Nucleosides, Nucleotides & Nucleic Acids最新文献_第8页

Revolutionizing DNA: advanced modification techniques for next-gen nanotechnology. 革新 DNA：新一代纳米技术的先进修饰技术。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-26 DOI: 10.1080/15257770.2024.2432992

Pratikeswar Panda, Rajaram Mohapatra

{"title":"Revolutionizing DNA: advanced modification techniques for next-gen nanotechnology.","authors":"Pratikeswar Panda, Rajaram Mohapatra","doi":"10.1080/15257770.2024.2432992","DOIUrl":"https://doi.org/10.1080/15257770.2024.2432992","url":null,"abstract":"The comprehensive advancement in DNA modification and coupling is driving DNA nanotechnology to new heights, paving the way for groundbreaking innovations in healthcare, materials science, and beyond. The ability to engineer DNA with tailored properties and functionalities underscores its immense potential in creating novel materials and devices. Utilizing a spectrum of techniques-such as amino handles, thiol groups, alkynes, azides, Diels-Alder reactions, hydrazides, and aminooxy functions-enables diverse coupling strategies, including Palladium-Catalyzed Couplings, to construct intricate DNA nanostructures. Further coupling modifications encompass hydrophobic alterations, redox-active moieties, chemical crosslinking agents, and Biotinylation. These modifications significantly broaden DNA's functional repertoire, offering precise control over interactions, structures, and features. By leveraging these advanced techniques, alongside next-generation sequencing (NGS)-based DNA modifications, researchers can design and implement DNA nanostructures with specific capabilities and applications, showcasing DNA's versatility as a programmable biomaterial. Through meticulous design and strategic implementation, DNA nanotechnology achieves unprecedented levels of precision and functionality, ushering in a new era of technological advancements and applications. These advanced DNA modification techniques hold great potential for transformative applications in nanotechnology, paving the way for innovations in drug delivery, diagnostics, and bioengineering.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-32"},"PeriodicalIF":1.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical relevance and function of HMGB1 gene polymorphism and expression in colorectal cancer. 结直肠癌中 HMGB1 基因多态性和表达的临床相关性及功能。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-26 DOI: 10.1080/15257770.2024.2432991

Fang Wang, Zhijun Huang, Jianping Li, Xueren Gao

{"title":"Clinical relevance and function of HMGB1 gene polymorphism and expression in colorectal cancer.","authors":"Fang Wang, Zhijun Huang, Jianping Li, Xueren Gao","doi":"10.1080/15257770.2024.2432991","DOIUrl":"https://doi.org/10.1080/15257770.2024.2432991","url":null,"abstract":"High HMGB1 levels contribute to the development and metastasis of tumors such as colorectal cancer (CRC). The current investigation sought to evaluate the association of a functional InDel polymorphism (rs34000982) on the HMGB1 gene with CRC susceptibility and tumor stage and the clinical relevance of HMGB1 gene expression. A total of 600 CRC patients and 600 healthy control individuals were genotyped by a polymerase chain reaction-polyacrylamide gel electrophoresis assay. The findings demonstrated that the rs34000982 Ins allele or Ins/Ins genotype was associated not only with reduced susceptibility to CRC, especially stage III-IV CRC (Ins vs. Del: OR = 0.65, 95%CI = 0.51-0.82, p < 0.001; Ins/Ins vs. Del/Del: OR = 0.29, 95%CI = 0.14- 0.60, p < 0.001), but also with tumor stage. CRC patients carrying the Ins allele or Ins/Ins genotype had a significantly lower risk of stage III-IV tumors (Ins vs. Del: OR = 0.69, 95%CI = 0.53- 0.91; Ins/Ins vs. Del/Del: OR = 0.41, 95%CI = 0.18-0.94). Functional research revealed that the rs34000982 Ins allele enabled hsa-miR-944 to interact with the 3' untranslated region of HMGB1. In addition, HMGB1 gene expression levels were associated not only with multiple immune cell infiltration, but also with multiple anti-CRC drug sensitivities. The current findings suggest that the HMGB1 rs34000982 polymorphism may serve as a marker of CRC susceptibility and progression in the Chinese population, and HMGB1 levels may serve as an anti-CRC drug sensitivity marker.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic determinants in pathogenicity of SARS-CoV-2 versa common cold coronaviruses. SARS-CoV-2 与普通感冒冠状病毒致病性的基因组决定因素。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-21 DOI: 10.1080/15257770.2024.2430397

Zahra Arab-Bafrani, Majid Nikoubin-Boroujeni, Saeedeh Ebrahimi, Ali Teimoori, Elham Heidari, Elham Mousavi

{"title":"Genomic determinants in pathogenicity of SARS-CoV-2 versa common cold coronaviruses.","authors":"Zahra Arab-Bafrani, Majid Nikoubin-Boroujeni, Saeedeh Ebrahimi, Ali Teimoori, Elham Heidari, Elham Mousavi","doi":"10.1080/15257770.2024.2430397","DOIUrl":"https://doi.org/10.1080/15257770.2024.2430397","url":null,"abstract":"Determination of the different short oligonucleotide features in the full genome of fatal and mild coronavirus strains can show the researchers how these viruses evolved and became virulent strains. To this aim, at first, in the full genome of all coronavirus strains included in this study, the observed and expected frequency of dinucleotide to hexanucleotide was obtained using Markov method. Then odds ratio (observed/expected abundances) of short oligonucleotide was computed and considered as the raw data (features). Finally, ten distinct weighting algorithms approaches (Information Gain, Information Gain Ratio, Rule, Deviation, Chi Squared, Gini Index, Uncertainty, Relief, Support Vector Machine (SVM), and PCA) was employed on the features to identify oligonucleotide distribution differences across the full genome of SARS-related viruses compared to common cold coronaviruses. Totally among 5440 features (16 dinucleotides, 64 trinucleotides, 256 tetra nucleotides, 1024 penta-nucleotides, and 4096 Hexa-nucleotides), CC, CCA, CCAC, ACCAC, and CACCAC motifs were selected by 80 -90% of all weighting algorithms models to distinguish virulent strains from mild coronaviruses. These remarkable oligonucleotides might point toward the existence of some particular RNA elements that might be involved in viral virulence and thus can be targeted for viral treatment in the future.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotransmitters and neural hormone-based probes for quadruplex DNA sequences associated with neurodegenerative diseases. 基于神经递质和神经激素的探针，用于检测与神经退行性疾病相关的四重 DNA 序列。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-19 DOI: 10.1080/15257770.2024.2431145

Callie Rohrer, Alexis Palumbo, Marissa Paul, Erin Reese, Swarna Basu

引用次数: 0

Design and synthesis of fluorescence-labeled nucleotide with a cleavable aryl-triazene linker for DNA sequencing by synthesis. 设计并合成带有可裂解芳基三氮烯连接体的荧光标记核苷酸，用于 DNA 测序合成。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-18 DOI: 10.1080/15257770.2024.2429696

Bo-Wei Tang, Ping-Yang Wang, Yu-Mei Shen

引用次数: 0

Bioinformatics analysis of key genes and potential therapeutic agents for vascular calcification in chronic kidney disease. 慢性肾病血管钙化关键基因和潜在治疗药物的生物信息学分析。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-18 DOI: 10.1080/15257770.2024.2423214

Guojie Ye, Tengfei Liu, Chunhua Ding

引用次数: 0

DNA damage-inducing endogenous and exogenous factors and research progress. DNA 损伤诱导内源性和外源性因素及研究进展。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-14 DOI: 10.1080/15257770.2024.2428436

Nian Liu, Jiahui Du, Jiani Ge, Song-Bai Liu

引用次数: 0

Harmine promotes odontoblastic differentiation of dental pulp stem cells. Harmine 可促进牙髓干细胞的牙髓分化。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-14 DOI: 10.1080/15257770.2024.2427930

Touraj Zamir Nasta, Mohammad Reza Tabandeh, Ardeshir Abbasi, Hiva Moradi, Mohammad Moslem Imani, Cyrus Jalili

{"title":"Harmine promotes odontoblastic differentiation of dental pulp stem cells.","authors":"Touraj Zamir Nasta, Mohammad Reza Tabandeh, Ardeshir Abbasi, Hiva Moradi, Mohammad Moslem Imani, Cyrus Jalili","doi":"10.1080/15257770.2024.2427930","DOIUrl":"https://doi.org/10.1080/15257770.2024.2427930","url":null,"abstract":"Introduction: Dental pulp stem cells (DPSCs) have the potential to differentiate into various types of tissues including tooth, adipose, cartilage, muscle, nerve, and also possess regenerative properties. Harmine, a beta-carboline alkaloid, has been shown to have antitumor activities and promote bone formation through the differentiation of osteoblasts. The aim of this study was to investigate the effect of harmine on the differentiation of DPSCs into odontoblast cells.Materials and methods: DPSCs were obtained from Iran's National Genetic Reserve Center and cultured under standard stem cell culture conditions. The cells were differentiated in culture medium with and without harmine, and cell viability was evaluated using MTT assay at different harmine concentrations. Moreover, differentiation of cells was measured using Alizarin Red staining, and the expression of Runx2, DSPP, and DMP1 genes was evaluated using western blotting and real-time PCR.Results: Harmine increased the survival rate of DPSCs in a time--dependent manner, but higher doses (above 80 μM) had a toxic effect. On day 14, Alizarin Red staining showed increased differentiation of odontoblasts in the harmine-treated groups compared to the untreated groups. Furthermore, harmine increased the expression of Runx2, DSPP, and DMP1 genes and proteins.Conclusion: These findings suggest that harmine has a significant impact on the differentiation and proliferation of odontoblasts in DPSCs, likely due to its various properties and role in healing various diseases. Therefore, harmine could serve as a potential therapeutic agent for promoting dental tissue regeneration using DPSCs.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-12"},"PeriodicalIF":1.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memory of an exquisite medicinal chemist, Prof. Morris Robins. 纪念杰出的药物化学家莫里斯-罗宾斯教授。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-07 DOI: 10.1080/15257770.2024.2426160

Erik De Clercq

引用次数: 0

The effect of VEGF and KDR gene variants on Crimean-Congo hemorrhagic fever. 血管内皮生长因子和 KDR 基因变异对克里米亚-刚果出血热的影响。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-11-04 DOI: 10.1080/15257770.2024.2423887

Laleh Bahrikarehmi, Nilufer Kuruca, Umut Safiye Say Coskun, Ayse Feyda Nursal, Harun Albayrak, Serbulent Yigit

{"title":"The effect of VEGF and KDR gene variants on Crimean-Congo hemorrhagic fever.","authors":"Laleh Bahrikarehmi, Nilufer Kuruca, Umut Safiye Say Coskun, Ayse Feyda Nursal, Harun Albayrak, Serbulent Yigit","doi":"10.1080/15257770.2024.2423887","DOIUrl":"https://doi.org/10.1080/15257770.2024.2423887","url":null,"abstract":"Background: Crimean-Congo hemorrhagic fever (CCHF), an acute viral hemorrhagic fever disease, has a high mortality rate among humans. Hemorrhagic propensity is caused by coagulation malfunction and increased capillary permeability brought on by the resultant vascular injury. Vascular endothelial growth factor (VEGF) and VEGF receptor-2, or KDR (kinase insert domain containing receptor), are effective in vasculogenesis and angiogenesis. CCHF was stated to have endothelial dysfunction. This study aimed to evaluate whether the VEGF and KDR gene variants contribute to the development of CCHF in the Turkish population.Methods: A total of 101 subjects, including 51 CCHF patients and 50 healthy controls, were included in the study. The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used to genotype VEGF 936 C > T (rs3025039) and KDR - 604 T > C (rs2071559) variants. The results were statistically analyzed.Results: The VEGF 936 C > T genotype and allele distributions did differ significantly between the patients and the controls. The subjects carrying the C/C genotype and C allele had a higher risk of developing CCHF than the control group (p˂0.05). There was a statistically significant association between the controls and the patients in terms of VEGF 936 C > T C/C versus C/T + T/T (p˂0.05, OR:3.273, 95%Cl: 1.44-7.63). The KDR - 604 T > C variant's allele and genotype distribution were not significantly different between the patients and controls.Conclusion: This study suggests the VEGF 936 C > T variant is a genetic marker of sensitivity to CCHF among the Turkish population and may help protect against the disease.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-9"},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0