Hydroethanolic extracts from Bauhinia guianensis: a study on acute toxicity in Zebrafish embryos and adults.

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Pharmaceutical Biology Pub Date : 2024-12-01 Epub Date: 2024-07-17 DOI:10.1080/13880209.2024.2374806
Rosemary de Carvalho Rocha Koga, Gisele Custodio de Souza, Abrahão Victor Tavares de Lima Teixeira, Adriana Maciel Ferreira, Brenda Lorena Sánchez-Ortiz, Lucas Silva Abreu, Josean Fechine Tavares, José Carlos Tavares Carvalho
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引用次数: 0

Abstract

Context: The botanical species Bauhinia guianensis Aublet (Leguminosae-Cercidoideae) is traditionally used in the Amazon for medicinal purposes.

Objective: The acute toxicity of the hydroethanolic extracts from B. guianensis leaves and stems (HELBg and HESBg) was evaluated in zebrafish (Danio rerio), with emphasis on the embryonic developmental stage and adult alterations.

Materials and methods: Extracts were analyzed on LC-DAD-MS/MS. Zebrafish eggs were inoculated individually with concentrations of HELBg and HESBg (0.25, 0.5, 0.75, 1.0, and 1.5 µg/mL), observed for 96 h. Adult zebrafish were treated with a single oral dose (100, 200, 500, 1000, and 2000 mg/kg) of HELBg and HESBg, observed for 48 h.

Results: HELBg and HESBg analysis detected 55 compounds. Both extracts exhibited toxicity, including embryo coagulation at higher doses of HELBg and absence of heartbeats in embryos at all doses of HESBg. Behavioral variations were observed; tissue alterations in adult zebrafish were found at the highest doses, primarily in the liver, intestine, and kidneys because of HELBg and HESBg effects. The LD50 of HESBg was 1717 mg/kg, while HELBg exceeded the limit dose of 2000 mg/kg.

Conclusions: The study on acute toxicity of B. guianensis extracts exhibits significant toxic potential, emphasizing effects on embryonic and adult zebrafish. The results suggest relative safety of the species preparations, encouraging further clinical trials on potential biological activities.

洋紫荆的水乙醇提取物:斑马鱼胚胎和成鱼急性毒性研究。
背景:植物物种洋紫荆(Bauhinia guianensis Aublet)(豆科-芹菜属)是亚马逊地区的传统药用植物:目的:评估了洋紫荆叶和茎的水乙醇提取物(HELBg 和 HESBg)对斑马鱼(Danio rerio)的急性毒性,重点是胚胎发育阶段和成年斑马鱼的变化:提取物通过 LC-DAD-MS/MS 进行分析。分别给斑马鱼卵接种浓度为 0.25、0.5、0.75、1.0 和 1.5 µg/mL 的 HELBg 和 HESBg,观察 96 小时;给成年斑马鱼单次口服剂量(100、200、500、1000 和 2000 mg/kg)的 HELBg 和 HESBg,观察 48 小时:结果:HELBg 和 HESBg 分析检测出 55 种化合物。两种提取物都表现出毒性,包括较高剂量的 HELBg 会导致胚胎凝结,所有剂量的 HESBg 都会导致胚胎无心跳。由于 HELBg 和 HESBg 的影响,观察到了行为上的变化;在最高剂量下,成年斑马鱼的组织发生了改变,主要是肝脏、肠道和肾脏。HESBg 的半数致死剂量为 1717 毫克/千克,而 HELBg 则超过了 2000 毫克/千克的极限剂量:结论:B. guianensis 提取物的急性毒性研究显示了其显著的毒性潜力,尤其是对胚胎和成年斑马鱼的影响。研究结果表明该物种制剂相对安全,鼓励对其潜在的生物活性进行进一步的临床试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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