Open Veterinary Journal最新文献

{"title":"Molecular, epidemiological, and hematological evaluation in <i>Ehrlichia canis</i> infected dogs from an endemic region in Egypt.","authors":"Dina A Mobarak, Elzahara K Elbaz, Samar M Atwa, Mohamed I Eisa, Ahmed M El-Sebaey, Ahmed M Selim","doi":"10.5455/OVJ.2024.v14.i8.10","DOIUrl":"10.5455/OVJ.2024.v14.i8.10","url":null,"abstract":"<p><strong>Background: </strong>Canine monocytic ehrlichiosis (CME) is considered a multisystemic, life-threatening, rickettsial, and tick-borne disease that affects canine species and is caused by <i>Ehrlichia canis (E. canis)</i>. Clinical signs of CME vary from asymptomatic to severe illness with three clinical phases. <i>E. canis</i> has the potential to infect humans.</p><p><strong>Aim: </strong>This study aimed to provide recent information as there is limited data about the disease in Egypt. Therefore, this work was conducted to study the molecular prevalence of <i>E. canis</i> and evaluate the corresponding risk factors, hematology, biochemistry, and molecular characterization of the genus Ehrlichia and <i>E. canis</i> species among Egyptian dogs.</p><p><strong>Methods: </strong>One hundred eighty dogs of both sexes from 3 months to 8 years from different breeds: stray and foreign breeds were examined for clinical signs in all seasons in two delta governorates: El-Dakahlia and El-Gharbia. Blood samples were collected from dogs for microscopic and haemato-biochemical analysis, and then molecular characterization of the genus Ehrlichia and species-specific <i>E. canis</i> was performed, followed by sequencing and phylogenetic analysis.</p><p><strong>Results: </strong>Out of 180 samples examined by polymerase chain reaction (PCR) assay, 42 (23.33%) were positive for the genus of Ehrlichia and the species-specific <i>E. canis.</i> Only twenty-four dogs (13.33%) were positive for PCR, infested with ticks, and showed fever, anemia, loss of body weight, pale mucous membrane of gum and conjunctiva, blindness, paralysis, hemoglobinuria, and Melena. The univariate logistic regression revealed that all variables, including age, season, tick infestation, hemorrhage from natural orifices, and ectoparasitic treatments per year, showed statistical significance (<i>p</i> ≤ 0.05), except breed and sex, which also did not exhibit any relation between CME infection in multivariate logistic regression. The presence of morulae inside leukocytes in 66 dogs out of the total examined 180 (36.67%), only 39 (59.1%) were positive for morulae and PCR-positive for <i>E. canis</i>. Dogs positive for <i>E. canis</i> suffered from anemia, severe thrombocytopenia, the absolute value of WBCs and their fractions, alanine aminotransferas (ALT), AST, ALKP, γ-GT, total. P, T.BIL, urea, globulin, and creatinine were significantly increased in dogs infected with <i>E. canis</i> when compared to those with negative PCR results, while the levels of albumin and A: G ratios were significantly decreased.</p><p><strong>Conclusion: </strong>The current study proves the existence of <i>E. canis</i> in El-Dakahlia and El-Gharbia governorates, and this is the first large-scale study concerning the epidemiological, clinicopathological examination, molecular characterization, sequencing, and phylogenetic analysis of reported from the center of the Delta of the Nile in Egypt.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1819-1835"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring of bone matrix acidification by TRAP and ERK biomarkers in the chronic hypercholesterolemia male rats.","authors":"Duaa Raad Abd-Al-Ameer, Wefak Albazi, Hayder Ali Muhammed","doi":"10.5455/OVJ.2024.v14.i8.11","DOIUrl":"10.5455/OVJ.2024.v14.i8.11","url":null,"abstract":"<p><strong>Background: </strong>Hypercholesterolemia is frequently linked to an elevated risk of cardiovascular diseases, including heart attacks and strokes. Additionally, it could be connected to a higher susceptibility to osteoporosis. Hypercholesterolemia can stimulate the differentiation and activity of osteoclasts, leading to enhanced bone reabsorption and a subsequent net loss of bone tissue.</p><p><strong>Aim: </strong>The purpose of this study was to examine the influence of a high-cholesterol diet on osteoporosis in male rats with differences in biological and oxidative indicators in the hypercholesterolemia diet in male rats.</p><p><strong>Methods: </strong>The samples in this study were twenty male rats, ranging between 1.5 and 2 months, were separated into two groups. In one group, 10 rats were fed a regular diet, while in another group, 10 rats were fed a high-cholesterol diet (2%) over the course of 8 weeks. Samples of blood were obtained at the last stage of the experiment. To calculate physiological and biological markers including extracellular signal-regulated kinase (ERK), tartrate-resistant acid phosphatase (TRAP), hormones, malondialdehyde (MDA), and glutathione (GSH).</p><p><strong>Results: </strong>The results of this study demonstrated a decrease in GSH levels, an increase in ERKs, no significant change in serum TRAP levels, an increase in MDA levels in the blood, and elevated levels of parathyroid hormone, calcitonin, and vitamin D in the cholesterol group.</p><p><strong>Conclusion: </strong>Increased oxidative stress, altered signaling, and disruptions in calcium/bone metabolism associated with cholesterol-related conditions and monitoring biomarker ERK can provide valuable information about disease progression.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1836-1842"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal abscess in a Lionhead rabbit due to <i>Encephalitozoon cuniculi</i> and <i>Escherichia coli</i>: A case report.","authors":"Warisraporn Tangchang, Sang-Hun Kim, Su-Young Park, Eun-Hye Jung, Hyo-Jung Kwon, Hwa-Young Son","doi":"10.5455/OVJ.2024.v14.i8.38","DOIUrl":"10.5455/OVJ.2024.v14.i8.38","url":null,"abstract":"<p><strong>Background: </strong>In rabbits, renal abscesses (pus-filled sores) are rare and diagnosis remains challenging. Therefore, in this study, we aimed to determine the clinical manifestation and diagnostic tests associated with renal abscess identification in rabbits.</p><p><strong>Case description: </strong>A four-and-a-half-year-old castrated male Lionhead rabbit with a history of poor appetite and abdominal distension was admitted to the animal hospital. Blood analysis, radiography, ultrasonography, and computed tomography scans revealed a kidney abscess found within the renal parenchyma, with severe loss of the cortex and medulla, extending toward the capsule. Consequently, the rabbit underwent nephrectomy. The enlarged right kidney was surgically removed. Histopathological examination of the affected kidney showed severe necrosis and ischemic zones, atrophy of the renal tubules, and prominent heterophils with mixed inflammatory cell infiltrates. Immunohistochemistry and polymerase chain reaction confirmed <i>Encephalitozoon cuniculi</i> and <i>Escherichia coli</i> infections, respectively.</p><p><strong>Conclusion: </strong>This report provides novel insights into the diagnosis of renal abscesses in Lionhead rabbits.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"2085-2091"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sudden death due to enterotoxemia among Arabian camels (<i>Camelus dromedaries</i>) and associated risk factors.","authors":"Asmaa G Mubarak, Fatma A Khalifa, Yumna Elsobky, Ahmed Abdel-Rady, Wael Felefel, Adel Hassan Saad, Ehab Y Abdelhiee, Abdullah M Alhassan, Hisham Awny, Eman M Elghazaly, Ashraf M Abu-Seida, Abdulrahman Abdulkarim, Asmaa G Youseef","doi":"10.5455/OVJ.2024.v14.i8.23","DOIUrl":"10.5455/OVJ.2024.v14.i8.23","url":null,"abstract":"<p><strong>Background: </strong>Sudden death is defined as an unexpected death occurring with no observed antecedent clinical signs.</p><p><strong>Aim: </strong>The current study was performed to notice the tangible causes of sudden death among 51 out of 340 she-camels on a private farm in the eastern region of El Khafgi, Saudi Arabia.</p><p><strong>Methods: </strong>A retrospective cohort study design was conducted to investigate the sudden death of camels through microscopic examination of fecal matter to identify the gastrointestinal parasites, analysis of whole blood thin films to diagnose blood parasites, blood culturing to recognize bacterial infection as <i>Pasteurella multicida</i>, and macroscopic postmortem examination to identify the gastrointestinal adult worm. The quantity and composition of feed were also analyzed. Afterward, a commercial multiscreen Ag-ELISA kit technique determined the toxins of <i>Clostridium perfringens</i> (<i>C. perfringens</i>).</p><p><strong>Results: </strong>The results revealed that the incidence rate of sudden death was 15%. The sudden death occurred due to <i>C. perfringens</i> enterotoxins detected in the rumen, intestinal content, and intestinal wall. The enterotoxins and Alpha toxins were noticed, but the other toxin types, including Beta and Epsilon, could not be detected. All <i>C. perfringens</i> toxins were discovered to be negative in fecal matter. A significant association was reported between sudden death, she-camels age, and feeding habits as risk factors (<i>p</i> = 0.020 and 0.028, respectively). Risk factor assessment by relative risk (RR) revealed that the odds of RR of sudden death occurring among she-camels aged over two years were higher than those less than two years (2.24 CI 95%, 1.093-4.591). Furthermore, the odds RR of sudden death occurring due to exposure of she-camels to a concentrated ration of 18% were higher twice than those not exposed (2.346 CI 95%, 1.039-5.296).</p><p><strong>Conclusion: </strong><i>Clostridium perfringens</i> enterotoxaemia should be listed as a cause of sudden death in camels and the alteration in diet with 18% concentration feed changes the intestinal environment, which leads to <i>C. perfringens</i> proliferating and yielding potent toxins. More observations and interferences like regular immunization are recommended to reduce the disease and increase the awareness of the farmers of the importance of risk factors.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1942-1951"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic evaluation of thoracic and abdominal neoplasia in domestic ruminants: A systemic review.","authors":"Mohamed Tharwat, El-Sayed El-Shafaey, Abdulrahman A Alkheraif","doi":"10.5455/OVJ.2024.v14.i8.2","DOIUrl":"10.5455/OVJ.2024.v14.i8.2","url":null,"abstract":"<p><p>There is a lack of literature on the usefulness of ultrasonography in the diagnosis and prognosis of thoracic and abdominal neoplasia in domestic ruminants, such as cattle, camels, sheep, and goats. This review aims to shed light on the current applications and prospects of ultrasonography in the diagnosis of thoracic and abdominal neoplasia in domestic ruminants. The scientific literature on ultrasonographic evaluation of the thoracic and abdominal neoplasia in domestic ruminants has been systematically reviewed to verify the potential role of ultrasonography in diagnosing such neoplasia. Based on the literature results, cattle (71.03%) were the most affected animals by thoracic and abdominal neoplasia, followed by sheep (11.86%), goats (11.57%), and camels (5.54%). In all included species, the abdominal neoplasia was more frequent (6.18%) than the thoracic neoplasia (2.97%), and the most frequent neoplasms were forestomach neoplasia. It is concluded that ultrasonography is not widely used in diagnosing thoracic and abdominal neoplasia in ruminant practice. Using ultrasonography in conjunction with guided needle aspiration and biopsy can offer veterinarians the opportunity for more precise diagnosis and treatment decisions guidance of thoracic and abdominal neoplasia in domesticated ruminants.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1751-1760"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin.","authors":"Herawati Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, Dyah Ayu O A Pratama","doi":"10.5455/OVJ.2024.v14.i8.3","DOIUrl":"10.5455/OVJ.2024.v14.i8.3","url":null,"abstract":"<p><strong>Background: </strong>Diabetes is a degenerative disease associated with metabolic disorders. The majority of people have type 2 diabetes mellitus (DM) insulin resistance due to an unhealthy lifestyle. The development of DM treatment is also growing, one of which is using conditioned medium.</p><p><strong>Aim: </strong>This study aims to determine the effect of Bovine umbilical mesenchymal stem cell-conditioned medium (BUMSC-CM) on nicotinamide (NA) and streptozotocin (STZ) induced rats as an animal model of DM.</p><p><strong>Methods: </strong>The study began with the <i>in vitro</i> docking of Cholecalciferol with aldolase reductase and glucokinase. In the <i>in vivo</i> study, animal models were divided into five groups: group A (negative control), group B (diabetic rats), group C (NA+STZ+Metformin), group D (NA+STZ+ BUMSC-CM 0.2 ml/kg BW), and group E (NA+STZ+ BUMSC-CM 0.5 ml/kg BW). Blood sugar levels were checked, and BUMSC-CM was administered by intramuscular injection at four-day intervals for a duration of 16 days. Blood sugar levels were also sampled, and GLUT4 histochemical and immunohistochemical staining was performed.</p><p><strong>Results: </strong>The results showed that Cholecalciferol can bind to aldolase reductase ASP43 and TYR48 and bind to glucokinase at TYR214 with hydrogen bonds. BUMSC-CM administration was able to reduce blood sugar well. In addition, BUMSC-CM also helped repair the tissue structure of the pancreas damaged by inflammation from STZ administration.</p><p><strong>Conclusion: </strong>This study can be concluded that the administration of BUMSC-CM can be an alternative cell-free therapy for patients with DM.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1761-1770"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Ginkgo biloba</i> administration to apoptosis in rat (<i>Rattus novergicus</i>) brain cells exposed to Plumbum.","authors":"Muhammad Hamdan, Widjiati Widjiati, Priya Nugraha, Jovian Philip Swatan","doi":"10.5455/OVJ.2024.v14.i8.34","DOIUrl":"10.5455/OVJ.2024.v14.i8.34","url":null,"abstract":"<p><strong>Background: </strong>Lead (Plumbum/Pb) has been identified as a potential cause of Parkinson's disease as well as possibly contributing to disease progression. <i>Ginkgo biloba</i> extract has been widely used to prevent and treat stroke which prevents brain cell apoptosis and neuroinflammation. This has been shown to be beneficial in cognitive recovery in stroke incidents.</p><p><strong>Aim: </strong>This study aimed to determine the effect of <i>G. biloba</i> on the expression of superoxide dismutase (SOD), malondialdehyde (MDA), and apoptosis-inducing factor (AIF) in the brain cells of rats (<i>Rattus novergicus</i>) exposed to Plumbum.</p><p><strong>Methods: </strong>The experimental animals used were 36 male white rats divided into 4 groups with different treatments of Plumbum and <i>G. biloba</i> at varying doses for 42 days, after which the brains were collected for examination of SOD, MDA, and AIF expressions using immunohistochemical methods and analyzed using analysis of variance and Duncan's test.</p><p><strong>Results: </strong>Plumbum administration caused a significant decrease in SOD expression and an increase in MDA and AIF expressions (<i>p</i> < 0.05). <i>Ginkgo biloba</i> administration significantly increased SOD expression and decreased MDA and AIF expressions (<i>p</i> < 0.05), with optimal increases in SOD, decreases in MDA, and modulation of AIF observed in the group exposed to 50 mg/kg BW Pb and 300 mg/kg BW <i>G. biloba</i>.</p><p><strong>Conclusion: </strong>Preventive administration of <i>G biloba</i> increased SOD expression, and reduced MDA and AIF expressions in Pb-exposed rats, with an optimal dose of 300 mg/kg BW, suggesting its potential as an affordable drug to prevent brain cell death-related diseases.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"2049-2056"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of GnRH treatment as a potential solution for ovarian disorders in dairy cows infected with foot and mouth disease in Indonesian smallholder farms.","authors":"Joko Susilo, Erif Maha Nugraha Setyawan, Slamet Hartanto, Michael Haryadi Wibowo, Agung Budiyanto","doi":"10.5455/OVJ.2024.v14.i8.37","DOIUrl":"10.5455/OVJ.2024.v14.i8.37","url":null,"abstract":"<p><strong>Background: </strong>The outbreak of foot and mouth disease (FMD) in Indonesia induces reproductive disorders in dairy cows that lead to economic losses to smallholder dairy farms.</p><p><strong>Aim: </strong>The study was to assess the influence of FMD on reproductive traits and evaluate the effect of gonadotropin hormone-releasing hormone (GnRH) administrations on the reproductive performance in FMD-infected dairy cows.</p><p><strong>Methods: </strong>The study was conducted in Jemowo village, Taman Sari sub-district, Boyolali district, Central Java, Indonesia. A total of 155 cows were used to identify the reproductive disorders on FMD-infected dairy cows aged 2-10 years old. Cows were raised in similar conditions and fed diets. A single dose of 2 ml GnRH was injected intramuscularly into 96 ovarian disorder cows. Reproductive performance was measured by service per conception (S/C), conception rate (CR), and pregnancy rate (PR). A descriptive study was conducted to demonstrate the results.</p><p><strong>Results: </strong>The study showed that 61.9% of FMD-infected cows had reproductive disorders, whereby 53.5% ovarian hypofunction, 4.52% silent heat, 1.94% repeat breeder, 1.29% ovarian atrophy, and 0.65% endometritis. FMD-infected cows injected with GnRH had a 98% reproductive recovery rate. Moreover, the S/C, CR, and PR of cows injected with GnRH were 2.02%, 51%, and 85%.</p><p><strong>Conclusion: </strong>GnRH administrations enhanced the reproductive traits of FMD-infected dairy cows indicated by the improvement of CR and PR.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"2079-2084"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metazoan ectoparasites of <i>Lithognathus mormyrus</i> (Linnaeus, 1758) from the western coast of Libya.","authors":"Malak T Altikbali, Mohamed L Showehdi, Sarah A Benzeglam, Aisha A Seif-Alnaser, Esmail A Shakman","doi":"10.5455/OVJ.2024.v14.i8.4","DOIUrl":"10.5455/OVJ.2024.v14.i8.4","url":null,"abstract":"<p><strong>Background: </strong><i>Lithognathus mormyrus</i> fish is one of the fish of high economic importance in the countries of the world in general and the Mediterranean countries in specific, including the Libyan Sea waters, for this it is necessary to know everything related to this type of fish, including its infection with parasites. Parasites are considered one of the factors threatening the life of fish, being one of the food chains and the ecological composition of life.</p><p><strong>Aim: </strong>The current study aims to isolate and identify the parasites that infect the <i>L. mormyrus</i>.</p><p><strong>Methods: </strong>A total of 368 specimens of <i>L. mormyrus</i> were collected by fishermen, from the western coast of Libya. The study was focused on metazoan parasites. When fish were fully examined for the presence of ectoparasites under a dissecting microscope with incident light, the software camera connected with a microscope and semichon' acetocarmine technique for identification of parasites.</p><p><strong>Results: </strong>Two species of Monogenea (<i>Encotyllabe</i> <i>valley, Pagellicotyle mormyri,</i> and <i>Lamellodiscus</i> spp<i>.</i>), Isopoda (Gnathia), Copepoda (<i>Lernaeolophus</i> <i>sultanus</i>), and Annelidae (<i>Trachelobdella lubrica</i>) has been isolated from this species of fish. The highest prevalence of infection was Gnathia parasites (8.47%).</p><p><strong>Conclusion: </strong>There were differences in the parasite species that infect <i>L. mormyrus</i> from one country to another, and also from city to other cities in the same country, as in the Tunisian waters.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"1771-1778"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent.","authors":"Ragil Angga Prastiya, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, And Nining Haryuni","doi":"10.5455/OVJ.2024.v14.i8.33","DOIUrl":"10.5455/OVJ.2024.v14.i8.33","url":null,"abstract":"<p><strong>Background: </strong>The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls.</p><p><strong>Aim: </strong>This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls.</p><p><strong>Methods: </strong>Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1, 0.15, and 0.2 mg/100 ml, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (<i>p</i><0.05). Parameters observed included the assessment of fresh semen quality, kinematic analysis, post-thawing sperm viability, and abnormality.</p><p><strong>Results: </strong>The results indicated that the assessment of fresh semen quality showed macroscopic and microscopic semen quality according to SNI 4869-1:2021. Kinematic analysis revealed significant differences in DSL and STR parameters between P0 and P3 (<i>p</i><0.05). EGCG supplementation also caused significant differences in motility between P0 and P3 (<i>p</i><0.05). Viability and spermatozoa abnormality with EGCG supplementation did not show significant differences (<i>p</i>>0.05).</p><p><strong>Conclusion: </strong>The best results for motility, kinematics, and sperm morphology variables were found in P1 as it did not exhibit a decrease in motility, kinematics, and sperm morphology. Viability did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in viability with mean and standard deviation (66.84 ± 7.88). Abnormality variables also did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in abnormality with mean and standard deviation (23.80 ± 7.36).</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 8","pages":"2040-2048"},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}