Parasite最新文献_第9页

Repositioning of moxidectin: a promising approach in cutaneous leishmaniasis therapy. 莫西丁的重新定位：皮肤利什曼病治疗的一个有前途的方法。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-04 DOI: 10.1051/parasite/2025035

Lynn Al Samra, Mohamad El Nahas, Ilham Mneimneh, Sima Tokajian, Georges Nemer, Aia Sinno, Kelven Rahy, Sergio Thoumi, Zahraa Zibara, Ahmad El Khatib, Dalal Sabbagh, Jacques Guillot, Louna Karam, Lazo Ali, Ruqaya Baghdadi, Charbel Al Khoury

{"title":"Repositioning of moxidectin: a promising approach in cutaneous leishmaniasis therapy.","authors":"Lynn Al Samra, Mohamad El Nahas, Ilham Mneimneh, Sima Tokajian, Georges Nemer, Aia Sinno, Kelven Rahy, Sergio Thoumi, Zahraa Zibara, Ahmad El Khatib, Dalal Sabbagh, Jacques Guillot, Louna Karam, Lazo Ali, Ruqaya Baghdadi, Charbel Al Khoury","doi":"10.1051/parasite/2025035","DOIUrl":"10.1051/parasite/2025035","url":null,"abstract":"Cutaneous leishmaniasis presents a significant challenge to public health due to its diverse clinical manifestations, resistance development, and treatment-related adverse effects. Here, we examined the efficacy of ivermectin, moxidectin (MOX), afoxolaner, and permethrin against all stages of Leishmania tropica and THP-1 cells. We also assessed the potential for resistance acquisition after 15 rounds of artificial selection. To elucidate the mode of action of MOX, we employed RNA sequencing, molecular dynamics simulation, and chloride flux assays. Additionally, we evaluated the therapeutic index of MOX using the Galleria mellonella infection model. MOX demonstrated the highest selectivity index against leishmaniasis (promastigotes: 0.58 μM; amastigotes: 0.96 μM; host cells: 60.29 μM). Moreover, MOX exhibited the lowest resistance acquisition in both promastigotes and intracellular amastigotes after 15 rounds of artificial selection, with resistance ratios of 17.23 and 4.59, respectively. Post-exposure to MOX, differential gene expression profiles showed both stage-specific and stage-unspecific enrichment of gene families involved in crucial biological processes. Moreover, molecular dynamics simulations revealed a potential neutralizing effect of MOX on the chloride channel of L. tropica. Specifically, MOX binds to the selectivity filter, potentially disrupting the osmotic equilibrium and thereby killing the parasite. The in vivo introduction of MOX significantly inhibited the growth of L. tropica in G. mellonella larvae, resulting in decreased rates of mortality and melanization. These findings indicate that MOX is a promising candidate for the treatment of L. tropica infections, warranting further investigation and potential consideration for clinical use.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"42"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo. 北京动物园圈养野生动物十二指肠贾第鞭毛虫和隐孢子虫已知宿主范围的扩大。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-23 DOI: 10.1051/parasite/2024079

Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi

{"title":"Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo.","authors":"Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi","doi":"10.1051/parasite/2024079","DOIUrl":"10.1051/parasite/2024079","url":null,"abstract":"Giardia duodenalis and Cryptosporidium spp. are significant zoonotic parasites that cause diarrhea and affect a diverse range of hosts. This study aimed to investigate the infection rates of these parasites in captive wildlife at Beijing Zoo. A total of 445 fecal samples were collected and analyzed using PCR. The infection rate of G. duodenalis was 3.82% (17/445), with assemblage A (n = 1), assemblage B (n = 13), and assemblage E (n = 3) identified. The infection rate of Cryptosporidium spp. was 0.22% (1/445), with only one instance of C. ryanae identified from cattle (Bos taurus). To the best of our knowledge, this study enhances the understanding of the host range of G. duodenalis in captive wildlife within China, highlighting infections in Corsac Fox (Vulpes corsac), Indian Rhinoceros (Rhinoceros unicornis), and Giraffe (Giraffa camelopardalis). These findings indicate that the infection rates of G. duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo are low, while showing that the known host range of G. duodenalis is expanding.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"4"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis. IgM三胞胎参与先天性弓形虫病诊断的蛋白质特征。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-11-26 DOI: 10.1051/parasite/2025065

Maria Carreno, Odile Villard, Isabelle Villena, Lucie Peyclit, Helene Diemer, Christine Schaeffer-Reiss, Coralie L'Ollivier

{"title":"Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis.","authors":"Maria Carreno, Odile Villard, Isabelle Villena, Lucie Peyclit, Helene Diemer, Christine Schaeffer-Reiss, Coralie L'Ollivier","doi":"10.1051/parasite/2025065","DOIUrl":"https://doi.org/10.1051/parasite/2025065","url":null,"abstract":"Congenital toxoplasmosis is a fetal infection resulting from the transplacental transmission of Toxoplasma gondii in mothers who seroconvert during pregnancy. Neonatal diagnosis has recently been improved through the identification by L'Ollivier et al. (2012) and Peyclit et al. (2023) of a pathognomonic marker for congenital toxoplasmosis: the IgM triplet, corresponding to three high molecular weight bands of 75, 90, and 100 kDa, respectively found on the mother-child immunoblot pair profile. This is a new concept, as these three IgM bands reflect an immune response targeting proteins involved in vertical transmission of T. gondii. These proteins may be T. gondii secreted or non-secreted effectors implicated in host cell invasion, immune modulation, and parasite virulence. In this study, immunoproteomic techniques allowed us to identify thirty-two relevant protein spots on immunoblot, including four specifically associated with the IgM triplet. Protein identification by LC-MS/MS revealed several T. gondii proteins as strong candidates for the IgM triplet. Each of these proteins is, directly or indirectly, involved in cellular invasion and may also play a role in transplacental transmission of T. gondii. Identifying these proteins opens several avenues of therapeutic research that could improve the management of congenital toxoplasmosis.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"72"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12656376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145637416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culturable bacteria and fungi in Ixodes, Dermacentor, Amblyomma and Ornithodoros ticks. 硬蜱、革蜱、弱视蜱和鸟蜱体内可培养的细菌和真菌。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-25 DOI: 10.1051/parasite/2025013

Marjorie Bruley, Célia Pasternicki, Noor Fattar, Julien Amoros, Maxime Duhayon, Karen McCoy, Olivier Duron

{"title":"Culturable bacteria and fungi in Ixodes, Dermacentor, Amblyomma and Ornithodoros ticks.","authors":"Marjorie Bruley, Célia Pasternicki, Noor Fattar, Julien Amoros, Maxime Duhayon, Karen McCoy, Olivier Duron","doi":"10.1051/parasite/2025013","DOIUrl":"10.1051/parasite/2025013","url":null,"abstract":"Ticks are ectoparasites harboring complex microbial communities, typically dominated by nutritional symbionts that produce B vitamins and sometimes including pathogens affecting human and animal health. However, ticks also host a variety of commensal microbes whose diversity remains poorly documented. In this study, we isolated and identified culturable bacteria and fungi associated with various tick species from the genera Ixodes, Dermacentor, Amblyomma, and Ornithodoros, collected from their natural habitats or hosts in France and French Guiana. A total of 111 bacterial and 27 fungal isolates were obtained which were then identified using both molecular and morphological approaches. Substantial fungal diversity was observed in a few ticks, whereas culturable bacteria displayed a broader distribution and diversity across tick species. Interestingly, the diversity of culturable bacteria and fungi revealed a microbiome structure that reflected the ecological niches of the tick host, indicating habitat-specific microbial associations and a potential ecological role in tick biology. The isolation of common gut bacteria of other arthropods, as well as the isolation of a viable entomopathogenic fungus, underscores the potential influence of these microbes on tick biology.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"20"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology of fatal Baylisascaris schroederi infection in a wild giant panda. 野生大熊猫施氏贝利蛔虫致死性感染的病理分析。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI: 10.1051/parasite/2025026

Lingling Chang, Danhui Zhang, Yashi Wang, Zun Ren, Yaping Wu, Qiang Zhang, Guanghui Zhao, Guanglin Pan, Xinglong Wang, Xiaomin Zhao, Dewen Tong

{"title":"Pathology of fatal Baylisascaris schroederi infection in a wild giant panda.","authors":"Lingling Chang, Danhui Zhang, Yashi Wang, Zun Ren, Yaping Wu, Qiang Zhang, Guanghui Zhao, Guanglin Pan, Xinglong Wang, Xiaomin Zhao, Dewen Tong","doi":"10.1051/parasite/2025026","DOIUrl":"10.1051/parasite/2025026","url":null,"abstract":"Baylisascaris schroederi McIntosh, 1939 (Ascarididae), a nematode specific to giant pandas (Ailuropoda melanoleuca), is a major health threat, particularly to wild populations. A 20-year-old wild adult female giant panda rescued from a Chinese nature reserve died with a 2-month history of emaciation and weakness. Necropsy was performed. Grossly, the giant panda was very thin with minimal fat stores throughout, and marked serous atrophy of fat around the kidneys. Mesenteric edema was very pronounced in the posterior intestine. The abdominal cavity contained approximately 5 L of orange-yellow, translucent, low-viscosity fluid. There were ca. 1,660 robust ascarids occupying the lumen of the esophagus, stomach, and intestines. Microscopically, the intestine showed moderate necrotizing and eosinophilic enteritis with adult nematodes, consistent with an ascarid. PCR and sequencing confirmed that the ascarid species was B. schroederi. This case highlights a fatal B. schroederi infection in a wild giant panda, with malnutrition and possible multiple organ failure identified as the primary causes of death.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"34"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and characterization of Cryptosporidium in sika deer from Northern China. 中国北方梅花鹿隐孢子虫的流行及特征。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI: 10.1051/parasite/2025029

Yan Tang, Nian-Yu Xue, Yang Gao, Zhen-Qiu Gao, Hong-Di Zhuang, Guang-Rong Bao, Hong-Wei Cao, Jing Liu, Jian-Ming Li, Shuo Liu

{"title":"Prevalence and characterization of Cryptosporidium in sika deer from Northern China.","authors":"Yan Tang, Nian-Yu Xue, Yang Gao, Zhen-Qiu Gao, Hong-Di Zhuang, Guang-Rong Bao, Hong-Wei Cao, Jing Liu, Jian-Ming Li, Shuo Liu","doi":"10.1051/parasite/2025029","DOIUrl":"10.1051/parasite/2025029","url":null,"abstract":"Cryptosporidium spp. are important zoonotic parasites that can cause moderate to severe diarrhea in humans and animals. However, the epidemiological data of Cryptosporidium in sika deer in China need to be updated. In this study, a total of 466 fecal samples were collected from sika deer in Shandong, Jilin, Liaoning, and Heilongjiang provinces. Nested PCR was used to amplify the SSU rRNA gene to detect Cryptosporidium spp. The results showed that the overall infection rate of Cryptosporidium spp. was 14.81%, with no significant differences among regions (p = 0.05). The highest infection rate was found in Heilongjiang Province (23.60%) and the lowest in Jilin Province (10.71%). The infection rate in summer (23.61%) seemed higher than that in autumn (13.20%), but the difference was not statistically significant (p = 0.30). Notably, young sika deer showed a significantly higher infection rate (28.21%) compared to adults (10.32%) (p < 0.0001). Sequence analysis identified two Cryptosporidium species/genotypes: Cryptosporidium deer genotype (98.55%) and Cryptosporidium ubiquitum (1.45%). Subtyping revealed that the C. ubiquitum isolate belonged to the zoonotic XIIa subtype. These findings provide new insights into the prevalence and genetic diversity of Cryptosporidium in sika deer and suggest that sika deer may act as a potential reservoir for zoonotic Cryptosporidium transmission.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"35"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioassay tests reveal for the first time pyrethroid resistance in Aedes mosquitoes from Franceville, southeast Gabon, Central Africa. 生物测定试验首次显示中非加蓬东南部Franceville的伊蚊对拟除虫菊酯类杀虫剂产生耐药性。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-01 DOI: 10.1051/parasite/2025036

Judicaël Obame-Nkoghe, Faël Moudoumi Kondji, El Hadji Diouf, Omar Thiaw, Brad Ghaven Niangui, Arnauld Ondo-Oyono, Yasmine Okomo-Nguema, Neil Michel Longo-Pendy, Franck Mounioko, Boris Makanga, Basile Kamgang, Christophe Paupy, Pierre Kengne, Patricks Voua Otomo, El Hadji Amadou Niang

{"title":"Bioassay tests reveal for the first time pyrethroid resistance in Aedes mosquitoes from Franceville, southeast Gabon, Central Africa.","authors":"Judicaël Obame-Nkoghe, Faël Moudoumi Kondji, El Hadji Diouf, Omar Thiaw, Brad Ghaven Niangui, Arnauld Ondo-Oyono, Yasmine Okomo-Nguema, Neil Michel Longo-Pendy, Franck Mounioko, Boris Makanga, Basile Kamgang, Christophe Paupy, Pierre Kengne, Patricks Voua Otomo, El Hadji Amadou Niang","doi":"10.1051/parasite/2025036","DOIUrl":"10.1051/parasite/2025036","url":null,"abstract":"The spread of resistance to insecticides, such as pyrethroids, in Aedes vectors increases the risk of spread of arboviral diseases. In Gabon, the insecticide resistance profiles of Ae. aegypti and Ae. albopictus species remain poorly known. During a study to monitor the dynamics of Aedes populations in Franceville, in south-east Gabon, the resistance profiles of these two species to pyrethroids, organophosphates and carbamates were assessed. Susceptibility tests on adults and synergist tests with piperonyl butoxide (PBO) were carried out as per the World Health Organization protocol. The results showed that Ae. aegypti and Ae. albopictus were susceptible to permethrin, pirimiphos-methyl and bendiocarb. However, both species were resistant to deltamethrin (mortality: 67% for Ae. aegypti; 33% for Ae. albopictus). Exposure to a 5-fold dose of deltamethrin increased mortality to 100% and 91% for Ae. aegypti and Ae. albopictus, respectively. Resistance to alpha-cypermethrin was also recorded (mortality: 82% for Ae. aegypti; 64.6% for Ae. albopictus). Pre-exposure to PBO resulted in the restoration of susceptibility to deltamethrin and alpha-cypermethrin for Ae. aegypti, and a significant increase in mortality for Ae. albopictus. These data provide the first evidence of pyrethroid resistance in Aedes in Gabon and could help to establish more effective control measures against arbovirus vectors.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"40"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Toxoplasma gondii from the placenta of northern fur seals (Callorhinus ursinus) and potential transplacental transmission of the parasite. 北毛海豹胎盘中刚地弓形虫的分离及其经胎盘传播的可能性。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-08-01 DOI: 10.1051/parasite/2025045

Gaohui Mao, Bingyan Guo, Shanshan Xie, Yurong Yang

{"title":"Isolation of Toxoplasma gondii from the placenta of northern fur seals (Callorhinus ursinus) and potential transplacental transmission of the parasite.","authors":"Gaohui Mao, Bingyan Guo, Shanshan Xie, Yurong Yang","doi":"10.1051/parasite/2025045","DOIUrl":"10.1051/parasite/2025045","url":null,"abstract":"Toxoplasma gondii infects almost all warm-blooded animals, including marine mammals. Toxoplasmosis has been reported in wild and captive marine mammals in North America; however, no viable T. gondii strains have been isolated from northern fur seals. In this study, reproduction and T. gondii infection status were investigated in 10 northern fur seals (Callorhinus ursinus), from tissues collected from 2012 to 2024 in China. Toxoplasma gondii infections were determined by the modified agglutination test (MAT), PCR, immunohistochemical (IHC) staining, and isolation of the parasite by bioassay in mice. MAT was performed using placenta or tissue exudates to detect anti-T. gondii IgG antibodies. Four of the 10 seals had anti-T. gondii antibodies; Toxoplasma gondii DNA was detected by PCR in placenta tissues of two of these four animals, and T. gondii antibody positive reactions were observed in four seals by IHC. A viable T. gondii strain, TgFurSealCHn1, was isolated from placenta of one seal by bioassay in mice. In all, five seals had signs of T. gondii infection, and three of them had fetal stillbirth. One stillborn fetus had T. gondii nucleic acid detected by PCR, indicating potential vertical transmission of the parasite. Multilocus genetic typing of the TgFurSealCHn1 isolate revealed ToxoDB #5 genotype, which had demonstrated avirulence in Swiss Webster outbred mice, and the ROP18/ROP5 type was 2/2. ToxoDB #5 is the dominant genotype of wild terrestrial and marine mammals in North America. This is the first report of a viable T. gondii strain isolated from northern fur seal placenta.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"48"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Irish cocktail of flatworm, earthworm and parasite DNAs: genomics of invasive land flatworms (Geoplanidae) reveal infestations by two new Mitosporidium species (Microsporidia). 扁虫、蚯蚓和寄生虫dna的爱尔兰鸡尾酒：入侵陆地扁虫（地扁虫科）的基因组学揭示了两种新的有丝孢子虫物种（微孢子虫）的侵扰。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-10-17 DOI: 10.1051/parasite/2025060

Romain Gastineau, Archie K Murchie, Leigh Winsor, Jean-Lou Justine

{"title":"An Irish cocktail of flatworm, earthworm and parasite DNAs: genomics of invasive land flatworms (Geoplanidae) reveal infestations by two new Mitosporidium species (Microsporidia).","authors":"Romain Gastineau, Archie K Murchie, Leigh Winsor, Jean-Lou Justine","doi":"10.1051/parasite/2025060","DOIUrl":"10.1051/parasite/2025060","url":null,"abstract":"According to the classical Enemy Release Hypothesis, one reason for the success of invasive species is that they have escaped their predators and parasites during migration to newly invaded territories. In this context, the discovery of any parasite of an invasive species is of particular interest. Here, we report the results of genomic investigations performed on two invasive species of land flatworms (Geoplanidae) collected in Northern Ireland, Kontikia andersoni Jones, 1981, and Australoplana sanguinea (Moseley, 1877). We describe the mitogenomes and paralogous RNA genes of both species. Prey DNA was detected in both flatworm species, providing molecular evidence that their diet includes earthworms. Unexpectedly, we detected sequences assigned to the microsporidian genus Mitosporidium Haag et al., 2015, which, prior to this study, included a single species. Each land flatworm species harboured its own species of Mitosporidium. For nomenclatural reasons, we could not assign binomial names to these species; instead, we designate them as Mitosporidium sp. JL467 (in K. andersoni) and Mitosporidium sp. JL472 (in A. sanguinea). For each new Mitosporidium species, we describe the gene content of the mitogenome and the complete cluster of nuclear ribosomal RNA genes. In the absence of direct evidence of host-parasite relationships, we discuss the possible hosts of these Microsporidia, which could be the flatworms themselves or their prey; the most likely hypothesis is that they are parasites of land flatworms. Other Mitosporidium species should be sought for in native land flatworms from the Australasian region, where the two invasive flatworm species originated. Investigations on the possible pathogenic role of these parasites are needed.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"67"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12534020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monogeneans on exotic Indian freshwater fish. 7. Results of a national study on ornamental fishes from 2019-2022. 外来的印度淡水鱼上的单系植物。7. 2019-2022年全国观赏鱼研究结果。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-05-12 DOI: 10.1051/parasite/2025021

Amit Tripathi, Chawan Matey, Kurt Buchmann, Christoph Hahn

{"title":"Monogeneans on exotic Indian freshwater fish. 7. Results of a national study on ornamental fishes from 2019-2022.","authors":"Amit Tripathi, Chawan Matey, Kurt Buchmann, Christoph Hahn","doi":"10.1051/parasite/2025021","DOIUrl":"10.1051/parasite/2025021","url":null,"abstract":"This study reports the results of a nationwide parasitological survey that was conducted from 2019 to 2022 to investigate the potential introduction of monogenean parasites into India via the ornamental fish trade. A total of 619 individual exotic ornamental fish representing 27 teleost species from nine families were collected from the country's major aquaria markets and examined for monogeneans. To identify monogeneans at the species level, we employed a morphometric analysis of sclerotised structures (haptoral and reproductive hard parts), as well as a molecular analysis of nuclear 28S rRNA and ITS2 regions. Indian conditions for importing exotic ornamental fish species require a pre-quarantine certificate, quarantine treatment, and post-quarantine follow-up. Despite these restrictions, 26 monogenean species from 12 known genera were detected and identified in 17 of the 27 fishes examined. Dactylogyrus was represented by a maximum of nine species, followed by Gyrodactylus with five. Cyprinidae was the most parasitised fish family (13 species), followed by Cichlidae (three species) and Helostomatidae, Poeciliidae, and Serrasalmidae (two species each). The majority of co-transported parasite species originated from Asia (65.38%, n = 17), followed by South America (23.07%, n = 6), North and Central America (7.69%, n = 2), and Africa (3.5%, n = 1). Three fish species were identified as the first host records for monogenean parasites: Chindongo socolofi for Cichlidogyrus tilapiae Paperna, 1960, Metynnis hypsauchen for Mymarothecium sp., and Betta splendens for Heteronchocleidus sp. In general, exotic populations had fewer parasite species than in their native distribution ranges.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"28"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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