Odontology最新文献

{"title":"Five-year outcomes of patient quality of life, satisfaction, and preconceptions following root canal treatment, re-treatment, or single dental implant placement in the anterior zone.","authors":"Carlota de España, Juan Gonzalo Olivieri, Jordi Ortega, Sergio Morelló, Marc Encinas, Fernando Durán-Sindreu","doi":"10.1007/s10266-025-01199-x","DOIUrl":"https://doi.org/10.1007/s10266-025-01199-x","url":null,"abstract":"<p><p>The primary aim of this study was to compare the overall satisfaction and quality of life (QoL) up to 5 years after treatment in patients who underwent root canal treatment (RCT), re-treatment (re-RCT), or single tooth implant (STI) placement in the anterior zone. The secondary aim was to analyze factors influencing decision-making and patients' fear levels. This cross-sectional study entailed face-to-face personal interviews performed 5 years after treatment. The procedures were performed in private practice by two experienced dentists, specialists in Endodontics and Oral Surgery and Implantology, respectively. The Chi-squared test was used to evaluate the differences in treatment decision-making. Factors influencing treatment decisions were compared using the Kruskal-Wallis test, while ANOVA was employed to assess Oral Health Impact Profile (OHIP)-14 scores and overall satisfaction. Significance was set at p < 0.05. Most patients reported that decisions were made jointly with their dentist, with no differences among groups (p > 0.05). Esthetics were the most influential factor in decision-making (p < 0.05). No differences were found in overall satisfaction among the groups (p > 0.05). STI treatment induced the highest perceived fear and pain scores (p < 0.05). Significant differences were found in some domains of the OHIP-14 in the STI group compared with the RCT and re-RCT groups (p < 0.05). No differences were found between the RCT and re-RCT groups (p > 0.05). Endodontic treatments (RCT and re-RCT) yielded high patient QoL and satisfaction levels comparable to STI, while resulting in lower social disability, psychological discomfort, and physical disability. Esthetics were the most influential determinant across all procedures. In addition, STI resulted in significantly higher perceived fear and pain levels compared with endodontic procedures. The present study's findings underscore the importance of personalized, patient-centered approaches in dental practice to enhance long-term patient satisfaction and outcomes.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of theaflavin-based dental varnishes against erosive demineralizatıon and matrix metalloproteinase expression.","authors":"G Ozan, H Sar Sancakli, S Batu, A Gurbuz Yurtsever, M S Erdal, A Sengelen, E Onay Ucar, S Ozel Yildiz, B Bicak, S Kecel Gunduz","doi":"10.1007/s10266-025-01177-3","DOIUrl":"https://doi.org/10.1007/s10266-025-01177-3","url":null,"abstract":"<p><p>The objective of the present study was to evaluate the efficacy of experimental varnishes containing theaflavin (TF-2a/2b) against erosive demineralization and matrix metalloproteinase enzymes (MMPs) and investigate the bonding properties of TFs to MMPs. Enamel and dentin samples, measuring 3 × 5 × 2 mm, were extracted and immersed in citric acid solution for 60 s to induce artificial erosive lesions. The samples were then randomly allocated into six experimental groups and one control (fluoride) group (n = 6). Experimental groups were treated with different varnish formulations of resin-, polymer-, or resin + polymer-based varnishes containing either TF-2a or TF-2b. Following immersion in artificial saliva, all samples were subjected to a 5-min pH cycle for 5 days. Mineral density (MD) values were assessed using MicroCT, and expression levels of MMPs were evaluated by Western Blot. In silico analyses of both TFs were conducted against MMP-2 and MMP-9. Mineral density values of all groups increased significantly following the pH cycle [TF-2b resin + polymer-based (p = 0.023); all other groups (p = 0.026)]. Varnishes based on TF-2b demonstrated numerically superior results in MD scores, but resin-polymer-based TF-2a showed better MMP expressions than other groups. The binding energies of both tested TFs with MMP-2 and MMP-9 were determined to be similar. Profile analysis of both TFs are also indicated to be effective against the activity of MMPs. Therefore, resin-based TF-2a and TF-2b experimental varnishes may serve as viable natural alternatives to fluorides in managing dental erosion.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking systemic inflammation to periodontal disease: insights from blood-derived biomarkers in NHANES.","authors":"Peng Huang, Rui Zhang, Yiwen Sheng, Binbin Nan, Xiaojun Li, Zijian Cheng","doi":"10.1007/s10266-025-01213-2","DOIUrl":"https://doi.org/10.1007/s10266-025-01213-2","url":null,"abstract":"<p><p>Periodontitis is a prevalent inflammatory disease linked to systemic immune activation and associated with various chronic conditions. Blood-derived inflammatory markers, such as the albumin-to-globulin ratio (AGR), may offer insights into systemic inflammation related to periodontitis, yet their role remains underexplored. This study aimed to investigate the association between AGR and other inflammatory markers with periodontitis in a representative U.S. adult population. A cross-sectional analysis was conducted using data from the National Health and Nutrition Examination Survey (NHANES) 2009 to 2014, including 10,135 adults aged ≥ 30 years with complete periodontal and laboratory data. Periodontitis was defined using CDC/AAP criteria. AGR, systemic immune-inflammatory index, neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio and platelet-to-lymphocyte ratio were calculated from routine blood parameters. Associations between periodontitis and those inflammatory markers were assessed using multivariable logistic regression adjusted for sociodemographic, lifestyle, metabolic factors, systemic conditions, and liver function markers. Non-linear relationships were explored with restricted cubic splines. AGR was inversely associated with periodontitis in all models (Model 3: OR = 0.62, 95% CI: 0.49-0.78; P < 0.001). A significant L-shaped non-linear relationship was observed, with a threshold at AGR = 1.778. Below this value, each unit increase in AGR was associated with a 54% lower odds of periodontitis (OR = 0.46, 95% CI: 0.34-0.63). No significant non-linear associations were found for albumin, globulin, SII, PLR, or LMR. A significant gender interaction was detected (P < 0.001). Lower AGR was independently associated with greater odds of periodontitis, highlighting the potential role of systemic inflammatory biomarker in the pathophysiology of periodontitis. Longitudinal studies are needed to confirm causality.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological analysis of 30 specimens of glandular odontogenic cyst.","authors":"Junya Ono, Yasuo Okada, Ichiro Ogura, Shuji Toya","doi":"10.1007/s10266-025-01159-5","DOIUrl":"https://doi.org/10.1007/s10266-025-01159-5","url":null,"abstract":"<p><p>Glandular odontogenic cysts (GOC) are relatively rare, and require differentiation from radicular cysts, dentigerous cysts, and central mucoepidermoid carcinoma of jawbone. We conducted clinical, histopathological, and immunohistochemical analysis to elucidate the relationship between the clinical behavior and histopathological features, as well as the origin of tissues and factors related to the development of GOC. Thirty specimens from 29 patients (19 males, 11 females; mean age 51.8, range 18-85 years) diagnosed histopathologically with GOC were studied. By location site, mandibular molar region was most common (17 cysts), followed by maxillary anterior region (5), maxillary molar region (4), and mandibular anterior region (4). All cases were clinically asymptomatic and were detected incidentally on X-ray examination. In the X-ray findings, all cases showed unilocular, well-defined radiolucencies, with a long diameter ranging from 9 to 36 mm. Histopathologically, eosinophilic cuboidal cells and microcysts were found in all cases, apocrine snouting was observed in 27 (90%), clear cells in 28 (93.3%), variable thickness of epithelial lining in 29 (96.7%), and mucous cells (both PAS and d-PAS positive) in 29 (96.7%). These six histopathological features were useful for histopathological diagnosis. There was one case of multiple metachronous bilateral mandibular lesions and one case of a locally aggressive type. Immunohistochemical staining showed CK19 positivity and BRAF positivity in all cases, and CK18 positivity in 24 (80%). Ki-67 was positive in the lining epithelium of a locally aggressive case, but positive cells were rare in other cases. CK19 positivity in all cases indicate that GOCs were derived from odontogenic epithelium; and BRAF positivity suggests involvement of this gene mutation in GOC development. Ki-67 positivity may indicate locally aggressive type, which warrants further study.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orofacial pain and dementia: a brief narrative review of associations, putative mechanisms, and relevant clinical considerations.","authors":"Alberto Herrero Babiloni, Sylvia Villeneuve, Pierre Rainville, Gilles J Lavigne, Cibele Dal Fabbro","doi":"10.1007/s10266-025-01216-z","DOIUrl":"https://doi.org/10.1007/s10266-025-01216-z","url":null,"abstract":"<p><p>This review examines the possible association between orofacial pain and dementia by synthesizing current findings from clinical, observational, and mechanistic studies. Although chronic pain has been increasingly recognized as a factor linked to cognitive decline, the specific role of orofacial pain, including conditions such as temporomandibular disorders, trigeminal neuralgia, burning mouth syndrome, and primary headaches, remains underexplored. Given the distinct neuroanatomy of the trigeminal system and its close connection to emotional and cognitive processing, orofacial pain may involve unique mechanisms relevant to neurodegeneration. Studies suggesting possible links between orofacial pain conditions and cognitive impairment were identified, highlighting mechanisms such as neuroinflammation, locus coeruleus dysfunction, and central sensitization. Modifiable cofactors, including poor oral health and sleep disturbances, which may influence both pain and dementia risk and are particularly relevant in dental and orofacial clinical practice, were also examined. Pain assessment and management strategies for individuals with dementia were reviewed as well, with emphasis on the need for validated tools and interdisciplinary care models that incorporate dental and orofacial pain specialists. Given the heterogeneous and limited nature of existing studies, this review does not make causal claims but instead conceptually maps the current landscape, identifies critical gaps in the literature, and outlines implications for research and practice. Dentists and orofacial pain providers may be uniquely positioned to recognize pain-related risk factors in cognitively vulnerable patients and implement preventive or therapeutic strategies. Future longitudinal studies and mechanistic investigations are needed to clarify the direction and clinical significance of the orofacial pain-dementia relationship.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable GSK3 inhibitor (Tideglusib) hydrogel versus enhanced β-tricalcium phosphate in tibial bone healing (in vitro and in vivo study).","authors":"Mahitabe Elgamily, Mona Denewar, Ahmed A Emam, Basma Hamed Othman, Nessma Sultan","doi":"10.1007/s10266-025-01203-4","DOIUrl":"https://doi.org/10.1007/s10266-025-01203-4","url":null,"abstract":"<p><p>This study was carried out to assess the potential effect of a glycogen synthase kinase-3 inhibitor tideglusib hydrogel formulation on bone regeneration and to compare it with Ethoss a beta tricalcium phosphate alloplastic bone substitute mixed with calcium sulphate in vitro and in vivo. Bone marrow mesenchymal stem cells (BMMSCs) were isolated from New Zealand white rabbit tibias and characterized by flowcytometric analysis. Sodium alginate hydrogel loaded with tideglusib was formulated and underwent characterization by scanning electron microscopy, the fourier transform infrared and zeta potential. Extraction media from both tideglusib hydrogel and Ethoss were prepared then MTT, alizarin red (ARS) and scratch wound assays were performed. Two bone defects were trephined in tibias of eighteen adult healthy rabbits, which were classified into three groups: group 1, bone defects were left untreated; group 2, each defect was filled with tideglusib hydrogel; group 3, each defect was filled with Ethoss. Animals were sacrificed after three and six weeks. Histological and immunohistochemical analyses using alkaline phosphatase (ALP) and osteopontin (OPN) were performed to assess bone regeneration. The tideglusib hydrogel group expressed higher mineral deposition than Ethoss group using ARS but without significant difference, stronger stimulatory effect on the migration of BMMSCs was also evident with significant narrowing of the wound area. New bone tissue was progressively seen bridging and filling the defects with significantly higher ALP and OPN expression in hydrogel group. Tideglusib hydrogel formulation exhibited superior bone regenerative potential in comparison to beta tricalcium phosphate alloplastic bone substitute.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-surgical periodontal treatment effects on IL-17 and IL-35 levels in smokers and non-smokers with periodontitis.","authors":"Taskaldiran Ezgi Sila, Tuter Gulay, Atak Yucel Aysegul, Yaman Melek","doi":"10.1007/s10266-025-01207-0","DOIUrl":"https://doi.org/10.1007/s10266-025-01207-0","url":null,"abstract":"<p><p>Periodontitis, a chronic inflammatory disease influenced by host-microbiota interactions, poses a significant global health burden. Smoking exacerbates periodontitis progression and impacts treatment outcomes. Interleukin (IL)-17 and IL-35 are key mediators of inflammation, yet their roles in smoking-related periodontitis and responses to non-surgical periodontal treatment (NSPT) remain underexplored. This clinical trial aimed to evaluate the effects of NSPT on salivary and gingival crevicular fluid (GCF) levels of IL-17 and IL-35 in smokers and non-smokers with periodontitis. A total of 55 subjects were divided into three groups: smokers with periodontitis (SP), non-smokers with periodontitis (NSP), and healthy controls (C). All subjects underwent comprehensive periodontal assessments and cytokine level analyses at baseline and four weeks post-NSPT. Saliva and GCF samples were analysed for IL-17 and IL-35 using enzyme-linked immunosorbent assay (ELISA). Statistical analyses evaluated group differences and correlations. After NSPT, both SP and NSP groups exhibited significant improvements in clinical parameters (p < 0.05). Salivary IL-17 and IL-35 levels did not show significant differences between the SP, NSP, and C groups after NSPT (p > 0.05). However, in GCF, IL-17 and IL-35 concentrations significantly increased in both SP and NSP groups following NSPT (p < 0.05). The total amount of GCF IL-17 decreased significantly only in the NSP group after NSPT (p < 0.05), while the total amount of GCF IL-35 increased significantly in both SP and NSP groups (p < 0.05). Strong correlations between IL-17 and IL-35 levels in both saliva and GCF were observed. NSPT modulates inflammatory and anti-inflammatory cytokine profiles, promoting improved clinical outcomes. The impact of smoking on cytokine responses highlights the need for further research to elucidate the mechanisms underlying these cytokine modulations and their role in periodontal therapy. Trial registration ClinicalTrials.gov ( https://clinicaltrials.gov -01.01.2018-NCT05281848).</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and antimicrobial profiling of glycolic and etidronic acid as endodontic irrigants.","authors":"Jardel Dorigon-Santos, Geovanna de Oliveira Costa, Heloisa Cardoso Martins, Julia Roberta Régis, Maurício Malheiros Badaró, Niels Salles Willo Wilhelmsen, Juliana Silva Ribeiro, Maira do Prado, Louis Pergaud Sandjo, Thais Mageste Duque","doi":"10.1007/s10266-025-01183-5","DOIUrl":"https://doi.org/10.1007/s10266-025-01183-5","url":null,"abstract":"<p><p>This study aimed to analyze the chemical interactions between commonly used endodontic irrigants and evaluate the antimicrobial efficacy of etidronic acid (HEDP), glycolic acid (GA), and ethylenediaminetetraacetic acid (EDTA) as final irrigants. The solutions tested included 9% HEDP, 17% GA, 17% EDTA, 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) gel, and distilled water (DW). Solutions were mixed in a 1:1 ratio and analyzed using electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF). The pH values were measured with a calibrated pHmeter. For the microbiological assay, ninety mandibular premolars were inoculated with Enterococcus faecalis and incubated for 5 days. Teeth were randomly assigned to five groups (n = 18), according to the final irrigation protocol: 17% EDTA, 17% GA, 9% HEDP in NaOCl, 9% HEDP in saline (SS), and SS alone. Microbial sampling was performed before and after chemomechanical preparation and final irrigation. Colony-forming units per milliliter (CFU/mL) were quantified, log-transformed, and statistically analyzed using Friedman and Dunn's post hoc tests. Chemical analysis revealed precipitate formation in the mixtures of NaOCl with CHX gel, NaOCl with EDTA, CHX gel with HEDP, and CHX with EDTA. No p-chloroaniline (PCA) or other toxic byproducts were detected. GA, HEDP and EDTA irrigants reduced bacterial load. These results highlight the potential of the solution as an effective final irrigant and underscore the importance of understanding chemical interactions among irrigants to prevent adverse effects during endodontic therapy.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of low-dose gamma radiation and/or clove oil loaded on silica nanoparticles on regeneration of human gingival fibroblast cell line: an in vitro study.","authors":"Asmaa M Abdeen, Mustafa Gharib, Ibrahim Y Abdelrahman","doi":"10.1007/s10266-025-01198-y","DOIUrl":"https://doi.org/10.1007/s10266-025-01198-y","url":null,"abstract":"<p><p>In light of the growing use of natural products in medicinal therapy, this study evaluated the proliferative effects of clove oil (CO) in two forms-native and loaded onto silica nanoparticles (CO@SNPs)-on Human Gingival Fibroblasts (HGFs), both singly and in combination with low-dose gamma irradiation (0.25 Gy). Silica nanoparticles were chemically synthesized and loaded with clove oil, with thorough characterization conducted using dynamic light scattering (DLS) for particle size, zeta-potential measurements, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The EC50 values of CO and CO@SNPs were determined to be 1.88 µmol/mL and 0.172 µmol/mL, respectively, demonstrating a superior proliferative efficacy of the CO@SNPs nanoformulation. The biological effects were assessed using the MTT assay for cell viability, Trypan blue exclusion for cell count, qPCR for gene expression of c-Myc, MAPK, and NF-κB, and antioxidant capacity assays (MDA and catalase activity). Moreover, the in vitro wound-healing scratch assay showed enhanced fibroblast migration and proliferation, with relative wound density reaching 48% and 62% for CO@SNPs alone and combined with low-dose gamma irradiation, respectively. Further analysis through immunofluorescence staining of TGF-β and hematoxylin staining confirmed cell proliferation. Our results showed that loading CO on silica nanoparticles (CO@SNPs) were significantly more efficient than native clove oil in promoting cell proliferation and wound healing, with the combined treatment of CO@SNPs and low-dose gamma irradiation producing the most pronounced regenerative effect. These findings suggest that CO@SNPs, especially when combined with low-dose gamma irradiation, hold significant promise for enhancing oral tissue regeneration.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential protective effect of naringenin against cadmium-induced toxicity in gingival cells.","authors":"Mahitabe Elgamily, Bassant Mowafey, Nesreen Nabil","doi":"10.1007/s10266-025-01187-1","DOIUrl":"https://doi.org/10.1007/s10266-025-01187-1","url":null,"abstract":"<p><p>The objective of the study was to evaluate the potential protective effect of naringenin against cadmium (Cd)-induced toxicity in human gingival cells. The safe dose of naringenin and the half-maximal inhibitory concentration (IC50) of Cd were defined using the methyl thiazolyl tetrazolium (MTT) assay. Then four groups were assigned: group I: control (cells + media), group II: cells + Cd, group III: cells + naringenin, group IV: cells  + Cd + naringenin. The cell viability was assessed by MTT assay. Lipid peroxidation (MDA), super oxide dismutase (SOD), and apoptosis by (Annexin V/PI) assay were measured. The ultrastructural changes in the gingival cells were observed by transmission electron microscopy. Cadmium decreased the viability of human gingival cells in a dose-dependent manner with an IC50 of 11.16 μM. However, naringenin at a concentration of 50 μM increased the cell viability significantly, but with higher concentrations the viability began to decrease. The exposure of the gingival cells to Cd significantly increased MDA levels and decreased the SOD. It also enhanced apoptosis in the gingival cells with subsequent ultrastructural changes such as cell blebs and nuclear fragmentation. The addition of naringenin improved the cell viability, decreased MDA, increased SOD, decreased cell apoptosis, and improved ultrastructural changes, suggesting a potential protective effect against cadmium toxicity in gingival cells. Naringenin has a potential protective effect against cadmium-induced toxicity in gingival cells.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}