Pathology International最新文献_第8页

Breast cancer during pregnancy of Luminal A type overexpressed CXCL13. 妊娠期 A 型乳腺癌过表达 CXCL13。

IF 2.5 4区医学

Pathology International Pub Date : 2024-10-01 Epub Date: 2024-08-28 DOI: 10.1111/pin.13474

Fumi Nozaki, Yoko Nakanishi, Tomoyuki Tanino, Tomohiro Ochi, Reika In, Yuka Kajiura, Kumiko Kida, Junko Takei, Atsushi Yoshida, Naoki Kanomata, Atsuko Kitano, Hideko Yamauchi, Shinobu Masuda

{"title":"Breast cancer during pregnancy of Luminal A type overexpressed CXCL13.","authors":"Fumi Nozaki, Yoko Nakanishi, Tomoyuki Tanino, Tomohiro Ochi, Reika In, Yuka Kajiura, Kumiko Kida, Junko Takei, Atsushi Yoshida, Naoki Kanomata, Atsuko Kitano, Hideko Yamauchi, Shinobu Masuda","doi":"10.1111/pin.13474","DOIUrl":"10.1111/pin.13474","url":null,"abstract":"Pregnancy-associated breast cancer has been increasing. In this study, we analyzed patients with breast cancer that occurred during pregnancy (PrBC) and compared their genetic profiles with those of patients with breast cancer that did not occur during pregnancy, within 1 year after childbirth nor during lactation (non-PrBC). We performed gene expression analyses of patients with PrBC and non-PrBC using microarrays and qRT-PCR. Microarray analysis showed that 355 genes were upregulated in the luminal-type PrBC group compared to those in the non-PrBC group. The C-X-C motif chemokine ligand 13 (CXCL13) gene was the most upregulated in the PrBC group compared to that in the non-PrBC group, especially in the luminal A-type (p = 0.016). This result was corroborated by the qRT-PCR analysis of microdissected cancer cells (p < 0.001). A negative correlation was observed between CXCL13 and estrogen receptor 1 (ESR1) mRNA expression levels in luminal A-type breast carcinoma (p < 0.001). Our results provide clues for a better understanding of breast cancer pathogenesis during pregnancy.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"592-603"},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing. 利用纳米孔测序法检测到荧光原位杂交阴性的关节内肌样脂肪肉瘤，其中有涉及 EWSR1::DDIT3 的复杂重排。

IF 2.5 4区医学

Pathology International Pub Date : 2024-10-01 Epub Date: 2024-07-29 DOI: 10.1111/pin.13468

Naohiro Makise, Jason Lin, Hajime Kageyama, Naoki Takeda, Mariko Oikawa, Takahiro Sugiyama, Hidetada Kawana, Akinobu Araki, Hideyuki Kinoshita, Hiroto Kamoda, Yoko Hagiwara, Akihiko Yoshida, Tsukasa Yonemoto, Masahito Kawazu, Makiko Itami

{"title":"Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing.","authors":"Naohiro Makise, Jason Lin, Hajime Kageyama, Naoki Takeda, Mariko Oikawa, Takahiro Sugiyama, Hidetada Kawana, Akinobu Araki, Hideyuki Kinoshita, Hiroto Kamoda, Yoko Hagiwara, Akihiko Yoshida, Tsukasa Yonemoto, Masahito Kawazu, Makiko Itami","doi":"10.1111/pin.13468","DOIUrl":"10.1111/pin.13468","url":null,"abstract":"Myxoid liposarcoma (MLPS) is a rare sarcoma, typically arising in deep soft tissues during the fourth to fifth decades of life. Histologically, MLPS is composed of uniform oval cells within a background of myxoid stroma and chicken-wire capillaries. Genetically, MLPS is characterized by the FUS/EWSR1::DDIT3 fusion gene, which generally results from balanced interchromosomal translocation and is detectable via DDIT3 break-apart fluorescence in situ hybridization (FISH). Here, we report an unusual intra-articular MLPS case, negative for DDIT3 break-apart FISH but positive for EWSR1::DDIT3. An 18-year-old female was referred to our hospital complaining of an intra-articular mass in the right knee joint. Histologically, the tumor was mainly composed of mature adipocytes, brown fat-like cells, and lipoblasts. Nanopore sequencing detected DNA rearrangements between EWSR1 and DDIT3 and clustered complex rearrangements involving multiple chromosomes, suggesting chromoplexy. Methylation classification using random forest, t-distributed stochastic neighbor embedding, and unsupervised hierarchical clustering correctly classified the tumor as MLPS. The copy number was almost flat. The TERT promoter C-124T was also detected. This report highlights, for the first time, the potential value of a fast and low-cost nanopore sequencer for diagnosing sarcomas.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"604-610"},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathology of peritonitis due to infectious mononucleosis with background Chlamydia trachomatis infection: A case report and literature review. 传染性单核细胞增多症合并沙眼衣原体感染所致腹膜炎的组织病理学：病例报告和文献综述。

IF 2.5 4区医学

Pathology International Pub Date : 2024-10-01 Epub Date: 2024-07-26 DOI: 10.1111/pin.13469

Yuto Kaimi, Tomoaki Naka, Hiroshi Yoshida, Masaya Uno, Akiko Miyagi Maeshima

{"title":"Histopathology of peritonitis due to infectious mononucleosis with background Chlamydia trachomatis infection: A case report and literature review.","authors":"Yuto Kaimi, Tomoaki Naka, Hiroshi Yoshida, Masaya Uno, Akiko Miyagi Maeshima","doi":"10.1111/pin.13469","DOIUrl":"10.1111/pin.13469","url":null,"abstract":"Epstein-Barr virus (EBV) is a major cause of infectious mononucleosis (IM), characterized by fever, fatigue, sore throat, lymphadenopathy, atypical lymphocytosis, and elevated liver enzymes. However, ascites is a rare complication associated with IM. We present a rare case of IM with ascites and peritonitis in a patient who underwent a peritoneal biopsy. A 20-year-old woman presented with fatigue and abdominal distension. Laboratory examination revealed atypical lymphocytes in peripheral blood (54%) and elevated liver enzymes. EBV serological tests revealed a recent primary infection (EBV VCA IgM 1:160). Computed tomography revealed moderate ascites and peritonitis. Adenocarcinoma was suspected based on the ascites' cytology. Considering possible complications of IM and adenocarcinoma, a laparoscopic biopsy was performed. Histological findings of biopsy specimens from the peritoneum, omentum, and fimbria of the fallopian tube demonstrated severe inflammatory cell infiltration and focal aggregation of large EBV-encoded RNA-1 (EBER1)-positive B cells, mimicking EBV-positive polymorphous B-cell lymphoproliferative disorder. Furthermore, intracytoplasmic inclusion bodies of Chlamydia trachomatis were observed by immunohistochemistry. Real-time polymerase chain reaction detected C. trachomatis in cervical secretions. Two months after laparoscopy, ascites decreased, and the diagnosis was IM-associated peritonitis with C. trachomatis infection. IM should be considered as a differential diagnosis in young patients with ascites.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"611-617"},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An autopsy case of invasive intraductal papillary mucinous neoplasm (IPMN) of the pancreas showing vascular intimal carcinomatosis 一例显示血管内膜癌变的胰腺浸润性导管内乳头状黏液瘤（IPMN）尸检病例

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-18 DOI: 10.1111/pin.13482

Manayu Shiina, Shinichi Ban, Miki Asahina, Shigeru Matsui

引用次数: 0

Bilateral primary angiosarcoma of the breast after reduction mammoplasty: An extremely rare case and literature review 乳房缩小术后的双侧原发性血管肉瘤：一个极其罕见的病例和文献综述

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-13 DOI: 10.1111/pin.13483

Cansu Sönmez, Erhan Ekren, Yaşar Kaan Akgök, Berfin Ekin Gözükara Yıldız, Sibel Aydın, Meryem Günay Gürleyik, Fügen Vardar Aker

引用次数: 0

Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue 直接快速猩红染色对诊断嗜酸性粒细胞结肠炎的临床意义：以结肠粘膜组织中嗜酸性粒细胞脱颗粒为重点的比较研究

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-11 DOI: 10.1111/pin.13475

Mina Ikeda, Hiroyuki Kato, Satoshi Arakawa, Takashi Kobayashi, Senju Hashimoto, Yoshiaki Katano, Ken‐ichi Inada, Yuka Kiriyama, Takuma Ishihara, Satoshi Yamamoto, Yukio Asano, Akihiko Horiguchi

{"title":"Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue","authors":"Mina Ikeda, Hiroyuki Kato, Satoshi Arakawa, Takashi Kobayashi, Senju Hashimoto, Yoshiaki Katano, Ken‐ichi Inada, Yuka Kiriyama, Takuma Ishihara, Satoshi Yamamoto, Yukio Asano, Akihiko Horiguchi","doi":"10.1111/pin.13475","DOIUrl":"https://doi.org/10.1111/pin.13475","url":null,"abstract":"This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi‐colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high‐power field). However, this method is not useful in the left hemi‐colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi‐colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi‐colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":"6 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giant inflammatory polyposis in Crohn's disease mimicking recurrent obstructing colon cancer 模仿复发性梗阻性结肠癌的克罗恩病巨型炎性息肉病

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-10 DOI: 10.1111/pin.13479

Christopher Holt, David E. Elliott, Yiqin Xiong

引用次数: 0

Standardization of CD30 immunohistochemistry staining among three automated immunostaining platforms. 三种自动免疫染色平台的 CD30 免疫组化染色标准化。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-08-22 DOI: 10.1111/pin.13472

Masafumi Seki, Akira Satou, Renji Funato, Tomoko Tamaki, Naoki Wada, Norihiro Nakada, Hirofumi Matsumoto, Iwao Nakazato, Eriko Wada, Kaneko Sakurai, Toyonori Tsuzuki, Kennosuke Karube

{"title":"Standardization of CD30 immunohistochemistry staining among three automated immunostaining platforms.","authors":"Masafumi Seki, Akira Satou, Renji Funato, Tomoko Tamaki, Naoki Wada, Norihiro Nakada, Hirofumi Matsumoto, Iwao Nakazato, Eriko Wada, Kaneko Sakurai, Toyonori Tsuzuki, Kennosuke Karube","doi":"10.1111/pin.13472","DOIUrl":"10.1111/pin.13472","url":null,"abstract":"The identification of CD30 expression by immunohistochemistry is essential for the treatment of lymphomas using an antibody-drug conjugate targeting CD30. However, no standardized protocol for CD30 staining has been available. In this study, we compared three common automated immunostaining platforms {Bond III (B III), Dako Omnis (DO) and Ventana BenchMark ULTRA (VBMU)}. A primary antibody for CD30, the Ber-H2 clone, was diluted 50- to 400-fold for B III and DO, and ready-to-use antibody was used for VBMU. An enhancement step using a linker was introduced in all protocols. First, several candidate dilutions were selected for each platform by staining six cases. These candidate conditions were then confirmed with 60 cases of various types of peripheral T-cell lymphomas (PTCLs). The concordance rates of CD30 expression among platforms differed depending on cutoff values and antibody dilutions, except for anaplastic large cell lymphoma. The concordance rates among three platforms in the evaluation of \"positive\" or \"negative\" were 100% and 97% when the cutoff values were 1% and 10% respectively, if using 400-diluted antibody in B III and 100-diluted antibody in DO. This study demonstrated the feasibility of equalizing CD30 staining of PTCLs among different platforms by adjusting protocols.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"530-537"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-calcifying Langerhans cell-rich myxoid squamous odontogenic neoplasm without amyloid: A seemingly amyloid-negative calcifying epithelial odontogenic tumor. 不含淀粉样蛋白的非钙化朗格汉斯细胞丰富肌样鳞状牙源性肿瘤：一种看似淀粉样蛋白阴性的钙化上皮性牙源性肿瘤。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-07-31 DOI: 10.1111/pin.13470

Fumio Ide, Shinnichi Sakamoto, Yoko Tateishi, Hiroyuki Hayashi, Takayuki Ohsawa, Yumi Ito, Kentaro Kikuchi

引用次数: 0

FHL1: A novel diagnostic marker for papillary thyroid carcinoma. FHL1：甲状腺乳头状癌的新型诊断标记物。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI: 10.1111/pin.13467

Yeting Zeng, Dehua Zeng, Xingfeng Qi, Hanxi Wang, Xuzhou Wang, Xiaodong Dai, Lijuan Qu

{"title":"FHL1: A novel diagnostic marker for papillary thyroid carcinoma.","authors":"Yeting Zeng, Dehua Zeng, Xingfeng Qi, Hanxi Wang, Xuzhou Wang, Xiaodong Dai, Lijuan Qu","doi":"10.1111/pin.13467","DOIUrl":"10.1111/pin.13467","url":null,"abstract":"Although there are clear morphologic criteria for the diagnosis of papillary thyroid carcinoma (PTC), when the morphology is untypical or overlaps, accurate diagnostic indicators are necessary. Since few studies investigated the role of down-regulated genes in PTC, this article aims to further explore the molecular markers associated with PTC. We conducted bioinformatics analysis of gene microarrays of PTC and normal adjacent tissues. Besides, quantitative real-time quantitative polymerase chain reaction array and immunohistochemical staining were used to investigate the expression of the major down-regulated genes. The results indicated that several important down-regulated genes, including TLE1, BCL2, FHL1, GHR, KIT, and PPARGC1A were involved in the process of PTC. Compared to normal adjacent tissues, the mRNA expression of the major genes was down-regulated in PTC (p＜0.05). Immunohistochemically, FHL1 shows negative or low expression in PTC tissues (p＜0.05). BCL2 did not show a significant difference between PTC and normal thyroid tissues (p > 0.05). TLE1, KIT, PPARGC1A and GHR showed negative expression in both tumor and normal tissues. These results suggested that FHL1 could serve as a novel tumor marker for precise diagnosis of PTC.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"520-529"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0