model under lipopolysaccharide (LPS) induction, and the expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>The ethanol extract of <i>T. chinense</i> significantly inhibited the replication (half maximal effective concentration, EC₅₀: 259.3 μg/mL) and entry (EC₅₀: 359.1 μg/mL) of SARS-CoV-2 into Vero cells, and significantly reduced the levels of IL-6 and TNF-α produced by LPS-stimulated RAW264.7 cells. Petroleum ether (EC₅₀: 163.6 μg/mL), ethyl acetate (EC₅₀: 22.92 μg/mL) and <i>n</i>-butanol (EC₅₀: 56.8 μg/mL) extracts showed weak inhibition of SARS-CoV-2 replication in Vero cells, and reduced the levels of IL-6 and TNF-α produced by LPS-stimulated RAW264.7 cells.</p><p><strong>Conclusion: </strong><i>T. chinense</i> can be a potential candidate to fight SARS-CoV-2, and is becoming a traditional Chinese medicine candidate for treating COVID-19.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"61 1","pages":"1446-1453"},"PeriodicalIF":3.8,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10195017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Er Miao San on peritoneal macrophage polarisation through the miRNA-33/NLRP3 signalling pathway in a rat model of adjuvant arthritis.","authors":"Min Liu,&nbsp;Xiangwen Meng,&nbsp;Zihua Xuan,&nbsp;Simeng Chen,&nbsp;Jin Wang,&nbsp;Zhiluo Chen,&nbsp;Jiayu Wang,&nbsp;Xiaoyi Jia","doi":"10.1080/13880209.2022.2066700","DOIUrl":"https://doi.org/10.1080/13880209.2022.2066700","url":null,"abstract":"<p><strong>Context: </strong>Er Miao San (EMS) is a formulation that contains <i>Atractylodis Rhizoma</i> and <i>Phellodendri Cortex</i> in 1:1 ratio, and is commonly used to treat rheumatoid arthritis (RA) and other inflammatory diseases.</p><p><strong>Objective: </strong>We investigated the mechanism of action and effects of EMS on peritoneal macrophage differentiation in a rat model of adjuvant arthritis (AA).</p><p><strong>Materials and methods: </strong>EMS (3, 1.5 and 0.75 g/kg; once daily) and methotrexate (0.5 mg/kg; once every 3 days) were administered orally from days 21 to 35 after immunisation. Paw swelling and arthritis index were measured; pathological changes in the ankle joint were observed using x-ray and haematoxylin eosin staining. The ratio of CD86/CD206 in macrophages was detected by flow cytometry. Examination of the miRNA-33/NLRP3 signalling pathway was examined by RT-qPCR and western blotting. The levels of cytokines in the serum and cell supernatants were tested by ELISA.</p><p><strong>Results: </strong>EMS significantly reduced the AA index in rats (from 11.0 to 9.3) and pathological changes in the ankle joint (from 3.8 to 1.4). The ratio of CD86/CD206 was reduced, and polarisation to M1 improved (from 0.9 to 0.6) in macrophages of EMS-treated rats. EMS downregulated the miRNA-33/NLRP3 pathway. Furthermore, EMS treatment increased IL-10 and TGF-β levels in the serum and supernatant of macrophages of AA rats and simultaneously decreased the levels of IL-1β and TNF-α.</p><p><strong>Discussion and conclusions: </strong>Our results suggest that EMS may reduce macrophage polarisation to the M1 inflammatory phenotype by downregulating the miRNA-33/NLRP3 pathway in AA rats. These findings may provide new insights into the treatment of RA.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"60 1","pages":"846-853"},"PeriodicalIF":3.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10510800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of lupenone for treating type 2 diabetes by integrating pharmacological evaluation and network pharmacology.","authors":"Feng Xu,&nbsp;Mei Zhang,&nbsp;Hongmei Wu,&nbsp;Yuanmin Wang,&nbsp;Ye Yang,&nbsp;Xiangpei Wang","doi":"10.1080/13880209.2022.2067568","DOIUrl":"https://doi.org/10.1080/13880209.2022.2067568","url":null,"abstract":"<p><strong>Context: </strong>Lupenone (LUP) is the active ingredient of <i>Musa basjoo</i> Sieb. et Zucc. (Musaceae) with antidiabetes effects, but an unclear underlying mechanism of action.</p><p><strong>Objective: </strong>Animal experiments combined with network pharmacology were used to explore the mechanism of LUP for treating diabetes.</p><p><strong>Materials and methods: </strong>Insulin resistance (IR) in male Sprague-Dawley rats with type 2 diabetic was induced using a high-fat diet and streptozotocin. The selected rats were divided into normal group, model group, positive group and LUP (2.0, 4.0 and 8.0 mg/kg) groups, and orally administrated twice daily with Tween 80, rosiglitazone or LUP. Fasting blood glucose (FBG), oxidative stress index, blood lipids and IR-related targets were detected. A network pharmacology analysis was performed.</p><p><strong>Results: </strong>Compared to the model group, LUP (8.0 mg/kg) significantly decreased the levels of FBG (22.3%), LEP (9.5%), HbA1c (14.9%) and MDA (12.3%), increased the ADPN (24.2%) levels and GSH-PX activity (12.4%) (<i>p</i> < 0.05), improved oxidative stress, lipid metabolism disorders and pancreas pathological changes, increased the mRNA and protein expression of InsR (3.7-fold and 1.3-fold), IRS-1 (3-fold and 2-fold), IRS-2 (2-fold and 1.6-fold), GLUT-4 (2-fold and 2.4-fold) in skeletal muscle and IRS-1 (6-fold and 1.6-fold), IRS-2 (5.8-fold and 1.5-fold), GLUT-4 (2.5-fold and 1.7-fold) and PPAR-γ (7-fold and 1.4-fold) in adipose tissue (<i>p</i> < 0.05). Network pharmacology analysis revealed that LUP improves IR by multiple targets and signal pathways.</p><p><strong>Conclusions: </strong>The mechanism of LUP for treating diabetes is related to improving IR. LUP has the potential to be developed as a new drug for treating type 2 diabetes.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"60 1","pages":"997-1010"},"PeriodicalIF":3.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10509071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rb1 reduces oxidative/carbonyl stress damage and ameliorates inflammation in the lung of streptozotocin-induced diabetic rats.","authors":"Hao Su,&nbsp;Cheng-Ju Tian,&nbsp;Ying Wang,&nbsp;Jiaojiao Shi,&nbsp;Xiaoxiao Chen,&nbsp;Zhong Zhen,&nbsp;Yu Bai,&nbsp;Lan Deng,&nbsp;Chunpeng Feng,&nbsp;Zhuang Ma,&nbsp;Jinfeng Liu","doi":"10.1080/13880209.2022.2140168","DOIUrl":"https://doi.org/10.1080/13880209.2022.2140168","url":null,"abstract":"<p><strong>Context: </strong>Ginsenoside Rb1 (Rb1) is a biologically active component of ginseng [<i>Panax ginseng</i> C.A. Meyer (Araliaceae)].</p><p><strong>Objective: </strong>This study determined the underlying mechanisms of Rb1 treatment that acted on diabetes-injured lungs in diabetic rats.</p><p><strong>Materials and methods: </strong>Streptozotocin (STZ)-induced diabetic rat model was used. Male Sprague-Dawley (SD) rats were divided into four groups (<i>n</i> = 10): control, Rb1 (20 mg/kg), insulin (15 U/kg to attain the euglycaemic state) and diabetic (untreated). After treatment for six weeks, oxidative stress assay; histological and ultrastructure analyses; TNF-α, TGF-β, IL-1 and IL-6 protein expression analyses; and the detection of apoptosis were performed.</p><p><strong>Results: </strong>There was decreased activity of SOD (3.53-fold), CAT (2.55-fold) and GSH (1.63-fold) and increased levels of NO (4.47-fold) and MDA (3.86-fold) in the diabetic group from control. Rb1 treatment increased SOD (2.4-fold), CAT (1.9-fold) and GSH (1.29-fold) and decreased the levels of NO (1.76-fold) and MDA (1.51-fold) as compared with diabetic rats. The expression of IL-6 (5.13-fold), IL-1α (2.35-fold), TNF-α (2.35-fold) and TGF-β (2.39-fold) was increased in diabetic rats from control. IL-6 (2.43-fold), IL-1α (2.27-fold), TNF-α (1.68-fold) and TGF-β (2.3-fold) were decreased in the Rb1 treatment group. Diabetes increased the apoptosis rate (2.23-fold vs. control), and Rb1 treatment decreased the apoptosis rate (1.73-fold vs. the diabetic rats). Rb1 and insulin ameliorated lung tissue injury.</p><p><strong>Discussion and conclusions: </strong>These findings indicate that Rb1 could be useful for mitigating oxidative damage and inflammatory infiltration in the diabetic lung.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"60 1","pages":"2229-2236"},"PeriodicalIF":3.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10512391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpinetin suppresses CYP3A4, 2C9, and 2E1 activity <i>in vitro</i>.","authors":"Hongming Song,&nbsp;Chuankui Wei,&nbsp;Wu Yang,&nbsp;Zhaohe Niu,&nbsp;Mingkai Gong,&nbsp;Haiyan Hu,&nbsp;Haibo Wang","doi":"10.1080/13880209.2022.2071450","DOIUrl":"https://doi.org/10.1080/13880209.2022.2071450","url":null,"abstract":"<p><strong>Context: </strong>Alpinetin, the major active constitutes of <i>Alpinia katsumata</i> Hayata (Zingiberaceae), has been demonstrated to possess the activity of anti-breast cancer. Cytochrome P450 enzymes (CYP450s) plays vital roles in the biotransformation of various drugs.</p><p><strong>Objective: </strong>To assess the effect of alpinetin on the activity of CYP450s and estimate the inhibition characteristics.</p><p><strong>Materials and methods: </strong>The activity of CYP450s was evaluated in pooled human liver microsomes with corresponding substrates and marker reactions. The effect of alpinetin was compared with blank control (negative control) and corresponding inhibitors (positive control). The dose-dependent and time-dependent experiments were conducted in the presence of 0, 2.5, 5, 10, 25, 50, and 100 μM alpinetin and incubated for 0, 5, 10, 15, and 30 min.</p><p><strong>Results: </strong>Alpinetin suppressed CYP3A4, 2C9, and 2E1 activity. All the inhibitions were significantly influenced by alpinetin contration with the IC₅₀ values of 8.23 μM (CYP3A4), 12.64 μM (CYP2C9), and 10.97 μM (CYP2E1), respectively. The inhibition of CYP3A4 was fitted with the non-competitive model with a <i>Ki</i> value of 4.09 μM and was time-dependent with <i>KI</i> and <i>Kinact</i> values of 4.67 min and 0.041 μM^-1, respectively. While CYP2C9 and 2E1 were inhibited by alpinetin competitively with <i>Ki</i> values of 6.42 (CYP2C9) and 5.40 μM (CYP2E1), respectively, in a time-independent manner.</p><p><strong>Discussion and conclusion: </strong>The <i>in vitro</i> inhibitory effect of alpineticn on CYP3A, 2C9, and 2E1 implied the potential interaction of alpinetin or its origin herbs with the drugs metabolised by those CYP450s, which needs further <i>in vivo</i> validation.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"60 1","pages":"1032-1037"},"PeriodicalIF":3.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10515495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics.","authors":"Shan-Peng Ma,&nbsp;Wei-Ping Ma,&nbsp;Shi-Ning Yin,&nbsp;Xiang-Yue Chen,&nbsp;Xiao-Qing Ma,&nbsp;Bao-Hong Wei,&nbsp;Jing-Guang Lu,&nbsp;Hong-Bing Liu","doi":"10.1080/13880209.2022.2074465","DOIUrl":"https://doi.org/10.1080/13880209.2022.2074465","url":null,"abstract":"<p><strong>Context: </strong>Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear.</p><p><strong>Objective: </strong>To investigate the antiperspirant effect and underlying mechanisms of MLS.</p><p><strong>Materials and methods: </strong>Fifty rats were divided into control, model, and three doses of MLS intervention groups (<i>n</i> = 10). Rats except for control group were induced diseases features of the applicable scope of MLS <i>via</i> i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats <i>via</i> s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis <i>via</i> UPLC-QE-Focus-MS.</p><p><strong>Results: </strong>Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio <i>via</i> increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group (<i>p</i> < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS (<i>p</i> < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism.</p><p><strong>Conclusions: </strong>MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"60 1","pages":"1055-1062"},"PeriodicalIF":3.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10515496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}