PeerJ最新文献

{"title":"Sex differences in absolute and relative changes in muscle size following resistance training in healthy adults: a systematic review with Bayesian meta-analysis.","authors":"Martin C Refalo, Greg Nuckols, Andrew J Galpin, Iain J Gallagher, D Lee Hamilton, Jackson J Fyfe","doi":"10.7717/peerj.19042","DOIUrl":"https://doi.org/10.7717/peerj.19042","url":null,"abstract":"<p><strong>Background: </strong>Muscle hypertrophy may be influenced by biological differences between males and females. This meta-analysis investigated <i>absolute</i> and <i>relative</i> changes in muscle size following resistance training (RT) between males and females and whether measures of muscle size, body region assessed, muscle fibre type, and RT experience moderate the results.</p><p><strong>Methods: </strong>Studies were included if male and female participants were healthy (18-45 years old) adults that completed the same RT intervention, and a measure of pre- to post-intervention changes in muscle size was included. Out of 2,720 screened studies, 29 studies were included in the statistical analysis. Bayesian methods were used to estimate a standardised mean difference (SMD), log response ratio (lnRR) with exponentiated percentage change (Exp. % Change of lnRR), and probability of direction (<i>pd</i>) for each outcome.</p><p><strong>Results: </strong>Absolute increases in muscle size slightly favoured males compared to females (SMD = 0.19 (95% HDI: 0.11 to 0.28); <i>pd</i> = 100%), however, relative increases in muscle size were similar between sexes (Exp. % Change of lnRR = 0.69% (95% HDI: -1.50% to 2.88%)). Outcomes were minimally influenced by the measure of muscle size and not influenced by RT experience of participants. Absolute hypertrophy of upper-body but not lower-body regions was favoured in males. Type I muscle fibre hypertrophy slightly favoured males, but Type II muscle fibre hypertrophy was similar between sexes.</p><p><strong>Conclusion: </strong>Our findings strengthen the understanding that females have a similar potential to induce muscle hypertrophy as males (particularly when considering relative increases in muscle size from baseline) and findings of our secondary analyses should inform future research that investigates sex differences in highly trained participants and muscle fibre type-specific hypertrophy.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19042"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational overweight decreased risk of antepartum hemorrhage in pregnant women with complete placenta previa: a retrospective study.","authors":"Jie Yang, Shaoxin Ye, Bihua Xuan, Zhengping Liu, Dazhi Fan","doi":"10.7717/peerj.19091","DOIUrl":"https://doi.org/10.7717/peerj.19091","url":null,"abstract":"<p><strong>Background: </strong>Antepartum hemorrhage (APH) is associated with perinatal mortality and maternal morbidity. Previous studies have reported that obesity in pregnancy adversely influences both fetal and neonatal outcomes. This study aimed to investigate gestational overweight and the risk of APH in pregnant women with complete placenta previa (CPP).</p><p><strong>Methods: </strong>This was a retrospective cohort study of pregnant women with CPP delivery at our hospital from 2013 to 2015. Outcomes were stratified according to APH and non-APH.</p><p><strong>Results: </strong>Of 193 pregnancies with CPP, 40.4% (78) were diagnosed with APH. Maternal weight and BMI at delivery were significantly decreased in women with APH (61.15 ± 8.73 <i>vs</i>. 65.22 ± 7.80, 24.47 ± 3.12 <i>vs</i>. 26.21 ± 2.85; <i>P</i> = 0.001, <i>P</i> = 0.001; respectively), and the prevalence of overweight at delivery was higher in the non-APH group compared to those in the APH group (54.9% (62) <i>vs</i>. 39.7% (27); OR 2.18; 95%CI [1.16-4.11]). After adjusting for gestation week and other potential confounding factors, maternal weight and BMI were associated with the APH (OR 0.95, 95%CI [0.91-0.99]; 0.85, 95%CI [0.75-0.97], respectively).</p><p><strong>Conclusion: </strong>Appropriate weight gain during pregnancy may decrease the risk of antepartum hemorrhage in pregnant women with complete placenta previa.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19091"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphofunctional changes in the immune system in colitis-associated colorectal cancer in tolerant and susceptible to hypoxia mice.","authors":"Dzhuliia Dzhalilova, Maria Silina, Anna Kosyreva, Nikolai Fokichev, Olga Makarova","doi":"10.7717/peerj.19024","DOIUrl":"https://doi.org/10.7717/peerj.19024","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;One of the effective strategies for the treatment of tumor diseases, including colitis-associated colorectal cancer (CAC), is immunotherapy. During inflammation, NF-κB is activated, which is connected with the hypoxia-inducible factor-HIF, regulating the immune cells functioning and influences the CAC development. Organisms differ according to their hypoxia resistance and HIF expression. Therefore, the aim of the study was to characterize the thymus, spleen and mesenteric lymph nodes morphofunctional features, as well as changes in the subpopulation composition of peripheral blood cells and mesenteric lymph nodes in tolerant and susceptible to hypoxia C57Bl/6 mice in CAC.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Hypoxia tolerance was assessed by gasping time measurement in hypobaric decompression chamber. Based on the outcome, the mice were assigned to three groups characterized as 'tolerant to hypoxia', 'normal', and 'susceptible to hypoxia'. A month after determining hypoxia resistance CAC was modeled by intraperitoneal azoxymethane (AOM) administration and three cycles of dextran sulfate sodium consumption. Mice were sacrificed on the 141st day after the AOM administration, a morphological, morphometric and immunohistochemical study of tumors, morphological and morphometric study of thymus and spleen, and subpopulation composition of peripheral blood cells and mesenteric lymph nodes assessment were carried out.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Tumors in tolerant and susceptible to hypoxia mice were represented by glandular intraepithelial neoplasia and adenocarcinomas, the area of which was larger in susceptible mice. Immunohistochemical study revealed a more pronounced Ki-67+ staining in tumors of susceptible mice. In CAC, only in tolerant mice, expansion of the thymic cortex was observed relative to the control group, while in susceptible ones, no changes were detected. Only in susceptible to hypoxia mice, spleen germinal centers of lymphoid follicles enlargement were observed. Only in susceptible mice during CAC, in comparison to the control group, the relative and absolute number of B-lymphocytes and relative-cytotoxic T-lymphocytes in blood increased. The relative cytotoxic T-lymphocytes and NK cells number in peripheral blood during CAC was higher in susceptible to hypoxia mice compared to tolerant ones. In susceptible to hypoxia mice, more pronounced changes in the mesenteric lymph nodes subpopulation composition of cells were revealed-only in them the absolute and relative number of B-lymphocytes and NK cells, the absolute number of cytotoxic T-lymphocytes increased, and the relative number of macrophages decreased.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Morphofunctional differences in the thymus, spleen, mesenteric lymph nodes and blood immune cells reactions indicated the more pronounced immune response to the CAC development in susceptible to hypoxia mice, which should be taken into account in experimental studies","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19024"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of cancer cell line-specific synergistic drug combinations based on multi-omics data.","authors":"Jiaqi Chen, Huirui Han, Lingxu Li, Zhengxin Chen, Xinying Liu, Tianyi Li, Xuefeng Wang, Qibin Wang, Ruijie Zhang, Dehua Feng, Lei Yu, Xia Li, Limei Wang, Bing Li, Jin Li","doi":"10.7717/peerj.19078","DOIUrl":"https://doi.org/10.7717/peerj.19078","url":null,"abstract":"<p><p>Compared to single-drug therapy, combination therapy involves the use of two or more drugs to reduce drug dosage, decrease drug toxicity, and improve treatment efficacy. We developed an extreme gradient boosting (XGBoost)-based drug-drug cell line prediction model (XDDC) to predict synergistic drug combinations. XDDC was based on XGBoost and used one of the largest drug combination datasets, NCI-ALMANAC. In XDDC, drug chemical structures, adverse drug reactions, and target information were selected as drug features; gene expression, methylation, mutations, copy number variations, and RNA interference data were used as cell line features; and pathway information was incorporated to link drug features and cell line features. XDDC improved the interpretability of drug combination features and outperformed other machine learning methods. It achieved an area under the curve (AUC) of 0.966 ± 0.002 and an AUPR of 0.957 ± 0.002 when cross-validated on NCI-ALMANAC data. Different types of omics data were evaluated and compared in the model. Literature and experimental verification confirmed some of our predictions. XDDC could help medical professionals to rapidly screen synergistic drug combinations against specific cancer cell lines.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19078"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of clinical deterioration within one year in chronic obstructive pulmonary disease using the systemic coagulation-inflammation index: a retrospective study employing multiple machine learning method.","authors":"Ling Hou, Ming Min, Rui Hou, Wei Tan, Minghua Zhang, Qianfei Liu","doi":"10.7717/peerj.18989","DOIUrl":"https://doi.org/10.7717/peerj.18989","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory response and the coagulation system are pivotal in the pathogenesis of clinical deterioration in chronic obstructive pulmonary disease (COPD), prompting us to hypothesize that the systemic coagulation-inflammation (SCI) index is associated with clinical deterioration in COPD.</p><p><strong>Methods: </strong>A cohort of 957 COPD patients (mean age: 68.4 ± 7.8 years; 74.4% male) from January 2018 to December 2021 was analyzed. Six machine learning models (XGBoost, logistic regression, Random Forest, elastic net (ENT), support vector machine (SVM), and K-nearest neighbors (KNN)) were evaluated using accuracy, precision, recall, F1-score, and the area under the receiver operating characteristic curve (AUC-ROC).</p><p><strong>Results: </strong>Our study encompassed 957 patients, out of which 171 were classified in the clinical deterioration of COPD (cd-COPD) cohort. Significant disparities in age, comorbidities like respiratory failure, C-reactive protein, lymphocyte count, red blood cell distribution width (RDW), SCI, procalcitonin (PCT), and D-dimer were depicted between the cd-COPD and non-cd-COPD groups. Concerning machine learning and model comparison, the SVM model showcased consistent performance and strong generalization capabilities on both the training and testing sets compared to the other five machine learning (ML) models. The SCI index, as the most influential predictor, demonstrated a median of 93.08 in cd-COPD compared to 81.67 in non-cd-COPD patients.</p><p><strong>Conclusion: </strong>The SCI is markedly elevated in cd-COPD patients compared to COPD patients, and SVM demonstrates reliable performance in cd-COPD prediction.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18989"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription of <i>Aspidosiphon</i> (<i>Paraspidosiphon</i>) <i>steenstrupii</i> Diesing, 1859 (Sipuncula: Aspidosiphonidae) and the reinstatement of three species.","authors":"Itzahi Silva-Morales, Luis F Carrera-Parra","doi":"10.7717/peerj.19003","DOIUrl":"https://doi.org/10.7717/peerj.19003","url":null,"abstract":"<p><p>Sipuncula, specifically the family Aspidosiphonidae, faces taxonomic challenges due to brief original descriptions and the poor condition or loss of the type material. Detailed standardized redescriptions are essential to understanding the diversification in this group. Herein, a comprehensive redescription of <i>Aspidosiphon</i> (<i>Paraspidosiphon</i>) <i>steenstrupii</i> based on an extensive material collection from the tropical Western Atlantic is provided. Based on morphological data and the analysis of COI sequences, we delimited <i>A</i>. (<i>P</i>.) <i>steenstrupii</i> morphologically, restricting its distribution to the tropical Western Atlantic. Also, the redescriptions and proposals for reinstatement of <i>A.</i> (<i>P.</i>) <i>exostomum</i>, <i>A</i>. (<i>P</i>.) <i>ochrus</i>, and <i>A</i>. (<i>P</i>.) <i>speculator</i>, previously considered junior synonyms of <i>A</i>. (<i>P</i>.) <i>steenstrupii</i>, are included. Furthermore, a comprehensive discussion on diagnostic morphological features to recognize aspidosiphonid species and a detailed revision of synonyms of <i>A</i>. (<i>P</i>.) <i>steenstrupii</i> are included. Notable differences in morphology and genetic data suggest the need for revising the taxonomic status of several synonyms within the family, highlighting underestimated diversity in sipunculans.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19003"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of biopsies of gastric cancer, intestinal and diffuse, and non-atrophic gastritis: an overview of loss of heterozygosity in Mexican patients.","authors":"Violeta Larios-Serrato, Hilda A Valdez-Salazar, Javier Torres, Margarita Camorlinga, Patricia Piña-Sánchez, Fernando Minauro, Martha-Eugenia Ruiz-Tachiquín","doi":"10.7717/peerj.18928","DOIUrl":"https://doi.org/10.7717/peerj.18928","url":null,"abstract":"<p><p>This study analyzed the loss of heterozygosity (LOH) effect on gastric cancer (GC) tumor samples from 21 Mexican patients, including diffuse (DGC) and intestinal (IGC) subtypes, as well as non-atrophic gastritis (NAG, control). Whole-genome high-density arrays were performed, and LOH regions were identified among the tissue samples. The differences in affected chromosomes were established among groups, with chromosomes 6 and 8 primarily affected in DGC and chromosomes 3, 16, and 17 in IGC. Functional pathway analysis revealed involvement in cancer-associated processes, such as signal transduction, immune response, and cellular metabolism. Five LOH-genes (IRAK1, IKBKG, PAK3, TKTL1, PRPS1) shared between GC and NAG suggest an early role in carcinogenesis. Specific genes were highlighted for Hallmarks of Cancer NAG-related genes (PTPRJ and NDUFS) were linked to cell proliferation and growth; IGC genes (GNAI2, RHOA, MAPKAPK3, MST1R) to genomic instability, metastasis, and arrest of cell death; and DGC genes to energy metabolism and immune evasion. These findings emphasize the role of LOH in GC pathogenesis and underscore the need for further research to understand LOH-affected genes and their diagnostic or evolution potential in cancer management. Portions of this text were previously published as part of a preprint (https://www.medrxiv.org/content/10.1101/2024.07.29.24311063v1).</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18928"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A first trial to supplement local hardwood sawdust at the first soaking for the cultivation of Shiitake (<i>Lentinula edodes</i>).","authors":"Stephanie Nabhan, Soukayna Haidar Ahmad, Zeina El Sebaaly, Teodor Nedelin, Youssef Najib Sassine","doi":"10.7717/peerj.18622","DOIUrl":"10.7717/peerj.18622","url":null,"abstract":"<p><p>Supplementation of the growing substrate has been reported to enhance the production of cultivated mushrooms; however, supplementation using nano-sized additives is not yet investigated on Shiitake (<i>Lentinula edodes</i>). The study investigated the potential of a nano-supplement (Lithovit®-Amino25) containing an admixture of 25% L-amino acids on shiitake cultivated on sawdust from locally available oak, maple, and apple trees (oak sawdust: OS, maple sawdust: MS, and apple sawdust: AS). Initially, sawdusts were enriched equally with wheat bran (WB) and at the first soaking, Lithovit®-Amino25 was applied at 5 g/L. Treatments were: T1: OS-WB (control), T2: OS-WB+ nano-amino, T3: MS-WB, T4: MS-WB+ nano-amino, T5: AS-WB, and T6: AS-WB+ nano-amino. Among non-supplemented substrates, complete mycelia run, fruiting, and harvest dates were faster in T1 than in T3 and T5. Complete mycelial development was delayed by 7, 5, 9, and 6 d in T3, T4, T5, and T6 compared to T1. The harvest date was delayed by 7.7-8.3 d on maple sawdust and by 10.5-12.7 d on apple sawdust compared to oak sawdust. However, nano-supplementation hastened fruiting and harvest dates (by 9.3 d) in T4 compared to T3. The biological yield of the second harvest was higher on supplemented than on non-supplemented maple and apple sawdusts. Only T1 and T2 showed consistency in production over two consecutive harvests. Nano-supplementation improved the total biological yield in T2, T4, and T6 by 9.8, 21.0, and 22.5%, respectively. Nevertheless, all treatments, except T4, had lower biological efficiencies compared with T1. In T4, results of stepwise regression showed a strong positive correlation (R² = 0.96) between the total biological yield and mushroom weight at the second harvest. Supplementation caused a slight or significant improvement in pileus diameter and mushroom firmness and a significant improvement in mushroom's crude protein (by 2.9-8.2% compared to T1) and fiber contents (by 1-2.3% compared to T1). In conclusion, supplemented maple sawdust would alternate oak sawdust for shiitake production, though other timings of supplementation might be further investigated to optimize production on this substrate.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18622"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injecting structure-aware insights for the learning of RNA sequence representations to identify m6A modification sites.","authors":"Yue Yu, Shuang Xiang, Minghao Wu","doi":"10.7717/peerj.18878","DOIUrl":"10.7717/peerj.18878","url":null,"abstract":"<p><p>N6-methyladenosine (m6A) represents one of the most prevalent methylation modifications in eukaryotes and it is crucial to accurately identify its modification sites on RNA sequences. Traditional machine learning based approaches to m6A modification site identification primarily focus on RNA sequence data but often incorporate additional biological domain knowledge and rely on manually crafted features. These methods typically overlook the structural insights inherent in RNA sequences. To address this limitation, we propose M6A-SAI, an advanced predictor for RNA m6A modifications. M6A-SAI leverages a transformer-based deep learning framework to integrate structure-aware insights into sequence representation learning, thereby enhancing the precision of m6A modification site identification. The core innovation of M6A-SAI lies in its ability to incorporate structural information through a multi-step process: initially, the model utilizes a Transformer encoder to learn RNA sequence representations. It then constructs a similarity graph based on Manhattan distance to capture sequence correlations. To address the limitations of the smooth similarity graph, M6A-SAI integrates a structure-aware optimization block, which refines the graph by defining anchor sets and generating an awareness graph through PageRank. Following this, M6A-SAI employs a self-correlation fusion graph convolution framework to merge information from both the similarity and awareness graphs, thus producing enriched sequence representations. Finally, a support vector machine is utilized for classifying these representations. Experimental results validate that M6A-SAI substantially improves the recognition of m6A modification sites by incorporating structure-aware insights, demonstrating its efficacy as a robust method for identifying RNA m6A modification sites.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18878"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Kaempferia chonburiensis</i> (Zingiberaceae), a new species from Thailand based on morphological and molecular evidence.","authors":"Pornpimon Wongsuwan, Boonmee Phokham, Pantamith Rattanakrajang, Chayan Picheansoonthon, Suchada Sukrong","doi":"10.7717/peerj.18948","DOIUrl":"10.7717/peerj.18948","url":null,"abstract":"<p><strong>Background: </strong><i>Kaempferia</i> is a genus belonging to the ginger family. Currently, this genus is comprised of about 63 species, mainly distributed from India to Southeast Asia. During our fieldwork, a new species of <i>Kaempferia</i> was found in Chon Buri Province, Thailand. The objective of this article was to provide morphological evidence and confirm its relationships in <i>Kaempferia</i> through molecular phylogenetic analysis.</p><p><strong>Methods: </strong>Plant samples were collected from field sites and investigated by conventional taxonomy and molecular techniques. The phylogenetic trees were reconstructed using the maximum likelihood criterion and Bayesian inference. The morphological evolution was also examined to elaborate the relationships among representative <i>Kaempferia</i> taxa.</p><p><strong>Results: </strong><i>Kaempferia chonburiensis</i> from southeastern Thailand is described and illustrated based on morphological features and its taxonomic placement was confirmed by molecular phylogenetic analyses and morphological evolution. An identification key is provided for the new <i>Kaempferia</i> species occurring in Thailand.</p><p><strong>Conclusion: </strong><i>Kaempferia chonburiensis</i> is a new enumerated species of <i>Kaempferia</i> from Thailand.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18948"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}