Pathology International最新文献_第9页

Distribution of proteinase K-resistant anti-α-synuclein immunoreactive axons in the cardiac plexus is unbiased to the left ventricular anterior wall. 抗α-突触核蛋白免疫反应性轴突在心脏丛的分布与左心室前壁无关。

IF 2.2 4区医学

Pathology International Pub Date : 2024-01-01 Epub Date: 2023-12-01 DOI: 10.1111/pin.13389

Nei Fukasawa, Miku Maeda, Yoshifumi Sugiyama, Takahiro Fukuda, Masayuki Shimoda

{"title":"Distribution of proteinase K-resistant anti-α-synuclein immunoreactive axons in the cardiac plexus is unbiased to the left ventricular anterior wall.","authors":"Nei Fukasawa, Miku Maeda, Yoshifumi Sugiyama, Takahiro Fukuda, Masayuki Shimoda","doi":"10.1111/pin.13389","DOIUrl":"10.1111/pin.13389","url":null,"abstract":"Lewy body disease (LBD) is characterized by the appearance of Lewy neurites and Lewy bodies, which are predominantly composed of α-synuclein. Notably, the cardiac plexus (CP) is one of the main targets of LBD research. Although previous studies have reported obvious differences in the frequency of Lewy body pathology (LBP) in the CP, none of them have confirmed whether LBP preferably appears in any part of the CP. Thus, we aimed to clarify the emergence and/or propagation of LBP in the CP. In this study, 263 consecutive autopsy cases of patients aged ≥50 years were included, with one region per case selected from three myocardial perfusion areas (MPAs) and subjected to proteinase K and then immunohistochemically stained with anti-α-synuclein antibodies to assess LBP. We stained all three MPAs in 17 cases with low-density LBP and observed the actual distribution of LBP. LBP were identified in the CP in 20.2% (53/263) of patients. Moreover, we found that LBP may appear in only one region of MPAs, mainly in the young-old group (35.3% (6/17) of patients). These findings suggest that it is possible to underestimate LBP in the CP, especially in the young-old group, by restricting the search to only one of the three MPAs.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138461506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What is the role of membrane-located epithelial cell transforming sequence 2 at the interphase of lung adenocarcinoma cells? 膜定位上皮细胞转化序列 2 在肺腺癌细胞间期的作用是什么？

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-20 DOI: 10.1111/pin.13397

Yoshihiko Murata, Ai Yoshida, Yuko Minami, Zeinab Kosibaty, Daisuke Matsubara, Masayuki Noguchi

引用次数: 0

Epidemiological study of the subtype frequency of systemic amyloidosis listed in the Annual of the Pathological Autopsy Cases in Japan 日本病理解剖病例年鉴》所列全身性淀粉样变性亚型频率的流行病学研究

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-13 DOI: 10.1111/pin.13395

Aina Yamaguchi, Masayoshi Tasaki, Mitsuharu Ueda, Yukio Ando, Hironobu Naiki

{"title":"Epidemiological study of the subtype frequency of systemic amyloidosis listed in the Annual of the Pathological Autopsy Cases in Japan","authors":"Aina Yamaguchi, Masayoshi Tasaki, Mitsuharu Ueda, Yukio Ando, Hironobu Naiki","doi":"10.1111/pin.13395","DOIUrl":"https://doi.org/10.1111/pin.13395","url":null,"abstract":"Clinical presentation of systemic amyloidosis differs among subtypes, and accurate subtype classification is important for choosing the treatment. Amyloid transthyretin (ATTR) amyloidosis was the predominant among the recently consulted amyloidosis cases in Japan. To reveal the latest subtype frequency of systemic amyloidosis among autopsy cases in Japan. We analyzed systemic amyloidosis cases autopsied from January 2017 to December 2018, that were listed in the Annuals of the Pathological Autopsy Cases in Japan, Volumes 60 and 61. When the subtype was unclear, we performed a questionnaire survey, immunohistochemistry with in-house rabbit polyclonal anti-κ116–133, anti-λ118–134, and anti-transthyretin115–124 antibodies, and proteomic analysis. Out of 481 systemic amyloidosis cases listed in the Annuals, 411 cases were available for analysis (85.4%). We classified 399 of these systemic amyloidosis cases. ATTR was the most common subtype (44.4%, n = 177), followed by amyloid immunoglobulin light chain (AL) (38.8%, n = 155). Amyloid A and amyloid β2-microglobulin were 9.3% (n = 37) and 6.0% (n = 24), respectively. Double deposition of amyloid was identified in 1.6% (n = 6). In 168 cases (42.1%), systemic amyloidosis was the main cause of death. Of these cases, AL was the most common subtype (47.6%, n = 80), followed by ATTR (41.1%, n = 69). ATTR is the most predominant subtype among the current autopsy cases in Japan.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138631938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal for combined macroscopic and microscopic on-site evaluation (cMOSES) of fresh tissue from liver tumor biopsies for histopathological diagnosis and comprehensive genomic panel testing. 关于对肝肿瘤活检组织的新鲜组织进行宏观和微观联合现场评估（cMOSES）以进行组织病理学诊断和综合基因组面板测试的建议。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-09-13 DOI: 10.1111/pin.13377

Tami Nagatani, Yoji Wani, Soichiro Fushimi, Yu Matsuo, Shiho Murakami, Toshifumi Tada, Shinichiro Nakamura, Hirofumi Inoue, Maki Tanioka, Hiroyuki Okada, Akira Hirasawa

引用次数: 0

Endometrial gastric-type mucinous carcinoma: A clinicopathological study of an unfavorable histological type of endometrial carcinoma. 子宫内膜胃型粘液癌：对一种不利组织学类型子宫内膜癌的临床病理学研究。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-09-06 DOI: 10.1111/pin.13376

Fumi Kawakami, Ken Yamaguchi, Sachiko Minamiguchi, Tamotsu Sudo, Takanori Hirose, Norihiro Teramoto, Yoshiki Mikami

引用次数: 0

Pulmonary interstitial glycogenosis in Birt-Hogg-Dubé syndrome-associated lung cysts: A new insight into the pathogenesis? Birt-Hogg-Dubé综合征相关肺囊肿的肺间质糖原增多症：发病机制的新见解？

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-10-11 DOI: 10.1111/pin.13383

Riken Kawachi, Yukio Nakatani, Mitsuko Furuya, Naoya Nakamura, Yusuke Kondo, Yoji Nagashima, Tomohiro Nakayama, Masahiro Okada, Hiroyuki Sakurai, Shinobu Masuda

{"title":"Pulmonary interstitial glycogenosis in Birt-Hogg-Dubé syndrome-associated lung cysts: A new insight into the pathogenesis?","authors":"Riken Kawachi, Yukio Nakatani, Mitsuko Furuya, Naoya Nakamura, Yusuke Kondo, Yoji Nagashima, Tomohiro Nakayama, Masahiro Okada, Hiroyuki Sakurai, Shinobu Masuda","doi":"10.1111/pin.13383","DOIUrl":"10.1111/pin.13383","url":null,"abstract":"Multiple lung cysts are one of the major features of Birt-Hogg-Dubé syndrome (BHD), but little is known about their nature and pathogenesis. We report a case of a woman diagnosed with BHD lung cysts who exhibited pulmonary interstitial glycogenosis (PIG), a mesenchymal abnormality hitherto undescribed in this disease, in specimens resected at 14 and 29 years of age. Histopathologically, oval to spindle clear cells were seen in the subepithelial interstitial tissue of septal structures and the walls of the cysts. They had abundant periodic acid-Schiff-positive cytoplasmic glycogen. Immunohistochemically, these cells were positive for a few markers of mesenchymal stem cell-like lineage, including vimentin, CD44, and CD10, and negative for markers of epithelial or specific mesenchymal differentiation; these results were consistent with the reported immunophenotype of PIG cells. These PIG cells were more abundant in her specimen at age 14 years than in the second specimen from adulthood. The present case suggests that BHD lung cysts belong to a group of pulmonary developmental disorders characterized by combined PIG and alveolar simplification/cystic change. Disorders with PIG may persist until adulthood and may be of clinical and pathological significance.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41208419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment. 致谢。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 DOI: 10.1111/pin.13386

引用次数: 0

Mechanisms underlying retardation of aging by dietary energy restriction. 饮食能量限制延缓衰老的机制。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-11-17 DOI: 10.1111/pin.13387

Isao Shimokawa

{"title":"Mechanisms underlying retardation of aging by dietary energy restriction.","authors":"Isao Shimokawa","doi":"10.1111/pin.13387","DOIUrl":"10.1111/pin.13387","url":null,"abstract":"Moderate restriction of dietary energy intake, referred to here as dietary restriction (DR), delays aging and extends lifespan in experimental animals compared with a diet of ad libitum feeding (AL) control animals. Basic knowledge of the mechanisms underlying the effects of DR could be applicable to extending the healthspan in humans. This review highlights the importance of forkhead box O (FoxO) transcription factors downstream of the growth hormone-insulin-like growth factor 1 signaling in the effects of DR. Our lifespan studies in mice with heterozygous Foxo1 or Foxo3 gene knockout indicated differential roles of FoxO1 and FoxO3 in the tumor-inhibiting and life-extending effects of DR. Subsequent studies suggested a critical role of FoxO3 in metabolic and mitochondrial bioenergetic adaptation to DR. Our studies also verified hypothalamic neuropeptide Y (Npy) as a vital neuropeptide showing pleiotropic and sexually dimorphic effects for extending the healthspan in the context of nutritional availability. Npy was necessary for DR to exert its effects in male and female mice; meanwhile, under AL conditions, the loss of Npy prevented obesity and insulin resistance only in female mice. Overnutrition disrupts FoxO- and Npy-associated metabolic and mitochondrial bioenergetic adaptive processes, causing the acceleration of aging and related diseases.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136398604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of formalin fixation duration on RNA quality and quantity from formalin-fixed paraffin-embedded hepatocellular carcinoma tissues. 福尔马林固定时间对福尔马林固定石蜡包埋的肝细胞癌组织RNA质量和数量的影响。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-11-07 DOI: 10.1111/pin.13385

Kenji Amemiya, Yosuke Hirotsu, Yuki Nagakubo, Hitoshi Mochizuki, Toshio Oyama, Masao Omata

{"title":"Influence of formalin fixation duration on RNA quality and quantity from formalin-fixed paraffin-embedded hepatocellular carcinoma tissues.","authors":"Kenji Amemiya, Yosuke Hirotsu, Yuki Nagakubo, Hitoshi Mochizuki, Toshio Oyama, Masao Omata","doi":"10.1111/pin.13385","DOIUrl":"10.1111/pin.13385","url":null,"abstract":"Analyzing RNA samples from formalin-fixed paraffin-embedded (FFPE) tissues is essential for precision medicine. We investigated RNA quantity and quality from FFPE tumor tissues fixed in formalin for various times and compared sequencing metrics from next-generation sequencing (NGS). Hepatocellular carcinoma (HCC) tissues were fixed in 10% neutral buffered formalin (1-240 h) and FFPE blocks were prepared. Total RNA was extracted, and the quantity and quality were assessed using the NanoDrop, Qubit and Bioanalyzer. After preparing sequencing libraries, NGS was performed on the Oncomine Dx Multi-CDx system. Total RNA yields of all samples met the threshold required for NGS, but longer fixation times resulted in decreased total RNA and long RNA fragment (>200 nt) yields. NGS analysis showed fewer sequencing reads of internal control genes from RNA with longer fixation times. RNA extracted from FFPE blocks stored for 500 days had reduced RNA yield and quality compared with RNA obtained from FFPE blocks prepared immediately. In conclusion, short and over-fixation should be avoided because of their negative impact on sequencing quality. Fixation process should be finished promptly within recommended guidelines (6-72 h) for cancer patients.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71484698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endocrine cell generation in obstructive chronic pancreatitis in humans: A phenomenon of acinar cell plasticity. 人类阻塞性慢性胰腺炎的内分泌细胞生成：腺泡细胞可塑性现象。

IF 2.2 4区医学

Pathology International Pub Date : 2023-12-01 Epub Date: 2023-10-03 DOI: 10.1111/pin.13381

Stratigoula Sakellariou, Kostas Palamaris, Nikolaos Kavantzas, Evangelos Felekouras, Ioanna Delladetsima

引用次数: 0