Nitric oxide : biology and chemistry最新文献

{"title":"From methionine to sulfide: exploring the diagnostic and therapeutic potential of sulfur-containing biomolecules in hypertension.","authors":"Anton Misak, Marian Grman, Karol Ondrias, Lenka Tomasova","doi":"10.1016/j.niox.2025.03.006","DOIUrl":"https://doi.org/10.1016/j.niox.2025.03.006","url":null,"abstract":"<p><p>Sulfur-containing amino acids are involved in the regulation of vascular activity and blood pressure. Clinically, a positive correlation was found between serum homocysteine levels and blood pressure. On the other hand, methionine and cysteine levels were reduced in hypertensive patients. Recently, the redox state of sulfur-containing amino acids has emerged as potential diagnostic marker of cardiovascular health. Metabolomic studies have revealed a shift in thiol/disulfide ratio toward oxidized forms and overproduction of thiyl radicals in hypertensive patients. Although accumulating evidence confirms that sulfur-containing amino acids are essential for the maintaining redox homeostasis and blood pressure control, their hypotensive and antioxidant properties have been primarily demonstrated in animal studies. While several groups are developing new targeted and triggered sulfur-based donors, standardized pharmacological interventions for hypertensive patients are largely absent and pose a challenge for future research. In this review, we summarize recent studies that investigate the role of sulfur-containing amino acids and their redox-active metabolites, including glutathione and sulfide, in blood pressure control and the development of systemic hypertension.</p>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appraising diverse metrics of nitric oxide in salt stress tolerance of high yielding wheat genotypes","authors":"Sayeda Khatoon,&nbsp;Sarika Kumari,&nbsp;Muskan Gandhi,&nbsp;Kritika Nagarwal,&nbsp;Rudra Narayan Sahoo,&nbsp;Noushina Iqbal,&nbsp;M. Iqbal R. Khan","doi":"10.1016/j.niox.2025.03.005","DOIUrl":"10.1016/j.niox.2025.03.005","url":null,"abstract":"<div><div>Staple crop vulnerability has been escalating with significant approach due to climatic variations leading to persistent salt accumulation as inimical environmental stressors, and thus endangering food security. To address this global concern, there is a need to elucidate the growth, physiological and yield responses, entailing plant salt tolerance modifications. Recent years have been advocated with studies focusing on the integration of nitric oxide (NO), however there is a need of critical decipherment on NO synthesis is regulated under salt stress conditions. With this focus, the present investigation has assessed the salt-mediated differential impacts on the plant growth, root architecture, photosynthetic pigment, carbon metabolites (carbohydrate and starch), and stomatal frequency, leading to restrained plant metabolisms in the 49 wheat genotypes. Further, the accumulation of secondary metabolites (flavonoids and phenols) was found concomitant with the improved NO biosynthesis in salt-stressed tolerant wheat genotype. To validate the involvement of endogenous NO as salt stress tolerance criterion, use of NO scavenger (cPTIO) suggests the involvement of NO in enhancing salt tolerance and stress defense metabolites mainly lignin biosynthesis, and cellulose to attain plant stress tolerance. These underlying interactions could pave the way to convey wheat tolerance for the future breeding programs.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 82-93"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of beetroot juice in patients with peripheral artery disease: A Narrative review","authors":"Hao Wang ,&nbsp;Mingming Chen ,&nbsp;Yang Li ,&nbsp;Wenjun Cui ,&nbsp;Qian An ,&nbsp;Xiangyang Yin ,&nbsp;Bing Wang","doi":"10.1016/j.niox.2025.03.003","DOIUrl":"10.1016/j.niox.2025.03.003","url":null,"abstract":"<div><div>Peripheral artery disease (PAD) is a circulatory disorder caused by atherosclerosis, leading to the narrowing or blockage of peripheral arteries, often affecting the arteries in the lower limbs. This condition can result in intermittent claudication and severe limb ischemia, significantly reducing patients' quality of life. In recent years, increasing evidence suggests that dietary interventions play a crucial role in the prevention and management of PAD, offering a safe and non-invasive treatment option. Beetroot, a natural root vegetable, demonstrates significant health benefits through its various bioactive compounds. It is rich in nitrate and betaine, which are metabolized in the body via the nitrate-nitrite- nitric oxide (NO) pathway, increasing the bioavailability of NO. NO is an important vasodilator that can improve blood flow and lower blood pressure. Additionally, the active compounds in beetroot may further enhance its health effects by altering the activity of the oral microbiome. This review explores the potential therapeutic effects of beetroot juice (BRJ) in the management of PAD. The findings indicate that BRJ can improve exercise performance, lower blood pressure, improve endothelial function, enhance skeletal muscle microvascular function and central autonomic nervous system function. Based on these findings, beetroot and its rich bioactive compounds hold promise as a novel supportive therapy for improving PAD.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 57-66"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative nitric oxide-releasing nanomaterials: Current progress, trends, challenges, and perspectives in cardiovascular therapies","authors":"Renan S. Nunes,&nbsp;Kelli C. Freitas Mariano,&nbsp;Joana C. Pieretti,&nbsp;Roberta A. dos Reis,&nbsp;Amedea B. Seabra","doi":"10.1016/j.niox.2025.03.004","DOIUrl":"10.1016/j.niox.2025.03.004","url":null,"abstract":"<div><div>Cardiovascular diseases remain the leading cause of death worldwide, imposing a substantial impact on healthcare systems due to high morbidity, mortality, and associated economic costs. Nitric oxide (NO), a key signaling molecule in the cardiovascular system, plays a critical role in regulating vascular homeostasis, angiogenesis, and inflammation. Despite its therapeutic potential, direct NO delivery in the cardiovascular system is limited by its reactivity, short half-life, and poor bioavailability. The development of NO-releasing nanomaterials addresses these challenges by enabling controlled, targeted, and sustained NO delivery, mitigating systemic toxicity and improving therapeutic outcomes. This review provides a comprehensive overview of recent advancements in the design, functionalization, and application of NO-releasing nanomaterials for cardiovascular therapies. Key topics include the use of <em>in vitro</em> and <em>in vivo</em> models to evaluate efficacy in conditions such as myocardial ischemia-reperfusion injury, thrombosis, and atherosclerosis, as well as the role of stimuli-responsive systems and hybrid nanomaterials in enhancing delivery precision. Advances in nanotechnology, such as stimuli-responsive systems and hybrid functionalized nanomaterials for targeted delivery, have enhanced the precision and effectiveness of NO therapeutic effects for treating a wide spectrum of cardiovascular conditions. However, challenges like scalable production, biocompatibility, and integration with existing therapies remain. Future research should focus on interdisciplinary approaches to optimize these materials for clinical translation, ensuring accessibility and addressing the global problem of cardiovascular diseases.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 67-81"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring medical gas therapy in hemorrhagic stroke treatment: A narrative review","authors":"Liang Cao ,&nbsp;Chen Chen ,&nbsp;Wenjun Pi ,&nbsp;Yi Zhang ,&nbsp;Sara Xue ,&nbsp;Voon Wee Yong ,&nbsp;Mengzhou Xue","doi":"10.1016/j.niox.2025.03.002","DOIUrl":"10.1016/j.niox.2025.03.002","url":null,"abstract":"<div><div>Hemorrhagic stroke (HS) is a neurological disorder caused by the rupture of cerebral blood vessels, resulting in blood seeping into the brain parenchyma and causing varying degrees of neurological impairment, including intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SAH). Current treatment methods mainly include hematoma evacuation surgery and conservative treatment. However, these methods have limited efficacy in enhancing neurological function and prognosis. The current challenge in treating HS lies in inhibiting the occurrence and progression of secondary brain damage after bleeding, which is a key factor affecting the prognosis of HS patients. Studies have shown that medical gas therapy is gaining more attention and has demonstrated various levels of neuroprotective effects on central nervous system disorders, such as hyperbaric oxygen, hydrogen sulfide, nitric oxide, carbon monoxide, and other inhalable gas molecules. These medical gas molecules primarily improve brain tissue damage and neurological dysfunction by regulating inflammation, oxidative stress, apoptosis, and other processes. However, many of these medical gasses also possess neurotoxic properties. Therefore, the use of medical gases in HS deserves further exploration and research. In this review, we will elucidate the therapeutic effects and study the advances in medical gas molecules in HS.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 94-106"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing nitric oxide availability via ingestion of nitrate-rich beetroot juice improves vascular responsiveness in individuals with Alzheimer's Disease","authors":"Anna Pedrinolla ,&nbsp;Gianluigi Dorelli ,&nbsp;Simone Porcelli ,&nbsp;Mia Burleigh ,&nbsp;Martina Mendo ,&nbsp;Camilla Martignon ,&nbsp;Cristina Fonte ,&nbsp;Luca Giuseppe Dalle Carbonare ,&nbsp;Chris Easton ,&nbsp;Ettore Muti ,&nbsp;Federico Schena ,&nbsp;Massimo Venturelli","doi":"10.1016/j.niox.2025.03.001","DOIUrl":"10.1016/j.niox.2025.03.001","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Poor vascular function and reduced nitric oxide (NO)-bioavailability have been recognized to be involved in aging and Alzheimer's Disease (AD). A non-pharmacological treatment that is gaining clinical interest in the context of vascular function is dietary inorganic nitrate (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) supplementation which increases NO-bioavailability through the &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; -nitrite (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) - NO pathway. This treatment has been demonstrated to improve vascular function in several clinical populations, but no study has investigated the effects in individuals with AD. Therefore, changes in plasma &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and vascular responsiveness (hyperemic response to single-passive leg movement (ΔPLM)) were measured in individuals with AD (n = 10, 76 ± 9 years), healthy elderly (OLD, n = 10, 75 ± 6 years), and young individuals (YN, n = 10, 25 ± 4 years) before (T0) and hourly for 4 h (T1, T2, T3, and T4) after ingestion of either &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;-rich beetroot juice (BR) or a placebo (PLA). No changes in &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, nor ΔPLM were detected in any group following PLA intake. Plasma &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; increased significantly in all three groups at T1 (p &lt; 0.001) and remained elevated for the rest of the trial. The same trend was found in ΔPLM, which significantly increased in all three groups over the time (p &lt; 0.001). However, AD exhibited significantly lower ΔPLM values at any time point compared to YN (p &lt; 0.001) and OLD (p &lt; 0.001). These data suggest that AD-individuals included in this study were able to reduce &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; to &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;N&lt;/mi&gt;&lt;mi&gt;O&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;/msubsup&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and to increase NO-mediated vascular responsiveness as non-AD-individuals. Other mechanisms, beyond NO-bioavailability, may be involved in vascular dysfunction in patients with AD. This resea","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 50-56"},"PeriodicalIF":3.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular actions of Ang 1–7 and Ang 1–8 through EDRFs and EDHFs in non-diabetes and diabetes mellitus","authors":"Nazar M. Shareef Mahmood,&nbsp;Almas M.R. Mahmud,&nbsp;Ismail M. Maulood","doi":"10.1016/j.niox.2025.02.003","DOIUrl":"10.1016/j.niox.2025.02.003","url":null,"abstract":"<div><div>The renin-angiotensin system (RAS) plays a pivotal role in regulating vascular homeostasis, while angiotensin 1-8 (Ang 1–8) traditionally dominates as a vasoconstrictor factor. However, the discovery of angiotensin 1-7 (Ang 1–7) and Ang 1–8 has revealed counter-regulatory mechanisms mediated through endothelial-derived relaxing factors (EDRFs) and endothelial-derived hyperpolarizing factors (EDHFs). This review delves into the vascular actions of Ang 1–7 and Ang 1–8 in both non-diabetes mellitus (non-DM) and diabetes mellitus (DM) conditions, highlighting their effects on vascular endothelial cell (VECs) function as well. In a non-DM vasculature context, Ang 1–8 demonstrate dual effect including vasoconstriction and vasodilation, respectively. Additionally, Ang 1–7 induces vasodilation upon nitric oxide (NO) production as a prominent EDRFs in distinct mechanisms. Further research elucidating the precise mechanisms underlying the vascular actions of Ang 1–7 and Ang 1–8 in DM will facilitate the development of tailored therapeutic interventions aimed at preserving vascular health and preventing cardiovascular complications.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 9-26"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen sulfide mechanism of action in plants; from interaction with regulatory molecules to persulfidation of proteins","authors":"Shirin Mohammadbagherlou ,&nbsp;Elaheh Samari ,&nbsp;Mostafa Sagharyan ,&nbsp;Meisam Zargar ,&nbsp;Moxian Chen ,&nbsp;Abazar Ghorbani","doi":"10.1016/j.niox.2025.02.001","DOIUrl":"10.1016/j.niox.2025.02.001","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S), previously known as a toxic gas, is currently considered one of the most important gaseous transmitters in plants. This novel signaling molecule has been determined to play notable roles in plant growth, development, and maturation. In addition, pharmacological and genetic evidence indicated that this regulatory molecule effectively ameliorates various plant stress conditions. H₂S is involved in these processes by changing gene expression, enzyme activities, and metabolite concentrations. During its regulatory function, H₂S interacts with other signaling pathways such as hydrogen peroxide (H₂O₂), nitric oxide (NO), Ca²⁺, carbon monoxide (CO), phosphatidic acid (PA), phytohormones, etc. The H₂S mechanism of action may depend on the persulfidation post-translational modification (PTM), which attacks the cysteine (Cys) residues on the target proteins and changes their structure and activities. This review summarized H₂S biosynthesis pathways, its role in sulfide state, and its donors in plant biology. We also discuss recent progress in the research on the interactions of H₂S with other signaling molecules, as well as the role of persulfidation in modulating various plant reactions.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 27-41"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time nitric oxide detection in cytokine stimulated cancer cells and macrophages","authors":"Jennifer Daw ,&nbsp;Su Chung ,&nbsp;Cheng-Yu Chen ,&nbsp;Ronald L. Heimark ,&nbsp;William R. Montfort","doi":"10.1016/j.niox.2025.02.004","DOIUrl":"10.1016/j.niox.2025.02.004","url":null,"abstract":"<div><div>Inflammation is increasingly linked to disease progression, particularly in cancer, where elevated levels of inducible nitric oxide synthase (iNOS or NOS2), driven by tumor inflammation, is correlated with aggressive tumors and poor outcomes. Measuring nitric oxide levels in tumor cells is hampered by the reactive nature of the molecule and generally inferred through indirect measurement of reaction products such as nitrate and nitrite. Here, we adapt the oxyhemoglobin detection method to tissue culture and examine nitric oxide production in tumor cells in response to inflammatory cytokines. Our assay provides real-time nitric oxide measurement, is highly sensitive, linear for at least an hour, inexpensive, and easy to implement. We show that triple negative breast and colorectal cancer cells respond to interferon gamma (IFNγ), interleukin 1-β (IL1-β) and tumor necrosis factor α (TNFα) to generate surprisingly high levels of NOS2 protein and nitric oxide, as high as seen in activated macrophages for fighting infection. NO detection levels reach 1.3 pmol NO/min/μg total cellular protein. The assay is readily adapted to assessing IC50 values for NOS2 inhibition, inhibition rates, and inhibition persistence. Using triple negative breast cancer cell line 4T1, a syngeneic murine tumor model, we estimate an IC₅₀ = 3.4 μM for NOS2-specific inhibitor 1400W, which displays a low nanomolar binding constant to isolated protein. Inhibition is rapid (&lt;10 min) and persists for at least an hour. These results highlight the importance of nitric oxide production in the tumor and provide a means for developing new therapeutic strategies.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 42-49"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The new perspective of gasotransmitters in cancer metastasis","authors":"Yu-Bo Shi ,&nbsp;Lin Cheng ,&nbsp;Yue Lyu ,&nbsp;Ze-Jing Shi","doi":"10.1016/j.niox.2025.02.002","DOIUrl":"10.1016/j.niox.2025.02.002","url":null,"abstract":"<div><div>Cancer metastasis is the leading cause of death in cancer patients, which renders heavy burdens to family and society. Cancer metastasis is a complicated process in which a large variety of biological molecules, cells and signaling pathways are involved. Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H₂S) are common air pollutants which are harmful to human bodies and environments. However, recent studies show that these gases, which are collectively termed gasotransmitters, play significant roles in physiological homeostasis and pathogenesis including immunological responses, neuronal regulations, respiratory as well as cardiovascular diseases, metabolic disorders and cancers. These gases are abnormally expressed in cancer cells or tissues, along with the gas-producing enzymes. They have been demonstrated to participate in cancer metastasis intensively by modulating diverse signaling axes. This review introduces the nature of gasotransmitters, summaries novel research progression in gasotransmitters-induced cancer metastasis and elucidates multifaceted mechanisms how the process is modulated, with an effort to bring new therapeutic targets for cancer management in the future.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"156 ","pages":"Pages 1-8"},"PeriodicalIF":3.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}