Nature Reviews Molecular Cell Biology最新文献

Single-cell research in Latin America and the Caribbean builds genomics datasets for equitable AI-powered precision medicine. 拉丁美洲和加勒比地区的单细胞研究为公平的人工智能精准医疗建立了基因组学数据集。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-10-09 DOI: 10.1038/s41580-025-00913-5

Vinicius Maracaja-Coutinho,Helder I Nakaya

引用次数: 0

Elucidating the coordination of RNA processing using short-read and long-read RNA-sequencing methods. 利用短读和长读RNA测序方法阐明RNA加工的协调。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-10-06 DOI: 10.1038/s41580-025-00895-4

Carlos Alfonso-Gonzalez,Valérie Hilgers

{"title":"Elucidating the coordination of RNA processing using short-read and long-read RNA-sequencing methods.","authors":"Carlos Alfonso-Gonzalez,Valérie Hilgers","doi":"10.1038/s41580-025-00895-4","DOIUrl":"https://doi.org/10.1038/s41580-025-00895-4","url":null,"abstract":"The maturation of mRNAs is crucial for gene regulation and proteome diversification. Transcripts are processed co-transcriptionally through a complex interplay of mechanisms that involve numerous protein machineries. In eukaryotes, most genes undergo alternative RNA processing through the context-dependent use of transcription start sites (TSSs), splice sites and polyadenylation sites. The accurate measurement of alternative TSS usage, alternative splicing and alternative polyadenylation has been enabled by short-read RNA-sequencing technologies. However, elucidating the timing, coordination and functional outcomes of alternative RNA processing is challenging, especially in vivo. The development of long-read sequencing (LRS) methodologies enables the characterization of various aspects of co-transcriptional RNA processing, each methodology providing unique perspectives and limitations. In this Review, we discuss recent advances in short-read sequencing and LRS technologies that measure transcripts in their nascent and mature state and at single-cell resolution and with whole-molecule read length in the case of LRS. We integrate new findings that functionally link alternative TSS, alternative splicing and alternative polyadenylation, with new implications for diseases such as cancer and neurodevelopmental and neurodegenerative disorders. Finally, we discuss insights gained using CRISPR tools into the coordination of RNA processing events.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"52 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SLAM passes the haematopoietic stem cell identity test. SLAM通过造血干细胞识别测试。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-10-02 DOI: 10.1038/s41580-025-00906-4

Christina Marie Termini

引用次数: 0

Stem cell sources of colon cancer in mice. 小鼠结肠癌的干细胞来源。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-10-01 DOI: 10.1038/s41580-025-00910-8

Kim Baumann

引用次数: 0

The advent of confocal laser scanning microscopy in biological research. 共聚焦激光扫描显微镜在生物学研究中的应用。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-10-01 DOI: 10.1038/s41580-025-00905-5

Sean Munro

引用次数: 0

Mechanisms, functions and therapeutic targeting of protein tyrosine phosphatases. 蛋白酪氨酸磷酸酶的机制、功能和治疗靶点。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-09-30 DOI: 10.1038/s41580-025-00882-9

Tony Tiganis,Nicholas K Tonks

引用次数: 0

Publisher Correction: Collective cell migration modes in development, tissue repair and cancer. 出版者更正：集体细胞迁移模式在发展，组织修复和癌症。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-09-30 DOI: 10.1038/s41580-025-00911-7

Kevin J Cheung,Sally Horne-Badovinac

引用次数: 0

Towards a unified framework for the function of endoplasmic reticulum exit sites. 迈向内质网出口位点功能的统一框架。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-09-29 DOI: 10.1038/s41580-025-00899-0

Hesso Farhan,Ishier Raote,Felix Campelo,Liang Ge,Koret Hirschberg,Alison Forrester,Giulia Zanetti,Jennifer Lippincott-Schwartz,José Carlos Pastor-Pareja,Franck Perez,Kota Saito,Vivek Malhotra

{"title":"Towards a unified framework for the function of endoplasmic reticulum exit sites.","authors":"Hesso Farhan,Ishier Raote,Felix Campelo,Liang Ge,Koret Hirschberg,Alison Forrester,Giulia Zanetti,Jennifer Lippincott-Schwartz,José Carlos Pastor-Pareja,Franck Perez,Kota Saito,Vivek Malhotra","doi":"10.1038/s41580-025-00899-0","DOIUrl":"https://doi.org/10.1038/s41580-025-00899-0","url":null,"abstract":"Endoplasmic reticulum exit sites (ERES) are specialized, ribosome-free ER subdomains that serve as dynamic portals for COPII-mediated export of proteins from the ER. Beyond their role in the secretory pathway, ERES are implicated in diverse processes, including autophagy and the maturation of lipid droplets, highlighting their functional plasticity. ERES integrate cargo load, membrane tension and spatial cues to remodel their architecture and function in real time. This Roadmap synthesizes our current knowledge on the biogenesis, structural diversity and regulatory logic of ERES. We highlight key unanswered questions in the field, particularly concerning how ERES integrate signals to coordinate protein trafficking under varying cellular states. Finally, we propose a multidisciplinary framework - leveraging advances in high-resolution imaging, synthetic reconstitution and computational modelling - to delineate the principles governing the function and plasticity of ERES. Understanding these mechanisms holds significant potential for developing targeted therapeutic strategies in diseases linked to trafficking dysfunction.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"6 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The emergence of MARUbe - a hybrid ADP-ribose-ubiquitin modification. MARUbe的出现-一种adp -核糖-泛素修饰的杂交。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-09-26 DOI: 10.1038/s41580-025-00903-7

Jonathan N Pruneda,Roko Žaja,Michael S Cohen,Karla L H Feijs-Žaja

引用次数: 0

Coordination of cardiogenesis in vivo and in vitro. 体内和体外心脏发生的协调性。

IF 112.7 1区生物学

Nature Reviews Molecular Cell Biology Pub Date : 2025-09-24 DOI: 10.1038/s41580-025-00878-5

Sasha Mendjan,Alison Deyett,Deborah Yelon

引用次数: 0