Odontology最新文献

{"title":"Computational fluid dynamics analysis of conventional irrigation and the combination with adjuvant suction cannulas in human molars with isthmus communication.","authors":"José M R Zaldívar, Gaizka Loroño, Jesus R Jimenez-Octavio, Saul Dorado, Ana Arias","doi":"10.1007/s10266-024-01028-7","DOIUrl":"https://doi.org/10.1007/s10266-024-01028-7","url":null,"abstract":"<p><p>This study evaluated CFD key irrigation parameters (flow pattern, irrigant velocity, wall shear stress and apical pressure) of conventional irrigation with positive pressure side-vented (SV) needle and the combination of different suction cannulas in the mesial root of human mandibular molars with 2 independent root canals and isthmus communication. A micro-CT scan of a molar presenting 2 root canals and an isthmus communication in the mesial root was obtained for computational analysis after root canal preparation and geometric reconstruction. Computational models of a 30G SV needle and three different suction cannulas (EndoVac Macro cannula (MaC), Surgitip (SURG) and iNP needle (iNP)) were designed. Four different simulations were carried out: simulation SV (SV needle located at 2.5 mm from working length (WL) in mesiolingual root canal (ML)), simulations SV-iNP, SV-SURG and SV-MaC (SV needle in ML plus the respective suction cannula placed at 7.5 mm from WL in mesiobuccal root canal). Key magnitudes of irrigation were compared with CFD. The irrigant solution did not enter the isthmus in SV simulation. Simulations SV-iNP, SV-SURG and SV-MaC exhibited a better performance in shear stress and apical pressure. Fluid flow did not reach the apical millimeters of the main root canal, but substantial flow and shear stress was observed in the isthmus in SV-SURG and SV-MaC simulations. The use of suction cannulas aids conventional irrigation in roots with 2 independent root canals and isthmus communication by improving irrigant flow, increasing shear stress in the root canal walls and isthmus, and slightly decreasing apical pressure.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-assisted Icon and clinpro for restoring white spot lesions: an in vitro comparative study.","authors":"Yomna Said Mohamed, Mohamed Shamel, Sara El Banna","doi":"10.1007/s10266-024-01026-9","DOIUrl":"https://doi.org/10.1007/s10266-024-01026-9","url":null,"abstract":"<p><p>Managing white spot lesions (WSLs) remains a challenging issue that has yet to be fully resolved. WSLs are areas of demineralized enamel that most commonly occur following orthodontic treatments. They can potentially lead to enamel caries and are also esthetically undesirable. The current study investigated and analyzed the effects of Icon resin infiltration (Icon) and Clinpro XT varnish (Clinpro), both alone and in combination with a diode laser, on the restoration of WSLs. Color change, microhardness, and scanning electron microscopy were used to evaluate the WSLs after the different treatment applications. Results showed that the combination of Icon and Clinpro, along with a diode laser, enhanced color stability and restoration of enamel hardness in white spot lesions. Utilizing a diode laser significantly improved the efficacy of both Icon and Clinpro therapies. SEM examination verified that laser-assisted treatments resulted in almost total blockage of enamel rods, indicating enhanced effectiveness. Conclusions: Integrating diode laser treatment with Icon and Clinpro XT Varnish has significantly improved the esthetic outcomes and mechanical properties of treated enamel.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of different kinematics on shaping ability and accumulated hard tissue debris: an ex vivo study.","authors":"Virginia Rosy Romeo, Giusy Rita Maria La Rosa, Luigi Generali, Eugenio Pedullà, Daniele Angerame","doi":"10.1007/s10266-024-01025-w","DOIUrl":"https://doi.org/10.1007/s10266-024-01025-w","url":null,"abstract":"<p><p>To determine the shaping ability, the amount of accumulated hard tissue debris (AHTD) and untouched area is generated by different kinematics through micro-CT. Thirty-two mesial roots of mandibular molars with Vertucci type IV were pre-scanned through micro-CT evaluation and randomized to one of the four experimental groups according to the kinematics tested: Continuous Rotation (CR), Reciprocation (REC), Optimum Torque Reverse (OTR) and Jeni motion. The root canal preparation was performed with HyFlex EDM (Coltène/Whaledent AG) up to OneFile. After each file used, irrigation was performed with 2 mL 5.25% NaOCl for 1 min. The final rinse was performed with 1 ml of 5.25% NaOCl followed by a 1 ml of 17% EDTA and, 1 ml of distilled water. Then, the samples were scanned and the images, before and after preparation, were superimposed and analyzed to determine the AHTD, untouched areas, canal transportation, centering ability, and shaping time. Data were statistically analyzed using Kruskal-Wallis test and Dunn post hoc with p < 0.05. No significant differences were detected among the tested kinematics in terms of untouched areas, AHTD, canal transportation and shaping time (p > 0.05). Significantly more untouched areas were detected in the coronal third compared to the middle (p = 0.0301) and apical ones (p = 0.0399) under continuous rotation. Moreover, a significantly higher centering ratio in the middle third compared to the apical was observed for Jeni motion (p = 0.0207). The tested kinematics showed similar untouched areas, AHTD, shaping ability and shaping time. All motions determined an appropriate mechanical preparation; yet, none of them was able to prepare all canal walls and keep the root canals completely free from hard tissue debris.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hydrogen peroxide diffusion in the pulp chamber, bleaching efficacy, and physicochemical properties of an experimental in-office bleaching gel containing a hydroxyapatite-capsaicin composite.","authors":"Karine L Silva, Diego Hortkoff, Gabriele Gorte, Fernanda Gumy, Michael W Favoreto, Alessandro D Loguercio, Paulo V Farago","doi":"10.1007/s10266-024-01022-z","DOIUrl":"https://doi.org/10.1007/s10266-024-01022-z","url":null,"abstract":"<p><p>This study assessed the hydrogen peroxide (HP) diffusion into the pulp chamber, bleaching efficacy (BE), surface roughness (Ra), and Knoop microhardness (KHN) of an experimental bleaching gel containing a hydroxyapatite-capsaicin composite (HAp-CAP). Human premolars were randomly assigned to three groups (n = 9) based on the dental bleaching gel used (50 min; one session): only 35% HP, 35% HP + HAp-CAP, and not exposed to bleaching (negative control; NC). HP diffusion (µg/mL) into the pulp chamber was assessed using UV-Vis spectrophotometry, and BE (ΔE_ab, ΔE₀₀, and WI_D) was evaluated using a digital spectrophotometer. Human molars were used to determine Ra and KHN before and 7 days after the bleaching procedure, and for Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses. A paired t-test or t-test was used for statistical analysis (α = 0.05). A higher amount of HP into the pulp chamber and significant BE (ΔE_ab, ΔE₀₀, and WI_D) were observed for 35% HP and 35% HP + HAp-CAP compared to NC (p < 0.001), with no significant difference between them (p > 0.05). SEM and EDS demonstrated material deposition on the enamel surface after bleaching for 35% HP + HAp-CAP, which helped maintain the KHN (p > 0.05). Furthermore, Ra increased for both groups (p < 0.05). The 35% HP + HAp-CAP did not decrease HP diffusion into the pulp chamber, reduced KHN values, and increased Ra. Material deposition on the enamel surface was observed, and BE was effective.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of chitosan root canal medicament against cross-kingdom dual-species biofilm of Candida albicans and Enterococcus faecalis in an in vitro root-canal model.","authors":"Ornuma Srihawan, Anchana Panichuttra, Veera Lertchirakarn, Oranart Matangkasombut","doi":"10.1007/s10266-024-01024-x","DOIUrl":"https://doi.org/10.1007/s10266-024-01024-x","url":null,"abstract":"<p><p>Infection of the root canals with cross-kingdom biofilm of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) causes more inflammation and is resistant to conventional medication. This study aimed to evaluate the efficacy of chitosan paste (chitosan + propylene glycol (PG)) against this dual-species biofilm in comparison to calcium hydroxide (Ca(OH)₂) in the root canals of human teeth. Fifty extracted single-rooted human teeth were prepared and inoculated with C. albicans and E. faecalis, and incubated for 3 days for biofilm formation. Four experimental groups (N = 10 each) were applied with different root canal medications for 7 days, including (1) No medication (negative control); (2) Ca(OH)₂ + distilled water; (3) PG (vehicle control) and (4) chitosan + PG. The efficacy of root canal medicaments was assessed by determining the percentage of remaining colony-forming units (CFUs) of viable microorganisms in intracanal dentin. The dentin was collected, serially diluted, and spread on solid media for colony count. The biofilm structure in root canals at 3 days and 10 days (ie. before and after treatments) were observed with scanning electron microscopy (SEM) at 5000× and 10,000× magnifications. Data were analyzed using Welch's ANOVA and Games-Howell post-hoc test (α = 5%). The results show that Chitosan + PG group had a significantly lower percentage of remaining microorganisms when compared to PG, Ca(OH)₂, and negative control groups (P-value = 0.015, 0.005, and < 0.001, respectively). Consistently, SEM showed the lowest amount of remaining biofilm in Chitosan + PG group. Thus, Chitosan + PG paste was the most efficacious root canal medicament against dual-species biofilm of C. albicans and E. faecalis in this model.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of hinokitiol in ameliorating oral cancer: in vitro and in silico evidences.","authors":"Anitha Roy, Binoy Varghese Cheriyan, Elumalai Perumal, Kannan Rr Rengasamy, Shanmugam Anandakumar","doi":"10.1007/s10266-024-01020-1","DOIUrl":"https://doi.org/10.1007/s10266-024-01020-1","url":null,"abstract":"<p><p>The study aimed to evaluate the anticancer potential of hinokitiol in treating oral cancer by using in vitro models and examining its interaction with the Pim-1 protein through in silico methods. Hinokitiol was applied to KB-1 oral squamous carcinoma cells, where the half-maximal inhibitory concentrations (IC₅₀) was determined. Morphologic changes in treated cells were observed using phase contrast microscopy, while acridine orange/ethidium bromide (AO/EB) staining was used to assess nuclear changes and apoptosis. Flow cytometry was employed to analyze the cell-cycle progression. Given the high expression of Pim-1 in oral squamous carcinoma cells, molecular docking and simulation were performed to evaluate hinokitiol's binding affinity and stability with the Pim-1 protein. To compare its effects, hinokitiol was also tested on non-cancerous pre-adipocytes (3T3-L1), providing insights into its selective cytotoxicity between healthy and cancerous cells. Hinokitiol treatment resulted in cytotoxic effects on KB-1 oral squamous carcinoma cells, with an IC₅₀ of 30 µg/mL after 24 and 48 hs of exposure. Morphologic studies showed reduced cell population and density. In contrast, hinokitiol exhibited lower toxicity and caused fewer morphological changes in non-cancerous 3T3-L1 pre-adipocytes. Apoptosis was confirmed through acridine orange/ethidium bromide staining, while flow cytometry revealed cell-cycle arrest in the Synthesis phase (S) and Gap 2 phase/ Mitosis Phase (G2/M) phases. Molecular docking showed strong binding of hinokitiol to Pim-1, and simulations confirmed the interaction's stability. These findings suggest hinokitiol selectively targets cancer cells and effectively inhibit Pim-1, supporting its potential as an oral cancer treatment.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metronidazole-laden silk fibroin methacrylated scaffolds for managing periapical lesions.","authors":"Ashley Silverberg, Lais M Cardoso, Ana Beatriz G de Carvalho, Alexandre H Dos Reis-Prado, J Christopher Fenno, Renan Dal-Fabbro, Marco C Bottino","doi":"10.1007/s10266-024-01023-y","DOIUrl":"https://doi.org/10.1007/s10266-024-01023-y","url":null,"abstract":"<p><p>This study aimed to develop and characterize silk fibroin methacrylated/SilkMA electrospun scaffolds associated with metronidazole/MET to control infection in root-end resected periapical lesions while supporting bone regeneration. SilkMA-based formulations (10% w/v) incorporating MET (0-control; 5, 15, or 30% w/w) were electrospun into fibrous scaffolds and photocrosslinked. Scaffolds' morphology, chemical composition, swelling/degradation profiles, mechanical properties, cytocompatibility with alveolar bone-derived mesenchymal stem cells/aBMSCs and stem cells from apical papilla/SCAPs, anti-inflammatory potential, and antibacterial efficacy (direct contact assay against Aggregatibacter actinomycetemcomitans/Aa and Fusobacterium nucleatum/Fn; Aa biofilm model) were assessed. Statistical analysis was conducted using a significance level of 5%. Morphological analysis revealed that MET content influenced fiber diameters post-crosslinking, while the chemical composition analysis confirmed MET integration within the scaffolds. 30%MET-laden scaffolds demonstrated reduced swelling capacity compared to SilkMA/control scaffolds, while complete degradation was observed after 42 days for the formulated scaffolds. Mechanical testing indicated enhanced stiffness and tensile strength in 30%MET-laden scaffolds compared to SilkMA/control (p < 0.05). Cytocompatibility evaluations showed non-cytotoxic effects across all formulations for aBMSCs and SCAPs. Anti-inflammatory assays demonstrated decreased pro-inflammatory cytokine interleukin-6 synthesis by aBMSCs treated with SilkMA + MET30% and Escherichia coli LPS, comparable to negative control (p > 0.05). Antibacterial efficacy assays revealed significant inhibition of Aa and Fn, with 30%MET-laden scaffolds demonstrating biofilm inhibition against Aa (p < 0.05). These findings underscore the potential of SilkMA scaffolds laden with MET as a promising strategy for managing periapical lesions, offering enhanced structural support, antimicrobial properties, and biocompatibility crucial for effective tissue regeneration and infection control after endodontic surgery.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zirconia in fixed prosthodontics: a review of the literature.","authors":"Shoko Miura, Takafumi Fujita, Masanori Fujisawa","doi":"10.1007/s10266-024-01019-8","DOIUrl":"https://doi.org/10.1007/s10266-024-01019-8","url":null,"abstract":"<p><p>The purpose of this narrative review is to organize information and knowledge about the properties of the clinical evidence of zirconia-applied dental restorations and to provide relevant information to establish application methods to obtain long-term clinical prognosis of zirconia-applied dental prostheses. From PubMed and manual searches, found \"zirconia,\" \"crown,\" \"survival,\" and \"success\" keywords were searched. Case reports, case series, reviews, abstracts, in vitro studies, and observational studies were excluded. Additionally, implant superstructures not supported by the periodontal ligament and partially covered crowns were excluded. A total of 42 studies were identified. According to the studies in this review, the most common complication of porcelain-layered zirconia restorations is porcelain fracture, but the long-term prognosis is stable. However, complications of monolithic zirconia restorations include crown fractures and fractures of the abutment tooth and root, and long-term clinical evidence is not available yet. Additionally, bruxism can be a complication factor with both types of restorations, so it is important to carefully examine the oral habits, including bruxism, before treatment. Therefore, the success of zirconia-based restorations in clinical practice requires a careful approach in addition to accurate knowledge of the zirconia material and identification of applicable cases.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Studies on the antifungal effects of Hinokitiol on Candida albicans: inhibition of germ tube formation and synergistic pharmacological effects of miconazole.","authors":"Nobuchika Takeuchi, Kayoko Fukui, Kenjirou Nakamura, Akira Tanaka","doi":"10.1007/s10266-024-01015-y","DOIUrl":"https://doi.org/10.1007/s10266-024-01015-y","url":null,"abstract":"","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a novel irrigation regimen and root canal filling material versus conventional endodontic protocols on dentin wettability.","authors":"Tan Fırat Eyüboğlu, Efe Armağan, Ayşe Nur Temel, Mutlu Özcan","doi":"10.1007/s10266-024-01017-w","DOIUrl":"https://doi.org/10.1007/s10266-024-01017-w","url":null,"abstract":"<p><p>The purpose of this study was to compare the effect of different irrigation regimens on the dentin wettability of root canal sealers. The occlusal surfaces of human molar teeth (N = 90) were removed, polished, and divided into three groups (n = 30) based on the irrigation regimen: control group (SAL), standard irrigation group (NES), and test group (ODC). Each group was randomly divided into three subgroups according to the root canal sealer: AH Plus (AHP), Total Fill BC Sealer HiFlow (TOT), and OdneFill (ODF). The contact angle was determined using the sessile drop technique. The pendant droplet method assessed the work of adhesion. Surface roughness was measured using atomic force microscopy. Data were analyzed using non-parametric tests (p < .05). ODC-ODF showed significantly lower contact angle (34.80°) than ODC-AHP (66.53°, p < .001) and ODC-TOT (85.72°, p < .001). In NES, no significant difference was found between ODF and AHP (p > .05) while both groups presented significantly lower results than TOT (p < .001, p < .001, respectively). In SAL, the difference between TOT (92.84°) and AHP (83.24°) was insignificant (p > .05), whereas ODF presented significantly lower results compared to both (p < .001, p < .001, respectively). Surface roughness was lowest in ODC (31.54 nm), followed by SAL (62.30 nm) and NES (72.02 nms) which was significantly (p = 0.006) higher than ODC, whereas the difference between SAL and NES was insignificant (p > .05). This study highlights the wettability of a new root canal sealer and the effect of a new irrigation activation system on the wettability of different root canal sealers. OdneFill showed superior wettability compared to other sealers while exhibiting a synergistic effect with OdneClean.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}