Molecular Plant-microbe Interactions最新文献_第3页

Potato Virus Y Restricts Alternaria solani Growth During Co-Infection. 马铃薯Y病毒在共侵染过程中抑制茄交菌生长。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-30 DOI: 10.1094/MPMI-03-25-0026-R

Pablo A Gutierrez, Joshua Fuller, Sydney Stroschein, Austin VanDenTop, Dennis Halterman, Aurelie M Rakotondrafara

{"title":"Potato Virus Y Restricts Alternaria solani Growth During Co-Infection.","authors":"Pablo A Gutierrez, Joshua Fuller, Sydney Stroschein, Austin VanDenTop, Dennis Halterman, Aurelie M Rakotondrafara","doi":"10.1094/MPMI-03-25-0026-R","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0026-R","url":null,"abstract":"In the environment, multiple microbes can interact with each other in the plant phyllosphere. These associations can shape the plant's development, stress responses, and disease susceptibility, but the molecular mechanisms that govern this process remain unexplained. Of interest are the multiple or successive infections that crop plants are exposed to within a growing season. One of the most common and economically important viruses of potato is potato virus Y (PVY, Potyviridae). We show that PVY infection of potato limited the expansion of foliar necrotic lesions caused by the early blight fungus Alternaria solani. The reduced growth phenotype persisted when the fungal mycelium was transferred to solid growth media. RNAseq analysis of responses in potato and A. solani to the presence of PVY suggested two mechanisms that can explain this interaction. First, in A. solani exposed to PVY-positive leaves, we observed a down-regulation of fungal pathogenicity genes. Second, we found that, in the absence of PVY, A. solani downregulates ethylene-responsive defense in potato, but this effect was eliminated when the host was infected with PVY. Our findings expand our understanding of how pathogen virulence can be affected by other pathogens competing for the same host resources. The observation that PVY can alter A. solani infection illustrates the ecological role of viruses as a potential contributor to the development of disease outbreaks.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three Xanthomonas Cell Wall Degrading Enzymes and Sorghum Brown midrib12 Contribute to Virulence and Resistance in the Bacterial Leaf Streak Pathosystem. 三种黄单胞菌细胞壁降解酶和高粱褐中脉12对细菌条纹病毒力和抗性的影响

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-13 DOI: 10.1094/MPMI-05-24-0051-R

Qi Wang, Kira M Veley, Joshua M B Johnson, Josh Sumner, Gijs van Erven, Mirjam A Kabel, Singha Dhungana, Jeffrey Berry, Adam Boyher, David M Braun, Wilfred Vermerris, Rebecca S Bart

{"title":"Three Xanthomonas Cell Wall Degrading Enzymes and Sorghum Brown midrib12 Contribute to Virulence and Resistance in the Bacterial Leaf Streak Pathosystem.","authors":"Qi Wang, Kira M Veley, Joshua M B Johnson, Josh Sumner, Gijs van Erven, Mirjam A Kabel, Singha Dhungana, Jeffrey Berry, Adam Boyher, David M Braun, Wilfred Vermerris, Rebecca S Bart","doi":"10.1094/MPMI-05-24-0051-R","DOIUrl":"10.1094/MPMI-05-24-0051-R","url":null,"abstract":"With an increasing demand for renewable fuels, bioenergy crops are being developed with high sugar content and altered cell walls to improve processing efficiency. These traits may have unintended consequences for plant disease resistance. Xanthomonas vasicola pv. holcicola (Xvh), the causal agent of sorghum bacterial leaf streak, is a widespread bacterial pathogen. Here, we show that Xvh expresses several bacterial cell wall degrading enzymes (CWDEs) during sorghum infection, and these are required for full virulence. In tolerant sorghum, Xvh infection results in the induction of a key enzyme in monolignol biosynthesis, Brown midrib12 (Bmr12), but this did not affect lignin content nor composition. Mutation of Bmr12 rendered the tolerant genotype susceptible. Bmr12 encodes caffeic acid O-methyltransferase (COMT), an enzyme that generates sinapaldehyde as its major product. Growth inhibition of Xvh in the presence of sinapaldehyde was observed in vitro. We conclude that mutations that alter the components of the sorghum cell wall can reduce sorghum resistance to Xvh and that Xvh CWDEs contribute to bacterial virulence. Given the enhanced bioprocessing characteristics of bmr12 sorghum, these results provide a cautionary tale for current and future efforts aimed at developing dedicated bioenergy crops. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"MPMI05240051R"},"PeriodicalIF":3.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Alternaria alternata Mip1/RAPTOR Mediates Virulence by Regulating Toxin Production and Autophagy. 互交霉Mip1/RAPTOR通过调节毒素产生和自噬介导毒力。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-10 DOI: 10.1094/MPMI-12-24-0161-R

Yu-Ling Huang, Kuang-Ren Chung, Pei-Ching Wu

{"title":"The Alternaria alternata Mip1/RAPTOR Mediates Virulence by Regulating Toxin Production and Autophagy.","authors":"Yu-Ling Huang, Kuang-Ren Chung, Pei-Ching Wu","doi":"10.1094/MPMI-12-24-0161-R","DOIUrl":"https://doi.org/10.1094/MPMI-12-24-0161-R","url":null,"abstract":"The necrotrophic pathogen Alternaria alternata produces a host-selective toxin to attack its host plants. This study characterized the crucial function of the Mip1/RAPTOR ortholog (AaMip1) in toxin production and autophagy formation. AaMip1 physically interacts with the Target of Rapamycin (Tor) protein. In response to nitrogen starvation and H2O2, AaMip1 binds to Tor and triggers autophagy and oxidative stress detoxification. Deleting the AaMip1 gene resulted in a ΔAaMip1 strain that increased sensitivity to various oxidants, decreased the expression of two oxidative-stress-response genes, AaYap1 and AaNoxA, and had lower catalase activity than the wild type. ΔAaMip1 produced lower levels of ACT toxin than the wild type after a 7-day incubation; however, ΔAaMip1 produced tricycloalternarene mycotoxins but not ACT after 21 days. The reduction of ΔAaMip1 virulence in the host plant is due to low ACT production, defective H2O2 detoxification, impaired autophagy, and slow growth during invasion. However, AaMip1 plays a negative role in maintaining cell wall integrity and lipid body accumulation. ΔAaMip1 had thicker cell walls and emitted brighter red fluorescence after staining with the cell-wall disrupting agents Congo red and calcofluor white. ΔAaMip1 was more resistant to these compounds than the wild type under nutrient-rich conditions. The observed defects in the ΔAaMip1 were restored in the complementation (CP) strain after re-expressing a functional copy of AaMip1. This study increases our understanding of how A. alternata deals with toxic ROS, triggers autophagy formation, maintains normal cell wall integrity, and regulates toxin metabolism via the AaMip1-mediated signaling pathways.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of a Transketolase Mutation Leads to Only Partial Restoration of Symbiosis in Sinorhizobium meliloti. 抑制转酮醇酶突变只会部分恢复共生关系。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-10 DOI: 10.1094/MPMI-02-25-0017-R

Sabhjeet Kaur, Justin P Hawkins, Ivan J Oresnik

{"title":"Suppression of a Transketolase Mutation Leads to Only Partial Restoration of Symbiosis in Sinorhizobium meliloti.","authors":"Sabhjeet Kaur, Justin P Hawkins, Ivan J Oresnik","doi":"10.1094/MPMI-02-25-0017-R","DOIUrl":"https://doi.org/10.1094/MPMI-02-25-0017-R","url":null,"abstract":"The interaction between Sinorhizobium meliloti and alfalfa is a well-studied model system for symbiotic establishment between rhizobia and legume plants. Proper utilization of carbon sources has been linked with effective symbiotic establishment in S. meliloti strain Rm1021. Previous work has shown that mutation of the gene tktA, which encodes a transketolase involved in the pentose phosphate pathway, resulted in a strain impaired in many biological functions, including the inability to establish a symbiosis with alfalfa. Work with this strain revealed the appearance of suppressor mutations which could partially revert the symbiotic phenotype associated with a tktA mutation. Characterization of these suppressor strains revealed that carbon phenotypes associated with a mutation in tktA were no longer present and that the production of succinoglycan was partially restored. Central carbon metabolite pools were observed to be different compared to the wildtype and tktA mutant strains. Multiple independent mutations were identified in the gene SMc02340, a Gnt-type negative regulator upon sequencing. RT-PCR suggests that SMc02340 acts as a negative regulator on an operon containing the gene tktB, which becomes upregulated when the suppressor mutation is present or SMc02340 is removed. Microscopic analysis revealed a unique symbiotic phenotype. The tktA mutant strain induced root hair curling but could not colonize the apoplastic space. Collectively the data suggests the upregulation of tktB can partially bypass some blocks associated with a lesion in tktA, including the colonization of the curled root hair, but cannot fully compensate for the loss of tktA.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction Between Barley Yellow Dwarf Virus-GAV Movement Protein and VOZ Proteins Delays Flowering of Plant. 大麦黄矮病毒- gav运动蛋白与VOZ蛋白互作延迟植物开花

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-10 DOI: 10.1094/MPMI-02-25-0013-R

Yilin Zhang, Caiping Huang, Qiang Zeng, Ming Yang, Yanhong Wu, Ye Tao, Shafat Ahmad Ahanger, Hamza Rafiq, Yunfeng Wu, Xingan Hao

引用次数: 0

Cell Wall Dynamics in the Parasitic Plant (Striga) and Rice Pathosystem. 寄生植物（Striga）细胞壁动力学与水稻病理系统。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-01 Epub Date: 2025-04-14 DOI: 10.1094/MPMI-06-24-0064-FI

Damaris Barminga, Sylvia Mutinda, Fredrick M Mobegi, Willy Kibet, Brett Hale, Sylvester Anami, Asela Wijeratne, Emily S Bellis, Steven Runo

{"title":"Cell Wall Dynamics in the Parasitic Plant (Striga) and Rice Pathosystem.","authors":"Damaris Barminga, Sylvia Mutinda, Fredrick M Mobegi, Willy Kibet, Brett Hale, Sylvester Anami, Asela Wijeratne, Emily S Bellis, Steven Runo","doi":"10.1094/MPMI-06-24-0064-FI","DOIUrl":"10.1094/MPMI-06-24-0064-FI","url":null,"abstract":"In the plant-plant pathosystem of rice (Oryza sativa) and the parasitic plant Striga hermonthica, cell walls from either plant are important defensive and offensive structures. Here, we reveal the cell wall dynamics in both Striga and rice using simultaneous RNA sequencing. We used weighted gene co-expression network analysis to home in on cell wall modification processes occurring in interactions with a resistant rice cultivar (Nipponbare) compared with a susceptible one (IAC 165). Likewise, we compared the cell wall dynamics in Striga infecting resistant and susceptible rice. Our study revealed an intense battlement at the Striga-rice cell walls involving both parasite (offense) and host (defense) factors, the outcome of which makes the difference between successful or failed parasitism. Striga activates genes encoding cell wall-degrading enzymes to gain access to the host, expansins to allow for cell elongation, and pectin methyl esterase inhibitors for rigidity during infection. In the susceptible host, immune response processes are not induced, and Striga-derived cell wall-degrading enzymes easily breach the host cell wall, resulting in successful parasitism. In contrast, the resistant host invokes immune responses modulated by phytohormones to fortify the cell wall through polysaccharides and lignin deposition. Through these processes, the cell wall of the resistant host successfully obstructs parasite entry. We discuss the implications of these findings in the context of practical agriculture in which cell wall modification can be used to manage parasitic plants. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"285-296"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine Grain: Molecular, Cellular, and Genomic Details of Cereal Crop Diseases. 精细谷物：谷物作物疾病的分子、细胞和基因组细节。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-01 DOI: 10.1094/MPMI-04-25-0040-CM

Lida Derevnina, Ksenia V Krasileva, Benjamin Schwessinger, Richard A Wilson

引用次数: 0

Molecular Monitoring of Fungicide Resistance in Crop Phytopathogens. 作物病原菌抗杀菌剂的分子监测。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-01 Epub Date: 2025-04-25 DOI: 10.1094/MPMI-09-24-0105-FI

Katherine G Zulak

{"title":"Molecular Monitoring of Fungicide Resistance in Crop Phytopathogens.","authors":"Katherine G Zulak","doi":"10.1094/MPMI-09-24-0105-FI","DOIUrl":"https://doi.org/10.1094/MPMI-09-24-0105-FI","url":null,"abstract":"The fight against crop pathogens relies mainly on host genetics and chemistry; however, both areas are compromised by the evolution of resistance in the pathogen population. Fungicide resistance is an ongoing challenge to global food security, as it threatens these important crop protection chemistries. One critical component of resistance management is an effective detection and monitoring program, which needs to be agile, scalable, sensitive, accurate, and cost effective. A rapidly evolving suite of molecular tools are being developed for the detection of fungicide resistance mutations in phytopathogen populations, including high-throughput PCR-based quantitative assays and cutting-edge third-generation DNA sequencing. A single \"silver bullet\" detection technology that will satisfy all study objectives does not exist; thus, every tool has a niche in an integrated detection and monitoring program. This review presents an overview of the rapidly changing landscape of fungicide resistance detection, illustrates how molecular techniques are being exploited to combat fungicide resistance in cereal crop phytopathogens, and highlights challenges and future research directions to aid in the design of effective monitoring systems that aim to apply fungicides strategically and minimize the cost of resistance. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":"38 2","pages":"160-172"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Lesions to Lessons: Two Decades of Filamentous Plant Pathogen Genomics. 从病变到教训：丝状植物病原体基因组学二十年。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-01 Epub Date: 2025-04-24 DOI: 10.1094/MPMI-09-24-0115-FI

Wagner C Fagundes, Yu-Seng Huang, Sophia Häußler, Thorsten Langner

引用次数: 0

Closing the Information Gap Between the Field and Scientific Literature for Improved Disease Management, with a Focus on Rice and Bacterial Blight. 缩小田间与科学文献之间的信息差距，改善病害管理--重点关注水稻和细菌性枯萎病。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-03-01 Epub Date: 2025-01-24 DOI: 10.1094/MPMI-07-24-0075-FI

Eliza P I Loo, Boris Szurek, Yugander Arra, Melissa Stiebner, Marcel Buchholzer, B N Devanna, Casiana M Vera Cruz, Wolf B Frommer

{"title":"Closing the Information Gap Between the Field and Scientific Literature for Improved Disease Management, with a Focus on Rice and Bacterial Blight.","authors":"Eliza P I Loo, Boris Szurek, Yugander Arra, Melissa Stiebner, Marcel Buchholzer, B N Devanna, Casiana M Vera Cruz, Wolf B Frommer","doi":"10.1094/MPMI-07-24-0075-FI","DOIUrl":"10.1094/MPMI-07-24-0075-FI","url":null,"abstract":"A path to sustainably reduce world hunger, food insecurity, and malnutrition is to close the crop yield gap and, particularly, lower losses due to pathogens. Breeding resistant crops is key to achieving this goal, which is an effort requiring collaboration among stakeholders, scientists, breeders, farmers, and policymakers. During a disease outbreak, epidemiologists survey the occurrence of a disease after which pathologists investigate mechanisms to stop an infection. Policymakers then implement strategies with farmers and breeders to overcome the outbreak. Information flow from the field to the lab and back to the field involves several processing hubs that require different information inputs. Failure to communicate the necessary information results in the transfer of meaningless data. Here, we discuss gaps in information acquisition and transfer between the field and laboratory. Using rice bacterial blight disease as an example, we discuss pathogen biology and disease resistance to point out the importance of reporting pathogen strains that caused an outbreak to optimize the deployment of resistant crop varieties. We examine differences between infection in the field and assays performed in the laboratory to draw awareness of possible misinformation concerning plant resistance or susceptibility. We discuss key data considered useful for reporting disease outbreaks, sampling bias, and suggestions for improving data quality. We also touch on the knowledge gap in the state-of-the-art literature regarding disease dispersal and transmission. We use a recent case study to exemplify the gaps mentioned. We conclude by highlighting potential actions that may contribute to food security and to closing the yield gap. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"134-141"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0