PeerJ最新文献

{"title":"Temporal and spatial distribution of histone acetylation in mouse molar development.","authors":"Wen Du, Wanyi Luo, Liwei Zheng, Xuedong Zhou, Wei Du","doi":"10.7717/peerj.19215","DOIUrl":"10.7717/peerj.19215","url":null,"abstract":"<p><p>Histone acetylation is one of the most widely studied histone modification, regulating a variety of biological activities like organ development and tumorigenesis. However, the role of histone acetylation in tooth development is poorly understood. Using the mouse molar as a model, we mapped the distribution patterns of histone H3 and H4, as well as their corresponding acetylation sites during tooth formation in order to unveil the connection between histone acetylation modification and tooth development. Moreover, key histone acetyltransferases and histone deacetylases were detected in both epithelial and mesenchymal cells during tooth development by scRNA-seq and immunohistochemistry. These results suggest that histone acetylation modification functions as an important mechanism in tooth development at different stages.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19215"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different light intensities on lettuce growth, yield, and energy consumption optimization under uniform lighting conditions.","authors":"Jun Zou, Shipeng Luo, Mingming Shi, Dawei Wang, Wenbin Liu, Yan Shen, Xiaotao Ding, Yuping Jiang","doi":"10.7717/peerj.19229","DOIUrl":"10.7717/peerj.19229","url":null,"abstract":"<p><p>Vertical farming is an advanced form of modern agriculture, but it involves high energy consumption when providing supplemental lighting for crops. The research designed an automated lighting detection device and explored its application in vertical farms. It comprehensively investigated the effects of different red-blue light intensities on two lettuces (<i>Lactuca sativa</i> L. cv. 'Spanish green' and 'Butterhead') varieties under highly uniform supplemental lighting conditions. The research encompasses investigations into lettuce morphological parameters, photosynthetic physiology, productivity, and energy consumption. The experimental light quality and photoperiod were set at (R/B = 4, 16 h/d), with light intensity ranging from 125 to 300 μmol/m²/s. The experiment indicates that with the increase in light intensity, the yield of both lettuce varieties also increases. Furthermore, lettuce exhibits a significant increase in leaf amplitude from day 20 to day 25 of growth. At 300 μmol/m²/s, Butterhead lettuce demonstrates optimal fresh weight and productivity ratio. As for Spanish green lettuce, the productivity ratio at 200 μmol/m²/s is only 2.11% lower than at 300 μmol/m²/s. Under the cultivation conditions of R/B = 4 and 16 h/d, a light intensity of 300 μmol/m²/s is suitable for the production of Butterhead lettuce, in comparison, a light intensity of 200 μmol/m²/s is suitable for the production of Spanish green lettuce.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19229"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating exercise therapies in adolescent idiopathic scoliosis: a systematic review with Bayesian network meta-analysis.","authors":"Yang Jiang, Hao Peng, Yanping Song, Li Huang, Hongbo Chen, Pengcheng Li, Guoshun Yang, Yueyu Song, Qigang Chen, Na Yao","doi":"10.7717/peerj.19175","DOIUrl":"10.7717/peerj.19175","url":null,"abstract":"<p><strong>Background: </strong>Exercise therapy represents a financially prudent and readily applicable intervention that has gained considerable traction in the treatment of adolescent idiopathic scoliosis (AIS) in recent years. Nevertheless, a definitive agreement on the superiority of one method over another remains elusive.</p><p><strong>Methods: </strong>A comprehensive search was performed across the PubMed, Cochrane Library, Embase, and Web of Science databases for randomized controlled trials pertaining to exercise and AIS, concluding on August 20, 2024. Four independent researchers conducted a thorough review of the literature, engaged in meticulous data extraction, and assessed the risk of bias. A Bayesian network meta-analysis was performed utilizing the R programming language (version 4.3.4) and MetaInsight tool (version V6.0.1).</p><p><strong>Results: </strong>A total of 23 studies were incorporated into the analysis, encompassing 1,289 participants with AIS. Compared to the control group, the primary meta-analysis showed that both SchrothCo and physiotherapeutic scoliosis-specific exercises (PSSE) modalities significantly improved Cobb angle (MD = -4.79, 95% CI [-8.56 to -1.11] MD = -3.11, 95% CI [-5.27 to -0.9]), axial trunk rotation (ATR) (MD = -3.03, 95% CI [-4.68 to -1.46]; MD = -2.37, 95% CI [-3.59 to -1.14]), and SRS-22 scores (MD = 0.66, 95% CI [0.39-0.94]; MD = 0.44, 95% CI [0.22-0.66]). The secondary meta-analysis showed that within the PSSE modality, Schroth therapy significantly reduced Cobb angle (MD = -2.3, 95% CI [-5.45 to -0.66]), while in the SchrothCo modality, both Schroth + Core and Schroth + Hippotherapy significantly improved Cobb angle (MD = -5.27, 95% CI [-14.15 to -3.5]). For ATR, Schroth therapy within PSSE (MD = -2.79, 95% CI [-6.4 to -0.1]), and Schroth + Core (MD = -4.03, 95% CI [-9.37 to -0.98]), Schroth + Sling (MD = -3.12, 95% CI [-10.05 to -2.94]), and Schroth + Hippotherapy (MD = -3.39, 95% CI [-10.29 to -2.84]) within the SchrothCo modality all showed significant reductions. Significant differences in SRS-22 scores were found only in the PSSE modality with Schroth therapy (MD = 0.48, 95% CI [0.02-0.9]) and in the SchrothCo modality with Schroth + Core (MD = 0.79, 95% CI [0.13-1.43]).</p><p><strong>Conclusions: </strong>According to the latest findings, the integration of the Schroth method with core stabilization training (Schroth + Core) is regarded as the optimal strategy for addressing AIS. The integration of core stabilization training with the Schroth method reveals a markedly enhanced effectiveness. Future inquiries should encompass more rigorous studies to establish a more robust evidence foundation and facilitate progress in this domain.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19175"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of rhizosphere soil between three plantation types in Karst Rocky Desertification area by widely targeted metabolomics.","authors":"Tongtong Guo, Ninghua Zhu, Ziqian Pan, Peng Dang","doi":"10.7717/peerj.19131","DOIUrl":"10.7717/peerj.19131","url":null,"abstract":"<p><p>Understanding the differences in rhizosphere soil microbial metabolites between severely and mildly rocky desertified areas is crucial for developing ecological restoration strategies and land management measures in rocky desertification regions. This study systematically analyzed the differences in rhizosphere soil microbial metabolites of <i>Toona sinensis</i>, <i>Vernicia fordii</i>, and <i>Cornus wilsoniana</i> in severely and mildly rocky desertified areas of Western Hunan using untargeted metabolomics. The results showed that the types and quantities of primary and secondary metabolites in the rhizosphere soil of severely rocky desertified areas were significantly lower than those in mildly rocky desertified areas. Additionally, under severe rocky desertification conditions, 15 common compounds (<i>e.g.</i>, 17a-estradiol, adenine, all-trans-retinoic acid) were significantly increased in the rhizosphere soil microbial metabolites of the three tree species. These compounds may provide defense mechanisms for plants to adapt to harsh environments. KEGG metabolic pathway analysis revealed that under severe rocky desertification conditions, <i>Toona sinensis</i>, <i>Vernicia fordii</i>, and <i>Cornus wilsoniana</i> shared six enriched pathways, which play an important role in the biosynthesis of compounds such as phenylpropanoids and unsaturated fatty acids. By revealing the differences in rhizosphere soil microbial metabolites, this study not only deepens the understanding of rocky desertification ecosystems but also provides valuable scientific evidence for ecological restoration and sustainable land management.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19131"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis identified candidate genes associated with kernel row number in maize.","authors":"Shukai Wang, Yancui Wang, Xitong Xu, Dusheng Lu, Baokun Li, Yuxin Zhao, Senan Cheng, Zhenhong Li, Cuixia Chen","doi":"10.7717/peerj.19143","DOIUrl":"10.7717/peerj.19143","url":null,"abstract":"<p><p>Kernel row number (KRN) is a crucial trait in maize that has a high impact on yield. However, KRN is a typical quantitative trait with only a few genes being verified so far. Here, two maize inbred lines with contrasting KRN were used to perform transcriptome analysis at five early ear developmental stages. Pairwise differential gene expression analyses were performed, and a total of 11,897 line-specific differentially expressed genes (DEGs) were detected between the two lines across the five development stages. Clustering analysis of line-specific DEGs revealed that the trends of gene expression changed significantly in the five stages, thus the five stages were further divided into two development phases: Phase I (V6-V8) and Phase II (V9-V10). Gene ontology enrichment analysis revealed that different transcriptional pathways were activated in the two phases. DEGs in Phase I were significantly enriched in morphogenesis and differentiation processes and hormone regulation. Of the 5,850 line-specific DEGs in Phase I, 2,132 genes were in known quantitative trait loci (QTLs) or flanking regions of quantitative trait nucleotides (QTNs), of which 92 were repeatedly detected in QTLs where QTNs also exist. The 92 high-probability candidate genes included development-related transcription factors (SBP-box and AP2/EREBP TFs) as well as genes involved in hormone homeostasis and signaling. Our study provides genetic resources for the elucidation of the molecular mechanisms of KRN development and reference for the cloning of candidate genes.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19143"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed tomography-based radiomic features combined with clinical parameters for predicting post-infectious bronchiolitis obliterans in children with adenovirus pneumonia: a retrospective study.","authors":"Li Zhang, Ling He, Guangli Zhang, Xiaoyin Tian, Haoru Wang, Fang Wang, Xin Chen, Yinglan Zheng, Man Li, Yang Li, Zhengxiu Luo","doi":"10.7717/peerj.19145","DOIUrl":"10.7717/peerj.19145","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a model incorporating computed tomography (CT) radiomic features and clinical parameters for predicting bronchiolitis obliterans (BO) with adenovirus pneumonia in children.</p><p><strong>Methods: </strong>A total of 165 children with adenovirus pneumonia between October 2013 and February 2020 were enrolled retrospectively. Among them, BO occurred in 70 patients, and the remaining 95 patients did not have BO. These children were stratified into training and testing groups at a ratio of 7:3. Manual segmentation of lesions in baseline CT images during acute pneumonia was performed to extract radiomic features. Multiple statistical methods were used to determine the best radiomic features. Combined models based on radiomic and clinical features were established <i>via</i> logistic regression (LR), random forest (RF), and support vector machine (SVM) algorithms. Model performance was evaluated <i>via</i> the area under the receiver operating characteristic curve (AUC).</p><p><strong>Results: </strong>A total of 2,264 radiomic features were extracted from the lesions, from which 10 optimal radiomic features were ultimately selected. The length of hospitalization, number of pneumonia lobes, and optimal radiomic features were incorporated into the combined models. In the training group, the AUCs of the combined LR, RF and SVM models were 0.946, 0.977, and 0.971, respectively; while in the testing group, they yielded AUCs of 0.890, 0.859, and 0.885, respectively. The predictive performance of these combined models surpassed that of the radiomic and clinical models.</p><p><strong>Conclusion: </strong>Combining CT-based radiomic features with clinical parameters can offer an effective noninvasive model to predict BO in children with adenovirus pneumonia.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19145"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microecological recombination of <i>Angelica sinensis</i> driven by the transplanting of \"alpine seedling-cellar planting-dam cultivation\".","authors":"Dongmei He, Weiping Gao, Zhanling Zhang, Jinniu Xing, Guiqi Han, Hai Wang, Zhuyun Yan","doi":"10.7717/peerj.19208","DOIUrl":"10.7717/peerj.19208","url":null,"abstract":"<p><p>Transplanting is important for obtaining and maintaining excellent germplasm of cultivated plants. During plant transplantation, the endophytic microbial community regularly reorganizes, which may be crucial for plant germplasm rejuvenation. <i>Angelica sinensis</i>, a widely used medicinal and edible plant, relies on transplanting for its exceptional quality. To explore the microecological recombination of <i>A. sinensis</i> during the transplanting process of \"alpine seedling-cellar planting-dam cultivation\", this study analyzed shifts in endophytic and soil microbial communities across the three transplanting stages in Min County, Gansu Province, China. High-throughput sequencing revealed significant changes, with 82.27% to 84.65% of bacteria and 93.19% to 93.49% of fungi species altering in transplanted <i>Angelica</i>. Main findings indicate that Mortierellomycota, Actinobacteriota, and Myxococcota were dominant in cellar planting root and cellar rhizosphere soil, contrasting with Firmicutes predominance in alpine and dam areas. Notably, potentially pathogenic endophytes like <i>Fusarium</i> and <i>Xanthomonas</i> decreased post-alpine seedling and cellar planting, favoring a healthier plant environment. Cellar planting root exhibited a rich accumulation of psychrophilic flora, including <i>Tetracladium</i>, <i>Pseudomonas</i>, and <i>Flavobacterium</i>, alongside a unique dominance of <i>Mortierella</i> fungi. Microbial co-occurrence network analysis highlighted cellar planting root as pivotal, suggesting its importance in microbial interactions. In conclusion, transplanting significantly reshaped <i>A. sinensis</i>'s endophytic flora, with fungi showing more pronounced recombination than bacteria. Soil microbial communities emerged as crucial drivers of this recombination, facilitating the overwintering of <i>A. sinensis</i>, reducing diseases, and rejuvenating the germplasm. Transplanting-driven microecological reorganization is an important scientific mechanism for the high-quality production of cultivated medicinal plants.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19208"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory mechanisms of long-term volleyball practice on visuospatial working memory capacity: an fNIRS study.","authors":"Wen Zhang, Jingru Liu, Fangfang Hu, Yang Liu, Chao Kan","doi":"10.7717/peerj.19153","DOIUrl":"10.7717/peerj.19153","url":null,"abstract":"<p><strong>Objective: </strong>Visuospatial working memory capacity is crucial for daily life and various cognitive processes. Previous studies have shown that physical training not only improves physical fitness but also visual visuospatial working memory capacity. However, few studies have explored visuospatial working memory improvement and brain plasticity changes with long-term volleyball exercise. Therefore, the purpose of this study was to gain insight into whether there is a relationship between long-term volleyball practice and visuospatial working memory and the effects on the prefrontal lobes of the brain.</p><p><strong>Methods: </strong>Neural correlates of visuospatial working memory in elite (<i>n</i> = 23; raining age mean: 8.27 ± 1.75 years; age: 21.07 ± 1.58 years) and novice (<i>n</i> = 23; raining age mean: 1.81 ± 0.56 years; age: 20.53 ± 1.36 years) volleyball athletes are examined to uncover potential skill-based differences. Functional near-infrared spectroscopy (fNIRS) data from 46 participants performing visuospatial working memory test reveal compelling results.</p><p><strong>Results: </strong>Compared with the novice group, the experts showed a higher accuracy rate (ACC) (<i>p</i> = 0.021) and shorter reaction time (RT) (<i>p</i> = 0.019) in the visuospatial working memory test. fNIRS data showed increased oxygen activation in the right dorsolateral prefrontal lobe (<i>p</i> < 0.05) and the right frontal region (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Studies have shown that long-term volleyball training can significantly enhance individuals' visuospatial working memory capacity. This enhancement was mainly reflected in the fact that athletes who participated in long-term volleyball training demonstrated faster operational processing speed and higher accuracy in visuospatial working memory tasks, and plasticity changes in dorsolateral prefrontal and prefrontal pole regions. We also found no significant linear relationship between specific brain activation and behavioral performance in expert athletes.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19153"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New digital anatomical data of <i>Keichousaurus hui</i> (Reptilia: Sauropterygia) and its phylogenetic implication.","authors":"Jiayu Xu, Yu Guo, Yucong Ma, Wei Wang, Long Cheng, Fenglu Han","doi":"10.7717/peerj.19012","DOIUrl":"10.7717/peerj.19012","url":null,"abstract":"<p><p>Three complete skulls of <i>Keichousaurus hui</i> from the Middle Triassic Xingyi Fauna of southwestern China were examined using high-resolution computed tomography (CT) scanning. The CT images allow a few refinements and supplements in cranial anatomy. Some previously ambiguous anatomical characters were identified, including the presence of an L-shaped ectopterygoid that extends from the lateral side of the pterygoid and bends ventrally, the wedge-shaped posterolateral process of the frontal, the trapezoidal pterygoid for articulating with the palatine, and a rodlike basioccipital tuber that extends posterolaterally. These new features provide new detailed anatomical information for taxonomy. The new phylogenetic analysis of Sauropterygiformes places <i>Keichousaurus</i> as an eosauropterygian that is more basal than the monophyly which includes Nothosauridae and Pistosauroidea. Moreover, the result also suggests that <i>Keichousaurus</i> is more closely related to Chinese pachypleurosaurs-like eosauropterygians than to European pachypleurosaurs and more derived than other Chinese pachypleurosaurs-like forms.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19012"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis of the rhizosphere microbial communities among three species of the <i>Salix</i> genus.","authors":"Tianqing Feng, Juan Li, Xiaoning Mao, Xionglian Jin, Liang Cheng, Huichun Xie, Yonggui Ma","doi":"10.7717/peerj.19182","DOIUrl":"10.7717/peerj.19182","url":null,"abstract":"<p><p>Rhizosphere microorganisms exert a significant influence in counteracting diverse external stresses and facilitating plant nutrient uptake. While certain rhizosphere microorganisms associated with <i>Salix</i> species have been investigated, numerous rhizosphere microorganisms from various <i>Salix</i> species remain underexplored. In this study, we employed high-throughput sequencing to examine the rhizosphere bacterial and fungal communities composition and diversity of three <i>Salix</i> species: <i>Salix zangica</i> (SZ), <i>Salix myrtilllacea</i> (SM), and <i>Salix cheilophila</i> (SC). Furthermore, the BugBase and FUNGuild were utilized to predict the functional roles of bacterial and fungal microorganisms. The findings revealed notable variations in the alpha and beta diversities of bacterial and fungal communities among the three <i>Salix</i> species exhibited significant differences (<i>p</i> < 0.05). The relative abundance of <i>Flavobacterium</i> was highest in the SZ samples, while <i>Microvirga</i> exhibited significant enrichment in the SM samples. <i>Microvirga</i> and <i>Vishniacozyma</i> demonstrate the highest number of nodes within their respective bacterial and fungal community network structures. The functions of bacterial microorganisms, including Gram-positive, potentially pathogenic, Gram-negative, and stress-tolerant types, exhibited significant variation among the three <i>Salix</i> species (<i>p</i> < 0.05). Furthermore, for the function of fungal microbe, the ectomycorrhizal guild had the highest abundance of symbiotic modes. This results demonstrated the critical role of ectomycorrhizal fungi in enhancing nutrient absorption and metabolism during the growth of <i>Salix</i> plants. Additionally, this findings also suggested that <i>S. zangica</i> plant was better well-suited for cultivation in stressful environments. These findings guide future questions about plant-microbe interactions, greatly enhancing our understanding of microbial communities for the healthy development of <i>Salix</i> plants.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19182"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}