烟酰胺腺嘌呤二核苷酸减轻创伤性脑损伤大鼠神经炎症。

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-02-22 DOI:10.1016/j.neulet.2025.138178

Huancheng Wu , Mengli Jin , Jiandong Hu , Fenge Li

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引用次数: 0

摘要

目的：研究大鼠创伤性脑损伤（TBI）后6 h内的病理生理过程，阐明烟酰胺腺嘌呤二核苷酸（Nicotinamide Adenine Dinucleotide， NAD）在TBI早期的神经保护作用及其机制，探讨NAD直接应用于TBI后局部损伤的可行性及临床益处。材料与方法：将54只6-8 周龄雄性SD大鼠随机分为假手术（SO）加生理盐水治疗组（SO + saline）、脑外伤加生理盐水治疗组（TBI + saline）和脑外伤加10 μM NAD治疗组（TBI + NAD）。在手术后1、3和6 h收集全脑组织。测量TBI生物标志物水平，包括S100β、TNF-α、occludin、PPARβ/δ。结果：大鼠脑外伤后出现明显的神经炎症反应，炎症反应在损伤后3 h达到高峰。S100β、TNF-α、PPARβ/δ和闭塞性也有显著变化。NAD治疗可显著缓解TBI后1 h的神经炎症。结论：TBI可引起大鼠脑内严重的神经炎症，损伤后炎症反应在3 h时达到高峰。NAD治疗可减轻TBI大鼠的神经炎症。

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Nicotinamide adenine dinucleotide alleviates neuroinflammation in rats with traumatic brain injury

Objective

To characterize the pathology and pathophysiological processes within 6 h after Traumatic brain injury (TBI) in rats, elucidate the neuroprotective effects and the underlying mechanisms of Nicotinamide Adenine Dinucleotide (NAD) in the early stage of TBI to explore the feasibility and clinical benefits of applying NAD directly to the localized injury after TBI.

Material and Methods

54 male Sprague-Dawley (SD) rats aged 6–8 weeks were randomly assigned equally to three groups, sham-operated surgery (SO) with saline treatment (SO + Saline), TBI with saline treatment (TBI + Saline), and TBI with 10 μM NAD treatment (TBI + NAD). The whole brain tissues were collected at 1, 3, and 6 h following the procedure. Levels of biomarkers for TBI including S100β, TNF-α, occludin, PPARβ/δ were measured.

Results

Significant neuroinflammation was observed in the rat brains after TBI, which peaked at 3 h following injury. Significant changes in S100β, TNF-α, PPARβ/δ, and occluding were also observed. Treatment with NAD significantly alleviated neuroinflammation at 1 h following TBI.

Conclusions

TBI caused severe neuroinflammation in rat brains, which peaked at 3 h following injury. Treatment with NAD alleviated neuroinflammation in TBI rats.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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