Zymoseptoria tritici Shows Local Differences in Within-Field Diversity and Effector Variation.

Abstract

Zymoseptoria tritici is a cosmopolitan hemibiotrophic wheat pathogen with a high mutation rate and a mixed reproduction system, with sexual and asexual cycles occurring within the same disease cycle. This leads to challenges in traditional farming management. For successful integrated pest management, especially for surveys of new aggressive lineages, it is critical to understand population diversity in the field. We look at whole-genome sequence data for three datasets to differentiate within field diversity in fields of similar size: one dataset from a newly sampled field population from the United Kingdom and two publicly available datasets from fields from the United States and Switzerland. This survey of genetic variation allows us to describe in detail how variable the field populations are and offers insight into the dynamics of the disease in a snapshot per field. Inspection of population structure and diversity features, such as minor allele frequency distribution and clonality, show no within-field structure, the most abundant single-nucleotide polymorphisms are present in low frequency, and European fields have higher clonality. Knowing that effectors play particularly important roles in (a)virulence, we specifically assess effector diversity characteristics. On a whole-genome scale, we can see separation of the populations at the regional scale, but we do not find such separation for the effectors. Moreover, we find that multiple effector haplotypes can be found interspersed within the field and even occur within what have been considered clonal isolates or isolates from a single lesion. Our analyses highlight that within-field Z. tritici genetic variation is higher than previously reported. Our finding that multiple effector haplotypes can be found within a single lesion might explain the large resistance gene-breaking potential of Z. tritici. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.