Molecular Plant-microbe Interactions最新文献_第3页

Unraveling the Fusarium oxysporum f. sp. fragariae-Strawberry Interaction: Evolution, Infection, and Genetic Resistance. 草莓尖孢镰刀菌互作：进化、感染和遗传抗性。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-08-07 DOI: 10.1094/MPMI-03-25-0028-IRW

Mishi V Vachev, Marta Bjornson, Dominique D A Pincot, Peter M Henry, Gitta L Coaker, Steven J Knapp, Mitchell J Feldmann

{"title":"Unraveling the Fusarium oxysporum f. sp. fragariae-Strawberry Interaction: Evolution, Infection, and Genetic Resistance.","authors":"Mishi V Vachev, Marta Bjornson, Dominique D A Pincot, Peter M Henry, Gitta L Coaker, Steven J Knapp, Mitchell J Feldmann","doi":"10.1094/MPMI-03-25-0028-IRW","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0028-IRW","url":null,"abstract":"Fusarium wilt of strawberry, caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. fragariae (Fof), is one of the greatest threats to cultivated strawberry. The pathogen penetrates strawberry plants through roots, severely affecting roots and crowns and resulting in rapid wilting and death. Research into the genetic basis of resistance to Fof has identified five loci, FW1 - FW5, that confer resistance to Fusarium wilt of strawberry and one Fof effector, SIX6. While it is hypothesized that FW1 recognizes the SIX6 effector, the underlying resistance gene is unknown. A new isolate of Fof that breaks FW1-mediated resistance recently emerged and poses a significant threat to the California strawberry industry, the source of 88-91% of the strawberries produced in the US. There are still significant gaps surrounding the molecular and physiological interaction between Fof and strawberry and the evolution of pathogenicity of Fof isolates unaffected by FW1. This review summarizes our current knowledge, identifies knowledge gaps, and provides a summary of genomic and molecular tools currently available to study the Fof-strawberry interaction.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenine Phosphoribosyltransferase Is a Universal Counter-Selectable Marker for DNA-Free Genome Editing in Oomycetes. 腺嘌呤磷酸核糖基转移酶是卵菌无dna基因组编辑的通用反选择标记。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-08-02 DOI: 10.1094/MPMI-05-25-0063-TA

Laurent Camborde, Chalisa Jaturapaktrarak, Jérôme Gouzy, Céline Lopez-Roques, Theerapong Krajaejun, Elodie Gaulin, Yacine Badis

{"title":"Adenine Phosphoribosyltransferase Is a Universal Counter-Selectable Marker for DNA-Free Genome Editing in Oomycetes.","authors":"Laurent Camborde, Chalisa Jaturapaktrarak, Jérôme Gouzy, Céline Lopez-Roques, Theerapong Krajaejun, Elodie Gaulin, Yacine Badis","doi":"10.1094/MPMI-05-25-0063-TA","DOIUrl":"https://doi.org/10.1094/MPMI-05-25-0063-TA","url":null,"abstract":"CRISPR-Cas genome editing is a powerful tool for understanding the pathogenicity of oomycetes, a group that includes several destructive plant parasites. While few Phytophthora species have benefited from plasmid-based transformation methods for gene overexpression and RNAi silencing, these techniques remain inefficient for other oomycete genera such as Pythium and Aphanomyces. Here, we explored the applicability of DNA-free endogenous counter-selection in filamentous oomycetes, using CRISPR-Cas9 ribonucleoproteins (RNPs). We used biolistics to deliver RNPs targeting the Adenine phosphoribosyltransferase (APT) gene, and generated selectable 2-fluoroadenine-resistant mutants in Aphanomyces, Pythium, and Phytophthora species. Targeted mutagenesis resulted in various deletions at the expected cut-sites, confirming efficient genome editing. Knockout mutants exhibited no alterations in growth or virulence, making APT a suitable selectable marker gene for oomycete research. Whole genome comparative analyses on CRISPR-edited mutants revealed no or very few additional mutations in A. euteiches and P. oligandrum, and substantial off-target effects in P. capsici. This one-step approach circumvents the need for protoplast generation and can be broadly applied to oomycetes producing zoospores or oospores.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Reference-Quality NLRome for the Hexaploid Sweetpotato and Diploid Wild Relatives. 六倍体甘薯和二倍体野生近缘NLRome的参考品质。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-12 DOI: 10.1094/MPMI-03-25-0034-R

C H Parada-Rojas, K L Childs, M Fernandez de Soto, A Salcedo, K Pecota, G C Yencho, C Almeyda, J P Hamilton, M Kitavi, C R Buell, G C Conant, D Baltzegar, L M Quesada-Ocampo

{"title":"A Reference-Quality NLRome for the Hexaploid Sweetpotato and Diploid Wild Relatives.","authors":"C H Parada-Rojas, K L Childs, M Fernandez de Soto, A Salcedo, K Pecota, G C Yencho, C Almeyda, J P Hamilton, M Kitavi, C R Buell, G C Conant, D Baltzegar, L M Quesada-Ocampo","doi":"10.1094/MPMI-03-25-0034-R","DOIUrl":"https://doi.org/10.1094/MPMI-03-25-0034-R","url":null,"abstract":"Breeding for sweetpotato (Ipomea batatas) resistance requires accelerating our understanding of genomic sources of resistance. Nucleotide-binding domain leucine-rich repeat receptors (NLRs) proteins represent a key component of the plant immune system that mediate plant immune responses. We cataloged the NLR diversity in 32 hexaploid sweetpotato genotypes and three diploid wild relatives using resistance gene enrichment sequencing (RenSeq) to capture and sequence full NLRs. A custom designed NLR bait-library enriched NLR genes with an average 97% target capture rate. We employed a curated database of cloned and functionally characterized NLRs to assign sequenced sweetpotato NLRs to canonical phylogenetic clades. We identified between 800 to 1,200 complete NLRs, highlighting the expanded diversity of coiled-coil NLRs (CNLs) across all genotypes. NLRs among sweetpotato genotypes exhibited large conservation across genotypes. Phylogenetic distance between 6X (hexaploid) and 2X (diploid) genotypes revealed that a small repertoire of I. batatas CNLs diverged from the sweetpotato wild relatives. Finally, we obtained chromosome coordinates in hexaploid (Beauregard) and diploid (Ipomoea trifida) genomes and recorded clustering of NLRs on chromosomes arms. Our study provides a catalog of NLR genes that can be used to accelerate breeding and increase our understanding of evolutionary dynamics of sweetpotato NLRs.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas9 Mutagenesis and Gene Overexpression to Enhance Resistance to Ascochyta medicaginicola in Medicago truncatula. CRISPR-Cas9诱变及基因过表达增强短叶紫花苜蓿对紫穗病的抗性

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-06 DOI: 10.1094/MPMI-05-25-0053-R

Jacob R Botkin, Shaun J Curtin

{"title":"CRISPR-Cas9 Mutagenesis and Gene Overexpression to Enhance Resistance to Ascochyta medicaginicola in Medicago truncatula.","authors":"Jacob R Botkin, Shaun J Curtin","doi":"10.1094/MPMI-05-25-0053-R","DOIUrl":"https://doi.org/10.1094/MPMI-05-25-0053-R","url":null,"abstract":"Alfalfa (Medicago sativa), the most widely cultivated forage legume globally is vulnerable to Ascochyta medicaginicola, the fungus causing spring black stem and leaf spot (SBS) disease which significantly reduces yield. SBS disease also affects Medicago truncatula, a diploid model legume with extensive genetic resources, including susceptible and resistant accessions. Using comparative genomics, four candidate genes for disease resistance were identified, MtTCAR1, MtPHO2A, MtCPR1-like, and MtPAM16. CRISPR/Cas9 mutagenesis was applied to generate independent mutant plants in the R108 accession and disease resistance was evaluated by a detached leaf qPCR-based pathogen assay. MtCPR1-like mutant plants exhibited a 34% reduction in pathogen biomass along with variable constitutive expression of pathogenesis-related genes. Additionally, a fifth candidate gene, MtKCS12, identified through transcriptomic analysis, was overexpressed in transformed plants resulting in 71.4-80.9% reduction in pathogen biomass compared to wild type segregants. This study validates gene-editing and transgenic approaches for improving SBS disease resistance in M. truncatula with future research focused on applying these strategies to enhance resistance in economically important alfalfa.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translocation of Effector Proteins into Plant Cells by the Flax Rust Pathogen Melampsora lini. 亚麻锈病病原菌黑腐菌效应蛋白在植物细胞中的易位。

IF 3.2 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-02 DOI: 10.1094/MPMI-12-24-0150-R

Xiaoxiao Zhang, Ann-Maree Catanzariti, Gregory J Lawrence, Pamela H P Gan, David A Jones, Peter N Dodds, John P Rathjen

{"title":"Translocation of Effector Proteins into Plant Cells by the Flax Rust Pathogen Melampsora lini.","authors":"Xiaoxiao Zhang, Ann-Maree Catanzariti, Gregory J Lawrence, Pamela H P Gan, David A Jones, Peter N Dodds, John P Rathjen","doi":"10.1094/MPMI-12-24-0150-R","DOIUrl":"https://doi.org/10.1094/MPMI-12-24-0150-R","url":null,"abstract":"During infection, rust fungi secrete effector proteins into host plant cells from haustoria to aid their colonisation. How rust effectors are secreted from the haustorium and delivered into the cytoplasm of host cells remains poorly understood. We used an Agrobacterium-mediated transformation procedure to generate stable transgenic flax rust strains expressing the effectors AvrM and AvrP123 fused to yellow fluorescent protein (YFP). We showed that both AvrM-YFP and AvrP123-YFP fusion proteins were secreted by the fungus into a narrow space surrounding the haustorium, likely the extrahaustorial matrix (EHMx); however only AvrM-YFP was delivered into host cells, triggering a typical resistance phenotype in plants carrying the corresponding resistance (R) gene M. The signal peptide of AvrM was sufficient to direct YFP secretion into the EHMx; however, delivery into the host cell required a larger 105 amino acid N-terminal fragment of AvrM. These results indicate that translocation of this protein into the host cell from the EHMx is a separate process from secretion into the EHMx and requires a signal present in AvrM between amino acids 34 and 105. This is in contrast to previous observations of AvrM localisation after transient expression in plants, highlighting the necessity for analysis in the natural infection system.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potato Virus Y Restricts Alternaria solani Growth During Co-Infection. 马铃薯Y病毒在共侵染过程中抑制茄交菌生长。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-01 Epub Date: 2025-07-31 DOI: 10.1094/MPMI-03-25-0026-R

Pablo A Gutierrez, Joshua Fuller, Sydney Stroschein, Austin VanDenTop, Dennis Halterman, Aurélie M Rakotondrafara

{"title":"Potato Virus Y Restricts Alternaria solani Growth During Co-Infection.","authors":"Pablo A Gutierrez, Joshua Fuller, Sydney Stroschein, Austin VanDenTop, Dennis Halterman, Aurélie M Rakotondrafara","doi":"10.1094/MPMI-03-25-0026-R","DOIUrl":"10.1094/MPMI-03-25-0026-R","url":null,"abstract":"In the environment, multiple microbes can interact with each other in the plant phyllosphere. These associations can shape the plant's development, stress responses, and disease susceptibility, but the molecular mechanisms that govern this process remain unexplained. Of interest are the multiple or successive infections that crop plants are exposed to within a growing season. One of the most common and economically important viruses of potato is potato virus Y (PVY, Potyviridae). We show that PVY infection of potato limited the expansion of foliar necrotic lesions caused by the early blight fungus Alternaria solani. The reduced growth phenotype persisted when the fungal mycelium was transferred to solid growth media. RNA-seq analysis of responses in potato and A. solani to the presence of PVY suggested two mechanisms that can explain this interaction. First, in A. solani exposed to PVY-positive leaves, we observed a downregulation of fungal pathogenicity genes. Second, we found that, in the absence of PVY, A. solani downregulates ethylene-responsive defense in potato, but this effect was eliminated when the host was infected with PVY. Our findings expand our understanding of how pathogen virulence can be affected by other pathogens competing for the same host resources. The observation that PVY can alter A. solani infection illustrates the ecological role of viruses as a potential contributor to the development of disease outbreaks. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"543-556"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

John Matthew McDowell: A Visionary Leader in Molecular Oomycete-Plant Interactions and a Wonderful Mentor and Friend to Many. 约翰·马修·麦克道尔：分子卵菌与植物相互作用的远见卓识领袖，许多人的良师益友。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-01 Epub Date: 2025-07-15 DOI: 10.1094/MPMI-06-25-0072-LE

Ryan G Anderson, Jeff H Chang, Jeffery L Dangl, Kasia Dinkeloo, Nicole Donofrio, Thomas Eulgem, Niklaus J Grünwald, John Herlihy, Terri Long, M Shahid Mukhtar, Guillaume Pilot, Sherif M Sherif, Unnati Sonawala, Mahmut Tör, Brenda Winkel, Boris A Vinatzer

引用次数: 0

SRF6 Is Necessary for the Perception of the Cell Wall Component TGA by Arabidopsis thaliana and Its Subsequent Immune Reaction. SRF6是拟南芥感知细胞壁成分TGA及其随后的免疫反应所必需的。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-01 Epub Date: 2025-08-12 DOI: 10.1094/MPMI-04-25-0036-R

Aparna Bhasin, Stephanie Chan, Ishal Dave, Leah Ritchie, G Adam Mott

{"title":"SRF6 Is Necessary for the Perception of the Cell Wall Component TGA by Arabidopsis thaliana and Its Subsequent Immune Reaction.","authors":"Aparna Bhasin, Stephanie Chan, Ishal Dave, Leah Ritchie, G Adam Mott","doi":"10.1094/MPMI-04-25-0036-R","DOIUrl":"10.1094/MPMI-04-25-0036-R","url":null,"abstract":"Plants are sessile organisms and must accurately respond to a variety of growth, developmental, and environmental signals throughout their life to maximize fitness. Plant cell surface receptor-like kinases are ideal for the perception of such signals and their transduction within the cell. The Strubbelig receptor family (SRF) is a group of leucine-rich repeat receptor-like kinases, several of which have unknown function. Here, we identify a role for SRF6 in the perception of cell wall damage and the activation of downstream immune responses. We show that SRF6 is necessary for proper immune responses following elicitation with a short-chain oligogalacturonic acid, including activation of defense genes and increased bacterial resistance. We also demonstrate that the srf6 mutants are more sensitive to isoxaben treatment, suggesting enhanced cell wall integrity maintenance responses. These findings are compatible with the hypothesis that cell wall integrity maintenance responses are elevated when pattern-triggered immunity is compromised. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"557-565"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Detour, Symbiotic Delay: Insights from Sinorhizobium meliloti Suppressor Mutants. 代谢迂回，共生延迟：来自中华根瘤菌meliloti抑制突变体的见解。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-01 DOI: 10.1094/MPMI-07-25-0078-CM

Jawahar Singh

引用次数: 0

Transcriptome and Cell Type Signature Analysis of Laser-Microdissected Syncytia Induced by the Cyst Nematode Heterodera schachtii in Arabidopsis Roots. 沙氏囊线虫诱导拟南芥根系激光显微解剖合胞体的转录组和细胞类型特征分析。

IF 3.4 3区生物学

Molecular Plant-microbe Interactions Pub Date : 2025-07-01 Epub Date: 2025-08-18 DOI: 10.1094/MPMI-03-25-0024-R

Xunliang Liu, Melissa G Mitchum

{"title":"Transcriptome and Cell Type Signature Analysis of Laser-Microdissected Syncytia Induced by the Cyst Nematode Heterodera schachtii in Arabidopsis Roots.","authors":"Xunliang Liu, Melissa G Mitchum","doi":"10.1094/MPMI-03-25-0024-R","DOIUrl":"10.1094/MPMI-03-25-0024-R","url":null,"abstract":"Cyst nematodes establish a highly specialized feeding structure called a syncytium in host roots by secreting effectors into a selected host cell that reprogram host development programs. The selected host cell undergoes distinct morphological, physiological, and gene expression changes, resulting in the fusion of hundreds of cells to create a novel cell type that does not normally exist in the host. Here, we profiled the transcriptome of the syncytium induced by the beet cyst nematode (BCN) Heterodera schachtii in Arabidopsis roots using laser capture microdissection and RNA sequencing. Aside from biological processes that are expected to be altered by nematode infection, we also found that genes annotated in nitrate and iron ion signaling and transport-related biological processes are significantly overrepresented in genes that are downregulated by BCN infection, suggesting that these ions may play important roles in BCN infection. Comparing the syncytium transcriptome with that of various root cell types showed that it was overrepresented by genes that are enriched in cells marked by ATHB15, a member of the HD-ZIP III transcription factor family that is highly expressed in the stem cell organizer of the root vasculature. These results suggest that the syncytium may partially adopt the molecular signature of a stem cell organizer, consistent with our previous hypothesis that BCN uses a stem cell organizer as an intermediate status for syncytium formation. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"529-542"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0