Journal of Pineal Research最新文献

{"title":"Melatonin-Mediated Modulation of Polyamines Enhances Drought Tolerance in Foxtail Millet (Setaria italica L.)","authors":"Qifeng Shi,&nbsp;Guo Wang,&nbsp;Tao Zhang,&nbsp;Yijia Zhao,&nbsp;Fanyi Meng,&nbsp;Ke Feng,&nbsp;Xiaoxiao Yang,&nbsp;Juan Zhao,&nbsp;Xiangyang Yuan,&nbsp;Jianhong Ren","doi":"10.1111/jpi.70157","DOIUrl":"10.1111/jpi.70157","url":null,"abstract":"<div>\u0000 \u0000 <p>Melatonin have been proposed as a potential alternative in enhancing drought tolerance. However, the mechanisms underlying remain poorly understood. This study tested the hypothesis that melatonin improves drought tolerance by regulating polyamine metabolism. Hydroponically grown foxtail millet (<i>Setaria italica</i> L.) seedlings were subjected to drought stress induced by polyethylene glycol (PEG-6000, 10%), with or without melatonin application. The results showed that exogenous melatonin improved drought tolerance by improving water uptake, increasing root hydraulic conductivity (L_pr) and whole-plant hydraulic conductance (K_plant) by 37% and 33%, respectively. Melatonin also significantly increased the activities of key enzymes involved in polyamine biosynthesis, specifically arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and S-adenosyl-<span>l</span>-methionine decarboxylase (SAMDC), while concurrently decreasing the activities of polyamine degrading enzymes, namely polyamine oxidase (PAO) and diamine oxidase (DAO). Additionally, melatonin elevated the levels of putrescine (Put), spermidine (Spd), and spermine (Spm) in both roots and leaves. Transcriptome analysis further indicated that melatonin upregulated genes related to polyamine biosynthesis. To further verify the role of polyamines in melatonin-induced drought tolerance, seedlings were treated with exogenous Spd or a polyamine biosynthesis inhibitor (dicyclohexylammonium sulfate, DCHA) under combined drought and melatonin conditions. Exogenous Spd produced effects similar to those of melatonin under drought stress, while DCHA abolished the melatonin-induced drought tolerance and reduced its beneficial effects on root water absorption. These findings demonstrate that polyamines are involved in melatonin-induced drought tolerance in foxtail millet, supporting the positive role of melatonin in modulating drought tolerance.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian-Related Serotonin/Melatonin Level Modulates Cisplatin Ototoxicity Susceptibility Depended on NOS3–NO Pathway","authors":"Siyu Qiu,&nbsp;Shiyi Cai,&nbsp;Rong Xie,&nbsp;Chang Liu,&nbsp;Yingzi He","doi":"10.1111/jpi.70154","DOIUrl":"10.1111/jpi.70154","url":null,"abstract":"<p>Hearing impairment is attributed to factors such as age, genetic predisposition, and environmental influences, among which environmental factors are considered modifiable. Among various environmental factors, the role of poor lifestyle habits is particularly critical, yet the specific mechanisms by which they contribute to hearing damage remain unclear. This study reveals that dysregulated hormone levels due to disrupted light exposure may significantly increase susceptibility to sensorineural hearing loss. In mice, circadian rhythm disruption was found to reduce melatonin and elevate serotonin levels in the inner ear, thereby increasing vulnerability to cisplatin-induced ototoxicity. In both in vivo and in vitro cisplatin-treatment models, we showed that combined treatment with melatonin protected hearing, reduced inner ear cell death, and preserved synaptic connections, whereas serotonin co-administration exacerbated the damage. Using small molecule–protein interaction prediction, we identified NOS3 as a potential target of both melatonin and serotonin, through which they appear to regulate the NO signaling pathway and influence hair cell ferroptosis. Finally, exogenous supplementation of NOS3 in cochlear tissues effectively mitigated cisplatin-induced hair cell damage, even under conditions of circadian rhythm disruption. These findings indicate that the melatonin/serotonin balance modulates susceptibility to sensorineural hearing loss via the NOS3–NO signaling pathway.</p>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpi.70154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventive Antiarrhythmic Properties of Melatonin in Experimental Diabetic Cardiomyopathy in Rats","authors":"Alexey O. Ovechkin,&nbsp;Mikhail A. Gonotkov,&nbsp;Alena S. Abramochkina,&nbsp;Ekaterina N. Sedyakina,&nbsp;Aleksandra V. Durkina,&nbsp;Olesya G. Bernikova,&nbsp;Ilya O. Velegzhaninov,&nbsp;Jan E. Azarov","doi":"10.1111/jpi.70153","DOIUrl":"10.1111/jpi.70153","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetes mellitus (DM) promotes cardiac arrhythmias, whereas melatonin confers antiarrhythmic effects. Cellular targets modified by DM and melatonin largely overlap, which warrants testing melatonin as an antiarrhythmic agent in DM. Herein, we evaluated electrophysiological and antiarrhythmic properties of melatonin in the experimental DM model. Experiments were performed in 41 control and 71 diabetic rats. 34 diabetic animals were treated with melatonin (orally, 10 mg/kg daily) for 1 month, and 37 diabetic animals received a placebo. Electrophysiological studies included electrocardiography, in vivo epicardial mapping with induction of ischemia-reperfusion, and patch-clamp studies in ventricular cardiomyocytes. Collagen deposition was assessed in postmortem histological studies. RT-PCR and Western blotting methods were used to estimate <i>Gja1</i> gene expression and Cx43 protein level, respectively. Melatonin treatment in diabetic animals did not influence the level of glycaemia, but mitigated the progression of myocardial fibrosis. Melatonin treatment prevented the DM-related decrease in conduction velocity, increase in duration and dispersion of repolarization, as well as corresponding changes in their electrocardiographic correlates (QRS, QT, and Tpeak-Tend intervals, respectively). Melatonin also prevented the development of reperfusion ventricular tachycardia/fibrillation. The patch-clamp studies showed that melatonin treatment significantly reduced calcium current, prevented DM-related action potential duration prolongation, but did not modify DM-related increase in sodium current. RT-PCR studies demonstrated that melatonin treatment prevented the DM-related upregulation of <i>Gja1</i> gene expression and increase in Cx43 level. Melatonin treatment of diabetic rats conferred antiarrhythmic effects associated with electrophysiological changes reflected in ECG parameters, which warrants further testing of melatonin in a clinical context.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Alleviates Sleep Disturbance-Induced Ovarian Reserve Decline by Suppressing NLRP3-Mediated Pyroptosis in Granulosa Cells","authors":"Qunying Fang,&nbsp;Zonghui Qiao,&nbsp;Fangfang Li,&nbsp;Shun Bai,&nbsp;Min Chen,&nbsp;Shengyuan Chen,&nbsp;Bo Xu,&nbsp;Baolu Shi,&nbsp;Limin Wu","doi":"10.1111/jpi.70151","DOIUrl":"10.1111/jpi.70151","url":null,"abstract":"<div>\u0000 \u0000 <p>Sleep disturbance perturbs circadian and immune homeostasis and is increasingly associated with female reproductive dysfunction, yet the underlying cellular mechanisms remain unclear. Here, we identify granulosa cell pyroptosis as a central mechanism linking sleep disturbance to ovarian reserve decline. Mendelian randomization analyses support an association between frequent sleep disorders and ovarian dysfunction, consistent with clinical evidence of reduced ovarian reserve in women with poor sleep quality. Mechanistically, sleep disturbance induces a pro-inflammatory ovarian microenvironment characterized by oxidative stress, activation of the NLRP3 inflammasome, and pyroptotic death of granulosa cells, accompanied by ultrastructural damage. Melatonin, a key circadian regulator and a potent antioxidant, suppresses NLRP3-mediated pyroptosis, alleviates oxidative stress, and preserves granulosa cell integrity. In a randomized clinical setting, melatonin supplementation partially restores ovarian reserve markers and improves reproductive outcomes. These findings define an inflammation-driven pyroptotic pathway underlying sleep disturbance–induced ovarian dysfunction and establish melatonin as a mechanistic modulator of ovarian inflammasome activation, supporting circadian-targeted strategies for preserving female reproductive health.</p></div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148100658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"78 4","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148096072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}