Odontology最新文献

{"title":"Experimental in vitro simulation of the impact of e-cigarette vapors on enamel and dentin.","authors":"Davi Ariel Nobuo Bepu, Alice Corrêa Silva-Sousa, Fabiane Carneiro Lopes-Olhê, Débora Fernandes Costa Guedes, André Luís Faria-E-Silva, Jardel Francisco Mazzi-Chaves, Manoel Damião Sousa-Neto, Aline Evangelista Souza-Gabriel","doi":"10.1007/s10266-025-01222-1","DOIUrl":"https://doi.org/10.1007/s10266-025-01222-1","url":null,"abstract":"<p><p>The aim of this study was to evaluate changes in tooth color and the bond strength of resin to enamel and dentin after exposure to electronic cigarette (e-cigarette) vapor in an in vitro vaping model, as well as to analyze the chemical composition of the materials present in the vapor. A device with a vacuum pump simulated vaping. Eighty dental slabs (40 dentin and 40 enamel) and were randomly divided into two groups. Half received e-cigarette exposure, and the other remained without vaping (control). Color changes were measured using a spectrophotometer (CIELAB). Composite cylinders were built on substrates using etch-and-rinse or self-etch strategies and subjected to loading tests. Gas chromatography-mass spectrometry (GC-MS) analyzed the organic compound of the e-liquid, while inductively coupled plasma mass spectrometry (ICP-MS) assessed the metal content. Statistical analysis was conducted using MANOVA and ANOVA (α = 0.05), and CIEDE2000 formula. E-cigarette exposure darkened (L*) and yellowed (b*) enamel and dentin. Bond strength in dentin decreased for both adhesion strategies, and in enamel using the etch-and-rinse adhesive. GC-MS identified 72 different volatile compounds, whereas ICP-MS detected 26 distinct metals. Among metals, eight exceeded the WHO (World Health Organization) tolerance limits. E-cigarette exposure altered the color of substrates and reduced resin bond strength in dentin for both adhesives, and in enamel restored with the etch-and-rinse technique. E-liquid had toxic organic compounds and metals. Exposure to e-cigarette can cause tooth discoloration and weaken bonding to dental tissues. Toxic volatile organic and metallic compounds present in vapor can adversely affect oral health.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel standardized method for inducing medication-related osteonecrosis of the jaw in rats and precise quantitative assessment of pathological outcomes.","authors":"Daichi Hanada, Mai Mochizuki, Taka Nakahara, Akira Tanaka","doi":"10.1007/s10266-025-01076-7","DOIUrl":"10.1007/s10266-025-01076-7","url":null,"abstract":"<p><p>Medication-related osteonecrosis of the jaw (MRONJ) is a serious complication of antiresorptive therapies, characterized by delayed healing, bone necrosis, and infection following dental procedures. Progress in the understanding of its pathophysiology has been hindered by the lack of standardized animal models. Existing models involving first molar or multiple molar extractions disrupt anatomic landmarks and face technical challenges, such as retained root fragments and inconsistent surgical procedures, which reduce reproducibility. To address these limitations, we developed a novel rat MRONJ model using maxillary second molar extraction combined with standardized palatal gingiva resection. By preserving the adjacent first and third molars, anatomic landmarks were retained, enabling precise and reproducible evaluations. The modified extraction technique incorporating wedge insertion improved the success rate and minimized root fractures. Notably, our findings revealed that suppressed bone metabolism in the MRONJ model inhibited natural tooth movement observed in the control group, highlighting a unique pathologic hallmark of MRONJ. The model effectively reproduced MRONJ-specific features, including persistent bone exposure, impaired bone healing, necrotic bone formation, and inflammation. Three-dimensional micro-computed tomography and histologic analyses provided robust and quantitative assessments of bone pathology. By integrating anatomic standardization and precise quantitative assessments, this model addresses the key limitations of previous approaches. It also provides a reliable platform for investigating the pathogenesis of MRONJ and for assessing preventive and therapeutic strategies. This approach contributes to translational research and holds promise for improving clinical outcomes.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1415-1426"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA HOTTIP as a potential biomarker of chronic periodontitis and its role in inflammatory responses.","authors":"Jing Guo, Ying He","doi":"10.1007/s10266-025-01094-5","DOIUrl":"10.1007/s10266-025-01094-5","url":null,"abstract":"<p><p>Chronic periodontitis (CP) is a prevalent oral condition that can elicit a broad spectrum of inflammatory responses. This study investigates the impact of lncRNA HOTTIP on inflammatory responses of CP via targeting and regulating miR-205-5p. This study involved 117 CP patients and 101 controls. The expression levels of HOTTIP and miR-101-3p in the gingival crevicular fluid (GCF) of CP patients were quantified using qPCR. The levels of inflammatory factors IL-1β, IL-6, IL-10, and TNF-α, were measured through ELISA. ROC analysis was conducted to evaluate the diagnostic efficacy. The LPS-induced hPDLFs injury model was established to investigate the effects of HOTTIP silencing, as well as the concurrent inhibition of HOTTIP and miR-101-3p expression, on cellular functionality and inflammatory responses. HOTTIP was elevated in the GCF of CP patients, whereas miR-101-3p was reduced (P < 0.001). HOTTIP exhibited a positive correlation with inflammatory markers and periodontal indicators (r > 0, P < 0.01). HOTTIP possessed the potential to serve a specific biomarker for the auxiliary diagnosis of CP (AUC = 0.967, P < 0.001), as well as a critical parameter for assessing the periodontal condition of patients. In LPS-induced hPDLFs model, the silencing of HOTTIP was found to enhance cell proliferation (P < 0.01), reduce apoptosis (P < 0.001), and the release of inflammatory factors (P < 0.05). However, the simultaneous inhibition of both HOTTIP and miR-101-3p, this inhibitory effect on inflammatory response was counteracted (P < 0.05). HOTTIP regulated cellular function and the release of inflammatory factors via miR-101-3p, thereby exacerbating the inflammatory response with CP patients. HOTTIP is anticipated to emerge as a promising biomarker for the diagnosis of CP.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1678-1688"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monomer release, cytotoxicity, and surface roughness of temporary fixed prosthetic materials produced by digital and conventional methods.","authors":"Zeynep Sahin, Deniz Ozkan Vardar, Ekin Erdogmus, Semih Calamak, Belma Koçer Gumusel","doi":"10.1007/s10266-025-01091-8","DOIUrl":"10.1007/s10266-025-01091-8","url":null,"abstract":"<p><p>This study compared surface roughness, monomer release, and, cytotoxicity of temporary fixed prosthetic materials manufactured using the conventional, CAD/CAM milling and 3D printing methods. Disc-shaped samples (2 mm height, 5 mm diameter) were prepared from four materials [polyethyl methacrylate/polymethyl methacrylate (Dentalon Plus-DP), bis-acrylic composite resin (Protemp 4-PT), polymethyl methacrylate CAD/CAM disc (On Dent), and methacrylate-based resin (QuraCROWN Temp)]. Surface roughness was measured with a profilometer; scanning electron microscopy (SEM) was used for surface characterization. Following 24, 72, and 120 h of artificial saliva incubation for the samples, the obtained extracts were evaluated for cytotoxicity by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test in the mouse fibroblast cell. Monomer release from the test samples was analyzed by High‑Performance Liquid Chromatography. Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) was performed to evaluate the chemical composition of artificial saliva extracts. Cell viability was assessed by one-way ANOVA, and surface roughness by Kruskal-Wallis and Mann-Whitney U tests. No monomer was detected in artificial saliva for any materials. The FTIR spectroscopy of the extracts did not show any peaks corresponding to these monomer or polymer structures, indicating that no residual monomer or polymer was released into the artificial saliva after exposure to artificial saliva. 3D-printed materials were significantly more cytotoxic than the other three test materials at all time points and dilutions (p < 0.05). The highest cell viability rates were detected in CAD/CAM milling (99.43 ± 3.79) at 24 h and PT materials (100.47 ± 5.31) at 72 h for 1:8 dilution. At 1:4 dilution, except for the DP-3D printing test groups, the other groups show similar cell viability rates with the control group (p > 0.05). Digitally manufactured materials had lower roughness than conventionally produced ones (p < 0.05). CAD/CAM milling and PT materials were the most biocompatible, while 3D-printed material was found to be cytotoxic. CAD/CAM milling and PT materials may offer safe and effective options for temporary prosthetic restorations. Although DP showed acceptable results, it was less effective than CAD/CAM milling and PT materials. Due to their cytotoxicity, 3D-printed materials require further investigation before clinical use.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1643-1658"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical assessment of dental color during dehydration and rehydration by various dental photography techniques.","authors":"Cigdem Haciali, Bora Korkut, Funda Yanikoglu","doi":"10.1007/s10266-025-01081-w","DOIUrl":"10.1007/s10266-025-01081-w","url":null,"abstract":"<p><p>Dental color selection is an important clinical challenge in restorative dentistry due to the alterations related to dehydration and rehydration. This clinical study aimed to quantitatively monitor the color changes between different levels of tooth dehydration and rehydration using different dental photography techniques. Color assessments of the right central incisor were performed using a hybrid-type clinical spectrophotometer (RayPlicker; RP) as control, a dental photography kit (DP), and a mobile dental photography kit (MDP). Cross-polarization filter (CP) and white balance card (WB) were also used in combinations with DP and MDP. Therefore, the effectiveness of eight different photography techniques (DP, DP-WB, DP-CP, DP-WB-CP, MDP, MDP-WB, MDP-CP, and MDP-WB-CP) were investigated. The color measurements during dehydration (at initial, 1, 2, 3, 5, and 15 min) and rehydration (at 30, 60 min, and 24 h) were performed with all techniques. Color changes (∆E₀₀*) were calculated according to the CIEDE2000 formula by comparing the outcomes with the initial values. Regarding the dehydration values by RP, the color changes until the 3rd min were imperceptible (∆E < 0.8) and significantly lower than the 5th min (p < 0.001). The changes were perceptible but acceptable at the 5th min, while it was not even acceptable (∆E ≥ 1.8) at the 15th min. Regarding the rehydration values by RP, the color changes were perceptible and significantly higher at the 30th and 60th min than at the 24th h (p < 0.001). The color changes for 70% of the teeth were below 0.8 at the 24th h. Very high agreements were observed between the assessments by DP/CP, DP/WB/CP, MDP/CP, and MDP/WB/CP techniques, and the RP (p < 0.001 for each assessment method). The clinical color change was considered imperceptible until 3 min of dehydration. However, it became perceptible after 5 min of dehydration and not clinically acceptable after 15 min. Following 15 min of dehydration, the natural tooth color may not be completely reversed even after rehydrating for 24 h. CP filters were considered essential tools when using dental photography or mobile dental photography devices for dental color assessment.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1558-1572"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of confocal laser scanning microscopy to evaluate the metal ion removal and destabilization of Enterococcus faecalis biofilms by EDTA and etidronic acid.","authors":"Talita Tartari, Carlos Estrela, Larissa Barbosa Borges de Araújo, Márcia Sirlene Zardin Graeff, Flaviana Bombarda de Andrade, Marco Antonio Hungaro Duarte","doi":"10.1007/s10266-025-01082-9","DOIUrl":"10.1007/s10266-025-01082-9","url":null,"abstract":"<p><p>Chelating substances bind to metals, forming stable complexes, rendering these essential ions unavailable for microbial metabolism and community stability in biofilms. This action can contribute to the disinfection in endodontic treatments. Through confocal laser scanning microscopy (CLSM), this study quantified the metal ion removal by chelating agents in Enterococcus faecalis biofilms and assessed the impact on community stability. E. faecalis biofilms were grown for 21 days on acrylic coverslips, which the following were immersed in (n = 10): G1) saline solution (control, 5 min); G2) 17% ethylenediaminetetraacetic acid (EDTA, 3 min); and G3) 9% etidronic acid (Dual Rinse HEDP, 5 min). Next, they were stained with Phen Green FL to identify metals, 4',6-diamidino-2-phenylindole (DAPI) to highlight bacterial DNA, and propidium iodide (PI) to mark nucleic acid in cells with damaged membranes. Images were captured (4/sample) by CLSM and analyzed by the software Leica Application Suite X. The individual and total volume of the biofilm-stained components (µm³) and their individual percentages in the biofilms were analyzed using one-way ANOVA with Tukey tests (α < 0.05). EDTA caused a higher metal removal (P < 0.05) that potentially destabilized biofilms, causing detachment of bacterial cells. Consequently, EDTA significantly reduced the total cubic volume of biofilms compared to other irrigants (P < 0.05), while control and etidronic acid groups exhibited similarity (P > 0.05). However, the percentages of nucleic acid and metals remained constant in all treatments (P > 0.05). In conclusion, strong chelating solutions, such as EDTA, can remove substantial amounts of metals from biofilms and affect the community stability.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1573-1581"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotype and distribution of adhesion genes in Streptococcus mutans isolated from people with Parkinson's disease.","authors":"Laura Donnet, Olivier Claisse, Johan Samot","doi":"10.1007/s10266-025-01078-5","DOIUrl":"10.1007/s10266-025-01078-5","url":null,"abstract":"<p><p>The delicate balance of the oral cavity is disrupted by changes in hygiene and diet, leading to caries. The virulence of Streptococcus mutans, a key caries pathogen, is partly explained by its rhamnose-glucose polysaccharide (RGP) and its sucrose-dependent and -independent adhesion mechanisms. Given their role in comorbidities, this study investigates the diversity of S. mutans through analysis of RGP and sucrose-independent adhesion proteins (SpaP, WapA, Cnm, Cbm) in Parkinson's disease patients. In the PARKIDENT clinical trial, strains were isolated from saliva samples of Parkinson's patients at baseline and during a follow-up visit. Strains were preanalyzed by multiplex PCR for serotype and collagen-binding proteins, followed by high-throughput sequencing and bioinformatics analysis using RAST® and BLAST®. Phylogenetic analyses and protein modeling were also conducted. Of 40 patients in the Parkident clinical trial, only 24 had Streptococcus mutans strains isolable from salivary swabs. Serotyping revealed that over 80% of the 44 strains isolated were serotype c, in line with prevalence data in the literature. Furthermore, only SpaP types A and B were identified in the strains. The low variability observed for the WapA protein underlines its functional importance. Finally, the cbm gene was not found in any strain, and only 5 strains possessed the cnm gene, all of them serotype c. The study of these 44 Streptococcus mutans strains showed characteristics similar to other populations. Serotype c predominates, with minimal peptide sequence variability in adhesion proteins (SpaP, WapA). Collagen-binding proteins Cnm and Cbm are rare or absent. Further research is essential to better characterize strain distribution in the Parkinson's-affected population due to S. mutans' growing role in extra-oral pathologies. Trial registry: ClinicalTrials.gov ID NCT03827551, First submitted: 2019-01-25.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1520-1532"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of CBCT with blooming artifact reduction filter on the measurements of dentin thickness in root canals with radiodense materials: an in vitro study.","authors":"Diego Leonardo de Souza, Luiz Carlos de Lima Dias Junior, Elimar Fernando Bauer, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garcia, Eduardo Antunes Bortoluzzi","doi":"10.1007/s10266-025-01099-0","DOIUrl":"10.1007/s10266-025-01099-0","url":null,"abstract":"<p><p>The precision of dentin thickness evaluations in the presence of artifacts is vital, as inaccuracies may increase the risk of over-preparation and consequent structural damage to the tooth. The purpose of this in vitro study was to evaluate the accuracy of residual dentin thickness measurements in CBCT images of teeth with high-density intracanal materials, utilizing the blooming artifact reduction (BAR) filter. Twelve single-rooted human teeth were selected. Axial cuts were performed, and the root thickness was measured at the buccal, lingual, mesial, and distal surfaces, which represented the reference standard. CBCT scans were acquired of each specimen in 5 different settings: empty root canals (ERC), gutta-percha cone (GPC), gutta-percha and endodontic sealer (GPC + S), fiberglass post (FGP), and metal post (MP). Measurements were performed before and after the application of the BAR filter in the e-vol DX software. ANOVA with the Dunnet's post hoc test was used for comparison between measurements in CBCT images and the reference standard. The BAR filter did not influence the measurements in teeth with ERC, GPC, and FGP (p > 0.05). In teeth with GPC + S, the measurements with the BAR filter had no difference from the reference standard (p < 0.05). CBCT images measurements were comparable to the reference standard in the ERC, GPC, and FGP groups. BAR filter was restricted to the GPC + S group in the cervical third, and the MP group in the apical third. In root canals with metal posts, the overall dentin thickness was significantly underestimated in all CBCT images within the cervical and middle thirds.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1712-1721"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of air-thinning a two-step adhesive layer on bond strength to tooth substrates.","authors":"Yuko Nagasawa, Yoshikazu Eda, Shigeki Matsumoto, Hiroshi Nakajima, Yasushi Hibino","doi":"10.1007/s10266-025-01098-1","DOIUrl":"10.1007/s10266-025-01098-1","url":null,"abstract":"<p><p>This study investigated the effect of air-thinning to a two-step adhesive (G2-BOND Universal) on the microtensile bond strength (µTBS) between bovine teeth (enamel, dentin) and a resin composite (G-ænial A'CHORD). The adhesive was applied with or without air-blowing before light curing on the bovine enamel and dentin surfaces finished using 320-grit SiC paper. After resin composite buildup, µTBS specimens (1.0 mm × 1.0 mm cross-sectional area) were fabricated. The µTBS values were determined 24 h, 4 weeks, and 6 months after the specimen preparation. Furthermore, the adhesive layer thicknesses were measured on the SEM photographs of the specimens' fractured site after the µTBS test. Except for the enamel specimens at 6 months, no significant differences were observed in µTBS determined at any time periods between specimens with and without air-blowing (p > 0.05). µTBS values in air-blown specimens increased from 24 h to 4 weeks, and the values in non-air-blown specimens were greater at 6 months than at 24 h. Specimens subjected to air-blowing showed significantly thinner adhesive layer than those without at any time periods (p < 0.05). Although air-blowing reduced adhesive layer thickness, it did not affect the bond strength except for the enamel specimens at 6 months.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1436-1446"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of irrigation solutions to dental-derived mesenchymal stem cells in regenerative endodontic procedure: a systematic review of in vitro studies.","authors":"Lim Hui Ying, Mariam Abdullah, Natrah Ahmad Fuad, Lim Ghee Seong, Noor Hayati Azami, Nora Sakina Mohd Noor, Myrna Nurlatifah Zakaria","doi":"10.1007/s10266-025-01087-4","DOIUrl":"10.1007/s10266-025-01087-4","url":null,"abstract":"<p><p>Regenerative endodontic procedures (REPs) offer an alternative to apexification in necrotic immature permanent teeth, promoting continued root development and dentinal wall thickening. Success in REPs requires effective disinfection and the survival of dental-derived mesenchymal stem cells (DMSCs), such as dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs), and periodontal ligament stem cells (PDLSCs). This review investigates the biocompatibility of irrigation solutions, including sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX), on DMSCs. Following PRISMA guidelines, a comprehensive search was conducted in PubMed, Scopus, Web of Science, Cochrane, and SciELO, with the last update on March 4, 2024. Studies from January 2008 to April 2024 assessing viability, proliferation, migration, differentiation, and mineralization of DMSCs treated with NaOCl, EDTA, and CHX were included. The papers were selected using PICOS criteria and quality was assessed using the PRILE checklist and risk of bias with the Quality Assessment Tool for In Vitro Studies. Of 738 studies identified, 15 met inclusion criteria. The findings suggest that NaOCl and CHX exhibit lower biocompatibility towards DMSCs compared to EDTA. NaOCl and CHX are cytotoxic to DMSCs, while EDTA demonstrates favorable biocompatibility, promoting osteogenic differentiation and mineralization. This highlights potential implications for irrigant selection in regenerative procedures, as appropriate irrigants may enhance cellular survival and improve clinical outcomes.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":"1315-1329"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}