Nitric oxide : biology and chemistry最新文献

The Nitric oxide Society and the Nitric Oxide Journal: Returning to Our Foundation to Shape the Future of Redox Biology. 一氧化氮学会和一氧化氮杂志：回到我们的基础，塑造氧化还原生物学的未来。

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-05-21 DOI: 10.1016/j.niox.2025.05.005

Miriam M Cortese-Krott, Lorenzo Berra, Nathan S Bryan, Mattias Carlström, Sharon Glynn, Adrian Hobbs, Katrina Miranda, Hozumi Motohashi, Motohiro Nishida, Ciara E O'Neil, Jesus Tejero

引用次数: 0

The association between serum and urinary nitric oxide metabolites and fatty liver index: a population-based study. 血清和尿一氧化氮代谢物与脂肪肝指数之间的关系：一项基于人群的研究。

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-05-20 DOI: 10.1016/j.niox.2025.05.004

Zahra Bahadoran, Fereidoun Azizi, Asghar Ghasemi

{"title":"The association between serum and urinary nitric oxide metabolites and fatty liver index: a population-based study.","authors":"Zahra Bahadoran, Fereidoun Azizi, Asghar Ghasemi","doi":"10.1016/j.niox.2025.05.004","DOIUrl":"https://doi.org/10.1016/j.niox.2025.05.004","url":null,"abstract":"<p><strong>Background and aim: </strong>We investigated the association between fasting serum and urinary nitric oxide metabolite (NOx) levels and fatty liver index (FLI), a non-invasive surrogate of non-alcoholic fatty liver disease (NAFLD) and liver steatosis.</p><p><strong>Method: </strong>This cross-sectional study included 598 adults (aged≥18 years, 48.6% men) who participated in the Tehran Lipid and Glucose Study (2015-2017). Serum and urine NOx concentrations were quantified using a spectrophotometric method following the Griess reaction. FLI values were calculated using γ-glutamyl transferase, triglycerides, body mass index, and waist circumference. The associations between urinary and serum NOx-to-creatinine (Cr) ratio [either as a categorical variable, i.e., tertiles, or as a continuous variable, i.e., per 1 SD) with NAFLD (i.e., FLI≥60) were assessed using multivariable-adjusted binary logistic regression.</p><p><strong>Results: </strong>The study participants' mean (SD) age was 42.5±14.6 y. The mean (SD) of serum and urinary NOx was 37.5±16.7 and 1310±751 μmol/L, respectively. The mean (SD) of FLI was 43.3±30.2, and the prevalence of NAFLD was 32.4%. Serum NOx-to-Cr ratio was not associated with the chance of having NAFLD (OR=1.66, 95% CI=0.98-2.82; P value=0.058). Higher urinary NOx-to-Cr ratio was significantly associated with a reduced probability of NAFLD (OR=0.61, 95% CI=0.38-0.95, and OR=0.54, 95% CI=0.34-0.87, in the second and third tertiles).</p><p><strong>Conclusion: </strong>Higher dietary nitrate (NO<sub>3</sub>) intake, indicated by increased urinary NOx-to-Cr ratio, is associated with a reduced probability of NAFLD, highlighting the potential role of dietary NO<sub>3</sub> in liver health.</p>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute effects of l-arginine intake on heart rate variability after a submaximal exercise test in healthy men: randomized clinical trial 健康男性次最大运动试验后左旋精氨酸摄入对心率变异性的急性影响：随机临床试验

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-05-17 DOI: 10.1016/j.niox.2025.05.003

Andrey Alves Porto , Luana Almeida Gonzaga , Rayana Loch Gomes , Bruno M. Candeloro , Rodrigo Daminello Raimundo , Luiz Carlos Marques Vanderlei , Vitor Engrácia Valenti

{"title":"Acute effects of l-arginine intake on heart rate variability after a submaximal exercise test in healthy men: randomized clinical trial","authors":"Andrey Alves Porto , Luana Almeida Gonzaga , Rayana Loch Gomes , Bruno M. Candeloro , Rodrigo Daminello Raimundo , Luiz Carlos Marques Vanderlei , Vitor Engrácia Valenti","doi":"10.1016/j.niox.2025.05.003","DOIUrl":"10.1016/j.niox.2025.05.003","url":null,"abstract":"<div><div><span>l</span>-arginine (L-ARG) is a semi-essential amino acid and a precursor for nitric oxide (NO) synthesis via endothelial nitric oxide synthase (eNOS). While NO has been implicated in autonomic modulation and vascular function, the effects of acute L-ARG intake on heart rate variability (HRV) after exercise remain unclear. This study aimed to assess the acute effects of L-ARG supplementation on HRV and cardiovascular recovery following submaximal aerobic exercise in healthy men. In a triple-blind, randomized, placebo-controlled, crossover trial, 37 physically active males (18–30 years) underwent two treadmill exercise protocols: one following ingestion of 3 g of L-ARG and the other with placebo. HRV and cardiovascular parameters (heart rate, systolic and diastolic blood pressure) were measured at baseline and across 20 min of post-exercise recovery. Time- and frequency-domain HRV indices were analyzed using validated algorithms. Two-way repeated measures ANOVA and post-hoc tests were applied (<em>p</em> < 0.05). Significant time effects were observed for HRV and cardiovascular variables across both protocols (<em>p</em> < 0.001), indicating physiological recovery. However, no statistically significant differences were found between the L-ARG and placebo conditions for any HRV or hemodynamic outcome. A trend toward faster vagal reactivation (rMSSD) was observed with L-ARG, but effect sizes were small and not clinically relevant. Acute supplementation with 3 g of <span>l</span>-arginine did not significantly influence HRV or cardiovascular recovery following submaximal aerobic exercise in healthy young men. These findings suggest limited autonomic effects of L-ARG in populations with high baseline HRV.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 68-73"},"PeriodicalIF":3.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide based therapies for peripheral artery disease: Evidence and opportunities. 基于一氧化氮的外周动脉疾病治疗：证据和机会。

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-05-16 DOI: 10.1016/j.niox.2025.05.002

Mary M McDermott, Daniel B Kim-Shapiro, Jason D Allen

引用次数: 0

Generation of high-concentration medical-grade nitric oxide via air-sourced pulsed arc discharge 空气源脉冲电弧放电产生高浓度医用级一氧化氮

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-05-12 DOI: 10.1016/j.niox.2025.05.001

Weiming Ni , Haixiao Wu , Guangqing Liu , Jun Li , Yegang Lu

{"title":"Generation of high-concentration medical-grade nitric oxide via air-sourced pulsed arc discharge","authors":"Weiming Ni , Haixiao Wu , Guangqing Liu , Jun Li , Yegang Lu","doi":"10.1016/j.niox.2025.05.001","DOIUrl":"10.1016/j.niox.2025.05.001","url":null,"abstract":"<div><div>Inhaled nitric oxide (<em>NO</em>) is a selective pulmonary vasodilator that has been shown to be effective in treating pulmonary hypertension in both adults and children with respiratory and heart failure. The most common method for delivering <em>NO</em> in clinical practice relies on compressed high-concentration <em>NO</em> gas stored in cylinders. This <em>NO</em> gas is typically synthesized through chemical methods and stored in high-concentration form within gas cylinders, making <em>NO</em> therapy equipment bulky and expensive. Moreover, chemical synthesis requires specific materials, and the production and storage of <em>NO</em> are two separate steps, both of which are complex and costly. These factors limit the widespread adoption of <em>NO</em> therapy. To address the issues associated with high-concentration <em>NO</em> cylinder therapy, this study explores a method for generating <em>NO</em> gas through the ionization of air via pulsed arc discharge, and further investigates strategies to increase its concentration. A portable <em>NO</em> generator is designed to produce high-concentration <em>NO</em> gas on-demand, providing a potential alternative to cylinder storage. The results show that the four-electrode <em>NO</em> generator can quickly and safely produce 545.2 ± 10 ppm of <em>NO</em> and reduce the co-production of other gases. The pulsed arc <em>NO</em> generator designed in this study generates sufficiently high concentrations of <em>NO</em> gas to replace cylinder storage with lightweight, portable, and capable of immediate on-demand generation. The generator produces <em>NO</em> gas from ambient air, thus significantly reducing the cost of inhaled <em>NO</em> therapy and paving the way for wider application of this therapy.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 55-65"},"PeriodicalIF":3.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New directions for Nitric Oxide – Focused, rapid, and forward-looking 一氧化氮的新方向——聚焦、快速和前瞻性。

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-04-22 DOI: 10.1016/j.niox.2025.04.005

Miriam M. Cortese-Krott , Lorenzo Berra , Adrian Hobbs , Katrina M. Miranda , Hozumi Motohashi , Jesus Tejero

引用次数: 0

Hydrogen sulfide detection: Recent advancement and future perspectives towards fluorescence as a versatile Biophysical method 硫化氢检测：荧光作为一种多功能生物物理方法的最新进展和未来展望

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-04-22 DOI: 10.1016/j.niox.2025.04.004

Vivek Pandey , Tejasvi Pandey

{"title":"Hydrogen sulfide detection: Recent advancement and future perspectives towards fluorescence as a versatile Biophysical method","authors":"Vivek Pandey , Tejasvi Pandey","doi":"10.1016/j.niox.2025.04.004","DOIUrl":"10.1016/j.niox.2025.04.004","url":null,"abstract":"<div><div>Hydrogen Sulfide (H<sub>2</sub>S) is an essential gaseous signaling molecule involved in various physiological processes, including vasodilation, neurotransmission, and anti-inflammatory responses. Accurate detection and quantification of H<sub>2</sub>S in biological systems are crucial for elucidating its physiological and pathological roles. Fluorescent probes have emerged as indispensable tools for H<sub>2</sub>S detection, offering high sensitivity, specificity, and the ability for real-time and non-invasive monitoring. This review discusses recent advances in the design and development of fluorescent probes for H<sub>2</sub>S detection, focusing on their mechanisms, properties, and applications. We explore the different strategies employed in probe design, including reduction-based mechanisms, nucleophilic addition reactions, and cleavage of sulfide bonds. Innovations such as ratiometric probes, two-photon fluorescent probes, and multi-functional probes have significantly enhanced the capabilities of H<sub>2</sub>S detection. These advancements have facilitated cellular and subcellular imaging, real-time monitoring in live organisms, and the investigation of H<sub>2</sub>S-related pathologies. Despite these progresses, challenges remain, including improving probe selectivity, stability, and biocompatibility, as well as developing methods for accurate quantification in complex biological matrices. Future research directions include designing probes with higher selectivity and sensitivity, integrating advanced computational modeling, and combining fluorescent probes with mass spectrometry for precise quantification. The continued development of sophisticated fluorescent probes will expand our understanding of H<sub>2</sub>S biology, offering new insights into its physiological and pathological roles and paving the way for novel therapeutic strategies.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 34-45"},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide detection by electrochemistry selective probe: calibration in the study environment is mandatory 电化学选择性探针检测一氧化氮：在研究环境中校准是强制性的

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-04-19 DOI: 10.1016/j.niox.2025.04.002

Roberta Albino Dos Reis , Ariane Boudier , Flavian Piquard , Joana C. Piereti , Amedea B. Seabra , Igor Clarot

{"title":"Nitric oxide detection by electrochemistry selective probe: calibration in the study environment is mandatory","authors":"Roberta Albino Dos Reis , Ariane Boudier , Flavian Piquard , Joana C. Piereti , Amedea B. Seabra , Igor Clarot","doi":"10.1016/j.niox.2025.04.002","DOIUrl":"10.1016/j.niox.2025.04.002","url":null,"abstract":"<div><div>Nitric oxide (NO) plays a crucial role in various physiological processes, making its detection and controlled release significant for both therapeutic and environmental contexts. Electrochemical sensors are widely used for NO detection due to their high sensitivity and real-time monitoring capabilities. However, challenges such as interference from other gasotransmitters, sensor degradation, and calibration difficulties—especially in complex biological matrices—hinder accurate NO measurement. This review discusses recent advancements in electrochemical NO detection, with a focus on the impact of complex biological matrices, calibration strategies, and sensor designs. The release of NO from nanoparticles, such as S-nitrosoglutathione (GSNO)-encapsulating chitosan nanoparticles, is used as a case study for improving NO detection accuracy. Future innovations in sensor technology and nanoparticle design are expected to expand the applicability of NO detection in personalized medicine and environmental monitoring.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 46-54"},"PeriodicalIF":3.2,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic interaction of sodium nitroprusside and Serratia marcescens in mitigation of nematode stress in tomato 硝普钠和肉质沙雷氏菌在缓解番茄线虫胁迫方面的协同作用

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-04-19 DOI: 10.1016/j.niox.2025.04.003

Deepak Kumar , Rajesh Kumari Manhas , Puja Ohri

{"title":"Synergistic interaction of sodium nitroprusside and Serratia marcescens in mitigation of nematode stress in tomato","authors":"Deepak Kumar , Rajesh Kumari Manhas , Puja Ohri","doi":"10.1016/j.niox.2025.04.003","DOIUrl":"10.1016/j.niox.2025.04.003","url":null,"abstract":"<div><div>Plant growth and development are negatively impacted by root-knot nematodes (RKNs), which in turn affects plant production. Chemical nematicides are one of the effective strategies for managing RKNs. But, high concentration of these chemicals is toxic to plants, environment and humans. Therefore, an <em>in-vivo</em> study was conducted to unravel the synergistic interplay sodium nitroprusside (SNP: nitric oxide donor) and, <em>Serratia marcescens</em> in <em>M. incognita</em>-stressed tomato plants. Results revealed that treatment with SNP and bacterial culture cells reduced gall formation and improved morphology. It also reduced nematode-induced oxidative stress in <em>M. incognita-</em>infested tomato plants as compared to untreated plants. Increased photosynthetic parameters including photosynthetic pigments and gas-exchange parameters was also observed in treated plants. Additionally, treated plants exhibited increased antioxidant defense system in terms of upregulated activities of enzymatic antioxidants (Ascorbate peroxidase, guaiacol peroxidase, polyphenol oxidase, catalase, glutathione-S-transferase and superoxide dismutase). Content of non-enzymatic antioxidants (Glutathione, ascorbic acid and tocopherol) was also enhanced in treated plants as compared to untreated nematode-infected plants. Further, treatment with SNP and <em>S. marcescens</em> increased secondary metabolites (total phenol, flavonoid and anthocyanin) and proline content. Reduction in nematode-induced nuclear and membrane damage was also observed in SNP and bacterial culture cells treated tomato plants. The integrative application of SNP and <em>S. marcescens</em> exhibited synergism and overpowered their individual application in reducing the negative effects of nematode stress. The findings of the current investigation suggest the integrative use of SNP and bacteria is more beneficial in alleviating nematode stress in plants.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 1-18"},"PeriodicalIF":3.2,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is there a link between the abundance of nitrate-reducing bacteria and arterial hypertension? A systematic review 减少硝酸盐的细菌和动脉高血压之间是否存在联系？系统回顾

IF 3.2 2区生物学

Nitric oxide : biology and chemistry Pub Date : 2025-04-11 DOI: 10.1016/j.niox.2025.04.001

Esthela M. Puel , Lillian F. Taruhn , Nailê Damé-Teixeira , Cristine M. Stefani , Renata M. Lataro

{"title":"Is there a link between the abundance of nitrate-reducing bacteria and arterial hypertension? A systematic review","authors":"Esthela M. Puel , Lillian F. Taruhn , Nailê Damé-Teixeira , Cristine M. Stefani , Renata M. Lataro","doi":"10.1016/j.niox.2025.04.001","DOIUrl":"10.1016/j.niox.2025.04.001","url":null,"abstract":"<div><h3>Context</h3><div>Nitric oxide is a vasodilator molecule that acts on blood pressure (BP) control, and its production can occur through the reduction of nitrates by oral or intestinal nitrate-reducing bacteria. However, the relationship between nitrate-reducing bacteria and arterial hypertension (HTN) remains under debate.</div></div><div><h3>Objective</h3><div>Systematically review if there is an association between the abundance of oral and intestinal nitrate-reducing bacteria and the occurrence of HTN in humans.</div></div><div><h3>Databases and eligibility criteria</h3><div>MEDLINE, Scopus, Cochrane Library, EMBASE, LILACS, Web of Science, Livivo, ProQuest Dissertations, and Google Scholar were searched for eligible articles until February 10th, 2024. Studies were included if they: (1) were observational studies or clinical trials; (2) included adults (≥18 years old) with HTN (systolic BP ≥ 130 mmHg and/or diastolic BP > 80 mmHg and/or use of BP lowering medication); (3) compared (or not) to no-HTN adults; and (4) used next-generation sequencing microbiome analysis to identify bacterial taxa in the oral and/or gut nitrate-reducing bacteria.</div></div><div><h3>Results</h3><div>The search identified 9365 articles, and 28 were included in the study after applying the inclusion and exclusion criteria; 23 articles assessed the gut microbiota, 4 assessed the oral microbiota, and 1 assessed both. Depletion of nitrate-reducing bacteria was not consistently shown in the studies. The included studies reported reduction, increase, and no change in the nitrate-reducing bacteria genera or species in oral or gut microbiota.</div></div><div><h3>Conclusion</h3><div>We found no association between the abundance of oral and gut nitrate-reducing bacteria and the occurrence of HTN in humans.</div></div><div><h3>Registration</h3><div>PROSPERO identification number CRD42022315891.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"157 ","pages":"Pages 19-33"},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0