{"title":"A Versatile Vanadium(III)-Based Chemiluminescence Protocol for Nitric Oxide Metabolite Quantification and NO Release Kinetics.","authors":"Joseph A Bauer, Annette M Sysel, Michael J Dunphy","doi":"10.1016/j.niox.2025.10.006","DOIUrl":null,"url":null,"abstract":"<p><p>Nitric oxide (NO) is a central mediator of vascular, neurological, and immune functions, and its dysregulation is implicated in a broad spectrum of diseases. Despite its significance, direct measurement of NO in human samples is limited by its transient nature and rapid conversion to nitrate and nitrite. Accurate, rapid, and accessible quantification of these NO metabolites in clinical and research settings remains a key need. We present a vanadium(III)-based chemiluminescence protocol for the reliable detection of nitrate and nitrite in human biological fluids, including serum, urine, and cerebrospinal fluid. In addition, this method supports headspace gas analysis, enabling precise determination of nitric oxide release kinetics and half-life from NO-producing compounds. This approach offers a cost-effective and scalable solution suitable for routine analysis in both diagnostic and research laboratories.</p>","PeriodicalId":19357,"journal":{"name":"Nitric oxide : biology and chemistry","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nitric oxide : biology and chemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.niox.2025.10.006","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Nitric oxide (NO) is a central mediator of vascular, neurological, and immune functions, and its dysregulation is implicated in a broad spectrum of diseases. Despite its significance, direct measurement of NO in human samples is limited by its transient nature and rapid conversion to nitrate and nitrite. Accurate, rapid, and accessible quantification of these NO metabolites in clinical and research settings remains a key need. We present a vanadium(III)-based chemiluminescence protocol for the reliable detection of nitrate and nitrite in human biological fluids, including serum, urine, and cerebrospinal fluid. In addition, this method supports headspace gas analysis, enabling precise determination of nitric oxide release kinetics and half-life from NO-producing compounds. This approach offers a cost-effective and scalable solution suitable for routine analysis in both diagnostic and research laboratories.
期刊介绍：
Nitric Oxide includes original research, methodology papers and reviews relating to nitric oxide and other gasotransmitters such as hydrogen sulfide and carbon monoxide. Special emphasis is placed on the biological chemistry, physiology, pharmacology, enzymology and pathological significance of these molecules in human health and disease. The journal also accepts manuscripts relating to plant and microbial studies involving these molecules.
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