Nucleosides, Nucleotides & Nucleic Acids最新文献

筛选
英文 中文
Clinical significance of LIN28A gene polymorphisms and expression in pan-cancer: a meta-analysis and bioinformatic analysis. 泛癌症中 LIN28A 基因多态性和表达的临床意义:一项荟萃分析和生物信息学分析。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-08-18 DOI: 10.1080/15257770.2024.2393316
Surui Zhou, Jinyin Xue, Qijun Yang, Wenjing Zang, Yi Chen, Yining Zhao, Xueren Gao
{"title":"Clinical significance of <i>LIN28A</i> gene polymorphisms and expression in pan-cancer: a meta-analysis and bioinformatic analysis.","authors":"Surui Zhou, Jinyin Xue, Qijun Yang, Wenjing Zang, Yi Chen, Yining Zhao, Xueren Gao","doi":"10.1080/15257770.2024.2393316","DOIUrl":"10.1080/15257770.2024.2393316","url":null,"abstract":"<p><p>Several studies have reported the relationship between <i>LIN28A</i> gene polymorphisms (rs3811463 T > C and rs34787247 G > A) and cancer susceptibility, but the results are inconsistent and need further clarification. The current study aimed to evaluate their relationship and also to explore the relationship between <i>LIN28A</i> gene expression and immune infiltration, tumor stage, survival prognosis, and drug sensitivity in pan-cancer. The meta-analysis and data mining were completed by STATA software and the GSCA platform, respectively. The meta-analysis showed that the rs3811463 polymorphism was not associated with cancer susceptibility, while the rs34787247 polymorphism was associated with cancer susceptibility in the Chinese population [AA vs. GG: Odd Ratio (OR)=1.98, 95% Confidence Interval (CI)=1.35-2.89, P<sub>Z</sub><0.001; GA vs. GG: OR = 1.17, 95%CI= 1.01-1.36, P<sub>Z</sub>=0.04; (AA + GA) vs. GG: OR = 1.24, 95%CI = 1.07-1.43, P<sub>Z</sub>=0.004; AA vs. (GA + GG): OR = 1.90, 95%CI = 1.30- 2.78, P<sub>Z</sub>=0.001; A vs. G: OR = 1.27, 95%CI = 1.12-1.44, P<sub>Z</sub><0.001]. <i>LIN28A</i> gene expression was associated not only with immune infiltration, pathological stage, and survival prognosis of certain cancers, but also with sensitivity to multiple anticancer drugs, such as cisplatin, pazopanib, olaparib, and selumetinib. In conclusion, the current study suggested that the rs34787247 G > A polymorphism might be used as a cancer risk marker in the Chinese population, and LIN28A might serve as a prognostic marker and therapeutic target for certain cancers.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"643-652"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between genetic variants (rs2839698, and rs217727) in lncRNA H19 and Acute lymphoblastic leukemia susceptibility: a case-control study in the Iranian population. lncRNA H19 基因变异(rs2839698 和 rs217727)与急性淋巴细胞白血病易感性之间的关系:一项在伊朗人群中进行的病例对照研究。
IF 1.3 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-10-29 DOI: 10.1080/15257770.2024.2422007
Paria Farhadian, Mohammad Javad Mokhtari
{"title":"Association between genetic variants (rs2839698, and rs217727) in <i>lncRNA H19</i> and Acute lymphoblastic leukemia susceptibility: a case-control study in the Iranian population.","authors":"Paria Farhadian, Mohammad Javad Mokhtari","doi":"10.1080/15257770.2024.2422007","DOIUrl":"10.1080/15257770.2024.2422007","url":null,"abstract":"<p><p>Leukemia is a cancer affecting the hematopoietic system with an unclear pathogenesis. Recent studies suggest a correlation between several long non-coding RNAs (lncRNAs) and leukemia development. This study focuses on the potential link between <i>H19</i> (rs2839698 and rs217727) polymorphisms and Acute Lymphoblastic Leukemia (ALL) susceptibility. The study involved 150 patients with clinically confirmed ALL and 150 controls. This research included 150 Iranian patients, who were confirmed to have clinical ALL, and 150 healthy people as the control group. A kit was utilized to extract the DNA of all the samples. After preparing the samples, DNA genotyping was done by using the tetra-primer ARMS-PCR method. After adjusting for age using multivariate logistic regression analysis, individuals carrying the CT genotype of rs2839698 were found to have a significantly 0.32-fold reduced risk of ALL compared with carriers of the CC genotype. Furthermore, a significant 0.48-fold reduction in ALL risk was observed in patients with CT+TT genotype rs2839698 compared with CC. Moreover, the over-dominant model was applied to compare the CT genotype of rs2839698 with its CC+TT genotype, which showed a significant 0.36-fold reduction of ALL risk. Notably, the cases of ALL and the control group were not significantly different in terms of their genotype and allele frequencies of rs217727 polymorphism. Yet, the TT haplotype was significantly associated with ALL risk (OR: 1.64, <i>p</i> = 0.025). Following the findings of this study, it can be concluded that <i>H19</i> SNP rs2839698, rather than rs217727, might act as an innovative susceptibility marker for ALL leukemia.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"793-807"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Escherichia coli Orf135 (NudG) mutant protein specific for oxidized dATP. 大肠杆菌 Orf135(NudG)突变蛋白对氧化 dATP 的特异性。
IF 1.3 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-10-11 DOI: 10.1080/15257770.2024.2413878
Hiroyuki Kamiya
{"title":"<i>Escherichia coli</i> Orf135 (NudG) mutant protein specific for oxidized dATP.","authors":"Hiroyuki Kamiya","doi":"10.1080/15257770.2024.2413878","DOIUrl":"10.1080/15257770.2024.2413878","url":null,"abstract":"<p><p>Damaged 2'-deoxyribonucleotides cause mutations, cancer, cell death, and aging. The <i>Escherichia coli</i> Orf135 (NudG) protein catalyzes the hydrolysis of various 2'-deoxyribonucleotides including an oxidized form of dATP, 2-oxo-1,2-dihydro-2'-deoxyadenosine 5'-triphosphate (dA<sup>O</sup>TP, 2-hydroxy-2'-deoxyadenosine 5'-triphosphate). The best substrate is 5-methyl-2'-deoxycytidine 5'-triphosphate (dC<sup>m</sup>TP), and the protein prefers dC<sup>m</sup>TP over dA<sup>O</sup>TP by ∼200-fold in vitro. To make the enzyme specific for the mutagenic nucleotide dA<sup>O</sup>TP, a double mutant protein (E33A plus D118E) was designed and produced in <i>E. coli</i>. The purified mutant protein showed one order of magnitude higher dA<sup>O</sup>TP preference over dC<sup>m</sup>TP. The split protein based on this mutant may potentially be used to detect dA<sup>O</sup>TP in living cells.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"886-893"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prognostic value and immunological role of MMRN1: a rising star in cancer. MMRN1 的预后价值和免疫学作用:癌症中的一颗新星。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-05-07 DOI: 10.1080/15257770.2024.2335680
Qing Zhou, Ying Liu, Jieyu Zhou, Wenling Zhang
{"title":"Prognostic value and immunological role of MMRN1: a rising star in cancer.","authors":"Qing Zhou, Ying Liu, Jieyu Zhou, Wenling Zhang","doi":"10.1080/15257770.2024.2335680","DOIUrl":"10.1080/15257770.2024.2335680","url":null,"abstract":"<p><strong>Background: </strong>Multimerin 1 (MMRN1) is a factor V binding protein, which can support platelet adhesion and thrombus formation. In recent years, the role of MMRN1 in cancer has begun to attract attention. But systematic studies in this area are lacking. Therefore, we used bioinformatics methods to analyze MMRN1 in tumors to reveal the possible role of MMRN1.</p><p><strong>Methods: </strong>Using the Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) database, we obtained relevant data for analyzing MMRN1. Using Gene Expression Profiling Interactive Analysis (GEPIA), Human Protein Atlas (HPA), TCGA, GeneMANIA, and cBioPortal, we explored the potential role of MMRN1 in different types of tumors. Tumor Immune System Interactions and Drug Bank (TISIDB) and Sangerbox were used to analyze the correlation between MMRN1 and tumor immunity. Gene set cancer analysis (GSCA) and UALCAN were used to analyze the methylation of MMRN1. GSCA was also used to analyze the drug sensitivity of MMRN1.</p><p><strong>Results: </strong>MMRN1 is down-regulated in most cancer types and is closely related to the prognosis of cancer patients. Interestingly, in most tumors, MMRN1 is positively correlated with immune -related genes. In addition, we observed different levels of methylation and mutations in different types of tumors. Drug sensitivity analysis found that MMRN1 was negatively correlated with several drugs, including GW-2580 and TL-1-85, suggesting that it can be used to develop potential anticancer therapies.</p><p><strong>Conclusion: </strong>Our analysis demonstrated a significant relationship between MMRN1 and prognosis, tumor immunity, and drug sensitivity of several tumors. As a rising star in cancer, it needs further research.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"148-169"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The critical role and functional mechanism of microRNA-146a in doxorubicin-induced apoptosis in breast cancer cells. microRNA-146a在多柔比星诱导乳腺癌细胞凋亡中的关键作用和功能机制
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-03-26 DOI: 10.1080/15257770.2024.2330592
Sara Tutunchi, Parisa Nourmohammadi, Roghayeh Tofigh, Saeedeh Akhavan, Mina Zare, Sadra Samavarchi Tehrani, Ghodratollah Panahi
{"title":"The critical role and functional mechanism of microRNA-146a in doxorubicin-induced apoptosis in breast cancer cells.","authors":"Sara Tutunchi, Parisa Nourmohammadi, Roghayeh Tofigh, Saeedeh Akhavan, Mina Zare, Sadra Samavarchi Tehrani, Ghodratollah Panahi","doi":"10.1080/15257770.2024.2330592","DOIUrl":"10.1080/15257770.2024.2330592","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer among women is the most frequently diagnosed cancer and the leading cause of death worldwide. There many advances in diagnosing and treating this disease, early diagnosis and treatment are still a significant challenge in the early stages. In recent years, microRNAs have attracted much attention in cancer diagnosis and treatment. However, the role of miR-146a in breast cancer is still controversial. We aimed to investigate the roles of miR-146a in apoptosis in breast cancer cells.</p><p><strong>Methods: </strong>A microarray dataset from the GEO database was selected, and using the GEO2R tool, the gene expression profile of this dataset was extracted. Then, the target scan database was used to explore the miR-146a target genes. The link between the signaling pathways was collected. We used miR-146a mimic, which was transfected to the MCF-7 cells to investigate the miR-146a roles in the apoptosis. The expression levels of miR-146a and BAX, BCL-2, and p-21(most essential genes in the apoptosis) were quantified by qPCR and western blot analysis.</p><p><strong>Results: </strong>Our findings indicated that doxorubicin induces miR-146a expression. In addition, overexpression of miR-146a affected MCF-7 cell viability, induced apoptosis, and led to reduced expression levels of BCL-2 and P-21, as well as increased BAX expression levels.</p><p><strong>Conclusion: </strong>Considering the role of doxorubicin in inducing apoptosis and increasing the expression of miR-146a, it can be suggested that this miR is involved in inducing apoptosis in BC cells. In addition, miR-146a can be considered a therapeutic candidate.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"124-135"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HLAB27 may confer protection to COVID-19 in generalized vitiligo patients from South Gujarat population. 在南古吉拉特人群中,HLAB27 可能会对泛发性白癜风患者的 COVID-19 产生保护作用。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-01-19 DOI: 10.1080/15257770.2024.2303710
Prashant S Giri, Radhika Bhimani, Naresh C Laddha, Mitesh Dwivedi
{"title":"<i>HLAB27</i> may confer protection to COVID-19 in generalized vitiligo patients from South Gujarat population.","authors":"Prashant S Giri, Radhika Bhimani, Naresh C Laddha, Mitesh Dwivedi","doi":"10.1080/15257770.2024.2303710","DOIUrl":"10.1080/15257770.2024.2303710","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), while generalized vitiligo(GV) is an autoimmune disease that causes the loss of functional melanocytes, resulting in white patches all over the body. Human Leukocyte Antigen (HLA) plays a crucial role in immune response to pathogens. Studies assessing the link between GV and COVID-19 are lacking; therefore, our current study was aimed to establish the association between GV and <i>HLAB27</i> by genotyping the <i>HLAB27</i> allele in 150 GV patients and 150 controls from South Gujarat population through polymerase chain reaction-sequence-specific primers (PCR-SSP) method. Additionally, we assessed the correlation of GV with COVID-19 and the influence of <i>HLAB27</i> on COVID-19 development. Interestingly, our study suggested that the <i>HLAB27</i> allele was prevalent in GV patients as compared to controls (52% <i>vs</i> 35.33%; <i>p</i> = 0.0051). Moreover, the occurrence of COVID-19 was significantly lower in GV patients than in controls (10% <i>vs</i> 32.66%; <i>p</i> < 0.0001). Disease activity-based analysis suggested that COVID-19 occurrence was significantly lower in active vitiligo (AV) patients as compared to stable vitiligo (SV) patients(6.87% <i>vs</i> 31.57%; <i>p</i> = 0.0045). Furthermore, COVID-19 development was significantly reduced in <i>HLAB27</i> positive individuals as compared to <i>HLAB27</i> negative individuals (<i>p</i> = 0.0025). Overall, our study suggests, for the first time, that <i>HLAB27</i> allele might be a genetic risk factor for GV susceptibility, and an ongoing immune response in GV patients, more specifically in AV patients, might protect against COVID-19 infection in South Gujarat population. Additionally, our study highlighted the likely role of <i>HLAB27</i> in protection against COVID-19 development.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of polymorphisms within P2RX4 with type 2 diabetes mellitus: a preliminary case-control study. P2RX4 多态性与 2 型糖尿病的关系:一项初步病例对照研究。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-07-02 DOI: 10.1080/15257770.2024.2373300
Homa Noori, Mahdi Majidpour, Mahboobeh Sabeti Akbar-Abad, Ramin Saravani
{"title":"Association of polymorphisms within <i>P2RX4</i> with type 2 diabetes mellitus: a preliminary case-control study.","authors":"Homa Noori, Mahdi Majidpour, Mahboobeh Sabeti Akbar-Abad, Ramin Saravani","doi":"10.1080/15257770.2024.2373300","DOIUrl":"10.1080/15257770.2024.2373300","url":null,"abstract":"<p><strong>Objective: </strong>Type 2 diabetes mellitus (T2DM) is a complex heterogenic metabolic with a wide range of etiology. Purinergic receptors have pivotal roles in different processes and are hypothesized to have roles in the pathogenesis of T2DM.</p><p><strong>Materials and methods: </strong>Three hundred subjects affected by T2DM and 300 healthy subjects were genotyped by amplification refractory mutation system polymerase chain reaction (ARMS-PCR). SPSS V16.0 was recruited for statistical analysis.</p><p><strong>Results: </strong>The findings showed that the G allele of rs25644A > G increases the risk of T2DM in our population statistically (OR = 1.51, 95% CI = 1.14-1.99, <i>p</i> = 0.003). This allele in some genotype models, including the dominant model, caused an increase in the risk of T2DM. The interaction of genotypes between studied variants in the <i>P2XR4</i> gene increased the risk of T2DM. Haplotype analysis showed that A<sub>rs1169727</sub>/G<sub>rs25644</sub> haplotype caused an increase in the risk of T2DM.</p><p><strong>Conclusions: </strong>The findings suggest that rs25644A > G plays a role in our population's increased risk of T2DM.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"397-407"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Current updates on genetic spectrum of usher syndrome. 目前有关 usher 综合征遗传谱的最新进展。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-05-08 DOI: 10.1080/15257770.2024.2344194
Farman Ullah, Muhammad Zeeshan Ali, Safeer Ahmad, Muhammad Muzammal, Saadullah Khan, Jabbar Khan, Muzammil Ahmad Khan
{"title":"Current updates on genetic spectrum of usher syndrome.","authors":"Farman Ullah, Muhammad Zeeshan Ali, Safeer Ahmad, Muhammad Muzammal, Saadullah Khan, Jabbar Khan, Muzammil Ahmad Khan","doi":"10.1080/15257770.2024.2344194","DOIUrl":"10.1080/15257770.2024.2344194","url":null,"abstract":"<p><p>Usher syndrome (USH) is a genetic disorder that is characterized by sensorineural hearing loss (HL) and visual abnormality, i.e., loss of night vision and side (peripheral) vision. Usher syndrome is categorized into four subtypes (USH1, USH2, USH3, USH4) on the basis of phenotypic spectrum. Profound hearing loss (HL), vestibular are flexia and language disturbance are typically associated with Usher type 1, while USH2 is linked with moderate to severe level of congenital HL. USH3 has late onset of deafness in life (referred to as \"postlingual\"), inconstant vestibular abnormality and onset of retinitis pigmentosa (RP) typically in 2nd decade of life. Patients with USH4 have no vestibular impairment and have late onset of retinitis pigmentosa (RP) and sensorineural hearing loss. Until now, 15 genetic loci have been reported to be linked with all types of USH. Among reported USH loci, nine are related to be involved in USH1, three in USH2, two in USH3 and one locus in USH4, respectively. Current review has described different types of Usher syndrome and their molecular genetics, and role of usher proteins in sensory organs. Moreover, we also suggested certain candidate genes for uncharacterized loci that may help the molecular geneticist to reach their target easily. Conclusion: The current catalogue of USH genetic data may assist in genetic counseling, genetic diagnosis, and genotype-phenotype correlation.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"337-360"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nucleic acids based integrated macromolecular complexes for SiRNA delivery: Recent advancements. 用于 SiRNA 递送的基于核酸的集成大分子复合物:最新进展。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-05-01 DOI: 10.1080/15257770.2024.2347499
Dilpreet Singh, Lovedeep Singh, Simranjeet Kaur, Akshita Arora
{"title":"Nucleic acids based integrated macromolecular complexes for SiRNA delivery: Recent advancements.","authors":"Dilpreet Singh, Lovedeep Singh, Simranjeet Kaur, Akshita Arora","doi":"10.1080/15257770.2024.2347499","DOIUrl":"10.1080/15257770.2024.2347499","url":null,"abstract":"<p><p>The therapeutic potential of small interfering RNA (siRNA) is monumental, offering a pathway to silence disease-causing genes with precision. However, the delivery of siRNA to target cells <i>in-vivo</i> remains a formidable challenge, owing to degradation by nucleases, poor cellular uptake and immunogenicity. This overview examines recent advancements in the design and application of nucleic acid-based integrated macromolecular complexes for the efficient delivery of siRNA. We dissect the innovative delivery vectors developed in recent years, including lipid-based nanoparticles, polymeric carriers, dendrimer complexes and hybrid systems that incorporate stimuli-responsive elements for targeted and controlled release. Advancements in bioconjugation techniques, active targeting strategies and nanotechnology-enabled delivery platforms are evaluated for their contribution to enhancing siRNA delivery. It also addresses the complex interplay between delivery system design and biological barriers, highlighting the dynamic progress and remaining hurdles in translating siRNA therapies from bench to bedside. By offering a comprehensive overview of current strategies and emerging technologies, we underscore the future directions and potential impact of siRNA delivery systems in personalized medicine.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"409-432"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of exon regions in eukaryotes using fine-tuned variational mode decomposition based on kurtosis and short-time discrete Fourier transform. 利用基于峰度和短时离散傅立叶变换的微调变异模式分解技术识别真核生物中的外显子区域。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-08-10 DOI: 10.1080/15257770.2024.2388785
K Jayasree, Malaya Kumar Hota, Atul Kumar Dwivedi, Himanshuram Ranjan, Vinay Kumar Srivastava
{"title":"Identification of exon regions in eukaryotes using fine-tuned variational mode decomposition based on kurtosis and short-time discrete Fourier transform.","authors":"K Jayasree, Malaya Kumar Hota, Atul Kumar Dwivedi, Himanshuram Ranjan, Vinay Kumar Srivastava","doi":"10.1080/15257770.2024.2388785","DOIUrl":"10.1080/15257770.2024.2388785","url":null,"abstract":"<p><p>In genomic research, identifying the exon regions in eukaryotes is the most cumbersome task. This article introduces a new promising model-independent method based on short-time discrete Fourier transform (ST-DFT) and fine-tuned variational mode decomposition (FTVMD) for identifying exon regions. The proposed method uses the <i>N</i>/3 periodicity property of the eukaryotic genes to detect the exon regions using the ST-DFT. However, background noise is present in the spectrum of ST-DFT since the sliding rectangular window produces spectral leakage. To overcome this, FTVMD is proposed in this work. VMD is more resilient to noise and sampling errors than other decomposition techniques because it utilizes the generalization of the Wiener filter into several adaptive bands. The performance of VMD is affected due to the improper selection of the penalty factor (<i>α</i>), and the number of modes (<i>K</i>). Therefore, in fine-tuned VMD, the parameters of VMD (<i>K</i> and <i>α</i>) are optimized by maximum kurtosis value. The main objective of this article is to enhance the accuracy in the identification of exon regions in a DNA sequence. At last, a comparative study demonstrates that the proposed technique is superior to its counterparts.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"507-530"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信