Nitric oxide : biology and chemistry最新文献

{"title":"Paradoxical attenuation of dyslipidemia and atherosclerosis in Apoe^-/-Mpst^-/- mice.","authors":"Paraskevas Zampas, Maria Markou, Nikolaos Bertos, Stella Ravani, Andreas Papapetropoulos","doi":"10.1016/j.niox.2026.10.007","DOIUrl":"https://doi.org/10.1016/j.niox.2026.10.007","url":null,"abstract":"<p><p>Hydrogen sulfide (H₂S) is a signaling molecule that affects multiple (patho)physiological pathways. Deficiency of H₂S due to cystathionine γ-lyase (CSE) deletion has been proven to accelerate atherosclerotic plaque formation. However, the role of 3-mercaptopyruvate sulfurtransferase (MPST), a related H₂S-producing enzyme that impacts metabolism, has not been studied in atherogenic dyslipidemia. We generated a double Apoe/Mpst knockout mouse (Apoe^-/-Mpst^-/-) in the C57BL/6 background. C57Bl/6, Mpst^-/-, Apoe^-/- and Apoe^-/-Mpst^-/-mice were fed a western diet for 14 weeks, after which blood and tissues (aorta and liver) were collected; the circulating lipid profile, atherosclerotic plaque formation and expression of enzymes involved in lipid biosynthetic pathways in the liver were evaluated in the four mouse lines. H₂S production was reduced in the liver of double knockout mice, while the expression of CBS and CSE was not altered. In the serum of Apoe^-/-Mpst^-/-, we observed decreased levels of total cholesterol, low-density lipoproteins (LDL) and triglycerides (TGs) compared to Apoe^-/-; hepatic apolipoprotein B (ApoB) levels were also downregulated. However, the expression of other key enzymes that participate in cholesterol and fatty acid synthesis remained unchanged. In addition, hepatic lipid deposition was not significantly altered among genotypes. In line with the reduced circulating LDL and TGs levels, atherosclerotic lesions were found to be reduced in the aorta of Apoe^-/-Mpst^-/-. Our results indicate that MPST deletion in the Apoe^-/- dyslipidemia model leads to decreased circulating lipid levels and atherosclerosis, that correlate with a downregulation of hepatic ApoB expression.</p>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tidal Breathing Exhaled Nitric Oxide Measurement Using A Novel Chemiluminescence Analyzer: Clinical Potential in Lung Disease Diagnosis.","authors":"Ailing Song, Hongchao Fang, Huan Liu, Wenbing Zhang, Xiaobang Zhu, Xiangwei Zhang, Jing Cao, Leixin Guo, Ping Wang, Jun Zhou, Jianping Zhou","doi":"10.1016/j.niox.2026.10.005","DOIUrl":"https://doi.org/10.1016/j.niox.2026.10.005","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;In 2024, Chinese first domestically developed chemiluminescence-based exhaled nitric oxide (eNO) analyzer, eNOglow, entered the market. This innovative device utilizes a novel methodology, termed the tidal breathing eNO method (TBNO), which enables rapid eNO measurement during natural tidal breathing without requiring patient cooperation for forced exhalation. This approach facilitates the diagnosis of airway inflammation, early detection, and precise treatment, particularly for patients unable to perform traditional fractional exhaled nitric oxide (FeNO&lt;sub&gt;50&lt;/sub&gt;, a constant flow rate of 50 mL/s sustained for 10 seconds) testing, such as children, the elderly, and severely ill patients. However, unlike FeNO&lt;sub&gt;50&lt;/sub&gt;, TBNO lacks established reference values for adult asthma patients globally.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This prospective validation study enrolled adult asthma patients and healthy volunteers from two tertiary hospitals in China. Participants were categorized into two subgroups: healthy volunteers and asthmatics. All the eNOglow (chemiluminescence-based) analyzer and conventional electrochemical-based analyzers (ECH) were utilized to measure eNO levels in all subjects at the pulmonary function clinic. Each participant underwent three FeNO&lt;sub&gt;50&lt;/sub&gt; tests using both analyzers and three TBNO tests using the eNOglow device. The clinical performance and stability of eNOglow were evaluated in both healthy volunteers and asthmatic patients, comparing its results with conventional FeNO&lt;sub&gt;50&lt;/sub&gt; parameters obtained from widely used clinical eNO analyzers. Additionally, a reference value range for TBNO was established based on the collected data. The receiver-operating characteristic (ROC) curve was applied, and the best cut-off value was selected based on the maximum Youden's index.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The study included 189 participants (131 asthma patients and 58 healthy volunteers), with a total of 1,701 tests performed. Both devices demonstrated reproducible FeNO&lt;sub&gt;50&lt;/sub&gt; values across all subjects. The coefficient of variation (CV) for eNOglow was significantly lower than that of ECH, with overall CVs of 2.82% (1.81, 4.96) for eNOglow and 11.81% (6.31, 19.95) for ECH (P &lt; 0.0001). FeNO&lt;sub&gt;50&lt;/sub&gt; values showed a strong correlation between the two analyzers (r = 0.9093, P &lt; 0.0001). ROC curve analysis revealed that eNOglow had slightly higher sensitivity and specificity than ECH. The optimal FeNO&lt;sub&gt;50&lt;/sub&gt; cut-off value for eNOglow was 24.49 ppb, consistent with clinical guidelines and slightly higher than the 20.83 ppb cut-off for ECH. For TBNO values, the best cut-off values for predicting asthma were 7.67 ppb (corresponding to FeNO&lt;sub&gt;50&lt;/sub&gt; = 25 ppb) and 12.5 ppb (corresponding to FeNO&lt;sub&gt;50&lt;/sub&gt; = 50 ppb), based on the maximum Youden's index.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The eNOglow analyzer demonstrates reliable performance in asthmatic patients acr","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149195604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"It's all in the timing: The role of Nitric Oxide in measuring diffusion capacity of the lung.","authors":"Rebecca M Baron, Tilo Winkler","doi":"10.1016/j.niox.2026.10.004","DOIUrl":"https://doi.org/10.1016/j.niox.2026.10.004","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative Aggregation of Cu/Zn Superoxide Dismutase Increases Sulfide Oxidase and Decreases Superoxide Dismutase Activity","authors":"Karthik Mouli ,&nbsp;Anton V. Liopo ,&nbsp;Kenneth R. Olson ,&nbsp;Luis Marcelo F. Holthauzen ,&nbsp;Paul J. Derry ,&nbsp;Thomas A. Kent","doi":"10.1016/j.niox.2026.05.040","DOIUrl":"10.1016/j.niox.2026.05.040","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Page S18"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148342867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 8th World Congress on Hydrogen Sulfide in Biology and Medicine, Fribourg, Switzerland, July 1-3, 2026","authors":"","doi":"10.1016/j.niox.2026.06.004","DOIUrl":"10.1016/j.niox.2026.06.004","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Pages S3-S5"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of H2S in isoproterenol-induced cardiac hypertrophy","authors":"Alexandra Gyöngyösi,&nbsp;Simon Eskeif,&nbsp;Richard Kajtár,&nbsp;Ferenc Fenyvesi,&nbsp;Adina Fésűs,&nbsp;Pál Herczeg,&nbsp;Anikó Borbás,&nbsp;Ilona Bakai-Bereczki,&nbsp;István Lekli","doi":"10.1016/j.niox.2026.05.025","DOIUrl":"10.1016/j.niox.2026.05.025","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Page S13"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148565200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H2S–SYNTHESIZING ENZYMES may play a role IN Breast CANCER CHEMORESISTANCE","authors":"Susana Parreiras,&nbsp;Cindy Mendes,&nbsp;Isabel Lemos,&nbsp;Diana Lousa,&nbsp;João B. Vicente,&nbsp;Jacinta Serpa","doi":"10.1016/j.niox.2026.05.053","DOIUrl":"10.1016/j.niox.2026.05.053","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Page S22"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148565864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STRUCTURAL AND MECHANISTIC INSIGHTS OF HYDROGEN SULFIDE PRODUCTION DURING AGGREGATION BY CYSTATHIONINE BETA-SYNTHASE","authors":"Elias Nieto-Zaragoza,&nbsp;Emilio Espinoza-Simon,&nbsp;Artem Stetsenko,&nbsp;Nuria Sanchez-Puig,&nbsp;Albert Guskov,&nbsp;Francisco Torres-Quiroz","doi":"10.1016/j.niox.2026.05.045","DOIUrl":"10.1016/j.niox.2026.05.045","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Pages S19-S20"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148565996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H2S Drives Slow-Fiber Remodeling and Restores PGC-1α Signaling in Duchenne Muscular Dystrophy","authors":"Valentina Vellecco,&nbsp;Veronica Casale,&nbsp;Martina Smimmo,&nbsp;Giuseppe Cirino,&nbsp;Milos Filipovic,&nbsp;Mariarosaria Bucci","doi":"10.1016/j.niox.2026.05.079","DOIUrl":"10.1016/j.niox.2026.05.079","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Page S30"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148572196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRANSLATIONAL AND REGULATORY CHALLENGES OF THERAPEUTIC GASES IN PHARMACEUTICAL DEVELOPMENT","authors":"Shams Al-Ani,&nbsp;Hao Xu,&nbsp;Anthony Lockett,&nbsp;Alex Dyson","doi":"10.1016/j.niox.2026.05.003","DOIUrl":"10.1016/j.niox.2026.05.003","url":null,"abstract":"","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"163 ","pages":"Page S6"},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148572619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}