LncRNA GABPB1-AS1 is a potential target for the diagnosis of prostate cancer.

IF 1.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-01 Epub Date: 2024-07-14 DOI:10.1080/15257770.2024.2372315

Qi Chen, Yongsheng Pan, Xiufeng Yang, Hua Zhu, Bing Zheng, Longtao Ju

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引用次数: 0

Abstract

Background: Prostate cancer is an adverse tumor that occurs in the male reproductive system. The symptoms of patients in the early stage are not obvious and are generally difficult to detect.

Aim: The aim of this study was to determine the regulation of lncRNA GABPB1-AS1 (GABPB1-AS1) on prostate cancer progression and explore the diagnostic potential of GABPB1-AS1.

Methods: The contents of serum GABPB1-AS1 and miR-330-3p were examined by RT-qPCR assay. The functions of silencing GABPB1-AS1 and miR-330-3p inhibitor in prostate cancer cells were determined using transfection assay, CCK-8 assay and Transwell assay. The target of GABPB1-AS1 was predicted and verified at the molecular level by bioinformatics and luciferase reporter gene assay. The function of GABPB1-AS1 in prostate cancer diagnosis was evaluated via ROC method.

Results: GABPB1-AS1 was upregulated in prostate cancer serum, which was associated with patients' Gleason score and TNM stage. Mechanistically, GABPB1-AS1 directly targeted miR-330-3p, and there was a negative correlation between them. Reduced levels of GABPB1-AS1 in cells after knockdown of GABPB1-AS1 resulted in decreased prostate cancer cell growth and activity, and these inhibitory effects were repaired by miR-330-3p inhibitor.

Conclusion: The present study confirmed that GABPB1-AS1 was overexpressed in prostate cancer, and its sponge miR-330-3p may be an effective target for timely diagnosis of prostate cancer.

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LncRNA GABPB1-AS1 是诊断前列腺癌的潜在靶点。

背景：前列腺癌是一种发生在男性生殖系统的恶性肿瘤。目的：本研究旨在确定lncRNA GABPB1-AS1（GABPB1-AS1）对前列腺癌进展的调控作用，并探索GABPB1-AS1的诊断潜力：方法：通过RT-qPCR检测血清中GABPB1-AS1和miR-330-3p的含量。采用转染试验、CCK-8 试验和 Transwell 试验测定沉默 GABPB1-AS1 和 miR-330-3p 抑制剂在前列腺癌细胞中的功能。通过生物信息学和荧光素酶报告基因检测，在分子水平上预测并验证了 GABPB1-AS1 的靶点。通过ROC法评估了GABPB1-AS1在前列腺癌诊断中的功能：结果：GABPB1-AS1在前列腺癌血清中上调，与患者的Gleason评分和TNM分期相关。从机理上讲，GABPB1-AS1 直接靶向 miR-330-3p，两者之间存在负相关。GABPB1-AS1被敲除后，细胞中GABPB1-AS1水平降低，导致前列腺癌细胞生长和活性下降，而这些抑制作用可被miR-330-3p抑制剂修复：本研究证实，GABPB1-AS1在前列腺癌中过表达，其海绵体miR-330-3p可能是及时诊断前列腺癌的有效靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.