Open Medicine最新文献_第6页

Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy. 阴道镜检查诊断宫颈病变时宫颈细胞学阴性和 HPV 阳性的意义。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-02 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1051

Changhong Zhang, Liu Dong, Kejie Liu, Hong Xiao, Hao Si, Xiaoqin Wang, Hui Wang

{"title":"Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy.","authors":"Changhong Zhang, Liu Dong, Kejie Liu, Hong Xiao, Hao Si, Xiaoqin Wang, Hui Wang","doi":"10.1515/med-2024-1051","DOIUrl":"https://doi.org/10.1515/med-2024-1051","url":null,"abstract":"Objective: The aim of this study was to investigate the significance of colposcopy in diagnosing cervical lesions when negative cervical cytology is combined with positive human papillomavirus (HPV).Methods: Overall, 370 patients with cervical epithelial lesions who had negative cervical fluid-based cytology combined with positive HPV results were selected and analysed for severity of cervical lesions and HPV distribution.Results: Among the patients with cervical lesions, 242 had a single HPV infection, and 128 cases had multiple infections. No significant difference was found between HPV single infection and multiple infections in both groups of patients with cervical lesions (P > 0.05). Furthermore, 137 had non-HPV 16 and 18, accounting for 37.30% of all the patients with cervical lesions. Among them, HPV 52, 58, and 33 infections were the most common at 38.69, 30.66 and 29.20%, respectively - significantly higher than other high-risk HPV types (P < 0.05).Conclusion: High-risk HPV testing is crucial in patients with negative cervical fluid-based cytology combined with positive HPV results. Patients with HPV 16 and 18 and those with simple HPV 52, 58, and 33 infections should undergo timely colposcopy.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241051"},"PeriodicalIF":1.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis. FOXM1 和 AURKB 在调节银屑病角质细胞功能中的作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1049

Zhaofeng Zhao, Jie Cheng, Qiang Hou, Jian Zhu, Tu Chen, Sheng Lu, Guiju Wu, Hongli Lv, Xiujuan Wu

{"title":"Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis.","authors":"Zhaofeng Zhao, Jie Cheng, Qiang Hou, Jian Zhu, Tu Chen, Sheng Lu, Guiju Wu, Hongli Lv, Xiujuan Wu","doi":"10.1515/med-2024-1049","DOIUrl":"https://doi.org/10.1515/med-2024-1049","url":null,"abstract":"Objective: This study investigated the effect of forkhead box M1 (FOXM1) and Aurora kinase B (AURKB) on the epidermal function of keratinocytes.Methods: Bioinformatics analysis was used to analyze the co-expression network of FOXM1 and its correlation with AURKB. The expression of FOXM1 and AURKB in tissues and cells was detected by immunofluorescence and real-time quantitative polymerase chain reaction, respectively. HaCaT cells were transfected with si-FOXM1 to knock down FOXM1. Cell proliferation was detected by cell counting kit-8 assay. Cell migration was detected by scratch assay. Cell invasion was detected by the Transwell invasion assay. Cell apoptosis and cell cycle were detected by flow cytometry.Results: FOXM1 and AURKB were positively correlated and highly expressed in psoriatic lesions. After transfection of si-FOXM1, the expression levels of FOXM1 and AURKB genes significantly decreased. The proliferation of HaCaT cells decreased, the apoptosis rate increased significantly, and the proportion of cells in the G1 phase increased significantly, while the proportion of cells in the S phase decreased significantly. The scratch closure of HaCaT cells was reduced, and the number of cell invasions decreased significantly.Conclusion: FOXM1 and AURKB may affect the progression of psoriasis by regulating the proliferation, cell cycle, migration, and invasion of keratinocytes.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241049"},"PeriodicalIF":1.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parental control attitudes over their pre-school children's diet. 家长对学龄前儿童饮食的控制态度。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1054

Dulce Ivone Pinto Alves, Moniky Araújo da Cruz, Nadirlene Pereira Gomes, Amâncio António de Sousa Carvalho

{"title":"Parental control attitudes over their pre-school children's diet.","authors":"Dulce Ivone Pinto Alves, Moniky Araújo da Cruz, Nadirlene Pereira Gomes, Amâncio António de Sousa Carvalho","doi":"10.1515/med-2024-1054","DOIUrl":"https://doi.org/10.1515/med-2024-1054","url":null,"abstract":"It is during childhood that eating behaviors begin to form, with parents being the main agents in this process. Parents have eating habits that shape their children's diet, both in terms of variety and quantity of food eaten. The aim is to analyze sociodemographic factors related to parental control over their children's diet. Descriptive-correlational and cross-sectional study, with a sample of 46 parents of preschool children. An online questionnaire was used to collect data, with data processing carried out using SPSS, using descriptive and inferential statistics. The majority of respondents were mothers (89.1%), belonged to the 20-44 age group (89.1%), and were married (89.1%). The mean of the subscales of the children's food questionnaire food restriction, pressure to eat, and monitoring was 3.266 ± 0.570, 3.109 ± 1.206, and 4.268 ± 0.848, respectively. The mean rank score for the food restriction subscale differed significantly between parents with different age groups (Mann-Whitney: p < 0.014), with the 45-64 age group having the highest mean rank, i.e., they restricted their children more in food. The age group is a factor related to food restriction, making it essential to take a closer look at the parents of that age group, during the health education process.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241054"},"PeriodicalIF":1.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long intergenic noncoding RNA for IGF2BP2 stability suppresses gastric cancer cell apoptosis by inhibiting the maturation of microRNA-34a. 稳定 IGF2BP2 的长基因间非编码 RNA 通过抑制 microRNA-34a 的成熟抑制胃癌细胞凋亡

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-27 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-0992

Yao Wang, Zhigang Guo, Zhifeng Yang, Qingyan Deng, Yueming Huang, Yanhong Chen

{"title":"Long intergenic noncoding RNA for IGF2BP2 stability suppresses gastric cancer cell apoptosis by inhibiting the maturation of microRNA-34a.","authors":"Yao Wang, Zhigang Guo, Zhifeng Yang, Qingyan Deng, Yueming Huang, Yanhong Chen","doi":"10.1515/med-2024-0992","DOIUrl":"https://doi.org/10.1515/med-2024-0992","url":null,"abstract":"The oncogenic role of long intergenic noncoding RNA for IGF2BP2 stability (LINRIS) has been reported in colorectal cancer. This research aimed to study its potential involvement in gastric cancer (GC). In this study, paired GC and non-tumor tissues were obtained from 64 GC patients, and the levels of LINRIS, mature microRNA-34a (miR-34a), and miR-34a precursor in these tissues were measured with RT-qPCR. Linear regression was used to analyze their correlations. The role of LINRIS overexpression and siRNA silencing in regulating the maturation of miR-34a was analyzed by RT-qPCR. Cell apoptosis was studied with flow cytometry. It was observed that LINRIS was overexpressed in GC and showed a negative correlation with mature miR-34a, but not miR-34a precursor. In GC cells, LINRIS siRNA silencing upregulated mature miR-34a level, but not miR-34a precursor level. LINRIS overexpression downregulated miR-34a level. Cell apoptosis analysis showed that LINRIS siRNA silencing and miR-34a overexpression promoted GC cell apoptosis and suppressed cell migration and invasion, while LINRIS overexpression suppressed cell apoptosis and enhanced cell migration and invasion. In addition, the effect of LINRIS overexpression was reversed by miR-34a overexpression. Therefore, LINRIS siRNA silencing in GC may promote cell apoptosis by promoting miR-34a maturation.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20240992"},"PeriodicalIF":1.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The application of intravenous general anesthesia under nasopharyngeal airway assisted ventilation undergoing ureteroscopic holmium laser lithotripsy: A prospective, single-center, controlled trial. 输尿管镜钬激光碎石术中鼻咽气道辅助通气下静脉全身麻醉的应用：前瞻性单中心对照试验。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1046

Xuandong Jia, Min Wang

{"title":"The application of intravenous general anesthesia under nasopharyngeal airway assisted ventilation undergoing ureteroscopic holmium laser lithotripsy: A prospective, single-center, controlled trial.","authors":"Xuandong Jia, Min Wang","doi":"10.1515/med-2024-1046","DOIUrl":"https://doi.org/10.1515/med-2024-1046","url":null,"abstract":"To observe the effect of intravenous general anesthesia under nasopharyngeal airway-assisted ventilation on patients undergoing ureteroscopic holmium laser lithotripsy. One hundred and twenty patients who underwent ureteroscopic holmium laser lithotripsy in our hospital were selected and randomly divided into nasopharyngeal airway group (research group) and laryngeal mask group (control group). These patients, respectively, received intravenous anesthesia under nasopharyngeal airway and laryngeal mask-assisted ventilation. The following evaluation indexes were compared and analyzed between the two groups, including anesthetic effect, hemodynamics, stress response, postoperative recovery, adverse reactions, etc. There were no significant differences in Visual Analog Scale, hemodynamics, and stress response between the two groups at each time point (P > 0.05). There were no significant differences in residence time and postoperative recovery time between the two groups (P > 0.05). The difference in airway establishment time between the two groups was statistically significant (P < 0.05), and cases with blood in the research group was significantly lower than those in the control group (P < 0.05). Patient satisfaction in research group was significantly higher than those in the control group (P < 0.05). The clinical effect of intravenous general anesthesia under nasopharyngeal airway-assisted ventilation in ureteroscopic holmium laser lithotripsy is significant, which helps to stabilize patients' hemodynamics, reduce their stress response and adverse reactions, and improve the satisfaction rate of patient.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241046"},"PeriodicalIF":1.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11426383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tibial periosteum flap combined with autologous bone grafting in the treatment of Gustilo-IIIB/IIIC open tibial fractures. 胫骨骨膜瓣结合自体骨移植治疗 Gustilo IIIB/IIIC 开放性胫骨骨折。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-20 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1038

Yuling Gao, Yang Liu, Hongyu Hu, Shunhong Gao, Junlin Zhou

{"title":"Tibial periosteum flap combined with autologous bone grafting in the treatment of Gustilo-IIIB/IIIC open tibial fractures.","authors":"Yuling Gao, Yang Liu, Hongyu Hu, Shunhong Gao, Junlin Zhou","doi":"10.1515/med-2024-1038","DOIUrl":"10.1515/med-2024-1038","url":null,"abstract":"Purpose: Gustilo IIIB/C injuries are common for tibia diaphysis fractures with high rates of nonunion, osteomyelitis, and amputation. However, the managements on tibial Gustilo IIIB/C injuries are still controversial and individual. The aim of this study is to introduce the tibial periosteum flap combined with autologous bone grafting to treat Gustilo-IIIB/IIIC injuries.Methods: Sixteen Gustilo type IIIB/C tibial fracture patients who underwent tibial periosteum flaps with autologous bone grafting surgeries were retrospectively studied. In the first stage, the wound was treated with debridement and the fracture was reduced and fixed with an external fixator. After covering with vacuum sealing drainage for 7 days, the wound areas were repaired by flaps. When the flaps survived and external fixators were removed, the tibial periosteum flaps were taken with autologous bone grafting for bone defects.Results: The tibia fractures were comminuted fractures with mean size of segment bone defects 3.1 ± 1.3 cm. All the flaps survived and the wound healed in the first stage after an average of 1.5 ± 0.6 months. The mean size of the flap was 13.2 ± 2.8 cm × 7.3 ± 3.1 cm. All the autografts healed in 4.5 ± 0.7 months without infection and malunion. There was no pain in the affected limb. The weight-bearing and walking function were restored.Conclusion: Tibial periosteum flap combined with autologous bone grafting is effective to treat bone-skin defect of leg with Gustilo-IIIB/IIIC injury.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241038"},"PeriodicalIF":1.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sepsis induces the cardiomyocyte apoptosis and cardiac dysfunction through activation of YAP1/Serpine1/caspase-3 pathway 败血症通过激活 YAP1/Serpine1/caspase-3通路诱导心肌细胞凋亡和心脏功能障碍

IF 2.1 4区医学

Open Medicine Pub Date : 2024-09-18 DOI: 10.1515/med-2024-1018

Xueyuan Long, Yanpeng Yang, Ke Zhou

{"title":"Sepsis induces the cardiomyocyte apoptosis and cardiac dysfunction through activation of YAP1/Serpine1/caspase-3 pathway","authors":"Xueyuan Long, Yanpeng Yang, Ke Zhou","doi":"10.1515/med-2024-1018","DOIUrl":"https://doi.org/10.1515/med-2024-1018","url":null,"abstract":"Background Sepsis triggers myocardial injury and dysfunction, leading to a high mortality rate in patients. Cardiomyocyte apoptosis plays a positive regulatory role in septic myocardial injury and dysfunction. However, the mechanism is unclear. Methods Bioinformatics analysis was used to identify differentially expressed genes in septic mice heart and validate key genes and pathways. The correlation of protein–protein and protein–pathway was analyzed. Sequentially, the cecal ligament and puncture (CLP) was used to induce septic mice, followed by Serpine1 inhibitor treatment. Finally, the regulatory relationship of Yes-associated protein1 (YAP1), Serpine1, and caspase-3 was verified in LPS-exposed mouse cardiomyocytes. Results Bioinformatic analysis found that Serpine1 expression is decreased in septic mice heart tissue and closely related to the HIPPO signaling pathway, while YAP1 is negatively correlated with apoptosis. In vivo, CLP induced a reduction of survival rate, cardiac dysfunction, and an increase in Serpine1 and Cleaved Caspase-3 expression, which could be reversed by a Serpine1 inhibitor. In vitro, LPS induced the mouse cardiomyocytes apoptosis, which could be reversed by Serpine1 inhibitor. Silencing YAP1 and Serpine1 reversed the LPS-induced increase in Serpine1 and Cleaved Caspase-3 expression, but silencing Serpine1 did not affect the LPS-induced YAP1 expression. Conclusion Sepsis induced mouse cardiomyocytes apoptosis and cardiac dysfunction through activation of YAP1/Serpine1/caspase-3 pathway.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of iron metabolism and iron deficiency in incident patients on incident continuous ambulatory peritoneal dialysis 评估接受连续不卧床腹膜透析患者的铁代谢和缺铁情况

IF 2.1 4区医学

Open Medicine Pub Date : 2024-09-18 DOI: 10.1515/med-2024-1035

Qinghua Yin, Na Guo, Ping Fu, Hui Zhong

{"title":"Assessment of iron metabolism and iron deficiency in incident patients on incident continuous ambulatory peritoneal dialysis","authors":"Qinghua Yin, Na Guo, Ping Fu, Hui Zhong","doi":"10.1515/med-2024-1035","DOIUrl":"https://doi.org/10.1515/med-2024-1035","url":null,"abstract":"Objective The aim of this study was to investigate iron status and iron deficiency in incident continuous ambulatory peritoneal dialysis (CAPD) patients and identify influencing factors. Methods Patients with end-stage renal disease were enrolled. Clinical data of iron metabolism and biochemical and dialysis parameters during the first peritoneal dialysis evaluation were collected. Serum ferritin (SF) and transferrin saturation (TSAT) levels were evaluated, and independent influencing factors were identified by correlation and regression analyses. Results Of 1,128 adult CAPD patients, 41.2% had iron deficiency (ID), 15.7% had absolute iron deficiency, and 8.2% had functional iron deficiency. The average SF level was (276.8 ± 277.9) μg/L, and iron saturation was (29.8 ± 12.7)%. Additionally, 50.2 and 69.3% of patients reached targets in SF level and iron saturation recommended by the Chinese Society of Nephrology. SF level and TSAT were not correlated with estimated glomerular filtration rate, whereas negatively correlated with platelet count and inflammatory factors. Low platelet count, presence of diabetes mellitus and high interleukin 6 levels were independent factors of lower TSAT. Conclusions ID is common in patients with CAPD. Women and those with thrombocytopenia, diabetes, and inflammation are at higher risk for iron storage or reduced iron utilization. In the initial CAPD stage, a reasonable iron supplement strategy may be established for CAPD patients with high-risk factors.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p BIRC5-206 的减少通过海绵状 miR-145-5p 促进鼻咽癌的上皮-间质转化

IF 2.1 4区医学

Open Medicine Pub Date : 2024-09-18 DOI: 10.1515/med-2024-1007

Weihua Xu, Junjie Hu, Zhichao Ma, Wanyi Feng, Wei Gong, Shengmiao Fu, Xinping Chen

{"title":"Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p","authors":"Weihua Xu, Junjie Hu, Zhichao Ma, Wanyi Feng, Wei Gong, Shengmiao Fu, Xinping Chen","doi":"10.1515/med-2024-1007","DOIUrl":"https://doi.org/10.1515/med-2024-1007","url":null,"abstract":"Metastasis significantly contributes to the poor prognosis of advanced nasopharyngeal carcinoma (NPC). Our prior studies have demonstrated a decrease in BIRC5-206 expression in NPC, which promotes disease progression. However, the role of BIRC5-206 in the invasion and metastasis of NPC has not been fully elucidated. In this study, our objective was to explore the biological function and underlying mechanisms of BIRC5-206 in NPC. Additionally, we established an NPC mouse model of lung invasiveness using C666 cells to assess the impact of BIRC5-206 on NPC metastasis. Our results revealed that silencing BIRC5-206 inhibited apoptosis and enhanced the invasion of NPC cells, whereas its overexpression reversed these effects. Moreover, decreased BIRC5-206 expression significantly increased N-cadherin and Vimentin expression while reducing E-cadherin and occludin levels, both in vivo and in vitro. Additionally, silencing BIRC5-206 markedly augmented the formation of invasive foci in lung tissues. Rescue experiments further confirmed that decreased BIRC5-206 expression facilitates NPC metastasis via modulation of the miR-145-5p/CD40 signaling pathway. In summary, our study suggests that BIRC5-206 may serve as a potential prognostic biomarker and therapeutic target in the diagnosis and treatment of NPC.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"15 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway 地奥司钦通过TLR4/NF-κB途径预防慢性前列腺炎

IF 2.1 4区医学

Open Medicine Pub Date : 2024-09-13 DOI: 10.1515/med-2024-1036

Yan Long, Xiaodong Ge, Liangliang Ma, Junhua Guo, Yong Zhu

{"title":"Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway","authors":"Yan Long, Xiaodong Ge, Liangliang Ma, Junhua Guo, Yong Zhu","doi":"10.1515/med-2024-1036","DOIUrl":"https://doi.org/10.1515/med-2024-1036","url":null,"abstract":"This study aimed to elucidate the effects and potential mechanisms of dioscin on chronic prostatitis (CP) in vivo and in vitro. CP models were constructed in vivo and in vitro and treated with different concentrations of dioscin. Hematoxylin and eosin staining was used to investigate the morphology of the prostate tissues. The concentration of inflammatory factors in prostate tissues was determined by enzyme-linked immunosorbent assay. The release of reactive oxygen species, malondialdehyde, superoxide dismutase, and catalase was measured using detection kits. P69 cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Furthermore, the activity of the TLR4/NF-κB signaling pathway was determined by quantitative reverse transcriptase polymerase chain reaction or Western blot assay. Histopathological data suggested that dioscin exerted protective effects against prostate morphological changes. Dioscin inhibits inflammatory cytokines and oxidative stress (OS) in prostate tissues in a concentration-dependent manner. Moreover, dioscin notably inhibited the activation of the TLR4/NF-κB signaling pathway in CP rats. In vitro, dioscin remarkably reduced lipopolysaccharide-induced P69 proliferation, inflammation, OS, and TLR4/NF-κB pathway activation in a dose-dependent manner. In conclusion, dioscin exerts a protective effect in CP by decreasing the inflammatory response and OS through the TLR4/NF-κB pathways. Our findings provide a novel latent therapy for dioscin for the treatment and prevention of CP.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0