Neuroscience Letters最新文献_第10页

Nicotinamide adenine dinucleotide alleviates neuroinflammation in rats with traumatic brain injury 烟酰胺腺嘌呤二核苷酸减轻创伤性脑损伤大鼠神经炎症。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-22 DOI: 10.1016/j.neulet.2025.138178

Huancheng Wu , Mengli Jin , Jiandong Hu , Fenge Li

{"title":"Nicotinamide adenine dinucleotide alleviates neuroinflammation in rats with traumatic brain injury","authors":"Huancheng Wu , Mengli Jin , Jiandong Hu , Fenge Li","doi":"10.1016/j.neulet.2025.138178","DOIUrl":"10.1016/j.neulet.2025.138178","url":null,"abstract":"<div><h3>Objective</h3><div>To characterize the pathology and pathophysiological processes within 6 h after Traumatic brain injury (TBI) in rats, elucidate the neuroprotective effects and the underlying mechanisms of Nicotinamide Adenine Dinucleotide (NAD) in the early stage of TBI to explore the feasibility and clinical benefits of applying NAD directly to the localized injury after TBI.</div></div><div><h3>Material and Methods</h3><div>54 male Sprague-Dawley (SD) rats aged 6–8 weeks were randomly assigned equally to three groups, sham-operated surgery (SO) with saline treatment (SO + Saline), TBI with saline treatment (TBI + Saline), and TBI with 10 μM NAD treatment (TBI + NAD). The whole brain tissues were collected at 1, 3, and 6 h following the procedure. Levels of biomarkers for TBI including S100β, TNF-α, occludin, PPARβ/δ were measured.</div></div><div><h3>Results</h3><div>Significant neuroinflammation was observed in the rat brains after TBI, which peaked at 3 h following injury. Significant changes in S100β, TNF-α, PPARβ/δ, and occluding were also observed. Treatment with NAD significantly alleviated neuroinflammation at 1 h following TBI.</div></div><div><h3>Conclusions</h3><div>TBI caused severe neuroinflammation in rat brains, which peaked at 3 h following injury. Treatment with NAD alleviated neuroinflammation in TBI rats.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"852 ","pages":"Article 138178"},"PeriodicalIF":2.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the antidepressant effects of amantadine combined with amitriptyline in a chronic unpredictable mild stress model in mice 研究金刚烷胺联合阿米替林在小鼠慢性不可预测轻度应激模型中的抗抑郁作用

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138169

Vijay Kumar, Gaurav Doshi

{"title":"Investigating the antidepressant effects of amantadine combined with amitriptyline in a chronic unpredictable mild stress model in mice","authors":"Vijay Kumar, Gaurav Doshi","doi":"10.1016/j.neulet.2025.138169","DOIUrl":"10.1016/j.neulet.2025.138169","url":null,"abstract":"<div><div>Amantadine, an N-Methyl-D-Aspartate (NMDA) receptor antagonist traditionally used as an antiviral drug, was investigated for its potential antidepressant effect in combination with amitriptyline, a well-established tricyclic antidepressant, in a Chronic Unpredictable Mild Stress (CUMS) animal model of depression. This study aimed to evaluate the efficacy of this drug combination in alleviating depression-like symptoms. Behavioral assessments were conducted using the Forced Swim Test, Tail Suspension Test, Actophotometer test, and Sucrose Preference Test to measure depressive effect. Further biochemical analyses revealed a marked reduction in Brain-derived neurotrophic factor (BDNF) and norepinephrine (NE) levels in the CUMS group compared with the control group. BDNF is crucial for neuroplasticity, synaptic regulation, and neuronal survival and its reduced level is linked to the development of depression. Similarly, NE, a key neurotransmitter involved in mood regulation, is often decreased in depressive states. Conversely, the CUMS group exhibited a significant increase in Tumour necrosis factor-alpha (TNF-α) levels, indicating enhanced inflammatory response, which is also associated with depression. Treatment with the combination of amantadine and amitriptyline resulted in a significant antidepressant-like effect, as demonstrated by improved behavioral parameters and normalization of this biomarker the increase in BDNF and NE levels along with a reduction in TNF-α.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138169"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using MRI to Determine Drug Concentration in Convection-Enhanced Delivery: A Proof-of-Concept study 使用MRI来确定对流增强输送中的药物浓度：一项概念验证研究。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138170

Xiaojie Ding , Ming Qi , Yuan Zhou , Ying Qi , Di Chen , Xinyu Yang , Chunxia Ji , Yu Yao

{"title":"Using MRI to Determine Drug Concentration in Convection-Enhanced Delivery: A Proof-of-Concept study","authors":"Xiaojie Ding , Ming Qi , Yuan Zhou , Ying Qi , Di Chen , Xinyu Yang , Chunxia Ji , Yu Yao","doi":"10.1016/j.neulet.2025.138170","DOIUrl":"10.1016/j.neulet.2025.138170","url":null,"abstract":"<div><div>Convection-enhanced delivery (CED) bypasses the blood–brain barrier and avoids systemic exposure to the drug. However, systemic pharmacokinetic characteristics of a drug cannot be applied when delivered via CED. This study aims to provide a first proof-of-concept framework for noninvasively evaluating pharmacokinetics in CED. We investigated local concentration and the distribution of a gadolinium-based contrast agent in rat brains using magnetic resonance imaging (MRI). Standards of gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) were scanned on a 7.0 T MRI system in rat brain tissue suspension. <em>T</em><sub>1</sub> values were mapped and <em>T</em><sub>1</sub> relaxivity of Gd-DTPA was calculated. Subsequently, evaluation in live animals was performed by infusing Gd-DTPA into the rat striatum followed by scans for <em>T</em><sub>1</sub> mapping. The quantitative relationship between imaging data and Gd-DTPA concentration deduced from standard scans was used to determine the voxel-level concentration of Gd-DTPA in rat brains. Concentration maps were constructed from voxel-level concentration data. The Gd-DTPA concentration in tissue suspension and 1/ <em>T</em><sub>1</sub> showed a linear relationship with <em>R</em><sup>2</sup> of 0.9919 (p < 0.0001). The <em>T</em><sub>1</sub> relaxivity of Gd-DTPA at the experimental condition was 4.199 mM<sup>−1</sup> s<sup>−1</sup>. Within the rat brain parenchyma, the mean volume of distribution to initial volume ratio (Vd/Vi) of Gd-DTPA was calculated to be 6.02. Notably, the infusion center’s concentration exhibited a decreasing pattern, while the peripheral region’s concentration remained relatively stable over the observed duration. This study showed the spatial distribution of Gd-DTPA concentration and its temporal change, suggesting that using MRI to determine the Gd-DTPA concentration is feasible with good accuracy and data quality.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138170"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased anxiety-like behavior and trigeminal ganglion BDNF levels persist in rats with temporomandibular joint arthritis even after resolution of the nociceptive process 颞下颌关节关节炎大鼠的焦虑样行为和三叉神经节BDNF水平持续下降，即使在伤害过程解决后。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-19 DOI: 10.1016/j.neulet.2025.138166

Cristina Balensiefer Vicenzi , Dirson João Stein , Josimar Macedo de Castro , Beatriz Lima Silveira , Alanis da Silva Melo , Etiane Micheli Meyer Callai , Fernanda Visioli , Wolnei Caumo , Alexandre Silva de Quevedo , John K. Neubert , Iraci L.S. Torres

{"title":"Decreased anxiety-like behavior and trigeminal ganglion BDNF levels persist in rats with temporomandibular joint arthritis even after resolution of the nociceptive process","authors":"Cristina Balensiefer Vicenzi , Dirson João Stein , Josimar Macedo de Castro , Beatriz Lima Silveira , Alanis da Silva Melo , Etiane Micheli Meyer Callai , Fernanda Visioli , Wolnei Caumo , Alexandre Silva de Quevedo , John K. Neubert , Iraci L.S. Torres","doi":"10.1016/j.neulet.2025.138166","DOIUrl":"10.1016/j.neulet.2025.138166","url":null,"abstract":"<div><div>In this study, pain- and anxiety-like behaviors, locomotor and exploratory activity, histological and biomarker parameters were evaluated following induction of a temporomandibular joint (TMJ) arthritis model in rats. Twenty-two adult male Wistar rats were assigned to receive either saline (sham group) or Complete Freund’s Adjuvant (CFA − pain group) into the right TMJ. Mechanical allodynia was assessed using the facial electronic von Frey (VF) test, while thermal hyperalgesia was assessed using the Orofacial Pain Assessment Device (OPAD) assay. Open-field and plus-maze tests were used to assess locomotor and exploratory activity and anxiety-like behaviors, respectively. BDNF, IL-1β, and IL-10 levels were analyzed by ELISA in both the ipsilateral and contralateral trigeminal ganglion (TG). The tissues adjacent to the TMJ were histologically evaluated for the inflammatory process. According to distribution, data were analyzed by GEE, independent <em>t</em>-test, or Mann-Whitney test. Significance was set at P < 0.05. On the ninth day following CFA injection, pain-rats presented mechanical allodynia, which persisted until the twenty-first day, a decrease in the number of OPAD licks, decreased BDNF levels in the contralateral TG, and an increase in the ipsilateral TG BDNF and IL-1β levels, with inflammatory infiltrates in the tissues adjacent to the TMJ (27 days). TMJ arthritis also resulted in a reduction of the anxiety index (AI) after 26 days. This study reveals that this model is effective for examining chronic alterations related to TMJ arthritis and for identifying new anti-inflammatory drugs for pain management.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138166"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of the TRPM2 cation channel attenuates morphine tolerance by modulating endoplasmic reticulum stress and apoptosis in rats 抑制TRPM2阳离子通道通过调节内质网应激和细胞凋亡减弱吗啡耐受性。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-18 DOI: 10.1016/j.neulet.2025.138168

Arzuhan Cetindag Ciltas , Ercan Ozdemir , Handan Gunes , Aysegul Ozturk

{"title":"Inhibition of the TRPM2 cation channel attenuates morphine tolerance by modulating endoplasmic reticulum stress and apoptosis in rats","authors":"Arzuhan Cetindag Ciltas , Ercan Ozdemir , Handan Gunes , Aysegul Ozturk","doi":"10.1016/j.neulet.2025.138168","DOIUrl":"10.1016/j.neulet.2025.138168","url":null,"abstract":"<div><div>Opioid drugs such as morphine are frequently preferred drugs for severe pain in cancer and chronic diseases, but long-term use causes opioid tolerance. The mechanism of tolerance to opioids is quite complex and not fully understood. Our aim in this study was to investigate the effects of TRPM2 cation channel antagonists N-(p-amylcinnamoyl) anthranilic acid (ACA) and 2-aminoethoxydiphenyl borate (2-APB) on morphine analgesia and tolerance in rats. Forty-eight Wistar Albino male rats were included in the study and the rats were randomly divided into drug and control (saline) groups. To induce morphine tolerance, the rats were injected with 10 mg/kg morphine intraperitoneally for 7 days. After thermal analgesia tests, dorsal root ganglion (DRG) and cortex tissues were isolated. Proapoptotic mediators caspase-3 and 9, total oxidant status (TOS) and total antioxidant status (TAS) and ER stress proteins GRP78/BiP, ATF-6, p-IRE1 and pERK levels were measured by biochemical analysis of tissue homogenates. The findings showed that there was a significant decrease in morphine tolerance in rats administered ACA and 2-APB (p<0.05). In addition, biochemical tests revealed a significant decrease in ER stress proteins, proapoptotic biomarkers and TOS levels and a significant increase in TAS levels in DRG, thalamus and sensory cortex tissues (p<0.05). In conclusion, inhibition of TRPM2 cation channel by ACA and 2-APB reduces morphine tolerance by preventing ER stress and apoptosis. It may be possible to increase the analgesic potential of morphine by combined application with ACA and 2-APB in the clinic, but further experimental and molecular studies are needed.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138168"},"PeriodicalIF":2.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between individual differences in gait motor imagery and visuo-spatial working memory after stroke 脑卒中后步态运动意象与视觉空间工作记忆个体差异的关系

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-17 DOI: 10.1016/j.neulet.2025.138167

Kohei Kotegawa , Naoki Kuroda , Junya Sakata , Ren Fujii , Wataru Teramoto

{"title":"Association between individual differences in gait motor imagery and visuo-spatial working memory after stroke","authors":"Kohei Kotegawa , Naoki Kuroda , Junya Sakata , Ren Fujii , Wataru Teramoto","doi":"10.1016/j.neulet.2025.138167","DOIUrl":"10.1016/j.neulet.2025.138167","url":null,"abstract":"<div><div>Motor imagery is a mental process in which an individual internally simulates movements without actual motor execution. Gait motor imagery is associated with visuospatial working memory (VSWM) among young adults. This study investigates how individual differences in gait motor imagery ability among stroke patients are related to VSWM. Gait motor imagery of 12 S patients with right hemisphere damage and 12 healthy older adults were evaluated and compared in this study. Gait motor imagery ability was evaluated by comparing actual and mental walking times while manipulating path width, whereas VSWM ability was evaluated using the Corsi Block-Tapping task. The results revealed that VSWM ability could predict the accuracy of gait motor imagery for both stroke patients and healthy controls; those with higher VSWM ability exhibited more overestimation of mental walking time over actual walking time. Additionally, based on the results of dividing stroke participants into two groups depending on whether they had right prefrontal cortex (PFC) damage, stroke patients with right PFC damage had decreased VSWM, and underestimated mental walking over actual walking for all path widths compared to those with non-right PFC damage. These results suggest that gait motor imagery accuracy is associated with individual differences in VSWM ability, particularly in patients affected by right PFC damage.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138167"},"PeriodicalIF":2.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of filamin A-interacting protein (FILIP) results in a weak grip strength and abnormal responses to nociceptive stimulation 丝素a相互作用蛋白（FILIP）的缺失导致握力弱和对伤害性刺激的异常反应。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-15 DOI: 10.1016/j.neulet.2025.138158

Hideshi Yagi , Keizo Takao , Satoko Hattori , Yusuke Minato , Sachi Kuwahara-Otani , Seishi Maeda , Koichi Noguchi , Tsuyoshi Miyakawa , Makoto Sato

{"title":"Deletion of filamin A-interacting protein (FILIP) results in a weak grip strength and abnormal responses to nociceptive stimulation","authors":"Hideshi Yagi , Keizo Takao , Satoko Hattori , Yusuke Minato , Sachi Kuwahara-Otani , Seishi Maeda , Koichi Noguchi , Tsuyoshi Miyakawa , Makoto Sato","doi":"10.1016/j.neulet.2025.138158","DOIUrl":"10.1016/j.neulet.2025.138158","url":null,"abstract":"<div><div>Filamin A-interacting protein (FILIP in mice, FILIP1 in humans) was first identified as a protein that negatively controls neuronal migration in rodents, and was subsequently demonstrated to be pivotal for the development of the neocortex. In the previous study, we generated FILIP knockout mice to investigate the in vivo functions of FILIP in cortical development. Since FILIP mRNA is widely expressed in the body, we systematically examined FILIP-knockout mice to determine the functions of FILIP throughout the body. Our results showed that FILIP-knockout mice exhibited weak grip strength and sensory abnormalities. Interestingly, we also found that FILIP was expressed in a subset of neurons in the dorsal root ganglion (DRG). Recent research has reported that <em>FILIP1</em> mutations lead to severe neurological and musculoskeletal abnormalities, resulting in the proposal of a new disease entity, termed FILIP1opathy. It is expected that our FILIP-knockout mice could be used as a model for the pathological investigation of FILIP1opathy.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138158"},"PeriodicalIF":2.5,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of opioid receptors in the anti-allodynic effect of local montelukast in a rat chronic constriction injury of sciatic nerve model 阿片受体在孟鲁司特局部抗坐骨神经慢性收缩损伤模型中的作用。

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-15 DOI: 10.1016/j.neulet.2025.138165

Behnam Ghorbanzadeh , Behnam Azizolahi , Mohammad Amin Behmanesh , Parsa Forouhar , Ali Foroughinia , Mohadeseh Nabizadeh

{"title":"The role of opioid receptors in the anti-allodynic effect of local montelukast in a rat chronic constriction injury of sciatic nerve model","authors":"Behnam Ghorbanzadeh , Behnam Azizolahi , Mohammad Amin Behmanesh , Parsa Forouhar , Ali Foroughinia , Mohadeseh Nabizadeh","doi":"10.1016/j.neulet.2025.138165","DOIUrl":"10.1016/j.neulet.2025.138165","url":null,"abstract":"<div><div>Neuropathic pain is a debilitating and chronic condition that results from damage to the peripheral and central nervous system. The inflammatory mediators such as leukotrienes, and opioidergic pathways are involved in the neuropathic pain generation. The present study aimed to determine the effect of local montelukast and the role of opioid receptors using chronic constriction injury (CCI) of the sciatic nerve in rats. Our results showed that montelukast (1–10 mcg/paw) or morphine (1 and 10 mcg/paw) attenuated the mechanical and cold allodynia at day 7 and 14 post-CCI. The effect of montelukast was attenuated by local pre-treatment with naloxone (20 mcg/paw), and was augmented by an ineffective dose of morphine. Also, the histopathological investigation showed the peripheral anti-inflammatory effect of montelukast in the sciatic-injured paw. Moreover, spinal cord mu-opioid receptor mRNA decreased, and kappa-opioid receptor mRNA increased in rats 14 days after CCI by RT-PCR analyses. However, the administration of montelukast on days 7 and 14 after CCI reversed the observed changes in opioid receptors. Our findings suggested that local montelukast can attenuate neuropathic pain, at least in part, through the peripheral opioid receptors, peripheral anti-inflammatory, and also spinal mu- and kappa-opioid receptors. So, local montelukast could be a novel therapeutic strategy for alleviating neuropathic pain.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"851 ","pages":"Article 138165"},"PeriodicalIF":2.5,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic anxiolytic-like effect of CPPG and harmaline in non-stressed and acute restraint stress (ARS) mice CPPG和丙烯碱对非应激和急性约束应激小鼠的协同抗焦虑样作用

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-10 DOI: 10.1016/j.neulet.2025.138157

Nazahnin Hasan-Kareem , Sakineh Alijanpour , Mohammad-Reza Zarrindast , Fatemeh Khakpai

{"title":"Synergistic anxiolytic-like effect of CPPG and harmaline in non-stressed and acute restraint stress (ARS) mice","authors":"Nazahnin Hasan-Kareem , Sakineh Alijanpour , Mohammad-Reza Zarrindast , Fatemeh Khakpai","doi":"10.1016/j.neulet.2025.138157","DOIUrl":"10.1016/j.neulet.2025.138157","url":null,"abstract":"<div><div>Many studies revealed the role of metabotropic glutamate receptors (mGluRs) and harmaline in the modulation of anxiety-related behaviors. This study aimed to determine a possible interaction between harmaline and group III mGluR on the modulation of anxiety-correlated behaviors. The left lateral ventricle of male mice was unilaterally cannulated. Acute restraint stress (ARS) was induced by movement restraint for 4 h. Anxiety-like behaviors were measured using an elevated plus maze. The results showed that induction of ARS during 4 h reduced the percentage of time spent in open arms (%OAT) and percentage of entries to open arms (%OAE) without changing locomotor activity, indicating anxiogenic-like responses. Intraperitoneal (i.p.) administration of harmaline (2 mg/kg) increased %OAT in non-stressed and ARS mice, presenting anxiolytic-like responses. Intracerebroventricular (i.c.v.) infusion of CPPG (potent group III mGlu antagonist, 70 µg/mouse) induced anxiolytic-like behavior due to the augmentation of %OAT in non-stressed and ARS mice. Co-treatment of CPPG (70 µg/mouse, i.c.v.) along with harmaline (1 mg/kg, i.p) induced an anxiolytic-like effect. I.c.v. infusion of L-AP4 (selective group III mGlu agonist) or co-administration of it along harmaline had no significant effect on anxiety-like behaviors both in non-stressed and ARS mice. When harmaline and CPPG were co-administrated, CPPG potentiated the anxiolytic-like behavior induced by harmaline in non-stressed and ARS mice. The results revealed a synergistic effect between CPPG and harmaline on the induction of anxiolytic-like effect in non-stressed and ARS mice. Our results indicated an interaction between harmaline and group III mGluR on the modulation of anxiety-like responses in non-stressed and ARS mice.</div></div>","PeriodicalId":19290,"journal":{"name":"Neuroscience Letters","volume":"850 ","pages":"Article 138157"},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of STAT1 improved learning and memory impairments in aging mice STAT1的下调改善了衰老小鼠的学习和记忆障碍

IF 2.5 4区医学

Neuroscience Letters Pub Date : 2025-02-09 DOI: 10.1016/j.neulet.2025.138155

Xiao Li , Yao Xu , Ting Li , Bocheng Xiong , Xifei Yang , Yan Feng

引用次数: 0