Non-Coding RNA最新文献_第7页

Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages. 单核细胞/巨噬细胞炎症小体激活中长非编码RNA的系统分析。

IF 4.3

Non-Coding RNA Pub Date : 2023-08-25 DOI: 10.3390/ncrna9050050

Na Qian, Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Sarah Rennie, Shizuka Uchida

{"title":"Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages.","authors":"Na Qian, Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Sarah Rennie, Shizuka Uchida","doi":"10.3390/ncrna9050050","DOIUrl":"https://doi.org/10.3390/ncrna9050050","url":null,"abstract":"The NLRP3 inflammasome plays a pivotal role in regulating inflammation and immune responses. Its activation can lead to an inflammatory response and pyroptotic cell death. This is beneficial in the case of infections, but excessive activation can lead to chronic inflammation and tissue damage. Moreover, while most of the mammalian genome is transcribed as RNAs, only a small fraction codes for proteins. Among non-protein-coding RNAs, long non-coding RNAs (lncRNAs) have been shown to play key roles in regulating gene expression and cellular processes. They interact with DNA, RNAs, and proteins, and their dysregulation can provide insights into disease mechanisms, including NLRP3 inflammasome activation. Here, we systematically analyzed previously published RNA sequencing (RNA-seq) data of NLRP3 inflammasome activation in monocytes/macrophages to uncover inflammasome-regulated lncRNA genes. To uncover the functional importance of inflammasome-regulated lncRNA genes, one inflammasome-regulated lncRNA, ENSG00000273124, was knocked down in an in vitro model of macrophage polarization. The results indicate that silencing of ENSG00000273124 resulted in the up-regulation tumor necrosis factor (TNF), suggesting that this lncRNA might be involved in pro-inflammatory response in macrophages. To make our analyzed data more accessible, we developed the web database InflammasomeDB.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and Obstacles in Using CRISPR/Cas9 Technology for Non-Coding RNA Gene Knockout in Human Mesenchymal Stromal Cells. CRISPR/Cas9技术用于人间充质干细胞非编码RNA基因敲除的进展和障碍。

IF 4.3

Non-Coding RNA Pub Date : 2023-08-24 DOI: 10.3390/ncrna9050049

Nataliya Basalova, Maria Illarionova, Mariya Skryabina, Maksim Vigovskiy, Anastasia Tolstoluzhinskaya, Alexandra Primak, Elizaveta Chechekhina, Vadim Chechekhin, Maxim Karagyaur, Anastasia Efimenko

{"title":"Advances and Obstacles in Using CRISPR/Cas9 Technology for Non-Coding RNA Gene Knockout in Human Mesenchymal Stromal Cells.","authors":"Nataliya Basalova, Maria Illarionova, Mariya Skryabina, Maksim Vigovskiy, Anastasia Tolstoluzhinskaya, Alexandra Primak, Elizaveta Chechekhina, Vadim Chechekhin, Maxim Karagyaur, Anastasia Efimenko","doi":"10.3390/ncrna9050049","DOIUrl":"https://doi.org/10.3390/ncrna9050049","url":null,"abstract":"Non-coding RNA (ncRNAs) genes have attracted increasing attention in recent years due to their widespread involvement in physiological and pathological processes and regulatory networks. The study of the function and molecular partners of ncRNAs opens up opportunities for the early diagnosis and treatment of previously incurable diseases. However, the classical \"loss-of-function\" approach in ncRNA function analysis is challenged due to some specific issues. Here, we have studied the potency of two CRISPR/Cas9 variants, wild-type (SpCas9wt) and nickase (SpCas9D10A) programmable nucleases, for the editing of extended DNA sequences in human mesenchymal stromal cells (MSCs). Editing the genes of fibrosis-related hsa-miR-21-5p and hsa-miR-29c-3p, we have shown that a pair of SpCas9D10A molecules can effectively disrupt miRNA genes within the genomes of MSCs. This leads not only to a decrease in the level of knockout miRNA in MSCs and MSC-produced extracellular vesicles, but also to a change in cell physiology and the antifibrotic properties of the cell secretome. These changes correlate well with previously published data for the knockdown of certain miRNAs. The proposed approach can be used to knock out ncRNA genes within the genomes of MSCs or similar cell types in order to study their function in biological processes.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41143000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of lncRNAs in Lupinus mutabilis (Tarwi) and Their Potential Role in Drought Response. 突变狼疮lncRNA的分析及其在干旱反应中的潜在作用。

IF 4.3

Non-Coding RNA Pub Date : 2023-08-23 DOI: 10.3390/ncrna9050048

Manuel Hidalgo, Cynthia Ramos, Gaston Zolla

{"title":"Analysis of lncRNAs in Lupinus mutabilis (Tarwi) and Their Potential Role in Drought Response.","authors":"Manuel Hidalgo, Cynthia Ramos, Gaston Zolla","doi":"10.3390/ncrna9050048","DOIUrl":"https://doi.org/10.3390/ncrna9050048","url":null,"abstract":"Lupinus mutabilis is a legume with high agronomic potential and available transcriptomic data for which lncRNAs have not been studied. Therefore, our objective was to identify, characterize, and validate the drought-responsive lncRNAs in L. mutabilis. To achieve this, we used a multilevel approach based on lncRNA prediction, annotation, subcellular location, thermodynamic characterization, structural conservation, and validation. Thus, 590 lncRNAs were identified by at least two algorithms of lncRNA identification. Annotation with the PLncDB database showed 571 lncRNAs unique to tarwi and 19 lncRNAs with homology in 28 botanical families including Solanaceae (19), Fabaceae (17), Brassicaceae (17), Rutaceae (17), Rosaceae (16), and Malvaceae (16), among others. In total, 12 lncRNAs had homology in more than 40 species. A total of 67% of lncRNAs were located in the cytoplasm and 33% in exosomes. Thermodynamic characterization of S03 showed a stable secondary structure with -105.67 kcal/mol. This structure included three regions, with a multibranch loop containing a hairpin with a SECIS-like element. Evaluation of the structural conservation by CROSSalign revealed partial similarities between L. mutabilis (S03) and S. lycopersicum (Solyc04r022210.1). RT-PCR validation demonstrated that S03 was upregulated in a drought-tolerant accession of L. mutabilis. Finally, these results highlighted the importance of lncRNAs in tarwi improvement under drought conditions.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation into the Role of Long-Non-Coding RNA MIAT in Leukemia. 长链非编码RNA MIAT在白血病中的作用研究。

IF 4.3

Non-Coding RNA Pub Date : 2023-08-11 DOI: 10.3390/ncrna9040047

Alessia Ostini, Mirna Mourtada-Maarabouni

{"title":"Investigation into the Role of Long-Non-Coding RNA MIAT in Leukemia.","authors":"Alessia Ostini, Mirna Mourtada-Maarabouni","doi":"10.3390/ncrna9040047","DOIUrl":"https://doi.org/10.3390/ncrna9040047","url":null,"abstract":"Myocardial Infarction Associated Transcript (MIAT) is a nuclear long non-coding RNA (LncRNA) with four different splicing variants. MIAT dysregulation is associated with carcinogenesis, mainly acting as an oncogene regulating cellular growth, invasion, and metastasis. The aim of the current study is to investigate the role of MIAT in the regulation of T and chronic myeloid leukemic cell survival. To this end, MIAT was silenced using MIAT-specific siRNAs in leukemic cell lines, and functional assays were performed thereafter. This investigation also aims to investigate the effects of MIAT silencing on the expression of core genes involved in cancer. Functional studies and gene expression determination confirm that MIAT knockdown not only affects short- and long-term survival and the apoptosis of leukemic cells but also plays a pivotal role in the alteration of key genes involved in cancer, including c-MYC and HIF-1A. Our observations suggest that MIAT could act as an oncogene and it has the potential to be used not only as a reliable biomarker for leukemia, but also be employed for prognostic and therapeutic purposes.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10158892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Modifications and Novel Protein-Binding Sites in Pre-miR-675-Explaining Its Regulatory Mechanism in Carcinogenesis. Pre-miR-675的结构修饰和新型蛋白结合位点--解释其在致癌过程中的调控机制

IF 3.6

Non-Coding RNA Pub Date : 2023-08-10 DOI: 10.3390/ncrna9040045

Abhishek Dey

{"title":"Structural Modifications and Novel Protein-Binding Sites in Pre-miR-675-Explaining Its Regulatory Mechanism in Carcinogenesis.","authors":"Abhishek Dey","doi":"10.3390/ncrna9040045","DOIUrl":"10.3390/ncrna9040045","url":null,"abstract":"Pre-miR-675 is a microRNA expressed from the exon 1 of H19 long noncoding RNA, and the atypical expression of pre-miR-675 has been linked with several diseases and disorders including cancer. To execute its function inside the cell, pre-miR-675 is folded into a particular conformation, which aids in its interaction with several other biological molecules. However, the exact folding dynamics of pre-miR-675 and its protein-binding motifs are currently unknown. Moreover, how H19 lncRNA and pre-miR-675 crosstalk and modulate each other's activities is also unclear. The detailed structural analysis of pre-miR-675 in this study determines its earlier unknown conformation and identifies novel protein-binding sites on pre-miR-675, thus making it an excellent therapeutic target against cancer. Co-folding analysis between H19 lncRNA and pre-miR-675 determine structural transformations in pre-miR-675, thus describing the earlier unknown mechanism of interaction between these two molecules. Comprehensively, this study details the conformation of pre-miR-675 and its protein-binding sites and explains its relationship with H19 lncRNA, which can be interpreted to understand the role of pre-miR-675 in the development and progression of tumorigenesis and designing new therapeutics against cancers.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods and Tools in RNA Biology. RNA生物学的方法和工具。

IF 4.3

Non-Coding RNA Pub Date : 2023-08-10 DOI: 10.3390/ncrna9040046

Mirolyuba Ilieva, Shizuka Uchida

引用次数: 0

Effects of Controlled Ozone Exposure on Circulating microRNAs and Vascular and Coagulation Biomarkers: A Mediation Analysis. 受控臭氧暴露对循环 microRNAs 以及血管和凝血生物标志物的影响：中介分析

IF 4.3

Non-Coding RNA Pub Date : 2023-08-01 DOI: 10.3390/ncrna9040043

Hao Chen, Syed Masood, Ana G Rappold, David Diaz-Sanchez, James M Samet, Haiyan Tong

{"title":"Effects of Controlled Ozone Exposure on Circulating microRNAs and Vascular and Coagulation Biomarkers: A Mediation Analysis.","authors":"Hao Chen, Syed Masood, Ana G Rappold, David Diaz-Sanchez, James M Samet, Haiyan Tong","doi":"10.3390/ncrna9040043","DOIUrl":"10.3390/ncrna9040043","url":null,"abstract":"Exposure to ozone (O3) is associated with adverse respiratory and cardiovascular outcomes. Alterations in circulating microRNAs (miRNAs) may contribute to the adverse vascular effects of O3 exposure through inter-cellular communication resulting in post-transcriptional regulation of messenger RNAs by miRNAs. In this study, we investigated whether O3 exposure induces alterations in circulating miRNAs that can mediate effects on downstream vascular and coagulation biomarkers. Twenty-three healthy male adults were exposed on successive days to filtered air and 300 ppb O3 for 2 h. Circulating miRNA and protein biomarkers were quantified after each exposure session. The data were subjected to mixed-effects model and mediation analyses for the statistical analyses. The results showed that the expression level of multiple circulating miRNAs (e.g., miR-19a-3p, miR-34a-5p) was significantly associated with O3 exposure. Pathway analysis showed that these miRNAs were predictive of changing levels of downstream biomarkers [e.g., D-dimer, C-reactive protein, tumor necrosis factor α (TNFα)]. Mediation analysis showed that miR-19a-3p may be a significant mediator of O3-exposure-induced changes in blood TNFα levels [0.08 (0.01, 0.15), p = 0.02]. In conclusion, this preliminary study showed that O3 exposure of healthy male adults resulted in changes in circulating miRNAs, some of which may mediate vascular effects of O3 exposure.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long Non-Coding RNA Signatures in Lymphopoiesis and Lymphoid Malignancies. 淋巴细胞生成和淋巴恶性肿瘤中的长非编码 RNA 特征

IF 3.6

Non-Coding RNA Pub Date : 2023-08-01 DOI: 10.3390/ncrna9040044

Hamed Baghdadi, Reza Heidari, Mahdi Zavvar, Nazanin Ahmadi, Mehdi Shakouri Khomartash, Mahmoud Vahidi, Mojgan Mohammadimehr, Davood Bashash, Mahdi Ghorbani

{"title":"Long Non-Coding RNA Signatures in Lymphopoiesis and Lymphoid Malignancies.","authors":"Hamed Baghdadi, Reza Heidari, Mahdi Zavvar, Nazanin Ahmadi, Mehdi Shakouri Khomartash, Mahmoud Vahidi, Mojgan Mohammadimehr, Davood Bashash, Mahdi Ghorbani","doi":"10.3390/ncrna9040044","DOIUrl":"10.3390/ncrna9040044","url":null,"abstract":"Lymphoid cells play a critical role in the immune system, which includes three subgroups of T, B, and NK cells. Recognition of the complexity of the human genetics transcriptome in lymphopoiesis has revolutionized our understanding of the regulatory potential of RNA in normal lymphopoiesis and lymphoid malignancies. Long non-coding RNAs (lncRNAs) are a class of RNA molecules greater than 200 nucleotides in length. LncRNAs have recently attracted much attention due to their critical roles in various biological processes, including gene regulation, chromatin organization, and cell cycle control. LncRNAs can also be used for cell differentiation and cell fate, as their expression patterns are often specific to particular cell types or developmental stages. Additionally, lncRNAs have been implicated in lymphoid differentiation, such as regulating T-cell and B-cell development, and their expression has been linked to immune-associated diseases such as leukemia and lymphoma. In addition, lncRNAs have been investigated as potential biomarkers for diagnosis, prognosis, and therapeutic response to disease management. In this review, we provide an overview of the current knowledge about the regulatory role of lncRNAs in physiopathology processes during normal lymphopoiesis and lymphoid leukemia.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":3.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between Long Non-Coding RNA and Spliceosomal microRNA as a Novel Biomarker for Cancer. 长链非编码RNA与剪接小RNA之间的串扰作为一种新的癌症生物标志物。

IF 4.3

Non-Coding RNA Pub Date : 2023-07-31 DOI: 10.3390/ncrna9040042

Maram Arafat, Ruth Sperling

{"title":"Crosstalk between Long Non-Coding RNA and Spliceosomal microRNA as a Novel Biomarker for Cancer.","authors":"Maram Arafat, Ruth Sperling","doi":"10.3390/ncrna9040042","DOIUrl":"https://doi.org/10.3390/ncrna9040042","url":null,"abstract":"Non-coding RNAs (ncRNAs) play diverse roles in regulating cellular processes and have been implicated in pathological conditions, including cancer, where interactions between ncRNAs play a role. Relevant here are (i) microRNAs (miRNAs), mainly known as negative regulators of gene expression in the cytoplasm. However, identification of miRNAs in the nucleus suggested novel nuclear functions, and (ii) long non-coding RNA (lncRNA) regulates gene expression at multiple levels. The recent findings of miRNA in supraspliceosomes of human breast and cervical cancer cells revealed new candidates of lncRNA targets. Here, we highlight potential cases of crosstalk between lncRNA and supraspliceosomal miRNA expressed from the same genomic region, having complementary sequences. Through RNA:RNA base pairing, changes in the level of one partner (either miRNA or lncRNA), as occur in cancer, could affect the level of the other, which might be involved in breast and cervical cancer. An example is spliceosomal mir-7704 as a negative regulator of the oncogenic lncRNA HAGLR. Because the expression of spliceosomal miRNA is cell-type-specific, the list of cis-interacting lncRNA:spliceosomal miRNA presented here is likely just the tip of the iceberg, and such interactions are likely relevant to additional cancers. We thus highlight the potential of lncRNA:spliceosomal miRNA interactions as novel targets for cancer diagnosis and therapies.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Conservation and Targets of miR-71: A Systematic Review and Meta-Analysis. miR-71的保护和目标:一项系统综述和荟萃分析。

IF 4.3

Non-Coding RNA Pub Date : 2023-07-26 DOI: 10.3390/ncrna9040041

Devin Naidoo, Ryan Brennan, Alexandre de Lencastre

{"title":"Conservation and Targets of miR-71: A Systematic Review and Meta-Analysis.","authors":"Devin Naidoo, Ryan Brennan, Alexandre de Lencastre","doi":"10.3390/ncrna9040041","DOIUrl":"https://doi.org/10.3390/ncrna9040041","url":null,"abstract":"MicroRNAs (miRNAs) perform a pivotal role in the regulation of gene expression across the animal kingdom. As negative regulators of gene expression, miRNAs have been shown to function in the genetic pathways that control many biological processes and have been implicated in roles in human disease. First identified as an aging-associated gene in C. elegans, miR-71, a miRNA, has a demonstrated capability of regulating processes in numerous different invertebrates, including platyhelminths, mollusks, and insects. In these organisms, miR-71 has been shown to affect a diverse range of pathways, including aging, development, and immune response. However, the exact mechanisms by which miR-71 regulates these pathways are not completely understood. In this paper, we review the identified functions of miR-71 across multiple organisms, including identified gene targets, pathways, and the conditions which affect regulatory action. Additionally, the degree of conservation of miR-71 in the evaluated organisms and the conservation of their predicted binding sites in target 3' UTRs was measured. These studies may provide an insight on the patterns, interactions, and conditions in which miR-71 is able to exert genotypic and phenotypic influence.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0