Nucleosides, Nucleotides & Nucleic Acids最新文献_第9页

Identification of lung adenocarcinoma subtypes based on mitochondrial energy metabolism-related genes. 基于线粒体能量代谢相关基因的肺腺癌亚型鉴定。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-06-26 DOI: 10.1080/15257770.2024.2369093

Jianyang Ding, Keng Chen, Xuhui Wu

{"title":"Identification of lung adenocarcinoma subtypes based on mitochondrial energy metabolism-related genes.","authors":"Jianyang Ding, Keng Chen, Xuhui Wu","doi":"10.1080/15257770.2024.2369093","DOIUrl":"https://doi.org/10.1080/15257770.2024.2369093","url":null,"abstract":"Background: Identifying subtypes of lung adenocarcinoma (LUAD) patients based on mitochondrial energy metabolism and immunotherapy sensitivity is essential for precision cancer treatment.Methods: LUAD subtypes were identified using unsupervised consensus clustering, and results were subjected to immune and tumor mutation analyses. DEGs between subtypes were identified by differential analysis. Functional enrichment and PPI network analyses were conducted. Patients were classified into high and low expression groups based on the expression of the top 10 hub genes, and survival analysis was performed. Drugs sensitive to feature genes were screened based on the correlation between hub gene expression and drug IC50 value. qRT-PCR and western blot were used for gene expression detection, and CCK-8 and flow cytometry were for cell viability and apoptosis analysis.Results: Cluster-1 had significantly higher overall survival and a higher degree of immunoinfiltration and immunophenotypic score, but a lower TIDE score, DEPTH score, and TMB. Enrichment analysis showed that pathways and functions of DEGs between two clusters were mainly related to the interaction of receptor ligands with intracellular proteases. High expression of hub genes corresponded to lower patient survival rates. The predicted drugs with high sensitivity to feature genes were CDK1: Ribavirin (0.476), CCNB2: Hydroxyurea (0.474), Chelerythrine (0.470), and KIF11: Ribavirin (0.471). KIF11 and CCNB2 were highly expressed in LUAD cells and promoted cell viability and inhibited cell apoptosis.Conclusion: This study identified two subtypes of LUAD, with cluster-1 being more suitable for immunotherapy. These results provided a reference for the development of precision immunotherapy for LUAD patients.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.1,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved synthesis of guanosine TNA phosphoramidite for oligonucleotide synthesis. 用于寡核苷酸合成的鸟苷 TNA 亚磷酰胺的改进合成法。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-06-21 DOI: 10.1080/15257770.2024.2369688

Biju Majumdar, Daisy Sarma, Erica M Lee, Noah A Setterholm, John C Chaput

引用次数: 0

Investigation of bisindole-linked pyrimidine moieties: synthesis using strantium-aluminum supported strontium aluminate nanophosphors catalyst, DNA reactivity, and in silico molecular docking studies. 双吲哚连接嘧啶分子的研究：使用铝酸锶纳米磷酸盐催化剂合成、DNA 反应性和分子对接研究。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-30 DOI: 10.1080/15257770.2024.2358901

Hanumesh, M K Amshumali, P Prachi, K Yogendra, N Madhusudhana, B Vinay Kumar

{"title":"Investigation of bisindole-linked pyrimidine moieties: synthesis using strantium-aluminum supported strontium aluminate nanophosphors catalyst, DNA reactivity, and in silico molecular docking studies.","authors":"Hanumesh, M K Amshumali, P Prachi, K Yogendra, N Madhusudhana, B Vinay Kumar","doi":"10.1080/15257770.2024.2358901","DOIUrl":"https://doi.org/10.1080/15257770.2024.2358901","url":null,"abstract":"In this communication, an innovative and straightforward protocol for the one-pot catalytic synthesis of bis(indolyl)pyrimidine derivatives and their DNA binding abilities is presented. The synthesis involves the condensation of indole with diverse substituted pyrimidine-5-carbaldehydes, employing cost-effective and reusable Sr-Al supported nanophosphors, specifically strontium aluminate (SrAl2O4), as a catalyst. In particular, this method does not require the use of toxic solvents. The Sr-Al supported nanophosphorus catalyst exhibited sustained activity over multiple cycles and showed no significant decline while maintaining its strictly heterogeneous properties. The bis(indolyl)pyrimidine derivatives were extensively characterized using spectroscopic and analytical techniques. Furthermore, the interaction between these derivatives and CT-DNA was investigated by absorption spectroscopy, viscosity measurement, and in silico molecular docking studies. Photoinduced cleavage studies demonstrated the photonuclease activity of the compound against pUC19 DNA upon exposure to UV-visible radiation.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of CSNK1D and KLK6 as two common upregulated genes present in BRCA1 mutated triple-negative breast cancer and ovarian epithelial carcinoma. 确定 CSNK1D 和 KLK6 是 BRCA1 突变三阴性乳腺癌和卵巢上皮癌中两个常见的上调基因。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-23 DOI: 10.1080/15257770.2024.2357267

Fatima Lakis, Rita Ayoub, Wissam H Faour, Mohammad Makki, Hanane Yassine, Hussein Fayyad-Kazan, Fadi Abdel Sater

{"title":"Identification of CSNK1D and KLK6 as two common upregulated genes present in BRCA1 mutated triple-negative breast cancer and ovarian epithelial carcinoma.","authors":"Fatima Lakis, Rita Ayoub, Wissam H Faour, Mohammad Makki, Hanane Yassine, Hussein Fayyad-Kazan, Fadi Abdel Sater","doi":"10.1080/15257770.2024.2357267","DOIUrl":"https://doi.org/10.1080/15257770.2024.2357267","url":null,"abstract":"Deficiency in the breast cancer type 1 (BRCA1) gene expression predisposes to triple-negative breast cancer (TNBC) and ovarian cancer (OC). We previously identified by Comparative Genomic Hybridization (CGH) array a gain in the 17q25.3 genomic region in 90% of the BRCA1 mutated TNBC tissues, where 17 genes were up-regulated. A second region (Chr19_45681759_54221324) was identified as the second most frequent gain in the BRCA1-mutated population and has not yet been described in the context of BRCA1 mutation. We thus aimed to validate the expression of the Casein kinase 1 delta (CSNK1D) gene of Chr17 in TNBC and OC cell lines and to investigate the expression of genes of Chr19 in TNBC cell lines and tissues as well as in OC cell lines. Expression level of the genes of the 17q25.3, 19q13.32,13.33 and 13.41 chromosomal regions was analyzed using RT-PCR in BRCA1 deficient TNBC and OC cell lines, as well as in 10 BRCA1-mutated TNBC tissues versus 10 wild type carriers. Our results revealed a significant upregulation of CSNK1D gene expression in BRCA1 deficient TNBC and OC cell lines when compared to control ones, and a significant aberration in the expression of the other six genes of Chr19 was observed. Interestingly, upregulation of kallikrein-related peptidase 6 (KLK6) was detected among the BRCA1 deficient TNBC (cell lines and tissues) and OC cell lines. In conclusion, our results suggested that CSNK1D and KLK6 expression levels could be very promising in the search for biomarkers for BRCA1 deficient TNBC and OC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of rs2236242 at SERPINA12 gene and vaspin level on papillary thyroid carcinoma. SERPINA12基因中的rs2236242和vaspin水平对甲状腺乳头状癌的影响

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-23 DOI: 10.1080/15257770.2024.2354427

Sahar Torki, Masoumeh Nezhadali, Mehdi Hedayati, Helma Karimi, S Adeleh Razavi, Leila Najd Hassan Bonab

{"title":"The role of rs2236242 at SERPINA12 gene and vaspin level on papillary thyroid carcinoma.","authors":"Sahar Torki, Masoumeh Nezhadali, Mehdi Hedayati, Helma Karimi, S Adeleh Razavi, Leila Najd Hassan Bonab","doi":"10.1080/15257770.2024.2354427","DOIUrl":"10.1080/15257770.2024.2354427","url":null,"abstract":"Several studies showed that adipokines are associated with types of cancer which are documented to be effective in cancer biology. This study aimed to determine the relationship between vaspin rs2236242 polymorphism and the vaspin level with papillary thyroid carcinoma (PTC), and multinodular goiter (MNG). In this cross-sectional study, we recruited 170 candidates. Ninety patients with newly diagnosed (PTC 60 patients and MNG 30 patients), and 80 participants as a control group referred to Shariati Hospital, Tehran, Iran, were enrolled in the study. The vaspin hormone measurements were conducted utilizing the Elisa Kit. Using Tetra amplification resistant-mutation system polymerase chain reaction (T-ARMS-PCR), the genotype of single nucleotide polymorphism (SNP) rs2236242 was determined. The statistical analysis was performed using SPSS software version 20. Our findings showed significant age and genotype frequency differences in three groups (p-value < 0.05). There was no significant difference in vaspin levels between PTC, and control groups. The level of vaspin in MNG compared to the control group had significantly different, but there were no differences after adjustment for age. Results showed the genotypes of vaspin rs2236242 polymorphism are not associated with the level of vaspin. The genotypes and allele frequencies of vaspin rs2236242 in the PTC and MNG groups were significant compared to the control group. We have found vaspin rs2236242 gene polymorphism as a potential marker of papillary thyroid cancer. The A allele of the vaspin SNP rs2236242 plays a protective role against PTC and MNG. SNP at rs2236242 was not significantly associated with vaspin levels.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-12"},"PeriodicalIF":1.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEG3 rs7158663 genetic polymorphism is associated with the risk of hepatocellular carcinoma. MEG3 rs7158663 基因多态性与肝细胞癌风险有关。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-10 DOI: 10.1080/15257770.2024.2350513

Yong Wang, Fulei Gao, Jian Lu

{"title":"MEG3 rs7158663 genetic polymorphism is associated with the risk of hepatocellular carcinoma.","authors":"Yong Wang, Fulei Gao, Jian Lu","doi":"10.1080/15257770.2024.2350513","DOIUrl":"https://doi.org/10.1080/15257770.2024.2350513","url":null,"abstract":"Recently, a meta-analysis has shown that a potentially functional genetic polymorphism (rs7158663 A > G) on the cancer-associated lncRNA MEG3 is associated with the risk of many types of cancer. Given the important role of MEG3 in the development of hepatocellular carcinoma (HCC), the current study evaluated the association of the rs7158663 genetic polymorphism with HCC risk. A total of 271 HCC patients and 267 healthy individuals were included in the current case-control study. Direct sequencing was used to detect the rs7158663 locus genotype of the included individuals. The case-control study showed that the MEG3 rs7158663 genetic polymorphism was associated with the increased risk of developing HCC [GA vs. GG: OR = 1.63, 95% CI = 1.14-2.34, p = 0.009; AA vs. GG: OR = 2.10, 95% CI = 1.10-4.08, p = 0.03; (GA + AA) vs. GG: OR = 1.70, 95% CI = 1.21-2.40, p = 0.003; A vs. G: OR = 1.53, 95% CI = 1.17-2.00, p = 0.002]. In addition, the genotype-tissue expression showed that the rs7158663 AA or GA genotype was associated with reduced MEG3 expression. Bioinformatic analysis showed that the rs7158663 genetic polymorphism not only affects the binding of transcription factors but also interacts with multiple genes through chromatin loops. In summary, the current findings suggest that the rs7158663 genetic polymorphism affecting MEG3 expression is associated with HCC risk and may serve as a marker of genetic susceptibility to HCC. However, the specific molecular mechanisms of the rs7158663 genetic polymorphism in the development of HCC need to be further revealed.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.3,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140904739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, conformational analysis and antimicrobial activity of 10-benzyl-1,2,4-triazolo[4,3-b]1,2,4-triazino[5,6-b]indole acyclo C-nucleoside analogs. 10-苄基-1,2,4-三唑并[4,3-b]1,2,4-三嗪并[5,6-b]吲哚酰环 C 核苷类似物的合成、构象分析和抗菌活性。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-02 DOI: 10.1080/15257770.2024.2348741

Adel Z Nasr, Reda M Keshk, El-Sayed M Abdelrehim, Asmaa S Sallam

引用次数: 0

Nucleic acids based integrated macromolecular complexes for SiRNA delivery: Recent advancements. 用于 SiRNA 递送的基于核酸的集成大分子复合物：最新进展。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-05-01 DOI: 10.1080/15257770.2024.2347499

Dilpreet Singh, Lovedeep Singh, Simranjeet Kaur, Akshita Arora

{"title":"Nucleic acids based integrated macromolecular complexes for SiRNA delivery: Recent advancements.","authors":"Dilpreet Singh, Lovedeep Singh, Simranjeet Kaur, Akshita Arora","doi":"10.1080/15257770.2024.2347499","DOIUrl":"https://doi.org/10.1080/15257770.2024.2347499","url":null,"abstract":"The therapeutic potential of small interfering RNA (siRNA) is monumental, offering a pathway to silence disease-causing genes with precision. However, the delivery of siRNA to target cells in-vivo remains a formidable challenge, owing to degradation by nucleases, poor cellular uptake and immunogenicity. This overview examines recent advancements in the design and application of nucleic acid-based integrated macromolecular complexes for the efficient delivery of siRNA. We dissect the innovative delivery vectors developed in recent years, including lipid-based nanoparticles, polymeric carriers, dendrimer complexes and hybrid systems that incorporate stimuli-responsive elements for targeted and controlled release. Advancements in bioconjugation techniques, active targeting strategies and nanotechnology-enabled delivery platforms are evaluated for their contribution to enhancing siRNA delivery. It also addresses the complex interplay between delivery system design and biological barriers, highlighting the dynamic progress and remaining hurdles in translating siRNA therapies from bench to bedside. By offering a comprehensive overview of current strategies and emerging technologies, we underscore the future directions and potential impact of siRNA delivery systems in personalized medicine.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-24"},"PeriodicalIF":1.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of nucleoside analogues, lipophilic prodrugs and elaidic acids on core signaling pathways in cancer cells 核苷类似物、亲脂原药和鞣酸对癌细胞核心信号通路的影响

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-04-15 DOI: 10.1080/15257770.2024.2339952

Marika A. Frańczak, Claudine van der Sande, Elisa Giovannetti, Godefridus J. Peters

引用次数: 0

Promoter of lncRNA MORT is aberrantly methylated in colorectal cancer lncRNA MORT 的启动子在结直肠癌中异常甲基化

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-04-15 DOI: 10.1080/15257770.2024.2328732

Aylar Nazari, Tayyebeh Ghasemi, Mohammad Khalaj-Kondori, Ramin Fathi

引用次数: 0