Pathogens最新文献_第5页

Antiviral Activity of Angelica Tenuissima Nakai against Influenza A Virus 中井当归对甲型流感病毒的抗病毒活性

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-04 DOI: 10.3390/pathogens13090761

Won-Jong Park, Gi-Sang Bae, Youn-Ho Han

{"title":"Antiviral Activity of Angelica Tenuissima Nakai against Influenza A Virus","authors":"Won-Jong Park, Gi-Sang Bae, Youn-Ho Han","doi":"10.3390/pathogens13090761","DOIUrl":"https://doi.org/10.3390/pathogens13090761","url":null,"abstract":"The influenza A virus poses a serious threat to human health and is an important global public health issue. The drugs currently used for treatment are becoming increasingly ineffective against influenza A viruses and require the development of new antiviral drugs. Angelica tenuissima Nakai (ATN), a traditional herbal medicine belonging to the Umbelliferae family, exhibits a broad range of pharmacological activities, including inflammation, headache, and cold symptoms. In the present study, based on target protein identification, functional enrichment analysis, and gene set comparisons, we first suggested that ATN has potential therapeutic effects against influenza A virus infection. Next, methylthiazol tetrazolium (MTT) and sulforhodamine B colorimetric (SRB) assay results revealed that ATN exhibited low cytotoxicity in Madin–Darby canine kidney (MDCK) cells. The antiviral properties of ATN were observed against H1N1 and H3N2 virus strains. Microscopy confirmed the increased survival rate of the host cells. Further time-of-addition experiments revealed that the addition of ATN before virus adsorption showed similar results to the whole period of treatment. The pre- and co-treated groups showed lower levels of viral RNA (M1 protein). The results of this study suggest that ATN exhibits antiviral properties against the influenza A virus. These therapeutic properties of ATN can serve as a theoretical basis for further research on the applicability of ATN in the development of antiviral agents.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Climate on Human Dengue Infections in the Caribbean 气候对加勒比地区人类登革热感染的影响

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-03 DOI: 10.3390/pathogens13090756

Kirk Osmond Douglas, Karl Payne, Gilberto Sabino-Santos, Peter Chami, Troy Lorde

{"title":"The Impact of Climate on Human Dengue Infections in the Caribbean","authors":"Kirk Osmond Douglas, Karl Payne, Gilberto Sabino-Santos, Peter Chami, Troy Lorde","doi":"10.3390/pathogens13090756","DOIUrl":"https://doi.org/10.3390/pathogens13090756","url":null,"abstract":"Climate change is no longer a hypothetical problem in the Caribbean but a new reality to which regional public health systems must adapt. One of its significant impacts is the increased transmission of infectious diseases, such as dengue fever, which is endemic in the region, and the presence of the Aedes aegypti mosquito vector responsible for transmitting the disease. (1) Methods: To assess the association between climatic factors and human dengue virus infections in the Caribbean, we conducted a systematic review of published studies on MEDLINE and Web of Science databases according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. (2) Results: In total, 153 papers were identified, with 27 studies selected that met the inclusion criteria ranging from the northern and southern Caribbean. Rainfall/precipitation and vapor pressure had a strong positive association with dengue incidence, whereas the evidence for the impact of temperatures was mixed. (3) Conclusions: The interaction between climate and human dengue disease in the Caribbean is complex and influenced by multiple factors, including waste management, infrastructure risks, land use changes, and challenged public health systems. Thus, more detailed research is necessary to understand the complexity of dengue within the wider Caribbean and achieve better dengue disease management.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse Head-to-Tail Sequences in the Circular Genome of Human Bocavirus Genotype 1 among Children with Acute Respiratory Infections Implied the Switch of Template Chain in the Rolling-Circle Replication Model 急性呼吸道感染儿童中人博卡病毒基因1型环状基因组中不同的头尾序列意味着滚圆复制模型中模板链的转换

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-03 DOI: 10.3390/pathogens13090757

Kexiang Zhang, Ri De, Yanpeng Xu, Zhenzhi Han, Runan Zhu, Yu Sun, Liping Jia, Dongmei Chen, Yutong Zhou, Qi Guo, Yao Yao, Shuang Liu, Dong Qu, Yuan Qian, Linqing Zhao

{"title":"Diverse Head-to-Tail Sequences in the Circular Genome of Human Bocavirus Genotype 1 among Children with Acute Respiratory Infections Implied the Switch of Template Chain in the Rolling-Circle Replication Model","authors":"Kexiang Zhang, Ri De, Yanpeng Xu, Zhenzhi Han, Runan Zhu, Yu Sun, Liping Jia, Dongmei Chen, Yutong Zhou, Qi Guo, Yao Yao, Shuang Liu, Dong Qu, Yuan Qian, Linqing Zhao","doi":"10.3390/pathogens13090757","DOIUrl":"https://doi.org/10.3390/pathogens13090757","url":null,"abstract":"Head-to-tail sequences have been reported in human bocavirus (HBoV) 1-4. To reveal their features and functions, HBoV DNA was screened among respiratory specimens from pediatric patients with an acute respiratory infection (ARI) between April 2020 and December 2022, followed by HBoV genotyping. Head-to-tail sequences were detected using nested PCR, TA cloning, and Sanger sequencing, and these findings were confirmed by mNGS and amplicon sequencing. The secondary structure was predicted using the Mfold web server. The results indicated that head-to-tail sequences were detected in 42 specimens through TA cloning from 351 specimens positive for HBoV1 DNA, yielding 92 sequences into 32 types and 2 categories. Additionally, head-to-tail sequences were detected in 16 specimens by amplicon sequencing, yielding 60 sequences categorized into 23 types. The 374nt type, detected in 13 specimens, contains variants 374a and 374b, which differ in the unpaired loop regions of the palindrome or complementary reverse sequences, implying a switch of template chains during the replication process. The mNGS results in three specimens confirmed the presence of circular genome in copies below 1%. In conclusion, head-to-tail sequences of HBoV1 were common in children with ARI and were highly diverse in length and sequences. The variants may be generated by the switch of the template chain in the rolling-circle replication model.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influenza B Virus Vaccine Innovation through Computational Design 通过计算设计创新乙型流感病毒疫苗

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-02 DOI: 10.3390/pathogens13090755

Matthew J. Pekarek, Eric A. Weaver

引用次数: 0

Serological Assessment of Lyme borreliosis in Bulgaria: A Nationwide Study 保加利亚莱姆包虫病血清学评估：一项全国性研究

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-02 DOI: 10.3390/pathogens13090754

Kim Ngoc, Iva Trifonova, Teodora Gladnishka, Evgenia Taseva, Elitsa Panayotova, Iva Vladimirova, Vladislava Ivanova, Eleonora Kuteva, Iva Christova

{"title":"Serological Assessment of Lyme borreliosis in Bulgaria: A Nationwide Study","authors":"Kim Ngoc, Iva Trifonova, Teodora Gladnishka, Evgenia Taseva, Elitsa Panayotova, Iva Vladimirova, Vladislava Ivanova, Eleonora Kuteva, Iva Christova","doi":"10.3390/pathogens13090754","DOIUrl":"https://doi.org/10.3390/pathogens13090754","url":null,"abstract":"Lyme borreliosis (LB), a tick-borne infection caused by bacteria in the Borrelia burgdorferi sensu lato complex, is increasingly prevalent on the Balkan Peninsula, including Bulgaria, where it is the most common tick-borne disease. This study aimed to assess the seroprevalence of LB across Bulgaria by analyzing 1892 serum samples for specific IgG antibodies using a two-tier testing protocol involving an ELISA and immunoblot methods. The results revealed an overall seroprevalence rate of 5.4%, with significant variation based on age, sex, and residence. Seroprevalence increased with age, peaking at 8.4% in individuals over 65 years. Males had a seroprevalence of 8.4% compared to 3.3% in females, and rural residents showed higher seroprevalence (10.2%) compared to urban residents (4.4%). Regional analysis indicated that seroprevalence ranged from 0.0% to 20.0%, with higher rates in northern provinces such as Gabrovo (18.9%) and Targovishte (20.0%). This study highlights the importance of two-step testing protocols for accurate diagnosis and underscores the need for increased awareness and further research to enhance public health measures and the management of LB in Bulgaria.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hantavirus Pulmonary Syndrome Outbreak Anticipation by a Rapid Synchronous Increase in Rodent Abundance in the Northwestern Argentina Endemic Region: Towards an Early Warning System for Disease Based on Climate and Rodent Surveillance Data 通过阿根廷西北部流行地区啮齿动物数量的快速同步增长预测汉坦病毒肺综合征的爆发：基于气候和啮齿动物监测数据建立疾病预警系统

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-02 DOI: 10.3390/pathogens13090753

Ignacio Ferro, Walter Lopez, Flavia Cassinelli, Sara Aguirre, Griet A. E. Cuyckens, Sebastián Kehl, Daira Abán-Moreyra, Paola Castillo, Carla Bellomo, José Gil, Valeria P. Martinez

{"title":"Hantavirus Pulmonary Syndrome Outbreak Anticipation by a Rapid Synchronous Increase in Rodent Abundance in the Northwestern Argentina Endemic Region: Towards an Early Warning System for Disease Based on Climate and Rodent Surveillance Data","authors":"Ignacio Ferro, Walter Lopez, Flavia Cassinelli, Sara Aguirre, Griet A. E. Cuyckens, Sebastián Kehl, Daira Abán-Moreyra, Paola Castillo, Carla Bellomo, José Gil, Valeria P. Martinez","doi":"10.3390/pathogens13090753","DOIUrl":"https://doi.org/10.3390/pathogens13090753","url":null,"abstract":"Hantavirus pulmonary syndrome (HPS) is an American emerging disease caused by the rodent-borne virus genus Orthohantavirus (Family: Hantaviridae: Order: Elliovirales Class: Bunyaviricetes). In Argentina, almost half of the HPS infections occur in the northwestern endemic region. In this study, we monitored rodent abundance during 2022 and 2023 in three sites with different sampling methods (removal trapping, live trapping and hunted rodents by domestic cats) to evaluate their relationship with human infections. We found a similar pattern of variation in rodent abundance across time, and particularly a synchronous rise of rodent abundance that anticipated an HPS outbreak in 2023. Our dynamic regression models revealed a positive relationship between HPS cases and rodent abundance with a three-month lag, as well as rainfall with an eight-month lag. Our results provide a framework for the planning and implementation of public health prevention campaigns based on climatology and rodent monitoring. Domestic cats bringing rodents into houses can be an overlooked risk factor, particularly if viral shedding of infected rodents is magnified by stress. HPS is a disease of public health concern due to its high mortality rate, the lack of a specific therapeutic treatment and no vaccine. Thus, prevention of infections is of the utmost importance.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Trichoderma spp.: A Promising Approach to Control Apple Scab Disease 利用毛霉菌属（Trichoderma spp：控制苹果疮痂病的有效方法

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-02 DOI: 10.3390/pathogens13090752

Safae Gouit, Ismahane Chair, Zineb Belabess, Ikram Legrifi, Khadija Goura, Abdessalem Tahiri, Abderrahim Lazraq, Rachid Lahlali

{"title":"Harnessing Trichoderma spp.: A Promising Approach to Control Apple Scab Disease","authors":"Safae Gouit, Ismahane Chair, Zineb Belabess, Ikram Legrifi, Khadija Goura, Abdessalem Tahiri, Abderrahim Lazraq, Rachid Lahlali","doi":"10.3390/pathogens13090752","DOIUrl":"https://doi.org/10.3390/pathogens13090752","url":null,"abstract":"Apple scab, caused by the pathogenic fungus Venturia inaequalis, can result in significant economic losses. The frequent use of fungicidal products has led to the emergence of isolates resistant to commonly used active substances. Therefore, biological control offers a sustainable alternative for managing apple scab. In this study, eight Trichoderma isolates were evaluated against five different isolates of V. inaequalis isolated from the Fes-Meknes region. The biocontrol potential of these Trichoderma isolates had previously been demonstrated against other pathogens. The results indicated that the inhibition rate of mycelial growth of V. inaequalis obtained with Trichoderma spp. isolates ranged from 50% to 81%, with significant differences observed among the pathogenic isolates after 5 and 12 days of incubation. In addition, the in vitro tests with Trichoderma cell-free filtrates showed inhibition rates ranging from 2% to 79%, while inhibition rates ranged from 5% to 78% for volatile compound tests. Interestingly, the inhibition of spore germination and elongation was approximately 40–50%, suggesting the involvement of antifungal metabolites in their biocontrol activities. The in vivo bioassay on detached apple leaves confirmed the biocontrol potential of these Trichoderma isolates and demonstrated their ability to preventively control apple scab disease. However, their efficacies were still lower than those of the fungicidal product difenoconazole. These findings could contribute to the development of an effective biofungicide based on these Trichoderma isolates for reliable and efficient apple scab control.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Over-Representation of Torque Teno Mini Virus 9 in a Subgroup of Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Pilot Study 肌痛性脑脊髓炎/慢性疲劳综合征患者亚群中转矩特诺迷你病毒 9 的过度代表性：一项试点研究

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-01 DOI: 10.3390/pathogens13090751

Karen Giménez-Orenga, Eva Martín-Martínez, Elisa Oltra

引用次数: 0

Bacteraemia Associated with Bilophila wadsworthia: A Rare Case Presentation from Hungary 与 Bilophila wadsworthia 相关的菌血症：匈牙利的一个罕见病例

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-31 DOI: 10.3390/pathogens13090749

Renátó Kovács, Tamás Árokszállási, Aliz Bozó, Ágnes Jakab, Krisztina Szonja Bábel, Máté Héja, Kitti Bernadett Kovács, Bence Balázs, Eszter Vitális, László Majoros, Zoltán Tóth

引用次数: 0

Involvement of MicroRNAs in the Hypersensitive Response of Capsicum Plants to the Capsicum Chlorosis Virus at Elevated Temperatures 微RNA参与辣椒植物在高温下对辣椒萎黄病病毒的超敏反应

IF 3.7 3区医学

Pathogens Pub Date : 2024-08-31 DOI: 10.3390/pathogens13090745

Wei-An Tsai, Christopher A. Brosnan, Neena Mitter, Ralf G. Dietzgen

{"title":"Involvement of MicroRNAs in the Hypersensitive Response of Capsicum Plants to the Capsicum Chlorosis Virus at Elevated Temperatures","authors":"Wei-An Tsai, Christopher A. Brosnan, Neena Mitter, Ralf G. Dietzgen","doi":"10.3390/pathogens13090745","DOIUrl":"https://doi.org/10.3390/pathogens13090745","url":null,"abstract":"The orthotospovirus capsicum chlorosis virus (CaCV) is an important pathogen affecting capsicum plants. Elevated temperatures may affect disease progression and pose a potential challenge to capsicum production. To date, CaCV-resistant capsicum breeding lines have been established; however, the impact of an elevated temperature of 35 °C on this genetic resistance remains unexplored. Thus, this study aimed to investigate how high temperature (HT) influences the response of CaCV-resistant capsicum to the virus. Phenotypic analysis revealed a compromised resistance in capsicum plants grown at HT, with systemic necrotic spots appearing in 8 out of 14 CaCV-infected plants. Molecular analysis through next-generation sequencing identified 105 known and 83 novel microRNAs (miRNAs) in CaCV-resistant capsicum plants. Gene ontology revealed that phenylpropanoid and lignin metabolic processes, regulated by Can-miR408a and Can- miR397, are likely involved in elevated-temperature-mediated resistance-breaking responses. Additionally, real-time PCR validated an upregulation of Can-miR408a and Can-miR397 by CaCV infection at HT; however, only the Laccase 4 transcript, targeted by Can-miR397, showed a tendency of negative correlation with this miRNA. Overall, this study provides the first molecular insights into how elevated temperature affects CaCV resistance in capsicum plants and reveals the potential role of miRNA in temperature-sensitive tospovirus resistance.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0