Open Medicine最新文献_第5页

Maternal factors for neural tube defects in offspring: An umbrella review. 后代神经管缺陷的母体因素：综述。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1061

Hoda Arabzadeh, Ensiyeh Jenabi, Seyedeh Zahra Masoumi

{"title":"Maternal factors for neural tube defects in offspring: An umbrella review.","authors":"Hoda Arabzadeh, Ensiyeh Jenabi, Seyedeh Zahra Masoumi","doi":"10.1515/med-2024-1061","DOIUrl":"10.1515/med-2024-1061","url":null,"abstract":"Objective: We conducted an umbrella review focusing on maternal risk factors during pregnancy associated with neural tube defects (NTDs).Methods: Our search was in databases PubMed, Scopus, and Web of Science. We specifically targeted meta-analyses examining maternal factors during pregnancy in relation to NTDs. The comparison involved assessing metrics such as odds ratio (OR) or related risk ratios reported in the included studies, as well as parameters like heterogeneity (I²), 95% prediction interval, small-study effects, excess significance biases, and sensitivity analysis.Results: Three risk factors for fetal NTDs, namely hyperthermia with an OR of 1.92, obesity with an OR of 1.68, and passive smoking with an OR of 1.90, were classified as highly suggestive evidence (Class II). Influenza, with an OR of 3.33, was considered a risk factor with suggestive evidence (Class III). Multivitamin supplementation during pregnancy, with an OR of 0.76, and low maternal vitamin B12, with an OR of 2.41, were categorized as weak evidence (Class IV).Conclusion: We identified four risk factors including hyperthermia, influenza, obesity, and passive smoking as suggestive or highly suggestive evidence for NTDs. Low maternal vitamin B12 was identified as a risk factor for NTDs, supported by weak evidence.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241061"},"PeriodicalIF":1.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ADAR1 plays a protective role in proximal tubular cells under high glucose conditions by attenuating the PI3K/AKT/mTOR signaling pathway. ADAR1 通过抑制 PI3K/AKT/mTOR 信号通路，在高糖条件下对近端肾小管细胞起到保护作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-10 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1037

Ying Wang, Jiang Chang, Fa Wang, Lianying Lai, ShiXu Yang, Yueying Fu, Xingtian Ma, Chuan Yun

{"title":"ADAR1 plays a protective role in proximal tubular cells under high glucose conditions by attenuating the PI3K/AKT/mTOR signaling pathway.","authors":"Ying Wang, Jiang Chang, Fa Wang, Lianying Lai, ShiXu Yang, Yueying Fu, Xingtian Ma, Chuan Yun","doi":"10.1515/med-2024-1037","DOIUrl":"https://doi.org/10.1515/med-2024-1037","url":null,"abstract":"Background: Adenosine deaminases acting on RNA 1 (ADAR1), an RNA editing enzyme, holds a role in cancer, inflammation, and immunity. However, its specific function in the nephropathy and high-glucose-induced human renal tubular epithelial cells (HK-2) injury in diabetic db/db mice is not clear.Methods: This study explored the expression characteristics of ADAR1 in proximal renal tubular cells of diabetic db/db mice, examining its function in the mechanism of high-glucose-induced HK-2 cell injury. Furthermore, it elucidated the molecular mechanism underlying the protective effect of ADAR1, the regulation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/Akt)/mammalian target of the rapamycin (mTOR) signaling. We observed a decrease in ADAR1 expression in proximal tubular cells of diabetic db/db mice, accompanied by an increase in the expression of inflammation-related markers (PI3K/AKT/mTOR).Results: We constructed and validated ADAR1-overexpression plasmids and used an ADAR1 inhibitor (8-azaadenosine) to carry out cell experiments. The upregulation of ADAR1 expression alleviated high-glucose-induced endoplasmic reticulum stress, reduced HK-2 cell apoptosis, and reduced the expression of inflammation-related indicators (PI3K/AKT/mTOR).Conclusion: Taken together, the pivotal roles of ADAR1 in the progression of proximal renal tubulopathy and the mechanism of high-glucose-induced HK-2 injury in diabetic db/db mice suggest that ADAR1 may be a potential key factor in slowing the progression of diabetic kidney disease.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241037"},"PeriodicalIF":1.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11472009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SENP7 inhibits glioblastoma metastasis and invasion by dissociating SUMO2/3 binding to specific target proteins. SENP7 通过分离 SUMO2/3 与特定靶蛋白的结合，抑制胶质母细胞瘤的转移和侵袭。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-07 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1052

Jixing Zhang, Hongshan Zheng, Peng Liang

{"title":"SENP7 inhibits glioblastoma metastasis and invasion by dissociating SUMO2/3 binding to specific target proteins.","authors":"Jixing Zhang, Hongshan Zheng, Peng Liang","doi":"10.1515/med-2024-1052","DOIUrl":"https://doi.org/10.1515/med-2024-1052","url":null,"abstract":"Background: The poor surgical efficacy and recurrence of glioblastoma (GBM) are due to its lack of visible infiltrative features. Our bioinformatics study suggests that low expression of small ubiquitin-like modifier (SUMO)-specific protease 7 (SENP7) indicates poor prognosis in GBM.Objectives: This study investigated the effect of SENP7 expression on the invasion, migration, and proliferation of GBM cells and aims to identify the SUMO target proteins affected by SENP7.Methods: SENP7 expression was analyzed in eight GBM tumor samples and four GBM cell lines, comparing them to normal brain tissue. The effect of SENP7 overexpression on GBM LN229 cell migration, invasion, and proliferation was examined through in vitro assays. Furthermore, four SUMO target proteins involved in tumor invasion and proliferation (CDK6, matrix metalloproteinase-9 [MMP9], AKT, and HIF-1α) were studied to explore SENP7's molecular mechanism.Results: SENP7 expression was significantly lower in GBM tumors compared to normal tissue. SENP7 overexpression in LN229 cells inhibited migration and invasion without affecting proliferation. Overexpression reduced the levels of MMP9, AKT, and HIF-1α, but not CDK6. Immunohistochemical analysis showed decreased MMP9 and CD31 levels, suggesting reduced tumor invasion and angiogenesis. However, SENP7 overexpression did not affect tumor growth in vivo.Conclusions: SENP7 inhibits GBM invasion by dissociating proteins associated with tumor invasion from SUMO2/3, providing a potential target for future GBM therapies.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241052"},"PeriodicalIF":1.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of diagnostic value of unilateral systematic biopsy combined with targeted biopsy in detecting clinically significant prostate cancer. 评估单侧系统活检结合靶向活检在检测有临床意义的前列腺癌方面的诊断价值。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-04 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1048

Jian Wu, Guang Xu, Lihua Xiang, Lehang Guo, Shuai Wang, Lin Dong, Liping Sun

{"title":"Assessment of diagnostic value of unilateral systematic biopsy combined with targeted biopsy in detecting clinically significant prostate cancer.","authors":"Jian Wu, Guang Xu, Lihua Xiang, Lehang Guo, Shuai Wang, Lin Dong, Liping Sun","doi":"10.1515/med-2024-1048","DOIUrl":"https://doi.org/10.1515/med-2024-1048","url":null,"abstract":"Objectives: This retrospective study assessed the diagnostic accuracy of targeted biopsy (TB) and unilateral systematic biopsy in detecting clinically significant prostate cancer (csPCa) in 222 men with single magnetic resonance imaging (MRI) lesions (Prostate Imaging Reporting and Data System [PI-RADS] ≥ 3).Methods: Patients underwent multiparametric MRI and MRI/ultrasound fusion TB and 12-needle standard biopsy (SB) from September 2016 to June 2021. The study compared the diagnostic performance of TB + iSB (ipsilateral), TB + contralateral system biopsy (cSB) (contralateral), and TB alone for csPCa using the χ 2 test and analysis of variance.Results: Among 126 patients with csPCa (ISUP ≥ 2), detection rates for TB + iSB, TB + cSB, and TB were 100, 98.90, and 100% for lesions, respectively. TB + iSB showed the highest sensitivity and negative predictive value. No significant differences in accuracy were found between TB + iSB and the gold standard for type 3 lesions (P = 1). For types 4-5, detection accuracy was comparable across methods (P = 0.314, P = 0.314, P = 0.153). TB had the highest positive needle count rate, with TB + iSB being second for type 3 lesions (4.08% vs 6.57%, P = 0.127).Conclusion: TB + iSB improved csPCa detection rates and reduced biopsy numbers, making it a viable alternative to TB + SB for single MRI lesions.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241048"},"PeriodicalIF":1.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echinacoside inhibits PASMCs calcium overload to prevent hypoxic pulmonary artery remodeling by regulating TRPC1/4/6 and calmodulin. 紫锥菊苷通过调节TRPC1/4/6和钙调素，抑制PASMC钙超载，防止缺氧性肺动脉重塑。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-04 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1044

Enqi Zhao, Jinyu Wang, Yuefu Zhao, Qingqing Xia, Hongmai Wang, Zhanqiang Li, Cen Li, Xiangyun Gai

{"title":"Echinacoside inhibits PASMCs calcium overload to prevent hypoxic pulmonary artery remodeling by regulating TRPC1/4/6 and calmodulin.","authors":"Enqi Zhao, Jinyu Wang, Yuefu Zhao, Qingqing Xia, Hongmai Wang, Zhanqiang Li, Cen Li, Xiangyun Gai","doi":"10.1515/med-2024-1044","DOIUrl":"https://doi.org/10.1515/med-2024-1044","url":null,"abstract":"Abstract: Research indicates that hypoxic pulmonary hypertension (HPH) potentially stimulates the sympathetic nervous system, which may increase norepinephrine (NE) release and cause excessive Ca2+ influx into pulmonary artery smooth muscle cells (PASMCs), leading to calcium overload and abnormal PASMC proliferation, factors closely associated with pulmonary vascular remodeling (PVR). This study investigates the potential mechanisms underlying echinacoside (ECH) treatment in HPH.Method: In the in vitro experiment, NE-induced PASMCs were used to simulate HPH-induced PASMCs' calcium overload and abnormal proliferation. Postincubation with ECH, [Ca2+]cyt changes were detected using Fluo-4 AM. Flow cytometry was employed to ascertain ECH's inhibitory effect on PASMCs proliferation. For in vivo experiments, rats were exposed to a hypoxic and low-pressure oxygen environment to establish the HPH model. Post-ECH treatment, hematoxylin and eosin (HE) staining was conducted to assess PVR, and western blot analysis was used to examine protein expression in the lung tissues of the different groups.Results: ECH was observed to inhibit [Ca2+]cyt increase in NE-induced PASMCs in a concentration-dependent manner, effectively reducing abnormal cell proliferation. It also reduced the expression of Transient receptor potential channel (TRPC) 1 (TRPC1), TRPC4, TRPC6, and calmodulin in PASMCs. In vivo studies demonstrated that ECH lowered the expression of these proteins in lung tissues of HPH rats, significantly decreased mean pulmonary artery pressure, and mitigated PVR.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241044"},"PeriodicalIF":1.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of auto-HSCT in extranodal natural killer/T cell lymphoma. 自体造血干细胞移植在结节外自然杀伤/T细胞淋巴瘤中的作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-03 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1024

Yin-Yin Peng, Xin Wang, Lin Liu

{"title":"The role of auto-HSCT in extranodal natural killer/T cell lymphoma.","authors":"Yin-Yin Peng, Xin Wang, Lin Liu","doi":"10.1515/med-2024-1024","DOIUrl":"https://doi.org/10.1515/med-2024-1024","url":null,"abstract":"Objectives: Autologous hematopoietic stem cell transplantation (auto-HSCT) is considered optional consolidation therapy especially for relapsed/refractory extranodal NK/T-cell lymphoma (ENKL), but its applications to newly diagnosed advanced-stage ENKL is currently limited.Methods: We collected 51 cases of newly diagnosed advanced-stage ENKL patients, including 26 with auto-HSCT and 25 with chemotherapy rather than HSCT, from our hospital between 2014/01 and 2023/12. We summarized the patients' characteristics, conducted survival analysis of the 51 cases, and analyzed the potential benefits of auto-HSCT to ENKL patients.Results: It shows that after a median follow-up time of 39 months, the estimated 5-year overall survival (OS) of the 51 newly diagnosed advanced-stage ENKL patients is 73.4%, and their estimated 5-year progression-free survival (PFS) is 73.4%. For patients receiving auto-HSCT, the 5-year OS (91.7%) and PFS (91.0%) are significantly different from those of patients receiving chemotherapy without HSCT (OS 53.3%, PFS 54.5%) (p < 0.05). Univariate and multivariate analysis results suggest that only the l-asparaginase usage in chemotherapy showed significant impact on the OS, and none of concerned factors showed significant impact on the PFS.Conclusions: Auto-HSCT is indeed an option to newly diagnosed advanced-stage ENKL, but further studies are still required for more strict disease management.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241024"},"PeriodicalIF":1.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-guided transperineal vs transrectal prostate biopsy: A meta-analysis of diagnostic accuracy and complication rates. 超声引导下经会阴与经直肠前列腺活检：诊断准确性和并发症发生率的荟萃分析。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-02 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1039

Tao Wu, Yanchun Xing

{"title":"Ultrasound-guided transperineal vs transrectal prostate biopsy: A meta-analysis of diagnostic accuracy and complication rates.","authors":"Tao Wu, Yanchun Xing","doi":"10.1515/med-2024-1039","DOIUrl":"https://doi.org/10.1515/med-2024-1039","url":null,"abstract":"Objectives: We conducted a systematic review to compare the diagnostic utility of ultrasound-guided transperineal (TP) and transrectal (TR) prostate biopsy methods for prostate cancer detection.Methods: We searched PubMed, Embase, Web of Science, and Cochrane databases up to October 30, 2023, for relevant studies, screening the literature and assessing bias independently.Results: Eleven trials were analyzed using relative risk and 95% confidence intervals, with no evidence of publication bias. Diagnostic rates showed no significant difference between TP and TR biopsies (mean difference [MD]: 1.03, 95% confidence interval [CI]: 0.91-1.14, P = 0.56). Prostate volume analysis also showed no significant difference (MD: -0.07, 95% CI: -0.73 to 0.59, P < 0.0001, combined effect size P = 0.83). Similarly, PSA levels were comparable between TP and TR biopsies (MD: 0.93, 95% CI: -0.44 to 2.30, P < 0.0001, combined effect size P = 0.18).Conclusion: Both biopsy methods exhibit similar diagnostic accuracy; however, TP has a lower risk of biopsy.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241039"},"PeriodicalIF":1.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy. 阴道镜检查诊断宫颈病变时宫颈细胞学阴性和 HPV 阳性的意义。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-10-02 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1051

Changhong Zhang, Liu Dong, Kejie Liu, Hong Xiao, Hao Si, Xiaoqin Wang, Hui Wang

{"title":"Significance of negative cervical cytology and positive HPV in the diagnosis of cervical lesions by colposcopy.","authors":"Changhong Zhang, Liu Dong, Kejie Liu, Hong Xiao, Hao Si, Xiaoqin Wang, Hui Wang","doi":"10.1515/med-2024-1051","DOIUrl":"https://doi.org/10.1515/med-2024-1051","url":null,"abstract":"Objective: The aim of this study was to investigate the significance of colposcopy in diagnosing cervical lesions when negative cervical cytology is combined with positive human papillomavirus (HPV).Methods: Overall, 370 patients with cervical epithelial lesions who had negative cervical fluid-based cytology combined with positive HPV results were selected and analysed for severity of cervical lesions and HPV distribution.Results: Among the patients with cervical lesions, 242 had a single HPV infection, and 128 cases had multiple infections. No significant difference was found between HPV single infection and multiple infections in both groups of patients with cervical lesions (P > 0.05). Furthermore, 137 had non-HPV 16 and 18, accounting for 37.30% of all the patients with cervical lesions. Among them, HPV 52, 58, and 33 infections were the most common at 38.69, 30.66 and 29.20%, respectively - significantly higher than other high-risk HPV types (P < 0.05).Conclusion: High-risk HPV testing is crucial in patients with negative cervical fluid-based cytology combined with positive HPV results. Patients with HPV 16 and 18 and those with simple HPV 52, 58, and 33 infections should undergo timely colposcopy.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241051"},"PeriodicalIF":1.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis. FOXM1 和 AURKB 在调节银屑病角质细胞功能中的作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1049

Zhaofeng Zhao, Jie Cheng, Qiang Hou, Jian Zhu, Tu Chen, Sheng Lu, Guiju Wu, Hongli Lv, Xiujuan Wu

{"title":"Role of FOXM1 and AURKB in regulating keratinocyte function in psoriasis.","authors":"Zhaofeng Zhao, Jie Cheng, Qiang Hou, Jian Zhu, Tu Chen, Sheng Lu, Guiju Wu, Hongli Lv, Xiujuan Wu","doi":"10.1515/med-2024-1049","DOIUrl":"https://doi.org/10.1515/med-2024-1049","url":null,"abstract":"Objective: This study investigated the effect of forkhead box M1 (FOXM1) and Aurora kinase B (AURKB) on the epidermal function of keratinocytes.Methods: Bioinformatics analysis was used to analyze the co-expression network of FOXM1 and its correlation with AURKB. The expression of FOXM1 and AURKB in tissues and cells was detected by immunofluorescence and real-time quantitative polymerase chain reaction, respectively. HaCaT cells were transfected with si-FOXM1 to knock down FOXM1. Cell proliferation was detected by cell counting kit-8 assay. Cell migration was detected by scratch assay. Cell invasion was detected by the Transwell invasion assay. Cell apoptosis and cell cycle were detected by flow cytometry.Results: FOXM1 and AURKB were positively correlated and highly expressed in psoriatic lesions. After transfection of si-FOXM1, the expression levels of FOXM1 and AURKB genes significantly decreased. The proliferation of HaCaT cells decreased, the apoptosis rate increased significantly, and the proportion of cells in the G1 phase increased significantly, while the proportion of cells in the S phase decreased significantly. The scratch closure of HaCaT cells was reduced, and the number of cell invasions decreased significantly.Conclusion: FOXM1 and AURKB may affect the progression of psoriasis by regulating the proliferation, cell cycle, migration, and invasion of keratinocytes.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241049"},"PeriodicalIF":1.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parental control attitudes over their pre-school children's diet. 家长对学龄前儿童饮食的控制态度。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1054

Dulce Ivone Pinto Alves, Moniky Araújo da Cruz, Nadirlene Pereira Gomes, Amâncio António de Sousa Carvalho

{"title":"Parental control attitudes over their pre-school children's diet.","authors":"Dulce Ivone Pinto Alves, Moniky Araújo da Cruz, Nadirlene Pereira Gomes, Amâncio António de Sousa Carvalho","doi":"10.1515/med-2024-1054","DOIUrl":"https://doi.org/10.1515/med-2024-1054","url":null,"abstract":"It is during childhood that eating behaviors begin to form, with parents being the main agents in this process. Parents have eating habits that shape their children's diet, both in terms of variety and quantity of food eaten. The aim is to analyze sociodemographic factors related to parental control over their children's diet. Descriptive-correlational and cross-sectional study, with a sample of 46 parents of preschool children. An online questionnaire was used to collect data, with data processing carried out using SPSS, using descriptive and inferential statistics. The majority of respondents were mothers (89.1%), belonged to the 20-44 age group (89.1%), and were married (89.1%). The mean of the subscales of the children's food questionnaire food restriction, pressure to eat, and monitoring was 3.266 ± 0.570, 3.109 ± 1.206, and 4.268 ± 0.848, respectively. The mean rank score for the food restriction subscale differed significantly between parents with different age groups (Mann-Whitney: p < 0.014), with the 45-64 age group having the highest mean rank, i.e., they restricted their children more in food. The age group is a factor related to food restriction, making it essential to take a closer look at the parents of that age group, during the health education process.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241054"},"PeriodicalIF":1.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0