Odontology最新文献

{"title":"Physical and mechanical properties of a strontium silicate-based sealer.","authors":"Shannon Wong, Xiaofei Zhu, Tun-Yi Hsu, Sami Chogle, Russell A Giordano, Yuwei Fan","doi":"10.1007/s10266-026-01317-3","DOIUrl":"10.1007/s10266-026-01317-3","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to evaluate the physical and mechanical properties of an endodontic sealer containing strontium silicates (Sr-Si sealer, C-Root SP, Beijing, China) compared to a calcium silicate-based sealer (Ca-Si sealer, Endosequence BC Sealer, Savannah, USA).</p><p><strong>Methods: </strong>Elemental composition of the sealers was confirmed by energy-dispersive X-ray spectroscopy (EDS). Afterwards, particle size was evaluated with SEM (scanning electron microscopy). Flow (n = 10 per group), setting time. (n = 5), film thickness (n = 10), and radiopacity (n = 10) were tested under ISO 6876/2012 standards. To mimic clinical applications, a pressure-flow test (n = 10) was designed and carried out to estimate the forces needed to initiate sealer flow into dentin tubules. A direct observation on sealer penetration depth inside dentin tubule was also performed by SEM with 20 extracted single-canaled teeth, obturated with either Sr-Si or Ca-Si sealer (n = 10 each) using hydraulic condensation technique, resulting in 1080 per sealer group. The data were analyzed with a t test (α = 0.05).</p><p><strong>Results: </strong>The Sr-Si sealer contained 10.6 wt% Sr and an average particle size of 1.59 ± 0.97 µm, while the Ca-Si sealer contained 27.0 wt% Ca and a particle size at 8.05 ± 5.66 µm. The Sr-Si sealer also contained 16.2% tantalum which was not found in Ca-Si sealer. In the ISO tests, the Sr-Si sealer showed longer setting time, better flowability, and radiopacity (p < 0.01). The force to initiate the flow in simulated tubules for the Sr-Si sealer was 11.00 ± 0.74 N, significantly lower than the Ca-Si sealer's 21.51 ± 2.59 N. The results were also consistent with the observation on extracted teeth that Sr-Si sealer penetrated further than Ca-Si sealer.</p><p><strong>Conclusions: </strong>The properties of the Sr-Si sealer suggest that it possesses desirable characteristics based on current in vitro criteria. While these results provide a strong foundation for its application, conclusions regarding its long-term clinical suitability depend on further clinical trials.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2485-2492"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of prognostic parameters with impact on disease-free survival rate of patients treated for oral squamous cell carcinoma: a look at a few confounding variables.","authors":"Neha Kannan, S Gheena, Pratibha Ramani, K Hema Shree","doi":"10.1007/s10266-025-01297-w","DOIUrl":"10.1007/s10266-025-01297-w","url":null,"abstract":"<p><p>Oral Squamous Cell Carcinoma (OSCC) poses a significant health concern, necessitating early prognostic studies for effective treatment planning. Traditional TNM staging has proven inadequate, prompting a need for deeper understanding of biological markers. This study evaluates the prognostic value of Hematoxylin and Eosin (H&E)-stained Depth of Invasion (DOI) compared to immunohistochemical (IHC) assessments, focusing on correlations with neck node positivity, tumor thickness, staging, perineural invasion, tumor budding, and patient survival rates. We analyzed 102 histologically confirmed OSCC cases, investigating the relationship between histological DOI, tumor budding, tumor thickness, staging, perineural invasion, recurrence, and survival. Additionally, 15 cases with borderline DOI were subjected to H&E staining and IHC to evaluate the necessity of IHC in DOI prediction. Depth of invasion (DOI) showed significant associations with tumor site (p = 0.007), tumor thickness (p = 0.000), tumor size (p = 0.000), nodal involvement (p = 0.000), perineural invasion (p = 0.001), survival (p = 0.000), and recurrence rate (p = 0.001), underscoring its role in tumor progression and prognosis. IHC demonstrated increased DOI in some cases, leading to upgraded staging and treatment changes. The association of tumor budding with various clinicopathological parameters was evaluated, showing significant correlations. Tumor budding showed significant associations with depth of invasion (p = 0.000), tumor thickness (p = 0.000), tumor size (p = 0.000), nodal involvement (p = 0.000), perineural invasion (p = 0.000), survival (p = 0.000), and recurrence rate (p = 0.003). Kaplan-Meier analysis showed lower survival rates for higher-risk patients. Histopathological analysis facilitates the classification of OSCC patients into high and low-risk groups, allowing for customized treatment strategies. Integrating histomorphologic factors with TNM staging, using H&E and IHC evaluations, can enhance OSCC prognosis accuracy, guide tailored treatments, and improve patient survival outcomes. The study was approved by the institutional review board (SRB/ SDC/ OPATH-2103/24/026).</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2349-2359"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary gland function and redox status in young adult male rats treated with the psychostimulant short-release methylphenidate.","authors":"José Vitor Furuya de Lima, Lauani Murakami Lopes, Renan José Barzotti, Larissa Victorino Sampaio, Arieli Raymundo Vazão, Rayara Nogueira de Freitas, Harim Tavares Dos Santos, Rafael Rodrigues Lima, Antonio Hernandes Chaves-Neto","doi":"10.1007/s10266-025-01248-5","DOIUrl":"10.1007/s10266-025-01248-5","url":null,"abstract":"<p><p>This study evaluated the salivary flow rate, biochemical composition of saliva, and redox status of the salivary glands in rats treated with short-release methylphenidate hydrochloride (MPH), a psychostimulant commonly used in the treatment of attention deficit hyperactivity disorder. Twenty young adult male Wistar rats (6 weeks old) were randomly assigned to two groups (n = 10): Control (0.9% saline) and MPH (3 mg/kg). After 28 consecutive days of intragastric gavage, saliva was collected following pilocarpine stimulation, for analysis of salivary flow rate (mL/min/g of salivary gland), pH, buffering capacity, and biochemical composition. Redox status markers were also assessed in the parotid and submandibular glands. The results showed that MPH treatment reduced salivary flow rate and buffering capacity without altering pH. Increases in total protein concentration, amylase activity, and concentrations of calcium and phosphate were observed, with no significant differences in sodium, potassium, or chloride content. MPH also decreased total oxidant capacity and the activities of superoxide dismutase and catalase in both salivary glands. Interestingly, oxidative damage to lipids and proteins was lower in the MPH group for the parotid glands. On the other hand, MPH reduced total antioxidant capacity and glutathione peroxidase activity in the submandibular glands, while it did not alter uric acid and reduced glutathione concentrations in either gland. In summary, MPH reduced salivary flow, altered the salivary biochemical composition, and disrupted redox homeostasis in the salivary glands. These salivary biochemical alterations may corroborate the reduction in salivary flow and contribute to oral health deterioration associated with methylphenidate use.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1903-1913"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145458833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and morphometric assessment of the middle mesial canal in mandibular first molars in a turkish population: A CBCT study.","authors":"Elif Solakoğlu, Özge Kurt","doi":"10.1007/s10266-026-01325-3","DOIUrl":"10.1007/s10266-026-01325-3","url":null,"abstract":"<p><p>This study aimed to evaluate the configurations, prevalence, and morphometric relationships of the middle mesial canal (MMC) with surrounding structures in mandibular first molars (MFMs) of a Turkish population using cone-beam computed tomography (CBCT). CBCT images of 2,999 retrospectively selected MFMs were examined. The presence and configuration of the MMC and its relationship with the mesiobuccal (MB) and mesiolingual (ML) canals were assessed. In addition, the distances between the MB-ML, MB-MMC, and ML-MMC canal orifices and the vertical distances from the MB, ML, and MMC orifices to the pulpal floor (PF) were measured. Buccolingual (BL) and mesiodistal (MD) dimensions and their ratios were also analyzed in relation to the presence of the MMC. An MMC was detected in 24.5% of the examined teeth. Among these, 77.8% exhibited a configuration merging with the MB canal. The presence of an MMC was significantly associated with a smaller BL width and a larger MD width (p < 0.001). The MMC orifice was positioned deeper than the MB and ML orifices. In the Turkish population, MMCs were observed in 24.5% of MFMs, most commonly showing a merging configuration with the MB canal. The presence of an MMC can be predicted by specific morphometric parameters, and access can be facilitated by troughing between the MB and ML canals. These findings highlight the importance of anatomical awareness of the MMC to enhance clinical success.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2550-2562"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of vertical deficiency in infraoccluded primary molars: an investigation of dental, gingival, and alveolar parameters.","authors":"Teresa Temming, Lisa Bondarjanska, Robert Strenger, Anahita Jablonski-Momeni, Heike Korbmacher-Steiner","doi":"10.1007/s10266-025-01306-y","DOIUrl":"10.1007/s10266-025-01306-y","url":null,"abstract":"<p><p>The study aims to evaluate the vertical deficiency associated with infraoccluded primary molars by quantitatively analyzing dental, gingival, and alveolar parameters, and to determine their potential diagnostic and therapeutic relevance in clinical practice. A total of 290 infraoccluded primary molars from 129 patients under 30 years of age were retrospectively analyzed (data collection between 2000 and 2023). Inclusion required at least one infraoccluded primary molar (> 0.5 mm), along with diagnostic dental casts and panoramic radiographs taken within six months. Parameters evaluated included the degree of infraocclusion, gingival margin levels, and alveolar ridge morphology. The presence or absence of the successor tooth was considered. The mean vertical displacement of infraoccluded molars was 1.6 mm (SD 0.87). Atypical gingival margins were observed in 35% (n = 122) of infraoccluded molars, compared to 4% (n = 2) in controls, with higher prevalence in the mandible and second primary molars. The frequency of atypical gingival margins and alveolar ridge morphologies increased with the severity of infraocclusion (P < 0.05, respectively). An infraocclusion of ≥ 2.0 mm predicted atypical alveolar morphology with 70.6% sensitivity and 87.6% specificity. A significant association was also observed between atypical alveolar ridges and atypical gingival margins (P < 0.05). Multiple regression analysis (adjusted R² = 0.42) revealed that infraocclusion severity was significantly associated with age, sex, tooth type, gingival margin, and alveolar ridge morphology, whereas agenesis of the permanent successor showed no significant association. Infraocclusion of primary molars influences not only occlusal relationships but also gingival and alveolar morphology. Atypical gingival and alveolar ridge patterns may offer valuable supplementary diagnostic criteria for the management of dental ankylosis. Further prospective studies are needed to investigate their potential for use in clinical decision-making.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2395-2404"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do universal adhesives applied with different modes affect remineralized enamel surfaces? A SEM study.","authors":"Beyza Arslandaş Dinçtürk, Cemile Kedici Alp, Hanife Altinişik, Ceyda Sari","doi":"10.1007/s10266-025-01290-3","DOIUrl":"10.1007/s10266-025-01290-3","url":null,"abstract":"<p><p>The aim of this study is to evaluate the effect of different application modes of different universal adhesives on enamel shear bond strength after remineralization on white spot lesions and to evaluate the changes in the remineralized surface with SEM/EDX analysis. 144 bovine enamels were obtained for shear bond strength testing, and 120 enamel were obtained to evaluate the effect of adhesives on Ca/P ratios. Demineralization and remineralization values were measured using DIAGNOdent Pen and randomly distributed into 12 groups (n = 12). Universal adhesives (G-Premio Bond, Clearfil S³ Universal Bond) were applied to intact, demineralized, remineralized enamels according to total-etch and self-etch modes, then resin composite (R = 1 mm, h = 2 mm) was placed and polymerized. Changes in Ca/P ratio with the removal of adhesives from enamels were evaluated by EDX. Data were analyzed with three-way ANOVA test and post hoc Tukey HSD test. According to the data, there are statistically significant differences in universal adhesives, application modes, bond strength, and Ca/P ratios between different enamel surfaces. For both adhesive systems, highest bond strength was obtained on sound enamel samples with the total-etch adhesive mode. In remineralized enamel, bond strength in total-etch mode decreased when G-Premio Bond was used compared to self-etch system and increased when Clearfil S³ Universal Bond was used. Use of Remin Pro, a remineralization agent containing xylitol, hydroxyapatite and fluoride, increased bonding on enamel surface compared to demineralized enamel, and this effect is close to bonding to intact enamel surface. Universal adhesives change the Ca/P ratios on remineralized surface. To increase the bond strength on this surface layer, it can be recommended to remineralize the surface before adhesive treatments because of the weakened bond strength. In both universal adhesives, bonding to remineralized tooth enamel produced bond strength values similar to those on intact tooth enamel. Bond strength increases when the Remin Pro remineralization agent is applied to tooth enamel surfaces. Applying universal adhesive with mild-acidity in the self-etch mode, rather than a total-etch mode to remineralized enamel increased bond strength. Applying a light-acidity universal adhesive in the total-etch mode increased the bond strength. Using orthophosphoric acid in remineralized enamel should be exercised carefully.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2244-2255"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rg1 activates the AMPK/mTOR-autophagy axis and inhibits the NOD-like receptor 3 inflammasome to alleviate pyroptosis in periodontal ligament fibroblasts.","authors":"Di Wang, Ying Zhang, Lei Yang","doi":"10.1007/s10266-025-01304-0","DOIUrl":"10.1007/s10266-025-01304-0","url":null,"abstract":"<p><p>This study explored the mechanism of ginsenoside Rg1 (Rg1) alleviating lipopolysaccharide (LPS)-induced pyroptosis in human periodontal ligament fibroblasts (HPDLFs). HPDLFs were treated with LPS to induce pyroptosis, followed by the Rg1 intervention. Cell viability and lactate dehydrogenase (LDH) release were assessed by CCK-8 and kits. Levels of pyroptosis-related cytokines, NOD-like receptor 3 (NLRP3) mRNA expression, and protein levels of NLRP3 inflammasome-related markers, pyroptosis-related markers, autophagy-related markers, and the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR) pathway were determined by ELISA, RT-qPCR, and western blot. Autophagosome changes were also observed under transmission electron microscopy. HPDLFs were transfected with NLRP3-overexpression plasmids, or co-treated with LPS + Rg1 and the autophagy inhibitor (3-MA), AMPK inhibitor (BML-275), or mTOR activator (MHY1485). LPS increased pyroptosis (decreased cell viability, increased LDH release, increased GSDMD-N-terminal domain protein and IL-1β and IL-18 levels), activated the NLRP3 inflammasome (increased NLRP3, apoptosis-associated speck-like protein containing a CARD, cleaved Caspase-1 protein levels), induced autophagy (increased Beclin1 protein, LC3-II/LC3-I levels, and autophagosome number), impaired autophagic flux, and inhibited p62 degradation. Partial AMPK activation and mTOR inhibition were observed. LPS + Rg1 further activated AMPK/mTOR signaling, mitigated impaired autophagic flux, inhibited the NLRP3 inflammasome, and alleviated HPDLF pyroptosis. Rg1 promoted autophagy by regulating the AMPK/mTOR pathway to inhibit NLRP3 inflammasome activation, thereby inhibiting HPDLF pyroptosis. AMPK inhibition or mTOR activation partially averted Rg1's regulatory effects. Rg1 activates AMPK/mTOR-autophagy axis and inhibits NLRP3 inflammasome, thereby alleviating LPS-induced HPDLF pyroptosis.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2380-2394"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementary instrumentation did not enhance the removal of residual gutta-percha: a micro-computed tomography study.","authors":"Selin Nur Ayaz, Meltem Kucuk, Deniz Yanık Nalbantoğlu, Ali Keles, Amine Yigit, Fugen Dagli Comert Tasman, Bekir Karabucak","doi":"10.1007/s10266-025-01260-9","DOIUrl":"10.1007/s10266-025-01260-9","url":null,"abstract":"<p><p>We evaluated the effect of supplementary instrumentation on the removal of gutta-percha and compared the efficacy of three file systems with different metallurgical properties via micro-computed tomography (micro-CT). Forty-two moderately curved mandibular molars were selected and divided into 3 groups (n = 14): group-PTG: ProTaper Gold, group-PTN: ProTaper Next, and group-VB: Vortex Blue. Only mesial roots with Vertucci type-IV configuration were included. Canals were instrumented and obturated with bioceramic sealer. Retreatment was performed using a retreatment file system, and supplementary instrumentation was completed according to experimental groups: ProTaper Next X4 (40/06), ProTaper Gold 40/06, and Vortex Blue 40/06. Initial, second (after using the retreatment file system), and final (after supplementary instrumentation) micro-CTs were obtained (resolution 85 kV, 118 µA, and 13.6 µA pixel size), and residual gutta-percha at apical, middle, and coronal thirds was calculated. One-way ANOVA and Student's t test were performed for statistical analysis. While the first and second micro-CT values demonstrated a statistical difference (p < 0.05), there was no difference between the second and final micro-CT values. No differences were detected between group-PTN (96.89-98.32%), group-PTG (95.36-98.51%), and group-VB (%96.96-%99.38). Residual gutta-percha volumes were similar in mesiobuccal and mesiolingual canals. Supplementary instrumentation did not decrease the amount of residual gutta-percha. Increasing the initial apical size by three sizes removed 98.18% to 99.38% of gutta-percha. ProTaper Next, ProTaper Gold, and Vortex Blue showed similar effects. In the apical third of mesiobuccal, ProTaper Gold and Vortex Blue eliminated gutta-percha more effectively than ProTaper Next.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2030-2039"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145588563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIF2AK2 activates autophagy via JAK2/STAT3 pathway to promote oral squamous cell carcinoma malignancy.","authors":"Xiaonan Zhang, Xiangling Liao, Zhen Du","doi":"10.1007/s10266-025-01298-9","DOIUrl":"10.1007/s10266-025-01298-9","url":null,"abstract":"<p><p>The progression of oral squamous cell carcinoma (OSCC), a frequent cancer of the mouth, has been associated with EIF2AK2 dysregulation, but its precise mechanisms are yet to be clarified. In this study, we investigated the expression of EIF2AK2 in OSCC tissues and cell lines using publicly available databases and Western blotting. EIF2AK2 was silenced using small interfering RNA (siRNA), and its functional impact on OSCC malignant characteristics was determined using several in vitro experiments. Additionally, we determined EIF2AK2's impact on autophagy levels and the activity of mechanistic pathways. Our results demonstrate that EIF2AK2 is markedly overexpressed in OSCC tissues and cell lines, and its knockdown significantly affects OSCC malignant characteristics, such as proliferation, migration, and invasion. Furthermore, EIF2AK2 knockdown increased E-cadherin levels while reducing those of N-cadherin and Vimentin, supporting EMT suppression. It also led to reduced autophagy based on decreased LC3-II and Beclin-1 and increased p62 expressions. Moreover, inhibition of EIF2AK2 led to the downregulation of the JAK2/STAT3 axis. Collectively, these findings support EIF2AK2 as an oncogene in OSCC progression by activating autophagy through the JAK2/STAT3 pathway.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"2321-2329"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional aloe vera-based biomaterial for vital pulp therapy: an in vivo evaluation.","authors":"Sharon S Namazi, Pedro Henrique Chaves de Oliveira, Neville J McDonald, Bruno Cavalcanti, Hajime Sasaki, Marco C Bottino, Renan Dal-Fabbro","doi":"10.1007/s10266-025-01258-3","DOIUrl":"10.1007/s10266-025-01258-3","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of Gelatin methacryloyl/Aloe vera (GelMA/AV) scaffolds, both alone and in combination with mineral trioxide aggregate (MTA), on pulp inflammatory response and mineralized tissue formation in a rat model of pulp exposure. GelMA/AV nanofibers (70:30) were fabricated via electrospinning and photocrosslinked. Twenty-four rats were randomly assigned to three groups (n = 8/ group): GelMA/AV, GelMA/AV + MTA, and MTA (control). Following pulp exposure of the first upper molars (n = 2/animal), the materials were placed over the exposed pulp as the pulp capping agent, and MTA was applied or not, depending on the group. The cavities were then sealed with glass ionomer. Animals were euthanized at 7 or 28 days post-procedure for histological, immunohistochemical, and micro-computed tomography (micro-CT) analyses. Data were analyzed using one-way or two-way ANOVA at a 5% significance level. Micro-CT analysis at 28 days revealed new hard tissue formation in the pulp chamber across all groups. GelMA/AV + MTA demonstrated comparable mineralized volume and tissue mineral density to MTA. Histologically, the GelMA/AV + MTA group exhibited a significantly lower cell count than the MTA and GelMA/AV groups at 28 days. However, for macrophage polarization, the GelMA/AV + MTA exhibited a similar expression of CD163-positive (M2) and iNOS-positive (M1) cells compared to the MTA. Lastly, GelMA/AV + MTA also showed expression of nestin and DMP1 at 28 days, indicating active mineralization. In conclusion, a thin GelMA/AV sublayer beneath MTA preserves MTA-driven mineralization but does not reduce pulp inflammation or enhance mineralization beyond MTA in this model. Optimization of AV dosing/formulation and longer follow-up are necessary before clinical translation.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1992-2001"},"PeriodicalIF":2.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13011884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145541527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}