Journal of Pineal Research最新文献

{"title":"Analyzing the Interactions of Light and Melatonin Forcing in a Mathematical Model of the Human Circadian Oscillator","authors":"Shelby R. Stowe,&nbsp;Armelle Duston,&nbsp;Will Robinson,&nbsp;Cecilia Diniz Behn","doi":"10.1111/jpi.70056","DOIUrl":"https://doi.org/10.1111/jpi.70056","url":null,"abstract":"<div>\u0000 \u0000 <p>The pineal secretion of the hormone melatonin demonstrates a circadian (~24 h) rhythm with the onset of melatonin production at night and offset each morning under tight circadian control for entrained individuals. Melatonin exerts both acute sleep-promoting effects and phase-shifting effects on the circadian clock. Due to its hypnotic and chronobiotic (phase shifting) effects, exogenous melatonin supplements are increasingly being used as a treatment for a variety of sleep and circadian diseases and disorders. Phase shifting of the circadian clock can also be accomplished through ocular exposure to light. However, the interacting effects of light and melatonin on the circadian clock are not well understood. To analyze the dynamic behavior of both endogenous and exogenous melatonin's influence on the circadian clock, we extend a previously published mathematical model of the circadian clock to account for forcing due to both endogenous melatonin produced by the pineal gland and exogenous melatonin entering the system through ingested oral supplements. We fit model parameters using published melatonin pharmacokinetics, a melatonin suppression illuminance-response curve, and a 3-pulse 3 mg melatonin phase response curve (PRC). Simulated microscopic PRCs to light and melatonin are determined by the model fits and demonstrate a relative phase difference consistent with previous observations in experimental PRC data. Finally, we simulate a phase advancing experimental protocol utilizing both light exposure and exogenous melatonin to generate model predictions for the effects of interacting inputs to the clock. This modeling framework allows for the study of melatonin's dynamic properties and interaction with the circadian clock. Furthermore, it provides a framework for determining optimal light exposure and exogenous melatonin administration schedules to induce desired phase shifting of the circadian clock.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin as a Ripening Inhibitor: Enhancing Shelf Life and Quality in Red Banana","authors":"Anchana Kandasamy,&nbsp;Kavitha Chinnasamy,&nbsp;Suresh Kumar Paramasivam,&nbsp;Johnson Iruthayasamy","doi":"10.1111/jpi.70060","DOIUrl":"https://doi.org/10.1111/jpi.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>In climacteric fruits like banana (<i>Musa</i> spp.), ripening is driven by ethylene production and increased respiration, leading to rapid softening, quality loss, and disease susceptibility. This study was aimed to evaluate the effect of postharvest melatonin dip (1.0 mM and 1.5 mM for 15 min) on Red Banana stored under ambient and cold storage conditions. Melatonin significantly suppressed ethylene biosynthesis (Cohen's <i>d ƞ</i>² = 0.85), reduced respiration rate (<i>ƞ</i>² = 0.89), and delayed textural degradation by inhibiting cell wall-degrading enzymes (polygalacturonase, pectin methyl esterase, amylase, cellulase, and β-glucosidase) with 35.94% and 45.48% reduction in cumulative enzyme activity under ambient and cold storage, respectively. It also enhanced antioxidant enzyme activity resulting in 1.8- and 1.5-fold increases in enzyme activity in ambient and cold storage, respectively, mitigating oxidative stress and reducing anthracnose incidence. Consequently, melatonin extended shelf life by 2.67 days in ambient storage and 5.33 days in cold storage, without inducing chilling injury. These findings highlight melatonin as a natural, eco-friendly alternative, offering a sustainable strategy to enhance Red Banana storage and reduce postharvest losses. Its ability to modulate fruit metabolism, enhance stress responses, and membrane protection properties underscores its applied potential in postharvest management.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Alleviates Oxidative Stress-Induced Mitochondrial Dysfunction Through Ameliorating NAD+ Homeostasis of hDPSCs for Cell-Based Therapy","authors":"Xiu Peng,&nbsp;Li Zhao,&nbsp;Jiale Wang,&nbsp;Yinmo Zhang,&nbsp;Zihan Liu,&nbsp;Kun Wang,&nbsp;Linglin Zhang","doi":"10.1111/jpi.70058","DOIUrl":"https://doi.org/10.1111/jpi.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>Human dental pulp stem cells (hDPSCs) exhibit amazing therapeutic abilities in a variety of diseases due to their remarkable self-renewal capacity and multi-differentiation potential. However, their therapeutic potential could be weakened by various factors such as oxidative stress in cell survival microenvironment In Vivo. Here, we explored the protective effect and mechanism of melatonin (Mel) on hDPSCs transplanted in a type 1 diabetes mellitus (T1DM) rat model. Nicotinamide adenine dinucleotide (NAD⁺) metabolism and mitochondrial function were remarkably impaired in T1DM rats caused by oxidative stress, while the combination of Mel and post-hDPSCs transplantation could rebalance NAD⁺ homeostasis through regulating NAMPT-NAD⁺-SIRT1 axis. Furthermore, Mel significantly reduced intracellular and mitochondrial reactive oxygen species, and alleviated cell senescence and apoptosis of hDPSCs exposed to hydrogen peroxide through ameliorating NAD⁺ depletion and mitochondrial dysfunction. The protective role of Mel could be extremely essential to stem cells in tissue engineering and regenerative medicine.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Melatonin Production Improves Plant Metabolic Function in Short-Term Climate-Induced Stresses","authors":"Eun-Hae Kwon,&nbsp;Arjun Adhikari,&nbsp;Abdul Latif Khan,&nbsp;Eunsu Do,&nbsp;Nusrat Jahan Methela,&nbsp;Chung-Yeol Lee,&nbsp;Sang-Mo Kang,&nbsp;Kang-Mo Ku,&nbsp;Byung-Wook Yun,&nbsp;In-Jung Lee","doi":"10.1111/jpi.70052","DOIUrl":"https://doi.org/10.1111/jpi.70052","url":null,"abstract":"<p>Climate change, specifically high temperatures, can reduce soil moisture and cause hypersaline conditions, which creates an unsustainable agro-production system. Microbial symbionts associated with plants relinquish stressful conditions by producing stress-protecting substances. Melatonin is a signaling and stress-protecting molecule for plants, but is least known for microbial symbionts and their function in stress protection. Here, our study shows that the melatonin-synthesizing <i>Bacillus velezensis</i> EH151 (27.9 ng/mL at 96 h) significantly improved host plant (<i>Glycine max</i> L.) growth, biomass, photosynthesis, and reduced oxidative stress during heat and salinity stress conditions than the non-inculcated control. The EH151 symbiosis enhanced the macronutrient (P, Ca, and K) and reduced Na uptake in shoots during stress conditions. The microbial inoculation significantly expressed the <i>high-affinity K</i>^<i>+</i> <i>transporter</i>, <i>MYB transcription factor</i>, <i>Salt Overly Sensitive 1</i>, <i>Na</i>^<i>+</i><i>/H</i>^<i>+</i> <i>antiporter 2</i>, and <i>heat shock transcription factors</i> in spatio-temporal orders during heat and salinity stress (H&amp;S 1, 3, 10, and 14 h). We observed that microbial strain significantly increased the plant's endogenous abscisic acid (49.5% in H&amp;S 10 h), jasmonic acid (71% in H&amp;S 10 h), and melatonin biosynthesis (418% in H&amp;S 14 h). Metabolome map of plant defense response showed that EH151 enhanced activation of amino acid metabolism pathways (e.g., glutamate (34%) <span>L</span>-aspartate (82%), glycine (18.5%), and serine (58%) under H&amp;S 14 h compared to non-inoculation). Conversely, the free sugars and organic acids within the central carbon metabolism were significantly activated in non-inoculated combined heat and salinity stress compared to inoculated plants—suggesting lesser defense energy activated for stress tolerance. In conclusion, the current results show promising effects of the microbial abilities of melatonin that can regulate host growth and defense responses. Utilization of beneficial strains like <i>B. velezensis</i> EH151 could be the ideal strategy to improve stress tolerance and overcome the adverse impact of climate-induced abrupt changes.</p>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpi.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light and Temperature Coordinately Regulate Phytomelatonin Synthesis to Maintain Plant Morphogenesis via the COP1-HY5 Module","authors":"Zhi-Xin Xiang,&nbsp;Ying-Rui Li,&nbsp;Ning-Xin Zhang,&nbsp;Ya-Xuan Zhang,&nbsp;Ting-Ting Yuan","doi":"10.1111/jpi.70059","DOIUrl":"https://doi.org/10.1111/jpi.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Light and temperature change constantly under natural conditions and play vital roles in coordinating plant morphogenesis. However, how these two signals are integrated with endogenous signals to fine-tune plant morphology requires further investigation. Given that phytomelatonin is a multifunctional regulator connecting environmental signals and plant development, here we propose that phytomelatonin is involved in the integration of light and temperature signals. When co-treated with darkness and warm ambient temperature, the light–temperature signal showed synergistic upregulation of phytomelatonin synthesis and thus hypocotyl growth. Phytomelatonin synthesis gene <i>SEROTONIN N-ACETYLTRANSFERASE</i> (<i>SNAT</i>) was induced under constant darkness or warm temperature, reaching its peak level under the combined treatment. The <i>snat</i> mutant, with reduced phytomelatonin content and hypocotyl length, was less sensitive to darkness and warm temperature, whereas <i>35S::SNAT-GFP</i> had more phytomelatonin and longer hypocotyls than the wild type, indicating that <i>SNAT</i> is needed for light–temperature morphogenesis. Furthermore, <i>SNAT</i> expression and phytomelatonin content were reduced in <i>cop1</i> but increased in <i>hy5</i>. HY5 inhibits <i>SNAT</i> expression by binding to its promoter. The <i>hy5 snat</i> seedlings had less phytomelatonin and shorter hypocotyls than the <i>hy5</i> seedlings, along with the <i>SNAT</i> mutation in <i>35S::COP1 snat</i> seedlings reversed the phenotype of <i>35S::COP1</i>, further verifying that <i>SNAT</i> acts downstream of COP1-HY5 module. Moreover, RNA-Seq revealed that phytomelatonin is associated with light–temperature signal in controlling hypocotyl elongation-related genes. Taken together, our results showed that the light–temperature signal regulates <i>SNAT</i>-mediated phytomelatonin synthesis through COP1-HY5 module to coordinate plant morphogenesis.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise as a Synchronizer: Effects on Circadian Re-Entrainment of Core Body Temperature and Metabolism Following Light–Dark Cycle Inversion in Mice","authors":"Andrea Michele Ciorciari,&nbsp;Emanuel Irizarry,&nbsp;Angela Montaruli,&nbsp;Katja A. Lamia","doi":"10.1111/jpi.70057","DOIUrl":"https://doi.org/10.1111/jpi.70057","url":null,"abstract":"<p>Core body temperature (CBT) is a crucial marker of circadian synchrony, reflecting behavioral, metabolic, and environmental adaptations. Disruptions to CBT rhythms, as seen in shift workers or jetlag, indicate desynchronization and can lead to significant health consequences. Exercise is a potent non-photic zeitgeber that may help align circadian rhythms with external cues, but its role in re-entrainment following abrupt phase shifts remains unclear. This study investigated whether voluntary exercise accelerates the re-entrainment of CBT and metabolic rhythms in mice subjected to a 12-h light-dark cycle inversion (LDI). Fifteen C57BL/6 J mice underwent LDI and were divided into two groups. Mice in the control (CTRL) group remained sedentary throughout the experiment while mice in the other group were provided running wheels for 2 weeks after LDI. CBT was continuously monitored using implanted telemetric capsules and metabolic parameters were assessed before and 2 weeks after LDI. Mice that had access to running wheels (RW mice) initially displayed a greater disruption of CBT rhythmicity following LDI, suggesting unstructured physical activity may temporarily exacerbate misalignment, acting as a conflicting signal. Despite this, exercise accelerated recovery, as the phase of the CBT rhythm in RW mice re-aligned to the new light-dark cycle faster than that of the CTRL mice did. The phase of VO₂ rhythms in RW mice also showed trends toward faster realignment. These findings highlight the dual role of exercise as a zeitgeber, capable of both disrupting and accelerating circadian realignment depending on timing. Voluntary exercise may thus serve as an effective intervention to restore circadian synchrony and metabolic homeostasis in individuals experiencing circadian disruptions.</p>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpi.70057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IAT4, a New Indolamine N-Acetyltransferase in Saccharomyces cerevisiae Involved in Melatonin Biosynthesis","authors":"Andrés Planells-Cárcel,&nbsp;Sandra Sánchez-Martí,&nbsp;Sara Muñiz-Calvo,&nbsp;José Manuel Guillamon","doi":"10.1111/jpi.70053","DOIUrl":"https://doi.org/10.1111/jpi.70053","url":null,"abstract":"<p>Melatonin synthesis by yeast has been described on several occasions, mainly in a fermentative context. However, the genetic determinants involved in its synthesis remain undefined. Understanding melatonin synthesis in yeast is important because it can provide insights into the broader mechanisms of indolamine production, which has implications for both basic biological research and industrial applications. Although two genes with <i>N</i>-acetyltransferase (NAT) activity (<i>PAA1</i> and <i>HPA2</i>) have been identified in <i>Saccharomyces cerevisiae</i>, these genes do not seem to be major contributors to the production of melatonin and other indolamines in yeast in vivo. In this study, we identified the uncharacterized gene YDR391C as the gene encoding a protein with NAT activity, herein named <i>IAT4</i>. By comparing different substrates using the purified Iat4, we found that the <i>K</i>_<i>m</i> values were 353, 356, and 930 µM towards 5-methoxytryptamine, tryptamine, and serotonin, respectively. The substrate affinity of Iat4 towards serotonin was approximately five times higher than that reported for the previous homolog of the melatonin enzyme arylalkylamine <i>N</i>-acetyltransferase (<i>PAA1</i>), suggesting that <i>IAT4</i> could play a more significant role in melatonin biosynthesis. This enhanced affinity could lead to more efficient production of <i>N</i>-acetylserotonin, potentially improving yields in biotechnological applications. Finally, we demonstrate the conversion of serotonin into microbially-produced <i>N</i>-acetylserotonin by overexpressing <i>IAT4</i> in a serotonin-overproducing yeast strain at a titer of 14.5 mg/L. These findings represent the first steps towards the development of yeast strains optimized for the biological production of <i>N</i>-acetylserotonin and related compounds, which might aid in studying the regulatory mechanisms and functions related to melatonin biosynthesis in <i>S. cerevisiae</i> and other yeast species.</p>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpi.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Supplementation Alleviates Bone Mineral Density Decline and Circulating Oxidative Stress in Iron-Overloaded Thalassemia Patients","authors":"Pokpong Piriyakhuntorn,&nbsp;Adisak Tantiworawit,&nbsp;Mattabhorn Phimphilai,&nbsp;Tawika Kaewchur,&nbsp;Piangrawee Niprapan,&nbsp;Bhumrapee Srivichit,&nbsp;Nattayaporn Apaijai,&nbsp;Krekwit Shinlapawittayatorn,&nbsp;Nipon Chattipakorn,&nbsp;Siriporn C. Chattipakorn","doi":"10.1111/jpi.70055","DOIUrl":"https://doi.org/10.1111/jpi.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>Thalassemia patients often exhibit low bone mineral density (BMD). The iron overload associated with thalassemia elevates oxidative stress levels, leading to reduced BMD. Melatonin improves BMD in postmenopausal osteopenia, however, its effect on BMD in thalassemia patients with iron overload has not been investigated. A randomized controlled study was conducted at Hematology Clinic, Faculty of Medicine, Chiang Mai University. Thalassemia patients with osteopenia and iron overloaded condition, as indicated by BMD Z-score &lt;−2 at <span>l</span>-spine, femoral neck, or total hip, and serum ferritin level &gt; 500 μg/L were recruited in this study. Patients were randomized to receive either melatonin 20 mg/day or placebo at bedtime for 12 months. BMD was re-evaluated 12 months after interventions. Bone turnover markers (BTM), malondialdehyde (MDA as an oxidative stress marker), and pain scores were assessed at baseline, 6, and 12 months. The outcomes, including BMD, BTM, MDA, and pain scores, were evaluated in all patients. Forty-one thalassemia patients (18 males) were enrolled in the study and randomly assigned to either the melatonin group (<i>n</i> = 21) or the placebo group (<i>n</i> = 20). Characteristics of patients were not differences between groups. Mean age was 30.8 ± 6.2 years old. Thirty-three patients (80.4%) were transfusion-dependent patients. At 12 months, mean BMD at <span>l</span>-spine in melatonin group was not significantly different from placebo group (<i>p</i> = 0.069). However, <span>l</span>-spine BMD at 12 months in the melatonin group was significantly greater than baseline (<i>p</i> = 0.029). Serum levels of P1NP and MDA were significantly reduced at 6 months compared to baseline following melatonin treatment. The melatonin group experienced a notable decrease in back pain scores after 12 months compared to the initial measurements. 20 mg daily melatonin supplementation for 12 months alleviated <span>l</span>-spine BMD loss in iron-overloaded thalassemia with low BMD. Melatonin also significantly reduced circulating oxidative stress and mitigated back pain in these patients.</p>\u0000 </div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Melatonin's Effects on Hepatocyte Lipidome: A Critique of Analytical Methods","authors":"Yoshiyasu Takefuji","doi":"10.1111/jpi.70054","DOIUrl":"https://doi.org/10.1111/jpi.70054","url":null,"abstract":"","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESSION OF CONCERN: Adipocyte Differentiation Is Inhibited by Melatonin Through the Regulation of C/EBPβ Transcriptional Activity","authors":"","doi":"10.1111/jpi.70050","DOIUrl":"https://doi.org/10.1111/jpi.70050","url":null,"abstract":"<p><b>EXPRESSION OF CONCERN</b>: M. I. C. Alonso-Vale, S. B. Peres, C. Vernochet, S. R. Farmer and F. B. Lima, “Adipocyte Differentiation Is Inhibited by Melatonin Through the Regulation of C/EBPβ Transcriptional Activity,” <i>Journal of Pineal Research</i> 47, no. 3 (2009): 221-227, https://doi.org/10.1111/j.1600-079X.2009.00705.x.</p><p>This Expression of Concern is for the above article, published online on 03 September 2009 in Wiley Online Library (wileyonlinelibrary.com), and has been issued by agreement between the journal Editor-in-Chief, Gianluca Tosini; and John Wiley &amp; Sons Ltd. The Expression of Concern has been agreed due to concerns regarding the C/EBPα panel of the western blot shown in Figure 1 and the Perilipin panel in Figure 2. The authors provided an explanation and some data but this was not sufficient to resolve the issue. Therefore, the journal has decided to issue an Expression of Concern to inform and alert the readers about potential data inconsistencies in Figures 1 and 2.</p>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"77 3","pages":""},"PeriodicalIF":8.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpi.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}