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Dexmedetomidine suppresses microglial activation in postoperative cognitive dysfunction via the mmu-miRNA-125/TRAF6 signaling axis. 右美托咪定通过mm - mirna -125/TRAF6信号轴抑制术后认知功能障碍中的小胶质细胞激活。
IF 1.6 4区 医学
Open Medicine Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1236
Zhiyan Xu, Kaihua Zhong, Weiyuan Chen, Huixia Lan, Weifeng Zhong, XiaoHong Wang, Mu Chen, Bin Pan
{"title":"Dexmedetomidine suppresses microglial activation in postoperative cognitive dysfunction via the mmu-miRNA-125/TRAF6 signaling axis.","authors":"Zhiyan Xu, Kaihua Zhong, Weiyuan Chen, Huixia Lan, Weifeng Zhong, XiaoHong Wang, Mu Chen, Bin Pan","doi":"10.1515/med-2025-1236","DOIUrl":"10.1515/med-2025-1236","url":null,"abstract":"<p><strong>Background: </strong>Postoperative cognitive dysfunction (POCD) is driven in part by microglial activation and the resulting neuroinflammatory response. Emerging evidence suggests that microRNAs regulate key inflammatory pathways in the central nervous system. In this study, we examined the role of the mmu‑miR‑125a/TRAF6 signaling axis in microglial activation under inflammatory conditions induced by lipopolysaccharide (LPS) and surgical trauma and evaluated whether dexmedetomidine (DEX) modulates this pathway to alleviate POCD.</p><p><strong>Methods: </strong>Murine microglial cells were treated with LPS to induce activation. Expression levels of mmu‑miR‑125a and TRAF6 were quantified by qRT‑PCR and Western blotting. Bioinformatic prediction of miRNA binding sites was performed, and a luciferase reporter assay was used to confirm direct targeting of TRAF6 by mmu‑miR‑125a. Adult mice underwent standardized surgical trauma to induce POCD. Brain tissues were analyzed for microglial activation markers, cytokine levels, and expression of mmu‑miR‑125a and TRAF6. DEX was administered in both <i>in vitro</i> and <i>in vivo</i> models. The effects on cytokine release, microglial activation, and the mmu‑miR‑125a/TRAF6 axis were assessed.</p><p><strong>Results: </strong>Our findings revealed significant alterations in the expression levels of TRAF6 and mmu-miR-125a during LPS-induced microglial activation. Through bioinformatics analysis and experimental validation, we identified TRAF6 as a direct target of mmu-miR-125a. The mmu-miR-125a/TRAF6 axis was found to be crucial for regulating microglial activation both <i>in vitro</i>, using an LPS-induced model, and <i>in vivo,</i> using a surgical trauma-induced POCD model. Moreover, we demonstrated that DEX, an alpha-2 adrenergic receptor agonist, effectively modulated the inflammatory cytokine release by targeting the mmu-miR-125a/TRAF6 axis in both models. The administration of DEX significantly suppressed microglial activation and TRAF6 expression, effects that were reversed by the inhibition of mmu-miR-125a.</p><p><strong>Conclusion: </strong>Our study provides new insights into the molecular mechanisms underlying microglial activation and highlights the therapeutic potential of targeting the mmu-miR-125a/TRAF6 axis to alleviate neuroinflammation by the administration of DEX in POCD.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251236"},"PeriodicalIF":1.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of serum metabolomics in patients with different types of chronic heart failure. 不同类型慢性心力衰竭患者血清代谢组学分析。
IF 1.6 4区 医学
Open Medicine Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1210
Bingzhang Jie, Qiang Li, Ling Han, Liwei Chen, Ming Yang
{"title":"Analysis of serum metabolomics in patients with different types of chronic heart failure.","authors":"Bingzhang Jie, Qiang Li, Ling Han, Liwei Chen, Ming Yang","doi":"10.1515/med-2025-1210","DOIUrl":"10.1515/med-2025-1210","url":null,"abstract":"<p><strong>Background: </strong>Heart failure remains a major public health issue, and there are still no reliable biomarkers for left ventricular ejection fraction (LVEF).</p><p><strong>Objective: </strong>To screen for differential metabolites in the blood of HFpEF, HFmrEF, and HFrEF patients based on metabolomics analysis of their blood samples.</p><p><strong>Methods: </strong>Total 44 patients in HFpEF group, 30 patients in HFmrEF group, and 36 patients in HFrEF group were selected. The blood metabolites were analyzed by liquid chromatography high-resolution mass spectrometry and classified by principal component analysis, and then potential biomarker were screened. Partial least squares discriminant analysis was used to model and investigate the predictive ability of biomarkers for LVEF.</p><p><strong>Results: </strong>Blood metabolite profiles of HFpEF, HFmrEF, and HFrEF groups could be well distinguished, and seven potential biomarkers were identified, such as phosphatidylcholine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylcholine, ceramide, sphingosine, and sphingomyelin. Four metabolic pathways, such as glycerol phospholipid metabolic pathway, linoleic acid metabolic pathway, purine pyrimidine metabolism pathway, and linolenic acid metabolism pathway were identified, among which glycerol phospholipid metabolism pathway was the most significant.</p><p><strong>Conclusion: </strong>The changes in glycerol phospholipid metabolism pathway may help identify HFpEF, HFmrEF, and HFrEF.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251210"},"PeriodicalIF":1.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Giant right atrial hemangioma presenting with ascites: A case report. 以腹水为表现的巨大右心房血管瘤1例。
IF 1.6 4区 医学
Open Medicine Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1169
Antonio Salsano, Giacomo Perocchio, Antonio Guadagno, Paolo Nozza, Tommaso Regesta, Francesco Santini
{"title":"Giant right atrial hemangioma presenting with ascites: A case report.","authors":"Antonio Salsano, Giacomo Perocchio, Antonio Guadagno, Paolo Nozza, Tommaso Regesta, Francesco Santini","doi":"10.1515/med-2025-1169","DOIUrl":"10.1515/med-2025-1169","url":null,"abstract":"<p><strong>Introduction: </strong>Cardiac hemangiomas are slow-growing benign tumours of the heart. Patients may be asymptomatic or present a multitude of signs or symptoms.</p><p><strong>Methods: </strong>We report herein the case of a 72-year-old woman with a giant right atrial mass. The patient suffers from abdominal swelling related to ascites. The histological examination of the tranjugular biopsy suspected an atrial myxoma.</p><p><strong>Results: </strong>The patient was scheduled for surgical excision of the cardiac tumour. Radical resection of a 13 cm mass was performed. The histological diagnosis revealed cardiac hemangioma.</p><p><strong>Conclusion: </strong>Cardiac hemangiomas can rarely grow larger than 5 cm, cause few symptoms, and are easily confused with atrial myxomas. Hepatomegaly and ascites may be signs of cardiac hemangioma.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251169"},"PeriodicalIF":1.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "The progress of autoimmune hepatitis research and future challenges". “自身免疫性肝炎研究进展和未来挑战”的勘误表。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-9993
Yang Zhang, Dehe Zhang, Ling Chen, Jing Zhou, Binbin Ren, Haijun Chen
{"title":"Corrigendum to \"The progress of autoimmune hepatitis research and future challenges\".","authors":"Yang Zhang, Dehe Zhang, Ling Chen, Jing Zhou, Binbin Ren, Haijun Chen","doi":"10.1515/med-2025-9993","DOIUrl":"10.1515/med-2025-9993","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1515/med-2023-0823.].</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20259993"},"PeriodicalIF":1.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12274966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance. 基于DNA测序的外籍献血者多系统罕见血型分子基因分型及其临床意义。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1234
Jianli Gong, Xianguo Xu, Jianrong Zhu
{"title":"Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance.","authors":"Jianli Gong, Xianguo Xu, Jianrong Zhu","doi":"10.1515/med-2025-1234","DOIUrl":"10.1515/med-2025-1234","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To establish a multi-level blood type identification system, comprehensively analyze the distribution characteristics and genetic polymorphisms of multi-system rare blood types in foreign blood donors, explore the application value of DNA sequencing technology in rare blood type screening, and evaluate its clinical significance in complex transfusion patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Blood samples from 277 foreign blood donors who participated in voluntary blood donation in Yiwu City were prospectively collected from June 2021 to March 2023. Serological typing of 24 antigens from 11 red blood cell blood group systems (ABO, Rh, Duffy, MNS, Kidd, Kell, Lutheran, P1PK, Lewis, H, and Diego) was performed using microcolumn agglutination and tube methods. First-generation sequencing technology was used to perform whole-exome sequencing of Duffy, Kell, Ss/GYPB, and Diego genes on screened rare phenotype samples to analyze genetic polymorphism characteristics. Key mutation sites were verified using multiplex PCR-sequencing. A rare blood type DNA database was established and compared with the international blood group gene database (BGMUT). Confirmed rare blood type units were preserved through programmed freezing, and their clinical application effects were tracked and analyzed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The 277 foreign blood donors were primarily from the Middle East and South Asia (71.8%), with major source countries including Syria (56 cases, 20.22%), Yemen (49 cases, 17.69%), Pakistan (24 cases, 8.66%), Iraq (20 cases, 7.22%), India (15 cases, 5.42%), Iran (14 cases, 5.05%), Mali (11 cases, 3.97%), and Jordan (10 cases, 3.61%). In blood type distribution, Fya antigen expression was highest among Indian (100%) and Pakistani (87.50%) donors; 63 cases of Fy(a-b-) were found, most commonly in donors from Mali and Yemen. S antigen expression was highest in donors from Syria (60.71%), India (60.00%), and Pakistan (58.33%); 47 cases of S+s- were detected. Additionally, 12 cases of Lua+ were found, distributed among Syria (3 cases), Iraq (2 cases), Yemen (2 cases), Jordan (2 cases), etc.; 5 cases of Kpa+ were from Yemen (2 cases), Pakistan, Iraq, and Jordan (1 case each). DNA sequencing revealed that GATA-1 promoter region mutation (c.-67T&gt;C) in the Duffy gene was the primary molecular basis for the Fy(a-b-) phenotype, accounting for 96.8% (61/63). Multivariate analysis demonstrated significant clustering of blood group phenotypes by geographical regions (&lt;i&gt;p&lt;/i&gt; &lt; 0.001), with the first two principal components explaining 78.3% of the variance in distribution patterns. Genotype-phenotype correlation analysis showed a concordance rate of 99.2% (248/250). During the study period, 41 rare phenotype blood units (74U) were screened and cryopreserved, including 14 units (24.5U) of Fy(a-b-), 25 units (45.5U) of Fy(a-b+), and 2 units (4.0U) of s(-). In clinical application, these units were successfully used in three di","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251234"},"PeriodicalIF":1.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The identification of novel missense variant in ChAT gene in a patient with gestational diabetes denotes plausible genetic association. 在妊娠糖尿病患者中发现新的ChAT基因错义变异表明可能的遗传关联。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1225
Oluwafemi G Oluwole, Afolake Arowolo, Ezekiel Musa, Naomi Levitt, Mushi Matjila
{"title":"The identification of novel missense variant in <i>ChAT</i> gene in a patient with gestational diabetes denotes plausible genetic association.","authors":"Oluwafemi G Oluwole, Afolake Arowolo, Ezekiel Musa, Naomi Levitt, Mushi Matjila","doi":"10.1515/med-2025-1225","DOIUrl":"10.1515/med-2025-1225","url":null,"abstract":"<p><strong>Introduction: </strong>Gestational diabetes mellitus (GDM), the most common metabolic complication of pregnancy, is associated with a 50% increase in subsequent risk for type 2 diabetes. There is increasing interest in identifying biomarkers that may facilitate the stratification of subsequent type 2 diabetes risk among women with GDM. In this study, we considered the choline acetyltransferase (<i>ChAT</i>) gene. CHAT plays a critical role in acetylcholine synthesis and regulates insulin secretion from the pancreatic islet to maintain glucose homeostasis.</p><p><strong>Methods: </strong>We screened for deleterious variants in the <i>ChAT</i> gene in 12 GDM patients and 10 ethnically matched controls from a South African cohort. We isolated DNA from the placental samples of these patients and performed DNA sequencing of the protein-coding region of the <i>ChAT</i> gene. Sequence alignments and variant annotations were done using UGENE software and Ensembl VEP.</p><p><strong>Results: </strong>A novel heterozygous missense variant in exon 8 of the <i>ChAT</i> gene was identified. The plausible phenotypic impact of the variant <i>ChAT</i> (NM_020549.5):c.1213C>G (p.Leu405Val) can be explained by haploinsufficiency, changing protein activities, strong transcription activity, and epigenetic repression activities of the variant. Also, structurally, the variant is located 18bp in-frame to a stop-gained variant (p.Gly411Ter). The RegulomeDB DNase expression data clearly show the identified variant in a peak expression in the spleen and placenta. This observation corroborates that the <i>ChAT</i> gene may play an essential role in GDM.</p><p><strong>Conclusion: </strong>Taken together, the metric scores for this variant show that it could affect the functions of the gene, but more functional studies are necessary to validate these effects. Consequently, this study sets the stage for the future screening of a larger cohort and functional validation of deleterious variants to underpin the <i>ChAT</i> gene and GDM association.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251225"},"PeriodicalIF":1.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intra-abdominal hypertension/abdominal compartment syndrome of pediatric patients in critical care settings. 危重病儿科患者的腹内高压/腹腔隔室综合征
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1244
Vesna G Marjanovic, Ivana Z Budic, Maja D Zecevic, Marija M Stevic, Dusica M Simic
{"title":"Intra-abdominal hypertension/abdominal compartment syndrome of pediatric patients in critical care settings.","authors":"Vesna G Marjanovic, Ivana Z Budic, Maja D Zecevic, Marija M Stevic, Dusica M Simic","doi":"10.1515/med-2025-1244","DOIUrl":"10.1515/med-2025-1244","url":null,"abstract":"<p><strong>Background: </strong>Intra-abdominal hypertension (IAH)/abdominal compartment syndrome (ACS) is one of the rarer clinical entities in the pediatric population, carrying a high degree of morbidity and mortality. The focus of this review is on assessing pathophysiological changes of organ systems in pediatric patients with risk factors for the occurrence of IAH/ACS based on the evaluation of diagnostic modalities and therapeutic strategies.</p><p><strong>Methodology: </strong>A comprehensive literature search of indexed databases was performed, aiming to identify, review, and evaluate published articles on IAH/ACS. The search was focused on studies examining pathophysiology, risk factors, diagnostic approaches, and management strategies.</p><p><strong>Results: </strong>The main risk factors encompass diminished abdominal wall compliance, increased intraluminal and abdominal contents, and capillary leak/fluid resuscitation. Diagnostic tools include clinical and imaging findings, intra-abdominal pressure (IAP) monitoring, and parameters of tissue perfusion. Therapeutic strategies involve non-surgical and surgical management of IAH/ACS in pediatric patients.</p><p><strong>Conclusion: </strong>Timely and continuous evaluation of IAP and parameters of tissue perfusion is crucial for the early diagnosis of IAH/ACS and implementing non-surgical procedures, reducing the need for surgical procedures. Future research should focus on the usefulness of advanced non-invasive monitoring technologies and the identification of predictors of increased IAP in the early implementation of personalized therapeutic strategies.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251244"},"PeriodicalIF":1.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the role of succinyl carnitine in the association between CD39⁺ CD4⁺ T cell and ulcerative colitis: A Mendelian randomization study. 探索琥珀酰肉碱在CD39 + CD4 + T细胞与溃疡性结肠炎相关性中的作用:一项孟德尔随机研究。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1240
Li Chen, Ying Yi, Yun Zhu
{"title":"Exploring the role of succinyl carnitine in the association between CD39⁺ CD4⁺ T cell and ulcerative colitis: A Mendelian randomization study.","authors":"Li Chen, Ying Yi, Yun Zhu","doi":"10.1515/med-2025-1240","DOIUrl":"10.1515/med-2025-1240","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the potential causal relationship between immune cell and the risk of ulcerative colitis (UC), and to explore whether serum metabolites may mediate this association, thereby suggesting potential biomarkers or therapeutic targets.</p><p><strong>Methods: </strong>We conducted a Mendelian randomization (MR) analysis using summary statistics from genome-wide association studies to evaluate both the direct effects and potential mediating roles of 731 immune cells and 1,400 serum metabolites in relation to UC. Instrumental variables were rigorously selected based on genome-wide significance and linkage disequilibrium thresholds. The primary analytical method was inverse variance weighted, supplemented by MR-Egger regression and weighted median methods to ensure robustness. Cochran's <i>Q</i> test, MR-Egger intercept, and leave-one-out analysis were employed to evaluate heterogeneity and pleiotropy. Mediation MR analysis was conducted to examine potential metabolite-mediated pathways.</p><p><strong>Results: </strong>We identified a statistically significant positive causal effect of CD39⁺ CD4⁺ T cell on UC risk (OR = 1.05, 95% CI = 1.03-1.08, <i>beta_all</i> = 0.05). Sensitivity analyses confirmed the robustness of this association, and reverse MR analysis indicated no causal effect of UC on CD39⁺ CD4⁺ T cell, suggesting a unidirectional relationship. Mediation analysis further revealed that succinyl carnitine (C4DC) partially mediated the effect of CD39⁺ CD4⁺ T cell on UC, with a mediation proportion of 3.3%.</p><p><strong>Conclusion: </strong>Our findings suggest that CD39⁺ CD4⁺ T cell may increase the risk of UC, potentially by modulating the levels of succinyl carnitine (C4DC). These results indicate a potential immunometabolic pathway in UC pathogenesis and highlight CD39⁺ CD4⁺ T cell and C4DC as promising targets for further research. However, additional experimental validation is required to confirm these findings and assess their clinical relevance.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251240"},"PeriodicalIF":1.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curdione protects vascular endothelial cells and atherosclerosis via the regulation of DNMT1-mediated ERBB4 promoter methylation. Curdione通过调节dnmt1介导的ERBB4启动子甲基化来保护血管内皮细胞和动脉粥样硬化。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-14 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1223
Yingbiao Wu, Can Jin, Luoning Zhu, Xiaogang Zhang, Xinpeng Cong, Budian Xing, Zhongping Ning
{"title":"Curdione protects vascular endothelial cells and atherosclerosis via the regulation of DNMT1-mediated ERBB4 promoter methylation.","authors":"Yingbiao Wu, Can Jin, Luoning Zhu, Xiaogang Zhang, Xinpeng Cong, Budian Xing, Zhongping Ning","doi":"10.1515/med-2025-1223","DOIUrl":"10.1515/med-2025-1223","url":null,"abstract":"<p><p>Atherosclerosis (AS) is initiated by the activation of the endothelial cells, which is followed by a series of events that trigger the narrowing of blood vessels and the activation of inflammation. This study aimed to investigate <i>in vitro</i> the roles and underlying mechanisms of curdione in AS. Human umbilical vein endothelial cells (HUVECs) were stimulated with oxidized low-density lipoprotein (ox-LDL) and then treated with curdione, after which the growth of the HUVECs and the related mechanisms were determined. HUVECs with ERBB4 overexpression were constructed to explore the role of ERBB4 in curdione-mediated AS. The interaction among ERBB4, methylation, and curdione was confirmed by chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) and dual luciferase reporter gene assays. Both curdione and ERBB4 overexpression individually and significantly enhanced viability and proliferation while suppressing apoptosis of the ox-LDL-induced HUVECs, and the combination of curdione and ERBB4 overexpression had better effects. Compared with the ox-LDL-induced HUVECs, both curdione and ERBB4 overexpression individually decreased the levels of IL-6, IL-1β, and IL-8 (<i>P</i> < 0.05). They also upregulated Bax, caspase-3, E-cadherin, and F-actin while downregulating Bcl-2 and VEGF (<i>P</i> < 0.05). Additionally, the ERBB4 bound to the DNMT1 gene, and the curdione participated in AS via the ERBB4 gene. The study demonstrated that either curdione or ERBB4 overexpression individually may ameliorate AS development by inhibiting apoptosis, inflammation, and the EndMT of HUVECs. In addition, curdione may protect the vascular endothelial cells and AS by regulating the DNMT1-mediated ERBB4 promoter methylation.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251223"},"PeriodicalIF":1.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of computed tomography and guided bronchoscopy in the diagnosis of pulmonary nodules: A systematic review and meta-analysis. 计算机断层扫描和引导支气管镜在肺结节诊断中的比较:系统回顾和荟萃分析。
IF 1.7 4区 医学
Open Medicine Pub Date : 2025-07-11 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1108
Yilin Wang, Xianhe Liang, Haitao Zhang, Jinhua Hao, Min Wei
{"title":"Comparison of computed tomography and guided bronchoscopy in the diagnosis of pulmonary nodules: A systematic review and meta-analysis.","authors":"Yilin Wang, Xianhe Liang, Haitao Zhang, Jinhua Hao, Min Wei","doi":"10.1515/med-2024-1108","DOIUrl":"10.1515/med-2024-1108","url":null,"abstract":"<p><strong>Objective: </strong>To compare the diagnostic effects of computed tomography (CT) and bronchoscopy guided biopsy on pulmonary nodules.</p><p><strong>Methods: </strong>\"Subject words + free words\" were used to search four literature databases including Pubmed, Embase, Cochrane Library, and Web of Science. The subject words were CT, bronchoscope-guided biopsy, pulmonary nodules, and diagnosis. The search is up to August 25, 2023. The retrieved literature was screened, and the literature with duplicate, review, incomplete data or article content and inconsistent with our research was deleted. Revman 5.4 was used for bias analysis of the literatures included in the analysis, and forest map, funnel map, and ROC curve were drawn to compare the accuracy, specificity, and sensitivity of the two diagnostic methods.</p><p><strong>Results: </strong>A total of 2,622 articles were retrieved, and 8 articles were included in the study after screening. Bias analysis and funnel plot showed that eight included articles had higher quality and smaller bias. The sensitivity and specificity of the forest map were both 95% CI, and the sensitivity of CT diagnosis was better than that of bronchoscopy diagnosis, with similar specificity between the two groups. The funnel plot shows some heterogeneity in the literature on pulmonary nodules. The ROC curve shows that CT diagnosis is significantly superior to bronchoscopy diagnosis, with diagnostic accuracy approaching 100%.</p><p><strong>Conclusion: </strong>CT has significant advantages in detecting and diagnosing pulmonary nodules, as it can detect nodules in various parts of the lungs. However, bronchoscopy has significant advantages in diagnosing small pulmonary nodules.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241108"},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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