Nitric oxide : biology and chemistry最新文献

{"title":"SIRT3 sulfhydrylation by hydrogen sulphide: A novel approach to prevent ferroptosis and atrial fibrosis","authors":"Zi-Zhu Zhang ,&nbsp;Xiu-Heng Wang ,&nbsp;Zhi-Hong Ning ,&nbsp;Yun Ou ,&nbsp;Jia-Yan Yang ,&nbsp;Hui-Fang Tang ,&nbsp;Zhi-Sheng Jiang ,&nbsp;Heng-Jing Hu","doi":"10.1016/j.niox.2025.06.006","DOIUrl":"10.1016/j.niox.2025.06.006","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogen sulfide (H₂S) has been shown to counteract ferroptosis and atrial fibrosis, yet the underlying mechanisms remain incompletely understood. This study aims to investigate how H₂S regulates ferroptosis to alleviate atrial fibrosis.</div></div><div><h3>Methods</h3><div>In vivo and in vitro models of atrial fibrosis were established using Angiotensin II (Ang-II) to modulate the expression of SIRT3, β-catenin, and ferroptosis markers. Western blotting was employed to analyze changes in proteins related to ferroptosis and fibrosis. Histological evaluations, including Hematoxylin and Eosin (HE), and Masson's staining were performed to assess atrial fibrosis. Cardiac ultrasound was used to assess left atrial function in vivo. In vitro, reactive oxygen species (ROS) levels and iron staining were used to monitor ferroptosis and oxidative stress.</div></div><div><h3>Results</h3><div>In atrial tissue from patients with AF, significant increases in ferroptosis markers and atrial fibrosis were observed. In both animal and cell models of atrial fibrosis, reduced sulfhydrylated SIRT3 and elevated β-catenin expression were associated with increased ferroptosis and fibrosis markers. Treatment with NaHS, a donor of H₂S, reversed these changes, reducing both ferroptosis and fibrosis. Importantly, inhibition of sulfhydrylated SIRT3 further upregulated β-catenin, exacerbating ferroptosis and fibrosis. However, blocking β-catenin effectively alleviated Ang–II–induced ferroptosis and fibrosis in the atrial fibrosis model.</div></div><div><h3>Conclusion</h3><div>H₂S alleviates atrial fibrosis and inhibits ferroptosis by upregulating SIRT3 sulfhydrylation and antagonizing the Wnt/β-catenin signaling pathway. These findings suggest that targeting the H₂S-SIRT3-β-catenin signaling axis may offer a promising therapeutic strategy for atrial fibrosis and associated arrhythmias.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 82-92"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic nitrate stores, astrocyte metabolism and brain health: An emerging paradigm","authors":"Mario Siervo ,&nbsp;Giuseppe Verdile ,&nbsp;Barbora Piknova","doi":"10.1016/j.niox.2025.06.005","DOIUrl":"10.1016/j.niox.2025.06.005","url":null,"abstract":"<div><div>Inorganic nitrate plays a crucial role in the regulation of cerebral blood flow and neurotransmission through its conversion to nitric oxide (NO). Astrocytes are star-shaped glial cells and contribute to maintain the blood-brain barrier integrity, regulate neuronal metabolism, support synaptic plasticity and facilitate neurovascular coupling. Inorganic nitrate widely distributed through all organs, with main reservoirs in skeletomuscular and skin tissues. These reserves are easily accessible via bloodstream and processed into nitrite and NO mainly in liver. Processing nitrate/nitrite into NO at organ with main glycogen stores, could suggest an evolutionary coordination between energy metabolism and NO generating pathways. Such spatial arrangement may facilitate the synchronised mobilisation during periods of enhanced metabolic demand, optimising both fuel utilisation and vascular response and assuring optimal fuel distribution. Astrocytes store glycogen in the brain, which support neuronal metabolism during periods of increased neural activity and hypoglycaemia.</div><div>This review explores the hypothesis that inorganic nitrate may be stored alongside glycogen in astrocytes and serve as critical reserves for NO production in the brain, particularly during hypoxic conditions. We examine the emerging evidence that astrocytes serve as key mediators in this alternative nitrate-nitrite-NO pathway, potentially influencing cerebrovascular regulation, neuronal energetics, and cognitive function. The integration of findings across molecular, cellular, and systems neuroscience offers new perspectives on how inorganic nitrate intake might support brain metabolism and could inform both preventive strategies and therapeutic interventions for neuro-degenerative disorders such as age-related dementia, stroke or Parkinson's Disease.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 76-81"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3-mercaptopyruvate sulfurtransferase resides on the inner mitochondrial membrane","authors":"Antonia Katsouda ,&nbsp;Eleni Vasilaki ,&nbsp;Markos Fountoulakis ,&nbsp;Ioanna Tremi ,&nbsp;Sophia Havaki ,&nbsp;Vassilios Myrianthopoulos ,&nbsp;Vassilis G. Gorgoulis ,&nbsp;Emmanuel Mikros ,&nbsp;Andreas Papapetropoulos","doi":"10.1016/j.niox.2025.06.004","DOIUrl":"10.1016/j.niox.2025.06.004","url":null,"abstract":"<div><div>3-mercaptopyruvate sulfurtransferase (MPST) is an enzyme implicated in the generation of the gasotransmitter hydrogen sulfide (H₂S). Unlike, the other two H₂S-synthesizing enzymes cystathionine gamma lyase (CSE) and cystathionine beta synthase (CBS), MPST is found in the mitochondria. However, the mechanisms through which MPST gains access to the mitochondria and its exact localization within this organelle remain unclear. Using immunogold electron microscopy staining, we localized MPST on the inner mitochondrial membrane. To study the pathway of mitochondrial entry for MPST, pharmacological inhibitors of different components of the translocase of outer/inner membrane were used. In line with the observation that ΜPST is found on the inner mitochondrial membrane, inhibition of TIM23 blocked MPST mitochondrial entry. Generation of N-terminally truncated forms of ΜPST did not interfere with the ability of the enzyme to gain access into the mitochondria, suggesting that an N-terminal pre-sequence does not mediate MPST mitochondrial entry. In agreement to this finding, cytosolic and mitochondrial MPST had a similar molecular weight. Interestingly, N-terminally deleted MPST exhibited reduced expression levels, indicating that this part of the enzyme is required for protein stability. Molecular dynamics simulations confirmed that deletion of the first 39 amino acids of the enzyme destabilizes the protein. Our findings reveal that MPST is present on the inner mitochondrial membrane and that its entry into mitochondria does not involve the N-terminus of the protein.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 67-75"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appearances can be deceiving: differences in FeNO values among COPD and severe asthmatic patients stratified according to peripheral eosinophilic count","authors":"Claudio Candia ,&nbsp;Silvestro Ennio D'Anna ,&nbsp;Maria D'Amato ,&nbsp;Francesco Cappello ,&nbsp;Andrea Motta ,&nbsp;Mauro Maniscalco","doi":"10.1016/j.niox.2025.06.003","DOIUrl":"10.1016/j.niox.2025.06.003","url":null,"abstract":"<div><div>Eosinophilic COPD (eCOPD) and eosinophilic severe asthma (eSA) appear to share relevant clinical features, including responsiveness to steroids and higher exacerbation rates. However, data on the expression of T2-high inflammation biomarkers and, in particular comparison of fractional exhaled nitric oxide (FeNO) levels between the two diseases is lacking. The aim of the current retrospective observational study was to investigate whether FeNO values might differ between eCOPD and eSA patients.</div><div>Sixty patients with SA and 40 with COPD were enrolled. They were divided in four groups: eosinophilic COPD (eCOPD) and eosinophilic severe asthma (eSA), if the blood eosinophil count (BEC) was ≥300 cells/μL; non-eosinophilic COPD (neCOPD) and non-eosinophilic severe asthma (neSA) if the BEC was &lt;100 cells/μL. FeNO values, lung function and demographic data were compared between the groups.</div><div>Overall, COPD patients were older, with a higher prevalence of males and had more impaired lung function than asthmatic patients. When comparing FeNO levels among the four groups, a significant difference was found between eCOPD and eSA patients (<em>p</em> = 0.001), as well as eCOPD and neCOPD patients (<em>p =</em> 0.021). Finally, neCOPD patients showed significantly lower FeNO values in comparison with neSA patients (<em>p =</em> 0.005). Such results were confirmed after adjusting for age, sex, and smoking history.</div><div>Our preliminary results hint at the possibility that, despite an apparently similar eosinophilic phenotype, eCOPD patients might present with different FeNO values in comparison with eSA patients, possibly reflecting different underlying disease mechanisms.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 62-66"},"PeriodicalIF":3.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of low nitrate/nitrite diet and nitrate supplementation on litter size and fetal and placental growth in pregnant mice","authors":"Hager M. Kowash ,&nbsp;Teresa Tropea ,&nbsp;Mark R. Dilworth ,&nbsp;Jon O. Lundberg ,&nbsp;Eddie Weitzberg ,&nbsp;Carina Nihlen ,&nbsp;Elizabeth C. Cottrell","doi":"10.1016/j.niox.2025.06.002","DOIUrl":"10.1016/j.niox.2025.06.002","url":null,"abstract":"<div><h3>Background</h3><div>In pregnancy, nitric oxide (NO) plays important roles in embryo development, maternal cardiovascular function, fetoplacental blood flow and fetal growth. Whilst the importance of NO synthase (NOS)-derived NO has been studied in pregnancy, evidence for the role of NO derived from dietary nitrate is lacking. Herein, we utilised dietary manipulation strategies to investigate the effects of dietary nitrate depletion/supplementation on pregnancy outcomes in mice.</div></div><div><h3>Methods</h3><div>Pregnant dams received either a standard control diet or a commercially available nitrate/nitrite depleted (low NOx) diet from the start of pregnancy, along with drinking water containing either 0.7 mM NaNO₃ or 0.7 mM NaCl as control. Pregnancy outcomes (litter size, fetal/placental weights) and maternal and fetal plasma nitrate/nitrite concentrations were determined at gestational day 17.5.</div></div><div><h3>Results and discussion</h3><div>Litter size was unaffected by maternal low NOx diet, but fetal and placental weights were significantly reduced (p &lt; 0.001 for both). Maternal plasma nitrate and nitrite concentrations were significantly lower in low NOx animals (p = 0.017 and p = 0.003, respectively), with maternal 0.7 mM NaNO₃ supplementation restoring nitrate, but not nitrite, levels. Whilst fetal and placental weights were unaffected by maternal NaNO₃ supplementation, litter size was significantly increased (p = 0.024). Unexpectedly, maternal food/energy intake was significantly reduced in low NOx dams (p &lt; 0.001), suggesting that the lower fetal and placental weights may be due to nutrient insufficiency, rather than nitrate depletion <em>per se</em>. These findings have important implications for the interpretation of studies using commercial low NOx diets to study the effects of nitrate-depletion, particularly where food intake has not been previously reported.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 45-51"},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical study on the effect of changes in ostiomeatal complex structure on NO concentration in the nasal cavity","authors":"Shen Yu ,&nbsp;Xiaojun Xie ,&nbsp;Danqing Wang ,&nbsp;Yan Guo ,&nbsp;Jizhe Wang","doi":"10.1016/j.niox.2025.06.001","DOIUrl":"10.1016/j.niox.2025.06.001","url":null,"abstract":"<div><h3>Background</h3><div>Nitric oxide (NO) concentration in the nasal cavity plays an important role in maintaining nasal function and health. We investigated the influence of the geometric structure of the ostiomeatal complex (OMC) on nasal cavity NO concentration distribution and provide optimization strategies for reconstruction surgery related to OMC function.</div></div><div><h3>Method</h3><div>ology: Numerical nasal cavity models of 21 healthy volunteers were established, and the effects of the OMC structure on NO distribution in the nasal cavity were numerically studied. Additionally, the nasal meatus widths of five healthy adult New Zealand rabbits were modified surgically. Exhaled NO concentration was measured pre- and post-surgery to study the effect of nasal meatus width on NO distribution in the rabbit nasal cavities.</div></div><div><h3>Results</h3><div>Through numerical analysis, a quantified relationship was obtained between the width of the middle nasal passage, the hydraulic diameter of the maxillary sinus ostium, and airflow rate with the concentration of NO in the nasal and paranasal sinuses. The impact of these three factors on the NO concentration of exhaled airflow was compared across all volunteers. An optimal combination of middle nasal passage width and ostium diameter can ensure a normal NO concentration in the nasal cavity.</div></div><div><h3>Conclusions</h3><div>When reconstructing the OMC structure to restore function, factors such as the width of the middle nasal meatus and maxillary sinus ostium area and length should be considered comprehensively to reshape an optimal OMC structure for achieving a normal physiological distribution of NO concentration in the nasal cavity.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 38-44"},"PeriodicalIF":3.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic effect of a nitric oxide donor agents based on ruthenium complex combined with cisplatin on lung tumor cell lines","authors":"Angelica E. Graminha ,&nbsp;Amanda B. Becceneri ,&nbsp;Rafaella R Rios ,&nbsp;Márcia Regina Cominetti ,&nbsp;Juliana Cristina Biazzotto ,&nbsp;Roberto Santana da Silva","doi":"10.1016/j.niox.2025.05.009","DOIUrl":"10.1016/j.niox.2025.05.009","url":null,"abstract":"<div><div>Nitric oxide (NO) is a versatile biological messenger involved in numerous physiological processes and anticancer mechanisms. Its functions are highly dependent on its concentration and the specific site of action. In this study, we investigated the effects of controlled NO release mediated by ruthenium-based compounds. The tests demonstrated the significant potential of combining cisplatin with the non-cytotoxic ruthenium nitrosyl complexes <em>cis</em>-[Ru(bpy)₂(NO₂)(solv)]PF₆ and <em>cis</em>-Ru(bpy)₂(NO)(pic)](PF₆)₃, where bpy = 2,2′-bipyridine ,pic = 4-picoline and solv = solvent. This combination increased selectivity between non-tumoral and tumoral lung cells (MRC-5/A549) compared to the selectivity index of cisplatin alone. These nitrosyl complexes exhibited an antagonistic interaction with cisplatin, reducing its cytotoxic efficacy. Cell cycle and apoptosis assays revealed that the cisplatin/Ru combination more effectively inhibited cisplatin's cytotoxic effect on the MRC-5 non-tumoral lung cell line compared to the A549 tumoral cell line. Morphological assays conducted in 3D culture with the <em>cis</em>-[Ru(bpy)₂(NO)(pic)](PF₆)₃ complex confirmed its chemopreventive behavior, as the 3D system closely mimics <em>in vivo</em> conditions. Moreover, the absence of cytotoxicity in these ruthenium nitrosyl complexes highlights their potential as promising candidates for adjuvant therapy in combination with other drugs.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 52-61"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting inducible nitric oxide synthase with 1400W mitigates septic acute lung injury through inhibiting SLC7A11/GPX4 mediated ferroptosis","authors":"Zi-yao Wang ,&nbsp;Tao Zeng ,&nbsp;Xin Wang ,&nbsp;Xin Zhuo ,&nbsp;Jing-wen Zheng ,&nbsp;Ling Zhu ,&nbsp;Shu-ting Cheng ,&nbsp;Li-hong Wan","doi":"10.1016/j.niox.2025.05.008","DOIUrl":"10.1016/j.niox.2025.05.008","url":null,"abstract":"<div><div>Accumulating evidence has indicated that lung ferroptosis is an important contributor to septic acute lung injury (SALI). Inducible nitric oxide synthase (iNOS) may be implicated in the regulation of bronchial epithelial ferroptosis. Nevertheless, the precise mechanisms by which iNOS modulates ferroptosis remain elusive. This study investigated whether iNOS selective inhibitor 1400w alleviates LPS-induced SALI and suppresses ferroptosis in mice. Additionally, RNA sequencing (RNA-seq), molecular docking, molecular dynamic simulation, Transmission electron microscope (TEM), and western blotting were employed to predict and evaluate the molecular mechanism of 1400w on LPS-induced ferroptosis in vivo. The results showed that the administration of 1400w markedly attenuated LPS-induced lung injury and facilitated pulmonary function in mice. Also, 1400w administration effectively suppressed bronchial epithelial ferroptosis induced by LPS in mice. Furthermore, molecular docking and molecular dynamics simulations revealed stable binding between GPX4 and iNOS, with 1400w modulating ferroptosis mediated by SLC7A11/GPX4 through targeting iNOS. Collectively, our research demonstrated that inhibition of iNOS might represent a potential therapeutic strategy to improve SALI by inhibiting ferroptosis.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 4-10"},"PeriodicalIF":3.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation of S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine stability in biomedical contexts","authors":"Qingqing Fan ,&nbsp;Shu Geng ,&nbsp;Olivia Rusli ,&nbsp;Federico Mazur ,&nbsp;Zifei Han ,&nbsp;Nicole Joy Rijs ,&nbsp;Rona Chandrawati","doi":"10.1016/j.niox.2025.05.006","DOIUrl":"10.1016/j.niox.2025.05.006","url":null,"abstract":"<div><div>Nitric oxide (NO) is a potent signaling molecule with great therapeutic potential. However, the clinical application of direct NO delivery is limited by its short half-life and high reactivity, which creates challenges for effective controlled delivery. To overcome these limitations, <em>S</em>-nitrosothiols (RSNOs) are commonly used as NO donors in therapeutic applications, as they stabilize the short-lived free radical and improve NO pharmacokinetics. Despite their widespread use, the stability and release kinetics of RSNOs under physiological conditions have not been thoroughly evaluated, which is crucial for determining their therapeutic efficacy and safety. This study provides a comprehensive evaluation of the stability and <em>in vitro</em> NO release profiles of two commonly used RSNOs, <em>S</em>-nitrosoglutathione (GSNO) and <em>S</em>-nitroso-<em>N</em>-acetylpenicillamine (SNAP), at physiologically relevant conditions. Using a range of analytical techniques, including electrospray ionization mass spectrometry, Griess assay, and electrochemical sensing, we assessed RSNO stability across various conditions, including different buffers, pH levels, temperatures, as well as exposure to UV light irradiation to simulate common sterilization practices. Additionally, we investigated RSNO stability in cell culture media with varying glucose levels and serum compositions to better mimic biological environments. Our findings provide critical insights into the factors affecting RSNO stability and NO release, advancing the development of more effective NO-based therapies and biomedical devices.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 27-37"},"PeriodicalIF":3.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of acute nitrate supplementation on the superficial conduit venous vascular response at rest and during sympathoexcitation","authors":"Anna Oue,&nbsp;Momo Ito,&nbsp;Yasuhiro Iimura,&nbsp;Naho Serizawa,&nbsp;Yuichi Miyakoshi,&nbsp;Masako Ota","doi":"10.1016/j.niox.2025.05.007","DOIUrl":"10.1016/j.niox.2025.05.007","url":null,"abstract":"<div><div>The effects of increased nitric oxide (NO) activity and/or NO precursor levels following nitrate (NO₃⁻) supplementation on venous vascular control remain poorly understood. We investigated the effect of acute NO₃⁻ supplementation on the venous vascular response of a single conduit vein at rest and during sympathoexcitation (e.g., static exercise and muscle metaboreflex). Participants were 15 healthy young adults who consumed either beetroot juice (BRJ, 140 mL; ∼8 mmol NO₃⁻) or a control beverage (prune juice; CON, 166 mL; &lt;0.01 mmol NO₃⁻) following a random crossover study design. Two hours after consuming the allocated beverage, each participant rested for 4 min and then performed a continuous isometric forearm exercise (forearm exercise) using the right arm at 45 % of their maximal voluntary constriction for 1.5 min, followed by a 2-min recovery period with arterial occlusion of the exercising arm to activate the muscle metaboreflex only. Mean arterial pressure (MAP), heart rate (HR), and the cross-sectional area of the superficial vein in the left non-exercising arm (CSA_{<em>vein</em>}) were measured. BRJ intake increased the plasma NO₃⁻ concentration (p &lt; 0.05). All resting parameters were similar with CON and BRJ. MAP and HR increased and the CSA_{<em>vein</em>} decreased with exercise (p &lt; 0.05), and these changes were maintained during recovery, except for HR. The increase in MAP was lower in the BRJ group than in the CON group (p &lt; 0.05), although the magnitude of the CSA_{<em>vein</em>} decrease did not differ between the groups. These findings suggest that, in a single conduit vein, the increasing NO precursors by BRJ intake does not alter either the venous vascular tone at rest or the sympathetic venoconstriction during forearm exercise or during the activation of muscle metaboreceptors.</div></div>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":"158 ","pages":"Pages 18-24"},"PeriodicalIF":3.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}