Pathogens最新文献_第5页

Rabies Surveillance in Mainland Tanzania: A Scoping Review of Animal Rabies Occurrences (1993-2023). 坦桑尼亚大陆的狂犬病监测：1993-2023年动物狂犬病事件的范围审查。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-11 DOI: 10.3390/pathogens14090919

Emmanuel Kulwa Bunuma, Julius Keyyu, Joseph Maziku, Stella Bitanyi, Robert Fyumagwa, Katendi Changula, Benjamin Mubemba, Edgar Simulundu, Simbarashe Chitanga, Daniel L Horton, Abel Bulamu Ekiri, Hirofumi Sawa, Walter Muleya

{"title":"Rabies Surveillance in Mainland Tanzania: A Scoping Review of Animal Rabies Occurrences (1993-2023).","authors":"Emmanuel Kulwa Bunuma, Julius Keyyu, Joseph Maziku, Stella Bitanyi, Robert Fyumagwa, Katendi Changula, Benjamin Mubemba, Edgar Simulundu, Simbarashe Chitanga, Daniel L Horton, Abel Bulamu Ekiri, Hirofumi Sawa, Walter Muleya","doi":"10.3390/pathogens14090919","DOIUrl":"10.3390/pathogens14090919","url":null,"abstract":"Animal rabies remains underreported in low-income countries, hindering effective control. This scoping review aimed to map reported animal rabies cases, identify key reservoir species, and assess gaps in surveillance coverage in mainland Tanzania from 1993 to 2023. Specifically, it addressed the distribution of cases, species involved, and the extent of surveillance coverage during this period. Literature searches in PubMed, Google Scholar, and Science Direct were screened using Rayyan. Twenty articles published between 1993 and 2023 reported 7319 animal rabies cases across the Northern Zone (NZ), Southeastern Zone (SEZ), and Coastal Zone (CZ). In the NZ, domestic dogs accounted for most cases (5387), followed by jackals (225), cats (77), livestock (311), and various wildlife species including African wild dogs, bat-eared foxes, lions, cheetahs, and striped hyenas. Additionally, 102 cases involved unidentified animals. In SEZ, domestic dogs (588) were the primary source, followed by jackals (262), hyenas (8), cats (10), honey badgers (5), and leopards (2). In CZ, domestic dogs accounted for 94 cases. The findings confirm domestic dogs as the main rabies reservoir, highlighting the need for strengthened surveillance and control. The role of wildlife in rabies maintenance and spillover remains poorly understood and warrants further investigation, especially in enzootic hotspots.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E2 Tyrosine 102 Regulates MmuPV1 Pathogenesis In Vivo. E2酪氨酸102在体内调控MmuPV1发病机制

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-11 DOI: 10.3390/pathogens14090913

Jessica Gonzalez, Marsha DeSmet, Kennedy Stoll, Leny Jose, Neil Christensen, Elliot J Androphy

{"title":"E2 Tyrosine 102 Regulates MmuPV1 Pathogenesis In Vivo.","authors":"Jessica Gonzalez, Marsha DeSmet, Kennedy Stoll, Leny Jose, Neil Christensen, Elliot J Androphy","doi":"10.3390/pathogens14090913","DOIUrl":"10.3390/pathogens14090913","url":null,"abstract":"The papillomavirus (PV) life cycle is strictly controlled and can be divided into the following three distinct stages: initial infection, maintenance, and amplification. The papillomavirus E2 gene encodes a multifunctional protein responsible for regulating transcription and replication by recruiting viral and host factors to the viral DNA genome. Our lab has previously reported that tyrosine 102 may impact bovine (BPV) and human (HPV) viral replication in cell culture systems. This tyrosine is conserved in the E2 protein of the murine papillomavirus MmuPV1. To investigate how this amino acid impacts the MmuPV1 lifecycle in vivo, we generated potential phosphorylation mimetic (Y102E) and phosphorylation deficient (Y102F) mutants in the E2 open reading frame. The Y102F mutant protein supported both transcriptional activation and transient replication, while Y102E was defective. However, Y102E was capable of associating with E1 and the Brd4 C-terminal motif. When these E2-mutated MmuPV1 genomes were introduced into the skin of immunocompromised mice, only Y102F was capable of inducing papilloma development and production of infectious progeny virus. These findings demonstrate that while highly conserved, tyrosine at this position is not required by the virus. These data suggest that the chemical nature of the amino acid at this position can influence E2 activity and viral replication.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunization with mRNA-LNPs Encoding Ornithodoros Argasid Tick Antigens Induces Humoral Immune Responses and Tick Resistance. 编码银蛾蜱抗原的mRNA-LNPs免疫诱导体液免疫反应和蜱抗性。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-11 DOI: 10.3390/pathogens14090914

Ana Oleaga, Ana Laura Cano-Argüelles, María González-Sánchez, Rocío Vizcaíno-Marín, Ricardo Pérez-Sánchez

{"title":"Immunization with mRNA-LNPs Encoding Ornithodoros Argasid Tick Antigens Induces Humoral Immune Responses and Tick Resistance.","authors":"Ana Oleaga, Ana Laura Cano-Argüelles, María González-Sánchez, Rocío Vizcaíno-Marín, Ricardo Pérez-Sánchez","doi":"10.3390/pathogens14090914","DOIUrl":"10.3390/pathogens14090914","url":null,"abstract":"Argasid ticks Ornithodoros erraticus and Ornithodoros moubata are major vectors of zoonotic pathogens, including the African swine fever virus and relapsing fever Borrelia spp., and their control is essential to reduce disease transmission. In this study, we evaluated the immunogenicity and protective efficacy of four Ornithodoros tick antigens formulated as mRNA-lipid nanoparticles (mRNA-LNPs): OeSOD, OeTSP1, OmPLA2, and Om86. Rabbits were immunised with three doses of each mRNA-LNP construct, and immune responses and tick biological parameters were assessed following infestation with both tick species. All mRNA-LNP constructs induced antigen-specific IgG responses that recognised native proteins in tick saliva and midgut extracts. Vaccination resulted in significant reductions in female oviposition and fertility, which correlated with antibody levels, and yielded protective efficacies of 21.9-41.6% against O. moubata and 23.1-41.6% against O. erraticus. Notably, the mRNA-LNPs of OeSOD and OeTSP1 outperformed their recombinant counterparts against O. moubata, and Om86 mRNA-LNP conferred markedly improved protection against both O. moubata and O. erraticus. These findings highlight the potential of mRNA-LNP vaccines to induce effective anti-argasid tick immunity and provide a promising platform for the development of sustainable strategies to control argasid ticks and associated pathogens.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Giardin as an Immunomagnetic Enrichment Target for Multi-Host Detection of Giardia duodenalis Cysts. β-贾第鞭毛虫囊多宿主免疫富集靶点的研究

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-11 DOI: 10.3390/pathogens14090918

Hongyu Wang, Heng Yang, Chaofan Li, Mengge Chen, Xiaocen Wang, Xu Zhang, Pengtao Gong, Nan Zhang, Xichen Zhang, Jianhua Li, Xin Li

{"title":"β-Giardin as an Immunomagnetic Enrichment Target for Multi-Host Detection of Giardia duodenalis Cysts.","authors":"Hongyu Wang, Heng Yang, Chaofan Li, Mengge Chen, Xiaocen Wang, Xu Zhang, Pengtao Gong, Nan Zhang, Xichen Zhang, Jianhua Li, Xin Li","doi":"10.3390/pathogens14090918","DOIUrl":"10.3390/pathogens14090918","url":null,"abstract":"Giardiasis is a globally prevalent waterborne zoonosis. Rapid enrichment and detection technologies for this disease are essential. Cyst outer wall proteins are ideal targets for the enrichment and detection of cysts in the environment, but there are few available targets with suboptimal effectiveness. In this study, Giardia duodenalis (G. duodenalis) cysts were purified, and outer wall proteins were biotinylated, followed by streptavidin magnetic bead purification and mass spectrometry. Sixty-three novel cyst wall proteins were identified, and their functions were annotated through Gene Ontology (GO) and KEGG analyses. The β-giardin and α-1 giardin were among the newly identified and predicted to be located on the outer wall of G. duodenalis cysts. For the characterization of these two targets, we applied sequence analysis, prokaryotic expression, preparation of polyclonal antibodies, and determination of subcellular localization. Finally, based on β-giardin immunomagnetic beads were prepared using the polyclonal antibodies and tested for their enrichment efficiency. Immunomagnetic beads targeting β-giardin achieved 65% cyst enrichment efficiency in fecal samples, comparable to conventional methods. Clinical evaluation across 163 multi-host fecal samples (ferrets, Siberian tigers, red-crowned cranes) demonstrated concordance with nested PCR, successfully enriching cysts from PCR-positive specimens. The immunomagnetic beads method targeting β-giardin demonstrated effective G. duodenalis cyst enrichment in multi-host fecal samples. These results provide a proteomic framework for the cyst wall proteins of G. duodenalis, expanding the detection targets for G. duodenalis cysts. It also establishes a theoretical foundation for subsequent research on the composition and function of G. duodenalis cysts.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Signs at Admission: Prognostic Value of Initial Proinflammatory Markers for Sepsis and Mortality in ICU Patients. 入院时的第一体征：ICU患者脓毒症和死亡率的初始促炎标志物的预后价值。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090907

Fatma Aktaş, Yasemin Zer, Gülseren Elay, Mehmet Erinmez

{"title":"First Signs at Admission: Prognostic Value of Initial Proinflammatory Markers for Sepsis and Mortality in ICU Patients.","authors":"Fatma Aktaş, Yasemin Zer, Gülseren Elay, Mehmet Erinmez","doi":"10.3390/pathogens14090907","DOIUrl":"10.3390/pathogens14090907","url":null,"abstract":"Sepsis is a life-threatening condition and due to its non-specific symptoms, diagnosing sepsis and determining its severity remains difficult. Delays in recognizing sepsis can significantly increase mortality despite advances in antimicrobial therapy and resuscitation procedures. Biomarkers can help detect the presence and severity of sepsis, distinguish between types of infections, and evaluate treatment response. This prospective study aims to determine whether biomarker levels measured at the time of intensive care unit (ICU) admission can assist in the early prediction, diagnosis, and prognosis of sepsis and bacteremia. Blood samples were collected from 132 ICU patients upon admission and analyzed for CRP, IL-6, PCT, SAA, and endotoxin levels. Patients were monitored for sepsis development, blood culture results, and mortality. IL-6 levels demonstrated a significant association with prognosis and identified as an independent risk factor. CRP and PCT levels exhibit a significant effect on the development of sepsis in both univariate and multivariate analyses. Also, our study demonstrated that the presence of bacteremia in the initial blood sample taken from intensive care patients holds significant diagnostic and prognostic value even without waiting for species-level identification when combined with markers such as PCT, CRP, and IL-6.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Challenges in Salmonella Control: The Need for Innovative and Sustainable Disinfection Strategies in Poultry Farming. 沙门氏菌控制的新挑战：家禽养殖中需要创新和可持续的消毒策略。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090912

Nicla Gentile, Laura Lorenzo-Rebenaque, Ana Marco-Fuertes, Laura Montoro-Dasi, Clara Marin

{"title":"Emerging Challenges in Salmonella Control: The Need for Innovative and Sustainable Disinfection Strategies in Poultry Farming.","authors":"Nicla Gentile, Laura Lorenzo-Rebenaque, Ana Marco-Fuertes, Laura Montoro-Dasi, Clara Marin","doi":"10.3390/pathogens14090912","DOIUrl":"10.3390/pathogens14090912","url":null,"abstract":"Salmonella is one of the primary causes of foodborne infections worldwide and is often linked to the consumption of poultry products. Despite the implementation of numerous control programmes, the persistence of Salmonella in poultry environments remains a challenge, exacerbated by the emergence of strains resistant to traditional disinfectants. This review examines the key factors associated with the limitations of disinfection and the new strategies employed in poultry production, underscoring the need for more sustainable and effective alternative solutions. Various chemical (nanoparticles), physical (ultraviolet light, heat, pressurised steam, infrared radiation) and biological (bacteriophages, essential oils, and positive biofilm) treatments are examined. Of the various alternatives assessed, some have shown promising antimicrobial activity against Salmonella in vitro and under experimental conditions. However, their application in real-field settings is still limited, and few studies evaluate their effectiveness on a commercial scale. The review emphasises the importance of integrating these alternatives within broader biosecurity programmes, supported by clear regulations to minimise the risk of transmission. In conclusion, the adoption of innovative and sustainable approaches, combined with strengthened biosecurity measures, represents a key strategy to reduce Salmonella contamination in poultry farms, protect public health and promote responsible production systems.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium marinum Immune Evasion in Zebrafish. 斑马鱼的海洋分枝杆菌免疫逃逸。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090908

Priyank Kumar, Joshua Cameron, Beatrice Saviola, Vishwanath Venketaraman

{"title":"Mycobacterium marinum Immune Evasion in Zebrafish.","authors":"Priyank Kumar, Joshua Cameron, Beatrice Saviola, Vishwanath Venketaraman","doi":"10.3390/pathogens14090908","DOIUrl":"10.3390/pathogens14090908","url":null,"abstract":"Fish mycobacteriosis, a chronic progressive disease caused by nontuberculous mycobacteria (NTM), affects marine, brackish, and freshwater fish. Mycobacterium marinum (M. marinum), the most important of the NTM, infects fresh and marine water fish causing necrotizing granulomas and associated morbidity and mortality. M. marinum causes disease in zebrafish in a dose-dependent fashion. The M. marinum-induced disease in the zebrafish is associated with the development of necrotizing granulomas with abundant bacteria in the necrotic areas. Acute infection with high infectious doses of M. marinum infection in zebrafish was characterized by uncontrolled replication of the pathogen and death of all fish within 16 days, while chronic infections were marked by the formation of granulomas in different organs and longer survival in the range of 4-8 weeks. This review therefore synthesizes recent advances in our understanding of M. marinum's infection of zebrafish, molecular pathogenesis, virulence mechanisms, and immune evasion strategies in zebrafish, while also highlighting the host immune effector responses and the virulence mechanisms of M. marinum.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Surface Modification of a Dental Composite on the Adhesion of Streptococcus mitis, Streptococcus mutans, and Candida albicans: An In Vitro Study. 口腔复合材料表面改性对唇形链球菌、变形链球菌和白色念珠菌黏附的影响：体外研究

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090909

Santiago Arango-Santander, Claudia María Bedoya-Correa, Camila Soto, Santiago Bustamante, John Franco

{"title":"Effect of Surface Modification of a Dental Composite on the Adhesion of Streptococcus mitis, Streptococcus mutans, and Candida albicans: An In Vitro Study.","authors":"Santiago Arango-Santander, Claudia María Bedoya-Correa, Camila Soto, Santiago Bustamante, John Franco","doi":"10.3390/pathogens14090909","DOIUrl":"10.3390/pathogens14090909","url":null,"abstract":"Adhesion of different microorganisms to the surface of dental materials has generated significant interest since one of the most important requirements of biomaterials to be considered successful is their ability to withstand the damage caused by microorganisms that may lead to failure and the onset of different pathologies, such as caries. In vitro testing has demonstrated that surface modification is an alternative approach to reduce the adhesion of microorganisms to surfaces. The objective of this work was to assess the adhesion of Streptococcus mitis, Streptococcus mutans, and Candida albicans to a dental composite surface modified following a biomimetic approach and coated with salivary proteins. Soft lithography was used to copy the topography from the Crocosmia aurea leaf and then transfer it to the surface of dental composite discs that were coated with saliva proteins. Surfaces were characterized by contact angle and atomic force microscopy. S. mitis, S. mutans, and C. albicans were used to assess bacterial and fungal adhesion in monoculture and co-culture. The topographic modification of the surface of a dental composite reduced the adhesion of assessed microorganisms and the adhesion of these species in monoculture and co-culture on saliva-coated surfaces was higher than on topographically modified surfaces.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balancing Host Defense and Viral Tolerance for the Development of Next-Generation Broad-Spectrum Antiviral Agents. 平衡宿主防御和病毒耐受性以开发下一代广谱抗病毒药物。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090911

Xiujuan Zhao, Ruikun Du, Lijun Rong

引用次数: 0

Establishment of a Pseudovirus-Based Golden Hamster Model for the Attachment and Entry Stages of Hendra Virus Infection and Evaluation of Protective Immunity. 伪病毒金仓鼠亨德拉病毒附着和进入阶段模型的建立及保护性免疫评价。

IF 3.3 3区医学

Pathogens Pub Date : 2025-09-10 DOI: 10.3390/pathogens14090910

Tao Li, Binfan Liao, Danfeng Li, Jie Zhang, Chunhui Zhao, Yunfei Pei, Liping Chen, Meng Wang, Yawen Liu, Xi Wu, Weijin Huang, Jianhui Nie

{"title":"Establishment of a Pseudovirus-Based Golden Hamster Model for the Attachment and Entry Stages of Hendra Virus Infection and Evaluation of Protective Immunity.","authors":"Tao Li, Binfan Liao, Danfeng Li, Jie Zhang, Chunhui Zhao, Yunfei Pei, Liping Chen, Meng Wang, Yawen Liu, Xi Wu, Weijin Huang, Jianhui Nie","doi":"10.3390/pathogens14090910","DOIUrl":"10.3390/pathogens14090910","url":null,"abstract":"Objective: Establish an in vivo evaluation model focused on the attachment and entry stages of Hendra virus infection for protective immunity assessment.Methods: A golden hamster infection model based on recombinant Hendra-F/G pseudovirus was developed, and a luciferase luminescence assay was used to assess the optimal pseudoviral challenge in terms of route of infection, dose and detection time. The biodistribution of the pseudovirus in infected organs was evaluated using the IVIS spectral CT system. The protective effect of antibody prophylaxis was evaluated by measuring the luminescence intensity of pseudoviruses.Results: Intraperitoneal injection was identified as the optimal route of infection, and the optimal time of detection was 6 h post-challenge. Our model simulates the infection of the brain and lungs by live viruses, with the strongest infection occurring in the abdomen, especially in the intestinal organs. The dose of pseudovirus was linearly correlated with luminescence intensity. The infection model was able to differentiate the protective effect of monoclonal antibodies, with complete protection in the high-dose group.Conclusions: The recombinant Hendra-F/G pseudovirus hamster model allows the effective evaluation of prophylactic monoclonal antibodies, providing a crucial tool for studying Hendra virus infection and control strategies.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0