Odontology最新文献

{"title":"HNF1A-AS1 promotes oral squamous cell carcinoma progression via regulating miR-138/CDK6 pathway.","authors":"Bingxin Mei, Zhimei Zeng, Qinmin Xia, Ming Liu, Li Lei","doi":"10.1007/s10266-025-01057-w","DOIUrl":"https://doi.org/10.1007/s10266-025-01057-w","url":null,"abstract":"<p><p>The action and the latent mechanism of HNF1A-AS1 in oral squamous cell carcinoma (OSCC) development were probed. Levels of HNF1A-AS1, microRNA-138 (miR-138) and Cyclin-dependent kinase 6 (CDK6) were examined. In vitro assays were conducted using SCC-4 and SCC15 cells derived from a human SCC of the tongue of a 55-year-old male. In vivo assay was performed by establishing OSCC mouse models. An elevated HNF1A-AS1 was detected in OSCC, and down-expressed HNF1A-AS1 inhibited migration and invasion, and promoted apoptosis in OSCC cells in vitro. HNF1A-AS1 targeted miR-138 to positively regulate the expression of CDK6, a target of miR-138. Knockdown of miR-138 attenuated the action of HNF1A-AS1 silencing on OSCC cell malignant phenotypes. Besides that, overexpression of CDK6 weakened miR-138-mediated anti-cancer functions. Moreover, HNF1A-AS1 knockdown restrained OSCC growth in nude mice. HNF1A-AS1 promoted OSCC tumorigenesis via miR-138/CDK6 pathway, indicating the potential molecular contribution of HNF1A-AS1 on OSCC pathogenesis.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can pigments of different natures interfere with the cytotoxicity from in-office bleaching?","authors":"Rafael Antonio de Oliveira Ribeiro, Beatriz Voss Martins, Marlon Ferreira Dias, Victória Peruchi, Caroline Anselmi, Igor Paulino Mendes Soares, Josimeri Hebling, Vanessa Cavalli, Carlos Alberto de Souza Costa","doi":"10.1007/s10266-025-01068-7","DOIUrl":"https://doi.org/10.1007/s10266-025-01068-7","url":null,"abstract":"<p><strong>Objective: </strong>To assess the influence of tooth stained with different colored solutions on the cytotoxicity of a 35% H₂O₂-bleaching gel.</p><p><strong>Materials and methods: </strong>The established groups were (n = 8): NC- without treatment (negative control); PC- 35% H₂O₂ (positive control); CO- coffee + 35% H₂O₂; BT- black tea + 35% H₂O₂; WI- wine + 35% H₂O₂; COBTWI- coffee/black tea/wine + 35% H₂O₂. Standardized enamel/dentin disks were submitted to staining protocols, adapted to artificial pulp chambers, and bleaching was performed. The chromatic alterations (ΔE_00, ΔWI_D) were assessed after bleaching and the extracts (culture medium + diffused gel components) were collected and applied to odontoblast-like cells. The amount of diffused H₂O₂, cell viability, and oxidative stress were evaluated and data were submitted to ANOVA/Tukey; p < 0.05).</p><p><strong>Results: </strong>Thirty five% H₂O₂ effectively bleached the stained specimens, regardless of the staining solution used (CO, BT_, WI, and COBTWI), and no differences were found among the stained protocols (p > 0.05). All staining protocols reduced H₂O₂ trans-amelodentinal diffusion (p > 0.05), decreasing bleaching cytotoxicity in comparison to PC (p < 0.05).</p><p><strong>Conclusion: </strong>Irrespective of the nature of the pigment used for staining enamel and dentin, the gel with 35% H₂O₂ effectively bleached the tooth structure. The presence of pigments in both dental tissues, regardless their nature, reduced the trans-amelodentinal diffusion of H₂O₂ and consequently the cytotoxicity of the bleaching gel.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metrics for prosthetic cervical margin integrity assessment after dental CAD/CAM milling: a critical analysis from engineering viewpoint.","authors":"Nicolas Lebon, Laurent Tapie","doi":"10.1007/s10266-025-01066-9","DOIUrl":"https://doi.org/10.1007/s10266-025-01066-9","url":null,"abstract":"<p><p>Dental prostheses have significantly evolved due to advances in Computer Aided Design and Manufacturing (CAD/CAM) technology. CAD/CAM systems provide a variety of biomaterials like ceramics, Polymer-Infiltrated Ceramic Network (PICN), and composites, which are preferred for their mechanical and aesthetic properties. However, ceramics, despite their popularity, are brittle and prone to chipping during the machining process, impacting the prosthesis's clinical functions, aesthetics, biological integrity, and mechanical performance. Chipping, especially at thin cervical margin, can cause visible defects, poor sealing, and bacterial growth, reducing prosthesis lifespan. Milling factors influence cervical margin integrity. Chipping assessment involves understanding biomaterial mechanical and machinability characteristics regarding dimensional characterization of milled prosthesis shape. Thus, different type of metrics, based on biomaterial properties or dimensional measurement can be used to assess chipping phenomenon for milled dental ceramics. These metrics are both, based on experimental studies found in literature, and proposed by this paper authors to fill the existing lacks. The brittleness index, based on the ratio between hardness and fracture toughness, predicts susceptibility to chipping after milling. Unidirectional dimensional metrics like the Chipping Factor and weighted Chipping Factor characterize the chipping ratio of the cervical margin. Advanced 2D and 3D metrics, including chip projected area, total weighted chip projected area, and Surface Aspect Ratio, offer more detailed assessments. 3D analysis involves comparing scanned files with CAD models to compute chipped volumes. The aim of this paper is to propose a critical analysis from an engineering viewpoint on metrics used to assess cervical margin integrity for milled dental prosthesis.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exacerbating effects of Western dietary habits on experimentally induced periodontitis in rats.","authors":"Hatem Abuohashish, Omar Omar, Nabras Alrayes, Norah AlQahtani, Muhaseena Muhamood, Hussain Alhawaj, Taleb Alkhamis, Khalid Almas","doi":"10.1007/s10266-025-01067-8","DOIUrl":"https://doi.org/10.1007/s10266-025-01067-8","url":null,"abstract":"<p><p>This study explored the impact of western diet (WD) on a ligature-induced periodontitis (PD) model. After either control diet (CD) or WD feeding for 16 weeks, male Wistar rats were allocated in six groups (n = 6). The first and second groups had no PD. The third and fourth groups had ligature-induced PD for 10 days, while the fifth and sixth groups had ligature-induced PD for 10 days, followed by ligature removal healing period for another 10 days. The CD contained 13.71% protein, 75.98% carbohydrate, and 10.31% fat, though WD composed of 14.7% protein, 40.7% carbohydrate, and 44.6% fat. After clinical evaluation, the maxillary alveolar bone and gingival tissues were collected for morphometric, microstructural, histological, and gene expression analyses. There were significant increases in the gingival bleeding index, periodontal probing depth, and tooth mobility in WD animals with PD and in the healing groups. The WD groups had a greater alveolar crest height, indicating greater bone resorption. Disruption of the bone microarchitecture by PD was exacerbated in WD-fed animals. The histological evaluation demonstrated a greater extent of gingival inflammation in the PD groups. The Tnf, Il6, Ctsk, and Tnfsf11/RANKL gene expression levels were increased in the WD groups, while the Bglap and Hif1a gene expression levels were decreased in the WD groups. Findings of the study are compelling preclinical evidence that WD deteriorates periodontal health and exacerbates periodontal disease and alveolar bone loss in experimental animals. Future clinical research is warranted to translate these preclinical findings.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of irrigation needle design on cleaning efficiency in 3D mandibular molar models.","authors":"Alessandra Baasch, Ana Ramírez-Muñoz, Natalia Navarrete, Mar Navarro-Candel, Juan M Koury-González, Osyanel Esteves-Nieves, Gaya C S Vieira, Alejandro R Pérez","doi":"10.1007/s10266-025-01064-x","DOIUrl":"https://doi.org/10.1007/s10266-025-01064-x","url":null,"abstract":"<p><p>This study aimed to evaluate the effectiveness of various endodontic irrigation needles in removing residual tissue from root canals, focusing on the apical, middle, and coronal thirds. Forty 3D resin replicas of mandibular molars (n = 10/group) were divided into four distinct needle types: an open-ended needle, a closed-ended and lateral-vented needle, a closed-ended and double lateral-vented needle, and a polyethylene irriFlex needle with a double lateral outlets. A 30G NaviTip needle uniformly filled the canal system, delivering the artificial biofilm mixture formulated with a hydrogel. Then, the pre- and post-irrigation images were assessed quantitatively to measure tissue removal and qualitatively to determine the presence or absence of tissue remnants. The open-ended needle significantly (p < 0.05) removed tissue across the entire canal length. Open-ended and polyethylene irriFlex needles were more effective in the apical region than closed-ended needles (p < 0.05). Specifically, in the apical third, the percentages of remaining tissue were 23.7% for the open-ended and 26.8% for the irriFlex needle, compared to 53.9% and 44.9% for the closed-ended needles. The open-ended needle showed superior efficacy in the middle and coronal regions (p < 0.01). Qualitatively, only one sample was completely free of tissue in the entire root canal and one in the apical third. The open-ended needle demonstrated superior cleaning efficacy in the root canal, particularly in the middle and coronal regions, while the irriFlex needle performed better in the apical region than closed-ended needles. These results showed the importance of needle type in effective root canal irrigation. This study indicates that needle type significantly affects tissue removal in endodontic irrigation; open-ended and polyethylene needles are most effective in apical canals.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of irrigation activation methods in removal of bioceramic-based sealer in retreatment.","authors":"Büşra Nur Cıkrık, Selen İnce Yusufoğlu","doi":"10.1007/s10266-025-01054-z","DOIUrl":"https://doi.org/10.1007/s10266-025-01054-z","url":null,"abstract":"<p><p>In cases where initial root canal therapy is unsuccessful, retreatment is the first treatment choice. However, when bioceramic-based sealers have been used in the initial treatment, their complete removal can be challenging. The objective of this study was to evaluate the efficacy of three final irrigation activation methods-conventional needle irrigation, passive ultrasonic activation, and EDDY activation-using a scanning electronic microscopy (SEM) to evaluate the removal of bioceramic-based sealers after retreatment. A total of 108 single-rooted teeth were prepared using ProTaper Next rotary files. The samples were obturated with either MTAFillapex or BioSerra (n = 54) and stored for 2 weeks post-obturation. After the retreatment procedures, the teeth were divided into six groups (n = 18 per group) based on final irrigation activation method used. Group1:MTAFillapex-CNI, Group2:BioSerra-CNI, Group3:MTAFillapex-EDDY, Group4:Bioserra-EDDY, Group5:MTAFillapex-UltraX, Group6:Bioserra-UltraX. The roots were sectioned longitudinally, and the coronal, middle and apical parts were investigated under a SEM. The data were then statistically analysed. The EDDY activation technique proved significantly more effective in removing MTA Fillapex across all sections of the root canal (p < 0.001). In contrast, Ultra-X was more effective in removing Bioserra in the middle and apical sections (p = 0.003, p < 0.001). These findings suggest that activation procedures may be preferable for removing bioceramic-based root canal sealer.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a predictive model for the transition from irreversible pulpitis to periodontal-involved disease.","authors":"Jinxin Zheng, Bin Yin, Pingping Bao","doi":"10.1007/s10266-025-01061-0","DOIUrl":"https://doi.org/10.1007/s10266-025-01061-0","url":null,"abstract":"<p><p>Irreversible pulpitis can develop into pulp necrosis, causing apical periodontitis (AP). The progression of apical periodontitis is always accompanied by periodontal disease. Many similar inflammatory response pathways are involved in both apical periodontitis (AP) and periodontal disease. The infiltration of specific immune cells and the activation of immune responses are thought to play important roles in the transition from irreversible pulpitis to AP and periodontal-related diseases. This study explored immune response characteristics during the transformation of pulpitis to periodontal disease. The study also constructed a predictive model by combining known clinical and imaging indicators related to this transformation. Gene expression data related to irreversible pulpitis, apical periodontitis, and periodontal disease were extracted from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were compared and identified. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis was performed to identify immune-related pathways. Key gene signatures were screened, and a retrospective research strategy was used to collect 80 samples from patients with irreversible pulpitis. Information about pulp sensation test indicators, caries depth, root canal curvature, and inflammation indices of the patients were combined, and a logistic regression model of clinical periodontal disease was created. Receiver-operating characteristic (ROC) curve analysis was performed to evaluate the predictive performance of the model. Five genes were identified as common gene signatures during the transition from pulpitis to apical periodontitis and periodontal-involved disease. Following regression and ROC analyses revealed the expression of four genes, and the electric pulp test (EPT) value formed a predictive model with the best specificity and sensitivity in predicting the occurrence of apical periodontitis. A prognostic model was constructed and evaluated for predicting the transition from pulpitis to periodontal disease.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of bond strength and bioactivity on a prototype resin-based luting agent containing a novel bioactive monomer.","authors":"Taro Iwasaki, Kei Kubochi, Hiroki Takata, Futoshi Komine","doi":"10.1007/s10266-025-01053-0","DOIUrl":"https://doi.org/10.1007/s10266-025-01053-0","url":null,"abstract":"<p><p>A prototype luting agent based on a resin composite containing a novel bioactive monomer (a calcium salt of 4-methacryloyloxyethyl trimellitate; CMET) was developed, and its shear bond strength to tooth materials (dentin and enamel) and bioactivity was compared with those of commercially available resin-based luting systems. Extracted bovine incisors were embedded in a mold with a potting material. The labial bonding surface of the embedded tooth was wet-ground using #400 silicon carbide abrasive paper until sufficient superficial enamel or dentin was exposed. The bonding and luting agents assessed included our experimental bonding agent and luting agent, Prime&Bond Universal, Calibra Ceram, Multilink Primer A + B, Multilink Automix, Panavia V5 Tooth Primer, Panavia V5, RelyX Universal Resin Cement, SA Luting Multi, and SpeedCem Plus. The experimental luting agent was a novel material containing CMET. Shear-bond-strength testing was performed at 0 and 5000 thermocycles. To evaluate the in vitro bioactivity, specimens were immersed in 22 mL of simulated body fluid (SBF) at 37 °C for 5 days. Scanning electron microscopy combined with energy-dispersive X-ray spectroscopy was used to examine the chemical composition of the specimen surfaces after immersion in the SBF. In conclusion, the shear bond strength to dentin and enamel, and durability of the novel bonding and luting agents were similar to those of commercially available resin-based luting systems. Furthermore, the novel luting agent had bioactive potential.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-acetylcysteine antimicrobial action against endodontic pathogens-systematic review and meta-analysis.","authors":"Amjad Abu Hasna, Rayana Duarte Khoury, Gabriela Vieira Mendes, Cláudio Antonio Talge Carvalho, Eduardo Bresciani, Marcia Carneiro Valera","doi":"10.1007/s10266-025-01063-y","DOIUrl":"https://doi.org/10.1007/s10266-025-01063-y","url":null,"abstract":"<p><p>Effective root canal disinfection is crucial for the success of endodontic treatment. N-acetylcysteine (NAC), known for its antimicrobial properties, has recently been investigated as a potential endodontic irrigant or intracanal medication. This systematic review aims to assess the antimicrobial efficacy of NAC in comparison to sodium hypochlorite, chlorhexidine, and calcium hydroxide against endodontic pathogens. A comprehensive search was conducted in PubMed, Scopus, Web of Science, Cochrane Library, and LILACS databases up to April 2024, without language or date restrictions. The PICO strategy for this review were as follows: population-teeth requiring endodontic treatment; intervention-NAC used as an endodontic irrigant or intracanal medication; comparison-sodium hypochlorite, chlorhexidine, and calcium hydroxide; Outcomes: reduction in microbial load, encompassing clinical and in vitro studies. Risks of bias assessment and data extraction were conducted with two reviewers independently selecting studies, extracting data, and assessing risk of bias. A general meta-analysis was performed across all included studies, with additional meta-analyses evaluating different exposure times, NAC concentrations, control groups and evaluation methods. After removing duplicates, 9170 studies were initially identified, and seven in vitro studies were included in the systematic review, of which five were included in the meta-analysis. Data were compared using standardized mean differences within a random-effects model. No clinical studies using NAC as an antimicrobial agent were identified. The overall meta-analysis demonstrated that NAC effectively reduced Enterococcus faecalis. Further meta-analyses revealed that exposure time, NAC concentration and choice of control group significantly influenced NAC's effectiveness. NAC effectively reduced Enterococcus faecalis, showing comparable antimicrobial activity to CHX and NaOCl, especially at concentrations of 25-50 mg/mL over a 7-day exposure. Despite significant heterogeneity across studies, NAC demonstrated satisfactory antimicrobial effects in vitro. This suggests that NAC merits reconsideration as an effective intracanal medication for clinical use.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of build angle, printing layer thickness and aging on the flexural strength, precision, roughness and porosity analysis of a printed resin for provisional restorations.","authors":"Fernanda Gurgel de Gois Moreira, Marcella Guedes Pereira Gouvêa Bezerra, Larissa Mendonça de Miranda, Sarah Emille Gomes da Silva, Eugênia Livia de Andrade Dantas, Frederico Barbosa de Sousa, Mutlu Özcan, Rodrigo Othávio de Assunção E Souza","doi":"10.1007/s10266-025-01060-1","DOIUrl":"https://doi.org/10.1007/s10266-025-01060-1","url":null,"abstract":"<p><p>Evaluate the impact of printing parameters on flexural strength (σ), flexural modulus (E), precision, and surface topography characteristics of a resin used to produce provisional restorations. 450 bars for provisional restorations were printed using the SLA printing (25 × 2x2mm ISO-4049), and randomly divided into 30 groups (n = 15) according to the following factors: \"printing layer thickness\" (25 μm;50 μm;100 μm), \"Build angle\" (0°,30°,45°,60°,90°) and \"thermocycling-TC\" (with or without). Following printing, the samples underwent cleaning with isopropyl alcohol. Photopolymerization was performed for 15 min with an UV lamp. Subsequently, each bar was assessed using a digital caliper at 11 specific points in three dimensions for comparison with the STL file area for precision analysis. Half of the samples underwent thermocycling. All samples were submitted to the σ test. Data for σ (MPa), and (GPa) and precision (mm) were analyzed using 3 and 2-way ANOVA, respectively, and Tukey's post hoc test (5%). Micro-CT, 3D profilometry, and SEM were also performed and analyzed descriptively. The 90°/25μm_TC (63.0 ± 4.5) showed the highest σ, being only statistically similar to the 45°/25μm_TC (57.7 ± 3.1). For precision 0°/25 μm (-2.56 ± 0.04) expressed the greatest variation to the other experimental groups with a sample shrinkage of 25.6% compared to the STL file. The profilometry revealed that the 30º/25 μm group showed prominent peaks and valleys, presenting elevated roughness values with an average of Sa (28.25 μm). Moreover, it was observed that the groups with a 60° angle presented the lowest porosity values. A print layer thickness of 25 μm combined with a build angle of 90º and 45º resulted in higher σ and greater precision.</p>","PeriodicalId":19390,"journal":{"name":"Odontology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}