Open Medicine最新文献

{"title":"Mechanism research on inhibition of gastric cancer <i>in vitro</i> by the extract of <i>Pinellia ternata</i> based on network pharmacology and cellular metabolomics.","authors":"Fan Feng, Ping Hu, Jun Chen, Lei Peng, Luyao Wang, Xingkui Tao, Chaoqun Lian","doi":"10.1515/med-2024-1131","DOIUrl":"10.1515/med-2024-1131","url":null,"abstract":"<p><strong>Background and purpose: </strong>Gastric cancer is a kind of malignant tumor with high incidence and high mortality, which has strong tumor heterogeneity. A classic Chinese medicine, <i>Pinellia ternata</i> (PT), was shown to exert therapeutic effects on gastric cancer cells. However, its chemical and pharmacological profiles remain to be elucidated. In the current study, we aimed to reveal the mechanism of PT in treating gastric cancer cells through metabolomic analysis and network pharmacology.</p><p><strong>Methods: </strong>Metabolomic analysis of two strains of gastric cancer cells treated with the <i>Pinellia ternata</i> extract (PTE) was used to identify differential metabolites, and the metabolic pathways were enriched by MetaboAnalyst. Then, network pharmacology was applied to dig out the potential targets against gastric cancer cells induced by PTE. The integrated network of metabolomics and network pharmacology was constructed based on Cytoscape.</p><p><strong>Results: </strong>The PTE was confirmed to significantly inhibit cell proliferation, migration, and invasion of HGC-27 and BGC-823 cells. The results of cellular metabolomics showed that 61 metabolites were differently expressed in gastric cancer cells of the experimental and control groups. Through pathway enrichment analysis, 16 metabolites were found to be involved in the glycerophospholipid metabolism, purine metabolism, sphingolipid metabolism, and tryptophan metabolism. Combined with network pharmacology, seven bioactive compounds were found in PT, and the networks of bioactive compound-target gene-metabolic enzyme-metabolite interactions were constructed.</p><p><strong>Conclusions: </strong>In conclusion, this study revealed the complicated mechanisms of PT against gastric cancer. Our work provides a novel paradigm to identify the potential mechanisms of pharmacological effects derived from a natural compound.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241131"},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of <i>Lycium barbarum</i> polysaccharide on bone cell dynamics in osteoporosis.","authors":"Qing Wang, Ting Zhang, Xinting Feng, Peng Chen, Ye Feng, Haoqiang Huang, Yinhua Qian, Yang Guo, Zifei Yin","doi":"10.1515/med-2024-1104","DOIUrl":"10.1515/med-2024-1104","url":null,"abstract":"<p><strong>Background: </strong>Osteoporosis (OP) is a systemic bone disorder marked by reduced bone mass and disrupted microstructure, leading to higher fracture risk. Epidemiological data from China show a 20.7% prevalence in women and 14.4% in men over 50, underscoring a pressing health issue given the aging population. More drugs to inhibit OP progression should be explored, and their biological mechanisms confirmed in preclinical studies.</p><p><strong>Methods: </strong>In this study, we utilized <i>Lycium barbarum</i> polysaccharide (LBP), an extract from the traditional Chinese medicine Goji Berry. LBP, known for its range of pharmacological activities, was assessed for its potential therapeutic effects on OP. We specifically investigated its influence on the proliferation, apoptosis, migration, and functional differentiation of osteoblasts and osteoclasts.</p><p><strong>Results: </strong>LBP significantly promotes osteoblast proliferation, migration, and osteogenic differentiation. Conversely, it inhibits the intrinsic apoptotic response in osteoblasts. For osteoclasts, LBP suppressed their proliferation, migration, and osteoclastic differentiation while enhancing their natural apoptosis. These results were confirmed by classical protein pathway detection experiments.</p><p><strong>Conclusion: </strong>LBP showcases potential therapeutic properties against OP, particularly in modulating osteoblast/osteoclast activities. While its exact mechanisms through vital signaling pathways remain to be fully elucidated, LBP's prominent effects suggest that it is a promising agent for OP intervention, warranting further in-depth studies.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241104"},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Empagliflozin improves aortic injury in obese mice by regulating fatty acid metabolism\".","authors":"Lin Yue, Yue Wang, Cuiying Wang, Shu Niu, Xihong Dong, Yaqing Guan, Shuchun Chen","doi":"10.1515/med-2025-9999","DOIUrl":"https://doi.org/10.1515/med-2025-9999","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1515/med-2024-1012.].</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20259999"},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic changes and gross morphology of placenta in women affected by gestational diabetes mellitus in dietary treatment: A systematic review.","authors":"Rossella Molitierno, Amalia Imparato, Nicola Iavazzo, Cecilia Salzillo, Andrea Marzullo, Antonio Simone Laganà, Andrea Etrusco, Vittorio Agrifoglio, Antonio D'Amato, Esposito Renata, Maria Giovanna Vastarella, Pasquale De Franciscis, Marco La Verde","doi":"10.1515/med-2025-1142","DOIUrl":"10.1515/med-2025-1142","url":null,"abstract":"<p><strong>Introduction/objective: </strong>Gestational diabetes mellitus (GDM) influences adverse maternal and fetal outcomes. Nutritional therapy and exercise are the first steps to maintain normal glucose levels. During pregnancy, metabolic status influences placental development.</p><p><strong>Methods: </strong>This systematic review focused only on the morphology of the placenta and its microscopic changes in GMD under dietary therapy. A systematic search was performed on the main databases from inception to September 2024 (PROSPERO ID: CRD42024581621). Only original articles on GDM in diet and exercise treatment that reported at least one outcome of interest (microscopic features and macroscopic morphology of the placenta) were included.</p><p><strong>Results: </strong>A total of 716 studies were identified, and nine met the inclusion criteria. The analysis confirmed that despite dietary control, some morphological changes in the placenta, including villus immaturity, chorangiosis, and fibrinoid necrosis, occurred at a different rate. In addition, the included studies reported an increase in placental weight in the diet-controlled GDM group.</p><p><strong>Conclusion: </strong>Therefore, the results of the present qualitative analysis show that pregnant women with diet-controlled GDM, despite adequate glycemic control, abnormal placental development may persist. Our findings remark on the importance of the correct diet-managed GDM pregnancy monitoring due to the placental morphology abnormalities related to GMD.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251142"},"PeriodicalIF":1.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health literacy and health-related quality of life: The mediating role of irrational happiness.","authors":"Amil Huseynov, Begum Satici","doi":"10.1515/med-2025-1148","DOIUrl":"10.1515/med-2025-1148","url":null,"abstract":"<p><p><b>Background:</b> Health literacy (HL) and irrational beliefs about happiness significantly influence adults' health-related quality of life (HRQoL). The purpose of this study was to examine whether irrational happiness mediates the relationship between HL and HRQoL among adults. <b>Methods:</b> A total of 686 adults (468 women and 218 men; mean age = 22.30  ±  6.83 years) completed self-report questionnaires, including the Health Literacy Scale-Short Form, the EUROHIS-QOL 8, and the Irrational Happiness Beliefs Scale. Data were analyzed using structural equation modeling and bootstrapping methods. <b>Results:</b> HL had both direct (<i>β</i> = 0.260, <i>p</i> < 0.01) and indirect effects on HRQoL. HL directly predicted irrational happiness (<i>β</i> = -0.369, <i>p</i> < 0.01), which in turn directly predicted HRQoL (<i>β</i> = -0.318, <i>p</i> < 0.01). Specifically, irrational happiness significantly mediated the relationship between HL and HRQoL (bootstrap coefficient = 0.117, 95% CI = 0.071-0.175). <b>Conclusion:</b> These findings suggest that interventions aiming to enhance adults' HRQoL should consider not only improving HL but also addressing irrational beliefs about happiness.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251148"},"PeriodicalIF":1.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The era of increasing cancer survivorship: Trends in fertility preservation, medico-legal implications, and ethical challenges.","authors":"Lina De Paola, Gabriele Napoletano, Giuseppe Gullo, Francesco Circosta, Gianluca Montanari Vergallo, Susanna Marinelli","doi":"10.1515/med-2025-1144","DOIUrl":"10.1515/med-2025-1144","url":null,"abstract":"<p><strong>Introduction: </strong>Global cancer cases are increasing, but fortunately, cancer is becoming more treatable. By 2050, the number of cancer cases is projected to reach 35 million. These numbers are certainly correlated with the aging population, early diagnoses due to screenings, and the broad current treatment options. However, life-saving therapies are often gonadotoxic, significantly impacting the lives of cancer patients. Fertility preservation following life-saving oncological treatments is one of the challenges faced by patients with cancer.</p><p><strong>Material and method: </strong>We analyzed 73 articles to investigate the current state of fertility preservation in oncology, also evaluating the medico-legal implications.</p><p><strong>Results: </strong>The data indicate a growing trend of cancer recoveries and survivorship with opportunities to access fertility preservation through various methods, which are not entirely known or consistently offered to patients in the appropriate manner.</p><p><strong>Conclusions: </strong>The ethical and medico-legal aspects are numerous and seem to be still evolving.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251144"},"PeriodicalIF":1.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed graft function after renal transplantation.","authors":"Darijus Skaudickas, Povilas Lenčiauskas, Augustas Skaudickas, Andrejus Bura","doi":"10.1515/med-2025-1140","DOIUrl":"10.1515/med-2025-1140","url":null,"abstract":"<p><strong>Background information: </strong>Delayed graft function (DGF), defined as the need for dialysis within the first week of a kidney transplant, is a common complication, particularly in extended criteria donor transplants, where its incidence ranges from 21 to 31%.</p><p><strong>Objectives: </strong>We observed a prolonged case of DGF in a 47-year-old patient with chronic kidney disease (CKD) resulting from diabetic nephropathy. The patient, classified in a moderate immunologic mismatch group, received a marginal deceased donor kidney.</p><p><strong>Results: </strong>For the first 4 weeks post-transplantation, graft function was impaired. After 29 days of anuria, the transplanted kidney began to recover. The literature review found few clinical cases of DGF extending beyond 1 month. Our patient had several risk factors for DGF, including diabetes mellitus, pre-transplant hemodialysis, and moderate immunologic mismatch. Additionally, the marginal graft increased the risk of ischemia-reperfusion injury and glycocalyx damage. However, it remains unclear how these factors influenced the duration of DGF. The exact cause of the extended DGF in this case remains unknown. Although the literature identifies key risk factors for DGF, data on factors leading to prolonged kidney dysfunction are lacking. Therefore, decisions to remove a non-functioning transplanted kidney should not be made hastily.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251140"},"PeriodicalIF":1.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first-line antihypertensive nitrendipine potentiated the therapeutic effect of oxaliplatin by downregulating CACNA1D in colorectal cancer.","authors":"Chengzhe Lai, Jinghu Liu, Jingna Zhou, Haokun Zhou","doi":"10.1515/med-2024-1138","DOIUrl":"10.1515/med-2024-1138","url":null,"abstract":"<p><strong>Background: </strong>Oxaliplatin (OXA) is among the most common chemotherapy drugs and is the base component of the FOLFOX regimen (OXA + leucovorin + 5-fluorouracil) and CapeOX regimen (OXA + capecitabine). Resistance to and failure of these two OXA-based regimens often results in poor outcomes in patients with colorectal cancer (CRC). Nitrendipine (NTD) is a first-line antihypertensive drug commonly used in hypertension and coronary heart disease with confirmed low toxicity and side effects. However, the potential benefits of NTD for CRC progression and therapy remain unclear.</p><p><strong>Methods: </strong>Cell counting kit-8 (CCK-8) detection, colony formation assay, wound-healing assay, Transwell assay, SynergyFinder webtool, and subcutaneous tumor models were used to assess the effect of NTD with OXA on CRC inhibition <i>in vitro</i> and <i>in vivo</i>. Bioinformatics tools including Human Protein Atlas (HPA), quantitative real-time polymerase chain reaction, western blotting analyses, lentivirus transfection, and rescue experiment were used to investigate the mechanism(s) of the related action.</p><p><strong>Results: </strong>Utilizing murine and human CRC cell lines, the <i>in vitro</i> and <i>in vivo</i> experiment demonstrated that NTD inhibited cell proliferation, migration, and invasion, and the synergy scores calculated by SynergyFinder indicated that NTD exhibited synergistic activity with the chemotherapeutic drug OXA. The CCK-8 detection, animal model, and rescue experiment results demonstrated that NTD suppressed CRC progression and potentiated OXA therapeutic effect by downregulating calcium voltage-gated channel subunit alpha1 D (CACNA1D).</p><p><strong>Conclusions: </strong>This study presents novel data on first-line antihypertensive NTD, exerting inhibitory effects on cell proliferation and migration in CRC and revealing synergistic activity with OXA by downregulating CACNA1D. NTD may be a candidate as a promising chemosensitizer as an OXA new combination to improve the efficacy and safety of CRC therapy.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241138"},"PeriodicalIF":1.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D replacement therapy may regulate sleep habits in patients with restless leg syndrome.","authors":"Feyza Ustabaş Kahraman, Ayşegül Doğan Demir, Mebrure Yazıcı, Fatma Betül Çakır, Hayrettin Temel, Abdülhamit Çollak, Ufuk Erenberk","doi":"10.1515/med-2024-1125","DOIUrl":"10.1515/med-2024-1125","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study is to determine the effect of vitamin D replacement therapy on sleep habits in restless legs syndrome (RLS) patients with vitamin D deficiency.</p><p><strong>Methods: </strong>The study was conducted between September 1, 2018, and September 1, 2019, at the Pediatrics outpatient clinic of Bezmialem Vakif University. 46 cases with RLS and 43 healthy controls between these dates were included in the study. The Children's Sleep Habits Questionnare was filled out retrospectively by parents.</p><p><strong>Results: </strong>While vitamin D supplementation was given to 20 children with RLS who were found to have vitamin D deficiency, the 26 patients without vitamin D deficiency did not receive supplementation. There was no significant difference between the treatment groups in terms of gender, age, and pre-treatment sleep habits. Compared to the pre-treatment period, post-treatment bedtime resistance, sleep onset delay, sleep anxiety, parasomnias, daytime sleepiness, and CSHQ total score were significantly improved in RLS cases with vitamin D deficiency. There were no significant differences in RLS patients without vitamin D supplementation. Other comparisons also showed no difference between groups.</p><p><strong>Conclusion: </strong>There was no difference between the sleep habits of RLS cases with and without vitamin D deficiency. Sleep habits improved positively after vitamin D supplementation in patients with vitamin D deficiency compared to the pre-treatment period. There was no difference in the sleeping habits of RLS patients without vitamin D deficiency during the same period. Vitamin D supplementation can improve sleep habits in patients with RLS.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241125"},"PeriodicalIF":1.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydromorphone reduced the incidence of emergence agitation after adenotonsillectomy in children with obstructive sleep apnea: A randomized, double-blind study.","authors":"Qiyuan Huang, Yang Chen, Xiaohui Sun, Yongwei Su, Ruihao Zhou, Guo Chen, Tao Zhu","doi":"10.1515/med-2024-1129","DOIUrl":"10.1515/med-2024-1129","url":null,"abstract":"<p><strong>Purpose: </strong>Emergence agitation (EA) after (adeno)tonsillectomy (AT) surgery impairs recovery in children. Adequate analgesia plays a crucial role in reducing EA incidence. This study investigated whether hydromorphone infusion (30 μg/kg) during anesthesia induction could reduce EA following AT surgery for obstructive sleep apnea in children.</p><p><strong>Patients and methods: </strong>A total of 186 ASA I-III children aged 3-7 years undergoing AT surgery were enrolled in a blinded randomized trial comparing hydromorphone (30 μg/kg) to fentanyl (4 μg/kg). The primary outcome was EA incidence within 30 min post-extubation. Secondary outcomes included pediatric anesthesia emergence delirium (PAED), face, legs, activity, crying, consolability (FLACC), Ramsay sedation scores, extubation time, rescue analgesia incidence, and adverse events.</p><p><strong>Results: </strong>The incidence of EA was significantly lower in the hydromorphone group [48.4% (45/93) vs 64.5% (60/93); absolute difference: 16.1%; 95% CI: 18.9-29.5%; <i>P</i> = 0.027]. Hydromorphone improved PAED, FLACC, and Ramsay scores and reduced moderate-to-severe pain and rescue analgesia. No postoperative complications occurred in either group.</p><p><strong>Conclusion: </strong>Hydromorphone at 30 μg/kg effectively reduces the incidence of EA within 30 min post-extubation in children after AT surgery compared to fentanyl. It shows superior analgesia and has a low incidence of adverse effects.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241129"},"PeriodicalIF":1.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}