Nitric oxide : biology and chemistry最新文献

{"title":"Analytic performance characteristics of the chemical-reduction gas phase-chemiluminescence assay for the quantification of nitric oxide metabolites","authors":"Michelle Menon Miyake ,&nbsp;Ipsita Mohanty ,&nbsp;Sylvia N. Michki ,&nbsp;Edwin Tamashiro ,&nbsp;Noam A. Cohen ,&nbsp;Harry Ischiropoulos","doi":"10.1016/j.niox.2025.12.002","DOIUrl":"10.1016/j.niox.2025.12.002","url":null,"abstract":"<div><div>Nitric oxide metabolites (NOm) in biological fluids have been widely used as surrogate markers to inform changes in nitric oxide-mediated signaling, which is critical for many biological functions. One method for quantifying NOm employs chemical reduction to convert the various metabolites into nitric oxide, followed by ozone-based chemiluminescence detection. Herein, we tested and quantified the bioanalytical parameters of the reduction-chemiluminescence assay and propose conditions for optimizing the assay. The slopes of the standard curves used for quantifying the metabolites showed a 3.8 % intra-assay and 7.6 % inter-assay relative standard deviation (%RSD). An 8 % and 8.5 % RSD was measured for two separate quality control serum samples, and a Levey-Jennings plot showed that all individual values fell within 1 standard deviation of the mean of all measurements. Repeat analysis of human serum samples (n = 51) resulted in an average incurred sample reanalysis of 7.7 %, with 49 samples having repeated results above the original value, indicating no loss of NOm over the four-week period. This was further confirmed by recovery experiments, which indicated average recovery rates of 100.4 ± 8.2 % and 99.3 ± 4.3 % for two operators, respectively. ANOVA of data from two operators indicated that sample-to-sample variability was the main contributor to the total variance. Overall, our results demonstrate that the assay provides appropriate and reproducible quantification of NOm, supporting its use for both exploratory and routine laboratory applications.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"161 ","pages":"Pages 1-8"},"PeriodicalIF":3.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of S-nitroso-l-cysteine in improving cardiorenal function in eNOS-KO CKD mice","authors":"Xiaofan Wu ,&nbsp;Jiahe Zhao ,&nbsp;Zhengqi Xu ,&nbsp;Feng Jiang ,&nbsp;Moran Wang ,&nbsp;Jingwen Tao ,&nbsp;Cuntai Zhang ,&nbsp;Li Lin ,&nbsp;Sheng Li","doi":"10.1016/j.niox.2025.12.003","DOIUrl":"10.1016/j.niox.2025.12.003","url":null,"abstract":"<div><h3>Objective</h3><div>Patients with chronic kidney disease (CKD) usually exhibit high incidence and mortality rates of cardiovascular disease (CVD). Patients with CKD typically exhibit endothelial dysfunction, which represents one of the risk factors for CVD. As a signaling molecule, S-nitroso-<span>l</span>-cysteine (CSNO) plays a pivotal role in endothelial function. In this study, we explored the therapeutic value of CSNO on eNOS-KO CKD mice.</div></div><div><h3>Methods</h3><div>Male eNOS-KO mice were randomly assigned to one of three groups: control, adenine-induced CKD, and CKD with CSNO nebulization (88 ppm, 20 min/day for 6 weeks). Cardiac function was assessed via Doppler echocardiography. Fibrosis, hypertrophy, lipid accumulation, ROS production, and apoptosis were evaluated by HE, Masson, WGA, Oil Red O, DHE, and TUNEL staining, respectively.</div></div><div><h3>Results</h3><div>CSNO treatment significantly increased the kidney-to-body weight ratio compared to the eNOS-KO CKD group, and urinary creatinine levels exhibited an increasing trend, although this change was not statistically significant. Furthermore, CSNO markedly attenuated renal lipid accumulation. Echocardiography revealed that CSNO significantly enhanced left ventricular ejection fraction (LVEF) and tissue Doppler E'/A′ ratio. The E/A ratio also increased in the CSNO treatment group, but this change was not statistically significant. Moreover, CSNO alleviated cardiac hypertrophy, decreased ROS production, apoptosis, and lipid accumulation compared to the eNOS-KO CKD group.</div></div><div><h3>Conclusion</h3><div>Collectively, the findings of the present study demonstrated that CSNO improved cardiorenal function in eNOS-KO CKD mice, thereby affording a novel therapeutic approach to treat CKD patients with endothelial dysfunction.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"161 ","pages":"Pages 9-17"},"PeriodicalIF":3.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stabilizing nitric oxide: MnFe2O4@Cap-SNO nanoparticles and chitosan hydrogels for controlled therapeutic delivery","authors":"Soodabeh Gharibeh ,&nbsp;Melika JaberebnAnsari ,&nbsp;Elham Askarizadeh ,&nbsp;Azhdar Heydari","doi":"10.1016/j.niox.2025.10.008","DOIUrl":"10.1016/j.niox.2025.10.008","url":null,"abstract":"<div><div>Nitric oxide (NO) is a vital bioactive molecule, but its rapid degradation limits therapeutic applications. To enhance stability and controlled release, two novel systems were synthesized: MnFe₂O₄@Cap-SNO nanoparticles and Cs@Cap-SNO hydrogel. MnFe₂O₄@Cap-SNO, incorporating captopril (Cap) as an NO donor, demonstrated prolonged NO release for up to 16h upon light exposure, following zero-order kinetics, ensuring sustained delivery. Cs@Cap-SNO, a chitosan-based hydrogel integrating nitrosated captopril (Cap-SNO), exhibited faster NO release governed by Korsmeyer-Peppas kinetics. UV–Vis spectrophotometry confirmed successful nitrosation with distinct absorption bands at 335nm and 545nm. MnFe₂O₄@Cap-SNO displayed superior stability, outperforming Cs@Cap-SNO and free Cap-SNO in NO preservation. These findings suggest MnFe₂O₄@Cap-SNO as an effective NO-stabilizing carrier for biomedical applications, particularly intargeted drug delivery. Future studies will focus on in vitro and in vivo validation to assess therapeutic efficacy.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 1-13"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145477101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen sulfide and the physiology of “totonou”: a hypothesis on sauna-induced vascular recovery","authors":"Ryosuke Shinkai ,&nbsp;Takashi Tomita","doi":"10.1016/j.niox.2025.11.004","DOIUrl":"10.1016/j.niox.2025.11.004","url":null,"abstract":"<div><div>Sauna bathing, a traditional health practice in Finland and Japan, has been associated with reduced cardiovascular and neurological risks. Circulatory recovery, marked by a shift from sympathetic to parasympathetic dominance, underlies the relaxation state known as “totonou.” Traditionally attributed to nitric oxide (NO), this effect may also involve hydrogen sulfide (H₂S), a gaseous mediator with vasodilatory, antioxidant, and anti-inflammatory properties. We hypothesize that H₂S facilitates sauna-induced circulatory recovery and that environmental exposure may modulate this process. This perspective highlights H₂S as a novel regulator and calls for basic, clinical, and epidemiological studies to test this hypothesis.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 24-27"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prone positioning and inhaled nitric oxide in patients with acute respiratory distress syndrome: synergism or independent effects?","authors":"Stefano Spina ,&nbsp;Timothy G. Gaulton","doi":"10.1016/j.niox.2025.11.008","DOIUrl":"10.1016/j.niox.2025.11.008","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 44-46"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Detection and proteomic identification of in vivo S-nitrosylated proteins in Vibrio cholerae: A novel evidence” [Nitric Oxide 159 (2025) 63–77]","authors":"Shuddhasattwa Samaddar ,&nbsp;Surupa Chakraborty ,&nbsp;Rajib Sengupta ,&nbsp;Sanjay Ghosh","doi":"10.1016/j.niox.2025.11.003","DOIUrl":"10.1016/j.niox.2025.11.003","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Page 63"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145549741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of methemoglobin formation in high-dose inhaled nitric oxide therapy: A retrospective cohort study","authors":"Talisa Bühl ,&nbsp;Stefano Cenci ,&nbsp;Stefano Gianni ,&nbsp;Bijan Safaee Fakhr ,&nbsp;Carlo Valsecchi ,&nbsp;Binglan Yu ,&nbsp;Edward A. Bittner ,&nbsp;Lorenzo Berra","doi":"10.1016/j.niox.2025.11.006","DOIUrl":"10.1016/j.niox.2025.11.006","url":null,"abstract":"<div><h3>Background</h3><div>High-dose inhaled nitric oxide (iNO), defined as doses exceeding 20 parts per million (ppm) and reaching up to 300 ppm, has demonstrated promising antimicrobial properties. However, elevated concentrations of nitric oxide (NO) can increase the formation of methemoglobin (MetHb), which can impair oxygen transport. Understanding factors influencing MetHb formation is essential to guide safe clinical use of iNO therapy.</div></div><div><h3>Methods</h3><div>We conducted a retrospective analysis of 660 high-dose iNO treatments administered to 71 individuals between April 2020 and January 2022 under three prospective protocols at a large academic medical center. The study protocols included healthy volunteers, non-pregnant patients with COVID-19 pneumonia, and pregnant patients with COVID-19 pneumonia. MetHb levels were recorded at baseline and post-treatment using CO-oximeter. Mixed-effects modeling was used to identify predictors of MetHb formation with repeated measures.</div></div><div><h3>Results</h3><div>iNO concentration was positively associated with MetHb formation, while COVID-19 pneumonia, older age, higher baseline MetHb, and increased respiratory rate were inversely associated. No significant associations were found for sex, race, or hemoglobin concentration. Nitrogen dioxide levels remained within normal ranges, and no systemic hypotension or toxicity were observed. In tachypneic patients with COVID-19 pneumonia, high-dose iNO reduced respiratory rate.</div></div><div><h3>Conclusion</h3><div>High-dose iNO up to 300 ppm was well tolerated across diverse patient populations. MetHb formation was influenced not only by iNO doses but also by individual physiological factors. These findings support the safety of high-dose iNO and provide a basis for individualized delivery strategies.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 35-43"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic transformation of the sulfur metabolome during natto fermentation: Supersulfide omics study","authors":"Tomoaki Ida ,&nbsp;Shingo Kasamatsu ,&nbsp;Mahiro Kuryu ,&nbsp;Haruka Nitta ,&nbsp;Wakana Nagamura ,&nbsp;Hina Yoshida ,&nbsp;Ayaka Kinno ,&nbsp;Aoi Morishita ,&nbsp;Takaaki Akaike ,&nbsp;Hideshi Ihara","doi":"10.1016/j.niox.2025.11.001","DOIUrl":"10.1016/j.niox.2025.11.001","url":null,"abstract":"<div><div>Supersulfides are sulfur species with catenated sulfur atoms, such as cysteine hydropersulfide (CysSSH). Although supersulfides are biologically important metabolites owing to their unique chemical properties, their transformation in plants <em>via</em> microbial fermentation remains unknown. Natto is a traditional Japanese food prepared from soybeans fermented using <em>Bacillus subtilis</em> var. <em>natto</em> and is an excellent model for investigating this transformation. Compared to unfermented soybeans, natto contains higher supersulfide contents; however, the specific molecular changes that occur during fermentation remain unclear. Herein, we investigated the molecular profiles of supersulfides in natto using mass spectrometry-based supersulfide omics. Quantitative supersulfide profiling revealed an increase in soybean supersulfide content during fermentation using <em>B. subtilis</em> var. <em>natto</em>. However, the total sulfur content did not significantly change, suggesting that microorganisms may play a role in the biotransformation of sulfur-containing molecules into supersulfides. Furthermore, quantitative supersulfide metabolomics and untargeted polysulfide omics revealed time-dependent fermentation increases in the levels of both reduced supersulfides, including CysSSH and cysteine hydrotrisulfide, and oxidized supersulfides, such as cystine trisulfide. Moreover, high-molecular-weight supersulfides were detected. Thus, the supersulfide content significantly increased during <em>B. subtilis</em> var. <em>natto</em> fermentation. Supersulfidated proteins were not detected in natto, likely because soybean-derived proteins were degraded by <em>B. subtilis</em> var. <em>natto</em>. Conversely, supersulfide proteomics revealed the presence of various supersulfide-modified proteins in soybeans, particularly the supersulfidation of 11S glycinin. This is the first study to reveal that microbial fermentation significantly transforms the supersulfide profile in plants. Moreover, the diverse supersulfides abundantly present in natto may contribute to its health-promoting properties.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 14-23"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145458865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid fabrication of nitric oxide releasing hydroxyapatite microspheres using liquid nitrogen as an antibiotic-free alternative","authors":"Peixuan Wu ,&nbsp;Hao Zhuang ,&nbsp;Zhendong Zhao ,&nbsp;Zeyu Liu ,&nbsp;Mingle Li ,&nbsp;Tawfik Khattab ,&nbsp;Yang Zhou ,&nbsp;Yuanyuan Liu","doi":"10.1016/j.niox.2025.11.007","DOIUrl":"10.1016/j.niox.2025.11.007","url":null,"abstract":"<div><div>Bacterial infections are a major global health challenge. Although antibiotics are the dominant therapeutic approach, the problem of resistance resulting from their widespread use has led to an urgent need to develop new, safe, and effective alternative antimicrobial strategies. In this work, antimicrobial SNAP-HA microspheres with controlled release of NO were successfully prepared by loading S-nitroso-N-acetyl penicillamine (SNAP) in hydroxyapatite (HA). The loading of SNAP in SNAP-HA microspheres ranged from 1.05 % to 16.42 %. The NO release from SNAP-HA microspheres was measured by chemiluminescence, and the longest NO release time reached ∼66 h ¹H NMR also characterized the decomposition products of SNAP. In addition, the structure, thermal properties, and morphology of SNAP-HA microspheres were fully characterized. SNAP was present in HA microspheres in the amorphous form. In antibacterial studies, the SNAP-HA microspheres were able to reach 100 % of the optimal antibacterial activity against <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Staphylococcus aureus</em> (<em>S. aureus</em>). SNAP-HA microspheres are antimicrobial by releasing NO gas, which does not stimulate the development of drug-resistant strains of bacteria and is expected to be a new alternative antimicrobial product.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 47-55"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the menstrual cycle on oxidative stress, inflammation and iron status at high altitude","authors":"Guia Tagliapietra ,&nbsp;Tom Citherlet ,&nbsp;Antoine Raberin ,&nbsp;Benjamin J. Narang ,&nbsp;Giorgio Manferdelli ,&nbsp;Guido Giardini ,&nbsp;Tadej Debevec ,&nbsp;Vincent Pialoux ,&nbsp;Grégoire P. Millet","doi":"10.1016/j.niox.2025.12.001","DOIUrl":"10.1016/j.niox.2025.12.001","url":null,"abstract":"<div><div>High altitude may alter redox balance and promote inflammation. It remains unclear if ovarian hormone fluctuations influence redox status. We sought to investigate the impact of menstrual cycle (MC) phases on oxidative stress, nitric oxide metabolism, inflammation, iron biomarkers and acute mountain sickness (AMS) during high-altitude sojourns in eumenorrheic women. Venous blood samples were collected at low altitude (1224 m) and after one night at 3375 m (Rifugio Torino, inspired O₂ pressure: 96 ± 1 mmHg) during both the early follicular (EF) and mid-luteal (ML) phases. At high altitude, xanthine oxidase (XO: 0.140 ± 0.077 vs. 0.165 ± 0.084 μmol L⁻¹ min ⁻¹; <em>p</em> = 1.00), total nitrites and nitrates (NOx: 38.9 ± 10.8 vs. 32.8 ± 6.1 μmol L⁻¹; <em>p</em> = 1.00), interleukin-6 (IL-6: 17.3 ± 13.6 vs. 14.5 ± 13.2 ng mL⁻¹; <em>p</em> = 1.00) and serum iron concentration (19.7 ± 6.8 vs. 22.1 ± 4.6 μmol L⁻¹; <em>p</em> = 1.00) were not significantly different between EF and ML. However, total protein concentrations were significantly lower in EF compared to ML (75.5 ± 2.0 vs. 80.0 ± 5.1 g L⁻¹; <em>p</em> = 0.010). No significant differences were observed in Lake Louise scores (AMS) between EF and ML (2.17 ± 1.64 vs. 1.50 ± 1.83; <em>p</em> = 0.180). High-altitude exposure increased XO, IL-6 and erythropoietin levels and decreased NOx, when compared to low altitude. These findings suggest that redox balance, nitric oxide bioavailability, inflammation and iron homeostasis are not influenced by the MC at high altitude. Overall, susceptibility to AMS was similar across MC phases.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"160 ","pages":"Pages 56-62"},"PeriodicalIF":3.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145805322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}