Pathology International最新文献_第8页

Pseudoimmunofluorescent immunohistochemistry image analysis of phosphorylated signaling proteins in human esophageal squamous cell carcinoma tissue. 人食管鳞状细胞癌组织中磷酸化信号蛋白的假免疫荧光免疫组化图像分析。

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 Epub Date: 2024-01-23 DOI: 10.1111/pin.13407

Mari Nishio, Shuichi Tsukamoto, Takayuki Kodama, Manabu Shigeoka, Yu-Ichiro Koma, Hiroshi Yokozaki

{"title":"Pseudoimmunofluorescent immunohistochemistry image analysis of phosphorylated signaling proteins in human esophageal squamous cell carcinoma tissue.","authors":"Mari Nishio, Shuichi Tsukamoto, Takayuki Kodama, Manabu Shigeoka, Yu-Ichiro Koma, Hiroshi Yokozaki","doi":"10.1111/pin.13407","DOIUrl":"10.1111/pin.13407","url":null,"abstract":"Immunohistochemistry is primarily employed to visualize the localization of specific molecules in tissue samples. However, there is an increasing need for software-assisted quantitative assessment. In the present study, we performed inverted blue channel-based pseudoimmunofluorescence image analysis using original immunohistochemistry images. In human esophageal squamous cell carcinoma tissues, various humoral factors promote the phosphorylation of signaling proteins, including protein kinase B (Akt) and/or extracellular signal-regulated kinase 1/2 (ERK1/2), leading to tumor progression. Our method demonstrated applicability in the analysis of localized signaling proteins in histological sections. Relatively high phosphorylated Akt (p-Akt) intensity was observed in the cancer-stroma adjacent (Adj) and noncancerous regions of the superficial layer (SL). Furthermore, localized phosphorylated ERK1/2 (Thr202/Tyr204) was observed in the Adj of the SL and invasive front, distinct from the pattern of p-Akt (Ser473) and p-Akt (Thr308). In conclusion, pseudoimmunofluorescent immunohistochemistry image analysis is useful for the quantitative assessment and objective interpretation of localized signaling proteins in esophageal squamous cell carcinoma. The method can also be applied to analyze various immunohistochemistry images from diverse tissues.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139522473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterocolic granulomatous phlebitis associated with epidermal growth factor-containing fibulin-like extracellular matrix protein 1 deposition and focal amyloid properties: A case report. 肠结肠肉芽肿性静脉炎伴有含表皮生长因子的纤维蛋白样细胞外基质蛋白 1 沉积和局灶性淀粉样蛋白特性：病例报告。

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 Epub Date: 2024-01-19 DOI: 10.1111/pin.13405

Shojiro Ichimata, Akane Aikawa, Naonori Sugishita, Nagaaki Katoh, Fuyuki Kametani, Hibiki Tagawa, Yusuke Handa, Masahide Yazaki, Yoshiki Sekijima, Takashi Ehara, Naoki Nishida, Shin Ishizawa

{"title":"Enterocolic granulomatous phlebitis associated with epidermal growth factor-containing fibulin-like extracellular matrix protein 1 deposition and focal amyloid properties: A case report.","authors":"Shojiro Ichimata, Akane Aikawa, Naonori Sugishita, Nagaaki Katoh, Fuyuki Kametani, Hibiki Tagawa, Yusuke Handa, Masahide Yazaki, Yoshiki Sekijima, Takashi Ehara, Naoki Nishida, Shin Ishizawa","doi":"10.1111/pin.13405","DOIUrl":"10.1111/pin.13405","url":null,"abstract":"A woman in her 60s with rheumatoid arthritis was admitted with fever and abdominal pain. Laparoscopic examination with the differential diagnosis of peritoneal neoplasm and infection revealed granulomatous phlebitis in the resected greater omentum. Amorphous eosinophilic deposits observed in the resected tissue exhibited focal, weak positivity for Congo red but were strongly positive for thioflavin S, confirming their focal amyloid properties. Marked degeneration of elastic fibers was also evident. Electron microscopy revealed deposits around the affected elastic fibers. Immunohistochemistry revealed the deposition of epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) along with T-cell-predominant lymphocytic inflammation. The definitive diagnosis was granulomatous enterocolic lymphocytic phlebitis (ELP) associated with EFEMP1 deposition exhibiting focal amyloid properties (EFEMP1/AEFEMP1), supported by proteomics analysis. This type of vasculitis is similar to amyloid-β-related angiitis of the central nervous system. Thus, we speculate that granulomatous ELP also results from an immune response that recognizes EFEMP1/AEFEMP1 deposits as foreign material and attempts to remove them. Confirmation of EFEMP1/AEFEMP1 deposition with Congo red staining is challenging, particularly in the presence of inflammation, and warrants comprehensive evaluation.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of small intestinal occludin and ZO-1 expession in liver cirrhosis. 肝硬化患者小肠闭塞素和 ZO-1 表达的改变

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 Epub Date: 2024-01-22 DOI: 10.1111/pin.13406

Theodoros Voulgaris, Dina Tiniakos, Dimitrios Karagiannakis, Despoina Myoteri, Despoina Karandrea, Spilios Manolakopoulos, George Papatheodoridis, Jiannis Vlachogiannakos

引用次数: 0

Late relapsing testicle cancer identified with isochromosome 12p (i12p) fluorescence in situ hybridization (FISH) analysis. 通过 12p 异染色体（i12p）荧光原位杂交（FISH）分析确定的晚期复发性睾丸癌。

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 Epub Date: 2024-02-29 DOI: 10.1111/pin.13414

Teruki Isobe, Noriyasu Kawai, Maria Aoki, Toshiharu Morikawa, Masakazu Gonda, Nami Tomiyama, Takashi Nagai, Keitaro Iida, Toshiki Etani, Taku Naiki, Toyonori Tsuzuki, Takahiro Yasui

引用次数: 0

Abstracts of presentation by the winners of The Japanese Society of Pathology; Japan Pathology Award in 2024 (in program order). 日本病理学会 2024 年日本病理学奖获奖者演讲摘要（按节目顺序排列）。

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 DOI: 10.1111/pin.13401

引用次数: 0

Molecular pathological demonstration of an unusual angiosarcoma-like dedifferentiation pattern in hepatocellular carcinoma. 分子病理学显示肝细胞癌存在不寻常的血管肉瘤样去分化模式。

IF 2.2 4区医学

Pathology International Pub Date : 2024-03-01 Epub Date: 2024-02-06 DOI: 10.1111/pin.13412

Keigo Murakami, Taito Itoh, Yasunobu Okamura, Kengo Kinoshita, Hideki Ota, Michiaki Unno, Takashi Kamei, Toru Furukawa

引用次数: 0

HER2 staining of breast cancer is reduced under the current protocol of Ventana PATHWAY anti-HER-2/neu (4B5). 在目前的 Ventana PATHWAY 抗 HER-2/neu (4B5) 方案中，乳腺癌的 HER2 染色减少。

IF 2.2 4区医学

Pathology International Pub Date : 2024-02-01 Epub Date: 2024-01-10 DOI: 10.1111/pin.13404

Yoshiya Horimoto, Hiroko Onagi, Junichiro Watanabe, Takuo Hayashi

引用次数: 0

CSF3R-, ASXL1-, and SETBP1-mutated chronic neutrophilic leukemia with monoclonal gammopathy of undetermined significance in a patient who developed severe ascites due to sinusoidal neutrophilic infiltration. CSF3R、ASXL1 和 SETBP1 基因突变的慢性中性粒细胞白血病伴有意义不明的单克隆抗体病，患者因窦状中性粒细胞浸润而出现严重腹水。

IF 2.2 4区医学

Pathology International Pub Date : 2024-02-01 Epub Date: 2023-12-05 DOI: 10.1111/pin.13394

Naonori Harada, Daiki Mukai, Yuto Izuta, Ikumi Shibano, Yusuke Kizawa, Hiroshi Shiragami, Nobuhiko Ohnishi, Naoki Hosaka, Atsuko Mugitani

引用次数: 0

Is the endomyocardial biopsy in giant cell myocarditis of some help for the rapid indication for heart transplantation? 巨细胞心肌炎的心内膜活检是否有助于快速确定心脏移植指征？

IF 2.2 4区医学

Pathology International Pub Date : 2024-02-01 Epub Date: 2023-12-27 DOI: 10.1111/pin.13400

Andrea Marzullo, Gabriella Serio, Gerardo Cazzato, Marialessandra Capuzzolo, Carla Nardelli, Cecilia Salzillo

引用次数: 0

Decoding the DNA methylome of central nervous system tumors: An emerging modality for integrated diagnosis. 解码中枢神经系统肿瘤的 DNA 甲基组：综合诊断的新兴模式。

IF 2.2 4区医学

Pathology International Pub Date : 2024-02-01 Epub Date: 2024-01-15 DOI: 10.1111/pin.13402

Kaishi Satomi, Koichi Ichimura, Junji Shibahara

{"title":"Decoding the DNA methylome of central nervous system tumors: An emerging modality for integrated diagnosis.","authors":"Kaishi Satomi, Koichi Ichimura, Junji Shibahara","doi":"10.1111/pin.13402","DOIUrl":"10.1111/pin.13402","url":null,"abstract":"The definitive diagnosis and classification of individual cancers are crucial for patient care and cancer research. To achieve a robust diagnosis of central nervous system (CNS) tumors, a genotype-phenotype integrated diagnostic approach was introduced in recent versions of the World Health Organization classification, followed by the incorporation of a genome-wide DNA methylome-based classification. Microarray-based platforms are widely used to obtain DNA methylome data, and the German Cancer Research Center (Deutsches Krebsforschungszentrum [DKFZ]) has a webtool for a DNA methylation-based classifier (DKFZ classifier). Integration of DNA methylome will further enhance the precision of CNS tumor classification, especially in diagnostically challenging cases. However, in the clinical application of DNA methylome-based classification, challenges related to data interpretation persist, in addition to technical caveats, regulations, and limited accessibility. Dimensionality reduction (DMR) can complement integrated diagnosis by visualizing a profile and comparing it with other known samples. Therefore, DNA methylome-based classification is a highly useful research tool for auxiliary analysis in challenging diagnostic and rare disease cases, and for establishing novel tumor concepts. Decoding the DNA methylome, especially by DMR in addition to DKFZ classifier, emphasizes the capability of grasping the fundamental biological principles that provide new perspectives on CNS tumors.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0