Molecular Plant-microbe Interactions最新文献

{"title":"Fortifying Plant Armor: CESA3 Enhances <i>Arabidopsis thaliana</i>'s Defense Against Bacterial Wilt Under Heat Stress.","authors":"Jawahar Singh, Manish Tiwari","doi":"10.1094/MPMI-07-24-0077-CM","DOIUrl":"10.1094/MPMI-07-24-0077-CM","url":null,"abstract":"","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":"37 8","pages":"596-597"},"PeriodicalIF":3.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Precision in Callose Staining: Unveiling the Role of Sirofluor.","authors":"Uwe Conrath","doi":"10.1094/MPMI-04-24-0038-LE","DOIUrl":"10.1094/MPMI-04-24-0038-LE","url":null,"abstract":"<p><p>Callose is a vital component in plant biology, contributing to essential processes like pollen maturation and defense against pathogens. However, misconceptions surrounding callose staining persist, particularly regarding the role of aniline blue. It is now known that commercial aniline blue contains sirofluor, and it is this fluorophore, rather than aniline blue itself, that is responsible for the observed fluorescence during callose detection. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"595"},"PeriodicalIF":3.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Role of Soil Microbes in Herbicide Degradation and Crop Protection.","authors":"Siva Sankari","doi":"10.1094/MPMI-06-24-0067-CM","DOIUrl":"https://doi.org/10.1094/MPMI-06-24-0067-CM","url":null,"abstract":"","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":"37 7","pages":"543-544"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Copy Transposons from a Pathogen Give Rise to a Conserved sRNA Family with a Novel Host Immunity Target.","authors":"Lukas Kunz, Manuel Poretti, Coraline R Praz, Marion C Müller, Michele Wyler, Beat Keller, Thomas Wicker, Salim Bourras","doi":"10.1094/MPMI-10-23-0176-SC","DOIUrl":"10.1094/MPMI-10-23-0176-SC","url":null,"abstract":"<p><p>Small RNAs (sRNAs) are involved in gene silencing in multiple ways, including through cross-kingdom transfers from parasites to their hosts. Little is known about the evolutionary mechanisms enabling eukaryotic microbes to evolve functional mimics of host small regulatory RNAs. Here, we describe the identification and functional characterization of <i>SINE_sRNA1</i>, an sRNA family derived from highly abundant short interspersed nuclear element (SINE) retrotransposons in the genome of the wheat powdery mildew pathogen. <i>SINE_sRNA1</i> is encoded by a sequence motif that is conserved in multiple SINE families and corresponds to a functional plant microRNA (miRNA) mimic targeting <i>Tae_AP1</i>, a wheat gene encoding an aspartic protease only found in monocots. Tae_AP1 has a novel function enhancing both pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), thereby contributing to the cross activation of plant defenses. We conclude that <i>SINE_sRNA1</i> and Tae_AP1 are functional innovations, suggesting the contribution of transposons to the evolutionary arms race between a parasite and its host. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"545-551"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small RNA and Degradome Deep Sequencing Reveal Regulatory Roles of MicroRNAs in Response to Sugarcane Mosaic Virus Infection on Two Contrasting Sugarcane Cultivars.","authors":"Yuan Yuan, Cuilin Huang, Kaiyuan Pan, Wei Yao, Rui Xia, Muqing Zhang","doi":"10.1094/MPMI-12-23-0220-R","DOIUrl":"10.1094/MPMI-12-23-0220-R","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) play an essential regulatory role in plant-virus interaction. However, few studies have focused on the roles of miRNAs and their targets after sugarcane mosaic virus (SCMV) infection in sugarcane. To address this issue, we conducted small RNA (sRNA) and degradome sequencing on two contrasting sugarcanes (SCMV-resistant 'Fuoguo1' [FG1] and susceptible 'Badila') infected by SCMV at five time points. A total of 1,578 miRNAs were profiled from 30 sRNA libraries, comprising 660 known miRNAs and 380 novel miRNAs. Differential expression analysis of miRNAs revealed that most were highly expressed during the SCMV exponential phase in Badila at 18 h postinfection, with expression profiles positively correlated with virus replication dynamics as observed through clustering. Analysis of degradome data indicated a higher number of differential miRNA targets in Badila compared to FG1 at 18 h postinfection. Gene ontology (GO) enrichment analysis significantly enriched the stimulus-response pathway, suggesting negative regulatory roles to SCMV resistance. Specifically, miR160 upregulated expression patterns and validated in Badila through quantitative real-time PCR (qRT-PCR) in the early stages of SCMV multiplication. Our research provides new insights into the dynamic response of plant miRNA and virus replication and contributes valuable information on the intricate interplay between miRNAs and SCMV infection dynamics. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"583-593"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize Root Exudates Promote <i>Bacillus</i> sp. Za Detoxification of Diphenyl Ether Herbicides by Enhancing Colonization and Biofilm Formation.","authors":"Yanning Tian, Fangya Zhong, Na Shang, Houyu Yu, Dongmei Mao, Xing Huang","doi":"10.1094/MPMI-02-24-0020-R","DOIUrl":"10.1094/MPMI-02-24-0020-R","url":null,"abstract":"<p><p>Diphenyl ether herbicides are extensively utilized in agricultural systems, but their residues threaten the health of sensitive rotation crops. Functional microbial strains can degrade diphenyl ether herbicides in the rhizosphere of crops, facilitating the restoration of a healthy agricultural environment. However, the interplay between microorganisms and plants in diphenyl ether herbicides degradation remains unclear. Thus, the herbicide-degrading strain <i>Bacillus</i> sp. Za and the sensitive crop, maize, were employed to uncover the interaction mechanism. The degradation of diphenyl ether herbicides by strain <i>Bacillus</i> sp. Za was promoted by root exudates. The strain induced root exudate re-secretion in diphenyl ether herbicide-polluted maize. We further showed that root exudates enhanced the rhizosphere colonization and the biofilm biomass of strain Za, augmenting its capacity to degrade diphenyl ether herbicide. Root exudates regulated gene <i>fliZ</i>, which is pivotal in biofilm formation. Wild-type strain Za significantly reduced herbicide toxicity to maize compared to the ZaΔ<i>fliZ</i> mutant. Moreover, root exudates promoted strain Za growth and chemotaxis, which was related to biofilm formation. This mutualistic relationship between the microorganisms and the plants demonstrates the significance of plant-microbe interactions in shaping diphenyl ether herbicide degradation in rhizosphere soils. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 \"No Rights Reserved\" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"552-560"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oomycete Metabolism Is Highly Dynamic and Reflects Lifestyle Adaptations.","authors":"Sander Y A Rodenburg, Dick de Ridder, Francine Govers, Michael F Seidl","doi":"10.1094/MPMI-12-23-0200-R","DOIUrl":"10.1094/MPMI-12-23-0200-R","url":null,"abstract":"<p><p>The selective pressure of pathogen-host symbiosis drives adaptations. How these interactions shape the metabolism of pathogens is largely unknown. Here, we use comparative genomics to systematically analyze the metabolic networks of oomycetes, a diverse group of eukaryotes that includes saprotrophs as well as animal and plant pathogens, with the latter causing devastating diseases with significant economic and/or ecological impacts. In our analyses of 44 oomycete species, we uncover considerable variation in metabolism that can be linked to lifestyle differences. Comparisons of metabolic gene content reveal that plant pathogenic oomycetes have a bipartite metabolism consisting of a conserved core and an accessory set. The accessory set can be associated with the degradation of defense compounds produced by plants when challenged by pathogens. Obligate biotrophic oomycetes have smaller metabolic networks, and taxonomically distantly related biotrophic lineages display convergent evolution by repeated gene losses in both the conserved as well as the accessory set of metabolisms. When investigating to what extent the metabolic networks in obligate biotrophs differ from those in hemibiotrophic plant pathogens, we observe that the losses of metabolic enzymes in obligate biotrophs are not random and that gene losses predominantly influence the terminal branches of the metabolic networks. Our analyses represent the first metabolism-focused comparison of oomycetes at this scale and will contribute to a better understanding of the evolution of oomycete metabolism in relation to lifestyle adaptation. Numerous oomycete species are devastating plant pathogens that cause major damage in crops and natural ecosystems. Their interactions with hosts are shaped by strong selection, but how selection affects adaptation of the primary metabolism to a pathogenic lifestyle is not yet well established. By pan-genome and metabolic network analyses of distantly related oomycete pathogens and their nonpathogenic relatives, we reveal considerable lifestyle- and lineage-specific adaptations. This study contributes to a better understanding of metabolic adaptations in pathogenic oomycetes in relation to lifestyle, host, and environment, and the findings will help in pinpointing potential targets for disease control. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"571-582"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Soybean <i>Rpp3</i> Gene Encodes a TIR-NBS-LRR Protein that Confers Resistance to <i>Phakopsora pachyrhizi</i>.","authors":"Mandy D Bish, Sowmya R Ramachandran, Amy Wright, Lori M Lincoln, Steven A Whitham, Michelle A Graham, Kerry F Pedley","doi":"10.1094/MPMI-01-24-0007-R","DOIUrl":"10.1094/MPMI-01-24-0007-R","url":null,"abstract":"<p><p>Soybean rust is an economically significant disease caused by the fungus <i>Phakopsora pachyrhizi</i> that negatively impacts soybean (<i>Glycine max</i> [L.] Merr.) production throughout the world. Susceptible plants infected by <i>P. pachyrhizi</i> develop tan-colored lesions on the leaf surface that give rise to funnel-shaped uredinia as the disease progresses. While most soybean germplasm is susceptible, seven genetic loci (<i>Rpp1</i> to <i>Rpp7</i>) that provide race-specific resistance to <i>P. pachyrhizi</i> (<i>Rpp</i>) have been identified. <i>Rpp3</i> was first discovered and characterized in the soybean accession PI 462312 (Ankur), and it was also determined to be one of two <i>Rpp</i> genes present in PI 506764 (Hyuuga). Genetic crosses with PI 506764 were later used to fine-map the <i>Rpp3</i> locus to a 371-kb region on chromosome 6. The corresponding region in the susceptible Williams 82 (Wm82) reference genome contains several homologous nucleotide binding site-leucine rich repeat (NBS-LRR) genes. To identify <i>Rpp3</i>, we designed oligonucleotide primers to amplify <i>Rpp3 candidate</i> (<i>Rpp3C</i>) NBS-LRR genes at this locus from PI 462312, PI 506764, and Wm82 using polymerase chain reaction (PCR). Five <i>Rpp3C</i> genes were identified in both <i>Rpp3</i>-resistant soybean lines, and co-silencing these genes compromised resistance to <i>P. pachyrhizi</i>. Gene expression analysis and sequence comparisons of the <i>Rpp3C</i> genes in PI 462312 and PI 506764 suggest that a single candidate gene, <i>Rpp3C3</i>, is responsible for <i>Rpp3</i>-mediated resistance. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 \"No Rights Reserved\" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"561-570"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thiosulfinate Tolerance Gene Clusters Are Common Features of <i>Burkholderia</i> Onion Pathogens.","authors":"Sujan Paudel, Mei Zhao, Shaun P Stice, Bhabesh Dutta, Brian H Kvitko","doi":"10.1094/MPMI-01-24-0005-R","DOIUrl":"10.1094/MPMI-01-24-0005-R","url":null,"abstract":"<p><p><i>Burkholderia gladioli</i> pv. <i>alliicola</i>, <i>B. cepacia</i>, and <i>B. orbicola</i> are common bacterial pathogens of onion. Onions produce organosulfur thiosulfinate defensive compounds after cellular decompartmentalization. Using whole-genome sequencing and in silico analysis, we identified putative thiosulfinate tolerance gene (TTG) clusters in multiple onion-associated <i>Burkholderia</i> species similar to those characterized in other <i>Allium</i>-associated bacterial endophytes and pathogens. Sequence analysis revealed the presence of three <i>Burkholderia</i> TTG cluster types, with both Type A and Type B being broadly distributed in <i>B. gladioli</i>, <i>B. cepacia</i>, and <i>B. orbicola</i> in both the chromosome and plasmids. Based on isolate natural variation and generation of isogenic strains, we determined the in vitro and in vivo contribution of TTG clusters in <i>B. gladioli</i>, <i>B. cepacia</i>, and <i>B. orbicola</i>. The <i>Burkholderia</i> TTG clusters contributed to enhanced allicin tolerance and improved growth in filtered onion extracts by all three species. TTG clusters also made clear contributions to <i>B. gladioli</i> foliar necrosis symptoms and bacterial populations. Surprisingly, the TTG cluster did not contribute to bacterial populations in onion bulb scales by these three species. Based on our findings, we hypothesize onion-associated <i>Burkholderia</i> may evade or inhibit the production of thiosulfinates in onion bulb tissues. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"507-519"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140137040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Secreted in Xylem 6</i> (<i>SIX6</i>) Mediates <i>Fusarium oxysporum</i> f. sp. <i>fragariae</i> Race 1 Avirulence on <i>FW1</i>-Resistant Strawberry Cultivars.","authors":"Christine Jade Dilla-Ermita, Polly Goldman, Amy Anchieta, Mitchell J Feldmann, Dominique D A Pincot, Randi A Famula, Mishi Vachev, Glenn S Cole, Steven J Knapp, Steven J Klosterman, Peter M Henry","doi":"10.1094/MPMI-02-24-0012-R","DOIUrl":"10.1094/MPMI-02-24-0012-R","url":null,"abstract":"<p><p><i>Fusarium oxysporum</i> f. sp. <i>fragariae</i> (<i>Fof</i>) race 1 is avirulent on cultivars with the dominant resistance gene <i>FW1</i>, while <i>Fof</i> race 2 is virulent on <i>FW1</i>-resistant cultivars. We hypothesized there was a gene-for-gene interaction between a gene at the <i>FW1</i> locus and an avirulence gene (<i>AvrFW1</i>) in <i>Fof</i> race 1. To identify a candidate <i>AvrFW1</i>, we compared genomes of 24 <i>Fof</i> race 1 and three <i>Fof</i> race 2 isolates. We found one candidate gene that was present in race 1, was absent in race 2, was highly expressed in planta, and was homologous to a known effector, <i>secreted in xylem 6</i> (<i>SIX6</i>). We knocked out <i>SIX6</i> in two <i>Fof</i> race 1 isolates by homologous recombination. All <i>SIX6</i> knockout transformants (Δ<i>SIX6</i>) gained virulence on <i>FW1/fw1</i> cultivars, whereas ectopic transformants and the wildtype isolates remained avirulent. Δ<i>SIX6</i> isolates were quantitatively less virulent on <i>FW1/fw1</i> cultivars Fronteras and San Andreas than <i>fw1/fw1</i> cultivars. Seedlings from an <i>FW1/fw1</i> × <i>fw1/fw1</i> population were genotyped for <i>FW1</i> and tested for susceptibility to a <i>SIX6</i> knockout isolate. Results suggested that additional minor-effect quantitative resistance genes could be present at the <i>FW1</i> locus. This work demonstrates that <i>SIX6</i> acts as an avirulence factor interacting with a resistance gene at the <i>FW1</i> locus. The identification of <i>AvrFW1</i> enables surveillance for <i>Fof</i> race 2 and provides insight into the mechanisms of <i>FW1-</i>mediated resistance. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"530-541"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}