Ontogenez最新文献_第3页

[Efficiency of the Induction of Cytomixis in the Microsporogenesis of Dicotyledonous (N. tabacum L.) and Monocotyledonous(H. distichum L.) Plants by Thermal Stress]. [通过热胁迫在双子叶植物（N.tabacum L.）和单子叶植物（H.distichum L.）的小孢子发生中诱导细胞融合的效率]。

Ontogenez Pub Date : 2016-11-01

Yu V Sidorchuk, E A Kravets, S R Mursalimov, S G Plokhovskaya, I I Goryunova, A I Yemets, Y B Blume, E V Deineko

{"title":"[Efficiency of the Induction of Cytomixis in the Microsporogenesis of Dicotyledonous (N. tabacum L.) and Monocotyledonous\u0000(H. distichum L.) Plants by Thermal Stress].","authors":"Yu V Sidorchuk, E A Kravets, S R Mursalimov, S G Plokhovskaya, I I Goryunova, A I Yemets, Y B Blume, E V Deineko","doi":"","DOIUrl":"","url":null,"abstract":"The efficiencies of the induction of cytomixis in microsporogenesis by thermal stress are compared\u0000in tobacco (N. tabacum L.) and barley (H. distichum L.) It has been shown that different thermal treatment\u0000schedules (budding tobacco plants at 50°C and air-dried barley grains at 48°C) produce similar results in the\u0000species: the frequency of cytomixis increases, and its maximum shifts to later stages of meiosis. However, the\u0000species show differences in response. The cytomixis frequency increase in tobacco is more pronounced, and\u0000its maximum shifts from the zygotene–pachytene stages of meiotic prophase I to prometaphase–metaphase\u0000I. Later in the meiosis, aberrations in chromosome structure and meiotic apparatus formation typical of cytomixis\u0000are noted, as well as cytomixis activation in tapetum cells. Thermal stress disturbs the integration of callose-\u0000bearing vesicles into the callose wall. Cold treatment at 7°C does not affect cytomixis frequency in\u0000tobacco microsporogenesis. Incubation of barley seeds at 48°C activates cytomixis in comparison to the control,\u0000shifts its maximum from the premeiotic interphase to zygotene, and changes the habit of cytomictic\u0000interactions from pairwise contacts to the formation of multicellular clusters. Thermal treatment induces\u0000cytomictic interactions within the tapetum and between microsporocytes and the tapetum. However, later\u0000meiotic phases show no adverse consequences of active cytomixis in barley. It is conjectured that heat stress\u0000affects callose metabolism and integration into the forming callose wall, thereby causing incomplete closure\u0000of cytomictic channels and favoring intercellular chromosome migration at advanced meiotic stages.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 6","pages":"357-72"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36537025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Features of Clonal Micropropagation of Silene cretacea (Caryophyllaceae) in in vitro Culture]. [石竹科(Silene白垩纪)无性系离体培养繁殖特征]。

Ontogenez Pub Date : 2016-11-01

T A Kritskaya, A S Kashin, V A Spivak, V E Firstov

引用次数: 0

[Position of the Nucleus in Mouse Germinal Vesicle–Stage Oocytes with Different Chromatin Configurations]. [不同染色质结构的小鼠胚泡期卵母细胞细胞核的位置]。

Ontogenez Pub Date : 2016-11-01

K V Shishova, E A Lavrentyeva, A I Khamidullina, O V Zatsepina

{"title":"[Position of the Nucleus in Mouse Germinal Vesicle–Stage Oocytes with Different Chromatin Configurations].","authors":"K V Shishova, E A Lavrentyeva, A I Khamidullina, O V Zatsepina","doi":"","DOIUrl":"","url":null,"abstract":"The mammalian germinal vesicle–stage (GV) oocytes are divided into two major types, NSN\u0000(non-surrounded nucleolus) and SN (surrounded nucleolus), and at least one intermediate type, pSN (partly\u0000surrounded nucleolus), based on large-scale chromatin configuration. In mice, the SN oocytes are considered\u0000to be the most meiotically competent, which explains active study of their phenotypic characteristics\u0000necessary for improvement of human reproductive technologies. One of such characteristics is the position\u0000of the GV (nucleus) relative to the center of the oocyte. However, the current data on this issue are contradictory\u0000and even completely absent for pSN oocytes. In this work, we have studied the GV position in 187\u0000mouse GV oocytes belonging to NSN, SN, and pSN types using different approaches known from the literature.\u0000Our results suggest that (1) the most abundant in all examined types of oocytes are central GVs (43–\u000066%) and the least abundant are peripheral GVs (12–39%); the pSN oocytes are closer to SN oocytes rather\u0000than to NSN oocytes according to the GV position; (3) the position of the nucleus in mouse GV oocytes is\u0000an ambiguous marker of large-scale chromatin configuration and, correspondingly, maturation competence\u0000of the oocyte; (4) the diversity of the GV position in NSN, SN, and pSN oocytes most likely reflects the ability\u0000of GVs to migrate; and (5) assessment of the GV position according to three variants (central, peripheral,\u0000and intermediate) is more informative as compared with two variants (central and peripheral).","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 6","pages":"331-8"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36539425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Histological and Immunocytochemical Investigation of Human Coronary Vessel Development with ANTI-CD34 Antibodies]. 抗cd34抗体对人冠状血管发育的组织学和免疫细胞化学研究

Ontogenez Pub Date : 2016-11-01

O Yu Pototska

{"title":"[Histological and Immunocytochemical Investigation of Human Coronary Vessel Development with ANTI-CD34 Antibodies].","authors":"O Yu Pototska","doi":"","DOIUrl":"","url":null,"abstract":"Information about embryonic development of coronary endothelium is the main clue for the creation\u0000of new methods in tissue engineering for treatment of ischemic heart diseases. The purpose of the\u0000research was to describe human coronary vessels development on early stages of the prenatal ontogenesis. The\u0000first step in human coronary vessels development is the formation of endothelium de novo by transformation\u0000of some epicardial and, possibly, endocardial cells. The next step is the ingrowth of sinus venosus endothelium\u0000in subepicardium over ventricles and atria, which gives rise to the coronary vessels. Only after 7 days does\u0000the primitive coronary plexus of the heart communicate with aorta (third step). During this period, some subepicardial\u0000vessels invade myocardium and some intramyocardial vessels contact with the heart cavity. Such\u0000intercommunications could help in regulation of blood circulation in primitive coronary plexus before establishment\u0000of effective contacts between arterial and venous vessels—excess of blood could be discharged\u0000directly into the heart cavity. Additional population of CD34+ cells were revealed inside condensed mesenchyme\u0000of the conotruncus; it participates in the formation of vasa vasorum in the aorta. Epicardium and sinus\u0000venosus generate endothelium of coronary vessels by neovasculo- and angiogenesis, respectively. During a\u0000week after ingrowth of vessels from SV and before their ingrowth to the aorta, ventriculo-coronary communications\u0000could be found in the heart.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 6","pages":"373-85"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36537032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Hormone-Induced In Vitro Maturation and Ovulation of Danio rerio Oocytes and Production of Eggs Capableof Fertilization and Futher Development]. [激素诱导的斑马鱼卵母细胞体外成熟和排卵及能够受精和进一步发育的卵子的产生]。

Ontogenez Pub Date : 2016-09-01

M N Skoblina, A A Minin

引用次数: 0

[Reciprocal Humoral Regulation of Endocrine Noradrenaline Sources in Perinatal Development of Rats]. [大鼠围产期发育中去甲肾上腺素源内分泌的体液相互调节]。

Ontogenez Pub Date : 2016-09-01

Y O Nikishina, A R Murtazina, A Ya Sapronova, V I Melnikova, N S Bondarenko, M V Ugryumov

{"title":"[Reciprocal Humoral Regulation of Endocrine Noradrenaline Sources in Perinatal Development of Rats].","authors":"Y O Nikishina, A R Murtazina, A Ya Sapronova, V I Melnikova, N S Bondarenko, M V Ugryumov","doi":"","DOIUrl":"","url":null,"abstract":"The goal of the present study was to verify our hypothesis of humoral interaction between the norepinephrine\u0000secreting organs in the perinatal period of ontogenesis that is aimed at the sustaining of physiologically\u0000active concentration of norepinephrine in blood. The objects of the study were the transitory organs,\u0000such as brain, organ of Zuckerkandl, and adrenals, the permanent endocrine organ of rats that releases norepinephrine\u0000into the bloodstream. To reach this goal, we assessed the adrenal secretory activity (norepinephrine\u0000level) and activity of the Zuckerkandl’s organ under the conditions of destructed noradrenergic neurons of brain\u0000caused by (1) their selective death induced by introduction of a hybrid molecular complex, which consisted of\u0000antibodies against dopamine-β-hydroxylase (DBH) conjugated with saporin cytotoxin (anti-DBH-saporin)\u0000into the lateral brain ventricles of neonatal rats; and (2) microsurgical in utero destruction of embryo’s brain\u0000(in utero encephalectomy). It was observed that 72 h after either pharmacological or microsurgical norepinephrine\u0000synthesis deprivation in the newborn rat’s brain, the level of norepinephrine was increased in\u0000adrenals and, conversely, decreased in the Zuckerkandl’s organ. Therefore, the experiments with models of\u0000chronical inhibition of norepinephrine synthesis in prenatal and early postnatal rat’s brain revealed changes\u0000in the secretory activity of peripheral norepinephrine sources. This, apparently, favors the sustaining of physiologically\u0000active norepinephrine level in the bloodstream.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 5","pages":"287-95"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36539779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Individual Growth of the Great Ramshorn Snail Planorbarius corneus (Gastropoda, Planorbidae) Embryos]. [大蓝角蜗牛(腹足纲，腹足科)胚胎的个体生长]。

Ontogenez Pub Date : 2016-09-01

A A Zotin, E F Kirik

{"title":"[Individual Growth of the Great Ramshorn Snail Planorbarius corneus (Gastropoda, Planorbidae) Embryos].","authors":"A A Zotin, E F Kirik","doi":"","DOIUrl":"","url":null,"abstract":"Individual growth of the great ramshorn snail Planorbarius corneus has been studied by intravital\u0000video imaging. As has been observed, the types of growth change over the embryogenesis. The linear dimensions\u0000slightly but in a statistically significant manner decrease during the stages of cleavage to blastula. Starting\u0000from the stage of blastula to trochophore, the embryo diameter remains constant to commence increasing\u0000at the stage of middle trochophore. During the larval stages (trochophore and veliger), the growth is synchronous\u0000(in Dettlaffs, biological time units) for the embryos in both the same clutch and different clutches. The\u0000growth at that time is exponential but later desynchronizes in individual clutches. The embryos in eight\u0000clutches grew and developed slower and hatched later as compared with the remaining five egg clutches. An\u0000accelerated growth follows an asymptomatic pattern according to the von Bertalanffy equation. A retarded\u0000growth is describable with a linear equation. The observed differences are likely to be associated with the\u0000number of embryos in a clutch. All types of changes in the linear dimensions observed in the great ramshorn\u0000snail embryogenesis can be described with the same united equation.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 5","pages":"296-306"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36539778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Gene Network Controlling the Morphogenesis of D. melanogaster Macrochaetes: An Expanded Model of the Central Regulatory Circuit]. [D. melanogaster macrochetes形态发生的基因网络:中央调控回路的扩展模型]。

Ontogenez Pub Date : 2016-09-01

T A Bukharina, V P Golubyatnikov, D P Furman

{"title":"[Gene Network Controlling the Morphogenesis of D. melanogaster Macrochaetes: An Expanded Model of the Central Regulatory Circuit].","authors":"T A Bukharina, V P Golubyatnikov, D P Furman","doi":"","DOIUrl":"","url":null,"abstract":"The drosophila macrochaetes act as mechanoreceptors, the sensory organs of the peripheral nervous\u0000system. Each mechanoreceptor consists of four specialized cells, namely, the shaft, socket, neuron, and\u0000sheath. All these cells develop from a single cell referred to as the sensory organ precursor (SOP) cell. The\u0000SOP cell segregates from the surrounding cells of imaginal disc, thereby launching multistage sensory organ\u0000development. A characteristic feature of the SOP cell is the highest content of the proneural proteins Achaete\u0000and Scute (ASC) as compared with the surrounding cells. The pattern of changes in the content of proneural\u0000proteins in the SOP cell is determined by a gene network with the achaete-scute (AS-C) gene complex as its\u0000key component. The activity of this complex is controlled by the central regulatory circuit (CRC), containing\u0000the genes hairy, senseless (sens), charlatan (chn), scratch (scrt), daughterless (da), extramacrochaete (emc), and\u0000groucho (gro), encoding the transcription factors involved in the system of feedforwards and feedbacks and\u0000implementing the activation–repression of CRC components, as well as the gene phyllopod (phyl), an adaptor\u0000protein that controls the degradation of ASC proteins. A mathematical model describing the CRC functioning\u0000in the SOP cell as a regulator of the content of ASC proneural proteins is proposed.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 5","pages":"307-13"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36539780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Genetically Determined and Functional Human Sperm Motility Decrease]. [基因决定和功能性人类精子活力下降]。

Ontogenez Pub Date : 2016-09-01

E E Bragina, E A Arifulin, E P Senchenkov

{"title":"[Genetically Determined and Functional Human Sperm Motility Decrease].","authors":"E E Bragina, E A Arifulin, E P Senchenkov","doi":"","DOIUrl":"","url":null,"abstract":"Motility is the most important property of mammalian sperm required for fertilization. Axoneme\u0000and axoneme surrounding tail components are the morphological substrate of sperm motility. Quantitative\u0000research methods of human spermatozoa motility allowed the definition of the normative parameters for fertile\u0000men. Exogenous factors, and, rarely, genetic defects may cause a significant reduction in sperm motility.\u0000Axonemal anomalies (absence of external and/or internal dynein arms, central pair of microtubules absence)\u0000may be the cause of primary ciliary dyskinesia (PCD). PCD—a severe systemic disease of the reduction of\u0000sperm motility—is just one symptom. Dysplasia of the fibrous sheath (DFO) is also genetically determined\u0000sperm motility decrease. PCD and the DFO are multigene diseases that are inherited in an autosomal recessive\u0000manner. Modern molecular biological research methods are used to identify candidate genes. Assisted\u0000reproduction technologies (ART) allow men suffering from PCD and DFO to produce offspring. PCD and\u0000DFO symptoms appear in the homozygote. Children born after ART have the probability of being mutation\u0000carriers. We do not have complete information about etiological factor of genetically determined spermpathology.\u0000So we cannot assess the genetic risk degree. However, the possibility of mutations accumulation,\u0000which can be a risk factor for distant offspring, should be considered.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"47 5","pages":"271-86"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36536521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Specificity of the Hemodynamic Indices’ Shift in SHR Line Rats at Different Age]. [不同年龄SHR系大鼠血流动力学指标变化的特异性]。

Ontogenez Pub Date : 2016-09-01

A Yu Shamanaev, O I Aliev, A M Anishchenko, A V Sidekhmenova, M B Plotnikov

引用次数: 0