Neuroscience最新文献_第5页

Short and long-term blockades of adenosine 2A, 5-HT2A, and 5-HT7 receptors induce apoptosis, reduce proliferation, and show differential effects on miR-27b-3p expression in neuroblastoma cell lines 短期和长期阻断腺苷 2A、5-HT2A 和 5-HT7 受体可诱导神经母细胞瘤细胞株凋亡、减少增殖，并对 miR-27b-3p 的表达产生不同影响。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-14 DOI: 10.1016/j.neuroscience.2024.11.032

Kemal Erdem Basaran , Seyda Korkmaz , Güzide Satır-Basaran , Hasan Salkın

{"title":"Short and long-term blockades of adenosine 2A, 5-HT2A, and 5-HT7 receptors induce apoptosis, reduce proliferation, and show differential effects on miR-27b-3p expression in neuroblastoma cell lines","authors":"Kemal Erdem Basaran , Seyda Korkmaz , Güzide Satır-Basaran , Hasan Salkın","doi":"10.1016/j.neuroscience.2024.11.032","DOIUrl":"10.1016/j.neuroscience.2024.11.032","url":null,"abstract":"<div><div>The first of our aims in this study was to investigate the effects of 5-HT2AR, 5-HT7R, and A2AR blockades on miR-27b-3p expression in the short and long-term in neuroblastoma cells. Our second aim was to reduce the expression of pERK and suppress proliferation by blocking the 5-HT2AR with ketanserin. Our third aim was to reduce the expression of pAKT and induce apoptosis by blocking the A2AR and 5-HT7R with MSX3 and SB269970. Thus, we aimed to investigate the therapeutic efficacy of ketanserin, MSX3 and SB269970, individually or in combination, on neuroblastoma cells.</div><div>We found that short and long-term blockades of A2A, 5-HT2A, and 5-HT7 receptors had different effects on miR-27b-3p expression. Blockade of A2AR and 5-HT7R with MSX3 and SB269970 decreased miR-27b-3p expression in the short term while increasing it in the long term. Ketanserin increased miR-27b-3p expression in both the short and long term. When 5-HT2AR was blocked with ketanserin, no significant difference was observed in pERK expression and proliferation in the short term. In contrast, a substantial decrease in pERK expression and proliferation was detected in the long term. Our findings show that the MSX3 + SB269970 dual combination and ketanserin + MSX3 + SB269970 triple combination are especially critical in suppressing pAKT expression in the long term. These findings showed that pAKT protein expression induced apoptosis due to decreased in neuroblastoma cells.</div><div>Our study provides the first evidence for the relationships between ketanserin/miR-27b-3p/pERK, MSX3/miR-27b-3p/pAKT, and SB269970/miR-27b-3p/pAKT in neuroblastoma cells. Ketanserin, MSX3, and SB269970 drug combinations may be promising therapeutic agents in neuroblastoma cells.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"563 ","pages":"Pages 212-221"},"PeriodicalIF":2.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eye movement disorders: A new approach to preliminary screening of Parkinson’s disease 眼球运动障碍：帕金森病初步筛查的新方法。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-14 DOI: 10.1016/j.neuroscience.2024.11.023

Han Li , Chengqian Li , Wenqi Ma , Kunpeng Qin , Zihan Wang , Binghui Hou , Anmu Xie

{"title":"Eye movement disorders: A new approach to preliminary screening of Parkinson’s disease","authors":"Han Li , Chengqian Li , Wenqi Ma , Kunpeng Qin , Zihan Wang , Binghui Hou , Anmu Xie","doi":"10.1016/j.neuroscience.2024.11.023","DOIUrl":"10.1016/j.neuroscience.2024.11.023","url":null,"abstract":"<div><div>To investigate the characteristics and diagnostic values of the eye movement disorders in patients with Parkinson’s disease (PD-EMDs), this cross-sectional study enrolled 127 Chinese patients with PD and 80 healthy controls, and divided them into training and validation sets based on enrollment time. Performance in the five oculomotor paradigms was assessed using an infrared pupil and a corneal reflection tracking device. The primary characteristics of PD-EMDs were elucidated as inaccurate fixation with high deviation (frequency and total quantity); inaccurate saccades with delayed reaction and low velocity; saccadic pursuit with high deviation, delayed reaction, and velocity; and decreased visual search ability. Subgroup comparison shows that PD-EMDs can be related with PD stages, motor subtypes, frozen gait, and drowsiness. Finally, we developed and externally validated a model for PD preliminary screening using multivariate stepwise logistic regression analysis, comprising four oculomotor parameters (fixation accuracy, pro-saccade velocity, anti-saccade accuracy, and visual search duration), cognition score and educational years. The model has good feasibility with satisfactory performance on the receiver operating characteristic, calibration, and decision curves, and broad clinical applicability with better discrimination for more advanced PD patients and non-tremor-dominant PD patients. A nomogram was created to make the model more user-friendly in the clinical setting. Overall, we have demonstrated the presence of PD-EMDs and their prospective value for PD preliminary screening.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"563 ","pages":"Pages 202-211"},"PeriodicalIF":2.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypocretin modulation of behavioral coping strategies for social stress 下视蛋白对社会压力行为应对策略的调节。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-14 DOI: 10.1016/j.neuroscience.2024.11.031

Pei X. Luo, Brian C. Trainor

{"title":"Hypocretin modulation of behavioral coping strategies for social stress","authors":"Pei X. Luo, Brian C. Trainor","doi":"10.1016/j.neuroscience.2024.11.031","DOIUrl":"10.1016/j.neuroscience.2024.11.031","url":null,"abstract":"<div><div>Best known for promoting wakefulness and arousal, the neuropeptide hypocretin (Hcrt) also plays an important role in mediating stress responses, including social stress. However, central and systemic manipulation of the Hcrt system has produced diverse behavioral outcomes in animal models. In this review, we first focus on studies where similar manipulations of the Hcrt system led to divergent coping behaviors. We hypothesize that Hcrt differentially facilitates active and passive coping behaviors in response to social stress by acting in different brain regions and on different cell types. We then focus on region and cell type-specific effects of Hcrt in the ventral pallidum, lateral habenula, ventral tegmental area, nucleus accumbens, amygdala, and bed nucleus of the stria terminalis. Overall, the evidence suggests that rather than enhancing or inhibiting behavioral responses to social stress, Hcrt may signal the heightened arousal associated with stressful contexts. The resulting behavioral effects depend on which circuits Hcrt release occurs in and which receptor types are activated. Further study is needed to determine how and why circuit specific activation of Hcrt neurons occurs.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 126-134"},"PeriodicalIF":2.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semaglutide promotes the transition of microglia from M1 to M2 type to reduce brain inflammation in APP/PS1/tau mice 塞马鲁肽能促进小胶质细胞从M1型向M2型转变，从而减轻APP/PS1/tau小鼠的脑部炎症。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-14 DOI: 10.1016/j.neuroscience.2024.11.022

Zhao-Jun Wang , Wei-Na Han , Shi-Fan Chai , Yan Li , Chao-Jing Fu , Chen-Fang Wang , Hong-Yan Cai , Xin-Yi Li , Xiao Wang , Christian Hölscher , Mei-Na Wu

{"title":"Semaglutide promotes the transition of microglia from M1 to M2 type to reduce brain inflammation in APP/PS1/tau mice","authors":"Zhao-Jun Wang , Wei-Na Han , Shi-Fan Chai , Yan Li , Chao-Jing Fu , Chen-Fang Wang , Hong-Yan Cai , Xin-Yi Li , Xiao Wang , Christian Hölscher , Mei-Na Wu","doi":"10.1016/j.neuroscience.2024.11.022","DOIUrl":"10.1016/j.neuroscience.2024.11.022","url":null,"abstract":"<div><div>A growing number of studies show that the diabetes drug Semaglutide is neuroprotective in Alzheimer’s disease (AD) animal models, but its mode of action is not fully understood. In order to explore the mechanism of Semaglutide, 7-month-old APP/PS1/tau transgenic (3xTg) mice and wild-type (WT) mice were randomly divided into four groups: control group (WT + PBS), AD model group (3xTg + PBS), Semaglutide control group (WT + Semaglutide) and Semaglutide treatment group (3xTg + Semaglutide). Semaglutide (25 nmol/kg) or PBS was administered intraperitoneally once every two days for 30 days, followed by behavioral and molecular experiments. The results show that Semaglutide can improve working memory and spatial reference memory of 3xTg-AD mice, promote the release of anti-inflammatory factors and inhibit the production of pro-inflammatory factors in the cortex and hippocampus, and reduce Aβ deposition in the hippocampal CA1 region of 3xTg mice. Semaglutide can inhibit the apoptosis of BV2 cells induced by Aβ1-42 in a dose-dependent manner and promote the transformation of microglia from M1 to M2, thereby exerting anti-inflammatory and neuroprotective effects. Therefore, we speculate that Semaglutide shows an anti-inflammatory effect by promoting the transformation of microglia from M1 to M2 type in the brain of 3xTg mice, and thus exerts a neuroprotective effect.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"563 ","pages":"Pages 222-234"},"PeriodicalIF":2.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central treatment of neuropeptide-S attenuates cognitive dysfunction and hippocampal synaptic plasticity impairment by increasing CaMKII/GluR1 in hemiparkinsonian rats 通过增加CaMKII/GluR1，中枢治疗神经肽-S可减轻半帕金森大鼠的认知功能障碍和海马突触可塑性损伤。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-14 DOI: 10.1016/j.neuroscience.2024.11.021

Osman Sinen , Ayşegül Gemici Sinen , Narin Derin

{"title":"Central treatment of neuropeptide-S attenuates cognitive dysfunction and hippocampal synaptic plasticity impairment by increasing CaMKII/GluR1 in hemiparkinsonian rats","authors":"Osman Sinen , Ayşegül Gemici Sinen , Narin Derin","doi":"10.1016/j.neuroscience.2024.11.021","DOIUrl":"10.1016/j.neuroscience.2024.11.021","url":null,"abstract":"<div><div>Neuropeptide-S (NPS) has been demonstrated to mitigate learning and memory deficits in experimental models of Parkinson’s Disease (PD). Despite this, the precise mechanisms through which NPS exerts its influence on cognitive functions remain to be fully unknown. This study aims to elucidate the effects of central administration of NPS on learning and memory deficits associated with an experimental rat hemiparkinsonian model, examining both electrophysiological and molecular parameters. The hemiparkinsonian model was established via stereotactic injection of 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle. Central NPS (1 nmol, icv) was administered into the lateral ventricle via a cannula for seven consecutive days following the 6-OHDA lesion. The Morris water maze and object recognition tests were used to evaluate the rat’s learning and memory abilities. Long-term potentiation (LTP) recordings were conducted to assess hippocampal synaptic plasticity. Immunohistochemistry was employed to determine the expression levels of phosphorylated CaMKII (pCaMKII), GluR1, and GluR2 in the hippocampus. The 6-OHDA-induced decline in cognitive performance was significantly (p < 0.05) improved in rats that received central NPS. In 6-OHDA-lesioned rats, NPS treatment significantly (p < 0.05) enhanced the amplitude of LTP at the dentate gyrus/perforant path synapses. Furthermore, NPS significantly (p < 0.05) increased the number of pCaMKII and GluR1 immunoreactive cells in the hippocampus, which had been diminished due to 6-OHDA, except for GluR2 levels. These findings provide insight into the mechanisms by which central NPS administration enhances cognitive functions in an experimental model of PD, highlighting its potential therapeutic benefits for addressing cognitive deficits in PD.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 194-201"},"PeriodicalIF":2.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary interventions in mitigating the impact of environmental pollutants on Alzheimer’s disease – A review 减轻环境污染物对阿尔茨海默病影响的膳食干预--综述。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-13 DOI: 10.1016/j.neuroscience.2024.11.020

Pratima Khandayataray , Meesala Krishna Murthy

{"title":"Dietary interventions in mitigating the impact of environmental pollutants on Alzheimer’s disease – A review","authors":"Pratima Khandayataray , Meesala Krishna Murthy","doi":"10.1016/j.neuroscience.2024.11.020","DOIUrl":"10.1016/j.neuroscience.2024.11.020","url":null,"abstract":"<div><div>Numerous studies linking environmental pollutants to oxidative stress, inflammation, and neurotoxicity have assigned pollutants to several neurodegenerative disorders, including Alzheimer’s disease (AD). Heavy metals, pesticides, air pollutants, and endocrine disruptor chemicals have been shown to play important roles in AD development, with some traditional functions in amyloid-β formation, tau kinase action, and neuronal degeneration. However, pharmacological management and supplementation have resulted in limited improvement. This raises the interesting possibility that activities usually considered preventive, including diet, exercise, or mental activity, might be more similar to treatment or therapy for AD. This review focuses on the effects of diet on the effects of environmental pollutants on AD. One of the primary issues addressed in this review is a group of specific diets, including the Mediterranean diet (MeDi), Dietary Approaches to Stop Hypertension (DASH), and Mediterranean-DASH intervention for Neurodegenerative Delay (MIND), which prevent exposure to these toxins. Such diets have been proven to decrease oxidative stress and inflammation, which are unfavorable for neuronal growth. Furthermore, they contribute to positive changes in the composition of the human gut microbiota and thus encourage interactions in the Gut-Brain Axis, reducing inflammation caused by pollutants. This review emphasizes a multi-professional approach with reference to nutritional activities that would lower the neurotoxic load in populations with a high level of exposure to pollutants. Future studies focusing on diet and environment association plans may help identify preventive measures aimed at enhancing current disease deceleration.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"563 ","pages":"Pages 148-166"},"PeriodicalIF":2.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between initial motor variability and learning and adaptive ability. A systematic review. 初始运动变异性与学习和适应能力之间的关系。系统综述。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-13 DOI: 10.1016/j.neuroscience.2024.10.052

Miguel López-Fernández, Rafael Sabido, Carla Caballero, Francisco J Moreno

{"title":"Relationship between initial motor variability and learning and adaptive ability. A systematic review.","authors":"Miguel López-Fernández, Rafael Sabido, Carla Caballero, Francisco J Moreno","doi":"10.1016/j.neuroscience.2024.10.052","DOIUrl":"https://doi.org/10.1016/j.neuroscience.2024.10.052","url":null,"abstract":"<p><p>Motor variability is an intrinsic feature of human beings that has been associated with the ability for learning and adaptation to specific tasks. The purpose of this review is to examine whether there is a possible direct relationship between individuals' initial variability, in both amount and structure variability, in their ability for learning and adaptation in motor tasks. Eighteen articles examined the relationship between initial motor variability and the ability for learning and adaptation. Twelve found a direct relationship. In reward learning task greater amount variability was associated with greater improvement learning, however, this association was not observed with structure variability. While in error learning task associations were reported with both greater amount variability and more complexity structure. Nevertheless, bias in initial performance related to the amount of variability was found, so the structure of initial variability seems to be a better indicator of improvement in this type of task. Further research is needed for further research to better understand the potential relationship between initial motor variability and the ability for learning and adaptation in motor tasks.</p>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dopamine-D2-agonist targets mitochondrial dysfunction via diminishing Drp1 mediated fission and normalizing PGC1-α/SIRT3 pathways in a rodent model of Subarachnoid Haemorrhage 在蛛网膜下腔出血的啮齿动物模型中，多巴胺-D2-受体激动剂通过减少 Drp1 介导的裂变和使 PGC1-α/SIRT3 通路正常化来靶向线粒体功能障碍。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-13 DOI: 10.1016/j.neuroscience.2024.11.028

Ahmed Shaney Rehman , Pravir Kumar , Suhel Parvez

{"title":"Dopamine-D2-agonist targets mitochondrial dysfunction via diminishing Drp1 mediated fission and normalizing PGC1-α/SIRT3 pathways in a rodent model of Subarachnoid Haemorrhage","authors":"Ahmed Shaney Rehman , Pravir Kumar , Suhel Parvez","doi":"10.1016/j.neuroscience.2024.11.028","DOIUrl":"10.1016/j.neuroscience.2024.11.028","url":null,"abstract":"<div><div>The adverse impact of disturbmitochondrialbiogenesis onearly brain injury (EBI) following subarachnoid haemorrhage (SAH) has been broadly recognized and is closely associated with oxidative stress and neuronal apoptosis. Previous studies have indicated the therapeutic potential of Ropinirole, a dopamine D2 agonist, in Ischemic Stroke. However, there is a lack of evidence regarding the ability of Ropinirole to enhance mitochondrial biogenesis and quality control after subarachnoid haemorrhage. The objective of this study is to investigate the effects of Ropinirole specific doses (10 & 20 mg/kg b. wt.) on mitochondria dysfunction in endovascular perforation SAH model in male Wistar rat. An endovascular perforation model was established using male Wistar rats that had sustained SAH injury. After the SAH injury, SAH grading on blood clot, Nissl staining, and neurobehavioral assessment were used to determine the severity. ROS and MMP, which are indicators of oxidative stress, were examined using flow cytometry. The findings demonstrated that the use of Ropinirole improved neurobehavioral outcomes, decreased brain edema, and reduced oxidative stress and mitochondrial based apoptosis. Further research showed that, Ropinirole therapy inhibit Drp1-mediated fission by accelerating the activity of fusion protein Mfn2/OPA1 along with regulating the translocation of PGC1-α and SIRT3 through restricting cytochrome <em>C</em> inside mitochondria to maintain mitochondrial metabolism. Ropinirole exerted neuroprotective effects by improving mitochondrial activity in a PGC1-α/SIRT3-dependent way via regulating Drp1 mediated fission. The effective treatment for SAH-induced EBI may involve increasing biogenesis and inhibiting excessive mitochondrial fission with Ropinirole.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 60-78"},"PeriodicalIF":2.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pretreatment with tetramethylpyrazine alleviated the impairment of learning and memory induced by sevoflurane exposure in neonatal rats. 四甲基吡嗪的预处理减轻了七氟醚暴露对新生大鼠学习和记忆的损害。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-12 DOI: 10.1016/j.neuroscience.2024.11.013

Kui Wang, Haidong Wei, Liufei Yang, Shuyue Zhang, Yiqin Cheng, Chen Li, Pengyu Jia, Yuanyuan Zhang, Yan Zhang, Pei Fan, Ning Wang, Haixia Lu, Xinlin Chen, Yong Liu, Pengbo Zhang

{"title":"Pretreatment with tetramethylpyrazine alleviated the impairment of learning and memory induced by sevoflurane exposure in neonatal rats.","authors":"Kui Wang, Haidong Wei, Liufei Yang, Shuyue Zhang, Yiqin Cheng, Chen Li, Pengyu Jia, Yuanyuan Zhang, Yan Zhang, Pei Fan, Ning Wang, Haixia Lu, Xinlin Chen, Yong Liu, Pengbo Zhang","doi":"10.1016/j.neuroscience.2024.11.013","DOIUrl":"https://doi.org/10.1016/j.neuroscience.2024.11.013","url":null,"abstract":"<p><p>Sevoflurane impairs learning and memory of the developing brain. However, strategies to mitigate these detrimental effects have been scarce. Herein, we investigated whether tetramethylpyrazine could alleviate the impairment of learning and memory and its underlying mechanisim in sevoflurane-exposed neonatal rats. Postnatal 7-day Sprague-Dawley (SD) rats or primary hippocampal neurons were pretreated with tetramethylpyrazine and then exposed to sevoflurane. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and lactate dehydrogenase (LDH) assays were used to detect neuronal injury. Learning and memory function were evaluated by novel object recognition and Morris water maze tests. Long-term potentiation (LTP) was recorded to evaluate synaptic plasticity electrophysiologically in the hippocampal slices. Golgi-Cox staining or PSD95 immunochemistry was used to detect the morphology of dendritic spines. Western blotting was employed to assess the expressions of cleaved Caspase-3, PSD95, NMDAR1, NMDAR2A and NMDAR2B in the hippocampus or cultured neurons. It was found that neonatal exposure of sevoflurane impaired learning and memory, increased neuronal apoptosis, altered the morphology of dendritic spine, upregulated the expressions of NMDAR2A and PSD95, and induced LTP deficits. Pretreatment with tetramethylpyrazine not only alleviated impairment of learning and memory, but also improved sevoflurane-induced changes in neuronal damage, dendritic spine morphology, NMDAR2A and PSD95 expressions, as well as LTP. These findings indicated that pretreatment with tetramethylpyrazine alleviated the impairment of learning and memory induced by sevoflurane through improvement of hippocampal synaptic plasticity in neonatal rats.</p>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma fibroblast growth factor 21 and risk of cognitive impairment among patients with ischemic stroke 血浆成纤维细胞生长因子 21 与缺血性中风患者认知障碍的风险。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-12 DOI: 10.1016/j.neuroscience.2024.11.012

Xiaowei Zheng , Zhengbao Zhu , Chongke Zhong , Daoxia Guo , Xiaoqing Bu , Hao Peng , Tan Xu , Yonghong Zhang

{"title":"Plasma fibroblast growth factor 21 and risk of cognitive impairment among patients with ischemic stroke","authors":"Xiaowei Zheng , Zhengbao Zhu , Chongke Zhong , Daoxia Guo , Xiaoqing Bu , Hao Peng , Tan Xu , Yonghong Zhang","doi":"10.1016/j.neuroscience.2024.11.012","DOIUrl":"10.1016/j.neuroscience.2024.11.012","url":null,"abstract":"<div><h3>Background and aims</h3><div>Previous study reported that plasma fibroblast growth factor 21 (FGF-21) was associated with poor prognosis in patients with ischemic stroke. The purpose of present study was to prospectively investigate the relationship between plasma FGF-21 and post-stroke cognitive impairment (PSCI).</div></div><div><h3>Methods</h3><div>A total of 600 patients from 7 hospitals were included in this study and plasma FGF-21 levels were examined for all the participants. Cognitive impairment was evaluated using Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) at 3 months after ischemic stroke onset.</div></div><div><h3>Results</h3><div>323(53.8 %) or 419(69.8 %) participants had PSCI according to MMSE or MoCA at 3 months, respectively. After adjustment for age, National Institutes of Health stroke score, education, and other covariates, the odds ratio of PSCI defined by MMSE and MoCA for the highest vs lowest quartile of plasma FGF-21 was 1.77(1.05–2.98) and 2.40(1.35–4.29), respectively. Multiple-adjusted spline regression model showed a linear association between FGF-21 levels and PSCI (all <em>P</em> < 0.005 for linearity). Subgroup analyses further confirmed these results.</div></div><div><h3>Conclusion</h3><div>Elevated plasma FGF-21 level was associated with PSCI at 3 months after stroke independently of established conventional risk factors, suggesting that plasma FGF-21 may have potential prognostic value in risk stratification of PSCI.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"563 ","pages":"Pages 129-135"},"PeriodicalIF":2.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0