Molecular Plant-microbe Interactions最新文献_第2页

Early Activation of RNAi Reveals Genomic Regions of Grapevine Red Blotch Virus Targeted for Silencing in Grapevine. 早期激活的RNAi揭示了葡萄红斑病病毒沉默的目标基因组区域。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-05-07 DOI: 10.1094/MPMI-04-25-0038-R

Christian Mandelli, Laurent G Deluc

{"title":"Early Activation of RNAi Reveals Genomic Regions of Grapevine Red Blotch Virus Targeted for Silencing in Grapevine.","authors":"Christian Mandelli, Laurent G Deluc","doi":"10.1094/MPMI-04-25-0038-R","DOIUrl":"https://doi.org/10.1094/MPMI-04-25-0038-R","url":null,"abstract":"Grapevine red blotch virus (GRBV), a member of the Geminiviridae family that causes reduced fruit quality and yield, is an emerging challenge for the wine industry. Viticultural practices and pest management have been largely ineffective at mitigating the impacts of GRBV, necessitating alternative control strategies. Here, we investigated the early activation of RNA interference (RNAi) in GRBV-infected grapevines and, through small RNA sequencing, identified nine genomic virus-derived small-interfering RNA (vsiRNA)-producing regions referred to as hotspots (HS). Subsequent analyses revealed that these HS were primarily involved in producing 24-nt vsiRNA species associated with transcriptional gene silencing towards later stages of infection. Double-stranded RNA (dsRNA) molecules derived from these HS were administered to GRBV-infected plants via root soaking, significantly (p < 0.05) reducing viral gene expression in leaves and petioles for up to one month. Ultimately, we assessed the potential of viral mutation within these HS, identified areas of higher mutational entropy, and found that most HS locations are within viral regions with lower probabilities of mutation events. These findings provide the basis for future research to characterize the role of small RNA (sRNA)-induced silencing mechanisms in grapevine-GRBV interactions and their potential translation for field-based technology like RNAi biopesticide to manage red blotch disease.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular Responses in the Pigeonpea Wild Relative Cajanus platycarpus to Helicoverpa armigera Herbivory: The Role of Methionine Sulfoxide Reductase B1 (CpMSRB1) in Enhanced Defense. 野生种鸭嘴蝉对棉铃虫的细胞反应：蛋氨酸亚砜还原酶B1 （CpMSRB1）在增强防御中的作用

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-05-05 DOI: 10.1094/MPMI-11-24-0149-R

Maniraj Rathinam, Narasimham Dokka, Kameshwaran Senthil, Shivangi Mahawar, Shaily Tyagi, Dineshkumar Rengarajan, Preethi Vijayaraghavareddy, Yuvaraj Iyyappan, Basavaraj Y B, Sandeep Reddy, Vinutha T, Rama Prashat G, Subodh Kumar Sinha, Prasanta K Dash, Sheshshayee Sreeman, Manoj Majee, Rohini Sreevathsa

{"title":"Cellular Responses in the Pigeonpea Wild Relative Cajanus platycarpus to Helicoverpa armigera Herbivory: The Role of Methionine Sulfoxide Reductase B1 (CpMSRB1) in Enhanced Defense.","authors":"Maniraj Rathinam, Narasimham Dokka, Kameshwaran Senthil, Shivangi Mahawar, Shaily Tyagi, Dineshkumar Rengarajan, Preethi Vijayaraghavareddy, Yuvaraj Iyyappan, Basavaraj Y B, Sandeep Reddy, Vinutha T, Rama Prashat G, Subodh Kumar Sinha, Prasanta K Dash, Sheshshayee Sreeman, Manoj Majee, Rohini Sreevathsa","doi":"10.1094/MPMI-11-24-0149-R","DOIUrl":"https://doi.org/10.1094/MPMI-11-24-0149-R","url":null,"abstract":"Understanding key cellular mechanisms leading to improved defense against various stressors is essential for cultivating robust nutritious crops capable of flourishing in diverse environments. We present an in-depth characterization of the defense response in the pigeonpea wild relative, Cajanus platycarpus, to herbivory by pod borer, Helicoverpa armigera. To fight the attacking pest, C. platycarpus strategically activated non-enzymatic ROS scavengers and unleashed methionine sulfoxide reductases (MSRs) to safeguard the integrity of methionine residues. We unveiled for the first time physical interaction between CpMSRB1 and chorismate mutase (CpCM1.1), a pivotal player in the phenylpropanoid pathway (PP). This association fuelled the synthesis of phenylpropanoids and enhanced ROS scavenging crucial for repelling herbivores. Repairing CpCM1.1 also boosted salicylic acid (SA) production, coordinating defense signaling with jasmonic acid (JA). Additionally, heterologous expression of CpMSRB1 in tomato improved defense against herbivory by enhanced ROS scavenging and polyphenol production. This study demonstrates the role of CpMSRB1 to protect a major enzyme in the shikimate pathway, reinforcing defense against H. armigera.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleiotropic Phenotypes of the Tomato diageotropica Mutant Enable Resistance to Ralstonia solanacearum. 番茄嗜病突变体的多效表型使其能够抵抗茄枯病。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-25 DOI: 10.1094/MPMI-10-24-0123-R

Katherine Rivera-Zuluaga, Pratibha Choudhary, Sana Mohammad, Denise Caldwell, Rebecca Leuschen-Kohl, Pete E Pascuzzi, Anjali Iyer-Pascuzzi

{"title":"Pleiotropic Phenotypes of the Tomato diageotropica Mutant Enable Resistance to Ralstonia solanacearum.","authors":"Katherine Rivera-Zuluaga, Pratibha Choudhary, Sana Mohammad, Denise Caldwell, Rebecca Leuschen-Kohl, Pete E Pascuzzi, Anjali Iyer-Pascuzzi","doi":"10.1094/MPMI-10-24-0123-R","DOIUrl":"https://doi.org/10.1094/MPMI-10-24-0123-R","url":null,"abstract":"Quantitative disease resistance (QDR) is the most common form of disease resistance in crops, but is challenging to understand at the cellular level due to the involvement of many genes and biological processes. Ralstonia solanacearum, the causal agent of bacterial wilt disease, is a destructive plant pathogen of Solanaceous species that is best controlled by quantitatively resistant varieties, but few QDR genes are known. We previously found that a tomato auxin pathway mutant known as diageotropica (dgt) has enhanced resistance to R. solanacearum. Here we show that, like wild-type quantitatively resistant tomato plants, resistance in dgt is the result of multiple mechanisms. Mock-inoculated dgt roots have endogenously higher levels of the plant defense hormone salicylic acid (SA). However, the SA-deficient double mutant dgtNahG is still resistant to R. solanacearum, indicating that SA-independent pathways are also required for resistance. Scanning electron microscopy revealed that R. solanacearum colonization of root xylem is delayed in dgt. We found an increased number of lignified xylem cells and altered root vasculature anatomy in dgt, and dgt root length was not impacted by R. solanacearum treatment. Similar to the wilt-resistant wild-type tomato Hawaii7996, RNA-seq results suggested that dgt may tolerate R. solanacearum-induced water stress better than the wilt-susceptible parent. Thus, resistance in dgt is due to several pathways, including pre-activated SA defenses, physical barriers in the xylem, and an ability to tolerate water stress. The pleiotropic nature of this single mutation appears to mimic quantitative resistance.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Soilborne Fungus Verticillium longisporum and Its Interactions with the Brassicaceous Hosts. 土传真菌长孢黄萎病及其与十字花科寄主的相互作用。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-25 DOI: 10.1094/MPMI-03-25-0029-IRW

Vahideh Rafiei, Christina Dixelius, Georgios Tzelepis

{"title":"The Soilborne Fungus Verticillium longisporum and Its Interactions with the Brassicaceous Hosts.","authors":"Vahideh Rafiei, Christina Dixelius, Georgios Tzelepis","doi":"10.1094/MPMI-03-25-0029-IRW","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0029-IRW","url":null,"abstract":"Verticillium longisporum, a soilborne fungal species, is the causative agent of Verticillium stripe disease in Brassica species and represents a notable threat to agricultural production, particularly in regions where oilseed rape is a major crop, including Europe, North America, and Asia. The microsclerotia of this pathogen can persist in the soil for extended periods, with a potential lifespan of up to a decade, thereby posing a substantial challenge for the complete eradication of the pathogen from infested soil. The genome of V. longisporum is amphidiploid and resulted from the hybridization of V. dahliae (D genotypes) and an unidentified species (A1 genotype). At least three independent hybridization events are estimated to have occurred, resulting in three distinct lineages: A1/D1, A1/D2, and A1/D3. Genome sequence analysis revealed the presence of mating-type idiomorphs, putative cell wall-degrading enzymes, and effectors. However, due to the complexity of the genome, there is a paucity of research on the molecular interactions between V. longisporum and Brassica crops. This review summarizes the extant knowledge regarding the pathogenicity factors that V. longisporum deploys upon infection and the host immune responses against this attack, highlighting aspects that remain to be elucidated and the molecular tools available for studying this interaction. A better understanding of the molecular interactions in this pathosystem will contribute to developing more effective control measures against this disease in Brassica oilseed and cabbage crops.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SRF6 Is Necessary for the Perception of the Cell Wall Component TGA by Arabidopsis thaliana and Its Subsequent Immune Reaction. SRF6是拟南芥感知细胞壁成分TGA及其随后的免疫反应所必需的。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-23 DOI: 10.1094/MPMI-04-25-0036-R

Aparna Bhasin, Stephanie Chan, Ishal Dave, Leah Ritchie, G Adam Mott

{"title":"SRF6 Is Necessary for the Perception of the Cell Wall Component TGA by Arabidopsis thaliana and Its Subsequent Immune Reaction.","authors":"Aparna Bhasin, Stephanie Chan, Ishal Dave, Leah Ritchie, G Adam Mott","doi":"10.1094/MPMI-04-25-0036-R","DOIUrl":"https://doi.org/10.1094/MPMI-04-25-0036-R","url":null,"abstract":"Plants are sessile organisms and must accurately respond to a host variety of growth, developmental, and environmental signals throughout their life to maximize fitness. Plant cell surface receptor-like kinases are ideal for the perception of such signals and their transduction within the cell. The Strubbelig Receptor Family (SRF) is a group of leucine-rich repeat receptor-like kinases, several of which have unknown function. Here, we identify a role for SRF6 in the perception of cell wall damage and the activation of downstream immune responses. We show that SRF6 is necessary for proper immune responses following elicitation with a short-chain oligogalacturonic acid, including activation of defense genes and increased bacterial resistance. We also demonstrated the srf6 mutants are more sensitive to isoxaben treatment, suggesting enhanced cell wall integrity maintenance responses. These findings are compatible with the hypothesis that cell wall integrity maintenance responses are elevated when pattern-triggered immunity is compromised.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zymoseptoria tritici Show Local Differences in Within-Field Diversity and Effector Variation. 小麦酵母在田间多样性和效应变异方面存在局部差异。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-23 DOI: 10.1094/MPMI-01-25-0006-FI

Andrea Tobian Herreno, Pu Huang, Isabella Siepe, Remco Stam

{"title":"Zymoseptoria tritici Show Local Differences in Within-Field Diversity and Effector Variation.","authors":"Andrea Tobian Herreno, Pu Huang, Isabella Siepe, Remco Stam","doi":"10.1094/MPMI-01-25-0006-FI","DOIUrl":"https://doi.org/10.1094/MPMI-01-25-0006-FI","url":null,"abstract":"Zymoseptoria tritici is a cosmopolitan hemibiotrophic wheat pathogen with a high mutation rate and a mixed reproduction system, with sexual and asexual cycles occurring within the same disease cycle. This leads to challenges in traditional farming management. For successful integrated pest management, especially for surveys of new aggressive lineages, it is critical to understand population diversity in the field. We look at whole-genome sequence data for three datasets to differentiate within field diversity in fields of similar size: one dataset from a newly sampled field population from the United Kingdom and two publicly available datasets from fields from the United States and Switzerland. This survey of genetic variation allows us to describe in detail how variable the field populations are and gives insight into the dynamics of the disease in a snapshot per field. Inspection of population structure and diversity features, such as minor allele frequency distribution and clonality, show no within-field structure, the most abundant SNPs are present in low frequency, and European fields have higher clonality. Knowing that effectors play particularly important roles in (a)virulence, we specifically assess effector diversity characteristics. On a whole-genome scale, we can see separation of the populations at the regional scale, but we do not find such separation for the effectors. Moreover, we find that multiple effector haplotypes can be found interspersed within the field and even occur within what has been considered clonal isolates or isolates from a single lesion.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome and Cell Type Signature Analysis of Laser-Microdissected Syncytia Induced by the Cyst Nematode Heterodera schachtii in Arabidopsis Roots. 沙氏囊线虫诱导拟南芥根系激光显微解剖合胞体的转录组和细胞类型特征分析。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-22 DOI: 10.1094/MPMI-03-25-0024-R

Xunliang Liu, Melissa G Mitchum

{"title":"Transcriptome and Cell Type Signature Analysis of Laser-Microdissected Syncytia Induced by the Cyst Nematode Heterodera schachtii in Arabidopsis Roots.","authors":"Xunliang Liu, Melissa G Mitchum","doi":"10.1094/MPMI-03-25-0024-R","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0024-R","url":null,"abstract":"Cyst nematodes (CNs) establish a highly specialized feeding structure called a syncytium in host roots by secreting effectors into a selected host cell that reprogram host development programs. The selected host cell undergoes distinct morphological, physiological, and gene expression changes, resulting in the fusion of hundreds of cells to create a novel cell type that does not normally exist in the host. Here, we profiled the transcriptome of the syncytium induced by the beet cyst nematode (BCN) Heterodera schachtii in Arabidopsis roots using laser capture microdissection and RNA-sequencing. Aside from biological processes that are expected to be altered by nematode infection, we also found that genes annotated in nitrate and iron ion signaling and transport related biological processes are significantly overrepresented in genes that are down-regulated by BCN infection, suggesting these ions may play important roles in BCN infection. Comparing the syncytium transcriptome to that of various root cell types showed that it was overrepresented by genes that are enriched in cells marked by ATHB15, a member of HD-ZIP III transcription factor family that is highly expressed in the stem cell organizer of the root vasculature. These results suggest that the syncytium may partially adopt the molecular signature of a stem cell organizer, consistent with our previous hypothesis that BCN uses a stem cell organizer as an intermediate status for syncytium formation.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xanthomonas oryzae pv. oryzae Type-III Effector Tal9b Targets a Broadly Conserved Disease Susceptibility Locus to Promote Pathogenesis in Rice. 米黄单胞菌。oryzae iii型效应物Tal9b靶向广泛保守的疾病易感位点促进水稻发病

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-15 DOI: 10.1094/MPMI-10-24-0139-R

Gokulan C G, Sohini Deb, Namami Gaur, Apoorva Masade, Niranjan Gattu, Rennya P R, Nisha Sao, Donald James, Ramesh V Sonti, Hitendra K Patel

{"title":"Xanthomonas oryzae pv. oryzae Type-III Effector Tal9b Targets a Broadly Conserved Disease Susceptibility Locus to Promote Pathogenesis in Rice.","authors":"Gokulan C G, Sohini Deb, Namami Gaur, Apoorva Masade, Niranjan Gattu, Rennya P R, Nisha Sao, Donald James, Ramesh V Sonti, Hitendra K Patel","doi":"10.1094/MPMI-10-24-0139-R","DOIUrl":"https://doi.org/10.1094/MPMI-10-24-0139-R","url":null,"abstract":"Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice, translocates multiple Transcription Activator-Like Effectors (TALEs) into rice cells. The TALEs localize to the host cell nucleus, where they bind to the DNA in a sequence-specific manner and enhance gene expression to promote disease susceptibility. Xoo strain PXO99A encodes nineteen TALEs, but the host targets of all these TALEs have not been defined. A meta-analysis of rice transcriptome profiles revealed a gene annotated as flavonol synthase/flavanone-3 hydroxylase (henceforth OsS5H/FNS-03g) to be highly induced upon Xoo infection. Further analyses revealed that this gene is induced by PXO99A using Tal9b, a broadly conserved TALE of Xoo. Disruption of tal9b rendered PXO99A less virulent. OsS5H/FNS-03g functionally complemented its Arabidopsis homologue AtDMR6, a well-studied disease susceptibility locus. Biochemical analyses suggested that OsS5H/FNS-03g is a bifunctional protein with Salicylic Acid 5' Hydroxylase (S5H) and Flavone Synthase-I (FNS-I) activities. Further, an exogenous application of apigenin on rice leaves, the flavone that is enzymatically produced by OsS5H/FNS-03g, promoted virulence of PXO99A tal9b-. Overall, our study suggests that OsS5H/FNS-03g is a bifunctional enzyme and its product apigenin is potentially involved in promoting Xoo virulence.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Effects of Local dsRNA Application on Systemic Beet Mosaic Virus (BtMV) Resistance in Nicotiana benthamiana and Beta vulgaris. 局部应用dsRNA对烟叶和甜菜系统性抗BtMV的差异效应

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-07 DOI: 10.1094/MPMI-01-25-0009-R

Dennis Rahenbrock, Marieke Bode, Mark Varrelmann

{"title":"Differential Effects of Local dsRNA Application on Systemic Beet Mosaic Virus (BtMV) Resistance in Nicotiana benthamiana and Beta vulgaris.","authors":"Dennis Rahenbrock, Marieke Bode, Mark Varrelmann","doi":"10.1094/MPMI-01-25-0009-R","DOIUrl":"https://doi.org/10.1094/MPMI-01-25-0009-R","url":null,"abstract":"Beet mosaic virus (BtMV) is one of several viruses infecting sugar beets and was managed by controlling the vector Myzus persicae with neonicotinoid seed treatment. Following the ban of this measure in 2019 in Europe, alternative control strategies must be researched. One alternative might be the use of RNA interference (RNAi) as a major antiviral defence system. Here we report the selection of target regions using small RNA high throughput sequencing of BtMV infected Beta vulgaris subsp. vulgaris and Nicotiana benthamiana plants, the production of double-stranded RNA (dsRNA), and its effective use in inducing resistance against the mechanically inoculated virus. Both in Escherichia coli HT115 produced dsRNAs for BtMV P1 and nuclear inclusion body b (NIb) induced a high level of resistance, when sprayed before mechanical BtMV inoculation, resulting in an 80% reduction of symptomatic B. vulgaris and N. benthamiana plants. Stem-loop RT-qPCR showed the systemic distribution of dsRNA derived siRNA molecules, but the applied dsRNA remained at the site of application and did not spread within the plant. However, when the virus was inoculated on the next upward leaf to the dsRNA application site, no protective effect was observed. Despite this limitation, the results demonstrate the potential of dsRNA as an effective tool for viral protection in sugar beets, thereby establishing a basis for future developments in systemic delivery and broader field applications.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-Mediated Disruption of CsLIEXP1 Reveals Expansin as a Key Susceptibility Factor for Citrus Canker Disease. CRISPR/ cas9介导的CsLIEXP1断裂揭示扩张蛋白是柑橘溃疡病的关键易感因子

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-04-02 DOI: 10.1094/MPMI-12-24-0151-R

Reinaldo Rodrigues de Souza-Neto, Lidia Nascimento Cavalcante, Isis Gabriela Barbosa Carvalho, Maiara Curtolo, Celso Eduardo Benedetti, Marco Aurelio Takita, Nian Wang, Alessandra Alves de Souza

{"title":"CRISPR/Cas9-Mediated Disruption of CsLIEXP1 Reveals Expansin as a Key Susceptibility Factor for Citrus Canker Disease.","authors":"Reinaldo Rodrigues de Souza-Neto, Lidia Nascimento Cavalcante, Isis Gabriela Barbosa Carvalho, Maiara Curtolo, Celso Eduardo Benedetti, Marco Aurelio Takita, Nian Wang, Alessandra Alves de Souza","doi":"10.1094/MPMI-12-24-0151-R","DOIUrl":"https://doi.org/10.1094/MPMI-12-24-0151-R","url":null,"abstract":"The Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. citri effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance to citrus canker disease. Previous studies revealed that the citrus CsLOB1-INDUCED EXPANSIN 1 gene (CsLIEXP1) is highly and directly upregulated by CsLOB1 in Xanthomonas citri subsp. citri-infected plants. Because expansins are associated with cell wall loosening, potentially facilitating bacterial colonization, the CsLOB1-dependent activation of CsLIEXP1 is thought to contribute to canker symptoms and disease progression. Thus, CsLIEXP1 likely represents a critical canker susceptibility gene. In this study, we employed CRISPR/Cas9 to disrupt the function of CsLIEXP1 by modifying its corresponding coding region in Citrus sinensis cv 'Hamlin' and evaluated the post-infection responses of edited plants. DNA sequencing confirmed edition of CsLIEXP1-edited plant, which exhibited 26.47% of CsLIEXP1 edited sequences. Furthermore, CsLIEXP1 protein accumulation was reduced in CsLIEXP1-edited plants compared to wild-type when infected with X. citri. Leaves of edited plants inoculated with X. citri showed significant less canker symptoms, with lesions limited to the site of bacterial inoculation and less pronounced cellular hypertrophy compared to control plants. Our results show that CsLIEXP1 is a citrus canker S gene that acts downstream of CsLOB1, thus providing new insights into plant-pathogen interactions.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0