Neuroscience Letters最新文献_第4页

MiR-222-3p regulates methamphetamine-induced behavioral sensitization through PP2A–AKT signaling pathway in the dorsal striatum of male mice MiR-222-3p通过PP2A-AKT信号通路调节雄性小鼠背侧纹状体中甲基苯丙胺诱导的行为致敏。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-03-01 DOI: 10.1016/j.neulet.2025.138181

Gang Chen , Xingyao Chen , Wei Han , Baoyao Gao , Min Liang , Tao Li , Xinshe Liu

{"title":"MiR-222-3p regulates methamphetamine-induced behavioral sensitization through PP2A–AKT signaling pathway in the dorsal striatum of male mice","authors":"Gang Chen , Xingyao Chen , Wei Han , Baoyao Gao , Min Liang , Tao Li , Xinshe Liu","doi":"10.1016/j.neulet.2025.138181","DOIUrl":"10.1016/j.neulet.2025.138181","url":null,"abstract":"<div><div>Drug addiction is a chronic and recurrent brain disease. Our previous research demonstrated that the B subunit of phosphatase 2A (PP2A/B) increased in the dorsal striatum (DS) of methamphetamine (METH)-sensitized mice. Interestingly, studies indicate that PP2A/B can also interplay with microRNA (miRNA). In this study, we investigated seven miRNAs that have been reported to potentially interplay with PP2A/B, and our results showed that miR-222-3p significantly decreased in the DS of METH-sensitized mice. We further used dual-luciferase reporter assay to clarify the regulatory relationship between miR-222-3p and PP2A/B mRNA, and we also constructed adeno-associated virus (AAV) to overexpress miR-222-3p in the DS to further examine the influence of miR-222-3p on METH-induced behavioral sensitization. Our results demonstrated that miR-222-3p did interplay with PP2A/B mRNA and overexpressed miR-222-3p could significant attenuate METH-induced behavioral sensitization. At the same time, overexpressed miR-222-3p could reverse the change in the PP2A–AKT signaling pathway in the DS of METH-sensitized mice. Our results indicate that overexpression of miR-222-3p in the DS might attenuate METH-induced behavioral sensitization through the PP2A–AKT signaling pathway.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138181"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of pentosidine accumulation in stress-induced social behavioral deficits 戊苷积累在应激诱发的社会行为缺陷中的作用。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-27 DOI: 10.1016/j.neulet.2025.138180

Mayuko Masada , Kazuya Toriumi , Kazuhiro Suzuki , Mitsuhiro Miyashita , Masanari Itokawa , Makoto Arai

{"title":"Role of pentosidine accumulation in stress-induced social behavioral deficits","authors":"Mayuko Masada , Kazuya Toriumi , Kazuhiro Suzuki , Mitsuhiro Miyashita , Masanari Itokawa , Makoto Arai","doi":"10.1016/j.neulet.2025.138180","DOIUrl":"10.1016/j.neulet.2025.138180","url":null,"abstract":"<div><div>The mechanisms underlying schizophrenia, a psychiatric disorder characterized by significant social and behavioral impairments, remain poorly understood. However, glycation stress, driven by the accumulation of advanced glycation end products (AGEs) such as pentosidine, has been implicated in its pathogenesis. Therefore, this study aimed to explore the role of pentosidine in stress-induced social behavioral deficits using a mouse model of social defeat stress (SDS). Mice exposed to SDS displayed individual differences in sociability, and were categorized into stress-susceptible and stress-resilient phenotypes based on their social interaction ratio. Pentosidine levels were significantly elevated in the plasma and the prefrontal cortex (Pfc) of the susceptible group, which correlated with increased social avoidance and decreased interaction times. Administration of pyridoxamine, an AGE synthesis inhibitor, during SDS exposure mitigated these behavioral deficits, and suppressed pentosidine accumulation in both the plasma and Pfc. These findings provide the first evidence linking pentosidine accumulation to stress susceptibility, indicating the involvement of a molecular pathway through which glycation stress influences social behavior. Future studies should further elucidate the mechanisms underlying the effects of pentosidine on behavior, and explore its broader implications in psychiatric disorders.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138180"},"PeriodicalIF":2.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel prefrontal cortex and hippocampus combined brain slice based on in vivo diffusion tensor imaging of healthy male rats 基于体内弥散张量成像的健康雄性大鼠前额皮质和海马联合脑切片

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-24 DOI: 10.1016/j.neulet.2025.138171

Lijun Zhang , Qian Zhou , Guanghao Zhang , Changzhe Wu , Wei Rong , Shiji He , Xiaolin Huo , Cheng Zhang

{"title":"A novel prefrontal cortex and hippocampus combined brain slice based on in vivo diffusion tensor imaging of healthy male rats","authors":"Lijun Zhang , Qian Zhou , Guanghao Zhang , Changzhe Wu , Wei Rong , Shiji He , Xiaolin Huo , Cheng Zhang","doi":"10.1016/j.neulet.2025.138171","DOIUrl":"10.1016/j.neulet.2025.138171","url":null,"abstract":"<div><div>The pathway between the prefrontal cortex (PFC) and hippocampus (HPC) has been associated with various psychiatric disorders. While hippocampal brain slices are extensively utilized, their use has traditionally been constrained in studying long connectivity between PFC and HPC due to nerve fiber rupture during the slicing process. Consequently, optimizing brain slice preparation is crucial. The experiment consisted of three phases. Initially, the structural connection of the PFC-HPC pathway was examined using diffusion tensor imaging (DTI) data from healthy male rats. Subsequently, combined PFC-HPC brain slices were created through vibratome based on imaging acquisition. Finally, the morphology and electrophysiology of the combined brain slices were analyzed. DTI findings revealed numerous nerve fibers linking the two brain regions in the rat brain. Subsequently, a successful preparation of combined PFC-HPC brain slices cut at a 7 – 8° angle relative to the middle sagittal plane was achieved using a vibratome. Hematoxylin and eosin staining results confirmed that PFC-HPC fibers remained well-preserved in the combined brain slice. Electrophysiological recordings indicated that synchronized neuronal activity occurred in the HPC upon PFC stimulation, which depended on hippocampal activity and the integrity of PFC-to-HPC connectivity. A novel procedure for the successful preparation of healthy combined HPC-PFC brain slices, maintaining a complete fiber bundle connection between PFC and HPC, is proposed. This methodology enhances the understanding of the preservation of PFC-HPC connectivity in specific angled brain slice preparations, thereby facilitating neuroscience research focused on the longrange circuitry of subregions of interest.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138171"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture inhibits oxidative stress and improves cognitive function by downregulating the Ang II/AT1R/NOX axis in chronic cerebral ischemia rats 电针通过下调慢性脑缺血大鼠Ang II/AT1R/NOX轴抑制氧化应激，改善认知功能。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-23 DOI: 10.1016/j.neulet.2025.138179

Qi Ai , Jurui Wei , Bijun Luo , Zixuan Wang , Jun Dong , Jiumei Zhao , Wenqing Xu , Lin Chen , Wenyao Fang , Songjie Zhu

{"title":"Electroacupuncture inhibits oxidative stress and improves cognitive function by downregulating the Ang II/AT1R/NOX axis in chronic cerebral ischemia rats","authors":"Qi Ai , Jurui Wei , Bijun Luo , Zixuan Wang , Jun Dong , Jiumei Zhao , Wenqing Xu , Lin Chen , Wenyao Fang , Songjie Zhu","doi":"10.1016/j.neulet.2025.138179","DOIUrl":"10.1016/j.neulet.2025.138179","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the effects of Electroacupuncture (EA) on chronic cerebral ischemia (CCI).</div></div><div><h3>Methods</h3><div>A CCI rat model was established by blocking the common carotid arteries. Model rats were treated with EA at the “Baihui” (GV20) and “Dazhui” (GV14) acupoints and/or ARB. Ang II and AT1R expression in the artery and hippocampal tissues was determined. Immunohistochemistry staining was used to detect RECA-1 expression in hippocampal tissues. NOX2, NOX4, SOD1, SOD2, ROS, and MDA levels were examined. Morris water maze and TUNEL staining were used to explore the effects of EA on cognitive impairment and apoptosis, respectively.</div></div><div><h3>Results</h3><div>Ang II and AT1R levels were reduced by EA in CCI rats. RECA-1 expression was reduced in model rats, while EA and ARB increased its expression. EA inhibited oxidative stress in CCI rats. Besides, EA improved cognitive impairment in CCI rats. Apoptosis in the hippocampal tissues of CCI rats was inhibited by EA treatment. Furthermore, inhibition of Ang II/AT1R/NOX axis promoted the therapeutic effects of EA on oxidative stress and cognitive impairment in model rats.</div></div><div><h3>Conclusions</h3><div>EA treatment at the “Baihui” (GV20) and “Dazhui” (GV14) acupoints is effective against CCI-induced cognitive impairment and oxidative stress by downregulating Ang II/AT1R/NOX axis.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138179"},"PeriodicalIF":2.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotinamide adenine dinucleotide alleviates neuroinflammation in rats with traumatic brain injury 烟酰胺腺嘌呤二核苷酸减轻创伤性脑损伤大鼠神经炎症。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-22 DOI: 10.1016/j.neulet.2025.138178

Huancheng Wu , Mengli Jin , Jiandong Hu , Fenge Li

{"title":"Nicotinamide adenine dinucleotide alleviates neuroinflammation in rats with traumatic brain injury","authors":"Huancheng Wu , Mengli Jin , Jiandong Hu , Fenge Li","doi":"10.1016/j.neulet.2025.138178","DOIUrl":"10.1016/j.neulet.2025.138178","url":null,"abstract":"<div><h3>Objective</h3><div>To characterize the pathology and pathophysiological processes within 6 h after Traumatic brain injury (TBI) in rats, elucidate the neuroprotective effects and the underlying mechanisms of Nicotinamide Adenine Dinucleotide (NAD) in the early stage of TBI to explore the feasibility and clinical benefits of applying NAD directly to the localized injury after TBI.</div></div><div><h3>Material and Methods</h3><div>54 male Sprague-Dawley (SD) rats aged 6–8 weeks were randomly assigned equally to three groups, sham-operated surgery (SO) with saline treatment (SO + Saline), TBI with saline treatment (TBI + Saline), and TBI with 10 μM NAD treatment (TBI + NAD). The whole brain tissues were collected at 1, 3, and 6 h following the procedure. Levels of biomarkers for TBI including S100β, TNF-α, occludin, PPARβ/δ were measured.</div></div><div><h3>Results</h3><div>Significant neuroinflammation was observed in the rat brains after TBI, which peaked at 3 h following injury. Significant changes in S100β, TNF-α, PPARβ/δ, and occluding were also observed. Treatment with NAD significantly alleviated neuroinflammation at 1 h following TBI.</div></div><div><h3>Conclusions</h3><div>TBI caused severe neuroinflammation in rat brains, which peaked at 3 h following injury. Treatment with NAD alleviated neuroinflammation in TBI rats.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138178"},"PeriodicalIF":2.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the antidepressant effects of amantadine combined with amitriptyline in a chronic unpredictable mild stress model in mice 研究金刚烷胺联合阿米替林在小鼠慢性不可预测轻度应激模型中的抗抑郁作用

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138169

Vijay Kumar, Gaurav Doshi

{"title":"Investigating the antidepressant effects of amantadine combined with amitriptyline in a chronic unpredictable mild stress model in mice","authors":"Vijay Kumar, Gaurav Doshi","doi":"10.1016/j.neulet.2025.138169","DOIUrl":"10.1016/j.neulet.2025.138169","url":null,"abstract":"<div><div>Amantadine, an N-Methyl-D-Aspartate (NMDA) receptor antagonist traditionally used as an antiviral drug, was investigated for its potential antidepressant effect in combination with amitriptyline, a well-established tricyclic antidepressant, in a Chronic Unpredictable Mild Stress (CUMS) animal model of depression. This study aimed to evaluate the efficacy of this drug combination in alleviating depression-like symptoms. Behavioral assessments were conducted using the Forced Swim Test, Tail Suspension Test, Actophotometer test, and Sucrose Preference Test to measure depressive effect. Further biochemical analyses revealed a marked reduction in Brain-derived neurotrophic factor (BDNF) and norepinephrine (NE) levels in the CUMS group compared with the control group. BDNF is crucial for neuroplasticity, synaptic regulation, and neuronal survival and its reduced level is linked to the development of depression. Similarly, NE, a key neurotransmitter involved in mood regulation, is often decreased in depressive states. Conversely, the CUMS group exhibited a significant increase in Tumour necrosis factor-alpha (TNF-α) levels, indicating enhanced inflammatory response, which is also associated with depression. Treatment with the combination of amantadine and amitriptyline resulted in a significant antidepressant-like effect, as demonstrated by improved behavioral parameters and normalization of this biomarker the increase in BDNF and NE levels along with a reduction in TNF-α.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138169"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased anxiety-like behavior and trigeminal ganglion BDNF levels persist in rats with temporomandibular joint arthritis even after resolution of the nociceptive process 颞下颌关节关节炎大鼠的焦虑样行为和三叉神经节BDNF水平持续下降，即使在伤害过程解决后。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138166

Cristina Balensiefer Vicenzi , Dirson João Stein , Josimar Macedo de Castro , Beatriz Lima Silveira , Alanis da Silva Melo , Etiane Micheli Meyer Callai , Fernanda Visioli , Wolnei Caumo , Alexandre Silva de Quevedo , John K. Neubert , Iraci L.S. Torres

{"title":"Decreased anxiety-like behavior and trigeminal ganglion BDNF levels persist in rats with temporomandibular joint arthritis even after resolution of the nociceptive process","authors":"Cristina Balensiefer Vicenzi , Dirson João Stein , Josimar Macedo de Castro , Beatriz Lima Silveira , Alanis da Silva Melo , Etiane Micheli Meyer Callai , Fernanda Visioli , Wolnei Caumo , Alexandre Silva de Quevedo , John K. Neubert , Iraci L.S. Torres","doi":"10.1016/j.neulet.2025.138166","DOIUrl":"10.1016/j.neulet.2025.138166","url":null,"abstract":"<div><div>In this study, pain- and anxiety-like behaviors, locomotor and exploratory activity, histological and biomarker parameters were evaluated following induction of a temporomandibular joint (TMJ) arthritis model in rats. Twenty-two adult male Wistar rats were assigned to receive either saline (sham group) or Complete Freund’s Adjuvant (CFA − pain group) into the right TMJ. Mechanical allodynia was assessed using the facial electronic von Frey (VF) test, while thermal hyperalgesia was assessed using the Orofacial Pain Assessment Device (OPAD) assay. Open-field and plus-maze tests were used to assess locomotor and exploratory activity and anxiety-like behaviors, respectively. BDNF, IL-1β, and IL-10 levels were analyzed by ELISA in both the ipsilateral and contralateral trigeminal ganglion (TG). The tissues adjacent to the TMJ were histologically evaluated for the inflammatory process. According to distribution, data were analyzed by GEE, independent <em>t</em>-test, or Mann-Whitney test. Significance was set at P < 0.05. On the ninth day following CFA injection, pain-rats presented mechanical allodynia, which persisted until the twenty-first day, a decrease in the number of OPAD licks, decreased BDNF levels in the contralateral TG, and an increase in the ipsilateral TG BDNF and IL-1β levels, with inflammatory infiltrates in the tissues adjacent to the TMJ (27 days). TMJ arthritis also resulted in a reduction of the anxiety index (AI) after 26 days. This study reveals that this model is effective for examining chronic alterations related to TMJ arthritis and for identifying new anti-inflammatory drugs for pain management.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138166"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using MRI to Determine Drug Concentration in Convection-Enhanced Delivery: A Proof-of-Concept study 使用MRI来确定对流增强输送中的药物浓度：一项概念验证研究。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138170

Xiaojie Ding , Ming Qi , Yuan Zhou , Ying Qi , Di Chen , Xinyu Yang , Chunxia Ji , Yu Yao

{"title":"Using MRI to Determine Drug Concentration in Convection-Enhanced Delivery: A Proof-of-Concept study","authors":"Xiaojie Ding , Ming Qi , Yuan Zhou , Ying Qi , Di Chen , Xinyu Yang , Chunxia Ji , Yu Yao","doi":"10.1016/j.neulet.2025.138170","DOIUrl":"10.1016/j.neulet.2025.138170","url":null,"abstract":"<div><div>Convection-enhanced delivery (CED) bypasses the blood–brain barrier and avoids systemic exposure to the drug. However, systemic pharmacokinetic characteristics of a drug cannot be applied when delivered via CED. This study aims to provide a first proof-of-concept framework for noninvasively evaluating pharmacokinetics in CED. We investigated local concentration and the distribution of a gadolinium-based contrast agent in rat brains using magnetic resonance imaging (MRI). Standards of gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) were scanned on a 7.0 T MRI system in rat brain tissue suspension. <em>T</em><sub>1</sub> values were mapped and <em>T</em><sub>1</sub> relaxivity of Gd-DTPA was calculated. Subsequently, evaluation in live animals was performed by infusing Gd-DTPA into the rat striatum followed by scans for <em>T</em><sub>1</sub> mapping. The quantitative relationship between imaging data and Gd-DTPA concentration deduced from standard scans was used to determine the voxel-level concentration of Gd-DTPA in rat brains. Concentration maps were constructed from voxel-level concentration data. The Gd-DTPA concentration in tissue suspension and 1/ <em>T</em><sub>1</sub> showed a linear relationship with <em>R</em><sup>2</sup> of 0.9919 (p < 0.0001). The <em>T</em><sub>1</sub> relaxivity of Gd-DTPA at the experimental condition was 4.199 mM<sup>−1</sup> s<sup>−1</sup>. Within the rat brain parenchyma, the mean volume of distribution to initial volume ratio (Vd/Vi) of Gd-DTPA was calculated to be 6.02. Notably, the infusion center’s concentration exhibited a decreasing pattern, while the peripheral region’s concentration remained relatively stable over the observed duration. This study showed the spatial distribution of Gd-DTPA concentration and its temporal change, suggesting that using MRI to determine the Gd-DTPA concentration is feasible with good accuracy and data quality.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138170"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of the TRPM2 cation channel attenuates morphine tolerance by modulating endoplasmic reticulum stress and apoptosis in rats 抑制TRPM2阳离子通道通过调节内质网应激和细胞凋亡减弱吗啡耐受性。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-18 DOI: 10.1016/j.neulet.2025.138168

Arzuhan Cetindag Ciltas , Ercan Ozdemir , Handan Gunes , Aysegul Ozturk

{"title":"Inhibition of the TRPM2 cation channel attenuates morphine tolerance by modulating endoplasmic reticulum stress and apoptosis in rats","authors":"Arzuhan Cetindag Ciltas , Ercan Ozdemir , Handan Gunes , Aysegul Ozturk","doi":"10.1016/j.neulet.2025.138168","DOIUrl":"10.1016/j.neulet.2025.138168","url":null,"abstract":"<div><div>Opioid drugs such as morphine are frequently preferred drugs for severe pain in cancer and chronic diseases, but long-term use causes opioid tolerance. The mechanism of tolerance to opioids is quite complex and not fully understood. Our aim in this study was to investigate the effects of TRPM2 cation channel antagonists N-(p-amylcinnamoyl) anthranilic acid (ACA) and 2-aminoethoxydiphenyl borate (2-APB) on morphine analgesia and tolerance in rats. Forty-eight Wistar Albino male rats were included in the study and the rats were randomly divided into drug and control (saline) groups. To induce morphine tolerance, the rats were injected with 10 mg/kg morphine intraperitoneally for 7 days. After thermal analgesia tests, dorsal root ganglion (DRG) and cortex tissues were isolated. Proapoptotic mediators caspase-3 and 9, total oxidant status (TOS) and total antioxidant status (TAS) and ER stress proteins GRP78/BiP, ATF-6, p-IRE1 and pERK levels were measured by biochemical analysis of tissue homogenates. The findings showed that there was a significant decrease in morphine tolerance in rats administered ACA and 2-APB (p<0.05). In addition, biochemical tests revealed a significant decrease in ER stress proteins, proapoptotic biomarkers and TOS levels and a significant increase in TAS levels in DRG, thalamus and sensory cortex tissues (p<0.05). In conclusion, inhibition of TRPM2 cation channel by ACA and 2-APB reduces morphine tolerance by preventing ER stress and apoptosis. It may be possible to increase the analgesic potential of morphine by combined application with ACA and 2-APB in the clinic, but further experimental and molecular studies are needed.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138168"},"PeriodicalIF":2.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between individual differences in gait motor imagery and visuo-spatial working memory after stroke 脑卒中后步态运动意象与视觉空间工作记忆个体差异的关系

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-17 DOI: 10.1016/j.neulet.2025.138167

Kohei Kotegawa , Naoki Kuroda , Junya Sakata , Ren Fujii , Wataru Teramoto

{"title":"Association between individual differences in gait motor imagery and visuo-spatial working memory after stroke","authors":"Kohei Kotegawa , Naoki Kuroda , Junya Sakata , Ren Fujii , Wataru Teramoto","doi":"10.1016/j.neulet.2025.138167","DOIUrl":"10.1016/j.neulet.2025.138167","url":null,"abstract":"<div><div>Motor imagery is a mental process in which an individual internally simulates movements without actual motor execution. Gait motor imagery is associated with visuospatial working memory (VSWM) among young adults. This study investigates how individual differences in gait motor imagery ability among stroke patients are related to VSWM. Gait motor imagery of 12 S patients with right hemisphere damage and 12 healthy older adults were evaluated and compared in this study. Gait motor imagery ability was evaluated by comparing actual and mental walking times while manipulating path width, whereas VSWM ability was evaluated using the Corsi Block-Tapping task. The results revealed that VSWM ability could predict the accuracy of gait motor imagery for both stroke patients and healthy controls; those with higher VSWM ability exhibited more overestimation of mental walking time over actual walking time. Additionally, based on the results of dividing stroke participants into two groups depending on whether they had right prefrontal cortex (PFC) damage, stroke patients with right PFC damage had decreased VSWM, and underestimated mental walking over actual walking for all path widths compared to those with non-right PFC damage. These results suggest that gait motor imagery accuracy is associated with individual differences in VSWM ability, particularly in patients affected by right PFC damage.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138167"},"PeriodicalIF":2.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0