Molecular Plant-microbe Interactions最新文献

An RGAE Homolog in Fusarium graminearum Is Critical for Initial Infection in Wheat and Barley. 小麦和大麦镰刀菌RGAE同源物是小麦和大麦初侵染的关键。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-10-06 DOI: 10.1094/MPMI-03-25-0027-R

Nicholas Rhoades, Todd A Naumann, Hye-Seon Kim, Gabdiel Yulfo-Soto, Susan McCormick, Michael J Bowman, Martha Vaughn, Guixia Hao

{"title":"An RGAE Homolog in Fusarium graminearum Is Critical for Initial Infection in Wheat and Barley.","authors":"Nicholas Rhoades, Todd A Naumann, Hye-Seon Kim, Gabdiel Yulfo-Soto, Susan McCormick, Michael J Bowman, Martha Vaughn, Guixia Hao","doi":"10.1094/MPMI-03-25-0027-R","DOIUrl":"10.1094/MPMI-03-25-0027-R","url":null,"abstract":"Fusarium graminearum is the primary causal agent of Fusarium head blight (FHB), a devastating fungal disease on wheat, barley, and other grains. During infection, F. graminearum produces trichothecene mycotoxins, predominately deoxynivalenol (DON), that contaminate grain and reduce grain yield and quality. Although DON functions as a virulence factor to promote F. graminearum spread in the wheat head, it is not essential for establishing initial infection in wheat or barley. When fungal pathogens, such as F. graminearum, infect a host plant, they secrete hundreds of protein effectors that interfere with plant immunity to promote disease. A recent study identified hundreds of putative effector-encoding genes that are conserved across six Fusarium species. In the current study, we selected a subset of 50 conserved effectors from F. graminearum PH-1 and determined their expression on wheat heads over a 7-day infection period. Gene expression analysis revealed that several genes were highly induced in wheat heads during fungal infection. One of them was a putative rhamnogalacturonan acetylesterase homolog (FgRGAE), which was also highly induced in barley heads. FHB virulence assays showed that deletion mutants of FgRGAE significantly reduced initial infection and DON accumulation in wheat and barley heads compared with wild-type controls. Replacing the FgRGAE::Hyg deletion construct with an FgRGAEORF+::Gen construct at the native locus restored FHB disease to wild-type levels in both wheat and barley heads. FgRGAE may serve as an ideal target to reduce FHB and mycotoxin contamination in wheat and barley. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"MPMI03250027R"},"PeriodicalIF":3.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conservation of Genes Required for Arbuscular Mycorrhizal Symbiosis. 丛枝菌根共生所需基因的保存。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-10-01 DOI: 10.1094/MPMI-05-25-0065-CR

Ellen Krall, Katherine Benza, Raymond Kannenberg, Karina Medina-Jimenez, Srijana Mukhia, Vincent Vanyo, Armando Bravo

{"title":"Conservation of Genes Required for Arbuscular Mycorrhizal Symbiosis.","authors":"Ellen Krall, Katherine Benza, Raymond Kannenberg, Karina Medina-Jimenez, Srijana Mukhia, Vincent Vanyo, Armando Bravo","doi":"10.1094/MPMI-05-25-0065-CR","DOIUrl":"https://doi.org/10.1094/MPMI-05-25-0065-CR","url":null,"abstract":"Arbuscular mycorrhizal (AM) symbiosis is an ancient association that played a key role in the adaptation of plants to terrestrial environments. Originating over 400 million years ago at the dawn of land plants, this interaction depends on a core set of conserved genes that enable hosts to establish and maintain symbiotic relationships with AM fungi. The AM symbiotic program includes distinct genetic components for each stage of development, from signal perception to nutrient exchange. While AM-host plants have retained key genes dedicated to symbiosis, non-host lineages have independently lost these genes multiple times over evolutionary history. Recent studies in the liverwort Marchantia paleacea demonstrate that core mechanisms underlying AM symbiosis are conserved from bryophytes to angiosperms. Comparative genomic studies continue to uncover how symbiosis-specific genes are integrated with broadly conserved cellular machinery to sustain this interaction. Understanding these deeply conserved genetic modules is essential for uncovering the evolutionary foundations of plant-microbe associations and for harnessing their potential in sustainable agriculture.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Single-Nucleus Gene Expression Atlas of Grapevine and Erysiphe necator During Early Powdery Mildew Infection. 葡萄和丹参在早期白粉病感染过程中的双单核基因表达图谱。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-10-01 DOI: 10.1094/MPMI-08-25-0099-R

Maria-Sole Bonarota, Jadran F Garcia, Mélanie Massonnet, Mirella Zaccheo, Rosa Figueroa-Balderas, Noé Cochetel, Dario Cantu

{"title":"Dual Single-Nucleus Gene Expression Atlas of Grapevine and Erysiphe necator During Early Powdery Mildew Infection.","authors":"Maria-Sole Bonarota, Jadran F Garcia, Mélanie Massonnet, Mirella Zaccheo, Rosa Figueroa-Balderas, Noé Cochetel, Dario Cantu","doi":"10.1094/MPMI-08-25-0099-R","DOIUrl":"https://doi.org/10.1094/MPMI-08-25-0099-R","url":null,"abstract":"We applied single-nucleus transcriptomics to study how Erysiphe necator (causal agent of powdery mildew) infects grapevine leaves at one- and five- days post infection, including controls and three biological replicates. We generated a grapevine leaf atlas encompassing over 100,000 nuclei, and a pathogen atlas of more than 3,000 nuclei. We successfully annotated all major grapevine cell types, including mesophyll, epidermis, phloem and xylem parenchyma, companion cells, and guard cells. We identified key E. necator structures, including appressoria, haustoria, and hyphae, and provided a list of novel cell type markers for both species. We reveal structure-specific gene expression programs in E. necator laying a foundation for future studies of fungal development and virulence mechanisms. In the host, we identified spatially distinct expression patterns of defense-related genes. As the infection progressed, we observed the activation of a coordinated immune response involving multiple cell types, mainly epidermal and mesophyll cells. High-dimensional weighted gene co-expression network analysis identified key hubs and networks associated with cell type-specific signaling and defense response. We describe a spatial separation of pattern- and effector- triggered immunity, supporting a model in which pattern-triggered immunity is activated at the site of pathogen contact and effector-triggered immunity is induced in surrounding tissue.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptomic Analysis of Zymoseptoria tritici Reveals Interaction-Specific Gene Expression Patterns During Susceptible, Resistant, and Non-Host Interactions. 小麦酵母菌的比较转录组学分析揭示了在易感、抗性和非宿主相互作用中相互作用特异性基因表达模式。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-26 DOI: 10.1094/MPMI-07-25-0090-R

Sandra V Gomez-Gutierrez, Cassidy R Million, Namrata Jaiswal, Michael Gribskov, Matthew Helm, Stephen B Goodwin

{"title":"Comparative Transcriptomic Analysis of Zymoseptoria tritici Reveals Interaction-Specific Gene Expression Patterns During Susceptible, Resistant, and Non-Host Interactions.","authors":"Sandra V Gomez-Gutierrez, Cassidy R Million, Namrata Jaiswal, Michael Gribskov, Matthew Helm, Stephen B Goodwin","doi":"10.1094/MPMI-07-25-0090-R","DOIUrl":"https://doi.org/10.1094/MPMI-07-25-0090-R","url":null,"abstract":"Zymoseptoria tritici causes Septoria tritici blotch which significantly reduces yields of wheat. To investigate infection phase-specific gene expression in the pathogen, we analyzed gene expression during infection of susceptible and resistant wheat cultivars, plus the non-host species barley at 1, 3, 6, 10, 17 and 23 days post inoculation (DPI). There were dramatic dif-ferences in pathogen gene expression at 10 DPI in the susceptible compared to both re-sistant interactions. The most pronounced differences in pathogen gene expression were observed at 3 DPI in both the susceptible and resistant host interactions compared to the non-host. Thirty-one putative effectors showed early expression during the susceptible compared to the non-host interaction; six were selected for subcellular localization studies. Using Agrobacterium-mediated transient expression in Nicotiana benthamiana, subcellular localization assays revealed that two candidate effectors localized to putative mobile cyto-solic bodies when expressed without their signal peptides, suggesting potential roles in in-tracellular signaling or host gene regulation. When expressed with their signal peptides, four candidate effectors localized to the cytosol, while one did not accumulate to detectable levels. Comparison of pathogen gene expression in the susceptible host to expression in the resistant hosts identified genes expressed during the transition from biotrophic to necrot-rophic growth at 10 DPI. Comparison of pathogen gene expression in resistant and suscep-tible hosts, versus in the non-host barley, identified genes involved in initial colonization and host recognition. These results contribute to understanding candidate effectors that are activated early during infection and may play a role in the suppression of plant immunity.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MoHTR1, a Nuclear Effector of the Rice Blast Fungus, Regulates Alternative Splicing of Rice Immunity Genes. 稻瘟病菌核效应子MoHTR1调控水稻免疫基因的选择性剪接。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-23 DOI: 10.1094/MPMI-03-25-0033-SC

You-Jin Lim, Joo Hyun Lee, Jaeho Ko, Doil Choi, Yong-Hwan Lee

{"title":"MoHTR1, a Nuclear Effector of the Rice Blast Fungus, Regulates Alternative Splicing of Rice Immunity Genes.","authors":"You-Jin Lim, Joo Hyun Lee, Jaeho Ko, Doil Choi, Yong-Hwan Lee","doi":"10.1094/MPMI-03-25-0033-SC","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0033-SC","url":null,"abstract":"Plant pathogens deploy nuclear effectors to manipulate the host immune response and reprogram cellular processes. Alternative splicing, a key RNA processing mechanism, plays a pivotal role in determining the fate of immune response-associated genes. Some nuclear effectors have been studied for their roles in the transcription or post-transcriptional regulation of host genes. However, the understanding of how a single nuclear effector engages in multiple regulatory processes within the host nucleus remains limited. Previously, we demonstrated that MoHTR1, a nuclear effector of the rice blast fungus, binds to effector binding elements in the promoters of target genes and modulates host immunity. To further explore the multifunctionality of MoHTR1, we identified host proteins interacting with MoHTR1. MoHTR1 interacts with the splicing factor OsSR45 in rice nuclear speckles and promotes degradation of OsSR45. MoHTR1 is involved in alternative splicing of mRNAs associated with immune and stress responses. The changes in alternative splicing patterns of these mRNAs are identical in both Magnaporthe oryzae-infected rice and MoHTR1-overexpressing transgenic lines, underscoring the consistent regulatory effect of MoHTR1. Our findings highlight dual roles of MoHTR1 in regulating both transcription and post-transcriptional processes, and providing novel insights into how nuclear effectors modulate host immunity through intricate molecular mechanisms.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Activation of RNAi Reveals Genomic Regions of Grapevine Red Blotch Virus Targeted for Silencing in Grapevine. 早期激活的RNAi揭示了葡萄红斑病病毒沉默的目标基因组区域。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-23 DOI: 10.1094/MPMI-04-25-0038-R

Christian Mandelli, Laurent G Deluc

{"title":"Early Activation of RNAi Reveals Genomic Regions of Grapevine Red Blotch Virus Targeted for Silencing in Grapevine.","authors":"Christian Mandelli, Laurent G Deluc","doi":"10.1094/MPMI-04-25-0038-R","DOIUrl":"10.1094/MPMI-04-25-0038-R","url":null,"abstract":"Grapevine red blotch virus (GRBV), a member of the Geminiviridae family that causes reduced fruit quality and yield, is an emerging challenge for the wine industry. Viticultural practices and pest management have been largely ineffective at mitigating the impacts of GRBV, necessitating alternative control strategies. Here, we investigated the early activation of RNA interference (RNAi) in GRBV-infected grapevines and, through small RNA sequencing, identified nine genomic virus-derived small-interfering RNA (vsiRNA)-producing regions referred to as hotspots (HSs). Subsequent analyses revealed that these HSs were primarily involved in producing 24-nt vsiRNA species associated with transcriptional gene silencing toward later stages of infection. Double-stranded RNA molecules derived from these HSs were administered to GRBV-infected plants via root soaking, significantly (P < 0.05) reducing viral gene expression in leaves and petioles for up to 1 month. Ultimately, we assessed the potential of viral mutation within these HSs, identified areas of higher mutational entropy, and found that most HS locations are within viral regions with lower probabilities of mutation events. These findings provide the basis for future research to characterize the role of small RNA-induced silencing mechanisms in grapevine-GRBV interactions and their potential translation for field-based technology, such as RNAi biopesticides, to manage red blotch disease. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"MPMI04250038R"},"PeriodicalIF":3.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated Haustoria Segmentation Enables Rapid Gene Function Analysis in Cereal-Powdery Mildew Pathosystems. 加速吸器分割使谷物-白粉病病理系统的快速基因功能分析成为可能。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-22 DOI: 10.1094/MPMI-06-25-0067-TA

Stefanie Lück, Deniz Demirhan, Laura Agsten, Ahmed Raza Khan, Oksana Maier, Dimitar K Douchkov

{"title":"Accelerated Haustoria Segmentation Enables Rapid Gene Function Analysis in Cereal-Powdery Mildew Pathosystems.","authors":"Stefanie Lück, Deniz Demirhan, Laura Agsten, Ahmed Raza Khan, Oksana Maier, Dimitar K Douchkov","doi":"10.1094/MPMI-06-25-0067-TA","DOIUrl":"https://doi.org/10.1094/MPMI-06-25-0067-TA","url":null,"abstract":"Reliable, high-throughput quantification of early fungal infection events is crucial for gene-function studies, but it remains labor-intensive. We report an open-source pipeline that automates the detection of β-glucuronidase (GUS-stained) epidermal cells and the intracellular haustoria formed by powdery mildew on barley and wheat leaves. Whole-slide images are captured with a commercial scanner, focus-projected, tiled, and analyzed by deep-learning models trained on expertly annotated datasets. A You Only Look Once (YOLO) network identifies GUS-positive cells, while a companion segmentation model pinpoints haustoria within each cell; automatic focus-layer selection preserves fine structural detail. The workflow runs in minutes per slide on a single workstation and maintains near-perfect agreement with manual counts in both barley and wheat, demonstrating robust cross-species transferability. By delivering single-cell readouts with minimal user input, the pipeline enables rapid functional validation screens and supports large-scale phenotyping of cereal-powdery mildew interactions.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Novel Genetic Resources for Broad-Based Soybean Cyst Nematode Resistance Independent of Conventional Loci. 与传统基因座无关的大豆包囊线虫抗性新遗传资源的鉴定。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-22 DOI: 10.1094/MPMI-06-25-0069-FI

Sushil Satish Chhapekar, Vikas Devkar, Amir W Khan, Heng Ye, Sonam Singh, Naoufal Lakhssassi, Tri D Vuong, Khalid Meksem, Henry T Nguyen, Gunvant B Patil

{"title":"Identification of Novel Genetic Resources for Broad-Based Soybean Cyst Nematode Resistance Independent of Conventional Loci.","authors":"Sushil Satish Chhapekar, Vikas Devkar, Amir W Khan, Heng Ye, Sonam Singh, Naoufal Lakhssassi, Tri D Vuong, Khalid Meksem, Henry T Nguyen, Gunvant B Patil","doi":"10.1094/MPMI-06-25-0069-FI","DOIUrl":"https://doi.org/10.1094/MPMI-06-25-0069-FI","url":null,"abstract":"Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most economically damaging soil-borne pathogen affecting soybean, causing significant yield losses in the United States and worldwide. Current commercial cultivars rely heavily on a limited genetic resistance base, primarily from PI 88788 and Peking, which has led to the emergence of virulent SCN populations that threaten the durability of this resistance. To address this challenge, we performed a comprehensive allelic analysis of key resistance loci (rhg1, Rhg4, qSCN10 (O), and qSCN18 (G)) using whole-genome resequencing data from 1,110 diverse soybean accessions. Our study identified novel non-synonymous SNPs in 27 accessions, including PI 602492 (Glycine max) and two Glycine soja accessions (PI 522226, PI 522228), which display strong to moderate resistance across multiple SCN HG types (Heterodera glycines) or SCN races. Additionally, we identified two G. soja accessions, PI 507380B and PI 507752 that exhibited strong resistance to HG type 2.5.7 (Race 5). Notably, accessions with genotypes similar to these five showed variable resistance phenotypes, suggesting the presence of additional, yet unidentified, genes contributing to broad-based SCN resistance. Among these, PI 602492 stands out as a valuable new resistance source with strong activity against multiple HG types (races), making it an excellent candidate for gene discovery and breeding efforts to enhance resistance independently of conventional germplasm. These findings provide important, underutilized genetic resources that can expand the resistance base and drive the development of more durable SCN-resistant soybean cultivars.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multilevel Analysis of Response to Plant Growth Promoting and Pathogenic Bacteria in Arabidopsis Roots. 拟南芥根系对植物促生和致病菌反应的多水平分析。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-22 DOI: 10.1094/MPMI-09-25-0125-R

Anna Koprivova, Miroslav Berka, Veronika Berková, Daniela Ristova, Gözde Merve Türksoy, Melina Schwier, Philipp Westhoff, Martin Cerny, Stanislav Kopriva

{"title":"Multilevel Analysis of Response to Plant Growth Promoting and Pathogenic Bacteria in Arabidopsis Roots.","authors":"Anna Koprivova, Miroslav Berka, Veronika Berková, Daniela Ristova, Gözde Merve Türksoy, Melina Schwier, Philipp Westhoff, Martin Cerny, Stanislav Kopriva","doi":"10.1094/MPMI-09-25-0125-R","DOIUrl":"https://doi.org/10.1094/MPMI-09-25-0125-R","url":null,"abstract":"A major challenge in plant-microbe interaction research is understanding how plants distinguish between commensal and pathogenic microorganisms. We compared Arabidopsis responses to two contrasting bacterial strains, the plant growth promoting (PGP) Pseudomonas sp. CH267 and the pathogen Burkholderia glumeae PG1, using integrated multi-omics analyses. The pathogen triggered stronger transcriptional reprogramming and proteomic changes in roots than the PGP strain, while both strains also affected numerous leaf proteins indicating systemic responses. Interaction with both strains increased the abundance of sulfur containing metabolites: camalexin, glutathione, and cysteine, in particular under pathogen treatment, which corresponded with elevated total sulfur in the leaves. Root and root exudate metabolomes significantly changed, with amino acids and tricarboxylic acid cycle intermediates accumulating in roots but being diminished in the exudates. Integrative analysis across the omics datasets revealed strong correlations between metabolite levels, protein abundance, and transcript levels, highlighting new links between sulfur metabolism, defense pathways, and mineral nutrition, including iron. Together, these findings uncover the complex multi-layered nature of Arabidopsis responses to commensal and pathogenic bacteria and identify new connections across different regulatory levels.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RBL1 Shapes Phyllosphere Microbial Structure to Enhance Disease Resistance in Rice. RBL1改变水稻层球微生物结构增强抗病性

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-09-20 DOI: 10.1094/MPMI-08-25-0097-R

Meng Liu, Xinyu Han, Anum Bashir, Fengdie Xia, Qiping Sun, Guang Chen, Peng Sun, Tom Hsiang, Xiaowei Han, Qiang Li, Kanbin Xie, Guotian Li

{"title":"RBL1 Shapes Phyllosphere Microbial Structure to Enhance Disease Resistance in Rice.","authors":"Meng Liu, Xinyu Han, Anum Bashir, Fengdie Xia, Qiping Sun, Guang Chen, Peng Sun, Tom Hsiang, Xiaowei Han, Qiang Li, Kanbin Xie, Guotian Li","doi":"10.1094/MPMI-08-25-0097-R","DOIUrl":"https://doi.org/10.1094/MPMI-08-25-0097-R","url":null,"abstract":"Rice blast is caused by the fungus Magnaporthe oryzae and seriously threatens rice production worldwide. Harnessing microbe-mediated resistance is a key strategy in disease control. The RBL1 gene of rice encodes a cytidine diphosphate diacylglycerol synthase, and editing of RBL1 resulted in a new allele named RBL1Δ12, which confers multipathogen resistance and maintains yield. This study demonstrated that enhanced blast resistance of rbl1Δ12 partially stemmed from differences in phyllosphere microbiota. The rbl1Δ12 line exhibited enrichment of beneficial microorganisms in the phyllosphere, such as those from the genera Pantoea, Pseudomonas, Acidobacteria, and Sphingomonas, which inhibited the growth of several rice pathogens in dual culture plates. Moreover, phyllosphere microbial interactions were strengthened in rbl1Δ12, contributing toward resistance to M. oryzae. Synthetic microbial communities that mimic rbl1Δ12 microbial communities induced rice immunity and enhanced the resistance to M. oryzae in two rice varieties, achieving an environment-friendly control of rice blast. This study revealed that host genetic modification contributed to shaping microbiome composition, providing a strategy based on beneficial microbes for sustainable disease control.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0