Natural Product Communications最新文献_第4页

Determination of Total Flavonoid Content and Phenolic Content, Antioxidant Assay, and Antiepileptic Activity of Achillea millefolium Extract 千叶水蛭提取物总黄酮、酚类含量测定、抗氧化试验及抗癫痫活性研究

4区医学

Natural Product Communications Pub Date : 2025-02-01 DOI: 10.1177/1934578x251319221

Sonia Hayat, Ghulam Abbas Miana, Madiha Kanwal, Zartab Ahsan, Muhammad Junaid Tariq

{"title":"Determination of Total Flavonoid Content and Phenolic Content, Antioxidant Assay, and Antiepileptic Activity of Achillea millefolium Extract","authors":"Sonia Hayat, Ghulam Abbas Miana, Madiha Kanwal, Zartab Ahsan, Muhammad Junaid Tariq","doi":"10.1177/1934578x251319221","DOIUrl":"https://doi.org/10.1177/1934578x251319221","url":null,"abstract":"Objectives This study aimed to evaluate the antioxidant and antiepileptic potential of the ethanolic extract of Achillea millefolium aerial parts. Antioxidant activity was measured using DPPH photometric assay, and flavonoid content was quantified via the aluminum chloride method. The extract's neuroprotective effect was assessed using the pentylenetetrazole (PTZ)-induced seizure model, along with immunohistochemical (IHC) analysis and hematoxylin & eosin (H&E) staining. Methods The antioxidant potential of the extract was determined through DPPH radical scavenging activity, while flavonoid content was assessed as quercetin equivalents (QE). The in vivo antiepileptic activity was evaluated in mice using the PTZ-induced seizure model. Key biomarkers of oxidative stress, including glutathione (GSH), glutathione-S-transferase (GST), catalase, and lipid peroxidation (LPO), were measured to evaluate the extract's ability to ameliorate PTZ-induced oxidative stress. Results The extract demonstrated notable antioxidant activity, with 72% scavenging activity observed at 900 µg/mL. Flavonoid content was determined to be 39.45 ± 1.84 µg QE/mg. In the PTZ-induced seizure model, Achillea millefolium extract at 400 mg/kg and 600 mg/kg significantly reduced neuroinflammatory markers and oxidative stress, as evidenced by decreased LPO and increased levels of GSH, GST, and catalase. IHC and H&E staining revealed neuroprotective effects, including reduced neuroinflammation and enhanced neuronal survival. Conclusion The ethanolic extract of Achillea millefolium exhibited strong antioxidant and neuroprotective properties, effectively mitigating PTZ-induced seizures, oxidative stress, and inflammatory responses. These findings suggest its potential as a natural alternative for epilepsy treatment.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Anti-Alcoholism Effects of Tengcha–Gegen Plant Solid Beverage on Acute Alcohol Intoxication in Mice 藤茶葛根植物固体饮料对小鼠急性酒精中毒的抗酒精中毒作用

4区医学

Natural Product Communications Pub Date : 2025-01-01 DOI: 10.1177/1934578x251313877

Jiangning Peng, Yuning Jin, Shunwang Li, Gang Li, Yuhao Zhang, Zhe Yan, Lin Tang, Yanni Xu

{"title":"The Anti-Alcoholism Effects of Tengcha–Gegen Plant Solid Beverage on Acute Alcohol Intoxication in Mice","authors":"Jiangning Peng, Yuning Jin, Shunwang Li, Gang Li, Yuhao Zhang, Zhe Yan, Lin Tang, Yanni Xu","doi":"10.1177/1934578x251313877","DOIUrl":"https://doi.org/10.1177/1934578x251313877","url":null,"abstract":"Aims Acute alcohol intoxication (AAI) caused by the intake of excessive alcohol is a harmful clinical condition and potentially life threatening. Therefore, there has been increasing attention on the development of anti-alcoholism products. In this study, we evaluated the therapeutic benefits of an anti-alcoholism product (Tengcha–Gegen Plant Solid Beverage, which contains Ampelopsis grossedentata , Pueraria Lobata , Hovenia dulcis Thunb , and turmeric ) in an AAI mouse model. Methods The AAI mouse model was established through alcohol gavage. Male Kunming mice were first given three doses of Tengcha–Gegen Plant Solid Beverage for 30 min and then gavaged with 52% (V/V) liquor (0.15 mL liquor/10 g body weight). The impact of Tengcha–Gegen Plant Solid Beverage on AAI was evaluated by the sober-up time, the plasma alcohol content, and the plasma and liver superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) contents or activities. Results The Tengcha–Gegen Plant Solid Beverage could shorten the sober-up time and decrease plasma alcohol levels at 0.2 and 0.4 g/kg body weight. The mechanistic studies showed that Tengcha–Gegen Plant Solid Beverage increased the plasma SOD and liver GSH levels but decreased the plasma MDA levels at the indicated doses. Conclusions This study confirmed that Tengcha–Gegen Plant Solid Beverage might have anti-alcoholism therapeutic potential and protect the liver against high oxidative stress induced by acute alcohol exposure in AAI mice.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebral Ischemia Increases the Distribution and Residence Time of 6-Hydroxykynurenic Acid in the Ischemic Cerebral Cortex of Mice 脑缺血增加6-羟基尿酸在小鼠缺血大脑皮层的分布和停留时间

4区医学

Natural Product Communications Pub Date : 2025-01-01 DOI: 10.1177/1934578x251315082

Zhuowei Shen, Yanhong Chen, Kui Zeng, Haihong Hu, Jie Pan, Mingcheng Xu, Kaifeng He, Fengting Ou, Lushan Yu, Su Zeng, Lu Chen

{"title":"Cerebral Ischemia Increases the Distribution and Residence Time of 6-Hydroxykynurenic Acid in the Ischemic Cerebral Cortex of Mice","authors":"Zhuowei Shen, Yanhong Chen, Kui Zeng, Haihong Hu, Jie Pan, Mingcheng Xu, Kaifeng He, Fengting Ou, Lushan Yu, Su Zeng, Lu Chen","doi":"10.1177/1934578x251315082","DOIUrl":"https://doi.org/10.1177/1934578x251315082","url":null,"abstract":"Background 6-hydroxykynurenic acid (6-HKA), isolated from Ginkgo biloba leaves, has demonstrated neurorestorative properties against cerebral ischemia in middle cerebral artery occlusion (MCAO) mice models. However, our previous studies showed that 6-HKA was difficult to penetrate the blood brain barrier (BBB) to exert its pharmacological effects in rats. Therefore, this study aims to investigate the pharmacokinetics and cerebral cortex distribution of 6-HKA in MCAO mice, to determine whether the ability of 6-HKA to cross the BBB is contingent upon barrier integrity. Methods This study established and validated the ultra-performance liquid chromatography tandem mass spectrometric (UPLC-MS/MS) methods for the quantification of 6-HKA in mouse plasma and brain, and investigated the pharmacokinetics and cerebral cortex distribution of 6-HKA in MCAO mice and sham-operated mice. Results This study demonstrates that the mean residence time (MRT 0−t ) of 6-HKA in the MCAO group was significantly prolonged compared to the sham-operated group, and there was a higher distribution of 6-HKA in the ischemic cerebral cortex than in the contralateral normal cerebral cortex. Conclusion This study illustrated that in MCAO mice, the prolonged MRT 0−t of 6-HKA and the increased permeability of BBB made the accumulation of 6-HKA in the ischemic cerebral cortex, thereby enabling 6-HKA to exert neurorestorative effects.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating “Xinshuixiao” Serum's Impact on dDAVP-Induced AQP2 Expression and Mechanisms in IMCD3 Cells 心水消血清对ddavp诱导的IMCD3细胞AQP2表达的影响及机制研究

4区医学

Natural Product Communications Pub Date : 2025-01-01 DOI: 10.1177/1934578x241289213

Qian Wei, Yang Li, Yanxin Wang, Cheng Luan, Tianyang Zhang

{"title":"Investigating “Xinshuixiao” Serum's Impact on dDAVP-Induced AQP2 Expression and Mechanisms in IMCD3 Cells","authors":"Qian Wei, Yang Li, Yanxin Wang, Cheng Luan, Tianyang Zhang","doi":"10.1177/1934578x241289213","DOIUrl":"https://doi.org/10.1177/1934578x241289213","url":null,"abstract":"Objective To investigate the impact of “Xinshuixiao” drug-containing serum on dDAVP-induced AQP2 expression in IMCD3 cells and to preliminarily explore its underlying mechanisms. Methods Rats were orally administered with “Xinshuixiao granules” containing medicinal compounds. The CCK-8 assay was employed to determine the appropriate drug serum concentration and its effect on IMCD3 cell viability. Additionally, we assessed the influence of “Xinshuixiao” drug-containing serum on IMCD3 cell activity following dDAVP intervention. Cells were divided into blank, model, low, medium, and high-dose “Xinshuixiao” drug serum groups, as well as a Tolvaptan group. After 24 h of drug treatment, ELISA was used to measure the levels of arginine vasopressin (AVP), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) in the cell supernatant. Western blotting was employed to evaluate AQP2 protein expression, and real-time polymerase chain reaction (Real-time PCR) was used to assess AQP2 mRNA expression levels. Results In comparison to the blank group, the model group exhibited elevated expression of AVP, cAMP, PKA ( P<0.05), as well as increased AQP2 mRNA and protein expression ( P<0.05). When compared to the model group, all treatment groups displayed varying degrees of reductions in AVP, cAMP, and PKA expression ( P<0.05), along with decreased AQP2 mRNA and protein expression, with statistically significant differences ( P<0.05). Conclusion “Xinshuixiao” drug-containing serum exerts an inhibitory effect on the overexpression of AQP2 induced by dDAVP in IMCD3 cells, which may be associated with its suppression of the cAMP/PKA signaling pathway.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Evaluation of EGCG against Atopic Dermatitis: An in Vivo and in Silico Study EGCG抗特应性皮炎的机制评价：体内和计算机研究

4区医学

Natural Product Communications Pub Date : 2024-12-01 DOI: 10.1177/1934578x241304962

Liu Tang, Tianshuai Wang, Ruzhen Peng, Jingyi Guo, Liping Li, Benhong Zhou, Guangwen Luo, Shasha Fang, Xiao Jiang

{"title":"Mechanistic Evaluation of EGCG against Atopic Dermatitis: An in Vivo and in Silico Study","authors":"Liu Tang, Tianshuai Wang, Ruzhen Peng, Jingyi Guo, Liping Li, Benhong Zhou, Guangwen Luo, Shasha Fang, Xiao Jiang","doi":"10.1177/1934578x241304962","DOIUrl":"https://doi.org/10.1177/1934578x241304962","url":null,"abstract":"Background: Atopic dermatitis, often referred to as AD, is a common chronic and relapsing skin disease with few treatment options. Epigallocatechin 3-gallate (EGCG), the most abundant catechin derived from green tea, has gained widespread attention due to its excellent anti-inflammatory and anti-oxidative properties. This research aims to investigate the effect and mechanism of EGCG in alleviating AD by integrative approach of an in vivo AD mouse model, network pharmacology and molecular docking. Materials and Methods: EGCG was administered by intragastric administration at the dose of 100 mg/kg, once daily for 14 consecutive days to AD mice, a model established by repeated application of 2, 4-dinitrofluorobenzene (DNFB) to the mouse dorsal skin. Results: Macroscopical and histopathological examination showed that oral administration with EGCG could markedly relieve AD-like skin phenotypes, as evidenced by a substantial reduction in severity score, serum immunoglobulin E (IgE) level, epidermal thickness, and the infiltration of inflammatory cells and mast cells into the dermal layers. Mechanistically, the network pharmacology analysis indicated that the protective effects of EGCG against AD were mainly related to the regulation of AGE-RAGE signaling pathway in diabetic complications, Th17 cell differentiation, IL-17 signaling pathway, NF-κB signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway, etc. In silico molecular docking demonstrated the high binding affinity of EGCG to the core targets such as ABCB1, MMP2, MMP9, MAPK14 and STAT1. Furthermore, western blotting (WB) results showed that EGCG obviously downregulated the expression of RAGE and STAT1, thereby regulating the AGE-RAGE signaling pathway to improve AD. Conclusions: These findings indicated that EGCG can be a promising therapeutic candidate for AD.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147918406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Danshensu and Notoginsenoside R1 Combination Alleviates Obesity in Mice Via Modification of Gut Microbiota and Short-Chain Fatty Acids 丹参素与三七皂苷R1联合应用通过改变肠道菌群和短链脂肪酸减轻小鼠肥胖

4区医学

Natural Product Communications Pub Date : 2024-12-01 DOI: 10.1177/1934578x241293024

Hui‐Juan Tao, Wenxiao Wang, Hui Cao, Ning Tong, Li Ma, Jie Shen, Qiao Zhang, Yuping Tang, Shi‐Jun Yue

{"title":"Danshensu and Notoginsenoside R1 Combination Alleviates Obesity in Mice Via Modification of Gut Microbiota and Short-Chain Fatty Acids","authors":"Hui‐Juan Tao, Wenxiao Wang, Hui Cao, Ning Tong, Li Ma, Jie Shen, Qiao Zhang, Yuping Tang, Shi‐Jun Yue","doi":"10.1177/1934578x241293024","DOIUrl":"https://doi.org/10.1177/1934578x241293024","url":null,"abstract":"Objectives The current study evaluated the protective mechanism of Danshensu and Notoginsenoside R1 combination (DR1) on obesity from the perspective of gut microbiota and short-chain fatty acids (SCFAs). Methods Male C57BL/6J mice were fed either a normal diet or a high-fat diet (HFD). DR1 (60 mg/kg) was administered to both normal and HFD-induced obese mice for 12 weeks. The obese phenotype and pharmacodynamics effects were assessed through hematoxylin-eosin staining of epididymal fat, liver, small intestine, and colon tissues, as well as serum biochemical detection. Moreover, 16S ribosomal ribonucleic acid (16S rRNA) sequencing was employed to profile the taxonomy and function of gut microbiota, while SCFAs were quantified using ultra-performance liquid chromatography coupled with mass spectrometry methods. Results Compared to HFD group, the tissue lesions were significantly alleviated by DR1 treatment in obese mice, accompanied by the significant reduction of average body, epididymal fat and liver weights, as well as the normalization of insulin sensitivity and glucose homeostasis ( P < .05, P < .01 or P < .001). 16S rRNA sequencing analysis showed that DR1 treatment potently ameliorated HFD-induced dysbiosis of gut microbial composition and function. Notably, DR1 reversed the ratio of Firmicutes/ Bacteroidetes to normality. Moreover, DR1 improved intestinal barrier structures and enhanced SCFAs concentrations in the caecal contents (especially propionate and butyrate). Conclusion These results suggested that DR1 could reverse the gut microbial dysbiosis and increased SCFAs levels in HFD-induced obese mice, thereby ultimately supporting the anti-obesity value of DR1.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scopoletin as Key Component of a Novel Acaricidal Agent and Its Production Methodology 一种新型杀螨剂的关键成分东莨菪碱及其生产方法

4区医学

Natural Product Communications Pub Date : 2024-12-01 DOI: 10.1177/1934578x241306808

Guanxiong Chen, Yimeng Zhang, Yimeng Zhang, Xinyue Chen, Jingxing Yuan, Yongqiang Zhang, Yongqiang Zhang

{"title":"Scopoletin as Key Component of a Novel Acaricidal Agent and Its Production Methodology","authors":"Guanxiong Chen, Yimeng Zhang, Yimeng Zhang, Xinyue Chen, Jingxing Yuan, Yongqiang Zhang, Yongqiang Zhang","doi":"10.1177/1934578x241306808","DOIUrl":"https://doi.org/10.1177/1934578x241306808","url":null,"abstract":"Background: A large number of available substances were retained in artemisinin residual materials, and they can be extracted through further separation and purification for further utilization. Methods: Purification and bioguided separation, including silica gel column chromatography (SGCC) and high-pressure liquid chromatography (HPLC), were utilized to isolate scopoletin, an acaricidal compound, from residual materials of Artemisia annua after extraction of artemisinin (RMAAEA). A methanol crude extract was prepared and subjected to chromatographic purification. Results: A total of 20 groups were obtained. The 17th, 8th, and 18th groups showed stronger acaricidal activity against Tetranychus cinnabarinus than other groups. The 17th group was isolated by further HPLC and divided into three parts: 17-1 (16.21%, w/w), 17-2 (5.31%, w/w), and 17-3 (77.64%, w/w). The LC 50 value at 48 h after treatment against T. cinnabarinus was 181.3, 701.1, and 101.5 mg·L −1 . Conclusion: The combined elements of 17-3 after purification was determined as scopoletin. Its industrial process with 900 kg of residual materials finally obtained 1.23 kg of pure scopoletin.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yougui Pills Alleviates Diminished Ovarian Reserve Through Regulating Oxidative Stress and Apoptosis in Rats 右桂丸通过调节氧化应激和细胞凋亡减轻大鼠卵巢储备功能减退

4区医学

Natural Product Communications Pub Date : 2024-11-01 DOI: 10.1177/1934578x241296409

Beibei Jiao, Tian Li, Beibei Zhou, Si Chen, Yue Chen, Yan Lu, Jianmin Liu, Weibo Zhao, Jian Zhang, Pei-Juan Wang

{"title":"Yougui Pills Alleviates Diminished Ovarian Reserve Through Regulating Oxidative Stress and Apoptosis in Rats","authors":"Beibei Jiao, Tian Li, Beibei Zhou, Si Chen, Yue Chen, Yan Lu, Jianmin Liu, Weibo Zhao, Jian Zhang, Pei-Juan Wang","doi":"10.1177/1934578x241296409","DOIUrl":"https://doi.org/10.1177/1934578x241296409","url":null,"abstract":"Objective Yougui pills (YGP) have been used clinically to treat diminished ovarian reserve (DOR), but, whether YGP could have a palliative effect in the DOR rat model and the underlying mechanisms have not been clearly elucidated. This study aims to investigate the therapeutic effect and potential mechanisms of YGP in rats with cortisol-induced DOR. Methods The cortisol-induced DOR model was used to investigate the protective effects and mechanism of YGP on DOR. The DOR model was established by intramuscular injection of 25 mg/kg cortisol for 28 consecutive days. Meanwhile, the rats were weighed and administered intragastrically YGP or progynova. To evaluate the mitigating effect of YGP on DOR, pharmacodynamic indicators were assessed including body weight, estrous cycle, H&E staining, and serum levels of AMH, FSH, LH, and cortisol. Oxidative stress (OS) and apoptosis pathways were further validated using Western blotting analysis. Result YGP significantly ameliorated the symptoms of DOR. Administration of YGP improved the ovarian index, estrous cycle, and body weight. After treatment with YGP, cortisol, FSH, and LH levels declined and AMH levels elevated. YGP increased the number of primary, secondary, and antral follicles, but decreased the number of atretic follicles. Mechanically, YGPtreated cortisol-induced DOR via the Keap1/Nrf2 signaling pathway which regulates OS. YGP also reversed the aberrant expression of apoptosis-related proteins Caspase-3, Bcl-2, and Bax. Conclusion YGP could reduce ovarian OS and apoptosis in cortisol-induced rats via regulating the Keap1/Nrf2 signaling pathway, thereby enhancing ovarian reserve function.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Mechanism of Compound Zeqi Granule in Treating Psoriasis vulgaris Based on Network Pharmacology and Experimental Verification 复方泽气颗粒治疗寻常型银屑病机理的网络药理学研究及实验验证

4区医学

Natural Product Communications Pub Date : 2024-11-01 DOI: 10.1177/1934578x241300924

Min Wu, Tongtong Dong, Ting Zhang, Jiuxiang Wang, Taofeng Liu, Changzhong Wang

{"title":"Study on the Mechanism of Compound Zeqi Granule in Treating Psoriasis vulgaris Based on Network Pharmacology and Experimental Verification","authors":"Min Wu, Tongtong Dong, Ting Zhang, Jiuxiang Wang, Taofeng Liu, Changzhong Wang","doi":"10.1177/1934578x241300924","DOIUrl":"https://doi.org/10.1177/1934578x241300924","url":null,"abstract":"Background This study used a holistic network pharmacological approach to clarify the mechanism of Compound Zeqi Granule (CZG) in treating psoriasis vulgaris (PV). Methods The potential bioactive compounds and corresponding targets of CZG were collected and searched via the TCMSP database. Disease targets of PV were obtained from the GeneCards database. The targets of CZG and PV were standardized by the UniProt database. GO and KEGG enrichment analyses were performed using the Metascape database. Then, molecular docking verification was conducted using the CB-Dock database. Animal experiments were conducted to validate the effects of CZG on PV. Results This approach identified 99 bioactive compounds, 579 potential drug targets, 822 PV-related targets, and 108 potential target proteins. The biological processes were primarily related to regulation of defense response, regulation of inflammatory responses, and cellular response to lipid. The targets of CZG for treating PV were significantly associated with 20 pathways including JAK-STAT signaling pathway. Animal experiments confirmed that CZG exerted its therapeutic effects on PV by promoting the expression of IL-10 and JUN, and inhibiting the activation of STAT1 and STAT3. Conclusion The present study provides evidence supporting the efficacy of CZG for the treatment of PV through a multi-compound, multi-target and multi-pathway approach and lays a theoretical foundation for further experimental research in this field.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shen Gu An Capsule Inhibition of Postmenopausal Osteoporosis in Ovariectomized Mice Using Network Pharmacology-Based Mechanism Prediction and Pharmacological Validation 参骨安胶囊对去卵巢小鼠绝经后骨质疏松症的抑制作用：基于网络药理学的机制预测及药理学验证

4区医学

Natural Product Communications Pub Date : 2024-11-01 DOI: 10.1177/1934578x241297039

Mingde Du, Shiguo Yuan, Jiayang Wang, Qiangqiang Zhao, Baofei Ye, Haiyan Wu, Liangliang Xu, Yu Hou

{"title":"Shen Gu An Capsule Inhibition of Postmenopausal Osteoporosis in Ovariectomized Mice Using Network Pharmacology-Based Mechanism Prediction and Pharmacological Validation","authors":"Mingde Du, Shiguo Yuan, Jiayang Wang, Qiangqiang Zhao, Baofei Ye, Haiyan Wu, Liangliang Xu, Yu Hou","doi":"10.1177/1934578x241297039","DOIUrl":"https://doi.org/10.1177/1934578x241297039","url":null,"abstract":"Background Postmenopausal osteoporosis (PMOP) is characterized by low bone mass and increased bone fragility in postmenopausal women. Shen Gu An capsule (SGA) has been clinically used to treat bone diseases, especially PMOP with kidney Yang deficiency. Objectives We aimed to study the effect of SGA on the treatment of PMOP. Methodology The key candidate targets and pathways of SGA for treating PMOP were predicted by Network pharmacology analysis. Then, in vivo validation using bilaterally ovariectomized C57BL/6 mice was performed, and the bone quality was analyzed by Micro-CT and histological analysis. Results In this study, we obtained 113 active compounds and 82 targets via the database of Traditional Chinese Medicine Systems Pharmacology. Subsequently, compound-target and protein−protein interaction networks were constructed. Then GO and KEGG analysis revealed that NF-κB signaling pathway might be the underlying mechanism as its well-known function in osteoclastogenesis. Furthermore, the in vivo experiments using ovariectomized mice demonstrated that SGA could decrease the number of osteoclasts and preserve bone mass through inhibiting the NF-κB signaling pathway. Conclusions SGA could prevent postmenopausal osteoporosis in ovariectomized mice through inhibiting NF-κB signaling pathway. The finding of this study provides more evidence for the clinical application of SGA to treat osteoporosis.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"19 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0